Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	256,342	T→G	V122V (GTT→GTG)	gpt →	xanthine phosphoribosyltransferase; xanthine‑guanine phosphoribosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	256,342	0	T	G	100.0%	162.4 / NA	44	V122V (GTT→GTG)	gpt	xanthine phosphoribosyltransferase; xanthine‑guanine phosphoribosyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (22/22);  total (22/22)

GAAACGCGCAGAAGGCGATGGCGAAGGCTTCATCGTTATTGATGACCTGGTGGATACCGGTGGTACTGCGGTTGCGATTCGTGAAATGTATCCAAAAGCGCACTTTGTCACCATCTTCGCAAAACCGGCTGGTCGTCCGCTGGTTGATGACTATGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGG‑ATATGGGCGTCGTATT‑CGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGT  >  NC_000913/256198‑256488                                                                                                                                                 |                                                                                                                                                     gAAACGCGCAGAAGGCGATGGCGAAGGCTTCATCGTTATTGATGACCTGGTGGATACCGGTGGTACTGCGGTTGCGATTCGTGAAATGTATCCAAAAGCGCACTTTGTCACCATCTTCGCAAAACCGGCTGGTCGTCCGCTGGTggatg                                                                                                                                                  <  1:519786/149‑1 (MQ=255)    aCGCGCAGAAGGCGATGGCGAAGGCTTCATCGTTATTGATGACCTGGTGGATACCGGTGGTACTGCGGTTGCGATTCGTGAAATGTATCCAAAAGCGCACTTTGTCACCATCTTCGCAAAACCGGCTGGTCGTCCGCTGGTGGATGACTAt                                                                                                                                             <  2:281354/151‑1 (MQ=255)    aCGCGCAGAAGGCGATGGCGAAGGCTTCATCGTTATTGATGACCTGGTGGATACCGGTGGTACTGCGGTTGCGATTCGTGAAATGTATCCAAAAGCGCACTTTGTCACCATCTTCGCAAAACCGGCTGGTCGTCCGCTGGTGGATGACTAt                                                                                                                                             <  2:421372/151‑1 (MQ=255)                 gATGGCGAAGGCTTCATCGTTATTGATGACCTGGTGGATACCGGTGGTACTGCGGTTGCGATTCGTGAAATGTATCCAAAAGCGCACTTTGTCACCATCTTCGCAAAACCGGCTGGTCGTCCGCTGGTGGATGACTATGTTGTTGATATcc                                                                                                                                <  1:456302/151‑1 (MQ=255)                  aTGGCGAAGGCTTCATCGTTATTGATGACCTGGTGGATACCGGTGGTACTGCGGTTGCGATTCGTGAAATGTATCCAAAAGCGCACTTTGTCACCATCTTCGCAAAACCGGCTGGTCGTCCGCTGGTGGATGACTATGTTGTTGATATccc                                                                                                                               <  1:26518/151‑1 (MQ=255)                     gCGAAGGCTTCATCGTTATTGATGACCTGGTGGATACCGGTGGTACTGCGGTTGCGATTCGTGAAATGTATCCAAAAGCGCACTTTGTCACCATCTTCGCAAAACCGGCTGGTCGTCCGCTGGTGGATGACTATGTTGTTGATATCCCGCa                                                                                                                            <  2:712266/151‑1 (MQ=255)                                  cGTTATTGATGACCTGGTGGATACCGGTGGTACTGCGGTTGCGATTCGTGAAATGTATCCAAAAGCGCACTTTGTCACCATCTTCGCAAAACCGGCTGGTCGTCCGCTGGTGGATGACTATGTTGTTGATATCCCGCAAGATACCTGGAtt                                                                                                               <  1:85977/151‑1 (MQ=255)                                  cGTTATTGATGACCTGGTGGATACCGGTGGTACTGCGGTTGCGATTCGTGAAATGTATCCAAAAGCGCACTTTGTCACCATCTTCGCAAAACCGGCTGGTCGTCCGCTGGTGGATGACTATGTTGTTGATATCCCGCAAGATACCTGGAt                                                                                                                <  2:387998/150‑1 (MQ=255)                                    ttATTGATGACCTGGTGGATACCGGTGGTACTGCGGTTGCGATTCGTGAAATGTATCCAAAAGCGCACTTTGTCACCATCTTCGCAAAACCGGCTGGTCGTCCGCTGGTGGATGACTATGTTGTTGATATCCCGCAAGATACCTGGATTg                                                                                                              >  1:253732/1‑150 (MQ=255)                                    ttATTGATGACCTGGTGGATACCGGTGGTACTGCGGTTGCGATTCGTGAAATGTATCCAAAAGCGCACTTTGTCACCATCTTCGCAAAACCGGCTGGTCGTCCGCTGGTGGATGACTATGTTGTTGATATCCCGCAAGATACCTGGATTg                                                                                                              >  1:409731/1‑150 (MQ=255)                                    ttATTGATGACCTGGTGGATACCGGTGGTACTGCGGTTGCGATTCGTGAAATGTATCCAAAAGCGCACTTTGTCACCATCTTCGCAAAACCGGCTGGTCGTCCGCTGGTGGATGACTATGTTGTTGATATCCCGCAAGATACCTGGATTg                                                                                                              >  1:392142/1‑150 (MQ=255)                                     tATTGATGACCTGGTGGATACCGGTGGTACTGCGGTTGCGATTCGTGAAATGTATCCAAAAGCGCACTTTGTCACCATCTTCGCAAAACCGGCTGGTCGTCCGCTGGTGGATGACTATGTTGTTGATATCCCGCAAGATACCTGGATTGa                                                                                                             >  1:29911/1‑150 (MQ=255)                                         gatgaCCTGGTGGATACCGGTGGTACTGCGGTTGCGATTCGTGAAATGTATCCAAAAGCGCACTTTGTCACCATCTTCGCAAAACCGGCTGGTCGTCCGCTGGTGGATGACTATGTTGTTGATATCCCGCAAGATACCTGGATTGAACAg                                                                                                         <  2:228186/150‑1 (MQ=255)                                                 ggtggATACCAGTGGTACTGCAGTTGCGATTCGTGAAATGTATCCAAAAGCGCACTTTGTCTCCATCTTCGCAAAACCGGCTGGTCGTCCGCTGGTGGATGACTATGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGG‑a                                                                                                <  1:78987/150‑1 (MQ=255)                                                  gtggATACCGGTGGTACTGCGGTTGCGATTCGTGAAATGTATCCAAAAGCGCACTTTGTCACCATCTTCGCAAAACCGGCTGGTCGTCCGCTGGTGGATGACTATGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGG‑at                                                                                               >  2:280397/1‑150 (MQ=255)                                                            gtggtACTGCGGTTGCGATTCGTGAAATGTATCCAAAAGCGCACTTTGTCACCATCTTCGCAAAACCGGCTGGTCGTCCGCTGGTGGATGACTATGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGG‑ATATGGGcgtcg                                                                                     >  2:677258/1‑150 (MQ=255)                                                              ggtACTGCGGTTGCGATTCGTGAAATGTATCCAAAAGCGCACTTTGTCACCATCTTCGCAAAACCGGCTGGTCGTCCGCTGGTGGATGACTATGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGG‑ATATGGGCGTCGTAt                                                                                  >  1:49201/1‑151 (MQ=255)                                                                tACTGCGGTTGCGATTCGTGAAATGTATCCAAAAGCGCACTTTGTCACCATCTTCGCAAAACCGGCTGGTCGTCCGCTGGTGGATGACTATGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGG‑ATATGGGCGTCGTATT‑c                                                                               >  2:117883/1‑151 (MQ=255)                                                                 aCTGCGGTTGCGATTCGTGAAATGTATCCAAAAGCGCACTTTGTCACCATCTTCGCAAAACCGGCTGGTCGTCCGCTGGTGGATGACTATGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGG‑ATATGGGCGTCGTATT‑c                                                                               <  2:23785/150‑1 (MQ=255)                                                                 aCTGCGGTTGCGATTCGTGAAATGTATCCAAAAGCGCACTTTGTCACCATCTTCGCAAAACCGGCTGGTCGTCCGCTGGTGGATGACTATGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGG‑ATATGGGCGTCGTATT‑Cg                                                                              >  2:827061/1‑151 (MQ=255)                                                                   tGCGGTTGCGATTCGTGAAATGTATCCAAAAGCGCACTTTGTCACCATCTTCGCAAAACCGGCTGGTCGTCCGCTGGTGGATGACTATGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGG‑ATATGGGCGTCGTATT‑CGTc                                                                            >  1:447668/1‑151 (MQ=255)                                                                     cGGTTGCGATTCGTGAAATGTATCCAAAAGCGCACTTTGTCACCATCTTCGCAAAACCGGCTGGTCGTCCGCTGGTGGATGACTATGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGG‑ATATGGGCGTCGTATT‑CGTccc                                                                          >  2:799401/1‑151 (MQ=255)                                                                         tGCGATTCGTGAAATGTATCCAAAAGCGCACTTTGTCACCATCTTCGCAAAACCGGCTGGTCGTCCGCTGGTGGATGACTATGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGG‑ATATGGGCGTCGTATT‑CGTCCCGcc                                                                       >  1:524658/1‑150 (MQ=255)                                                                            gATTCGTGAAATGTATCCAAAAGCGCACTTTGTCACCATCTTCGCAAAACCGGCTGGTCGTCCGCTGGTGGATGACTATGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGG‑ATATGGGCGTCGTATT‑CGTCCCGCCAAtc                                                                   <  2:854489/151‑1 (MQ=255)                                                                                cGTGAAATGTATCCAAAAGCGCACTTTGTCACCATCTTCGCAAAACCGGCTGGTCGTCCGCTGGTGGATGACTATGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGG‑ATATGGGCGTCGTATT‑CGTCCCGCCAATCTcc                                                                <  1:648210/150‑1 (MQ=255)                                                                                   gAAATGTATCCAAAAGCGCACTTTGTCACCATCTTCGCAAAACCGGCTGGTCGTCCGCTGGTGGGTGACTATGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGG‑ATATGGGCGTCGTATT‑CGTCCCGCCAATCTCCGGTc                                                            >  2:504533/1‑151 (MQ=255)                                                                                        gTATCCAAAAGCGCACTTTGTCACCATCTTCGAAAAACCGGCTGGTCGTCCGCTGGTGGATGACTATGTTGTTGATATCCCGCAAGATCCCTGGATTGAACAGCCGTGGG‑ATATGGGCGTCGTATT‑CGTCCCGCCAATCTCCGGTCGCTa                                                        <  2:429259/150‑1 (MQ=255)                                                                                         tATCCAAAAGCGCACTTTGTCACCATCTTCGCAAAACCGGCTGGTCGTCCGCTGGTGGATGACTATGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGG‑ATATGGGCGTCGTATT‑CGTCCCGCCAATCTCCGGTCGCTAAt                                                      >  1:597896/1‑151 (MQ=255)                                                                                              aaaaCCGCACTTTGTCACCTTCTTCGAAAACCCGGCTGGTCGTCCGCTGGTGGATGACTATGTTGTTGATATCCCGCAAGATCCCTGGATTAAGCATCCGTGGG‑ATATGGGCGTCGTATT‑CGTCCCGCCAATCTCCGGTCGCTAATCttt                                                  <  1:90904/150‑1 (MQ=255)                                                                                                aaGCGCACTTTGTCCCCATCTTCGCAAAACCGGCTGGTCGTCCGCTGGTGGATGACTATGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGG‑ATATGGGCGTCGTATT‑CGTCCCGCCAATCTCCGGTCGCTAATCTTTTCa                                               <  2:203131/151‑1 (MQ=255)                                                                                                 aGCGCACTTTGTCACCATCTTCGCAAAACCGGCTGGTCGTCCGCTGGTGGATGACTATGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGG‑ATATGGGCGTCGTATT‑CGTCCCGCCAATCTCCGGTCGCTAATCTTTTCa                                               >  2:659828/1‑150 (MQ=255)                                                                                                    gcACTTTGTCACCATCTTCGCAAAACCGGCTGGTCGTCCGCTGGTGGATGACTATGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGG‑ATATGGGCGTCGTATT‑CGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGc                                           >  2:389828/1‑151 (MQ=255)                                                                                                         ttGTCCCCATCTTCGCAAAACCGGCTGGTCGTCCGCTGGTGGATGACTATGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGG‑ATATGGGCGTCGTATT‑CGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTg                                        <  1:235236/149‑1 (MQ=255)                                                                                                         ttGTCACCATCTTCGCAAAACCGGCTGGTCGTCCGCTGGTGGATGACTATGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGG‑ATATGGGCGTCGTATT‑CGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTgg                                       <  1:720642/150‑1 (MQ=255)                                                                                                         ttGTCACCATCTTCGCAAAACCGGCTGGTCGTCCGCTGGTGGATGACTATGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGG‑ATATGGGCGTCGTATT‑CGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGc                                      >  1:54088/1‑151 (MQ=255)                                                                                                              aCCATCTTCGCAAAACCGGCTGGTCGTCCGCTGGTGGATGACTATGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGG‑ATATGGGCGTCGTATT‑CGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGc                                 >  2:765754/1‑151 (MQ=255)                                                                                                              aCCATCTTCGCAAAACCGGCTGGTCGTCCGCTGGTGGATGACTATGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGG‑ATATGGGCGTCGTATT‑CGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGc                                 >  1:326960/1‑151 (MQ=255)                                                                                                               ccATCTTCGAAAACCCGGCTGGTCGTCCGCTGGTGGATGACTATGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGG‑ATATGGGCGTCGTATT‑CGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGc                                 <  2:224177/150‑1 (MQ=255)                                                                                                                     tCGCAAAACCGGCTGGTCGTCCGCTGGTGGATGACTATGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGG‑ATATGGGCGTCGTATT‑CGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCgg                              <  1:712462/147‑1 (MQ=255)                                                                                                                             cgggttGGTCGCCCGCGGGGGGATGCTTTTTTTGTGAATACCCCGAAAAAT‑CCTGGATTGAAACGCCGGGGGAATAGGGGCG‑CGTTTTCCTTCCGGCCAACCTCCGGTCGCTAACCTTTTCAACGCCTGGCACTGCCGGGCTTTGTtttt                   <  1:280389/145‑1 (MQ=255)                                                                                                                               ggCTGGTCGTCCGCTGGTGGATGACTATGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGG‑ATATGGGCGTCGTATT‑CGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCttttt                >  1:508304/1‑151 (MQ=255)                                                                                                                                  tGGTCGTCCGCTGGTGGATGACTATGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGG‑ATATGGGCGTCGTATT‑CGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAAc             >  2:691245/1‑151 (MQ=255)                                                                                                                                   ggTCGTCCGCTGGTGGATGACTATGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGG‑ATATGGGCGTCGTATT‑CGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACt            >  2:330484/1‑151 (MQ=255)                                                                                                                                       gtcCGCTGTTGGATGACTATGTTGTTGATATCCCGAAAGATCCCTGGATTAAACGGCCGTGGG‑ATATGGGCTTCGTATC‑CGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAg        <  2:267925/151‑1 (MQ=255)                                                                                                                                              ggTGGATGACTATGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGG‑ATATGGGCGTCGTATT‑CGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGt  <  2:200789/150‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                                     GAAACGCGCAGAAGGCGATGGCGAAGGCTTCATCGTTATTGATGACCTGGTGGATACCGGTGGTACTGCGGTTGCGATTCGTGAAATGTATCCAAAAGCGCACTTTGTCACCATCTTCGCAAAACCGGCTGGTCGTCCGCTGGTTGATGACTATGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGG‑ATATGGGCGTCGTATT‑CGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGT  >  NC_000913/256198‑256488

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑