Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,146,701	A→C	S148S (TCA→TCC)	pflC →	putative [formate‑C‑acetyltransferase 2]‑activating enzyme; pyruvate formate‑lyase 1‑activating enzyme

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,146,701	0	A	C	100.0%	229.5 / NA	63	S148S (TCA→TCC)	pflC	putative [formate‑C‑acetyltransferase 2]‑activating enzyme; pyruvate formate‑lyase 1‑activating enzyme
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (35/28);  total (35/28)

GCCTTGACGCTCTGGAACGGGAAGTGATGAAAGATGACATTTTCTTTCGCACGTCCGGCGGCGGCGTCACGCTTTCTGGCGGCGAAGTGTTAATGCAGGCGGAGTTTGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGTCATGCGCCATTGAAACTGCCGGAGACGCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGCGGGATGTGGTGAAGATGAACCTGCCACGCGT  >  NC_000913/4146553‑4146841                                                                                                                                                     |                                                                                                                                             gCCTTGACGCTCTGGAACGGGAAGTGATGAAAGATGACATTTTCTTTCGCACGTCCGGCGGCGGCGTCACGCTTTCTGGCGGCGAAGTGTTAATGCAGGCGGAGTTTGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGTCCTg                                                                                                                                            <  1:163473/151‑1 (MQ=255)  ccTTGACGCTCTGGAACGGGAAGTGATGAAAGATGACATTTTCTTTCGCACGTCCGGCGGCGGCGTCACGCTTTCTGGCGGCGAAGTGTTAATGCAGGCGGAGTTTGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGTCCTgc                                                                                                                                           <  2:64759/151‑1 (MQ=255)          ctctGGAACGGGAAGTGATGAAAGATGACATTTTCTTTCGCACGTCCGGCGGCGGCGTCACGCTTTCTGGCGGCGAAGTGTTAATGCAGGCGGAGTTTGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGTCCTGCGCCATTg                                                                                                                                    >  1:499051/1‑150 (MQ=255)            ctGGAACGGGAAGTGATGAAAGATGACATTTTCTTTCGCACGTCCGGCGGCGGCGTCACGCTTTCTGGCGGCGAAGTGTTAATGCAGGCGGAGTTTGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGTCCTGCGCCATTGaa                                                                                                                                  >  1:886188/1‑150 (MQ=255)              ggAACGGGAAGTGATGAAAGATGACATTTTCTTTCGCACGTCCGGCGGCGGCGTCACGCTTTCTGGCGGCGAAGTGTTAATGCAGGCGGAGTTTGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGTCCTGCGCCATTGAAACt                                                                                                                               <  2:372845/151‑1 (MQ=255)              ggAACGGGAAGTGATGAAAGATGACATTTTCTTTCGCACGTCCGGCGGCGGCGTCACGCTTTCTGGCGGCGAAGTGTTAATGCAGGCGGAGTTTGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGTCCTGCGCCATTGAAACt                                                                                                                               >  1:469113/1‑151 (MQ=255)              ggAACGGGAAGTGATGAAAGATGACATTTTCTTTCGCACGTCCGGCGGCGGCGTCACGCTTTCTGGCGGCGAAGTGTTAATGCAGGCGGAGTTTGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGTCCTGCGCCATTGAAACt                                                                                                                               <  1:49496/151‑1 (MQ=255)                aaCGGGAAGTGATGAAAGATGACATTTTCTTTCGCACGTCCGGCGGCGGCGTCACGCTTTCTGGCGGCGAAGTGTTAATGCAGGCGGAGTTTGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGTCCTGCGCCATTGAAACTGc                                                                                                                             >  2:895168/1‑151 (MQ=255)                   gggAAGTGATGAAAGATGACATTTTCTTTCGCACGTCCGGCGGCGGCGTCACGCTTTCTGGCGGCGAAGTGTTAATGCAGGCGGAGTTTGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGTCCTGCGCCATTGAAACTGCCgg                                                                                                                          >  1:160351/1‑151 (MQ=255)                        gTGATGAAAGATGGCATTTTCTTTCGCACGTCCGGCGGCGGCGTCACGCTTTCTGGCGGCGAAGTGTTAATGCAGGCGGAGTTTGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGTCCTGCGCCATTGAAACTGCCGGAGAc                                                                                                                      <  1:746046/150‑1 (MQ=255)                              aaaGATGACATTTTCTTTCGCACGTCCGGCGGCGGCGTCAGGCTTTCTGGCGGCGAAGTGTTAATCCAGGCGGATTTTGCTACCCGGTTTTTACAGCGACTGCGGCTGGGGGGGGTGGGCGGCGCCATTGAAACTGCCGAAGACGc                                                                                                                    >  1:838122/1‑146 (MQ=255)                              aaaGATGACATTTTCTTTCGCACGTCCGGCGGCGGCGTCACGCTTTCTGGCGGCGAAGTGTTAATGCAGGCGGAGTTTGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGGGTGTCCTGCGCCATTGAAACTGCCGGAGACGCAc                                                                                                                  >  1:904747/1‑148 (MQ=255)                                  aTGACATTTTCTTTCGCACGTCCGGCGGCGGCGTCACGCTTTCTGGCGGCGAAGTGTTAATGCAGGCGGAGTTTGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGTCCTGCGCCATTGAAACTGCCGGAGACGCAccagccag                                                                                                           <  2:76398/151‑1 (MQ=255)                                     aCATTTTCTTTCGCACGTCCGGCGGCGGCGTCACGCTTTCTGGCGGCGAAGTGTTAATGCAGGCGGAGTTTGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGTCCTGCGCCATTGAAACTGCCGGAGACGCACCAGCCAGCa                                                                                                         >  2:304519/1‑150 (MQ=255)                                           tctttcGCACGTCCGGCGGCGGCGTCACGCTTTCTGGCGGCGAAGTGTTAATGCAGGCGGAGTTTGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGTCCTGCGCCATTGAAACTGCCGGAGACGCACCAGCCAGCAAGCTAtt                                                                                                  >  2:811064/1‑151 (MQ=255)                                           tctttcGCACGTCCGGCGGCGGCGTCACGCTTTCTGGCGGCGAAGTGTTAATGCAGGCGGAGTTTGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGTCCTGCGCCATTGAAACTGCCGGAGACGCACCAGCCAGCAAGCTAt                                                                                                   <  1:350738/150‑1 (MQ=255)                                            ctttcGCACGTCCGGCGGCGGCGTCACGCTTTCTGGCGGCGAAGTGTTAATGCAGGCGGAGTTTGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGTCCTGCGCCATTGAAACTGCCGGAGACGCACCAGCCAGCAAGCTAtt                                                                                                  >  1:258129/1‑150 (MQ=255)                                                 gCACGTCCGGCGGCGGCGTCACGCTTTCTGGCGGCGAAGTGTTAATGCAGGCGGAGTTTGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGTCCTGCGCCATTGAAACTGCCGGAGACGCACCAGCCAGCAAGCTATTACCGCt                                                                                            <  1:628438/151‑1 (MQ=255)                                                  cACGTCCGGCGGCGGCGTCACGCTTTCTGGCGGCGAAGTGTTAATGCAGGCGGAGTTTGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGTCCTGCGCCATTGAAACTGCCGGAGACGCACCAGCCAGCAAGCTATTACCGCTg                                                                                           <  2:902893/151‑1 (MQ=255)                                                   aCGTCCGGCGGCGGCGTCACGCTTTCTGGCGGCGAAGTGTTAATGCAGGCGGAGTTTGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGTCCTGCGCCATTGAAACTGCCGGAGACGCACCAGCCAGCAAGCTATTACCGCTgg                                                                                          <  2:831361/151‑1 (MQ=255)                                                     gTCCGGCGGCGGCGTCACGCTTTCTGGCGGCGAAGTGTTAATGCAGGCGGAGTTTGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGTCCTGCGCCATTGAAACTGCCGGAGACGCACCAGCCAGCAAGCTATTACCGCTGGCg                                                                                        <  1:33084/151‑1 (MQ=255)                                                     gTCCGGCGGCGGCGTCACGCTTTCTGGCGGCGAAGTGTTAATGCAGGCGGAGTTTGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGTCCTGCGCCATTGAAACTGCCGGAGACGCACCAGCCAGCAAGCTATTACCGCTGGCg                                                                                        >  2:922615/1‑151 (MQ=255)                                                       ccGGCGGCGGCGTCACGCTTTCTGGCGGCGAAGTGTTAATGCAGGCGGAGTTTGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGTCCTGCGCCATTGAAACTGCCGGAGACGCACCAGCCAGCAAGCTATTACCGCTGGCGa                                                                                       >  2:731521/1‑150 (MQ=255)                                                       ccGGCGGCGGCGTCACGCTTTCTGGCGGCGAAGTGTTAATGCAGGCGGAGTTTGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGTCCTGCGCCATTGAAACTGCCGGAGACGCACCAGCCAGCAAGCTATTACCGCTGGCGa                                                                                       >  2:172371/1‑150 (MQ=255)                                                        cggcggcggCGTCACGCTTTCTGGCGGCGAAGTGTTAATGCAGGCGGAGTTTGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGTCCTGCGCCATTGAAACTGCCGGAGACGCACCAGCCAGCAAGCTATTACCGCTGGCGaa                                                                                      >  2:657991/1‑150 (MQ=255)                                                            ggcggcgTCACGCTTTCTGGCGGCGAAGTGTTAATGCAGGCGGAGTTTGCTCCCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGTCCTGCGCCATTGAAACTGCCGGAGACGCACCAGCCAGCAAGCTATTACCGCTGGCGAAATtg                                                                                  <  1:576180/150‑1 (MQ=255)                                                            ggcggcgTCACGCTTTCTGGCGGCGAAGTGTTAATGCAGGCGGAGTTTGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGTCCTGCGCCATTGAAACTGCCGGAGACGCACCAGCCAGCAAGCTATTACCGCTGGCGAAATtg                                                                                  >  1:610702/1‑150 (MQ=255)                                                             gcggcgTCACGCTTTCTGGCGGCGAAGTGTTAATGCAGGCGGAGTTTGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGTCCTGCGCCATTGAAACTGCCGGAGACGCACCAGCCAGCAAGCTATTACCGCTGGCGAAATtgtg                                                                                >  1:551410/1‑151 (MQ=255)                                                             gcggcgTCACGCTTTCTGGCGGCGAAGTGTTAATGCAGGCGGAGTTTGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGTCCTGCGCCATTGAAACTGCCGGAGACGCACCAGCCAGCAAGCTATTACCGCTGGCGAAATtgtg                                                                                >  2:800898/1‑151 (MQ=255)                                                             gcggcgTCACGCTTTCTGGCGGCGAAGTGTTAATGCAGGCGGAGTTTGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGTCCTGCGCCATTGAAACTGCCGGAGACGCACCAGCCAGCAAGCTATTACCGCTGGCGAAATtgtg                                                                                <  1:373722/151‑1 (MQ=255)                                                                gcgTCACGCTTTCTGGCGGCGAAGTGTTAATGCAGGCGGAGTTTGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGTCCTGCGCCATTGAAACTGCCGGAGACGCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGa                                                                             <  2:384242/151‑1 (MQ=255)                                                                   tCACGCTTTCTGGCGGCGAAGTGTTAATGCAGGCGGAGTTTGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGTCCTGCGCCATTGAAACTGCCGGAGACGCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGATg                                                                           >  2:498853/1‑150 (MQ=255)                                                                    cACGCTTTCTGGCGGCGAAGTGTTAATGCAGGCGGAGTTTGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGTCCTGCGCCATTGAAACTGCCGGAGACGCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGATGa                                                                          >  2:290096/1‑150 (MQ=255)                                                                    cACGCTTTCTGGCGGCGAAGTGTTAATGCAGGCGGAGTTTGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGTCCTGCGCCATTGAAACTGCCGGAGACGCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGATGa                                                                          <  2:926143/150‑1 (MQ=255)                                                                      cGCTTTCTGGCGGCGAAGTGTTAATGCAGGCGGAGTTTGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGTCCTGCGCCATTGAAACTGCCGGAGACGCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGATGAAg                                                                        >  2:640555/1‑150 (MQ=255)                                                                           tCTGGCGGCGAAGTGTTAATGCAGGCGGAGTTTGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGTCCTGCGCCATTGAAACTGCCGGAGACGCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGATGAAGtgttgt                                                                  >  2:650123/1‑151 (MQ=255)                                                                              ggcggcGAAGTGTTAATGCAGGCGGAGTTTGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGTCCTGCGCCATTGAAACTGCCGGAGACGCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGATGAAGTgttgtt                                                                 <  1:460076/149‑1 (MQ=255)                                                                                  gcgAAGTGTTAATGCAGGCGGAGTTTGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGTCCTGCGCCATTGAAACTGCCGGAGACGCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGAttt                                                           <  1:324861/151‑1 (MQ=255)                                                                                    gAAGTGTTAATGCAGGCGGAGTTTGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGTCCTGCGCCATTGAAACTGCCGGAGACGCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTaa                                                         <  1:523979/151‑1 (MQ=255)                                                                                    gAAGTGTTAATGCAGGCGGAGTTTGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGTCCTGCGCCATTGAAACTGCCGGAGACGCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTa                                                          >  2:756523/1‑150 (MQ=255)                                                                                    gAAGTGTTAATGCAGGCGGAGTTTGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGTCCTGCGCCATTGAAACTGCCGGAGACGCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTa                                                          >  2:482086/1‑150 (MQ=255)                                                                                         gtTAATGCAGGCGGAGTTTGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGTCCTGCGCCATTGAAACTGCCGGAGACGCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAAtt                                                    <  2:467868/151‑1 (MQ=255)                                                                                             aTGCAGGCGGAGTTTGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGTCCTGCGCCATTGAAACTGCCGGAGACGCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAATTAt                                                  >  2:542487/1‑149 (MQ=255)                                                                                               gCAGGCGGAGTTTGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGTCCTGCGCCATTGAAACTGCCGGAGACGCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAATTATGGa                                               <  2:731806/150‑1 (MQ=255)                                                                                               gCAGGCGGAGTTTGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGTCCTGCGCCATTGAAACTGCCGGAGACGCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAATTATGGa                                               <  1:71189/150‑1 (MQ=255)                                                                                               gCAGGCGGAGTTTGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGTCCTGCGCCATTGAAACTGCCGGAGACGCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAATTATGGAc                                              <  1:725777/151‑1 (MQ=255)                                                                                               gCAGGCGGAGTTTGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGTCCTGCGCCATTGAAACTGCCGGAGACGCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAATTATGGAc                                              >  2:234161/1‑151 (MQ=255)                                                                                                 aGGCGGAGTTTGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGTCCTGCGCCATTGAAACTGCCGGAGACGCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAATTATGGAcgc                                            <  1:792850/151‑1 (MQ=255)                                                                                                     ggAGTTTGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGTCCTGCGCCATTGAAACTGCCGGAGACGCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAATTATGGACGCGACt                                        >  1:304722/1‑151 (MQ=255)                                                                                                          ttGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGTCCTGCGCCATTGAAACTGCCGGAGACGCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAATTATGGACGCGACTCAgg                                    <  1:259145/150‑1 (MQ=255)                                                                                                           tGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGTCCTGCGCCATTGAAACTGCCGGAGACGCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAATTATGGACGCGACTCAGgcg                                  >  2:774871/1‑151 (MQ=255)                                                                                                               aCCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGTCCTGCGCCATTGAAACTGCCGGAGACGCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGCggg                              >  1:507924/1‑151 (MQ=255)                                                                                                                 ccGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGTCCTGCGCCATTGAAACTGCCGGAGACGCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGCGGGa                             >  2:307348/1‑150 (MQ=255)                                                                                                                       tttACAGCGACTGCGGCTGTGGGGTGTGTCCTGCGCCATTGAAACTGCCGGAGACGCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGCGGGATgtggtg                      >  1:63334/1‑151 (MQ=255)                                                                                                                         tACAGCGACTGCGGCTGTGGGGTGTGTCCTGCGCCATTGAAACTGCCGGAGACGCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGCGGGATGTGGTGa                     >  1:506996/1‑150 (MQ=255)                                                                                                                          aCAGCGACTGCGGCTGTGGGGTGTGTCCTGCGCCATTGAAACTGCCGGAGACGCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAGTTATGGACGCGACTCAGGCGCGGGATGGGGTGa                     >  1:170653/1‑149 (MQ=255)                                                                                                                           cAGCGACTGCGGCTGTGGGGTGTGTCCTGCGCCATTGAAACTGCCGGAGACGCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGCGGGATgtggtg                      >  1:419213/1‑147 (MQ=255)                                                                                                                             gCGACTGCGGCTGTGGGGTGTGTCCTGCGCCATTGAAACTGCCGGAGACGCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGCGGGATGTGGTGAAGATg                <  1:469658/151‑1 (MQ=255)                                                                                                                               gACTGCGGCTGTGGGGTGTGTCCTGCGCCATTGAAACTGCCGGAGACGCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGCGGGATGTGGTGAAGATGaa              <  1:895147/151‑1 (MQ=255)                                                                                                                               gACTGCGGCTGTGGGGTGTGTCCTGCGCCATTGAAACTGCCGGAGACGCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGCGGGATGTGGTGAAGATGa               >  1:704439/1‑150 (MQ=255)                                                                                                                                        tgtgGGGTGTGTCCTGCGCCATTGAAACTGCCGGAGACGCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGCGGGATGTGGTGAAGATGAACCTGCCAcg     >  1:863411/1‑151 (MQ=255)                                                                                                                                           ggggTGTGTCCTGCGCCATTGAAACTGCCGGAGACGCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGCGGGATGTGGTGAAGATGAACCTGCCACGCGt  <  1:432357/151‑1 (MQ=255)                                                                                                                                           ggggTGTGTCCTGCGCCATTGAAACTGCCGGAGACGCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGCGGGATGTGGTGAAGATGAACCTGCCACGCGt  <  1:843344/151‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                             GCCTTGACGCTCTGGAACGGGAAGTGATGAAAGATGACATTTTCTTTCGCACGTCCGGCGGCGGCGTCACGCTTTCTGGCGGCGAAGTGTTAATGCAGGCGGAGTTTGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGTCATGCGCCATTGAAACTGCCGGAGACGCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGCGGGATGTGGTGAAGATGAACCTGCCACGCGT  >  NC_000913/4146553‑4146841

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑