Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,351,371	T→G	I98L (ATT→CTT)	dcuS ←	sensory histidine kinase in two‑component regulatory system with DcuR, regulator of anaerobic fumarate respiration

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,351,371	0	T	G	100.0%	217.7 / NA	58	I98L (ATT→CTT)	dcuS	sensory histidine kinase in two‑component regulatory system with DcuR, regulator of anaerobic fumarate respiration
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (36/22);  total (36/22)

AAAGCCTGCGCCAGAAAACCGCGATTGATAGCGACATTTTCTTCGCCATTCAGCGCTTTAAGGATGTCATCACCTTTAAATGGCTGACCAATACGCTGGGCTTCAGGATGCGAGTAGCGAAGACTTTGCATATCGGTAACGACAATAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGCCACTCTCCTGCGGTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCT  >  NC_000913/4351226‑4351516                                                                                                                                                  |                                                                                                                                                  aaaGCCTGCGCCAGAAAACCGCGATTGATAGCGACATTTTCTTCGCCATTCAGCGCTTTAAGGATGTCATCACCTTTAAATGGCTGACCAATACGCTGGGCTTCAGGATGCGAGTAGCGAAGACTTTGCATATCGGTAACGACAAGAAAca                                                                                                                                              <  2:707511/151‑1 (MQ=255)    gCCTGCGCCAGAAAACCGCGATTGATAGCGACATTTTCTTCGCCATTCAGCGCTTTAAGGATGTCATCACCTTTAAATGGCTGACCAATACGCTGGGCTTCAGGATGCGAGTAGCGAAGACTTTGCATATCGGTAACGACAAGAAAcagca                                                                                                                                           <  2:403772/151‑1 (MQ=255)       tGCGCCAGAAAACCGCGATTGATAGCGACATTTTCTTCGCCATTCAGCGCTTTAAGGATGTCATCACCTTTAAATGGCTGACCAATACGCTGGGCTTCAGGATGCGAGTAGCGAAGACTTTGCATATCGGTAACGACAAGAAAcagc                                                                                                                                            >  1:523313/1‑147 (MQ=255)         cgcCAGAAAACCGCGATTGATAGCGACATTTTCTTCGCCATTCAGCGCTTTAAGGATGTCATCACCTTTAAATGGCTGACCAATACGCTGGGCTTCAGGATGCGAGTAGCGAAGACTTTGCATATCGGTAACGACAAGAAACAGCAGATc                                                                                                                                       <  1:701864/150‑1 (MQ=255)            cAGAAAACCGCGATTGATAGCGACATTTTCTTCGCCATTCAGCGCTTTAAGGATGTCATCACCTTTAAATGGCTGACCAATACGCTGGGCTTCAGGATGCGAGTAGCGAAGACTTTGCATATCGGTAACGACAAGAAACAGCAGATCGttg                                                                                                                                   <  2:365694/151‑1 (MQ=255)             aGAAAACCGCGATTGATAGCGACATTTTCTTCGCCATTCAGCGCTTTAAGGATGTCATCACCTTTAAATGGCTGACCAATACGCTGGGCTTCAGGATGCGAGTAGCGAAGACTTTGCATATCGGTAACGACAAGAAACAGCAGATCGttgc                                                                                                                                  >  2:930998/1‑151 (MQ=255)                 aaCCGCGATTGATAGCGACATTTTCTTCGCCATTCAGCGCTTTAAGGATGTCATCACCTTTAAATGGCTGACCAATACGCTGGGCTTCAGGATGCGAGTAGCGAAGACTTTGCATATCGGTAACGACAAGAAACAGCAGATCGttgcgttt                                                                                                                              <  1:603534/151‑1 (MQ=255)                      cgATTGATAGCGACATTTTCTTCGCCATTCAGCGCTTTTAGGATGTCATCACCTTTAAATGGCTGACCAATACGCTGGGCTTCAGGATGCGAGTAGCGAAGACTTTGCATATCGGTAACGACAAGAAACAGCAGATCGttgcgtttgcgt                                                                                                                          >  2:789397/1‑150 (MQ=255)                       gATTGATAGCGACATTTTCTTCGCCATTCAGCGCTTTAAGGATGTCATCACCTTTAAATGGCTGACCAATACGCTGGGCTTCAGGATGCGAGTAGCGAAGACTTTGCATATCGGTAACGACAAGAAACAGCAGATCGTTGCGTTTGCGTAc                                                                                                                        >  2:617648/1‑151 (MQ=255)                        aTTGATAGCGACATTTTCTTCGCCATTCAGCGCTTTAAGGATGTCATCACCTTTAAATGGCTGACCAATACGCTGGGCTTCAGGATGCGAGTAGCGAAGACTTTGCATATCGGTAACGACAAGAAACAGCAGATCGTTGCGTTTGCGTACg                                                                                                                       <  1:263089/151‑1 (MQ=255)                        aTTGATAGCGACATTTTCTTCGCCATTCAGCGCTTTAAGGATGTCATCACCTTTAAATGGCTGACCAATACGCTGGGCTTCAGGATGCGAGTAGCGAAGACTTTGCATATCGGTAACGACAAGAAACAGCAGATCGTTGCGTTTGCGTACg                                                                                                                       >  1:527867/1‑151 (MQ=255)                               gCGACATTTTCTTCGCCATTCAGCGCTTTAAGGATGTCATCACCTTTAAATGGCTGACCAATACGCTGGGCTTCAGGATGCGAGTAGCGAAGACTTTGCATATCGGTAACGACAAGAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCcg                                                                                                                >  1:583942/1‑151 (MQ=255)                                  aCATTTTCTTCGCCATTCAGCGCTTTAAGGATGTCATCACCTTTAAATGGCTGACCAATACGCTGGGCTTCAGGATGCGAGTAGCGAAGACTTTGCATATCGGTAACGACAAGAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCcgcg                                                                                                              >  2:292793/1‑150 (MQ=255)                                   cATTTTCTTCGCCATTCAGCGCTTTAAGGATGTCATCACCTTTAAATGGCTGACCAATACGCTGGGCTTCAGGATGCGAGTAGCGAAGACTTTGCATATCGGTAACGACAAGAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGAt                                                                                                            <  2:588455/151‑1 (MQ=255)                                   cATTTTCTTCGCCATTCAGCGCTTTAAGGATGTCATCACCTTTAAATGGCTGACCAATACGCTGGGCTTCAGGATGCGAGTAGCGAAGACTTTGCATATCGGTAACGACAAGAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGAt                                                                                                            >  1:65818/1‑151 (MQ=255)                                         cttcGCCATTCAGCGCTTTAAGGATGTCATCACCTTTAAATGGCTGACCAATACGCTGGGCTTCAGGATGCGAGTAGCGAAGACTTTGCATATCGGTAACGACAAGAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTg                                                                                                      >  1:272881/1‑151 (MQ=255)                                         cttcGCCATTCAGCGCTTTAAGGATGTCATCACCTTTAAAAGGCTGACCAATACGCTGGGCTTCAGGATGCGAGTAGCGAAGACTTTGCATATCGGTAACGACAAGAAACAGCAGATCGTTGCGATTGCGTACGGCTTCCGCGATGGCCTg                                                                                                      >  2:448627/1‑151 (MQ=255)                                           tcGCCATTCAGCGCTTTAAGGATGTCATCACCTTTAAATGGCTGACCAATACGCTGGGCTTCAGGATGCGAGTAGCGAAGACTTTGCATATCGGTAACGACAAGAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTgg                                                                                                     <  1:73335/150‑1 (MQ=255)                                            cGCCATTCAGCGCTTTAAGGATGTCATCACCTTTAAATGGCTGACCAATACGCTGGGCTTCAGGATGCGAGTAGCGAAGACTTTGCATATCGGTAACGACAAGAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGAt                                                                                                   <  1:542262/151‑1 (MQ=255)                                                aTTCAGCGCTTTAAGGATGTCATCACCTTTAAATGGCTGACCAATACGCTGGGCTTCAGGATGCGAGTAGCGAAGACTTTGCATATCGGTAACGACAAGAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGcc                                                                                                >  1:737206/1‑150 (MQ=255)                                                   cAGCGCTTTAAGGATGTCATCACCTTTAAATGGCTGACCAATACGCTGGGCTTCAGGATGCGAGTAGCGAAGACTTTGCATATCGGTAACGACAAGAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGCCActc                                                                                            >  2:150299/1‑151 (MQ=255)                                                      cgcTTTAAGGATGTCATCACCTTTAAATGGCTGACCAATACGCTGGGCTTCAGGATGCGAGTAGCGAAGACTTTGCATATCGGTAACGACAAGAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGCCACTCTcc                                                                                         >  2:210518/1‑151 (MQ=255)                                                          ttAAGGATGTCATCACCTTTAAATGGCTGACCAATACGCTGGGCTTCAGGATGCGAGTAGCGAAGACTTTGCATATCGGTAACGACAAGAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGCCACTCTCCTGCg                                                                                     >  2:730341/1‑151 (MQ=255)                                                            aaGGATGTCATCACCTTTAAATGGCTGACCAATACGCTGGGCTTCAGGATGCGAGTAGCGAAGACTTTGCATATCGGTAACGACAAGAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGCCACTCTCCTGCgg                                                                                    >  1:30297/1‑150 (MQ=255)                                                            aaGGATGTCATCACCTTTAAATGGCTGACCAATACGCTGGGCTTCAGGATGCGAGTAGCGAAGACTTTGCATATCGGTAACGACAAGAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGCCACTCTCCTGCGGt                                                                                   <  1:692765/151‑1 (MQ=255)                                                              ggATGTCATCACCTTTAAATGGCTGACCAATACGCTGGGCTTCAGGATGCGAGTAGCGAAGACTTTGCATATCGGTAACGACAAGAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGCCACTCTCCTGCGGttt                                                                                 >  1:126799/1‑151 (MQ=255)                                                               gATGTCATCACCTTTAAATGGCTGACCAATACGCTGGGCTTCAGGATGCGAGTAGCGAAGACTTTGCATATCGGTAACGACAAGAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGCCACTCTCCTGCGGtttt                                                                                <  2:774532/151‑1 (MQ=255)                                                                   tcatcaCCTTTAAATGGCTGACCAATACGCTGGGCTTCAGGATGCGAGTAGCGAAGACTTTGCATATCGGTAACGACAAGAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGCCACTCTCCTGCGGTTTTTTCt                                                                            >  2:88452/1‑151 (MQ=255)                                                                    catcaCCTTTAAATGGCTGACCAATACGCTGGGCTTCAGGATGCGAGTAGCGAAGACTTTGCATATCGGTAACGACAAGAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGCCACTCTCCTGCGGTTTTTTCTg                                                                           >  2:494115/1‑151 (MQ=255)                                                                       caCCTTTAAATGGCTGACCAATACGCTGGGCTTCAGGATGCGAGTAGCGAAGACTTTGCATATCGGTAACGACAAGAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGCCACTCTCCTGCGGTTTTTTCTGCa                                                                         >  1:259327/1‑150 (MQ=255)                                                                       caCCTTTAAATGGCTGACCAATACGCTGGGCTTCAGGATGCGAGTAGCGAAGACTTTGCATATCGGTAACGACAAGAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGCCACTCTCCTGCGGTTTTTTCTGCa                                                                         >  2:159047/1‑150 (MQ=255)                                                                        aCCTTTAAATGGCTGACCAATACGCTGGGCTTCAGGATGCGAGTAGCGAAGACTTTGCATATCGGTAACGACAAGAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGCCACTCTCCTGCGGTTTTTTCTGCa                                                                         >  2:6788/1‑149 (MQ=255)                                                                             tAAATGGCTGACCAATACGCTGGGCTTCAGGATGCGAGTAGCGAAGACTTTGCATATCGGTAACGACAAGAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGCCACTCTCCTGCGGTTTTTTCTGCAAGCCCt                                                                   >  1:885196/1‑150 (MQ=255)                                                                                     tGACCAATACGCTGGGCTTCAGGATGCGAGTAGCGAAGACTTTGCATATCGGTAACGACAAGAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGCCACTCTCCTGCGGTTTTTTCTGCAAGCCCTGACGGAttt                                                          >  2:476897/1‑151 (MQ=255)                                                                                      gACCAATACGCTGGGCTTCAGGATGCGAGTAGCGAAGACTTTGCATATCGGTAACGACAAGAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGCCACTCTCCTGCGGTTTTTTCTGCAAGCCCTGACGGAtt                                                           <  2:634938/149‑1 (MQ=255)                                                                                      gACCAATACGCTGGGCTTCAGGATGCGAGTAGCGAAGACTTTGCATATCGGTAACGACAAGAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGCCACTCTCCTGCGGTTTTTTCTGCAAGCCCTGACGGAtt                                                           <  1:917125/149‑1 (MQ=255)                                                                                       aCCAATACGCTGGGCTTCAGGATGCGAGTAGCGAAGACTTTGCATATCGGTAACGACAAGAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGCCACTCTCCTGCGGTTTTTTCTGCAAGCCCTGACGGATTTcc                                                        >  2:618182/1‑151 (MQ=255)                                                                                        ccAATACGCTGGGCTTCAGGATGCGAGTAGCGAAGACTTTGCATATCGGTAACGACAAGAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGCCACTCTCCTGCGGTTTTTTCTGCAAGCCCTGACGGATTTCCg                                                       <  2:408762/151‑1 (MQ=255)                                                                                        ccAATACGCTGGGCTTCAGGATGCGAGTAGCGAAGACTTTGCATATCGGTAACGACAAGAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGCCACTCTCCTGCGGTTTTTTCTGCAAGCCCTGACGGATTTCCg                                                       <  1:5543/151‑1 (MQ=255)                                                                                              cGCTGGGCTTCAGGATGCGAGTAGCGAAGACTTTGCATATCGGTAACGACAAGAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGCCACTCTCCTGCGGTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGt                                                 >  2:586445/1‑151 (MQ=255)                                                                                                cTGGGCTTCAGGATGCGAGTAGCGAAGACTTTGCATATCGGTAACGACAAGAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGCCACTCTCCTGCGGTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCg                                               >  1:510109/1‑151 (MQ=255)                                                                                                  gggCTTCAGGATGCGAGTAGCGAAGACTTTGCATATCGGTAACGACAAGAAACAGCAGATCGTTGCGTTTGCGTTCGGCTTCCGCGATGGCCTGGATGCCACTCTCCTGCGGTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGc                                             <  2:132305/151‑1 (MQ=255)                                                                                                  gggCTTCAGGATGCGAGTAGCGAAGACTTTGCATATCGGTAACGACAAGAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGCCACTCTCCTGCGGTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCgg                                              <  1:934503/150‑1 (MQ=255)                                                                                                       tCAGGATGCGAGTAGCGAAGACTTTGCATATCGGTAACGACAAGAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGCCACTCTCCTGCGGTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAg                                          <  2:141120/149‑1 (MQ=255)                                                                                                           gATGCGAGTAGCGAAGACTTTGCATATCGGTAACGACAAGAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGCCACTCTCCTGCGGTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTAcg                                    >  1:119361/1‑151 (MQ=255)                                                                                                                   tAGCGAAGACTTTGCATATCGGTAACGACAAGAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGCCACTCTCCTGCGGTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTg                            >  1:882096/1‑151 (MQ=255)                                                                                                                   tAGCGAAGACTTTGCATATCGGTAACGACAAGAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGCCACTCTCCTGCGGTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTg                            >  2:747992/1‑151 (MQ=255)                                                                                                                    aGCGAAGACTTTGCATATCGGTAACGACAAGAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGCCACTCTCCTGCGGTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGc                           <  2:606451/151‑1 (MQ=255)                                                                                                                         aGACTTTGCATATCGGTAACGACAAGAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGCCACTCTCCTGCGGTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTg                      >  1:475573/1‑151 (MQ=255)                                                                                                                         aGACTTTGCATATCGGTAACGACAAGAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGCCACTCTCCTGCGGTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTg                      <  1:245980/151‑1 (MQ=255)                                                                                                                                gCATATCGGTAACGACAAGAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGCCACTCTCCTGCGGTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCttgtt               >  1:527316/1‑151 (MQ=255)                                                                                                                                 cATATCGGTAACGACAAGAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGCCACTCTCCTGCGGTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCttgttg              <  2:493368/151‑1 (MQ=255)                                                                                                                                 cATATCGGTAACGACAAGAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGCCACTCTCCTGCGGTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCttgttg              >  1:640404/1‑151 (MQ=255)                                                                                                                                  atatCGGTAACGACAAGAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGCCACTCTCCTGCGGTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGCGTCGGCGAGGGTACGCGCCACTGCCAGTGCCttgttg              >  1:865528/1‑150 (MQ=255)                                                                                                                                       ggTAACGACAAGAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGCCACTCTCCTGCGGTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGc        <  1:905966/151‑1 (MQ=255)                                                                                                                                        gTAACGACAAGAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGCCACTCTCCTGCGGTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGcc       >  2:488382/1‑151 (MQ=255)                                                                                                                                            cGACAAGAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGCCACTCTCCTGCGGTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCAtc   >  2:857513/1‑151 (MQ=255)                                                                                                                                             gACAAGAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGCCACTCTCCTGCGGTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCAtct  >  2:466780/1‑151 (MQ=255)                                                                                                                                                  |                                                                                                                                                  AAAGCCTGCGCCAGAAAACCGCGATTGATAGCGACATTTTCTTCGCCATTCAGCGCTTTAAGGATGTCATCACCTTTAAATGGCTGACCAATACGCTGGGCTTCAGGATGCGAGTAGCGAAGACTTTGCATATCGGTAACGACAATAAACAGCAGATCGTTGCGTTTGCGTACGGCTTCCGCGATGGCCTGGATGCCACTCTCCTGCGGTTTTTTCTGCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCT  >  NC_000913/4351226‑4351516

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑