Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,560,365	A→C	L106V (TTA→GTA)	yjiH ←	nucleoside recognition pore and gate family putative inner membrane transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,560,365	0	A	C	100.0%	187.2 / NA	52	L106V (TTA→GTA)	yjiH	nucleoside recognition pore and gate family putative inner membrane transporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (29/23);  total (29/23)

GTAATTTCACCTTCCTGCGCCAGCTCTTTGGTCATACCGGCGGCGGCATCGGTGTTTTGCAGGTTAGCGATCAGCGCCAGCGAGCAGATCCCTGGAATGCCCAACAACGGTTTTAGCACAGGTGTCATTAACTGCTGCGCGGCGCGTAATCCACCAAGACCATCGGTAATTGAAATAATCCCCAGAGAGAGAATGACTGACGGTGCCAGCTCCAGCGCAAAGAGAAAGCCATCTTTAGCCCCCGCGCCACCCGCGCCGCGAAATGAGGTGGTGGCTCCGTTCGCACCAGGCAGTTG  >  NC_000913/4560217‑4560512                                                                                                                                                     |                                                                                                                                                    gTAATTTCACCTTCCTGCGCCAGCTCTTTGGTCATACCGGCGGCGGCATCGGTGTTTTGCAGGTTAGCGATCAGCGCCAGCGAGCAGATCCCTGGAATGCCCAACAACGGTTTTAGCACAGGTGTCATTAACTGCTGCGCGGCGCGTACTc                                                                                                                                                   >  2:103063/1‑151 (MQ=255)   aaTTTCACCTTCCTGCGCCAGCTCTTTGGTCATACCGGCGGCGGCATCGGTGTTTTGCAGGTTAGCGATCAGCGCCAGCGAGCAGATCCCTGGAATGCCCAACAACGGTTTTAGCACAGGTGTCATTAACTGCTGCGCGGCGCGTACTcc                                                                                                                                                  >  2:657661/1‑150 (MQ=255)    aTTTCACCTTCCTGCGCCAGCTCTTTGGTCATACCGGCGGCGGCATCGGTGTTTTGCAGGTTAGCGATCAGCGCCAGCGAGCAGATCCCTGGAATGCCCAACAACGGTTTTAGCACAGGTGTCATTAACTGCTGCGCGGCGCGTACTccac                                                                                                                                                >  1:671107/1‑151 (MQ=255)       tCACCTTCCTGCGCCAGCTCTTTGGTCATACCGGCGGCGGCATCGGTGTTTTGCAGGTTAGCGATCAGCGCCAGCGAGCAGATCCCTGGAATGCCCAACAACGGTTTTAGCACAGGTGTCATTAACTGCTGCGCGGCGCGTACTccacca                                                                                                                                              >  2:693656/1‑150 (MQ=255)       tCACCTTCCTGCGCCAGCTCTTTGGTCATACCGGCGGCGGCATCGGTGTTTTGCAGGTTAGCGATCAGCGCCAGCGAGCAGATCCCTGGAATGCCCAACAACGGTTTTAGCACAGGTGTCATTAACTGCTGCGCGGCGCGTACTccacca                                                                                                                                              <  2:540885/150‑1 (MQ=255)          ccTTCCTGCGCCAGCTCTTTGGTCATACCGGCGGCGGCATCGGGGTTTTGCAGGTTAGCGATCAGCGCCAGCGAGCAGATCCCTGGAATGCCCAACAACGGATTTAGCACAGGTGTCATTAACTGCTGCGCGGCGCGTACTCCACCAAGa                                                                                                                                           >  2:478378/1‑150 (MQ=255)            ttCCTGCGCCAGCTCTTTGGTCATACCGGCGGCGGCATCGGTGTTTTGCAGGTTAGCGATCAGCGCCAGCGAGCAGATCCCTGGAATGCCCAACAACGGTTTTAGCACAGGTGTCATTAACTGCTGCGCGGCGCGTACTCCACCAAGAcc                                                                                                                                         >  2:655591/1‑150 (MQ=255)                       gCTCTTTGGTCATACCGGCGGCGGCATCGGTGTTTTGCAGGTTAGCGATCAGCGCCAGCGAGCAGATCCCTGGAATGCCCAACAACGGTTTTAGCACAGGTGTCATTAACTGCTGCGCGGCGCGTACTCCACCAAGACCATCGGTAATTGa                                                                                                                             <  1:2481/151‑1 (MQ=255)                           tttGGTCATACCGGCGGCGGCATCGGTGTTTTGCAGGTTAGCGATCAGCGCCAGCGAGCAGATCCCTGGAATGCCCAACAACGGTTTTAGCACAGGTGTCATTAACTGCTGCGCGGCGCGTACTCCACCAAGACCATCGGTAATTGAaat                                                                                                                          >  2:888306/1‑150 (MQ=255)                           tttGGTCATACCGGCGGCGGCATCGGTGTTTTGCAGGTTAGCGATCAGCGCCAGCGAGCAGATCCCTGGAATGCCCAACAACGGTTTTAGCACAGGTGTCATTAACTGCTGCGCGGCGCGTACTCCACCAAGACCATCGGTAATTGAaat                                                                                                                          >  1:327789/1‑150 (MQ=255)                              ggTCATACCGGCGGCGGCATCGGTGTTTTGCAGGTTAGCGATCAGCGCCAGCGAGCAGATCCCTGGAATGCCCAACAACGGTTTTAGCACAGGTGTCATTAACTGCTGCGCGGCGCGTACTCCACCAAGACCATCGGTAATTGAaataat                                                                                                                       <  2:301862/150‑1 (MQ=255)                                  aTACCGGCGGCGGCATCGGTGTTTTGCAGGTTAGCGATCAGCGCCAGCGAGCAGATCCCTGGAATGCCCAACAACGGTTTTAGCACAGGTGTCATTAACTGCTGCGCGGCGCGTACTCCACCAAGACCATCGGTAATTGAAATAATcccc                                                                                                                   >  2:23379/1‑150 (MQ=255)                                   tACCGGCGGCGGCATCGGTGTTTTGCAGGTTAGCGATCAGCGCCAGCGAGCAGATCCCTGGAATGCCCAACAACGGTTTTAGCACAGGTGTCATTAACTGCTGCGCGGCGCGTACTCCACCAAGACCATCGGTAATTGAAATAATcccc                                                                                                                   >  2:539749/1‑149 (MQ=255)                                                  cGGTGTTTTGCAGGTTAGCGATCAGCGCCAGCGAGCAGATCCCTGGAATGCCCAACAACGGTTTTAGCACAGGTGTCATTAACTGCTGCGCGGCGCGTACTCCACCAAGACCATCGGTAATTGAAATAATCCCCAGAGAGAGAAtgactga                                                                                                  <  1:175371/151‑1 (MQ=255)                                                          tGCAGGTTAGCGATCAGCGCCAGCGAGCAGATCCCTGGAATGCCCAACAACGGTTTTAGCACAGGTGTCATTAACTGCTGCGCGGCGCGTACTCCACCAAGACCATCGGTAATTGAAATAATCCCCAGAGAGAGAATGACTGACGGTGcc                                                                                           <  1:374116/150‑1 (MQ=255)                                                                ttAGCGATCAGCGCCAGCGAGCAGATCCCTGGAATGCCCAACAACGGTTTTAGCACAGGTGTCATTAACTGCTGCGCGGCGCGTACTCCACCAAGACCATCGGTAATTGAAATAATCCCCAGAGAGAGAATGACTGACGGTGCCAGCTcc                                                                                     >  1:347345/1‑150 (MQ=255)                                                                  aGCGATCAGCGCCAGCGAGCAGATCCCTGGAATGCCCAACAACGGTTTTAGCACAGGTGTCATTAACTGCTGCGCGGCGCGTACTCCACCAAGACCATCGGTAATTGAAATAATCCCCAGAGAGAGAATGACTGACGGTGCCAGCTCCAgc                                                                                  <  2:353474/151‑1 (MQ=255)                                                                  aGCGATCAGCGCCAGCGAGCAGATCCCTGGAATGCCCAACAACGGTTTTAGCACAGGTGTCATTAACTGCTGCGCGGCGCGTACTCCACCAAGACCATCGGTAATTGAAATAATCCCCAGAGAGAGAATGACTGACGGTGCCAGCTCCAgc                                                                                  <  2:95799/151‑1 (MQ=255)                                                                  aGCGATCAGCGCCAGCGAGCAGATCCCTGGAATGCCCAACAACGGTTTTAGCACAGGTGTCATTAACTGCTGCGCGGCGCGTACTCCACCAAGACCATCGGTAATTGAAATAATCCCCAGAGAGAGAATGACTGACGGTGCCAGCTCCAg                                                                                   <  1:565994/150‑1 (MQ=255)                                                                    cGATCAGCGCCAGCGAGCAGATCCCTGGAATGCCCAACAACGGTTTTAGCACAGGTGTCATTAACTGCTGCGCGGCGCGTACTCCACCAAGACCATCGGTAATTGAAATAATCCCCAGAGAGAGAATGACTGACGGTGCCAGCTCCAgc                                                                                  <  2:155137/149‑1 (MQ=255)                                                                        cAGCGCCAGCGAGCAGATCCCTGGAATGCCCAACAACGGTTTTAGCACAGGTGTCATTAACTGCTGCGCGGCGCGTACTCCACCAAGACCATCGGTAATTGAAATAATCCCCAGAGAGAGAATGACTGACGGTGCCAGCTCCAgcgc                                                                                >  1:494447/1‑147 (MQ=255)                                                                         aGCGCCAGCGAGCAGATCCCTGGAATGCCCAACAACGGTTTTAGCACAGGTGTCATTAACTGCTGCGCGGCGCGTACTCCACCAAGACCATCGGTAATTGAAATAATCCCCAGAGAGAGAATGACTGACGGTGCCAGCTCCAGCGCaa                                                                              >  2:363575/1‑148 (MQ=255)                                                                         aGCGCCAGCGAGCAGATCCCTGGAATGCCCAACAACGGTTTTAGCACAGGTGTCATTAACTGCTGCGCGGCGCGTACTCCACCAAGACCATCGGTAATTGAAATAATCCCCAGAGAGAGAATGACTGACGGTGCCAGCTCCAGCGCaa                                                                              >  1:677581/1‑148 (MQ=255)                                                                               aGCGAGCAGATCCCTGGAATGCCCAACAACGGTTTTAGCACAGGTGTCATTAACTGCTGCGCGGCGCGTACTCCACCAAGACCATCGGTAATTGAAATAATCCCCAGAGAGAGAATGACTGACGGTGCCAGCTCCAGCGCAAAGAGAAAGc                                                                     >  2:135207/1‑151 (MQ=255)                                                                                gCGAGCAGATCCCTGGAATGCCCAACAACGGTTTTAGCACAGGTGTCATTAACTGCTGCGCGGCGCGTACTCCACCAAGACCATCGGTAATTGAAATAATCCCCAGAGAGAGAATGACTGACGGTGCCAGCTCCAGCGCAAAGAGAAAGcc                                                                    >  1:734298/1‑151 (MQ=255)                                                                                gCGAGCAGATCCCTGGAATGCCCAACAACGGTTTTAGCACAGGTGTCATTAACTGCTGCGCGGCGCGTACTCCACCAAGACCATCGGTAATTGAAATAATCCCCAGAGAGAGAATGACTGACGGTGCCAGCTCCAGCGCAAAGAGAAAGcc                                                                    >  2:698873/1‑151 (MQ=255)                                                                                  gAGCAGATCCCTGGAATGCCCAACAACGGTTTTAGCACAGGTGTCATTAACTGCTGCGCGGCGCGTACTCCACCAAGACCATCGGTAATTGAAATAATCCCCAGAGAGAGAATGACTGACGGTGCCAGCTCCAGCGCAAAGAGAAAGCCAt                                                                  <  1:218709/151‑1 (MQ=255)                                                                                  gAGCAGATCCCTGGAATGCCCAACAACGGTTTTAGCACAGGTGTCATTAACTGCTGCGCGGCGCGTACTCCACCAAGACCATCGGTAATTGAAATAATCCCCAGAGAGAGAATGACTGACGGTGCCAGCTCCAGCGCAAAGAGAAAGCCAt                                                                  >  1:580110/1‑151 (MQ=255)                                                                                   aGCAGATCCCTGGAATGCCCAACAACGGTTTTAGCACAGGTGTCATTAACTGCTGCGCGGCGCGTACTCCACCAAGACCATCGGTAATTGAAATAATCCCCAGAGAGAGAATGACTGACGGTGCCAGCTCCAGCGCAAAGAGAAAGCCATc                                                                 <  1:598820/151‑1 (MQ=255)                                                                                   aGCAGATCCCTGGAATGCCCAACAACGGTTTTAGCACAGGTGTCATTAACTGCTGCGCGGCGCGTACTCCACCAAGACCATCGGTAATTGAAATAATCCCCAGAGAGAGAATGACTGACGGTGCCAGCTCCAGCGCAAAGAGAAAGCCATc                                                                 <  2:181280/151‑1 (MQ=255)                                                                                     cAGATCCCTGGAATGCCCAACAACGGTTTTAGCACAGGTGTCATTAACTGCTGCGCGGCGCGTACTCCACCAAGACCATCGGTAATTGAAATAATCCCCAGAGAGAGAATGACTGACGGTGCCAGCTCCAGCGCAAAGAGAAAGCCATCtt                                                               >  1:285573/1‑151 (MQ=255)                                                                                      aGATCCCTGGAATGCCCAACAACGGTTTTAGCACAGGGGTCATTAACTGCTGCGCGGCGCGTACTCCACCAAGACCATCGGTATTTGAAATAATCCCCAGAGAGAGAATGACTGAAGGTGCCAGCTCCAGCGCAAAGTGAAAGCCATCttt                                                              >  1:195111/1‑151 (MQ=255)                                                                                          cccTGGAATGCCCAACAACGGTTTTAGCACAGGTGTCATTAACTGCTGCGCGGCGCGTACTCCACCAAGACCATCGGTAATTGAAATAATCCCCAGAGAGAGAATGACTGACGGTGCCAGCTCCAGCGCAAAGAGAAAGCCATCTTTAGc                                                           <  1:721475/150‑1 (MQ=255)                                                                                             tGGAATGCCCAACAACGGTTTTAGCACAGGTGTCATTAACTGCTGCGCGGCGCGTACTCCACCAAGACCATCGGTAATTGAAATAATCCCCAGAGAGAGAATGACTGACGGTGCCAGCTCCAGCGCAAAGAGAAAGCCATCTTTAGccccc                                                       >  2:273669/1‑151 (MQ=255)                                                                                             tGGAATGCCCAACAACGGTTTTAGCACAGGTGTCATTAACTGCTGCGCGGCGCGTACTCCACCAAGACCATCGGTAATTGAAATAATCCCCAGAGAGAGAATGACTGACGGTGCCAGCTCCAGCGCAAAGAGAAAGCCATCTTTAGccccc                                                       >  2:495053/1‑151 (MQ=255)                                                                                                aaTGCCCAACAACGGTTTTAGCACAGGTGTCATTAACTGCTGCGCGGCGCGTACTCCACCAAGACCATCGGTAATTGAAATAATCCCCAGAGAGAGAATGACTGACGGTGCCAGCTCCAGCGCAAAGAGAAAGCCATCTTTAGCCCCcgcg                                                    <  1:93213/151‑1 (MQ=255)                                                                                                  tGCCCAACAACGGTTTTAGCACAGGTGTCATTAACTGCTGCGCGGCGCGTACTCCACCAAGACCATCGGTAATTGAAATAATCCCCAGAGAGAGAATGACTGACGGTGCCAGCTCCAGCGCAAAGAGAAAGCCATCTTTAGCCCCCGCGcc                                                  >  2:832261/1‑151 (MQ=255)                                                                                                           acGGTTTTAGCACAGGTGTCATTAACTGCTGCGCGGCGCGTACTCCACCAAGACCATCGGTAATTGAAATAATCCCCAGAGAGAGAATGACTGACGGTGCCAGCTCCAGCGCAAAGAGAAAGCCATCTTTAGCCCCCGCGCCACccgcgcc                                         <  2:571696/151‑1 (MQ=255)                                                                                                           acGGTTTTAGCACAGGTGTCATTAACTGCTGCGCGGCGCGTACTCCACCAAGACCATCGGTAATTGAAATAATCCCCAGAGAGAGAATGACTGACGGTGCCAGCTCCAGCGCAAAGAGAAAGCCATCTTTAGCCCCCGCGCCACccgcgcc                                         >  2:622758/1‑151 (MQ=255)                                                                                                                  tAGCACAGGTGTCATTAACTGCTGCGCGGCGCGTACTCCACCAAGACCATCGGTAATTGAAATAATCCCCAGAGAGAGAATGACTGACGGTGCCAGCTCCAGCGCAAAGAGAAAGCCATCTTTAGCCCCCGCGCCACCCGCGCCGCGAAAt                                  >  2:211850/1‑151 (MQ=255)                                                                                                                              cATTAACTGCTGCGCGGCGCGTACTCCACCAAGACCATCGGTAATTGAAATAATCCCCAGAGAGAGAATGACTGACGGTGCCAGCTCCAGCGCAAAGAGAAAGCCATCTTTAGCCCCCGCGCCACCCGCGCCGCGAAATGAGGTGGTGGc                       >  1:400564/1‑150 (MQ=255)                                                                                                                                  aaCTGCTGCGCGGCGCGTACTCCCCCAAGACCATCGGTAATTGAAATAATCCCCAGAGAGAGAATGACTGACGGTGCCAGCTCCAGCGCAAAGAGAAAGCCAGCTTTAGCCCCCGCGCCACCCGCGCCGCGAAATGAGGTGGTGTCTcc                    <  1:109530/149‑1 (MQ=255)                                                                                                                                  aaCTGCTGCGCGGCGCGTACTCCACCAAGACCATCGGTAATTGAAATAATCCCCAGAGAGAGAATGACTGACGGTGCCAGCTCCAGCGCAAAGAGAAAGCCATCTTTAGCCCCCGCGCCACCCGCGCCGCGAAATGAGGTGGTGGCTCCGt                  <  1:517514/151‑1 (MQ=255)                                                                                                                                   aCTGCTGCGCGGCGCGTACTCCACCAAGACCATCGGTAATTGAAATAATCCCCAGAGAGAGAATGACTGACGGTGCCAGCTCCAGCGCAAAGAGAAAGCCATCTTTAGCCCCCGCGCCACCCGCGCCGCGAAATGAGGTGGTGGCTCCGtt                 <  1:155110/151‑1 (MQ=255)                                                                                                                                     tgctgcGCGGCGCGTACTCCACCAAGACCATCGGTAATTGAAATAATCCCCAGAGAGAGAATGACTGACGGTGCCAGCTCCAGCGCAAAGAGAAAGCCATCTTTAGCCCCCGCGCCACCCGCGCCGCGAAATGAGGTGGTGGCTCCGTTCg               >  2:344386/1‑151 (MQ=255)                                                                                                                                          cgcggggcgTACTCCTCCAAGACCATCGGTAAGTGAAATAATCCCCAGAGAGAGAATGACTGACGGTGCCAGCTCCAGCGCAAAGAGAAAGCCATCTTTAGCCCCCGCGCCACCCGCGCCGCGAAATGAGGTGGTGGCTCCGTTCGCAcc           <  1:679976/150‑1 (MQ=255)                                                                                                                                             ggcgcgTACTCCACCAAGACCATCGGTAATTGAAATAATCCCCAGAGAGAGAATGACTGACGGTGCCAGCTCCAGCGCAAAGAGAAAGCCATCTTTAGCCCCCGCGCCACCCGCGCCGCGAAATGAGGTGGTGGCTCCGTTCGCACCAGGc       >  2:137346/1‑151 (MQ=255)                                                                                                                                             ggcgcgTACTCCACCAAGACCATCGGTAATTGAAATAATCCCCAGAGAGAGAATGACTGACGGTGCCAGCTCCAGCGCAAAGAGAAAGCCATCTTTAGCCCCCGCGCCACCCGCGCCGCGAAATGAGGTGGTGGCTCCGTTCGCACCAGGc       <  1:816422/151‑1 (MQ=255)                                                                                                                                                 cgTACTCCACCAAGACCATCGGTAATTGAAATAATCCCCAGAGAGAGAATGACTGACGGTGCCAGCTCCAGCGCAAAGAGAAAGCCATCTTTAGCCCCCGCGCCACCCGCGCCGCGAAATGAGGTGGTGGCTCCGTTCGCACCAGGCAGtt   <  2:883563/151‑1 (MQ=255)                                                                                                                                                 cgTACTCCACCAAGACCATCGGTAATTGAAATAATCCCCAGAGAGAGAATGACTGACGGTGCCAGCTCCAGCGCAAAGAGAAAGCCATCTTTAGCCCCCGCGCCACCCGCGCCGCGAAATGAGGTGGTGGCTCCGTTCGCACCAGGCAGtt   <  1:693636/151‑1 (MQ=255)                                                                                                                                                  gTACTCCACCAAGACCATCGGTAATTGAAATAATCCCCAGAGAGAGAATGACTGCCGGTGCCAGCTCCAGCGCAACGAGAAAGCCATCTTTAGCCCCCGCGCCCCCCGCGCCGCGAAATGAGGTGGTGGCTCCGTTCGCACCAGGCAGTTg  <  1:478363/151‑1 (MQ=255)                                                                                                                                                  gTACTCCACCAAGACCATCGGTAATTGAAATAATCCCCAGAGAGAGAATGACTGACGGTGCCAGCTCCAGCGCAAAGAGAAAGCCATCTTTAGCCCCCGCGCCACCCGCGCCGCGAAATGCGGTGGTGGCTCCGTTCGCACAAGGCAGTTg  >  1:756444/1‑151 (MQ=255)                                                                                                                                                     |                                                                                                                                                    GTAATTTCACCTTCCTGCGCCAGCTCTTTGGTCATACCGGCGGCGGCATCGGTGTTTTGCAGGTTAGCGATCAGCGCCAGCGAGCAGATCCCTGGAATGCCCAACAACGGTTTTAGCACAGGTGTCATTAACTGCTGCGCGGCGCGTAATCCACCAAGACCATCGGTAATTGAAATAATCCCCAGAGAGAGAATGACTGACGGTGCCAGCTCCAGCGCAAAGAGAAAGCCATCTTTAGCCCCCGCGCCACCCGCGCCGCGAAATGAGGTGGTGGCTCCGTTCGCACCAGGCAGTTG  >  NC_000913/4560217‑4560512

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑