Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	235,475	A→C	K220N (AAA→AAC)	yafS →	putative S‑adenosyl‑L‑methionine‑dependent methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	235,475	0	A	C	100.0%	190.0 / NA	51	K220N (AAA→AAC)	yafS	putative S‑adenosyl‑L‑methionine‑dependent methyltransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (29/22);  total (29/22)

ACGCCAGCCGTTTCCACGTTCTCCCGTGGAACAAACACGGAGGAAAACTATTGAATGCGCATATTCCTGCGCTTGGTTGCTTACAACTTATTGTTGCCCGGAAACGGACTATTCCTTTAACGCTAAATCCGATGAAACAGAGTAAAAACAAGCCACGAATTCGCCAGGCGGTTGGAGCCACCCGGCAATGTCGTAAACCACAGGCTTAAACTTCAACTTGGTAGCCTGTATCTTCCAGTGTGGGATTCATCGCCGCGGCACGAGCCAGTTCATCACAGCGTTCGTTTTCCGGG  >  NC_000913/235330‑235622                                                                                                                                                  |                                                                                                                                                    acGCCAGCCGTTTCCACGTTCTCCCGTGGAACAAACACGGAGGAAAACTATTGAATGCGCATATTCCTGCGCTTGGTTGCTTACAACTTATTGTTGCCCGGAAACGGACTATTCCTTTAACGCTAAATCCGATGAAACAGAGTAAcaacaa                                                                                                                                                <  1:589195/151‑1 (MQ=255)  cGCCAGCCGTTTCCACGTTCTCCCGTGGAACAAACACGGAGGAAAACTATTGAATGCGCATATTCCTGCGCTTGGTTGCTTACAACTTATTGTTGCCCGGAAACGGACTATTCCTTTAACGCTAAATCCGATGAAACAGAGTAAcaacaa                                                                                                                                                <  1:151036/150‑1 (MQ=255)   gCCAGCCGTTTCCACGTTCTCCCGTGGAACAAACACGGAGGAAAACTATTGAATGCGCATATTCCTGCGCTTGGTTGCTTACAACTTATTGTTGCCCGGAAACGGACTATTCCTTTAACGCTAAATCCGATGAAACAGAGTAACAACAAGc                                                                                                                                              >  1:327022/1‑151 (MQ=255)    ccAGCCGTTTCCACGTTCTCCCGTGGAACAAACACGGAGGAAAACTATTGAATGCGCATATTCCTGCGCTTGGTTGCTTACAACTTATTGTTGCCCGGAAACGGACTATTCCTTTAACGCTAAATCCGATGAAACAGAGTAACAACAAGcc                                                                                                                                             <  1:636544/151‑1 (MQ=255)                          gTGGAACAAACACGGAGGAAAACTATTGAATGCGCATATTCCTGCGCTTGGTTGCTTACAACTTATTGTTGCCCGGAAACGGACTATTCCTTTAACGCTAAATCCGATGAAACAGAGTAACAACAAGCCACGAATTCGCCAGGCGGTTgg                                                                                                                        >  1:354328/1‑150 (MQ=255)                              aacaaacaCGGAGGAAAACTATTGAATGCGCATATTCCTGCGCTTGGTTGCTTACAACTTATTGTTGCCCGGAAACGGACTATTCCTTTAACGCTAAATCCGATGAAACAGAGTAACAACAAGCCACGAATTCGCCAGGCGGTTGGAGCCa                                                                                                                   <  2:527400/151‑1 (MQ=255)                                  aacaCGGAGGAAAGCTATTGAATGCGCATATTCCTGCGCTTGGTTGCTTACAACTTATTGTTGCCCGGAAACGGACTATTCCTTTAACGCTAAATCCGATGAAACAGAGTAACAACAAGCCACGAATTCGCCAGGCGGTTGGAGCCTccc                                                                                                                <  1:5341/150‑1 (MQ=255)                                  aacaCGGAGGAAAACTATTGAATGCGCATATTCCTGCGCTTGGTTGCTTACAACTTATTGTTGCCCGGAAACGGACTATTCCTTTAACGCTAAATCCGATGAAACAGAGTAACAACAAGCCACGAATTCGCCAGGCGGTTGGAGCCACCCg                                                                                                               >  1:790193/1‑151 (MQ=255)                                   acacGGAGGAAAACTATTGAATGCGCATATTCCTGCGCTTGGTTGCTTACAACTTATTGTTGCCCGGAAACGGACTATTCCTTTAACGCTAAATCCGATGAAACAGAGTAACAACAAGCCACGAATTCGCCAGGCGGTTGGAGCCACCCgg                                                                                                              <  1:124649/151‑1 (MQ=255)                                      cGGAGGAAAACTATTGAATGCGCATATTCCTGCGCTTGGTTGCTTACAACTTATTGTTGCCCGGAAACGGACTATTCCTTTAACGCTAAATCCGATGAAACAGAGTAACAACAAGCCACGAATTCGCCAGGCGGTTGGAGCCACCCGGCaa                                                                                                           <  1:829882/151‑1 (MQ=255)                                          ggaAAACTATTGAATGCGCATATTCCTGCGCTTGGTTGCTTACAACTTATTGTTGCCCGGAAACGGACTATTCCTTTAACGCTAAATCCGATGAAACAGAGTAACAACAAGCCACGAATTCGCCAGGCGGTTGGAGCCACCCGGCAATGTc                                                                                                       <  1:450670/151‑1 (MQ=255)                                          ggaAAACTATTGAATGCGCATATTCCTGCGCTTGGTTGCTTACAACTTATTGTTGCCCGGAAACGGACTATTCCTTTAACGCTAAATCCGATGAAACAGAGTAACAACAAGCCACGAATTCGCCAGGCGGTTGGAGCCACCCGGCAATGTc                                                                                                       <  1:795924/151‑1 (MQ=255)                                            aaaaCTATTGAATGCGCATATTCCTGCGCTTGGTTGGTTACAACTTATTGTTGCCCGGAAACGGACTATTCCTTTAACGCTAAATCCGATGAAACAGAGTAACAACAAGCCACGAATTCGCCAGGCGGCTGGAGCCACCCGGCAATGTCGt                                                                                                     >  1:389655/1‑151 (MQ=255)                                                 tATTGAATGCGCATATTCCTGCGCTTGGTTGCTTACAACTTATTGTTGCCCGGAAACGGACTATTCCTTTAACGCTAAATCCGATGAAACAGAGTAACAACAAGCCACGAATTCGCCAGGCGGTTGGAGCCACCCGGCAATGTCGTAAAcc                                                                                                >  2:537773/1‑151 (MQ=255)                                                  aTTGAATGCGCATATTCCTGCGCTTGGTTGCTTACAACTTATTGTTGCCCGGAAACGGACTATTCCTTTAACGCTAAATCCGATGAAACAGAGTAACAACAAGCCACGAATTCGCCAGGCGGTTGGAGCCACCCGGCAATGTCGTAAAc                                                                                                 <  2:63870/149‑1 (MQ=255)                                                  aTTGAATGCGCATATTCCTGCGCTTGGTTGCTTACAACTTATTGTTGCCCGGAAACGGACTATTCCTTTAACGCTAAATCCGATGAAACAGAGTAACAACAAGCCACGAATTCGCCAGGCGGTTGGAGCCACCCGGCAATGTCGTAAAc                                                                                                 <  2:379581/149‑1 (MQ=255)                                                       attcgcATATTCCTGCGCTTGGTTGCTTACAACTTATTGTTGCCCGGAAACGGACTATTCCTTTAACGCTAAATCCGATGAAACAGAGTAACAACAAGCCACGAATTCGCCAGGCGGTTGGAGCCACCCGGCAATGTCGTAAACCACAg                                                                                            <  2:578873/146‑1 (MQ=255)                                                           gcATATTCCTGCGCTTGGTTGCTTACAACTTATTGTTGCCCGGAAACGGACTATTCCTTTAACGCTAAATCCGATGAAACAGAGTAACAACAAGCCACGAATTCGCCAGGCGGTTGGAGCCACCCGGCAATGTCGTAAACCACAGGCTTa                                                                                       >  2:870007/1‑150 (MQ=255)                                                            cATATTCCTGCGCTTGGTTGCTTACAACTTATTGTTGCCCGGAAACGGACTATTCCTTTAACGCTAAATCCGATGAAACAGAGTAACAACAAGCCACGAATTCGCCAGGCGGTTGGAGCCACCCGGCAATGTCGTAAACCACAGGCTTaa                                                                                      >  1:446734/1‑150 (MQ=255)                                                              tatTCCTGCGCTTGGTTGCTTACAACTTATTGTTGCCCGGAAACGGACTATTCCTTTAACGCTAAATCCGATGAAACAGAGTAACAACAAGCCACGAATTCGCCAGGCGGTTGGAGCCACCCGGCAATGTCGTAAACCACAGGCTTAAACt                                                                                   >  2:213104/1‑151 (MQ=255)                                                              tatTCCTGCGCTTGGTTGCTTACAACTTATTGTTGCCCGGAAACGGACTATTCCTTTAACGCTAAATCCGATGAAACAGAGTAACAACAAGCCACGAATTCGCCAGGCGGTTGGAGCCACCCGGCAATGTCGTAAACCACAGGCTTAAACt                                                                                   >  1:196327/1‑151 (MQ=255)                                                               atTCCTGCGCTTGGTTGCTTACAACTTATTGTTGCCCGGAAACGGACTATTCCTTTAACGCTAAATCCGATGAAACAGAGTAACAACAAGCCACGAATTCGCCAGGCGGTTGGAGCCACCCGGCAATGTCGTAAACCACAGGCTTAAACtt                                                                                  >  1:289084/1‑151 (MQ=255)                                                                 tCCTGCGCTTGGTTGCTTACAACTTATTGTTGCCCGGAAACGGACTATTCCTTTAACGCTAAATCCGATGAAACAGAGTAACAACAAGCCACGAATTCGCCAGGCGGTTGGAGCCACCCGGCAATGTCGTAAACCACAGGCTTAAACTTc                                                                                 >  2:296526/1‑150 (MQ=255)                                                                   cTGCGCTTGGTTGCTTACAACTTATTGTTGCCCGGAAACGGACTATTCCTTTAACGCTAAATCCGATGAAACAGAGTAACAACAAGCCACGAATTCGCCAGGCGGTTGGAGCCACCCGGCAATGTCGTAAACCACAGGCTTAAACTTCAAc                                                                              >  2:567030/1‑151 (MQ=255)                                                                     gcgcTTGGTTGCTTACAACTTATTGTTGCCCGGAAACGGACTATTCCTTTAACGCTAAATCCGATGAAACAGAGTAACAACAAGCCACGAATTCGCCAGGCGGTTGGAGCCACCCGGCAATGTCGTAAACCACAGGCTTAAACTTCAAc                                                                              <  2:658271/149‑1 (MQ=255)                                                                     gcgcTTGGTTGCTTACAACTTATTGTTGCCCGGAAACGGACTATTCCTTTAACGCTAAATCCGATGAAACAGAGTAACAACAAGCCACGAATTCGCCAGGCGGTTGGAGCCACCCGGCAATGTCGTAAACCACAGGCTTAAACTTCAACtt                                                                            >  2:278149/1‑151 (MQ=255)                                                                           ggTTGCTTACAACTTATTGTTGCCCGGAAACGGACTATTCCTTTAACGCTAAATCCGATGAAACAGAGTAACAACAAGCCACGAATTCGCCAGGCGGTTGGAGCCACCCGGCAATGTCGTAAACCACAGGCTTAAACTTCAACTTGGTAGc                                                                      >  1:561234/1‑151 (MQ=255)                                                                              tGCTTACAACTTATTGTTGCCCGGAAACGGACTATTCCTTTAACGCTAAATCCGATGAAACAGAGTAACAACAAGCCACGAATTCGCCAGGCGGTTGGAGCCACCCGGCAATGTCGTAAACCACAGGCTTAAACTTCAACTTGGTAGCCt                                                                    <  1:427329/150‑1 (MQ=255)                                                                              tGCTTACAACTTATTGTTGCCCGGAAACGGACTATTCCTTTAACGCTAAATCCGATGAAACAGAGTAACAACAAGCCACGAATTCGCCAGGCGGTTGGAGCCACCCGGCAATGTCGTAAACCACAGGCTTAAACTTCAACTTGGTAGCCTg                                                                   >  2:523323/1‑151 (MQ=255)                                                                               gCTTACAACTTATTGTTGCCCGGAAACGGACTATTCCTTTAACGCTAAATCCGATGAAACAGAGTAACAACAAGCCACGAATTCGCCAGGCGGTTGGAGCCACCCGGCAATGTCGTAAACCACAGGCTTAAACTTCAACTTGGTAGCCTGt                                                                  >  1:659712/1‑151 (MQ=255)                                                                                     aaCTTATTGTTGCCCGGAAACGGACTATTCCTTTAACGCTAAATCCGATGAAACAGAGTAACAACAAGCCACGAATTCGCCAGGCGGTTGGAGCCACCCGGCAATGTCGTAAACCACAGGCTTAAACTTCAACTTGGTAGCCTGTATCTTc                                                            >  2:860416/1‑151 (MQ=255)                                                                                           ttgttgCCCGGAAACGGACTATTCCTTTAACGCTAAATCCGATGAAACAGAGTAACAACAAGCCACGAATTCGCCAGGCGGTTGGAGCCACCCGGCAATGTCGTAAACCACAGGCTTAAACTTCAACTTGGTAGCCTGTATCTTCCAgtg                                                       >  1:222446/1‑150 (MQ=255)                                                                                            tgttgCCCGGAAACGGACTATTCCTTTAACGCTAAATCCGATGAAACAGAGTAACAACAAGCCACGAATTCGCCAGGCGGTTGGAGCCACCCGGCAATGTCGTAAACCACAGGCTTAAACTTCAACTTGGTAGCCTGTATCTTCCAgtgt                                                      <  1:209937/150‑1 (MQ=255)                                                                                                gCCCGGAAACGGACTATTCCTTTAACGCTAAATCCGATGAAACAGAGTAACAACAAGCCACGAATTCGCCAGGCGGTTGGAGCCACCCGGCAATGTCGTAAACCACAGGCTTAAACTTCAACTTGGTAGCCTGTATCTTCCAGTGTGGGAt                                                 <  1:75478/151‑1 (MQ=255)                                                                                                     gAAACGGACTATTCCTTTAACGCTAAATCCGATGAAACAGAGTAACAACAAGCCACGAATTCGCCAGGCGGTTGGAGCCACCCGGCAATGTCGTAAACCACAGGCTTAAACTTCAACTTGGTAGCCTGTATCTTCCAGTGTGGGATTCATc                                            <  2:275108/151‑1 (MQ=255)                                                                                                       aaCGGACTATTCCTTTAACGCTAAATCCGATGAAACAGAGTAACAACAAGCCACGAATTCGCCAGGCGGTTGGAGCCACCCGGCAATGTCGTAAACCACAGGCTTAAACTTCAACTTGGTAGCCTGTATCTTCCAGTGTGGGATTCATcgc                                          >  2:27985/1‑151 (MQ=255)                                                                                                        aCGGACTATTCCTTTAACGCTAAATCCGATGAAACAGAGTAACAACAAGCCACGAATTCGCCAGGCGGTTGGAGCCACCCGGCAATGTCGTAAACCACAGGCTTAAACTTCAACTTGGTAGCCTGTATCTTCCAGTGTGGGATTCATcgcc                                         >  1:442225/1‑151 (MQ=255)                                                                                                          ggACTATTCCTTTAACGCTAAATCCGATGAAACAGAGTAACAACAAGCCACGAATTCGCCAGGCGGTTGGAGCCACCCGGCAATGTCGTAAACCACAGGCTTAAACTTCAACTTGGTAGCCTGTATCTTCCAGTGTGGGATTCATcgccgc                                       >  1:889808/1‑151 (MQ=255)                                                                                                          ggACTATTCCTTTAACGCTAAATCCGATGAAACAGAGTAACAACAAGCCACGAATTCGCCAGGCGGTTGGAGCCACCCGGCAATGTCGTAAACCACAGGCTTAAACTTCAACTTGGTAGCCTGTATCTTCCAGTGTGGGATTCATcgccgc                                       <  1:876169/151‑1 (MQ=255)                                                                                                               aTTCCTTTAACGCTAAATCCGATGAAACAGAGTAACAACAAGCCACGAATTCGCCAGGCGGTTGGAGCCACCCGGCAATGTCGTAAACCACAGGCTTAAACTTCAACTTGGTAGCCTGTATCTTCCAGTGTGGGATTCATCGCCGCGGCAc                                  <  1:740439/151‑1 (MQ=255)                                                                                                               aTTCCTTTAACGCTAAATCCGATGAAACAGAGTAACAACAAGCCACGAATTCGCCAGGCGGTTGGAGCCACCCGGCAATGTCGTAAACCACAGGCTTAAACTTCAACTTGGTAGCCTGTATCTTCCAGTGTGGGATTCATCGCCGCGGCAc                                  >  2:931869/1‑151 (MQ=255)                                                                                                                 tCCTTTAACGCTAAATCCGATGAAACAGAGTAACAACAAGCCACGAATTCGCCAGGCGGTTGGAGCCACCCGGCAATGTCGTAAACCACAGGCTTAAACTTCAACTTGGTAGCCTGTATCTTCCAGTGTGGGATTCATCGCCGCGGCACg                                 <  1:183733/150‑1 (MQ=255)                                                                                                                      tAACGCTAAATCCGATGAAACAGAGTAACAACAAGCCACGAATTCGCCAGGCGGTTGGAGCCACCCGGCAATGTCGTAAACCACAGGCTTAAACTTCAACTTGGTAGCCTGTATCTTCCAGTGTGGGATTCATCGCCGCGGCACGAGcc                             >  1:80886/1‑149 (MQ=255)                                                                                                                       aaCGCTAAATCCGATGAAACAGAGTAACAACAAGCCACGAATTCGCCAGGCGGTTGGAGCCACCCGGCAATGTCGTAAACCACAGGCTTAAACTTCAACTTGGTAGCCTGTATCTTCCAGTGTGGGATTCATCGCCGCGGCACGAGCCAg                           <  2:324261/150‑1 (MQ=255)                                                                                                                        aCGCTAAATCCGATGAAACAGAGTAACAACAAGCCACGAATTCGCCAGGCGGTTGGAGCCACCCGGCAATGTCGTAAACCACAGGCTTAAACTTCAACTTGGTAGCCTGTATCTTCCAGTGTGGGATTCATCGCCGCGGCACGAGCCAGtt                         >  2:526521/1‑151 (MQ=255)                                                                                                                         cgatAAATCCGATGAAACAGAGTAACAACAAGCCACGAATTCGCCAGGCGGTTGGAGCCACCCGGCAATGTCGTAAACCACAGGCTTAAACTTCAACTTGGTAGCCTGTATCTTCCAGTGTGGGATTCATCGCCGCGGCACGAGCCAGTtc                        >  1:587425/4‑151 (MQ=255)                                                                                                                             aaaTCCGATGAAACAGAGTAACAACAAGCCACGAATTCGCCAGGCGGTTGGAGCCACCCGGCAATGTCGTAAACCACAGGCTTAAACTTCAACTTGGTAGCCTGTATCTTCCAGTGTGGGATTCATCGCCGCGGCACGAGCCAGTtcatc                     <  1:14672/150‑1 (MQ=255)                                                                                                                                tCCGATGAAACAGAGTAACAACAAGCCACGAATTCGCCAGGCGGTTGGAGCCACCCGGCAATGTCGTAAACCACAGGCTTAAACTTCAACTTGGTAGCCTGTATCTTCCAGTGTGGGATTCATCGCCGCGGCACGAGCCAGTTCATcac                   >  1:846123/1‑149 (MQ=255)                                                                                                                                      gAAACAGAGTAACAACAAGCCCCGAATTCGCCAGGCGGTTGGAGCCACCCGGCAATGTCGTAAACCACAGGCTTAAACTTCAACTTGGTAGCCTGTATCTTCCAGTGTGGGATTCATCGCCGCGGCACGAGCCAGTTCATCACAGcgttc            <  2:446749/150‑1 (MQ=255)                                                                                                                                      gAAACAGAGTAACAACAAGCCACGAATTCGCCAGGCGGTTGGAGCCACCCGGCAATGTCGTAAACCACAGGCTTAAACTTCAACTTGGTAGCCTGTATCTTCCAGTGTGGGATTCATCGCCGCGGCACGAGCCAGTTCATCACAGcgttcg           >  2:515632/1‑151 (MQ=255)                                                                                                                                        aaCAGAGTAACAACAAGCCACGAATTCGCCAGGCGGTTGGAGCCACCCGGCAATGTCGTAAACCACAGGCTTAAACTTCAACTTGGTAGCCTGTATCTTCCAGTGTGGGATTCATCGCCGCGGCACGAGCCAGTTCATCACAGcgttcgtt         >  2:384211/1‑151 (MQ=255)                                                                                                                                             agTAACAACAAGCCACGAATTCGCCAGGCGGTTGGAGCCACCCGGCAATGTCGTAAACCACAGGCTTAAACTTCAACTTGGTAGCCTGTATCTTCCAGTGTGGGATTCATCGCCGCGGCACGAGCCAGTTCATCACAGCGTTCGTTTTCCg    <  2:354341/151‑1 (MQ=255)                                                                                                                                               tAACAACAAGCCACGAATTCGCCAGACGGTTGGAGCCACCCGGCAATGTCGTAAACCACAGGCTTAAACTTCAACTTGGTAGCCTGTATCTTCCAGTGTGGGATTCATCGCCGCGGCACGAGCCAGTTCATCACAGCGTTCGTTTTCCggg  >  1:736812/1‑151 (MQ=255)                                                                                                                                                  |                                                                                                                                                    ACGCCAGCCGTTTCCACGTTCTCCCGTGGAACAAACACGGAGGAAAACTATTGAATGCGCATATTCCTGCGCTTGGTTGCTTACAACTTATTGTTGCCCGGAAACGGACTATTCCTTTAACGCTAAATCCGATGAAACAGAGTAAAAACAAGCCACGAATTCGCCAGGCGGTTGGAGCCACCCGGCAATGTCGTAAACCACAGGCTTAAACTTCAACTTGGTAGCCTGTATCTTCCAGTGTGGGATTCATCGCCGCGGCACGAGCCAGTTCATCACAGCGTTCGTTTTCCGGG  >  NC_000913/235330‑235622

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑