Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,424,756	A→C	L12L (CTT→CTG)	yjfY ←	YhcN family protein, periplasmic

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,424,756	0	A	C	100.0%	268.5 / NA	70	L12L (CTT→CTG)	yjfY	YhcN family protein, periplasmic
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (34/36);  total (34/36)

TGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGCCTGGTGGTTATTAATTTCAATGGCTGCCCATGTATTTGCACTTAGCAAAAGCACAGCCAGAAGGGCTAAAACACGACTGAACATAGATACCTCCTCGACGGCTGACTTTGTGTGCTCTCCTCTGTGATGATCTTCTGATTTAATTTTAATCAATGATAAAGAAGTTGATGGTGACCATTTCTGATGCAGTT  >  NC_000913/4424609‑4424898                                                                                                                                                    |                                                                                                                                               tGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGCCTGGTGGTTATTAATTTCAATGGCTGCCCATGTATTTGCACTTAGCAACAGc                                                                                                                                             >  2:199638/1‑151 (MQ=255) tGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGCCTGGTGGTTATTAATTTCAATGGCTGCCCATGTATTTGCACTTAGCAACAGc                                                                                                                                             >  1:336576/1‑151 (MQ=255)        tcATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGCCTGGTGGTTATTAATTTCAATGGCTGCCCATGTATTTGCACTTAGCAacagcacagc                                                                                                                                        <  1:836288/149‑1 (MQ=255)         cATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGCCTGGTGGTTATTAATTTCAATGGCTGCCCATGTATTTGCACTTAGCAACAGCACAGCCa                                                                                                                                      <  2:834983/150‑1 (MQ=255)          aTTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGCCTGGTGGTTATTAATTTCAATGGCTGCCCATGTATTTGCACTTAGCAACAGCACAGCCAg                                                                                                                                     <  1:182990/150‑1 (MQ=255)           tttAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGCCTGGTGGTTATTAATTTCAATGGCTGCCCATGTATTTGCACTTAGCAACAGCACAGCCAGa                                                                                                                                    <  1:514302/150‑1 (MQ=255)             tAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGCCTGGTGGTTATTAATTTCAATGGCTGCCCATGTATTTGCACTTAGCAACAGCACAGCCAGAAgg                                                                                                                                 >  2:318872/1‑151 (MQ=255)             tAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGCCTGGTGGTTATTAATTTCAATGGCTGCCCATGTATTTGCACTTAGCAACAGCACAGCCAGAAg                                                                                                                                  <  1:248293/150‑1 (MQ=255)                 gCCTGACGTGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGCCTGGTGGTTATTAATTTCAATGGCTGCCCATGTATTTGCACTTAGCAACAGCACAGCCAGAAGGGCt                                                                                                                              >  2:273883/1‑150 (MQ=255)                 gCCTGACGTGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGCCTGGTGGTTATTAATTTCAATGGCTGCCCATGTATTTGCACTTAGCAACAGCACAGCCAGAAGGGCt                                                                                                                              >  2:613178/1‑150 (MQ=255)                    tGACGTGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGCCTGGTGGTTATTAATTTCAATGGCTGCCCATGTATTTGCACTTAGCAACAGCACAGCCAGAAGGGCTaaa                                                                                                                           >  2:81878/1‑150 (MQ=255)                     gACGTGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGCCTGGTGGTTATTAATTTCAATGGCTGCCCATGTATTTGCACTTAGCAACAGCACAGCCAGAAGGGCTAAAac                                                                                                                         <  2:323892/151‑1 (MQ=255)                      aCGTGCTTCGCTTTCAGTGGCGACATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGCCTGGTGGATATTAATTTCAATGGCTGCCCATGTATTTGCACTTAGCAACAGAACAGCCAGAAGGGCTaaaa                                                                                                                          >  1:888027/1‑149 (MQ=255)                      aCGTGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACACCTAAGCTTTGCACATCGTCCATATTTCTGGCCTGGTGGTTATTAATTTCAATGGCTGCCCATGTATTTGCACTTAGCAACAGCACAGCCAGAAGGGCTAAAac                                                                                                                         >  2:567561/1‑150 (MQ=255)                          gCTTCGCTTTCAGTGGCGAAATTACGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGCCTGGTGGTTATTAATTTCAATGGCTGCCCATGTATTTGCACTTAGCAACAGCACAGCCAGAAGGGCTAAAACACGAc                                                                                                                    >  2:24743/1‑151 (MQ=255)                             tCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGCCTGGTGGTTATTAATTTCAATGGCTGCCCATGTATTTGCACTTAGCAACAGCACAGCCAGAAGGGCTAAAACACGACt                                                                                                                   <  2:685491/149‑1 (MQ=255)                              cGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGCCTGGTGGTTATTAATTTCAATGGCTGCCCATGTATTTGCACTTAGCAACAGCACAGCCAGAAGGGCTAAAACACGACTGa                                                                                                                 >  1:53647/1‑150 (MQ=255)                                cTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGCCTGGTGGTTATTAATTTCAATGGCTGCCCATGTATTTGCACTTAGCAACAGCACAGCCAGAAGGGCTAAAACACGACTGAAc                                                                                                               >  2:557199/1‑150 (MQ=255)                                   tCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGCCTGGTGGTTATTAATTTCAATGGCTGCCCATGTATTTGCACTTAGCAACAGCACAGCCAGAAGGGCTAAAACACGACTGAACATa                                                                                                            <  1:743240/150‑1 (MQ=255)                                    cAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGCCTGGTGGTTATTAATTTCAATGGCTGCCCATGTATTTGCACTTAGCAACAGCACAGCCAGAAGGGCTAAAACACGACTGAACAt                                                                                                             >  1:581309/1‑148 (MQ=255)                                    cAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGCCTGGTGGTTATTAATTTCAATGGCTGCCCATGTATTTGCACTTAGCAACAGCACAGCCAGAAGGGCTAAAACACGACTGAACATAg                                                                                                           <  2:134419/150‑1 (MQ=255)                                        ggCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGCCTGGTGGTTATTAATTTCAATGGCTGCCCATGTATTTGCACTTAGCAACAGCACAGCCAGAAGGGCTAAAACACGACTGAACATAGATAcc                                                                                                      >  2:53960/1‑151 (MQ=255)                                        ggCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGCCTGGTGGTTATTAATTTCAATGGCTGCCCATGTATTTGCACTTAGCAACAGCACAGCCAGAAGGGCTAAAACACGACTGAACATAGATAc                                                                                                       <  1:202545/150‑1 (MQ=255)                                              aTTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGCCTGGTGGTTATTAATTTCAATGGCTGCCCATGTATTTGCACTTAGCAACAGCACAGCCAGAAGGGCTAAAACACGACTGAACATAGATACCTCCTCg                                                                                                <  2:417390/151‑1 (MQ=255)                                                  tgattgatATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGCCTGGTGGTTATTAATTTCAATGGCTGCCCATGTATTTGCACTTAGCAACAGCACAGCCAGAAGGGCTAAAACACGACTGAACATAGATACCTCCTCGACg                                                                                             <  1:588250/150‑1 (MQ=255)                                                          ataAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGCCTGGTGGTTATTAATTTCAATGGCTGCCCATGTATTTGCACTTAGCAACAGCACAGCCAGAAGGGCTAAAACACGACTGAACATAGATACCTCCTCGACGGCTGACtt                                                                                     <  2:61360/150‑1 (MQ=255)                                                           taAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGCCTGGTGGTTATTAATTTCAATGGCTGCCCATGTATTTGCACTTAGCAACAGCACAGCCAGAAGGGCTAAAACACGACTGAACATAGATACCTCCTCGACGGCTGACTTtg                                                                                   >  2:565277/1‑151 (MQ=255)                                                             aaTCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGCCTGGTGGTTATTAATTTCAATGGCTGCCCATGTATTTGCACTTAGCAACAGCACAGCCAGAAGGGCTAAAACACGACTGAACATAGATACCTCCTCGACGGCTGACTTtgtg                                                                                 <  1:834441/151‑1 (MQ=255)                                                              aTCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGCCTGGTGGTTATTAATTTCAATGGCTGCCCATGTATTTGCACTTAGCAACAGCACAGCCAGAAGGGCTAAAACACGACTGAACATAGATACCTCCTCGACGGCTGACTTtgtgt                                                                                <  1:448960/151‑1 (MQ=255)                                                                    ccTAAGCTTTGCACATCGTCCATATTTCTGGCCTGGTGGTTATTAATTTCAATGGCTGCCCATGTATTTGCACTTAGCAACAGCACAGCCAGAAGGGCTAAAACACGACTGAACATAGATACCTCCTCGACGGCTGACTTTGTGTGCtctc                                                                          <  2:617750/151‑1 (MQ=255)                                                                     cTAAGCTTTGCACATCGTCCATATTTCTGGCCTGGTGGTTATTAATTTCAATGGCTGCCCATGTATTTGCACTTAGCAACAGCACAGCCAGAAGGGCTAAAACACGACTGAACATAGATACCTCCTCGACGGCTGACTTTGTGTGCTctcc                                                                         >  1:59770/1‑151 (MQ=255)                                                                     cTAAGCTTTGCACATCGTCCATATTTCTGGCCTGGTGGTTATTAATTTCAATGGCTGCCCATGTATTTGCACTTAGCAACAGCACAGCCAGAAGGGCTAAAACACGACTGAACATAGATACCTCCTCGACGGCTGACTTTGTGTGCTctcc                                                                         >  1:18863/1‑151 (MQ=255)                                                                      tAAGCTTTGCACATCGTCCATATTTCTGGCCTGGTGGTTATTAATTTCAATGGCTGCCCATGTATTTGCACTTAGCAACAGCACAGCCAGAAGGGCTAAAACACGACTGAACATAGATACCTCCTCGACGGCTGACTTTGTGTGCTctcct                                                                        >  2:766635/1‑151 (MQ=255)                                                                       aaGCTTTGCACATCGTCCATATTTCTGGCCTGGTGGTTATTAATTTCAATGGCTGCCCATGTATTTGCACTTAGCAACAGCACAGCCAGAAGGGCTAAAACACGACTGAACATAGATACCTCCTCGACGGCTGACTTTGTGTGCTctcc                                                                         <  2:165817/149‑1 (MQ=255)                                                                        aGCTTTGCACATCGTCCATATTTCTGGCCTGGTGGTTATTAATTTCAATGGCTGCCCATGTATTTGCACTTAGCAACAGCACAGCCAGAAGGGCTAAAACACGACTGAACATAGATACCTCCTCGACGGCTGACTTTGTGTGCTCTCctct                                                                      <  1:186371/151‑1 (MQ=255)                                                                         gCTTTGCACATCGTCCATATTTCTGGCCTGGTGGTTATTAATTTCAATGGCTGCCCATGTATTTGCACTTAGCAACAGCACAGCCAGAAGGGCTAAAACACGACTGAACATAGATACCTCCTCGACGGCTGACTTTGTGTGCTCTCCTCtg                                                                     >  2:740788/1‑151 (MQ=255)                                                                           tttGCACATCGTCCATATTTCTGGCCTGGTGGTTATTAATTTCAATGGCTGCCCATGTATTTGCACTTAGCAACAGCACAGCCAGAAGGGCTAAAACACGACTGAACATAGATACCTCCTCGACGGCTGACTTTGTGTGCTCTCCTCtgtg                                                                   >  1:885676/1‑151 (MQ=255)                                                                           tttGCACATCGTCCATATTTCTGGCCTGGTGGTTATTAATTTCAATGGCTGCCCATGTATTTGCACTTAGCAACAGCACAGCCAGAAGGGCTAAAACACGACTGAACATAGATACCTCCTCGACGGCTGACTTTGTGTGCTCTCCTCtgtg                                                                   >  2:276017/1‑151 (MQ=255)                                                                           tttGCACATCGTCCATATTTCTGGCCTGGTGGTTATTAATTTCAATGGCTGCCCATGTATTTGCACTTAGCAACAGCACAGCCAGAAGGGCTAAAACACGACTGAACATAGATACCTCCTCGACGGCTGACTTTGTGTGCTCTCCTCtgtg                                                                   >  2:412086/1‑151 (MQ=255)                                                                            ttGCACATCGTCCATATTTCTGGCCTGGTGGTTATTAATTTCAATGGCTGCCCATGTATTTGCACTTAGCAACAGCACAGCCAGAAGGGCTAAAACACGACTGAACATAGATACCTCCTCGACGGCTGACTTTGTGTGCTCTCCTCtgt                                                                    <  2:150983/149‑1 (MQ=255)                                                                            ttGCACATCGTCCATATTTCTGGCCTGGTGGTTATTAATTTCAATGGCTGCCCATGTATTTGCACTTAGCAACAGCACAGCCAGAAGGGCTAAAACACGACTGAACATAGATACCTCCTCGACGGCTGACTTTGTGTGCTCTCCTCtgt                                                                    <  1:607967/149‑1 (MQ=255)                                                                             tGCACATCGTCCATATTTCTGGCCTGGTGGTTATTAATTTCAATGGCTGCCCATGTATTTGCACTTAGCAACAGCACAGCCAGAAGGGCTAAAACACGACTGAACATAGATACCTCCTCGACGGCTGACTTTGTGTGCTCTCCTCTGtgat                                                                 >  1:53184/1‑151 (MQ=255)                                                                              gCACATCGTCCATATTTCTGGCCTGGTGGTTATTAATTTCAATGGCTGCCCATGTATTTGCACTTAGCAACAGCACAGCCAGAAGGGCTAAAACACGACTGAACATAGATACCTCCTCGACGGCTGACTTTGTGTGCTCTCCTCTGtgatg                                                                <  1:645749/151‑1 (MQ=255)                                                                              gCACATCGTCCATATTTCTGGCCTGGTGGTTATTAATTTCAATGGCTGCCCATGTATTTGCACTTAGCAACAGCACAGCCAGAAGGGCTAAAACACGACTGAACATAGATACCTCCTCGACGGCTGACTTTGTGTGCTCTCCTCTGtgatg                                                                <  1:500792/151‑1 (MQ=255)                                                                               cacaTCGTCCATATTTCTGGCCTGGTGGTTATTAATTTCAATGGCTGCCCATGTATTTGCACTTAGCAACAGCACAGCCAGAAGGGCTAAAACACGACTGAACATAGATACCTCCTCGACGGCTGACTTTGTGTGCTCTCCTCTGtgatg                                                                >  1:148858/1‑150 (MQ=255)                                                                                  aTCGTCCATATTTCTGGCCTGGTGGTTATTAATTTCAATGGCTGCCCATGTATTTGCACTTAGCAACAGCACAGCCAGAAGGGCTAAAACACGACTGAACATAGATACCTCCTCGACGGCTGACTTTGTGTGCTCTCCTCTGTGATGAtct                                                            <  1:231154/151‑1 (MQ=255)                                                                                   tCGTCCATATTTCTGGCCTGGTGGTTATTAATTTCAATGGCTGCCCATGTATTTGCACTTAGCAACAGCACAGCCAGAAGGGCTAAAACACGACTGAACATAGATACCTCCTCGACGGCTGACTTTGTGTGCTCTCCTCTGTGATGAtctt                                                           >  2:405579/1‑151 (MQ=255)                                                                                    cGTCCATATTTCTGGCCTGGTGGTTATTAATTTCAATGGCTGCCCATGTATTTGCACTTAGCAACAGCACAGCCAGAAGGGCTAAAACACGACTGAACATAGATACCTCCTCGACGGCTGACTTTGTGTGCTCTCCTCTGTGATGAtcttc                                                          <  1:815253/151‑1 (MQ=255)                                                                                     gTCCATATTTCTGGCCTGGTGGTTATTAATTTCAATGGCTGCCCATGTATTTGCACTTAGCAACAGCACAGCCAGAAGGGCTAAAACACGACTGAACATAGATACCTCCTCGACGGCTGACTTTGTGTGCTCTCCTCTGTGATGAtcttct                                                         <  1:105162/151‑1 (MQ=255)                                                                                           atTTCTGGCCTGGTGGTTATTAATTTCAATGGCTGCCCATGTATTTGCACTTAGCAACAGCACAGCCAGAAGGGCTAAAACACGACTGAACATAGATACCTCCTCGACGGCTGACTTTGTGTGCTCTCCTCTGTGATGATCTTCTGAttt                                                    >  1:218000/1‑150 (MQ=255)                                                                                             ttCTGGCCTGGTGGTTATTAATTTCAATGGCTGCCCATGTATTTGCACTTAGCAACAGCACAGCCAGAAGGGCTAAAACACGACTGAACATAGATACCTCCTCGACGGCTGACTTTGTGTGCTCTCCTCTGTGATGATCTTCTGAtttaa                                                  <  2:90478/150‑1 (MQ=255)                                                                                                      ggtggtTATTAATTTCAATGGCTGCCCATGTATTTGCACTTAGCAACAGCACAGCCAGAAGGGCTAAAACACGACTGAACATAGATACCTCCTCGACGGCTGACTTTGTGTGCTCTCCTCTGTGATGATCTTCTGATTTAATTTTAATCa                                         <  2:232479/150‑1 (MQ=255)                                                                                                       gtggtTATTAATTTCAATGGCTGCCCATGTATTTGCACTTAGCAACAGCACAGCCAGAAGGGCTAAAACACGACTGAACATAGATACCTCCTCGACGGCTGACTTTGTGTGCTCTCCTCTGTGATGATCTTCTGATTTAATTTTAATCAAt                                       <  2:160617/151‑1 (MQ=255)                                                                                                        tggtTATTAATTTCAATGGCTGCCCATGTATTTGCACTTAGCAACAGCACAGCCAGAAGGGCTAAAACACGACTGAACATAGATACCTCCTCGACGGCTGACTTTGTGTGCTCTCCTCTGTGATGATCTTCTGATTTAATTTTAATCAATg                                      >  1:385834/1‑151 (MQ=255)                                                                                                         ggtTATTAATTTCAATGGCTGCCCATGTATTTGCACTTAGCAACAGCACAGCCAGAAGGGCTAAAACACTACTGAACATAGATACCTCCTCGACGGCTGACTTTGTGTGCTCTCCTCTGTGATGATCTTCTGATTTAATTTTAATCAATg                                      <  1:798692/150‑1 (MQ=255)                                                                                                         ggtTATTAATTTCAATGGCTGCCCATGTATTTGCACTTAGCAACAGCACAGCCAGAAGGGCTAAAACACGACTGAACATAGATACCTCCTCGACGGCTGACTTTGTGTGCTCTCCTCTGTGATGATCTTCTGATTTAATTTTAATCAATg                                      >  1:144558/1‑150 (MQ=255)                                                                                                         ggtTATTAATTTCAATGGCTGCCCATGTATTTGCACTTAGCAACAGCACAGCCAGAAGGGCTAAAACACGACTGAACATAGATACCTCCTCGACGGCTGACTTTGTGTGCTCTCCTCTGTGATGATCTTCTGATTTAATTTTAATCAATGa                                     <  1:125468/151‑1 (MQ=255)                                                                                                          gtTATTAATTTCAATGGCTGCCCATGTATTTGCACTTAGCAACAGCACAGCCAGAAGGGCTAAAACACGACTGAACATAGATACCTCCTCGACGGCTGACTTTGTGTGCTCTCCTCTGTGATGATCTTCTGATTTAATTTTAATCAATGAt                                    >  2:758425/1‑151 (MQ=255)                                                                                                             attaATTTCAATGGCTGCCCATGTATTTGCACTTAGCAACAGCACAGCCAGAAGGGCTAAAACACGACTGAACATAGATACCTCCTCGACGGCTGACTTTGTGTGCTCTCCTCTGTGATGATCTTCTGATTTAATTTTAATCAATGATaa                                  >  2:773851/1‑150 (MQ=255)                                                                                                                aaTTTCAATGGCTGCCCATGTATTTGCACTTAGCAACAGCACAGCCAGAAGGGCTAAAACACGACTGAACATAGATACCTCCTCGACGGCTGACTTTGTGTGCTCTCCTCTGTGATGATCTTCTGATTTAATTTTAATCAATGATAaaga                               <  1:168601/150‑1 (MQ=255)                                                                                                                 aTTTCAATGGCTGCCCATGTATTTGCACTTAGCAACAGCACAGCCAGAAGGGCTAAAACACGACTGAACATAGATACCTCCTCGACGGCTGACTTTGTGTGCTCTCCTCTGTGATGATCTTCTGATTTAATTTTAATCAATGATAaagaag                             <  2:431688/151‑1 (MQ=255)                                                                                                                   ttCAATGGCTGCCCATGTATTTGCACTTAGCAACAGCACAGCCAGAAGGGCTAAAACACGACTGAACATAGATACCTCCTCGACGGCTGACTTTGTGTGCTCTCCTCTGTGATGATCTTCTGATTTAATTTTAATCAATGATAAAGAAGtt                           >  1:511581/1‑151 (MQ=255)                                                                                                                       aTGGCTGCCCATGTATTTGCACTTAGCAACAGCACAGCCAGAAGGGCTAAAACACGACTGAACATAGATACCTCCTCGACGGCTGACTTTGTGTGCTCTCCTCTGTGATGATCTTCTGATTTAATTTTAATCAATGATAAAGAAGTTGATg                       <  1:647347/151‑1 (MQ=255)                                                                                                                       aTGGCTGCCCATGTATTTGCACTTAGCAACAGCACAGCCAGAAGGGCTAAAACACGACTGAACATAGATACCTCCTCGACGGCTGACTTTGTGTGCTCTCCTCTGTGATGATCTTCTGATTTAATTTTAATCAATGATAAAGAAGTTGATg                       >  2:708122/1‑151 (MQ=255)                                                                                                                            tGCCCATGTATTTGCACTTAGCAACAGCACAGCCAGAAGGGCTAAAACACGACTGAACATAGATACCTCCTCGACGGCTGACTTTGTGTGCTCTCCTCTGTGATGATCTTCTGATTTAATTTTAATCAATGATAAAGAAGTTGATGGTGAc                  >  2:740807/1‑151 (MQ=255)                                                                                                                                cATGTATTTGCACTTAGCAACAGCACAGCCAGAAGGGCTAAAACACGACTGAACATAGATACCTCCTCGACGGCTGACTTTGTGTGCTCTCCTCTGTGATGATCTTCTGATTTAATTTTAATCAATGATAAAGAAGTTGATGGTGACCAtt              <  1:758675/151‑1 (MQ=255)                                                                                                                                   gTATTTGCACTTAGCAACAGCACAGCCAGAAGGGCTAAAACACGACTGAACATAGATACCTCCTCGACGGCTGACTTTGTGTGCTCTCCTCTGTGATGATCTTCTGATTTAATTTTAATCAATGATAAAGAAGTTGATGGTGACCATTTCt           <  1:424961/151‑1 (MQ=255)                                                                                                                                     aTTTGCACTTAGCAACAGCACAGCCAGAAGGGCTAAAACACGACTGAACATAGATACCTCCTCGACGGCTGACTTTGTGTGCTCTCCTCTGTGATGATCTTCTGATTTAATTTTAATCAATGATAAAGAAGTTGATGGTGACCATTTCTg          <  2:876444/150‑1 (MQ=255)                                                                                                                                       ttGCACTTAGCAACAGCACAGCCAGAAGGGCTAAAACACGACTGAACATAGATACCTCCTCGACGGCTGACTTTGTGTGCTCTCCTCTGTGATGATCTTCTGATTTAATTTTAATCAATGATAAAGAAGTTGATGGTGACCATTTCTGATg       >  2:171032/1‑151 (MQ=255)                                                                                                                                         gCACTTAGCAACAGCACAGCCAGAAGGGCTAAAACACGACTGAACATAGATACCTCCTCGACGGCTGACTTTGTGTGCTCTCCTCTGTGATGATCTTCTGATTTAATTTTAATCAATGATAAAGAAGTTGATGGTGACCATTTCTGATGCa     <  2:229935/151‑1 (MQ=255)                                                                                                                                            cTTAGCAACAGCACAGCCAGAAGGGCTAAAACACGACTGAACATAGATACCTCCTCGACGGCTGACTTTGTGTGCTCTCCTCTGTGATGATCTTCTGATTTAATTTTAATCAATGATAAAGAAGTTGATGGTGACCATTTCTGATGCAgtt  >  2:668146/1‑151 (MQ=255)                                                                                                                                                    |                                                                                                                                               TGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGCCTAAGCTTTGCACATCGTCCATATTTCTGGCCTGGTGGTTATTAATTTCAATGGCTGCCCATGTATTTGCACTTAGCAAAAGCACAGCCAGAAGGGCTAAAACACGACTGAACATAGATACCTCCTCGACGGCTGACTTTGTGTGCTCTCCTCTGTGATGATCTTCTGATTTAATTTTAATCAATGATAAAGAAGTTGATGGTGACCATTTCTGATGCAGTT  >  NC_000913/4424609‑4424898

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑