Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,285,977	T→G	V465V (GTA→GTC)	acs ←	acetyl‑CoA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,285,977	0	T	G	100.0%	227.7 / NA	60	V465V (GTA→GTC)	acs	acetyl‑CoA synthetase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (37/23);  total (37/23)

GCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCG  >  NC_000913/4285829‑4286124                                                                                                                                                     |                                                                                                                                                    gCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATGAc                                                                                                                                                   <  1:413472/151‑1 (MQ=255)     ggTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATGACCAgg                                                                                                                                               >  2:735054/1‑151 (MQ=255)       ttATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATGACCAGGCt                                                                                                                                             >  2:435385/1‑151 (MQ=255)         aTCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATGACCAGGCTAc                                                                                                                                           >  2:359804/1‑151 (MQ=255)           ccAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATGACCAGGCTACCt                                                                                                                                         >  2:315595/1‑151 (MQ=255)                aaTAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATGACCAGGCTACCTTCgg                                                                                                                                     <  2:456313/150‑1 (MQ=255)                  tAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATGACCAGGCTACCTTCGGTgg                                                                                                                                  >  2:468104/1‑151 (MQ=255)                    gCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATGACCAGGCTACCTTCGGTGGcc                                                                                                                                >  1:189553/1‑151 (MQ=255)                     ccATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATGACCAGGCTACCTTCGGTGGccc                                                                                                                               <  1:722149/151‑1 (MQ=255)                           tCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATGACCAGGCTACCTTCGGTGGCCCCCTcc                                                                                                                          >  1:473691/1‑150 (MQ=255)                           tCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATGACCAGGCTACCTTCGGTGGCCCCCTc                                                                                                                           <  2:935442/149‑1 (MQ=255)                                gcgACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATGACCAGGCTACCTTCGGTGGCCCCCTCCAGCggg                                                                                                                    >  1:704750/1‑151 (MQ=255)                                 cgACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATGACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGt                                                                                                                   >  2:754751/1‑151 (MQ=255)                                 cgACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATGACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGt                                                                                                                   >  1:72492/1‑151 (MQ=255)                                   aCGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATGACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGtt                                                                                                                  >  1:98377/1‑150 (MQ=255)                                   aCGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATGACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGtt                                                                                                                  >  2:350012/1‑150 (MQ=255)                                   aCGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATGACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTa                                                                                                                 <  2:626112/151‑1 (MQ=255)                                      cgcgcCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATGACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCt                                                                                                              >  2:31169/1‑151 (MQ=255)                                        cgcCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATGACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTc                                                                                                            <  2:233429/151‑1 (MQ=255)                                         gcCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATGACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCg                                                                                                           >  2:389978/1‑151 (MQ=255)                                          ccGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATGACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGt                                                                                                          <  1:597678/151‑1 (MQ=255)                                           cGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATGACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGtt                                                                                                         <  1:730111/151‑1 (MQ=255)                                            gTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATGACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGtt                                                                                                         >  2:133787/1‑150 (MQ=255)                                               gccgcTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATGACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCg                                                                                                     <  1:201380/151‑1 (MQ=255)                                               gccgcTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATGACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCGTCgggagag                                                                                                     <  2:135334/151‑7 (MQ=255)                                                  gcTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATGACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGAcc                                                                                                  >  1:730049/1‑151 (MQ=255)                                                    tGAAATACATATTTTTGACGGTGGAGAAGTAGGTCTGTTCAAAACGATCGTGATCGCCAAACAGCGCACGCGCCTGACCCGGCCAGGAGCCGGTGATGACAAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGAcc                                                                                                  >  1:274206/1‑149 (MQ=255)                                                     gAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATGACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAgc                                                                                               >  2:647319/1‑151 (MQ=255)                                                        aTACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATGACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGcc                                                                                            <  2:443567/151‑1 (MQ=255)                                                          aCATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATGACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCgg                                                                                          >  1:432567/1‑151 (MQ=255)                                                               tttttGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATGACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGc                                                                                      >  2:545921/1‑150 (MQ=255)                                                                  ttGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATGACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGc                                                                                  >  1:191231/1‑151 (MQ=255)                                                                    gAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATGACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCg                                                                                <  1:321663/151‑1 (MQ=255)                                                                     aaGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATGACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCg                                                                                >  1:283512/1‑150 (MQ=255)                                                                      aGGGGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATGACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGcc                                                                                 >  1:527294/1‑148 (MQ=255)                                                                          ggAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGGCCCGGCCAGGAGTCGGTGATGACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCgaagaa                                                                           <  1:475265/150‑1 (MQ=255)                                                                            agaAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATGACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACgg                                                                        <  2:516119/151‑1 (MQ=255)                                                                                gTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATGACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACgt                                                                    >  2:521219/1‑151 (MQ=255)                                                                                  aGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATGACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACgtgt                                                                  >  2:295447/1‑151 (MQ=255)                                                                                  aGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATGACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACgtgt                                                                  <  2:793019/151‑1 (MQ=255)                                                                                    ggcTGTTCAAAACGTTCGTGATCGCCAAGCAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATGACCAGGCGGCCGGGGGTGGCGGCCGGCAGCGGGTTGCCTTCGTTATCGACCAGCGCCGGTGGCACGCCGAAGAACGGACGTGTTg                                                                <  2:196437/149‑1 (MQ=255)                                                                                    gTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATGACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTg                                                                >  1:134946/1‑151 (MQ=255)                                                                                    gTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATGACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTg                                                                >  2:190512/1‑151 (MQ=255)                                                                                       tGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATGACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCg                                                             >  2:909866/1‑151 (MQ=255)                                                                                                       gATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATGACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTc                                             >  2:695895/1‑151 (MQ=255)                                                                                                         tCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATGACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCgg                                           >  2:916111/1‑151 (MQ=255)                                                                                                             cAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATGACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAgc                                       <  1:636893/151‑1 (MQ=255)                                                                                                             cAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATGACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGCTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAg                                        >  1:147543/1‑150 (MQ=255)                                                                                                                       aCGCGCCTGACCCGGCCAGGAGTCGGTGATGACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGc                             <  1:730622/151‑1 (MQ=255)                                                                                                                        cgcgCCTGACCCGGCCAGGAGTCGGTGATGACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCg                            <  1:800321/151‑1 (MQ=255)                                                                                                                         gcgcCTGACCCGGCCAGGAGTCGGTGATGACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCgg                           >  1:896068/1‑151 (MQ=255)                                                                                                                         gcgcCTGACCCGGCCAGGAGTCGGTGATGACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCgg                           >  2:621309/1‑151 (MQ=255)                                                                                                                          cgcCTGACCCGGCCAGGAGTCGGTGATGACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCggg                          >  1:647927/1‑151 (MQ=255)                                                                                                                               gACCCGGCCAGGAGTCGGTGATGACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTg                       <  2:683187/149‑1 (MQ=255)                                                                                                                               gACCCGGCCAGGAGTCGGTGATGACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGAt                     >  2:731614/1‑151 (MQ=255)                                                                                                                               gACCCGGCCAGGAGTCGGTGATGACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGAt                     <  1:124687/151‑1 (MQ=255)                                                                                                                                aCCCGGCCAGGAGTCGGTGATGACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATc                    >  1:227312/1‑151 (MQ=255)                                                                                                                                          gAGTCGGTGATGACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAAccgc          >  1:334699/1‑151 (MQ=255)                                                                                                                                                 tgatgaCCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTc   >  1:813580/1‑151 (MQ=255)                                                                                                                                                  gATGACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTc   <  1:29970/150‑1 (MQ=255)                                                                                                                                                  gATGACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCg  <  1:468089/151‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                    GCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCG  >  NC_000913/4285829‑4286124

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑