Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,399,742	A→C	D164A (GAT→GCT)	miaA →	delta(2)‑isopentenylpyrophosphate tRNA‑adenosine transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,399,742	0	A	C	100.0%	169.7 / NA	45	D164A (GAT→GCT)	miaA	delta(2)‑isopentenylpyrophosphate tRNA‑adenosine transferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (21/24);  total (21/24)

TGCTGGAAGGGTTGTCGCCGCTACCGTCGGCAGACCCGGAAGTACGGGCCAGAATTGAGCAACAGGCGGCAGAGCAAGGTTGGGAGTCATTGCATCGTCAACTTCAGGAGGTAGATCCGGTTGCGGCAGCAAGGATTCATCCAAATGATCCACAAAGGCTTTCCCGGGCACTGGAAGTTTTTTTCATTTCGGGTAAAACTTTAACGGAACTGACGCAAACGTCAGGAGACGCTCTACCGTATCAGGTGCATCAGTTCGCCATCGCCCCGGCGAGCCGTGAACTGCTCCATCAAC  >  NC_000913/4399595‑4399888                                                                                                                                                    |                                                                                                                                                   tGCTGGAAGGGTTGTCGCCGCTACCGTCGGCAGACCCGGAAGTACGGGCCAGAATTGAGCAACAGGCGGCAGAGCAAGGTTGGGAGTCATTGCATCGTCAACTTCAGGAGGTAGATCCGGTTGCGGCAGCAAGGATTCATCCAAATGCTcc                                                                                                                                                 >  1:697290/1‑151 (MQ=255)    tGGAAGGGTTGTCGCCGCTACCGTCGGCAGACCCGGAAGTACGGGCCAGAATTGAGCAACAGGCGGCAGAGCAAGGTTGGGAGTCATTGCATCGTCAACTTCAGGAGGTAGATCCGGTTGCGGCAGCAAGGATTCATCCAAATGCTCcac                                                                                                                                               >  2:312140/1‑150 (MQ=255)     ggAAGGGTTGTCGCCGCTACCGTCGGCAGACCCGGAAGTACGGGCCAGAATTGAGCAACAGGCGGCAGAGCAAGGTTGGGAGTCATTGCATCGTCAACTTCAGGAGGTAGATCCGGTTGCGGCAGCAAGGATTCATCCAAATGCTCcaca                                                                                                                                              <  2:902796/150‑1 (MQ=255)     ggAAGGGTTGTCGCCGCTACCGTCGGCAGACCCGGAAGTACGGGCCAGAATTGAGCAACAGGCGGCAGAGCAAGGTTGGGAGTCATTGCATCGTCAACTTCAGGAGGTAGATCCGGTTGCGGCAGCAAGGATTCATCCAAATGCTCCACaa                                                                                                                                             <  1:519239/151‑1 (MQ=255)     ggAAGGGTTGTCGCCGCTACCGTCGGCAGACCCGGAAGTACGGGCCAGAATTGAGCAACAGGCGGCAGAGCAAGGTTGGGAGTCATTGCATAGTCAACTTCAGGAGGTAGATCCGGTTGCGGCAGCAAGGATTCATCCAAATGCTCcaca                                                                                                                                              >  1:119607/1‑150 (MQ=255)     ggAAGGGTTGTCGCCCCTGCCGTCGGCAGAACCGGAAGTACGGGCCAGAATTGAGCAACAGGCGGCAGAGCAAGGGTGGGAGTCATTGCATCGTCAACTTCAGGAGGTAGATCCGGTTGCGGCAGCAAGGATTCATCCAAATGCTCCACaa                                                                                                                                             <  1:920410/151‑1 (MQ=255)           gTTGTCGCCGCTACCGTCGGCAGACCCGGAAGTACGGGCCAGAATTGAGCAACAGGCGGCAGAGCAAGGTTGGGAGTCATTGCATCGTCAACTTCAGGAGGTAGATCCGGTTGCGGCAGCAAGGATTCATCCAAATGCTCCACAAAGGCtt                                                                                                                                       >  1:737977/1‑151 (MQ=255)                 gccgcTACCGTCGGCAGACCCGGAAGTACGGGCCAGAATTGAGCAACAGGCGGCAGAGCAAGGTTGGGAGTCATTGCATCGTCAACTTCAGGAGGTAGATCCGGTTGCGGCAGCAAGGATTCATCCAAATGCTCCACAAAGGCTTTCCCgg                                                                                                                                 <  1:62923/151‑1 (MQ=255)                      tACCGTCGGCAGACCCGGAAGTACGGGCCAGAATTGAGCAACAGGCGGCAGAGCAAGGTTGGGAGTCATTGCATCGTCAACTTCAGGAGGTAGATCCGGTTGCGGCAGCAAGGATTCATCCAAATGCTCCACAAAGGCTTTCCCGGGCACt                                                                                                                            >  2:700144/1‑151 (MQ=255)                       aCCGTCGGCAGACCCGGAAGTACGGGCCAGAATTGAGCAACAGGCGGCAGAGCAAGGTTGGGAGTCATTGCATCGTCAACTTCAGGAGGTAGATCCGGTTGCGGCAGCAAGGATTCATCCAAATGCTCCACAAAGGCTTTCCCGGGCAc                                                                                                                             <  1:765227/149‑1 (MQ=255)                         cGTCGGCAGACCCGGAAGTACGGGCCAGAATTGAGCAACAGGCGGCAGAGCAAGGTTGGGAGTCATTGCATCGTCAACTTCAGGAGGTAGATCCGGTTGCGGCAGCAAGGATTCATCCAAATGCTCCACAAAGGCTTTCCCGGGCACTgg                                                                                                                          >  2:251661/1‑150 (MQ=255)                          gTCGGCAGACCCGGAAGTACGGGCCAGAATTGAGCAACAGGCGGCAGAGCAAGGTTGGGAGTCATTGCATCGTCAACTTCAGGAGGTAGATCCGGTTGCGGCAGCAAGGATTCATCCAAATGCTCCACAAAGGCTTTCCCGGGCACTGGaa                                                                                                                        <  1:798225/151‑1 (MQ=255)                                   cccGGAAGTACGGGCCAGAATTGAGCAACAGGCGGCAGAGCAAGGTTGGGAGTCATTGCATCGTCAACTTCAGGAGGTAGATCCGGTTGCGGCAGCAAGGATTCATCCAAATGCTCCACAAAGGCTTTCCCGGGCACTGGAAGTTTTTTTc                                                                                                               <  2:812612/151‑1 (MQ=255)                                   cccGGAAGTACGGGCCAGAATTGAGCAACAGGCGGCAGAGCAAGGTTGGGAGTCATTGCATCGTCAACTTCAGGAGGTAGATCCGGTTGCGGCAGCAAGGATTCATCCAAATGCTCCACAAAGGCTTTCCCGGGCACTGGAAGTTTTTTTc                                                                                                               >  2:508639/1‑151 (MQ=255)                                            aCGGGCCAGAATTGAGCAACAGGCGGCAGAGCAAGGTTGGGAGTCATTGCATCGTCAACTTCAGGAGGTAGATCCGGTTGCGGCAGCAAGGATTCATCCAAATGCTCCACAAAGGCTTTCCCGGGCACTGGAAGTTTTTTTCATTTCGGGt                                                                                                      <  1:366624/151‑1 (MQ=255)                                             cGGGCCAGAATTGAGCAACAGGCGGCAGAGCAAGGTTGGGAGTCATTGCATCGTCAACTTCAGGAGGTAGATCCGGTTGCGGCAGCAAGGATTCATCCAAATGCTCCACAAAGGCTTTCCCGGGCACTGGAAGTTTTTTTCATTTCGGGt                                                                                                      >  1:815219/1‑150 (MQ=255)                                                 ccAGAATTGAGCAACAGGCGGCAGAGCAAGGTTGGGAGTCATTGCATCGTCAACTTCAGGAGGTAGATCCGGTTGCGGCAGCAAGGATTCATCCAAATGCTCCACAAAGGCTTTCCCGGGCACTGGAAGTTTTTTTCATTTCGGGTAAAAc                                                                                                 >  2:865916/1‑151 (MQ=255)                                                   aGAATTGAGCAACAGGCGGCAGAGCAAGGTTGGGAGTCATTGCATCGTCAACTTCAGGAGGTAGATCCGGTTGCGGCAGCAAGGATTCATCCAAATGCTCCACAAAGGCTTTCCCGGGCACTGGAAGTTTTTTTCATTTCGGGTAAAACtt                                                                                               <  1:921015/151‑1 (MQ=255)                                                   aGAATTGAGCAACAGGCGGCAGAGCAAGGTTGGGAGTCATTGCATCGTCAACTTCAGGAGGTAGATCCGGTTGCGGCAGCAAGGATTCATCCAAATGCTCCACAAAGGCTTTCCCGGGCACTGGAAGTTTTTTTCATTTCGGGTAAAACtt                                                                                               >  1:822673/1‑151 (MQ=255)                                                               cAGGCGGCAGAGCAAGGTTGGGAGTCATTGCATCGTCAACTTCAGGAGGTAGATCCGGTTGCGGCAGCAAGGATTCATCCAAATGCTCCACAAAGGCTTTCCCGGGCACTGGAAGTTTTTTTCATTTCGGGTAAAACTTTAACGGAACTGa                                                                                   <  2:761115/151‑1 (MQ=255)                                                                 ggcggcAGAGCAAGGTTGGGAGTCATTGCATCGTCAACTTCAGGAGGTAGATCCGGTTGCGGCAGCAAGGATTCATCCAAATGCTCCACAAAGGCTTTCCCGGGCACTGGAAGTTTTTTTCATTTCGGGTAAAACTTTAACGGAACTGACg                                                                                 <  2:133407/151‑1 (MQ=255)                                                                 ggcggcAGAGCAAGGTTGGGAGTCATTGCATCGTCAACTTCAGGAGGTAGATCCGGTTGCGGCAGCAAGGATTCATCCAAATGCTCCACAAAGGCTTTCCCGGGCACTGGAAGTTTTTTTCATTTCGGGTAAAACTTTAACGGAACTGACg                                                                                 <  1:516191/151‑1 (MQ=255)                                                                     gcAGAGCAAGGTTGGGAGTCATTGCATCGTCAACTTCAGGAGGTAGATCCGGTTGCGGCAGCAAGGATTCATCCAAATGCTCCACAAAGGCTTTCCCGGGCACTGGAAGTTTTTTTCATTTCGGGTAAAACTTTAACGGAACTGACGCaa                                                                              >  2:316954/1‑150 (MQ=255)                                                                          gCAAGGTTGGGAGTCATTGCATCGTCAACTTCAGGAGGTAGATCCGGTTGCGGCAGCAAGGATTCATCCAAATGCTCCACAAAGGCTTTCCCGGGCACTGGAAGTTTTTTTCATTTCGGGTAAAACTTTAACGGAACTGACGCAAACGTCa                                                                        <  1:438762/151‑1 (MQ=255)                                                                          gCAAGGTTGGGAGTCATTGCATCGTCAACTTCAGGAGGTAGATCCGGTTGCGGCAGCAAGGATTCATCCAAATGCTCCACAAAGGCTTTCCCGGGCACTGGAAGTTTTTTTCATTTCGGGTAAAACTTTAACGGAACTGACGCAAACGTCa                                                                        <  2:882205/151‑1 (MQ=255)                                                                                  gggAGTCATTGCATCGTCAACTTCAGGAGGTAGATCCGGTTGCGGCAGCAAGGATTCATCCAAATGCTCCACAAAGGCTTTCCCGGGCACTGGAAGTTTTTTTCATTTCGGGTAAAACTTTAACGGAACTGACGCAAACGTCAGGAGACGc                                                                <  1:188444/151‑1 (MQ=255)                                                                                         aTTGCATCGTCAACTTCAGGAGGTAGATCCGGTTGCGGCAGCAAGGATTCATCCAAATGCTCCACAAAGGCTTTCCCGGGCACTGGAAGTTTTTTTCATTTCGGGTAAAACTTTAACGGAACTGACGCAAACGTCAGGAGACGCTCTACCg                                                         >  2:173646/1‑151 (MQ=255)                                                                                         aTTGCATCGTCAACTTCAGGAGGTAGATCCGGTTGCGGCAGCAAGGATTCATCCAAATGCTCCACAAAGGCTTTCCCGGGCACTGGAAGTTTTTTTCATTTCGGGTAAAACTTTAACGGAACTGACGCAAACGTCAGGAGACGCTCTACCg                                                         >  2:765281/1‑151 (MQ=255)                                                                                           tGCATCGTCAACTTCAGGAGGTAGATCCGGTTGCGGCAGCAAGGATTCATCCAAATGCTCCACAAAGGCTTTCCCGGGCACTGGAAGTTTTTTTCATTTCGGGTAAAACTTTAACGGAACTGACGCAAACGTCAGGAGACGCTCTACCGt                                                        <  1:477969/150‑1 (MQ=255)                                                                                                cGTCAACTTCAGGAGGTAGATCCGGTTGCGGCAGCAAGGATTCATCCAAATGCTCCACAAAGGCTTTCCCGGGCACTGGAAGTTTTTTTCATTTCGGGTAAAACTTTAACGGAACTGACGCAAACGTCAGGAGACGCTCTACCGTATCAgg                                                  >  1:527522/1‑151 (MQ=255)                                                                                                cGTCAACTTCAGGAGGTAGATCCGGTTGCGGCAGCAAGGATTCATCCAAATGCTCCACAAAGGCTTTCCCGGGCACTGGAAGTTTTTTTCATTTCGGGTAAAACTTTAACGGAACTGACGCAAACGTCAGGAGACGCTCTACCGTATCAg                                                   <  2:709820/150‑1 (MQ=255)                                                                                                         cAGGAGGTAGATCCGGTTGCGGCAGCAAGGATTCATCCAAATGCTCCACAAAGGCTTTCCCGGGCACTGGAAGTTTTTTTCATTTCGGGTAAAACTTTAACGGAACTGACGCAAACGTCAGGAGACGCTCTACCGTATCAGGTGCATCAg                                          <  2:119609/150‑1 (MQ=255)                                                                                                            gaggTAGATCCGGTTGCGGCAGCAAGGATTCATCCAAATGCTCCACAAAGGCTTTCCCGGGCACTGGAAGTTTTTTTCATTTCGGGTAAAACTTTAACGGAACTGACGCAAACGTCAGGAGACGCTCTACCGTATCAGGTGCATCAGTTCg                                      <  2:340811/151‑1 (MQ=255)                                                                                                              ggTAGATCCGGTTGCGGCAGCAAGGATTCATCCAAATGCTCCACAAAGGCTTTCCCGGGCACTGGAAGTTTTTTTCATTTCGGGTAAAACTTTAACGGAACTGACGCAAACGTCAGGAGACGCTCTACCGTATCAGGTGCATCAGTTCGcc                                    >  2:310661/1‑151 (MQ=255)                                                                                                                 aGATCCGGTTGCGGCAGCAAGGATTCATCCAAATGCTCCACAAAGGCTTTCCCGGGCACTGGAAGTTTTTTTCATTTCGGGTAAAACTTTAACGGAACTGACGCAAACGTCAGGAGACGCTCTACCGTATCAGGTGCATCAGTTCGCCATc                                 >  1:706548/1‑151 (MQ=255)                                                                                                                 aGATCCGGTTGCGGCAGCAAGGATTCATCCAAATGCTCCACAAAGGCTTTCCCGGGCACTGGAAGTTTTTTTCATTTCGGGTAAAACTTTAACGGAACTGACGCAAACGTCAGGAGACGCTCTACCGTATCAGGTGCATCAGTTCGCCATc                                 >  1:660473/1‑151 (MQ=255)                                                                                                                   aTCCGGTTGCGGCAGCAAGGATTCATCCAAATGCTCCACAAAGGCTTTCCCGGGCACTGGAAGTTTTTTTCATTTCGGGTAAAACTTTAACGCAACTGACGCAAACGTCAGGAGACGCTCTACCGTATCAGGTGCATCAGTTCGCCATCGc                               <  2:83198/151‑1 (MQ=255)                                                                                                                       ggTTGCGGCAGCAAGGATTCATCCAAATGCTCCACAAAGGCTTTCCCGGGCACTGGAAGTTTTTTTCATTTCGGGTAAAACTTTAACGGAACTGACGCAAACGTCAGGAGACGCTCTACCGTATCAGGTGCATCAGTTCGCCATCGCCCCg                           <  1:57973/151‑1 (MQ=255)                                                                                                                          tGCGGCAGCAAGGATTCATCCAAATGCTCCACAAAGGCTTTCCCGGGCACTGGAAGTTTTTTTCATTTCGGGTAAAACTTTAACGGAACTGACGCAAACGTCAGGAGACGCTCTACCGTATCAGGTGCATCAGTTCGCCATCGCCCCGGc                         <  1:564589/150‑1 (MQ=255)                                                                                                                          tGCGGCAGCAAGGATTCATCCAAATGCTCCACAAAGGCTTTCCCGGGCACTGGAAGTTTTTTTCATTTCGGGTAAAACTTTAACGGAACTGACGCAAACGTCAGGAGACGCTCTACCGTATCAGGTGCATCAGTTCGCCATCGCCCCGGCg                        >  2:356194/1‑151 (MQ=255)                                                                                                                                        ttCATCCAAATGCTCCACAAAGGCTTTCCCGGGCACTGGAAGTTTTTTTCATTTCGGGTAAAACTTTAACGGAACTGACGCAAACGTCAGGAGACGCTCTACCGTATCAGGTGCATCAGTTCGCCATCGCCCCGGCGAGCCGTGAACTGCt          >  1:842024/1‑151 (MQ=255)                                                                                                                                             ccAAATGCTCCACAAAGGCTTTCCCGGGCACTGGAAGTTTTTTTCATTTCGGGTAAAACTTTAACGGAACTGACGCAAACGTCAGGAGACGCTCTACCGTATCAGGTGCATCAGTTCGCCATCGCCCCGGCGAGCCGTGAACTGCTCCAt      <  1:83329/150‑1 (MQ=255)                                                                                                                                              cAAATGCTCCACAAAGGCTTTCCCGGGCACTGGAAGTTTTTTTCATTTCGGGTAAAACTTTAACGGAACTGACGCAAACGTCAGGAGACGCTCTACCGTATCAGGTGCATCAGTTCGCCATCGCCCCGGCGAGCCGTGAACTGCTCCATc     >  1:344678/1‑150 (MQ=255)                                                                                                                                              cAAATGCTCCACAAAGGCTTTCCCGGGCACTGGAAGTTTTTTTCATTTCGGGTAAAACTTTAACGGAACTGACGCAAACGTCAGGAGACGCTCTACCGTATCAGGTGCATCAGTTCGCCATCGCCCCGGCGAGCCGTGAACTGCTCCATCa    <  2:266289/151‑1 (MQ=255)                                                                                                                                                aaTGCTCCACAAAGGCTTTCCCGGGCACTGGAAGTTTTTTTCATTTCGGGTAAAACTTTAACGGAACTGACGCAAACGTCAGGAGACGCTCTACCGTATCAGGTGCATCAGTTCGCCATCGCCCCGGCGAGCCGTGAACTGCTCCATCAAc  >  2:286111/1‑151 (MQ=255)                                                                                                                                                    |                                                                                                                                                   TGCTGGAAGGGTTGTCGCCGCTACCGTCGGCAGACCCGGAAGTACGGGCCAGAATTGAGCAACAGGCGGCAGAGCAAGGTTGGGAGTCATTGCATCGTCAACTTCAGGAGGTAGATCCGGTTGCGGCAGCAAGGATTCATCCAAATGATCCACAAAGGCTTTCCCGGGCACTGGAAGTTTTTTTCATTTCGGGTAAAACTTTAACGGAACTGACGCAAACGTCAGGAGACGCTCTACCGTATCAGGTGCATCAGTTCGCCATCGCCCCGGCGAGCCGTGAACTGCTCCATCAAC  >  NC_000913/4399595‑4399888

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑