Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,598,247	T→G	N399H (AAC→CAC)	opgB ←	OPG periplasmic biosynthetic phosphoglycerol transferases I (membrane‑bound) and II (soluble)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,598,247	0	T	G	100.0%	246.7 / NA	69	N399H (AAC→CAC)	opgB	OPG periplasmic biosynthetic phosphoglycerol transferases I (membrane‑bound) and II (soluble)
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (31/38);  total (31/38)

CGCCACCGAGAATGTCCAGCACCGTCGCGCCGTTATCCATCGTGTTACGCTTCACTGCCAGCGTCTCTTGCTGCGGCTTGTCGCCACGAATGACAAAAAACAGGTTATTGCGATCCTGCTTATTGAGGTATTTCCACGCCGTGTTGTTCATCGCTAAATGGTCAGAAGAGACGACGATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATGACTGATTCGGTTTACCGTCAAAATCATATTT  >  NC_000913/4598100‑4598394                                                                                                                                                    |                                                                                                                                                    cGCCACCGAGAATGTCCAGCACCGTCGCGCCGTTATCCATCGTGTTACGCTTCACTGCCAGCGTCTCTTGCTGCGGCTTGTCGCCACGAATGACAAAAAACAGGTTATTGCGATCCTGCTTATTGAGGTATTTCCACGCCGTGTTGTGCAt                                                                                                                                                  >  2:651988/1‑151 (MQ=255)       cGAGAATGTCCAGCACCGTCGCGCCGTTATCCATCGTGTTACGCTTCACTGCCAGCGTCTCTTGCTGCGGCTTGTCGCCACGAATGACAAAAAACAGGTTATTGCGATCCTGCTTATTGAGGTATTTCCACGCCGTGTTGTGCATCGCTa                                                                                                                                             >  1:609687/1‑150 (MQ=255)        gagaATGTCCAGCACCGTCGCGCCGTTATCCATCGTGTTACGCTTCACTGCCAGCGTCTCTTGCTGCGGCTTGTCGCCACGAATGACAAAAAACAGGTTATTGCGATCCTGCTTATTGAGGTATTTCCACGCCGTGTTGTGCATCGCTaaa                                                                                                                                           <  1:90489/151‑1 (MQ=255)             tGTCCAGCACCGTCGCGCCGTTATCCATCGTGTTACGCTTCACTGCCAGCGTCTCTTGCTGCGGCTTGTCGCCACGAATGACAAAAAACAGGTTATTGCGATCCTGCTTATTGAGGTATTTCCACGCCGTGTTGTGCATCGCTAAATGGTc                                                                                                                                      >  1:529627/1‑151 (MQ=255)              gccaaGCACCGTCGCGCCGTTACCCTTCGTGTTACGCTTCACTGCCAGCGTCTCTTGCTGCGGCTTGTCGCCACGAATGCCAAAAAACAGGTAATTGCGATCCTGCTTATTGAGGTATTTCCACGCCGTGTTGTGCATCGCTaaa                                                                                                                                           <  1:405630/141‑1 (MQ=255)                ccAGCACCGTCGCGCCGTTATCCATCGTGTTACGCTTCACTGCCAGCGTCTCTTGCTGCGGCTTGTCGCCACGAATGACAAAAAACAGGTTATTGCGATCCTGCTTATTGAGGTATTTCCACGCCGTGTTGTGCATCGCTAAATGGTc                                                                                                                                      >  2:912940/1‑148 (MQ=255)                   gCACCGTCGCGCCGTTATCCATCGTGTTACGCTTCACTGCCAGCGTCTCTTGCTGCGGCTTGTCGCCACGAATGACAAAAAACAGGTTATTGCGATCCTGCTTATTGAGGTATTTCCACGCCGTGTTGTGCATCGCTAAATGGTCaga                                                                                                                                   >  1:20390/1‑148 (MQ=255)                   gCACCGTCGCGCCGTTATCCATCGTGTTACGCTTCACTGCCAGCGTCTCTTGCTGCGGCTTGTCGCCACGAATGACAAAAAACAGGTTATTGCGATCCTGCTTATTGAGGTATTTCCACGCCGTGTTGTGCATCGCTAAATGGTCaga                                                                                                                                   >  2:162705/1‑148 (MQ=255)                   gCACCGTCGCGCCGTTATCCACCGTGTTACGCTTCACTGCCAGCGTCTCTTGCTGCGGCTTGTCGCCACGAATGACAAAAAACAGGTTATTGCGATCCTGCTTATTGAGGTATTTCCACGCCGTGTTGTGCATCGCTAAATGGTCagaaga                                                                                                                                <  1:265330/151‑1 (MQ=255)                    cACCGTCGCGCCGTTATCCATCGTGTTACGCTTCACTGCCAGCGTCTCTTGCTGCGGCTTGTCGCCACGAATGACAAAAAACAGGTTATTGCGATCCTGCTTATTGAGGTATTTCCACGCCGTGTTGTGCATCGCTAAATGGTCagaag                                                                                                                                 >  1:125924/1‑149 (MQ=255)                      ccGTCGCGCCGTTATCCATCGTGTTACGCTTCACTGCCAGCGTCTCTTGCTGCGGCTTGTCGCCACGAATGACAAAAAACAGGTTATTGCGATCCTGCTTATTGAGGTATTTCCACGCCGTGTTGTGCATCGCTAAATGGTCAGAAGAgac                                                                                                                             <  1:529997/151‑1 (MQ=255)                            cgcCGTTATCCATCGTGTTACGCTTCACTGCCAGCGTCTCTTGCTGCGGCTTGTCGCCACGAATGACAAAAAACAGGTTATTGCGATCCTGCTTATTGAGGTATTTCCACGCCGTGTTGTGCATCGCTAAATGGTCAGAAGAGACGACGAt                                                                                                                       <  1:356286/151‑1 (MQ=255)                                    tCCATCGTGTTACGCTTCACTGCCAGCGTCTCTTGCTGCGGCTTGTCGCCACGAATGACAAAAAACAGGTTATTGCGATCCTGCTTATTGAGGTATTTCCACGCCGTGTTGTGCATCGCTAAATGGTCAGAAGAGACGACGATGACGGTa                                                                                                                <  1:487326/150‑1 (MQ=255)                                      cATCGTGTTACGCTTCACTGCCAGCGTCTCTTGCTGCGGCTTGTCGCCACGAATGACAAAAAACAGGTTATTGCGATCCTGCTTATTGAGGTATTTCCACGCCGTGTTGTGCATCGCTAAATGGTCAGAAGAGACGACGATGACGGTATCt                                                                                                             >  2:270447/1‑151 (MQ=255)                                      cATCGTGTTACGCTTCACTGCCAGCGTCTCTTGCTGCGGCTTGTCGCCACGAATGACAAAAAACAGGTTATTGCGATCCTGCTTATTGAGGTATTTCCACGCCGTGTTGTGCATCGCTAAATGGTCAGAAGAGACGACGATGACGGTATCt                                                                                                             <  1:767197/151‑1 (MQ=255)                                       aTCGTGTTACGCTTCACTGCCAGCGTCTCTTGCTGCGGCTTGTCGCCACGAATGACAAAAAACAGGTTATTGCGATCCTGCTTATTGAGGTATTTCCACGCCGTGTTGTGCATCGCTAAATGGTCAGAAGAGACGACGATGACGGTATCtt                                                                                                            >  1:567028/1‑151 (MQ=255)                                         cGTGTTACGCTTCACTGCCAGCGTCTCTTGCTGCGGCTTGTCGCCACGAATGACAAAAAACAGGTTATTGCGATCCTGCTTATTGAGGTATTTCCACGCCGTGTTGTGCATCGCTAAATGGTCAGAAGAGACGACGATGACGGTATCttt                                                                                                           <  1:928320/150‑1 (MQ=255)                                              taagCTTCACTGCCAGCGTCTCTTGCTGCGGCTTGTCGCCCCGAATGACAAAAAACAGGTTATTGCGCTCCTGCTTATTGAGGTATTTCCACGCCGTGTTGTGCATCGCTAAATGGTCAGAAGAGACGACGATGACGGTATCTTTAAAc                                                                                                       >  1:320127/4‑149 (MQ=255)                                              tACGCTTCACTGCCAGCGTCTCTTGCTGCGGCTTGTCGCCACGAATGACAAAAAACAGGTTATTGCGATCCTGCTTATTGAGGTATTTCCACGCCGTGTTGTGCATCGCTAAATGGTCAGAAGAGACGACGATGACGGTATCTTTAAAcc                                                                                                      >  1:261972/1‑150 (MQ=255)                                               aCGCTTCACTGCCAGCGTCTCTTGCTGCGGCTTGTCGCCACGAATGACAAAAAACAGGTTATTGCGATCCTGCTTATTGAGGTATTTCCACGCCGTGTTGTGCATCGCTAAATGGTCAGAAGAGACGACGATGACGGTATCTTTAAACCAc                                                                                                    <  2:347407/151‑1 (MQ=255)                                                 gCTTCACTGCCAGCGTCTCTTGCTGCGGCTTGTCGCCACGAATGACAAAAAACAGGTTATTGCGATCCTGCTTATTGAGGTATTTCCACGCCGTGTTGTGCATCGCTAAATGGTCAGAAGAGACGACGATGACGGTATCTTTAAACCACgg                                                                                                  <  1:125012/151‑1 (MQ=255)                                                 gCTTCACTGCCAGCGTCTCTTGCTGCGGCTTGTCGCCACGAATGACAAAAAACAGGTTATTGCGATCCTGCTTATTGAGGTATTTCCACGCAGTGTTGTGCATCGCTAAATGGTCAGAAGAGACGACGATGACGGTATCTTTAAACCACgg                                                                                                  >  2:429118/1‑151 (MQ=255)                                                  cTTCACTGCCAGCGTCTCTTGCTGCGGCTTGTCGCCACGAATGACAAAAAACAGGTTATTGCGATCCTGCTTATTGAGGTATTTCCACGCCGTGTTGTGCATCGCTAAATGGTCAGAAGAGACGACGATGACGGTATCTTTAAACCACgg                                                                                                  >  1:767297/1‑150 (MQ=255)                                                   ttCACTGCCAGCGTCTCTTGCTGCGGCTTGTCGCCACGAATGACAAAAAACAGGTTATTGCGATCCTGCTTATTGAGGTATTTCCACGCCGTGTTGTGCATCGCTAAATGGTCAGAAGAGACGACGATGACGGTATCTTTAAACCACGGTg                                                                                                >  2:213989/1‑151 (MQ=255)                                                      aCTGCCAGCGTCTCTTGCTGCGGCTTGTCGCCACGAATGACAAAAAACAGGTTATTGCGATCCTGCTTATTGAGGTATTTCCACGCCGTGTTGTGCATCGCTAAATGGTCAGAAGAGACGACGATGACGGTATCTTTAAACCACGGTGACg                                                                                             >  1:79990/1‑151 (MQ=255)                                                       cTGCCAGCGTCTCTTGCTGCGGCTTGTCGCCACGAATGACAAAAAACAGGTTATTGCGATCCTGCTTATTGAGGTATTTCCACGCCGTGTTGTGCATCGCTAAATGGTCAGAAGAGACGACGATGACGGTATCTTTAACCCACgg                                                                                                  <  2:513578/145‑1 (MQ=255)                                                          ccAGCGTCTCTTGCTGCGGCTTGTCGCCACGAATGACAAAAAACAGGTTATTGCGATCCTGCTTATTGAGGTATTTCCACGCCGTGTTGTGCATCGCTAAATGGTCAGAAGAGACGACGATGACGGTATCTTTAAACCACGGTGACGCttt                                                                                         <  1:302093/151‑1 (MQ=255)                                                             gCGTCTCTTGCTGCGGCTTGTCGCCACGAATGACAAAAAACAGGTTATTGCGATCCTGCTTATTGAGGTATTTCCACGCCGTGTTGTGCATCGCTAAATGGTCAGAAGAGACGACGATGACGGTATCTTTAAACCACGGTGACGCTTTGAt                                                                                      >  2:668240/1‑151 (MQ=255)                                                                tctcTTGCTGCGGCTTGTCGCCACGAATGACAAAAAACAGGTTATTGCGATCCTGCTTATTGAGGTATTTCCACGCCGTGTTGTGCATCGCTAAATGGTCAGAAGAGACGACGATGACGGTATCTTTAAACCACGGTGACGCTTTGAtttt                                                                                   >  1:888737/1‑151 (MQ=255)                                                                         gcGGCTTGTCGCCACGAATGACAAAAAACAGGTTATTGCGATCCTGCTTATTGAGGTATTTCCACGCCGTGTTGTGCATCGCTAAATGGTCAGAAGAGACGACGATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATa                                                                            <  1:497500/149‑1 (MQ=255)                                                                             cTTGTCGCCACGAATGACAAAAAACAGGTTATTGCGATCCTGCTTATTGAGGTATTTCCACGCCGTGTTGTGCATCGCTAAATGGTCAGAAGAGACGACGATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTc                                                                      >  1:763336/1‑151 (MQ=255)                                                                              ttGTCGCCACGAATGACAAAAAACAGGTTATTGCGATCCTGCTTATTGAGGTATTTCCACGCCGTGTTGTGCATCGCTAAATGGTCAGAAGAGACGACGATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTcg                                                                     >  1:527157/1‑151 (MQ=255)                                                                               tGTCGCCACGAATGACAAAAAACAGGTTATTGCGATCCTGCTTATTGAGGTATTTCCACGCCGTGTTGTGCATCGCTAAATGGTCAGAAGAGACGACGATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTcgc                                                                    >  1:351233/1‑151 (MQ=255)                                                                               tGTCGCCACGAATGACAAAAAACAGGTTATTGCGATCCTGCTTATTGAGGTATTTCCACGCCGTGTTGTGCATCGCTAAATGGTCAGAAGAGACGACGATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTcgc                                                                    >  2:86363/1‑151 (MQ=255)                                                                               tGTCGCCACGAATGACAAAAAACAGGTTATTGCGATCCTGCTTATTGAGGTATTTCCACGCCGTGTTGTGCATCGCTAAATGGTCAGAAGAGACGACGATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTc                                                                      <  1:735371/149‑1 (MQ=255)                                                                                  cGCCACGAATGACAAAAAACAGGTTATTGCGATCCTGCTTATTGAGGTATTTCCACGCCGTGTTGTGCATCGCTAAATGGTCAGAAGAGACGACGATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGAt                                                                 >  1:61089/1‑151 (MQ=255)                                                                                       cGAATGACAAAAAACAGGTTATTGCGATCCTGCTTATTGAGGTATTTCCACGCCGTGTTGTGCATCGCTAAATGGTCAGAAGAGACGACGATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTtct                                                            <  1:891725/151‑1 (MQ=255)                                                                                            gACAAAAAACAGGTTATTGCGATCCTGCTTATTGAGGTATTTCCACGCCGTGTTGTGCATCGCTAAATGGTCAGAAGAGACGACGATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTg                                                        <  2:840110/150‑1 (MQ=255)                                                                                             aCAAAAAACAGGTTATTGCGATCCTGCTTATTGAGGTATTTCCACGCCGTGTTGTGCATCGCTAAATGGTCAGAAGAGACGACGATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGc                                                      <  1:356584/151‑1 (MQ=255)                                                                                              cAAAAAACAGGTTATTGCGATCCTGCTTATTGAGGTATTTCCACGCCGTGTTGTGCATCGCTAAATGGTCAGAAGAGACGACGATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCt                                                     <  1:58039/151‑1 (MQ=255)                                                                                               aaaaaaCAGGTTATTGCGATCCTGCTTATTGAGGTATTTCCACGCCGTGTTGTGCATCGCTAAATGGTCAGAAGAGACGACGATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTg                                                    >  2:596429/1‑151 (MQ=255)                                                                                                  aaaCAGGTTATTGCGATCCTGCTTATTGAGGTATTTCCACGCCGTGTTGTGCATCGCTAAATGGTCAGAAGAGACGACGATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCaa                                                 <  2:908373/151‑1 (MQ=255)                                                                                                         ttATTGCGATCCTGCTTATTGAGGTATTTCCACGCCGTGTTGTGCATCGCTAAATGGTCAGAAGAGACGACGATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACcg                                          >  1:712429/1‑151 (MQ=255)                                                                                                          tATTGCGATCCTGCTTATTGAGGTATTTCCACGCCGTGTTGTGCATCGCTAAATGGTCAGAAGAGACGACGATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACcgc                                         >  2:611934/1‑151 (MQ=255)                                                                                                          tATTGCGATCCTGCTTATTGAGGTATTTCCACGCCGTGTTGTGCATCGCTAAATGGTCAGAAGAGACGACGATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACcg                                          <  2:675264/150‑1 (MQ=255)                                                                                                           aTTGCGATCCTGCTTATTGAGGTATTTCCACGCCGTGTTGTGCATCGCTAAATGGTCAGAAGAGACGACGATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACcgc                                         <  2:61834/150‑1 (MQ=255)                                                                                                              gCGATCCTGCTTATTGAGGTATTTCCACGCCGTGTTGTGCATCGCTAAATGGTCAGAAGAGACGACGATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCt                                      <  1:368525/150‑1 (MQ=255)                                                                                                              gCGATCCTGCTTATTGAGGTATTTCCACGCCGTGTTGTGCATCGCTAAATGGTCAGAAGAGACGACGATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCt                                      <  2:91278/150‑1 (MQ=255)                                                                                                              gCGATCCTGCTTATTGAGGTATTTCCACGCCGTGTTGTGCATCACTAAATGGTCAGAAGAGACGACGATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTg                                     <  1:289080/151‑1 (MQ=255)                                                                                                                gATCCTGCTTATTGAGGTATTTCCACGCCGTGTTGTGCATCGCTAAATGGTCAGAAGAGACGACGATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGa                                    >  1:687163/1‑150 (MQ=255)                                                                                                                   ccTGCTTATTGAGGTATTTCCACGCCGTGTTGTGCATCGCTAAATGGTCAGAAGAGACGACGATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATg                                 >  1:328754/1‑150 (MQ=255)                                                                                                                         tATTGAGGTATTTCCACGCCGTGTTGTGCATCGCTAAATGGTCAGAAGAGACGACGATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATGACTGa                            <  2:884683/149‑1 (MQ=255)                                                                                                                          aTTGAGGTATTTCCACGCCGTGTTGTGCATCGCTAAATGGTCAGAAGAGACGACGATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATGACTGATTc                         <  2:356708/151‑1 (MQ=255)                                                                                                                            tGAGGTATTTCCACGCCGTGTTGTGCATCGCTAAATGGTCAGAAGAGACGACGATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATGACTGATTCg                        <  2:179157/150‑1 (MQ=255)                                                                                                                             gAGGTATTTCCACGCCGTGTTGTGCATCGCTAAATGGTCAGAAGAGACGACGATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATGACTGATTCGGt                      <  2:696628/151‑1 (MQ=255)                                                                                                                             gAGGTATTTCCACGCCGTGTTGTGCATCGCTAAATGGTCAGAAGAGACGACGATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATGACTGATTCGGt                      >  2:171340/1‑151 (MQ=255)                                                                                                                             gAGGTATTTCCACGCCGTGTTGTGCATCGCTAAATGGTCAGAAGAGACGACGATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATGACTGATTCGGt                      <  1:213576/151‑1 (MQ=255)                                                                                                                                  aTTTCCACGCCGTGTTGTGCATCGCTAAATGGTCAGAAGAGACGACGATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATGACTGATTCGGTTTAcc                 <  2:317167/151‑1 (MQ=255)                                                                                                                                    ttCCACGCCGTGTTGTGCATCGCTAAATGGTCAGAAGAGACGACGATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATGACTGATTCGGTTTACCg                <  1:859456/150‑1 (MQ=255)                                                                                                                                    ttCCACGCCGTGTTGTGCATCGCTAAATGGTCAGAAGAGACGACGATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATGACTGATTCGGTTTACCg                <  1:26545/150‑1 (MQ=255)                                                                                                                                     tCCACGCCGTGTTGTGCATCGCTAAATGGTCAGAAGAGACGACGATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATGACTGATTCGGTTTACCGt               <  1:324270/150‑1 (MQ=255)                                                                                                                                      ccACGCCGTGTTGTGCATCGCTAAATGGTCAGAAGAGACGACGATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATGACTGATTCGGTTTACCGTCa             <  1:415345/151‑1 (MQ=255)                                                                                                                                       cACGCCGTGTTGTGCATCGCTAAATGGTCAGAAGAGACGACGATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATGACTGATTCGGTTTACCGTCaa            <  1:44337/151‑1 (MQ=255)                                                                                                                                         cGCCGTGTTGTGCATCGCTAAATGGTCAGAAGAGACGACGATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATGACTGATTCGGTTTACCGTCaaaa          <  2:726244/151‑1 (MQ=255)                                                                                                                                         cGCCGTGTTGTGCATCGCTAAATGGTCAGAAGAGACGACGATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATGACTGATTCGGTTTACCGTCaaaa          <  1:266098/151‑1 (MQ=255)                                                                                                                                          gCCGTGTTGTGCATCGCTAAATGGTCAGAAGAGACGACGATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATGACTGATTCGGTTTACCGTCAAAAt         >  1:178270/1‑151 (MQ=255)                                                                                                                                          gCCGTGTTGTGCATCGCTAAATGGTCAGAAGAGACGACGATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATGACTGATTCGGTTTACCGTCAAAAt         >  1:340432/1‑151 (MQ=255)                                                                                                                                          gCCGTGTTGTGCATCGCTAAATGGTCAGAAGAGACGACGATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATGACTGATTCGGTTTACCGTCAAAAt         >  1:344251/1‑151 (MQ=255)                                                                                                                                          gCCGTGTTGTGCATCGCTAAATGGTCAGAAGAGACGACGATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATGACTGATTCGGTTTACCGTCAAAAt         <  1:790563/151‑1 (MQ=255)                                                                                                                                                 tgtgCATCGCTAAATGGTCAGAAGAGACGACGATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATGACTGATTCGGTTTACCGTCAAAATCATAttt  >  1:487959/1‑151 (MQ=255)                                                                                                                                                    |                                                                                                                                                    CGCCACCGAGAATGTCCAGCACCGTCGCGCCGTTATCCATCGTGTTACGCTTCACTGCCAGCGTCTCTTGCTGCGGCTTGTCGCCACGAATGACAAAAAACAGGTTATTGCGATCCTGCTTATTGAGGTATTTCCACGCCGTGTTGTTCATCGCTAAATGGTCAGAAGAGACGACGATGACGGTATCTTTAAACCACGGTGACGCTTTGATTTTGTTGATAAACGTCGCGATGTTCTCCTGGCTGCAACTTACCGCGCTGAATGACTGATTCGGTTTACCGTCAAAATCATATTT  >  NC_000913/4598100‑4598394

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑