Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,530,170	A→C	F25C (TTC→TGC)	sgcC ←	putative PTS system EIIC permease component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,530,170	0	A	C	100.0%	264.9 / NA	72	F25C (TTC→TGC)	sgcC	putative PTS system EIIC permease component
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (28/44);  total (28/44)

GACATGCAGGGCTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATACCGACAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCACGATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGACAGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTTAACAGAGCCTTGATTTGTTGTTTTAACGCGTCGTCGTTGATCCCTGTGA  >  NC_000913/4530022‑4530313                                                                                                                                                     |                                                                                                                                                gACATGCAGGGCTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATACCGACAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGCAg                                                                                                                                               <  2:313573/151‑1 (MQ=255)            cTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATACCGACAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGCAGATAAGGCCAAt                                                                                                                                    >  2:203431/1‑151 (MQ=255)             tAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATACCGACAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGCAGATAAGGCCAATc                                                                                                                                   >  2:784734/1‑151 (MQ=255)              aGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATACCGACAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGCAGATAAGGCCAATCa                                                                                                                                  <  2:929017/151‑1 (MQ=255)              aGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATACCGACAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGCAGATAAGGCCAATCa                                                                                                                                  <  1:540002/151‑1 (MQ=255)               ggCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATACCGACAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGCAGATAAGGCCAATCAc                                                                                                                                 >  1:327762/1‑151 (MQ=255)                gCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATACCGACAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGCAGATAAGGCCAATCACg                                                                                                                                <  1:206744/151‑1 (MQ=255)                    aaaCGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATACCGACAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGCAGATAAGGCCAATCACGATc                                                                                                                             >  1:768388/1‑150 (MQ=255)                    aaaCGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATACCGACAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGCAGATAAGGCCAATCACGATc                                                                                                                             >  1:235015/1‑150 (MQ=255)                    aaaCGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATACCGACAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGCAGATAAGGCCAATCACGATCa                                                                                                                            <  1:835330/151‑1 (MQ=255)                      aCGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATACCGACAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGCAGATAAGGCCAATCACGATCatg                                                                                                                          <  1:114197/151‑1 (MQ=255)                       cGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATACCGACAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGCAGATAAGGCCAATCACGATCatga                                                                                                                         <  2:300000/151‑1 (MQ=255)                       cGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATACCGACAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGCAGATAAGGCCAATCACGATCatg                                                                                                                          >  2:816087/1‑150 (MQ=255)                        gCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATACCGACAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGCAGATAAGGCCAATCACGATCatgat                                                                                                                        <  1:381839/151‑1 (MQ=255)                          tCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATACCGACAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGCAGATAAGGCCAATCACGATCatgatg                                                                                                                       >  1:675107/1‑150 (MQ=255)                          tCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATACCGACAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGCAGATAAGGCCAATCACGATCatgatg                                                                                                                       >  2:601765/1‑150 (MQ=255)                           cAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATACCGACAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGCAGATAAGGCCAATCACGATCatgatg                                                                                                                       <  2:133196/149‑1 (MQ=255)                               catcaCTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATACCGACAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGCAGATAAGGCCAATCACGATCATGATGATAgg                                                                                                                  <  2:831585/150‑1 (MQ=255)                                atcaCTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATACCGACAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGCAGATAAGGCCAATCACGATCATGATGATAGGGa                                                                                                                <  1:170255/151‑1 (MQ=255)                                    cTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATACCGACAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGCAGATAAGGCCAATCACGATCATGATGATAGGGACGa                                                                                                             >  1:583610/1‑150 (MQ=255)                                      ttGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATACCGACAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGCAGATAAGGCCAATCACGATCATGATGATAGGGACGaa                                                                                                            <  2:170496/149‑1 (MQ=255)                                        gATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATACCGACAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGCAGATAAGGCCAATCACGATCATGATGATAGGGACGAAAAc                                                                                                         <  1:13183/150‑1 (MQ=255)                                          tAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATACCGACAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGCAGATAAGGCCAATCACGATCATGATGATAGGGACGAAAACgg                                                                                                       >  1:664584/1‑150 (MQ=255)                                          tAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATACCGACAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGCAGATAAGGCCAATCACGATCATGATGATAGGGACGAAAACGGt                                                                                                      >  1:310499/1‑151 (MQ=255)                                            gggggACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATACCGACAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGCAGATAAGGCCAATCACGATCATGATGATAGGGACGAAAACGGTa                                                                                                     >  2:208952/1‑150 (MQ=255)                                                gACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATACCGACAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGCAGATAAGGCCAATCACGATCATGATGATAGGGACGAAAACGGTAccgcc                                                                                                <  1:591974/151‑1 (MQ=255)                                                    gAGGCTGTCGATCGCCATGACGATCACCAGTCCCATACCGACAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGCAGATAAGGCCAATCACGATCATGATGATAGGGACGAAAACGGTACCGCCga                                                                                              <  1:424454/149‑1 (MQ=255)                                                    gAGGCTGTCGATCGCCATGACGATCACCAGTCCCATACCGACAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGCAGATAAGGCCAATCACGATCATGATGATAGGGACGAAAACGGTACCGCCga                                                                                              <  1:67514/149‑1 (MQ=255)                                                          gTCGATCGCCATGACGATCACCAGTCCCATACCGACAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGCAGATAAGGCCAATCACGATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGACa                                                                                        <  2:42574/149‑1 (MQ=255)                                                           tCGATCGCCATGACGATCACCAGTCCCATACCGACAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGCAGATAAGGCCAATCACGATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGACAgg                                                                                      >  2:562021/1‑150 (MQ=255)                                                            cGATCGCCATGACGATCACCAGTCCCATACCGACAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGCAGATAAGGCCAATCACGATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGACAGGa                                                                                     >  1:364331/1‑150 (MQ=255)                                                             gATCGCCATGACGATCACCAGTCCCATACCGACAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGCAGATAAGGCCAATCACGATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGACAGGAt                                                                                    <  2:759697/150‑1 (MQ=255)                                                                 gCCATGACGATCACCAGTCCCATACCGACAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGCAGATAAGGCCAATCACGATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGACAGGATGTa                                                                                 <  1:117896/149‑1 (MQ=255)                                                                  ccATGACGATCACCAGTCCCATACCGACAAAACCAACCCCAACCGTTCCCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGCAGATAAGGCCAATCACGATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGACAGGATGTa                                                                                 <  1:928333/148‑1 (MQ=255)                                                                      gACGATCACCAGTCCCATACCGACAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGCAGATAAGGCCAATCACGATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGACAGGATGTAATCAAAc                                                                          <  2:535856/151‑1 (MQ=255)                                                                       aCGATCACCAGTCCCATACCGACAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGCAGATAAGGCCAATCACGATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGACAGGATGTAATCAAAc                                                                          >  2:338523/1‑150 (MQ=255)                                                                        cGATCACCAGTCCCATACCGACAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGCAGATAAGGCCAATCACGATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGACAGGATGTAATCAAACa                                                                         <  2:218655/150‑1 (MQ=255)                                                                        cGATCACCAGTCCCATACCGACAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGCAGATAAGGCCAATCACGATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGACAGGATGTAATCAAACAt                                                                        >  1:24777/1‑151 (MQ=255)                                                                          aTCACCAGTCCCATACCGACAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGCAGATAAGGCCAATCACGATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGACAGGATGTAATCAAACATa                                                                       >  1:2002/1‑150 (MQ=255)                                                                            cACCAGTCCTTTACCGACAAAACCAATCCCAACCTTTCCCCCTTCCTTAATCGCCTGCAGCCAGGAAATGCGCCAAATAAGGCCAATCACGATTAGGATGATAGGTACGAAAACGGAACCGCCGAGAGACAGGATGTAATCAAACa                                                                         <  1:185983/146‑1 (MQ=255)                                                                              ccAGTCCCATACCGACAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGCAGATAAGGCCAATCACGATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGACAGGATGTAATCAAACATAATg                                                                    <  2:768407/149‑1 (MQ=255)                                                                                aGTCCCATACCGACAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGCAGATAAGGCCAATCACGATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGACAGGATGTAATCAAACATAATGcctc                                                                <  1:398458/151‑1 (MQ=255)                                                                                     cATACCGACAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGCAGATAAGGCCAATCACGATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGACAGGATGTAATCAAACATAATGCCTCCtttt                                                           <  2:214616/151‑1 (MQ=255)                                                                                      aTACCGACAAAACCAACCCCAACCGTTCCCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGCAGATAAGGCCAATCACGATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGACAGGATGTAATCAAACATAATGCCTCCtttt                                                           <  1:898469/150‑1 (MQ=255)                                                                                         ccGACAAAACCAATCCCAACCGTTCCCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGCAGATAAGGCCAATCACGATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGACAGGATGTAATCAAACATAATGCCTCCTTTTAtt                                                        <  2:272710/150‑1 (MQ=255)                                                                                         ccGACAAAACCAACCCCAACCGTTCCCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGCAGATAAGGCCAATCACGATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGACAGGATGTAATCAAACATAATGCCTCCTTTTATTg                                                       <  1:816067/151‑1 (MQ=255)                                                                                            aCAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGCAGATAAGGCCAATCACGATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGACAGGATGTAATCAAACATAATGCCTCCTTTTATTGAGt                                                    <  2:846656/151‑1 (MQ=255)                                                                                              aaaaCCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGCAGATAAGGCCAATCACGATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGACAGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTTa                                                  <  2:53610/151‑1 (MQ=255)                                                                                                  ccAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGCAGATAAGGCCAATCACGATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGACAGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTTAAc                                                >  1:261127/1‑149 (MQ=255)                                                                                                    aaTCCCAACCGTTCCCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGCAGATAAGGCCAATCACGATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGACAGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTTAACaga                                             <  1:11585/150‑1 (MQ=255)                                                                                                    aaTCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGCAGATAAGGCCAATCACGATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGACAGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTTAACagag                                            <  2:162195/151‑1 (MQ=255)                                                                                                    aaTCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGCAGATAAGGCCAATCACGATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGACAGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTTAACag                                              >  1:829261/1‑149 (MQ=255)                                                                                                    aaTCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGCAGATAAGGCCAATCACGATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGACAGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTTAACag                                              >  1:403326/1‑149 (MQ=255)                                                                                                       cccAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGCAGATAAGGCCAATCACGATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGACAGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTTAACAGAGcc                                          <  2:276125/150‑1 (MQ=255)                                                                                                        ccAACCGTTACCCCTGCCTTAAGCGCCTGCAGCCAGGGGATGCGGCAGATAAGGCCAATCACGATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGACAGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTTAACAGAGCCtt                                        <  2:19500/151‑1 (MQ=255)                                                                                                            ccGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGCAGATAAGGCCAATCACGATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGACAGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTTAACAGAGCCTTGAt                                     <  1:386964/150‑1 (MQ=255)                                                                                                             cGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGCAGATAAGGCCAATCACGATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGACAGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTTAACAGAGCCTTGAttt                                   >  1:161246/1‑151 (MQ=255)                                                                                                                tACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGCAGATAAGGCCAATCACGATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGACAGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTTAACAGAGCCTTGATttgtt                                >  2:148014/1‑151 (MQ=255)                                                                                                                tACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGCAGATAAGGCCAATCACGATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGACAGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTTAACAGAGCCTTGATttgt                                 <  2:801801/150‑1 (MQ=255)                                                                                                                  ccccTGCTTTAATCGCCTGCAGCCGGGGGATGCGGCAGATAAGGCCAATCACGATCATTATGATAGGGACGAAAACGGTACCGCCGAGAGACAGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTTAACAGAGCCTTGATttgt                                 <  1:106098/148‑1 (MQ=255)                                                                                                                  ccccTGCCTTAATCGCCTGCAGCCAGGGGATGCGGCAGATAAGGCCAATCACGATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGACAGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTTAACAGAGCCTTGATTtgttgt                              >  1:48556/1‑151 (MQ=255)                                                                                                                   cccTGCCTTAATCGCCTGCAGCCAGGGGATGCGGCAGATAAGGCCAATCACGATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGACAGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTTAACAGAGCCTTGATTTgttgtt                             >  2:7470/1‑151 (MQ=255)                                                                                                                   cccTGCCTTAATCGCCTGCAGCCAGGGGATGCGGCAGATAAGGCCAATCACGATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGACAGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTTAACAGAGCCTTGATTTgttgtt                             <  2:777509/151‑1 (MQ=255)                                                                                                                         cTTAATCGCCTGCAGCCAGGGGATGCGGCAGATAAGGCCAATCACGATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGACAGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTTAACAGAGCCTTGCTTTGTTGTTTTAAc                        >  1:5889/1‑150 (MQ=255)                                                                                                                            aaTCGCCTGCAGCCAGGGGATGCGGCAGATAAGGCCAATCACGATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGACAGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTTAACAGAGCCTTGATTTGTTGTTTTAAcg                       <  2:771176/148‑1 (MQ=255)                                                                                                                                 ccTGCAGCCAGGGGATGCGGCAGATAAGGCCAATCACGATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGACAGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTTAACAGAGCCTTGATTTGTTGTTTTAACGcgtcgtcg               >  2:20921/1‑151 (MQ=255)                                                                                                                                      aGCCAGGGGATGCGGCAGATAAGGCCAATCACGATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGACAGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTTAACAGAGCCTTGATTTGTTGTTTTAACGCGTCGTCGTTGAt          >  2:854275/1‑151 (MQ=255)                                                                                                                                       gCCAGGGGATGCGGCAGATAAGGCCAATCACGATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGACAGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTTAACAGAGCCTTGATTTGTTGTTTTAACGCGTCGTCGTTGAt          <  2:151822/150‑1 (MQ=255)                                                                                                                                       gCCAGGGGATGCGGCAGATAAGGCCAATCACGATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGACAGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTTAACAGAGCCTTGATTTGTTGTTTTAACGCGTCGTCGTTGATc         >  2:76702/1‑151 (MQ=255)                                                                                                                                         cAGGGGAGGGGGCAGAAACGGCCAATCCCGATCATGAGGATAGGGAGCAAAGTAGTACCGCCGAGAGACAGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTTAACAGAGCCTTGATTTGTTGTTTTAACGCGTCGTCGTTg            <  1:201789/146‑1 (MQ=255)                                                                                                                                             ggATGCGGCAGATAAGGCCAATCACGATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGACAGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTTAACAGAGCCTTGATTTGTTGTTTTAACGCGTCGTCGTTGATCCCtgt    <  2:774855/150‑1 (MQ=255)                                                                                                                                              gATGCGGCAGATAAGGCCAATCACGATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGACAGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTTAACAGAGCCTTGATTTGTTGTTTTAACGCGTCGTCGTTGATCCCTGTGa  <  1:533495/151‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                GACATGCAGGGCTAGGCCGAAACGCTCAATCATCACTTTGATAGGGGGACTGAGGCTGTCGATCGCCATGACGATCACCAGTCCCATACCGACAAAACCAATCCCAACCGTTACCCCTGCCTTAATCGCCTGCAGCCAGGGGATGCGGAAGATAAGGCCAATCACGATCATGATGATAGGGACGAAAACGGTACCGCCGAGAGACAGGATGTAATCAAACATAATGCCTCCTTTTATTGAGTTAACAGAGCCTTGATTTGTTGTTTTAACGCGTCGTCGTTGATCCCTGTGA  >  NC_000913/4530022‑4530313

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑