Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,268,001 T→G A296A (GCT→GCG)  tyrB → tyrosine aminotransferase, tyrosine‑repressible, PLP‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,268,0010TG98.2% 187.2 / ‑4.1 54A296A (GCT→GCG) tyrBtyrosine aminotransferase, tyrosine‑repressible, PLP‑dependent
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base G (24/29);  total (24/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.19e-01

TTTCTCCCTTTACGGCGAGCGCGTCGGCGGACTTTCTGTTATGTGTGAAGATGCCGAAGCCGCTGGCCGCGTACTGGGGCAATTGAAAGCAACAGTTCGCCGCAACTACTCCAGCCCGCCGAATTTTGGTGCGCAGGTGGTGGCTGCAGTGCTGAATGACGAGGCATTGAAAGCCAGCTGGCTGGCGGAAGTAGAAGAGATGCGTACTCGCATTCTGGCAATGCGTCAGGAATTGGTGAAGGTATTAAGCACAGAGATGCCAGAACGCAATTTCGATTATCTGCTTAAT  >  NC_000913/4267857‑4268145
                                                                                                                                                |                                                                                                                                                
tttCTCCCTTTACGGCGAGCGCGTCGGCGGACTTTCTGTTATGTGTGAAGATGCCGAAGCCGCTGGCCGCGTACTGGGGCAATTGAAAGCAACAGTTCGCCGCAACTACTCCAGCCCGCCGAATTTTGGTGCGCAGGTGGTGGCGGCAGt                                                                                                                                             <  2:677229/150‑1 (MQ=255)
 ttCTCCCTTTACGGCGAGCGCGTCGGCGGACTTTCTGTTATGTGTGAAGATGCCGAAGCCGCTGGCCGCGTACTGGGGCAATTGAAAGCAACAGTTCGCCGCAACTACTCCAGCCCGCCGAATTTTGGTGCGCAGGTGGTGGCGGCAGTg                                                                                                                                            <  1:210007/150‑1 (MQ=255)
   ctcCCTTTACGGCGAGCGCGTCGGCGGACTTTCTGTTATGTGTGAAGATGCCGAAGCCGCTGGCCGCGTACTGGGGCAATTGAAAGCAACAGTTCGCCGCAACTACTCCAGCCCGCCGAATTTTGGTGCGCAGGTGGTGGCGGCAGTGCTg                                                                                                                                         >  2:365300/1‑151 (MQ=255)
       cTTTACGGCGAGCGCGTCGGCGGACTTTCTGTTATGTGTGAAGATGCCGAAGCCGCTGGCCGCGTACTGGGGCAATTGAAAGCAACAGTTCGCCGCAACTACTCCAGCCCGCCGAATTTTGGTGCGCAGGTGGTGGCGGCAGTGCTGAATg                                                                                                                                     >  1:901573/1‑151 (MQ=255)
         ttACGGCGAGCGCGTCGGCGGACTTTCTGTTATGTGTGAAGATGCCGAAGCCGCTGGCCGCGTACTGGGGCAATTGAAAGCAACAGTTCGCCGCAACTACTCCAGCCCGCCGAATTTTGGTGCGCAGGTGGTGGCGGCAGTGCTGAATg                                                                                                                                     <  1:860336/149‑1 (MQ=255)
                     cgTCGGCGGACTTTCTGTTATGTGTGAAGATGCCGAAGCCGCTGGCCGCGTACTGGGGCAATTGAAAGCAACAGTTCGCCGCAACTACTCCAGCCCGCCGAATTTTGGTGCGCAGGTGGTGGCGGCAGTGCTGAATGACGAGGCATTGaaa                                                                                                                       <  1:376034/151‑1 (MQ=255)
                     cgTCGGCGGACTTTCTGTTATGTGTGAAGATGCCGAAGCCGCTGGCCGCGTACTGGGGCAATTGAAAGCAACAGTTCGCCGCAACTACTCCAGCCCGCCGAATTTTGGTGCGCAGGTGGTGGCGGCAGTGCTGAATGACGAGGCATTGaaa                                                                                                                       <  2:605589/151‑1 (MQ=255)
                      gTCGGCGGACTTTCTGTTATGTGTGAAGATGCCGAAGCCGCTGGCCGCGTACTGGGGCAATTGAAAGCAACAGTTCGCCGCAACTACTCCAGCCCGCCGAATTTTGGTGCGCAGGTGGTGGCGGCAGTGCTGAATGACGAGGCATTGaa                                                                                                                        >  2:869227/1‑149 (MQ=255)
                        cggcggACTTTCTGTTATGTGTGAAGATGCCGAAGCCGCTGGCCGCGTACTGGGGCAATTGAAAGCAACAGTTCGCCGCAACTACTCCAGCCCGCCGAATTTTGGTGCGCAGGTGGTGGCGGCAGTGCTGAATGACGAGGCATTGAAAGcc                                                                                                                    <  2:767185/151‑1 (MQ=255)
                         ggcggACTTTCTGTTATGTGTGAAGATGCCGAAGCCGCTGGCCGCGTACTGGGGCAATTGAAAGCAACAGTTCGCCGCAACTACTCCAGCCCGCCGAATTTTGGTGCGCAGGTGGTGGCGGCAGTGCTGAATGACGAGGCATTGAAAGCCa                                                                                                                   <  1:489198/151‑1 (MQ=255)
                          gcggACTTTCTGTTATGTGTGAAGATGCCGAAGCCGCTGGCCGCGTACTGGGGCAATTGAAAGCAACAGTTCGCCGCAACTACTCCAGCCCGCCGAATTTTGGTGCGCAGGTGGTGGCGGCAGTGCTGAATGACGAGGCATTGAAAGCCAg                                                                                                                  <  2:145524/151‑1 (MQ=255)
                            ggACTTTCTGTTATGTGTGAAGATGCCGAAGCCGCTGGCCGCGTACTGGGGCAATTGAAAGCAACAGTTCGCCGCAACTACTCAAGCCCGCCGAATTTTGGTGCGCAGGTGGCGGAGGCAGTGCTGAATGACGAGGCATTGAAAGCCAg                                                                                                                  >  2:451182/1‑149 (MQ=255)
                                tttCTGTTATGTGTGAAGATGCCGAAGCCGCTGGCCGCGTACTGGGGCAATTGAAAGCAACAGTTCGCCGCAACTACTCCAGCCCGCCGAATTTTGGTGCGCAGGTGGTGGCGGCAGTGCTGAATGACGAGGCATTGAAAGCCAgctggct                                                                                                            >  1:496316/1‑151 (MQ=255)
                                  tCTGTTATGTGTGAAGATGCCGAAGCCGCTGGCCGCGTACTGGGGCAATTGAAAGCAACAGTTCGCCGCAACTACTCCAGCCCGCCGAATTTTGGTGCGCAGGTGGTGGCGGCAGTGCTGAATGACGAGGCATTGAAAGCCAGctggctgg                                                                                                          >  1:867070/1‑151 (MQ=255)
                                     gTTATGTGTGAAGATGCCGAAGCCGCTGGCCGCGTACTGGGGCAATTGAAAGCAACAGTTCGCCGCAACTACTCCAGCCCGCCGAATTTTGGTGCGCAGGTGGTGGCGGCAGTGCTGAATGACGAGGCATTGAAAGCCAGCtggctggc                                                                                                         <  1:162226/149‑1 (MQ=255)
                                      ttATGTGTGAAGATGCCGAAGCCGCTGGCCGCGTACTGGGGCAATTGAAAGCAACAGTTCGCCGCAACTACTCCAGCCCGCCGAATTTTGGTGCGCAGGTGGTGGCGGCAGTGCTGAATGACGAGGCATTGAAAGCCAGCTGGCTGGCgg                                                                                                       >  2:84485/1‑150 (MQ=255)
                                          gtgtgAAGATGCCGAAGCCGCTGGCCGCGTACTGGGGCAATTGAAAGCAACAGTTCGCCGCAACTACTCCAGCCCGCCGAATTTTGGTGCGCAGGTGGTGGCTGCAGTGCTGAATGACGAGGCATTGAAAGCCAGCTGGCTGGCGGAAg                                                                                                    <  1:211802/149‑1 (MQ=255)
                                                   tGCCGAAGCCGCTGGCCGCGTACTGGGGCAATTGAAAGCAACAGTTCGCCGCAACTACTCCAGCCCGCCGAATTTTGGTGCGCAGGTGGTGGCGGCAGTGCTGAATGACGAGGCATTGAAAGCCAGCTGGCTGGCGGAAGTAGAAGAGATg                                                                                         >  1:625323/1‑151 (MQ=255)
                                                    gCCGAAGCCGCTGGCCGCGTACTGGGGCAATTGAAAGCAACAGTTCGCCGCAACTACTCCAGCCCGCCGAATTTTGGTGCGCAGGTGGTGGCGGCAGTGCTGAATGACGAGGCATTGAAAGCCAGCTGGCTGGCGGAAGTAGAAGAGATGc                                                                                        <  2:65155/151‑1 (MQ=255)
                                                     ccGAAGCCGCTGGCCGCGTACTGGGGCAATTGAAAGCAACAGTTCGCCGCAACTACTCCAGCCCGCCGAATTTTGGTGCGCAGGTGGTGGCGGCAGTGCTGAATGACGAGGCATTGAAAGCCAGCTGGCTGGCGGAAGTAGAAGAGATGCg                                                                                       <  1:739372/151‑1 (MQ=255)
                                                               tGGCCGCGTACTGGGGCAATTGAAAGCAACAGTTCGCCGCAACTACTCCAGCCCGCCGAATTTTGGTGCGCAGGTGGTGGCGGCAGTGCTGAATGACGAGGCATTGAAAGCCAGCTGGCTGGCGGAAGTAGAAGAGATGCGTACTCGCAtt                                                                             >  2:332550/1‑151 (MQ=255)
                                                                   cgcgTACTGGGGCAATTGAAAGCAACAGTTCGCCGCAACTACTCCAGCCCGCCGAATTTTGGTGCGCAGGTGGTGGCGGCAGTGCTGAATGACGAGGCATTGAAAGCCAGCTGGCTGGCGGAAGTAGAAGAGATGCGTACTCGCATTCTgg                                                                         <  1:732600/151‑1 (MQ=255)
                                                                     cgTACTGGGGCAATTGAAAGCAACAGTTCGCCGCAACTACTCCAGCCCGCCGAATTTTGGTGCGCAGGTGGTGGCGGCAGTGCTGAATGACGAGGCATTGAAAGCCAGCTGGCTGGCGGAAGTAGAAGAGATGCGTACTCGCATTCTGGc                                                                        >  2:376575/1‑150 (MQ=255)
                                                                      gTACTGGGGCAATTGAAAGCAACAGTTCGCCGCAACTACTCCAGCCCGCCGAATTTTGGTGCGCAGGTGGTGGCGGCAGTGCTGAATGACGAGGCATTGAAAGCCAGCTGGCTGGCGGAAGTAGAAGAGATGCGTACTCGCATTCTGGCa                                                                       <  1:855131/150‑1 (MQ=255)
                                                                        aCTGGGGCAATTGAAAGCAACAGTTCGCCGCAACTACTCCAGCCCGCCGAATTTTGGTGCGCAGGTGGTGGCGGCAGTGCTGAATGACGAGGCATTGAAAGCCAGCTGGCTGGCGGAAGTAGAAGAGATGCGTACTCGCATTCTGGCAATg                                                                    <  2:401380/151‑1 (MQ=255)
                                                                            gggCAATTGAAAGCAACAGTTCGCCGCAACTACTCCAGCCCGCCGAATTTTGGTGCGCAGGTGGTGGCGGCAGTGCTGAATGACGAGGCATTGAAAGCCAGCTGGCTGGCGGAAGTAGAAGAGATGCGTACTCGCATTCTGGCAATGCGTc                                                                >  1:879716/1‑151 (MQ=255)
                                                                                aaTTGAAAGCAACAGTTCGCCGCAACTACTCCAGCCCGCCGAATTTTGGTGCGCAGGTGGTGGCGGCAGTGCTGAATGACGAGGCATTGAAAGCCAGCTGGCTGGCGGAAGTAGAAGAGATGCGTACTCGCATTCTGGCAATGCGTCAgg                                                             >  2:160876/1‑150 (MQ=255)
                                                                                   caataGCAACCGGTCACCGCACCTACTCCAGCCCGCCGAATTTTGGTGCGCAGGTGGTGGCGGCGGTGCTGAATGACGAGGCATTGAAAGACAGCTGGCTGGCGGAAGTAGAAGAGATGCGTACTCGCATTCTGGCCATGCGTCAGGAAtt                                                         >  1:426220/5‑151 (MQ=255)
                                                                                     aaaGCAACAGTTCGCCGCAACTACTCCAGCCCGCCGAATTTTGGTGCGCAGGTGGTGGCGGCAGTGCTGAATGACGAGGCATTGAAAGCCAGCTGGCTGGCGGAAGTAGAAGAGATGCGTACTCGCATTCTGGCAATGCGTCAGGAATTgg                                                       <  2:94101/151‑1 (MQ=255)
                                                                                      aaGCAACAGTTCGCCGCAACTACTCCAGCCCGCCGAATTTTGGTGCGCAGGTGGTGGCGGCAGTGCTGAATGACGAGGCATTGAAAGCCAGCTGGCTGGCGGAAGTAGAAGAGATGCGTACTCGCATTCTGGCAATGCGTCAGGAATTgg                                                       >  1:711158/1‑150 (MQ=255)
                                                                                        gCAACAGTTCGCCGCAACTACTCCAGCCCGCCGAATTTTGGTGCGCAGGTGGTGGCGGCAGTGCTGCATGACGAGGCATTGAAAGCCCGCTGGCTGGCGGAAGTAGAAGAGATGCGTACTCGCATTCTGGCAATGCGTCAGGAATTGGTg                                                     >  1:868697/1‑150 (MQ=255)
                                                                                          aaCAGTTCGCCGCAACTACTCCAGCCCGCCGAATTTTGGTGCGCAGGTGGTGGCGGCAGTGCTGAATGACGAGGCATTGAAAGCCAGCTGGCTGGCGGAAGTAGAAGAGATGCGTACTCGCATTCTGGCAATGCGTCAGGAATTGGTGAAg                                                  <  1:333366/151‑1 (MQ=255)
                                                                                           aCAGTTCGCCGCAACTACTCCAGCCCGCCGAATTTTGGTGCGCAGGTGGTGGCGGCAGTGCTGAATGACGAGGCATTGAAAGCCAGCTGGCTGGCGGAAGTAGAAGAGATGCGTACTCGCATTCTGGCAATGCGTCAGGAATTGGTGAAgg                                                 >  2:698010/1‑151 (MQ=255)
                                                                                              gTTCGCCGCAACTACTCCAGCCCGCCGAATTTTGGTGCGCAGGTGGTGGCGGCAGTGCTGAATGACGAGGCATTGAAAGCCAGCTGGCTGGCGGAAGTAGAAGAGATGCGTACTCGCATTCTGGCAATGCGTCAGGAATTGGTGAAGGTAt                                              >  1:866115/1‑151 (MQ=255)
                                                                                                  gccgcAACTACTCCAGCCCGCCGAATTTTGGTGCGCAGGTGGTGGCGGCAGTGCTGAATGACGAGGCATTGAAAGCCAGCTGGCTGGCGGAAGTAGAAGAGATGCGTACTCGCATTCTGGCAATGCGTCAGGAATTGGTGAAGGTATTAAg                                          <  2:931002/151‑1 (MQ=255)
                                                                                                   ccgcAGCTGCCCCTGCCCGTGTAATTTTGGTGTGCAGGTGGTGGCGGCAGTGCTGAATGACGAGGCATTGAAAACCAGCTGGCGTGCGGAAGTAGAAGAGATGCGTTCTCGCATTCTGTCAATGCGTCCGGAATTGGTGAAGGTAGTAAGc                                         <  1:520470/151‑1 (MQ=255)
                                                                                                         ctactCCAGCCCGCCGAATTTTGGTGCGCAGGTGGTGGCGGCAGTGCTGAATGACGAGGCATTGAAAGCCAGCTGGCTGGCGGAAGTAGAAGAGATGCGTACTCGCATTCTGGCAATGCGTCAGGAATTGGTGAAGGTATTAAGCACag                                     >  1:299927/1‑149 (MQ=255)
                                                                                                           actCCAGCCCGCCGAATTTTGGTGCGCAGGTGGTGGCGGCAGTGCTGAATGACGAGGCATTGAAAGCCAGCTGGCTGGCGGAAGTAGAAGAGATGCGTACTCGCATTCTGGCAATGCGTCAGGAATTGGTGAAGGTATTAAGCACAGAGAt                                 <  2:849871/151‑1 (MQ=255)
                                                                                                                 gCCCGCCGAATTTTGGTGCGCAGGTGGTGGCGGCAGTGCTGAATGACGAGGCATTGAAAGCCAGCTGGCTGGCGGAAGTAGAAGAGATGCGTACTCGCATTCTGGCAATGCGTCAGGAATTGGTGAAGGTATTAAGCACAGAGATGcc                              >  2:114385/1‑148 (MQ=255)
                                                                                                                  cccGCCGAATTTTGGTGCGCAGGTGGTGGCGGCAGTGCTGAATGACGTGGCATTTAAAGCCAGCTGGCTGGCGGAAGTAGAAGAGATGCGTACTCGAATTCTGGCAATGCGTCCGGACTTGGTGAAGGCATTAAGCACAGAGATCCCAGa                           >  1:706293/1‑150 (MQ=255)
                                                                                                                  cccGCCGAATTTTGGTGCGCAGGTGGTGGCGGCAGTGCTGAATGACGAGGCATTGAAAGCCAGCTGGCTGGCGGAAGTAGAAGAGATGCGTACTCGCATTCTGGCAATGCGTCAGGAATTGGTGAAGGTATTAAGCACAGAGATGCCAGa                           >  1:457408/1‑150 (MQ=255)
                                                                                                                    cgccgAATTTTGGTGCGCAGGTGGTGGCGGCAGTGCTGAATGACGAGGCATTGAAAGCCAGCTGGCTGGCGGAAGTAGAAGAGATGCGTACTCGCATTCTGGCAATGCGTCAGGAATTGGTGAAGGTATTAAGCACAGAGATGCCAGAACg                        <  1:824691/151‑1 (MQ=255)
                                                                                                                      ccgAATTTTGGTGCGCAGGTGGTGGCGGCAGTGCTGAATGACGAGGCATTGAAAGCCAGCTGGCTGGCGGAAGTAGAAGAGATGCGTACTCGCATTCTGGCAATGCGTCAGGAATTGGTGAAGGTATTAAGCACAGAGATGCCAGAACGCa                      <  1:467980/151‑1 (MQ=255)
                                                                                                                         aaTTTTGGTGCGCAGGTGGTGGCGGCAGTGCTGAATGACGAGGCATTGAAAGCCAGCTGGCTGGCGGAAGTAGAAGAGATGCGTACTCGCATTCTGGCAATGCGTCAGGAATTGGTGAAGGTATTAAGCACAGAGATGCCAGAACGCAAtt                   >  2:820901/1‑151 (MQ=255)
                                                                                                                            tttGGTGCGCAGGTGGTGGCGGCAGTGCTGAATGACGAGGCATTGAAAGCCAGCTGGCTGGCGGAAGTAGAAGAGATGCGTACTCGCATTCTGGCAATGCGTCAGGAATTGGTGAAGGTATTAAGCACAGAGATGCCAGAACGCAATTTc                 <  2:273393/150‑1 (MQ=255)
                                                                                                                            tttGGTGCGCAGGTGGTGGCGGCAGTGCTGAATGACGAGGCATTGAAAGCCAGCTGGCTGGCGGAAGTAGAAGAGATGCGTACTCGCATTCTGGCAATGCGTCAGGAATTGGTGAAGGTATTAAGCACAGAGATGCCAGAACGCAATTTCg                >  2:573743/1‑151 (MQ=255)
                                                                                                                               ggTGCGCAGGTGGTGGCGGCAGTGCTGAATGACGAGGCATTGAAAGCCAGCTGGCTGGCGGAAGTAGAAGAGATGCGTACTCGCATTCTGGCAATGCGTCAGGAATTGGTGAAGGTATTAAGCACAGAGATGCCAGAACGCAATTTCGAtt             <  1:191613/151‑1 (MQ=255)
                                                                                                                                  gcgcAGGTGGTGGCGGCAGTGCTGAATGACGAGGCATTGAAAGCCAGCTGGCTGGCGGAAGTAGAAGAGATGCGTACTCGCATTCTGGCAATGCGTCAGGAATTGGTGAAGGTATTAAGCACAGAGATGCCAGAACGCAATTTCGATTAt           <  2:389307/150‑1 (MQ=255)
                                                                                                                                    gcAGGTGGTGGCGGCAGTGCTGAATGACGAGGCATTGAAAGCCAGCTGGCTGGCGGAAGTAGAAGAGATGCGTACTCGCATTCTGGCAATGCGTCAGGAATTGGTGAAGGTATTAAGCACAGAGATGCCAGAACGCAATTTCGATTATCTg        <  2:677916/151‑1 (MQ=255)
                                                                                                                                    gcAGGTGGTGGCGGCAGTGCTGAATGACGAGGCATTGAAAGCCAGCTGGCTGGCGGAAGTAGAAGAGATGCGTACTCGCATTCTGGCAATGCGTCAGGAATTGGTGAAGGTATTAAGCACAGAGATGCCAGAACGCAATTTCGATTATCTg        <  2:917248/151‑1 (MQ=255)
                                                                                                                                      aGGTGGTGGCGGCAGTGCTGAATGACGAGGCATTGAAAGCCAGCTGGCTGGCGGAAGTAGAAGAGATGCGTACTCGCATTCTGGCAATGCGTCAGGAATTGGTGAAGGTATTAAGCACAGAGATGCCAGAACGCAATTTCGATTATCTGCt      >  2:426270/1‑151 (MQ=255)
                                                                                                                                       ggtggtGGCGGCAGTGCTGAATGACGAGGCATTGAAAGCCAGCTGGCTGGCGGAAGTAGAAGAGATGCGTACTCGCATTCTGGCAATGCGTCAGGAATTGGTGAAGGTATTAAGCACAGAGATGCCAGAACGCAATTTCGATTATCTGCtt     <  1:866004/151‑1 (MQ=255)
                                                                                                                                          ggtggCGGCAGTGCTGAATGACGAGGCATTGAAAGCCAGCTGGCTGGCGGAAGTAGAAGAGATGCGTACTCGCATTCTGGCAATGCGTCAGGAATTGGTGAAGGTATTAAGCACAGAGATGCCAGAACGCAATTTCGATTATCTGCTTAAt  <  1:365286/151‑1 (MQ=255)
                                                                                                                                          ggtggCGGCAGTGCTGAACGACGAGGCATTGAAAGCCAGCTGGCTGGCGGAAGTAGAAGAGATGCGTACTCGCATTCTGGCAATGCGTCAGGAATTGGTGAAGGTATTAAGCTCAGAGATGCCAGAACGCAATTTCGATTATCTGCTTaa   <  1:549825/150‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                
TTTCTCCCTTTACGGCGAGCGCGTCGGCGGACTTTCTGTTATGTGTGAAGATGCCGAAGCCGCTGGCCGCGTACTGGGGCAATTGAAAGCAACAGTTCGCCGCAACTACTCCAGCCCGCCGAATTTTGGTGCGCAGGTGGTGGCTGCAGTGCTGAATGACGAGGCATTGAAAGCCAGCTGGCTGGCGGAAGTAGAAGAGATGCGTACTCGCATTCTGGCAATGCGTCAGGAATTGGTGAAGGTATTAAGCACAGAGATGCCAGAACGCAATTTCGATTATCTGCTTAAT  >  NC_000913/4267857‑4268145

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑