Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,220,666 T→G K608Q (AAA→CAA)  arpA ← ankyrin repeat protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,220,6660TG96.7% 201.2 / 0.6 60K608Q (AAA→CAA) arpAankyrin repeat protein
Reads supporting (aligned to +/- strand):  ref base T (0/2);  new base G (34/24);  total (34/26)
Fisher's exact test for biased strand distribution p-value = 1.84e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.32e-01

CAGCGGTAAATCCAAACAGGCGTGCATGGTCAGATAAAGCAAGATAAACCGTCTCTACAACATTTTGTTGTTTATGCTGTATCGCTGAAAACAAACCGGGATATTCATTAGAATTATTTGCCAGGAGGAGGGGCTTCATATTTTTTTTATCGAATTTAAACGTATTAAACAGAGTGGGTAATGCGTTAAAAATAGTATTAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTG  >  NC_000913/4220519‑4220812
                                                                                                                                                   |                                                                                                                                                  
cAGCGGTAAATCCAAACAGGCGTGCATGGTCAGATAAAGCAAGATAAACCGTCTCTACAACATTTTGTTGTTTATGCTGTATCGCTGAAAACAAACCGGGATATTCATTAGAATTATTTGCCAGGAGGAGGGGCTTCATATTTTTTTGATc                                                                                                                                                 <  1:411472/151‑1 (MQ=255)
 aGCGGTAAATCCAAACAGGCGTGCATGGTCAGATAAAGCAAGATAAACCGTCTCTACAACATTTTGTTGTTTATGCTGTATCGCTGAAAACAAACCGGGATATTCATTAGAATTATTTGCCAGGAGGAGGGGCTTCATATTTTTTTGATCg                                                                                                                                                <  1:319398/151‑1 (MQ=255)
 aGCGGTAAATCCAAACAGGCGTGCATGGTCAGATAAAGCAAGATAAACCGTCTCTACAACATTTTGTTGTTTATGCTGTATCGCTGAAAACAAACCGGGATATTCATTAGAATTATTTGCCAGGAGGAGGGGCTTCATATTTTTTTGATCg                                                                                                                                                >  1:875710/1‑151 (MQ=255)
  gCGGTAAATCCAAACAGGCGTGCATGGTCAGATAAAGCAAGATAAACCGTCTCTACAACATTTTGTTGTTTATGCTGTATCGCTGAAAACAAACCGGGATATTCATTAGAATTATTTGCCAGGAGGAGGGGCTTCATATTTTTTTGATCg                                                                                                                                                >  2:621586/1‑150 (MQ=255)
  gCGGTAAATCCAAACAGGCGTGCATGGTCAGATAAAGCAAGATAAACCGTCTCTACAACATTTTGTTGTTTATGCTGTATCGCTGAAAACAAACCGGGATATTCATTAGAATTATTTGCCAGGAGGAGGGGCTTCATATTTTTTTGATCGa                                                                                                                                               <  2:516970/151‑1 (MQ=255)
   cGGTAAATCCAAACAGGCGTGCATGGTCAGATAAAGCAAGATAAACCGTCTCTACAACATTTTGTTGTTTATGCTGTATCGCTGAAAACAAACCGGGATATTCATTAGAATTATTTGCCAGGAGGAGGGGCTTCATATTTTTTTGATCGa                                                                                                                                               >  2:216871/1‑150 (MQ=255)
   cGGTAAATCCAAACAGGCGTGCATGGTCAGATAAAGCAAGATAAACCGTCTCTACAACATTTTGTTGTTTATGCTGTATCGCTGAAAACAAACCGGGATATTCATTAGAATTATTTGCCAGGAGGAGGGGCTTCATATTTTTTTGATCGa                                                                                                                                               >  2:169962/1‑150 (MQ=255)
       aaaTCCAAACAGGCGTGCATGGTCAGATAAAGCAAGATAAACCGTCTCTACAACATTTTGTTGTTTATGCTGTATCGCTGAAAACAAACCGGGATATTCATTAGAATTATTTGCCAGGAGGAGGGGCTTCATATTTTTTTGATCGAAttt                                                                                                                                           >  2:606427/1‑150 (MQ=255)
           ccAAACAGGCGTGCATGGTCAGATAAAGCAAGATAAACCGTCTCTACAACATTTTGTTGTTTATGCTGTATCGCTGAAAACAAACCGGGATATTCATTAGAATTATTTGCCAGGAGGAGGGGCTTCATATTTTTTTGATCGAATTTAAACg                                                                                                                                      <  2:651177/151‑1 (MQ=255)
            cAAACAGGCGTGCATGGTCAGATAAAGCAAGATAAACCGTCTCTACAACATTTTGTTGTTTATGCTGTATCGCTGAAAACAAACCGGGATATTCATTAGAATTATTTGCCAGGAGGAGGGGCTTCATATTTTTTTGATCGAATTTAAACGt                                                                                                                                     >  1:899133/1‑151 (MQ=255)
                 aGGCGTGCATGGTCAGATAAAGCAAGATAAACCGTCTCTACAACATTTTGTTGTTTATGCTGTATCGCTGAAAACAAACCGGGATATTCATTAGAATTATTTGCCAGGAGGAGGGGCTTCATATTTTTTTGATCGAATTTAAACGTATTa                                                                                                                                 >  2:10779/1‑150 (MQ=255)
                  ggCGTGCATGGTCAGATAAAGCAAGATAAACCGTCTCTACAACATTTTGTTGTTTATGCTGTATCGCTGAAAACAAACCGGGATATTCATTAGAATTATTTGCCAGGAGGAGGGGCTTCATATTTTTTTGATCGAATTTAAACGTATTaa                                                                                                                                >  2:929926/1‑150 (MQ=255)
                       gCATGGTCAGATAAAGCAAGATAAACCGTCTCTACAACATTTTGTTGTTTATGCTGTATCGCTGAAAACAAACCGGGATATTCATTAGAATTATTTGCCAGGAGGAGGGGCTTCATATTTTTTTGATCGAATTTAAACGTATTAAACaga                                                                                                                           <  1:232009/150‑1 (MQ=255)
                         aTGGTCAGATAAAGCAAGATAAACCGTCTCTACAACATTTTGTTGTTTATGCTGTATCGCTGAAAACAAACCGGGATATTCATTAGAATTATTTGCCAGGAGGAGGGGCTTCATATTTTTTTGATCGAATTTAAACGTATTAAACAGAGTg                                                                                                                        >  1:186005/1‑151 (MQ=255)
                         aTGGTCAGATAAAGCAAGATAAACCGTCTCTACAACATTTTGTTGTTTATGCTGTATCGCTGAAAACAAACCGGGATATTCATTAGAATTATTTGCCAGGAGGAGGGGCTTCATATTTTTTTGATCGAATTTAAACGTATTAAACAGAGTg                                                                                                                        >  2:34961/1‑151 (MQ=255)
                            gTCAGATAAAGCAAGATAAACCGTCTCTACAACATTTTGTTGTTTATGCTGTATCGCTGAAAACAAACCGGGATATTCATTAGAATTATTTGCCAGGAGGAGGGGCTTCATATTTTTTTGATCGAATTTAAACGTATTAAACAGAGTGGGt                                                                                                                     <  2:35458/151‑1 (MQ=255)
                             tCAGATAAAGCAAGATAAACCGTCTCTACAACATTTTGTTGTTTATGCTGTATCGCTGAAAACAAACCGGGATATTCATTAGAATTATTTGCCAGGAGGAGGGGCTTCATATTTTTTTGATCGAATTTAAACGTATTAAACAGAGTGGGt                                                                                                                     >  1:720903/1‑150 (MQ=255)
                                gATAAAGCAAGATAAACCGTCTCTACAACATTTTGTTGTTTATGCTGTATCGCTGAAAACAAACCGGGATATTCATTAGAATTATTTGCCAGGAGGAGGGGCTTCATATTTTTTTGATCGAATTTAAACGTATTAAACAGAGTGGGTAATg                                                                                                                 <  2:478557/151‑1 (MQ=255)
                                 aTAAAGCAAGATAAACCGTCTCTACAACATTTTGTTGTTTATGCTGTATCGCTGAAAACAAACCGGGATATTCATTAGAATTATTTGCCAGGAGGAGGGGCTTCATATTTTTTTGATCGAATTTAAACGTATTAAACAGAGTGGGTAATGc                                                                                                                <  1:862031/151‑1 (MQ=255)
                                    aaGCAAGATAAACCGTCTCTACAACATTTTGTTGTTTATGCTGTATCGCTGAAAACAAACCGGGATATTCATTAGAATTATTTGCCAGGAGGAGGGGCTTCATATTTTTTTGATCGAATTTAAACGTATTAAACAGAGTGGGTAATGCGtt                                                                                                             <  2:215516/151‑1 (MQ=255)
                                       cAAGATAAACCGTCTCTACAACATTTTGTTGTTTATGCTGTATCGCTGAAAACAAACCGGGATATTCATTAGAATTATTTGCCAGGAGGAGGGGCTTCATATTTTTTTGATCGAATTTAAACGTATTAAACAGAGTGGGTAATGCGTTaa                                                                                                           >  1:737002/1‑150 (MQ=255)
                                        aaGATAAACCGTCTCTACAACATTTTGTTGTTTATGCTGTATCGCTGAAAACAAACCGGGATATTCATTAGAATTATTTGCCAGGAGGAGGGGCTTCATATTTTTTTGATCGAATTTAAACGTATTAAACAGAGTGGGTAATGCGTTaaaa                                                                                                         <  2:394551/151‑1 (MQ=255)
                                              aaCCGTCTCTACAACATTTTGTTGTTTATGCTGTATCGCTGAAAACAAACCGGGATATTCATTAGAATTATTTGCCAGGAGGAGGGGCTTCATATTTTTTTGATCGAATTTAAACGTATTAAACAGAGTGGGTAATGCGTTAAAAATAGTa                                                                                                   <  2:403245/151‑1 (MQ=255)
                                               aCCGTCTCTACAACATTTTGTTGTTTATGCTGTATCGCTGAAAACAAACCGGGATATTCATTAGAATTATTTGCCAGGAGGAGGGGCTTCATATTTTTTTGATCGAATTTAAACGTATTAAACAGAGTGGGTAATGCGTTAAAAATAGTAt                                                                                                  >  1:299958/1‑151 (MQ=255)
                                                ccGTCTCTACAACATTTTGTTGTTTATGCTGTATCGCTGAAAACAAACCGGGATATTCATTAGAATTATTTGCCAGGAGGAGGGGCTTCATATTTTTTTGATCGAATTTAAACGTATTAAACAGAGTGGGTAATGCGTTAAAAATAGTAtt                                                                                                 <  2:336052/151‑1 (MQ=255)
                                                ccGTCTCTACAACATTTTGTTGTTTATGCTGTATCGCTGAAAACAAACCGGGATATTCATTAGAATTATTTGCCAGGAGGAGGGGCTTCATATTTTTTTGATCGAATTTAAACGTATTAAACAGAGTGGGTAATGCGTTAAAAATAGTAtt                                                                                                 >  2:197181/1‑151 (MQ=255)
                                                 cGTCTTTACAACATTTTGGTGTTTATGCTGTATCGCTGAAATCAATCCGTGTTATTCAGTAGAATTATTTGCCAGGAGGGGGGGCTTCATATTTTTTTGCACGAAGTTAATGTTAGTAAACAGGGTGGTTAATGTGTTAAAAATAGAAttg                                                                                                <  1:907507/151‑2 (MQ=255)
                                                 cGTCTCTACAACATTTTGTTGTTTATGCTGTATCGCTGAAAACAAACCGGGATATTCATTAGAATTATTTGCCAGGAGGAGGGGCTTCATATTTTTTTGATCGAATTTAAACGTATTAAACAGAGTGGGTAATGCGTTAAAAATAGTAtt                                                                                                 >  1:246768/1‑150 (MQ=255)
                                                 cGTCTCTACAACATTTTGTTGTTTATGCTGTATCGCTGAAAACAAACCGGGATATTCATTAGAATTATTTGCCAGGAGGAGGGGCTTCATATTTTTTTGATCGAATTTAAACGTATTAAACAGAGTGGGTAATGCGTTAAAAATAGTATta                                                                                                <  1:907492/151‑1 (MQ=255)
                                                  gTCTCTACAACATTTTGTTGTTTATGCTGTATCGCTGAAAACAAACCGGGATATTCATTAGAATTATTTGCCAGGAGGAGGGGCTTCATATTTTTTTGATCGAATTTAAACGTATTAAACAGAGTGGGTAATGCGTTAAAAATAGTATta                                                                                                >  1:578801/1‑150 (MQ=255)
                                                        acaacaTTTTGTTGTTTATGCTGTATCGCTGAAAACAAACCGGGATATTCATTAGAATTATTTGCCAGGAGGAGGGGCTTCATATTTTTTTTATCGAATTTAAACGTATTAAACAGAGTGGGTAATGCGTTAAAAATAGTATTAATAACg                                                                                          <  2:358826/150‑1 (MQ=255)
                                                            caTTTTGTTGTTTATGCTGTATCGCTGAAAACAAACCGGGATATTCATTAGAATTATTTGCCAGGAGGAGGGGCTTCATATTTTTTTGATCGAATTTAAACGTATTAAACAGAGTGGGTAATGCGTTAAAAATAGTATTAATAACGTTCAt                                                                                     >  2:797022/1‑151 (MQ=255)
                                                             aTTTTGTTGTTTATGCTGTATCGCTGAAAACAAACCGGGATATTCATTAGAATTATTTGCCAGGAGGAGGGGCTTCATATTTTTTTGATCGAATTTAAACGTATTAAACAGAGTGGGTAATGCGTTAAAAATAGTATTAATAACGTTCAtg                                                                                    >  2:38060/1‑151 (MQ=255)
                                                              ttttGTTGTTTATGCTGTATCGCTGAAAACAAACCGGGATATTCATTAGAATTATTTGCCAGGAGGAGGGGCTTCATATTTTTTTGATCGAATTTAAACGTATTAAACAGAGTGGGTAATGCGTTAAAAATAGTATTAATAACGTTCAtgt                                                                                   >  2:142113/1‑151 (MQ=255)
                                                               tttGTTGTTTATGCTGTATCGCTGAAAACAAACCGGGATATTCATTAGAATTATTTGCCAGGAGGAGGGGCTTCATATTTTTTTGATCGAATTTAAACGTATTAAACAGAGTGGGTAATGCGTTAAAAATAGTATTAATAACGTTCAtgtg                                                                                  >  2:877956/1‑151 (MQ=255)
                                                               tttGTTGTTTATGCTGTATCGCTGAAAACAAACCGGGATATTCATTAGAATTATTTGCCAGGAGGAGGGGCTTCATATTTTTTTGATCGAATTTAAACGTATTAAACAGAGTGGGTAATGCGTTAAAAATAGTATTAATAACGTTCAtgtg                                                                                  >  1:589069/1‑151 (MQ=255)
                                                                  gttgttTATGCTGTATCGCTGAAAACAAACCGGGATATTCATTAGAATTATTTGCCAGGAGGAGGGGCTTCATATTTTTTTGATCGAATTTAAACGTATTAAACAGAGTGGGTAATGCGTTAAAAATAGTATTAATAACGTTCATGTGTcc                                                                               >  2:593300/1‑151 (MQ=255)
                                                                     gttTATGCTGTATCGCTGAAAACAAACCGGGATATTCATTAGAATTATTTGCCAGGAGGAGGGGCTTCATATTTTTTTGATCGAATTTAAACGTATTAAACAGAGTGGGTAATGCGTTAAAAATAGTATTAATAACGTTCATGTGTCcgcg                                                                            >  2:826332/1‑151 (MQ=255)
                                                                           gCTGTATCGCTGAAAACAAACCGGGATATTCATTAGAATTATTTGCCAGGAGGAGGGGCTTCATATTTTTTTGATCGAATTTAAACGTATTAAACAGAGTGGGTAATGCGTTAAAAATAGTATTAATAACGTTCATGTGTCCGCGCTGCAt                                                                      >  2:236301/1‑151 (MQ=255)
                                                                                aTCGCTGAAAACAAACCGGGATATTCATTAGAATTATTTGCCAGGAGGAGGGGCTTCATATTTTTTTGATCGAATTTAAACGTATTAAACAGAGAGGGTAATGCGTTAAAAATAGTATTAATAACGTTCATGTGTCCGCGCTGCATGGcc                                                                  >  2:100839/1‑150 (MQ=255)
                                                                                  cGCTGAAAACAAACCGGGATATTCATTAGAATTATTTGCCAGGAGGAGGGGCTTCATATTTTTTTTATCGAATTTAAACGTATTAAACAGAGTGGGTAATGCGTTAAAAATAGTATTAATAACGTTCATGTGTCCGCGCTGCATGGCCAt                                                                <  2:218801/150‑1 (MQ=255)
                                                                                   gCTGAAAACAAACCGGGATATTCATTAGAATTATTTGCCAGGAGGAGGGGCTTCATATTTTTTTGATCGAATTTAAACGTATTAAACAGAGTGGGTAATGCGTTAAAAATAGTATTAATAACGTTCATGTGTCCGCGCTGCATGGCCATa                                                               >  1:39218/1‑150 (MQ=255)
                                                                                           caaacCGGGATATTCATTAGAATTATTTGCCAGGAGGAGGGGCTTCATATTTTTTTGATCGAATTTAAACGTATTAAACAGAGTGGGTAATGCGTTAAAAATAGTATTAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAAccc                                                      <  2:236387/151‑1 (MQ=255)
                                                                                                cGGGATATTCATTAGAATTATTTGCCAGGAGGAGGGGCTTCATATTTTTTTGATCGAATTTAAACGTATTAAACAGAGTGGGTAATGCGTTAAAAATAGTATTAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTc                                                 <  1:767255/151‑1 (MQ=255)
                                                                                                   gATATTCATTAGAATTATTTGCCAGGAGGAGGGGCTTCATATTTTTTTGATCGAATTTAAACGTATTAAACAGAGTGGGTAATGCGTTAAAAATAGTATTAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTcgcg                                              >  2:296735/1‑151 (MQ=255)
                                                                                                        tCATTAGAATTATTTGCCAGGAGGAGGGGCTTCATATTTTTTTGATCGAATTTAAACGTATTAAACAGAGTGGGTAATGCGTTAAAAATAGTATTAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCa                                          >  1:710924/1‑150 (MQ=255)
                                                                                                         cATTAGAATTATTTGCCAGGAGGAGGGGCTTCATATTTTTTTGATCGAATTTAAACGTATTAAACAGAGTGGGTAATGCGTTAAAAATAGTATTAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAg                                        <  2:178838/151‑1 (MQ=255)
                                                                                                              gAATTATTTGCCAGGAGGAGGGGCTTCATATTTTTTTGATCGAATTTAAACGTATTAAACAGAGTGGGTAATGCGTTAAAAATAGTATTAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACttt                                   <  1:537547/151‑1 (MQ=255)
                                                                                                                attattTGCCAGGAGGAGGGGCTTCATATTTTTTTGATCGAATTTAAACGTATTAAACAGAGTGGGTAATGCGTTAAAAATAGTATTAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACtttt                                  >  1:251021/1‑150 (MQ=255)
                                                                                                                attattTGCCAGGAGGAGGGGCTTCATATTTTTTTGATCGAATTTAAACGTATTAAACAGAGTGGGTAATGCGTTAAAAATAGTATTAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTa                                 <  2:85741/151‑1 (MQ=255)
                                                                                                                     ttGCCAGGAGGAGGGGCTTCATATTTTTTTGATCGAATTTAAACGTATTAAACAGAGTGGGTAATGCGTTAAAAATAGTATTAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGt                            >  1:129551/1‑151 (MQ=255)
                                                                                                                       gCCAGGAGGAGGGGCTTCATATTTTTTTGATCGAATTTAAACGTATTAAACAGAGTGGGTAATGCGTTAAAAATAGTATTAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTc                           >  2:111461/1‑150 (MQ=255)
                                                                                                                       gCCAGGAGGAGGGGCTTCATATTTTTTTGATCGAATTTAAACGTATTAAACAGAGTGGGTAATGCGTTAAAAATAGTATTAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCa                          <  1:902895/151‑1 (MQ=255)
                                                                                                                        ccAGGAGGAGGGGCTTCATATTTTTTTGATCGAATTTAAACGTATTAAACAGAGTGGGTAATGCGTTAAAAATAGTATTAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTc                           >  1:639157/1‑149 (MQ=255)
                                                                                                                         cAGGAGGAGGGGATTCATATTTTTTTGATCGAATTTAAACGTATTAAACAGAGTGGGTAATGCGTTAAAAATAGTATTAATAACGTTCATGTGTCCGCGCTGCATTGCCATTAACAAACTCGTCTCGCGCGCAACAGTTTTAGCgg                             >  1:401035/1‑146 (MQ=255)
                                                                                                                           ggaggaggGGCTTCATATTTTTTTGATCGAATTTAAACGTATTAAACAGAGTGGGTAATGCGTTAAAAATAGTATTAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCagaag                      <  2:793256/151‑1 (MQ=255)
                                                                                                                            gaggaggGGCTTCATATTTTTTTGATCGAATTTAAACGTATTAAACAGAGTGGGTAATGCGTTAAAAATAGTATTAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCagaaga                     <  2:512330/151‑1 (MQ=255)
                                                                                                                             aggaggGGCTTCATATTTTTTTGATCGAATTTAAACGTATTAAACAGAGTGGGTAATGCGTTAAAAATAGTATTAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGAt                    <  2:556031/151‑1 (MQ=255)
                                                                                                                             aggaggGGCTTCATATTTTTTTGATCGAATTTAAACGTATTAAACAGAGTGGGTAATGCGTTAAAAATAGTATTAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGAt                    <  2:209588/151‑1 (MQ=255)
                                                                                                                                       tCATATTTTTTTGATCGAATTTAAACGTATTAAACAGAGTGGGTAATGCGTTAAAAATAGTATTAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATc          >  2:262903/1‑151 (MQ=255)
                                                                                                                                                tttgATCGAATTTAAACGTATTAAACAGAGTGGGTAATGCGTTAAAAATAGTATTAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTg  >  1:215460/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CAGCGGTAAATCCAAACAGGCGTGCATGGTCAGATAAAGCAAGATAAACCGTCTCTACAACATTTTGTTGTTTATGCTGTATCGCTGAAAACAAACCGGGATATTCATTAGAATTATTTGCCAGGAGGAGGGGCTTCATATTTTTTTTATCGAATTTAAACGTATTAAACAGAGTGGGTAATGCGTTAAAAATAGTATTAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTG  >  NC_000913/4220519‑4220812

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑