Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,299,529	A→C	intergenic (‑163/+35)	fdhF ← / ← mdtP	formate dehydrogenase‑H, selenopolypeptide subunit/outer membrane factor of efflux pump

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,299,529	0	A	C	100.0%	211.5 / NA	57	intergenic (‑163/+35)	fdhF/mdtP	formate dehydrogenase‑H, selenopolypeptide subunit/outer membrane factor of efflux pump
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (27/30);  total (27/30)

TCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATGCCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATACCCGCCGCCCAGCGATTTCATCAACTGAATGCTTTGGATCACCCGGCGGCTGTCCAGCATCAGTAATGACATC  >  NC_000913/4299385‑4299669                                                                                                                                                 |                                                                                                                                             tcaaatcaCGCATACGCGCTCTCGACTACAGTATGCATCTTTTATGCCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGCAATAcc                                                                                                                                        >  1:762554/1‑151 (MQ=255)  caaatcaCGCATACGCGCTCTCGACTACAGTATGCATCTTTTATGCCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGCAATAcc                                                                                                                                        <  2:284384/150‑1 (MQ=255)  caaatcaCGCATACGCGCTCTCGACTACAGTATGCATCTTTTATGCCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGCAATACcg                                                                                                                                       >  2:865190/1‑151 (MQ=255)          gCATACGCGCTCTCGACTACAGTATGCATCTTTTATGCCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGCAATACCGCGTGACAg                                                                                                                               <  2:285355/151‑1 (MQ=255)              aCGCGCTCTCGACTACAGTATGCATCTTTTATGCCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGCAATACCGCGTGACAGCCAt                                                                                                                           <  1:637785/151‑1 (MQ=255)                 cgcTCTCGACTACAGTATGCATCTTTTATGCCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGCAATACCGCGTGACAGCCATCAc                                                                                                                        <  2:235118/151‑1 (MQ=255)                         aCTACAGTATGCATCTTTTATGCCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGCAATACCGCGTGACAGCCATCACGCGGc                                                                                                                   >  2:499788/1‑148 (MQ=255)                            aCAGTATGCATCTTTTATGCCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGCAATACCGCGTGACAGCCATCACGCGGCAGACa                                                                                                              <  2:138053/150‑1 (MQ=255)                                tATGCATCTTTTATGCCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGCAATACCGCGTGACAGCCATCACGCGGCAGACatttt                                                                                                          >  2:714141/1‑150 (MQ=255)                                   gCATCTTTTATGCCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGCAATACCGCGTGACAGCCATCACGCGGCAGACattttattt                                                                                                      <  2:871158/151‑1 (MQ=255)                                     aTCTTTTATGCCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGCAATACCGCGTGACAGCCATCACGCGGCAGACATTTTAttttt                                                                                                    >  1:354047/1‑151 (MQ=255)                                         tttATGCCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGCAATACCGCGTGACAGCCATCACGCGGCAGACATTTTAttttt                                                                                                    <  2:560796/147‑1 (MQ=255)                                          ttATGCCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGCAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTcg                                                                                               >  2:620190/1‑151 (MQ=255)                                           tATGCCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGCAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTcg                                                                                               >  1:577780/1‑150 (MQ=255)                                            aTGCCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGCAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTcgac                                                                                             >  1:62107/1‑151 (MQ=255)                                             tGCCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGCAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTcgacg                                                                                            >  2:217359/1‑151 (MQ=255)                                               ccACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGCAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCgacgac                                                                                          <  1:360946/151‑1 (MQ=255)                                               ccACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGCAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCgacgac                                                                                          >  2:717787/1‑151 (MQ=255)                                                 acaTTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGCAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACgg                                                                                        <  1:799605/151‑1 (MQ=255)                                                 acaTTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGCAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACgg                                                                                        <  2:420574/151‑1 (MQ=255)                                                  cagtttAGGTGGGGTCATTCCCTTATATTACGGGCACTATTTATTCCAAACCCTGTCGAACATCAGGCTGACGCCAGTTTTGACGTGACGAAACGCAACACCGCGGGACAGCCATCACGCGGCAGTCACTTTATTTATACTcgac                                                                                             <  1:597048/142‑1 (MQ=255)                                                     tttATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGCAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGAcc                                                                                    >  2:261606/1‑151 (MQ=255)                                                       tATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGCAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGAcctg                                                                                  >  1:250408/1‑151 (MQ=255)                                                       tATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGCAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGAcct                                                                                   <  1:642099/150‑1 (MQ=255)                                                         tgtgGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGCAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGAcctgcc                                                                                >  2:695728/1‑151 (MQ=255)                                                                 cATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGCAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATAccc                                                                        >  2:207318/1‑151 (MQ=255)                                                                  aTTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGCAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATACccg                                                                       >  2:379540/1‑151 (MQ=255)                                                                     cccTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGCAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATACccgcc                                                                     >  1:923005/1‑150 (MQ=255)                                                                         gATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGCAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATACCCGCCGccc                                                                 >  1:69692/1‑150 (MQ=255)                                                                         gATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGCAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATACCCGCCGccc                                                                 <  2:312229/150‑1 (MQ=255)                                                                         gATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGCAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATACCCGCCGCCCa                                                                <  1:451710/151‑1 (MQ=255)                                                                          atatTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGCAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATACCCGCCGccc                                                                 >  2:646833/1‑149 (MQ=255)                                                                          atatTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGCAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATACCCGCCGCCCAg                                                               <  1:656315/151‑1 (MQ=255)                                                                              tACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGCAATAACGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATACCCGCCGCCCAGCGAt                                                           >  1:448365/1‑151 (MQ=255)                                                                                        atttattCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGCAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATACTCGCCGCCCAGCGATTTCATCaa                                                   <  2:315994/149‑1 (MQ=255)                                                                                          ttattCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGCAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATACCCGCCGCCCAGCGATTTCATCAACTg                                                >  2:208003/1‑150 (MQ=255)                                                                                          ttattCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGCAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATACCCGCCGCCCAGCGATTTCATCAACTg                                                <  1:314555/150‑1 (MQ=255)                                                                                          ttattCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGCAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATACCCGCCGCCCAGCGATTTCATCAACTg                                                <  1:577767/150‑1 (MQ=255)                                                                                           tattCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGCAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATACCCGCCGCCCAGCGATTTCATCAACTGa                                               >  1:748393/1‑150 (MQ=255)                                                                                              tCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGCAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATACCCGCCGCCCAGCGATTTCATCAACTGAATGc                                           >  1:679747/1‑151 (MQ=255)                                                                                                    ctctGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGCAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATACCCGCCGCCCAGCGATTTCATCAACTGAATGCTTTgg                                      >  2:443174/1‑150 (MQ=255)                                                                                                     tctGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGCAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATACCCGCCGCCCAGCGATTTCATCAACTGAATGCTTTGGAt                                    >  1:673175/1‑151 (MQ=255)                                                                                                       tGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGCAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATACCCGCCGCCCAGCGATTTCATCAACTGAATGCTTTGGATc                                   >  1:639887/1‑150 (MQ=255)                                                                                                        gACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGCAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATACCCGCCGCCCAGCGATTTCATCAACTGAATGCTTTGGATCa                                  <  2:762573/150‑1 (MQ=255)                                                                                                        gACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGCAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATACCCGCCGCCCAGCGATTTCATCAACTGAATGCTTTGGATCAc                                 <  2:192013/151‑1 (MQ=255)                                                                                                          cGAAAAGCAGGCTGTCGTCAGTTTTGACGTGACGAATCGCAATACCGCGTGGCAGCCATCACGCGGCAGACATTTTATTTTTGCGCGACGACGGGACCTGCCTGATACCCGCCGCCCAGCGATGTCATCAACTGAATGCTTTGGATCAc                                 <  1:596697/149‑1 (MQ=255)                                                                                                          cGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGCAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATACCCGCCGCCCAGCGATTTCATCAACTGAATGCTTTGGATCAccc                               <  1:110640/151‑1 (MQ=255)                                                                                                             aaaaCAGGCTGTCGTCAGTTTTGACGTGACGAAACGCAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATACCCGCCGCCCAGCGATTTCATCAACTGAATGCTTTGGATCACCcggc                            <  1:886708/151‑1 (MQ=255)                                                                                                             aaaaCAGGCTGTCGTCAGTTTTGACGTGACGAAACGCAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATACCCGCCGCCCAGCGATTTCATCAACTGAATGCTTTGGATCACCcggc                            <  2:821754/151‑1 (MQ=255)                                                                                                              aaaCAGGCTGTCGTCAGTTTTGACGTGACGAAACGCAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATACCCGCCGCCCAGCGATTTCATCAACTGAATGCTTTGGATCACCcggcg                           >  1:93855/1‑151 (MQ=255)                                                                                                                 cAGGCTGTCGTCAGTTTTGACGTGACGAAACGCAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATACCCGCCGCCCAGCGATTTCATCAACTGAATGCTTTGGATCACCCGGCGGCt                        <  2:881455/151‑1 (MQ=255)                                                                                                                  aGGCTGTCGTCAGTTTTGACGTGACGAAACGCAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATACCCGCCGCCCAGCGATTTCATCAACTGAATGCTTTGGATCACCCGGCGGCTg                       <  2:149071/151‑1 (MQ=255)                                                                                                                            cAGTTTTGACGTGACGAAACGCAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATACCCGCCGCCCAGCGATTTCATCAACTGAATGCTTTGGATCACCCGGCGGCTGTCCAGCATCa             <  1:187445/151‑1 (MQ=255)                                                                                                                                tttGACGTGACGAAACGCAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATACCCGCCGCCCAGCGATTTCATCAACTGAATGCTTTGGATCACCCGGCGGCTGTCCAGCATCAGTa          <  2:417921/150‑1 (MQ=255)                                                                                                                                  tgacgtgacgAAACGCAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATACCCGCCGCCCAGCGATTTCATCAACTGAATGCTTTGGATCACCCGGCGGCTGTCCAGCATCAGTAAt        <  2:210801/150‑1 (MQ=255)                                                                                                                                        gacgAAACGCAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATACCCGCCGCCCAGCGATTTCATCAACTGAATGCTTTGGATCACCCGGCGGCTGTCCAGCATCAGTAATGACAtc  >  2:71172/1‑150 (MQ=255)                                                                                                                                        gacgAAACGCAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATACCCGCCGCCCAGCGATTTCATCAACTGAATGCTTTGGATCACCCGGCGGCTGTCCAGCATCAGTAATGACAtc  <  2:175325/150‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                             TCAAATCACGCATACGCGCTCTCGACTACAGTATGCATCTTTTATGCCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATACCCGCCGCCCAGCGATTTCATCAACTGAATGCTTTGGATCACCCGGCGGCTGTCCAGCATCAGTAATGACATC  >  NC_000913/4299385‑4299669

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑