Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,116,511	T→G	I238L (ATT→CTT)	glpK ←	glycerol kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,116,511	0	T	G	100.0%	197.3 / NA	54	I238L (ATT→CTT)	glpK	glycerol kinase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (29/25);  total (29/25)

CGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTAC  >  NC_000913/4116367‑4116657                                                                                                                                                 |                                                                                                                                                   cGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAAGACGCGt                                                                                                                                              <  2:216795/151‑1 (MQ=255)      tCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAAGACGCGTgccgc                                                                                                                                         >  1:481784/1‑151 (MQ=255)           tttCACCGCTTTCTCGCCAGTGTTCATCAGCATAAAGCAGCCAGTTCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAAGACGCGTGCCGCCTTTg                                                                                                                                    >  2:106254/1‑151 (MQ=255)                 cGCTTTCTCGCCAGTGTTCATCAGCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAAGACGCGTGCCGCCTTTgccgcc                                                                                                                               >  2:834733/1‑150 (MQ=255)                 cGCTTTCTCGCCAGTGTTCATCAGCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAAGACGCGTGCCGCCTTTgccgcc                                                                                                                               >  1:222296/1‑150 (MQ=255)                    tttCTCGCCAGTGTTCATCAGCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAAGACGCGTGCCGCCTTTGCCGCCAATg                                                                                                                           >  2:601455/1‑151 (MQ=255)                      tctcGCCAGTGTTCATCAGCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAAGACGCGTGCCGCCTTTGCCGCCAATGtt                                                                                                                         >  2:246986/1‑151 (MQ=255)                         cGCCAGTGTTCATCAGCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAAGACGCGTGCCGCCTTTGCCGCCAATGTTAg                                                                                                                       <  2:525224/150‑1 (MQ=255)                           ccAGTGTTCATCAGCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAAGACGCGTGCCGCCTTTGCCGCCAATGTTAGTCt                                                                                                                    >  1:924717/1‑151 (MQ=255)                           ccAGTGTTCATCAGCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAAGACGCGTGCCGCCTTTGCCGCCAATGTTAGTCt                                                                                                                    >  2:138074/1‑151 (MQ=255)                                gtTCATCAGCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAAGACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCg                                                                                                               >  2:621795/1‑151 (MQ=255)                                    atcaGCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAAGACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGtat                                                                                                            >  2:158552/1‑150 (MQ=255)                                     tcaGCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAAGACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATAc                                                                                                          >  2:673522/1‑151 (MQ=255)                                      caGCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAAGACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACt                                                                                                         <  2:772289/151‑1 (MQ=255)                                         cATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAAGACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTc                                                                                                       >  2:408848/1‑150 (MQ=255)                                          aTAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAAGACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCgg                                                                                                     >  1:686205/1‑151 (MQ=255)                                          aTAAAGCAGCCAGTGCCATAGGCGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCTGCGATCCCGGAGATTGGAAGACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCgg                                                                                                     >  1:716504/1‑151 (MQ=255)                                            aaaGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAAGACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGgaa                                                                                                   <  1:411109/151‑1 (MQ=255)                                               gcagcCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAAGACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGgaagaa                                                                                                <  2:649273/151‑1 (MQ=255)                                               gcagcCAGTGCCATAGGTGTTCTTCGCCATCACTTCTTTCACGCACAAGTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGTGATTGGACGACGCGTGCCGCCTTTGCCACCAATGTTAGTCTGACCGTATACTTCGgaaga                                                                                                 >  1:848842/1‑150 (MQ=255)                                                cagcCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAAGACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGgaagaa                                                                                                <  1:4464/150‑1 (MQ=255)                                                cagcCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAAGACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAac                                                                                               >  2:553581/1‑151 (MQ=255)                                                cagcCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAAGACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAac                                                                                               >  1:401469/1‑151 (MQ=255)                                                  gcCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAAGACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAacga                                                                                             <  2:329721/151‑1 (MQ=255)                                                  gcCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAAGACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAacga                                                                                             <  1:418862/151‑1 (MQ=255)                                                   ccAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAAGACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAacgac                                                                                            <  2:829396/151‑1 (MQ=255)                                                     aGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAAGACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGc                                                                                          >  1:559484/1‑151 (MQ=255)                                                        gCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAAGACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACt                                                                                       <  2:14338/151‑1 (MQ=255)                                                            tAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAAGACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTg                                                                                   >  2:499161/1‑151 (MQ=255)                                                                  ttcttcGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAAGACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGgca                                                                                >  2:629847/1‑148 (MQ=255)                                                                    cttcGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAAGACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCAtc                                                                           >  1:922316/1‑151 (MQ=255)                                                                    cttcGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAAGACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCAtc                                                                           >  2:676168/1‑151 (MQ=255)                                                                      tcGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAAGACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCAt                                                                            <  2:794882/148‑1 (MQ=255)                                                                       cGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGTGATTGGAAGACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCTtctc                                                                         <  1:345496/150‑1 (MQ=255)                                                                          cATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAAGACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTcgcgc                                                                     >  1:222095/1‑151 (MQ=255)                                                                             cccTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAAGACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCgg                                                                   <  2:368234/150‑1 (MQ=255)                                                                                      cACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAAGACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAg                                                          <  1:917418/150‑1 (MQ=255)                                                                                      cACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAAGACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAg                                                          <  1:540769/150‑1 (MQ=255)                                                                                        cGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAAGACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCa                                                        >  2:703323/1‑150 (MQ=255)                                                                                        cGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAAGACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCAc                                                       <  1:62045/151‑1 (MQ=255)                                                                                           acaACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAAGACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTc                                                    >  2:114069/1‑151 (MQ=255)                                                                                           acaACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAAGACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTc                                                    >  1:227376/1‑151 (MQ=255)                                                                                                  aCCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAAGACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCAtt                                             <  1:118241/151‑1 (MQ=255)                                                                                                   ccAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAAGACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGc                                                <  2:119399/147‑1 (MQ=255)                                                                                                   ccAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAAGACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCAttt                                            <  1:113011/151‑1 (MQ=255)                                                                                                    cAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAAGACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCAtttt                                           >  2:112613/1‑151 (MQ=255)                                                                                                    cAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAAGACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCAtttt                                           <  1:128788/151‑1 (MQ=255)                                                                                                        cAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAAGACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTgtc                                        <  1:903061/150‑1 (MQ=255)                                                                                                          gcgcGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAAGACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTgtcgtc                                     <  2:518358/151‑1 (MQ=255)                                                                                                                           ccGGCGATCCCGGAGATTGGAAGACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGa                    <  2:285691/151‑1 (MQ=255)                                                                                                                              gCGATCCCGGAGATTGGAAGACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATg                  >  1:587459/1‑150 (MQ=255)                                                                                                                                   cccGGAGATTGGAAGACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGaa             <  1:621775/150‑1 (MQ=255)                                                                                                                                    ccGGAGATTGGAAGACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGaac            >  2:731526/1‑150 (MQ=255)                                                                                                                                             ggAAGACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTAc  <  1:511248/151‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                                   CGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTAC  >  NC_000913/4116367‑4116657

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑