Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,310,903	T→G	L26F (TTA→TTC)	alsA ←	D‑allose ABC transporter ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,310,903	0	T	G	100.0%	184.5 / NA	49	L26F (TTA→TTC)	alsA	D‑allose ABC transporter ATPase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (21/28);  total (21/28)

TTATGATCCAGCTTGTTATAGCTAATGTTATTAATGGTAATGGTGCCTTTGGTCGGCTCATGTATTCCGGATAAAACTTTCATTAGCGTGGATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCA  >  NC_000913/4310757‑4311046                                                                                                                                                   |                                                                                                                                                ttATGATCCAGCTTGTTATAGCTAATGTTATTAATGGTAATGGTGCCTTTGGTCGGCTCATGTATTCCGGATAAAACTTTCATTAGCGTGGATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTGAAAt                                                                                                                                             >  1:519669/1‑151 (MQ=255)   aTGATCCAGCTTGTTATAGCTAATGTTATTAATGGTAATGGTGCCTTTGGTCGGCTCATGTATTCCGGATAAAACTTTCATTAGCGTGGATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTGAAAtt                                                                                                                                            >  1:844665/1‑150 (MQ=255)   aTGATCCAGCTTGTTATAGCTAATGTTATTAATGGTAATGGTGCCGTTGGTCGGCTCATGTATTCCGGATAAAACTTTCATTAGCGTGGATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTGAAATTa                                                                                                                                           <  2:103256/151‑1 (MQ=255)   aTGATCCAGCTTGTTATAGCTAATGTTATAAATGGTAATGGTGCCTTTGGTCGGCTCATGTATTCCGGATAAAACTTTCATTAGCGTGGATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTGAAATTa                                                                                                                                           <  1:401815/151‑1 (MQ=255)     gATCCAGCTTGTTATAGCTAATGTTATTAATGGTAATGGTGCCTTTGGTCGGCTCATGTATTCCGGATAAAACTTTCATTAGCGTGGATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTGAAATTAAc                                                                                                                                         <  2:80038/151‑1 (MQ=255)            cTTGTTATAGCTAATGTTATTAATGGTAATGGTGCCTTTGGTCGGCTCATGTATTCCGGATAAAACTTTCATTAGCGTGGATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTGAAATTAACCGACttt                                                                                                                                  >  1:874704/1‑151 (MQ=255)                  ataGCTAATGTTATTAATGGTAATGGTGCCTTTGGTCGGCTCATGTATTCCGGATAAAACTTTCATTAGCGTGGATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTGAAATTAACCGACTTTAATgag                                                                                                                            <  2:369284/151‑3 (MQ=255)                    aGCTAATGTTATTAATGGTAATGGTGCCTTTGGTCGGCTCATGTATTCCGGATAAAACTTTCATTAGCGTGGATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTGAAATTAACCGACTTTAATGCGt                                                                                                                           <  2:911440/150‑1 (MQ=255)                           gTTATTAATGGTAATGGTGCCTTTGGTCGGCTCATGTATTCCGGATAAAACTTTCATTAGCGTGGATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTGAAATTAACCGACTTTAATGCGTGAACCGGa                                                                                                                   <  1:287038/151‑1 (MQ=255)                           gTTATTAATGGTAATGGTGCCTTTGGTCGGCTCATGTATTCCGGATAAAACTTTCATTAGCGTGGATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTAGTTCACCAGGATAAGCCGTGAAATTAACCGACTTTAATGCGTGAACCgg                                                                                                                    <  1:214219/150‑1 (MQ=255)                              attaATGGTAATGGTGCCTTTGGTCGGCTCATGTATTCCGGATAAAACTTTCATTAGCGTGGATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTGAAATTAACCGACTTTAATGCGTGAACCGGAcc                                                                                                                 <  1:54098/150‑1 (MQ=255)                              attaATGGTAATGGTGCCTTTGGTCGGCTCATGTATTCCGGATAAAACTTTCATTAGCGTGGATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTGAAATTAACCGACTTTAATGCGTGAACCGGACCa                                                                                                                <  1:146862/151‑1 (MQ=255)                               ttaATGGTAATGGTGCCTTTGGTCGGCTCATGTATTCCGGATAAAACTTTCATTAGCGTGGATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTGAAATTAACCGACTTTAATGCGTGAACCGGACCa                                                                                                                >  1:657222/1‑150 (MQ=255)                                taatggtaatggTGCCTTTGGTCGGCTCATGTATTCCGGATAAAACTTTCATTAGCGTGGATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTGAAATTAACCGACTTTAATGCGTGAACCGGACCaa                                                                                                               <  1:801505/150‑1 (MQ=255)                                taatggtaatggTGCCTTTGGTCGGCTCATGTATTCCGGATAAAACTTTCATTAGCGTGGATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTGAAATTAACCGACTTTAATGCGTGAACCGGACCaa                                                                                                               >  2:742303/1‑150 (MQ=255)                                       aatggtGCCTTTGGTCGGCTCATGTATTCCGGATAAAACTTTCATTAGCGTGGATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTGAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTg                                                                                                       <  1:855763/151‑1 (MQ=255)                                          ggtGCCTTTGGTCGGCTCATGTATTCCGGATAAAACTTTCATTAGCGTGGATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTGAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCg                                                                                                    <  1:830214/151‑1 (MQ=255)                                             gCCTTTGGTCGGCTCATGTATTCCGGATAAAACTTTCATTAGCGTGGATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTGAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATc                                                                                                 >  2:546120/1‑151 (MQ=255)                                              ccTTTGGTCGGCTCATGTATTCCGGATAAAACTTTCATTAGCGTGGATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTGAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATcc                                                                                                >  2:501369/1‑151 (MQ=255)                                               cTTTGGTCGGCTCATGTATTCCGGATAAAACTTTCATTAGCGTGGATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTGAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATccc                                                                                               >  1:246132/1‑151 (MQ=255)                                                        gCTCATGTATTCCGGATAAAACTTTCATTAGCGTGGATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTGAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGa                                                                                      <  1:601926/151‑1 (MQ=255)                                                           cATGTATTCCGGATAAAACTTTCATTAGCGTGGATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTGAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGat                                                                                     <  2:867120/149‑1 (MQ=255)                                                              gTATTCCGGATAAAACTTTCATTAGCGTGGATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTGAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGatatat                                                                                 <  2:202113/150‑1 (MQ=255)                                                                aTTCCGGATAAAACTTTCATTAGCGTGGATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTGAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATg                                                                              >  2:85746/1‑151 (MQ=255)                                                                 ttCCGGATAAAACTTTCATTAGCGTGGATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTGAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATgg                                                                             >  2:272485/1‑151 (MQ=255)                                                                 ttCCGGATAAAACTTTCATTAGCGTGGATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTGAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATg                                                                              <  1:598216/150‑1 (MQ=255)                                                                  tCCGGATAAAACTTTCATTAGCGTGGATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTGAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATgg                                                                             <  1:104172/150‑1 (MQ=255)                                                                  tCCGGATAAAACTTTCATTAGCGTGGATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTGAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGc                                                                            >  1:517493/1‑151 (MQ=255)                                                                     ggATAAAACTTTCATTAGCGTGGATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTGAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTg                                                                         >  1:284241/1‑151 (MQ=255)                                                                         aaaaCTTTCATTAGCGTGGATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTGAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGcc                                                                      <  1:54782/150‑1 (MQ=255)                                                                                  aTTAGCGTGGATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTGAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGCCATAAATAAcc                                                            <  1:529393/151‑1 (MQ=255)                                                                                      gCGTGGATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTGAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGCCATAAATAACctctc                                                        <  1:235022/151‑1 (MQ=255)                                                                                       cGTGGATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTGAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGCCATAAATAACctctct                                                       >  2:528891/1‑151 (MQ=255)                                                                                                 cccGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTGAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAat                                              >  2:927517/1‑150 (MQ=255)                                                                                                    gcgcCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTGAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAATATCg                                          <  1:399312/151‑1 (MQ=255)                                                                                                    gcgcCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTGAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAATATCg                                          >  1:229227/1‑151 (MQ=255)                                                                                                         aTTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTGAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAATATCGtatta                                     <  2:224396/151‑1 (MQ=255)                                                                                                         aTTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTGAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAATATCGtatta                                     <  2:166391/151‑1 (MQ=255)                                                                                                                   aGTAATGCATGTATTTCACCAGGATAAACCGTGAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGAt                            <  1:734457/150‑1 (MQ=255)                                                                                                                     tAATGCATGTATTTCACCAGGATAAACCGTGAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGc                         >  2:503041/1‑151 (MQ=255)                                                                                                                       aTGCATGTATTTCACCAGGATAAACCGTGAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCt                        >  2:620505/1‑150 (MQ=255)                                                                                                                           aTGTATTTCACCAGGATAAACCGTGAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACAtt                   >  1:438105/1‑151 (MQ=255)                                                                                                                               aTTTCACCAGGATAAACCGTGAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATc               <  2:754919/151‑1 (MQ=255)                                                                                                                                  tCACCAGGATAAACCGTGAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAgg            >  2:413318/1‑151 (MQ=255)                                                                                                                                  tCACCAGGATAAACCGTGAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAgg            >  1:57382/1‑151 (MQ=255)                                                                                                                                   cACCAGGATAAACCGTGAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGt           <  2:17606/151‑1 (MQ=255)                                                                                                                                      cAGGATAAACCGTGAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCt         >  1:552027/1‑150 (MQ=255)                                                                                                                                            aaaCCGTGAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCa  <  1:496493/151‑1 (MQ=255)                                                                                                                                            aaaCCGTGAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCa  <  1:546102/151‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                TTATGATCCAGCTTGTTATAGCTAATGTTATTAATGGTAATGGTGCCTTTGGTCGGCTCATGTATTCCGGATAAAACTTTCATTAGCGTGGATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCA  >  NC_000913/4310757‑4311046

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑