Predicted mutation | ||||||
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evidence | seq id | position | mutation | annotation | gene | description |
RA | NC_000913 | 266,989 | A→C | intergenic (‑22/+195) | ykfK ← / ← yafZ | CP4‑6 prophage; putative DNA‑binding transcriptional regulator;Phage or Prophage Related/CP4‑6 prophage; conserved protein |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 266,989 | 0 | A | C | 98.3% | 216.1 / NA | 58 | intergenic (‑22/+195) | ykfK/yafZ | CP4‑6 prophage; putative DNA‑binding transcriptional regulator;Phage or Prophage Related/CP4‑6 prophage; conserved protein |
Reads supporting (aligned to +/- strand): ref base A (0/1); new base C (24/33); total (24/34) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 |
CGCACCGACACACCAAACTCAGCGGCAAGCTTACGCACACTCAGCGTTTCCCCTGCCACCAGACGGCTGATTATCAGTGACAGCCTGACAGCCAGCCGGTCATGGCGGCGTTCTGCCTGTGTCATGAGACGTTCTCCGTGAAAGTTAACTGACTGAAAATGATGTGATTACTTTAAAGAGGGGGTCGGACAGGGTATGGACACCACAGAAACTATTTTTCATTTCTGCAAAAGCCAGAGGCAGCGGGGGTTACAGGCCTTCCCTCGGTGAGTGAGGACCGTCAGCACGGTG > NC_000913/266843‑267133 | cgcACCGACACACCAAACTCAGCGGCAAGCTTACGCACACTCAGCGTTTCCCCTGCCACCAGACGGCTGATTATCAGTGACAGCCTGACAGCCAGCCGGTCATGGCGGCGTTCTGCCTGTGTCATGAGACGTTCTCCGTGAAAGTTCactg > 2:295253/1‑151 (MQ=255) ccAAACTCAGCGGCAAGCTTACGCACACTCAGCGTTTCCCCTGCCACCAGACGGCTGATTATCAGTGACAGCCTGACAGCCAGCCGGTCATGGCGGCGTTCTGCCTGTGTCATGAGACGTTCTCCGTGAAAGTTCACTGACTGAAAatgat < 1:403434/151‑1 (MQ=255) cAAACTCAGCGGCAAGCTTACGCACACTCAGCGTTTCCCCTGCCACCAGACGGCTGATTATCAGTGACAGCCTGACAGCCAGCCGGTCATGGCGGCGTTCTGCCTGTGTCATGAGACGTTCTCCGTGAAAGTTCACTGACTGAAAatgatg < 2:329677/151‑1 (MQ=255) aaaCTCAGCGGCAAGCTTACGCACACTCAGCGTTTCCCCTGCCACCAGACGGCTGATTATCAGTGACAGCCTGACAGCCAGCCGGTCATGGCGGCGTTCTGCCTGTGTCATGAGACGCTCTCCGTGAAAGTTCACTGACTGAAAATGAtgt < 1:769247/151‑1 (MQ=255) aaCTCAGCGGCAAGCTTACGCACACTCAGCGTTTCCCCTGCCACCAGACGGCTGATTATCAGTGACAGCCTGACAGCCAGCCGGTCATGGCGGCGTTCTGCCTGTGTCATGAGACGTTCTCCGTGAAAGTTCACTGACTGAAAATGAtgtg > 2:863723/1‑151 (MQ=255) tCAGCGGCAAGCTTACGCACACTCAGCGTTTCCCCTGCCACCAGACGGCTGATTATCAGTGACAGCCTGACAGCCAGCCGGTCATGGCGGCGTTCTGCCTGTGTCATGAGACGTTCTCCGTGAAAGTTCACTGACTGAAAATGATGTGAtt > 1:743736/1‑151 (MQ=255) cAGCGGCAAGCTTACGCACACTCAGCGTTTCCCCTGCCACCAGACGGCTGATTATCAGTGACAGCCTGACAGCCAGCCGGTCATGGCGGCGTTCTGCCTGTGTCATGAGACGTTCTCCGTGAAAGTTCACTGACTGAAAATGATGTGAtt < 2:783362/150‑1 (MQ=255) ggCAAGCTTACGCACACTCAGCGTTTCCCCTGCCACCAGACGGCTGATTATCAGTGACAGCCTGACAGCCAGCCGGTCATGGCGGCGTTCTGCCTGTGTCATGAGACGTTCTCCGTGAAAGTTCACTGACTGAAAATGATGTGATTACttt > 2:410277/1‑151 (MQ=255) ggCAAGCTTACGCACACTCAGCGTTTCCCCTGCCACCAGACGGCTGATTATCAGTGACAGCCTGACAGCCAGCCGGTCATGGCGGCGTTCTGCCTGTGTCATGAGACGTTCTCCGTGAAAGTTCACTGACTGAAAATGATGTGATTACttt < 1:248972/151‑1 (MQ=255) ggCAAGCTTACGCACACTCAGCGTTTCCCCTGCCACCAGACGGCTGATTATCAGTGACAGCCTGACAGCCAGCCGGTCATGGCGGCGTTCTGCCTGTGTCATGAGACGTTCTCCGTGAAAGTTCACTGACTGAAAATGATGTGATTACttt > 2:165544/1‑151 (MQ=255) gCAAGCTTACGCACACTCAGCGTTTCCCCTGCCACCAGACGGCTGATTATCAGTGACAGCCTGACAGCCAGCCGGTCATGGCGGCGTTCTGCCTGTGTCATGAGACGTTCTCCGTGAAAGTTCACTGACTGAAAATGATGTGATTACTTTa < 2:674246/151‑1 (MQ=255) cAAGCTTACGCACACTCAGCGTTTCCCCTGCCACCAGACGGCTGATTATCAGTGACAGCCTGACAGCCAGCCGGTCATGGCGGCGTTCTGCCTGTGTCATGAGACGTTCTCCGTGAAAGTTCACTGACTGAAAATGATGTGATTACTTTaa < 2:651068/151‑1 (MQ=255) cacaCTCAGCGTTTCCCCTGCCCCCAGACGGCTGATTATCAGTGACAGCCTGACAGCCAGCCGGTCATGGCGGCGTTCTGCCTGTGTCATGAGACGTTCTCCGTGAAAGTTCACTGACTGAAAATGATGTGATTACTTTAAAGAGGGGGTc < 2:444841/151‑1 (MQ=255) cacTCAGCGTTTCCCCTGCCACCAGACGGCTGATTATCAGTGACAGCCTGACAGCAAGCCGGTCATGGCGGCGTTCTGCCTGTGTCATGAGACGTTCTCCGTGAAAGTTCACTGACTGAAAATGATGTGATTACTTTAAAGAGGGGGTCgg > 1:749719/1‑151 (MQ=255) gCGTTTCCCCTGCCACCAGACGGCTGATTATCAGTGACAGCCTGACAGCCAGCCGGTCATGGCGGCGTTCTGCCTGTGTCATGAGACGTTCTCCGTGAAAGTTCACTGACTGAAAATGATGTGATTACTTTAAAGAGGGGGTCGGACAgg < 1:819572/150‑1 (MQ=255) cGTTTCCCCTGCCACCAGACGGCTGATTATCAGTGACAGCCTGACAGCCAGCCGGTCATGGCGGCGTTCTGCCTGTGTCATGAGACGTTCTCCGTGAAAGTTCACTGACTGAAAATGATGTGATTACTTTAAAGAGGGGGTCGGACAggg > 1:781276/1‑150 (MQ=255) tttCCCCTGCCACCAGACGGCTGATTATCAGTGACAGCCTGACAGCCAGCCGGTCATGGCGGCGTTCTGCCTGTGTCATGAGACGTTCTCCGTGAAAGTTCACTGACTGAAAATGATGTGATTACTTTAAAGAGGGGGTCGGACAGGGTa > 1:497281/1‑150 (MQ=255) tCCCCTGCCACCAGACGGCTGATTATCAGTGACAGCCTGACAGCCAGCCGGTCATGGCGGCGTTCTGCCTGTGTCATGAGACGTTCTCCGTGAAAGTTCACTGACTGAAAATGATGTGATTACTTTAAAGAGGGGGTCGGACAGGGTATgg > 2:216635/1‑151 (MQ=255) tGCCACCAGACGGCTGATTATCAGTGACAGCCTGACAGCCAGCCGGTCATGGCGGCGTTCTGCCTGTGTCATGAGACGTTCTCCGTGAAAGTTCACTGACTGAAAATGATGTGATTACTTTAAAGAGGGGGTCGGACAGGGTATGGAcacc > 2:642294/1‑151 (MQ=255) gCCACCAGACGGCTGATTATCAGTGACAGCCTGACAGCCAGCCGGTCATGGCGGCGTTCTGCCTGTGTCATGAGACGTTCTCCGTGAAAGTTCACTGACTGAAAATGATGTGATTACTTTAAAGAGGGGGTCGGACAGGGTATGGacac < 2:809200/149‑1 (MQ=255) gCCACCAGACGGCTGATTATCAGTGACAGCCTGACAGCCAGCCGGTCATGGCGGCGTTCTGCCTGTGTCATGAGACGTTCTCCGTGAAAGTTCACTGACTGAAAATGATGTGATTACTTTAAAGAGGGGGTCGGACAGGGTATGGAcacc < 1:272717/150‑1 (MQ=255) ccaccaGACGGCTGATTATCAGTGACAGCCTGACAGCCAGCCGGTCATGGCGGCGTTCTGCCTGTGTCATGAGACGTTCTCCGTGAAAGTTCACTGACTGAAAATGATGTGATTACTTTAAAGAGGGGGTCGGACAGGGTATGGAcaccac < 2:752411/151‑1 (MQ=255) ccaGACGGCTGATTATCAGTGACAGCCTGACAGCCAGCCGGTCATGGCGGCGTTCTGCCTGTGTCATGAGACGTTCTCCGTGAAAGTTCACTGACTGAAAATGATGTGATTACTTTAAAGAGGGGGTCGGACAGGGTATGGAcaccac > 2:607798/1‑148 (MQ=255) caGACGGCTGATTATCAGTGACAGCCTGACAGCCAGCCGGTCATGGCGGCGTTCTGCCTGTGTCATGAGACGTTCTCCGTGAAAGTTCACTGACTGAAAATGATGTGATTACTTTAAAGAGGGGGTCGGACAGGGTATGGACACCACAGaa < 2:191416/151‑1 (MQ=255) aCGGCTGATTATCAGTGACAGCCTGACAGCCAGCCGGTCATGGCGGCGTTCTGCCTGTGTCATGAGACGTTCTCCGTGAAAGTTCACTGACTGAAAATGATGTGATTACTTTAAAGAGGGGGTCGGACAGGGTATGGACACCACAGAAACt < 2:866551/151‑1 (MQ=255) aCGGCTGATCATCAGTGACAGCCTGACAGCCAGGCGGTCATGGCGGCGTTCTGCCTGTGTCATGAGACGTGCTCCGCGAAAGTTATCTGACTGAAAATGATGTGATTCCTTGGAAGCGGGGGTCGTAGAGGGTTTGGGCACCGGGGAAACt < 1:672548/151‑1 (MQ=14) gCTGATTATCAGTGACAGCCTGACAGCCAGCCGGTCATGGCGGCGTTCTGCCTGTGTCATGAGACGTTCTCCGTGAAAGTTCACTGACTGAAAATGATGTGATTACTTTAAAGAGGGGGTCGGACAGGGTATGGACACCACAGAAACTAtt < 1:437159/151‑1 (MQ=255) gATTATCAGTGACAGCCTGACAGCCAGCCGGTCATGGCGGCGTTCTGCCTGTGTCATGAGACGTTCTCCGTGAAAGTTCACTGACTGAAAATGATGTGATTACTTTAAAGAGGGGGTCGGACAGGGTATGGACACCACAGAAACTAtttt < 1:220124/150‑1 (MQ=255) tATCAGTGACAGCCTGACAGCCAGCCGGTCATGGCGGCGTTCTGCCTGTGTCATGAGACGTTCTCCGTGAAAGTTCACTGACTGAAAATGATGTGATTACTTTAAAGAGGGGGTCGGACAGGGTATGGACACCACAGAAACTATTTTTCa < 1:228567/150‑1 (MQ=255) tATCAGTGACAGCCTGACAGCCAGCCGGTCATGGCGGCGTTCTGCCTGTGTCATGAGACGTTCTCCGTGAAAGTTCACTGACTGAAAATGATGTGATTACTTTAAAGAGGGGGTCGGACAGGGTATGGACACCACAGAAACTATTTTTCa < 1:203300/150‑1 (MQ=255) aTCAGTGACAGCCTGACAGCCAGCCGGTCATGGCGGCGTTCTGCCTGTGTCATGAGACGTTCTCCGTGAAAGTTCACTGACTGAAAATGATGTGATTACTTTAAAGAGGGGGTCGGACAGGGTATGGACACCACAGAAACTATTTTTCAtt > 2:440077/1‑151 (MQ=255) aTCAGTGACAGCCTGACAGCCAGCCGGTCATGGCGGCGTTCTGCCTGTGTCATGAGACGTTCTCCGTGAAAGTTCACTGACTGAAAATGATGTGATTACTTTAAAGAGGGGGTCGGACAGGGTATGGACACCACAGAAACTATTTTTCAt < 1:534004/150‑1 (MQ=255) cAGTGACAGCCTGACAGCCAGCCGGTCATGGCGGCGTTCTGCCTGTGTCATGAGACGTTCTCCGTGAAAGTTCACTGACTGAAAATGATGTGATTACTTTAAAGAGGGGGTCGGACAGGGTATGGACACCACAGAAACTATTTTTCATTTc > 2:843616/1‑151 (MQ=255) gacagcctgacagccAGCCGGTCATGGCGGCGTTCTGCCTGTGTCATGAGACGTTCTCCGTGAAAGTTCACTGACTGAAAATGATGTGATTACTTTAAAGAGGGGGTCGGACAGGGTATGGACACCACAGAAACTATTTTTCATTTCTGc < 2:302926/150‑1 (MQ=255) cagcctgacagccAGCCGGTCATGGCGGCGTTCTGCCTGTGTCATGAGACGTTCTCCGTGAAAGTTCACTGACTGAAAATGATGTGATTACTTTAAAGAGGGGGTCGGACAGGGTATGGACACCACAGAAACTATTTTTCATTTCTGCaaa < 2:122309/151‑1 (MQ=255) agcctgacagccAGCCGGTCATGGCGGCGTTCTGCCTGTGTCATGAGACGTTCTCCGTGAAAGTTCACTGACTGAAAATGATGTGATTACTTTAAAGAGGGGGTCGGACAGGGTATGGACACCACAGAAACTATTTTTCATTTCTGCaaaa < 2:4560/151‑1 (MQ=255) gcctgacagccAGCCGGTCATGGCGGCGTTCTGCCTGTGTCATGAGACGTTCTCCGTGAAAGTTCACTGACTGAAAATGATGTGATTACTTTAAAGAGGGGGTCGGACAGGGTATGGACACCACAGAAACTATTTTTCATTTCTGCaaa > 1:261788/1‑149 (MQ=255) gcctgacagccAGCCGGTCATGGCGGCGTTCTGCCTGTGTCATGAGACGTTCTCCGTGAAAGTTCACTGACTGAAAATGATGTGATTACTTTAAAGAGGGGGTCGGACAGGGTATGGACACCACAGAAACTATTTTTCATTTCTGCAAAAg < 1:180043/151‑1 (MQ=255) ccagccGGTCATGGCGGCGTTCTGCCTGTGTCATGAGACGTTCTCCGTGAAAGTTCACTGACTGAAAATGATGTGATTACTTTAAAGAGGGGGTCGGACAGGGTATGGACACCACAGAAACTATTTTTCATTTCTGCAAAAGCCAGAGGCa < 2:299498/151‑1 (MQ=255) cagccGGTCATGGCGGCGTTCTGCCTGTGTCATGAGACGTTCTCCGTGAAAGTTCACTGACTGAAAATGATGTGATTACTTTAAAGAGGGGGTCGGACAGGGTATGGACACCACAGAAACTATTTTTCATTTCTGCAAAAGCCAGAGGCAg < 2:107050/151‑1 (MQ=255) ggTCATGGCGGCGTTCTGCCTGTGTCATGAGACGTTCTCCGTGAAAGTTCACTGACTGAAAATGATGTGATTACTTTAAAGAGGGGGTCGGACAGGGTATGGACACCACAGAAACTATTTTTCATTTCTGCAAAAGCCAGAGGCAGCgggg > 1:13248/1‑151 (MQ=255) tCATGGCGGCGTTCTGCCTGTGTCATGAGACGTTCTCCGTGAAAGTTCACTGACTGAAAATGATGTGATTACTTTAAAGAGGGGGTCGGACAGGGTATGGACACCACAGAAACTATTTTTCATTTCTGCAAAAGCCAGAGGCAGCggggg > 1:689717/1‑150 (MQ=255) cATGGCGGCGTTCTGCCTGTGTCATGAGACGTTCTCCGTGAAAGTTCACTGACTGAAAATGATGTGATTACTTTAAAGAGGGGGTCGGACAGGGTATGGACACCACAGAAACTATTTTTCATTTCTGCAAAAGCCAGAGGCAGCGGGGGtt < 1:265044/151‑1 (MQ=255) gCCTGTGTCATGAGACGTTCTCCGTGAAAGTTCACTGACTGAAAATGATGTGATTACTTTAAAGAGGGGGTCGGACAGGGTATGGACACCACAGAAACTATTTTTCATTTCTGCAAAAGCCAGAGGCAGCGGGGGTTACAGGCCTTcc < 1:295244/148‑1 (MQ=255) tgtCATGAGACGTTCTCCGTGAAAGTTCACTGACTGAAAATGATGTGATTACTTTAAAGAGGGGGTCGGACAGGGTATGGACACCACAGAAACTATTTTTCATTTCTGCAAAAGCCAGAGGCAGCGGGGGTTACAGGCCTTCCCTCGgtg > 1:315630/1‑150 (MQ=255) cATGAGACGTTCTCCGTGAAAGTTCACTGACTGAAAATGATGTGATTACTTTAAAGAGGGGGTCGGACAGGGTATGGACACCACAGAAACTATTTTTCATTTCTGCAAAAGCCAGAGGCAGCGGGGGTTACAGGCCTTCCCTCGgtgagtg > 2:906797/1‑151 (MQ=255) cATGAGACGTTCTCCGTGAAAGTTCACTGACTGAAAATGATGTGATTACTTTAAAGAGGGGGTCGGACAGGGTATGGACACCACAGAAACTATTTTTCATTTCTGCAAAAGCCAGAGGCAGCGGGGGTTACAGGCCTTCCCTCGgtgagtg < 1:184544/151‑1 (MQ=255) cATGAGACGTTCTCCGTGAAAGTTCACTGACTGAAAATGATGTGATTACTTTAAAGAGGGGGTCGGACAGGGTATGGACACCACAGAAACTATTTTTCATTTCTGCAAAAGCCAGAGGCAGCGGGGGTTACAGGCCTTCCCTCGgtgagtg > 1:720620/1‑151 (MQ=255) cATGAGACGTTCTCCGTGAAAGTTCACTGACTGAAAATGATGTGATTACTTTAAAGAGGGGGTCGGACAGGGTATGGACACCACAGAAACTATTTTTCATTTCTGCAAAAGCCAGAGGCAGCGGGGGTTACAGGCCTTCCCTCGgtgagt < 2:757557/150‑1 (MQ=255) aTGAGACGTTCTCCGTGAAAGTTCACTGACTGAAAATGATGTGATTACTTTAAAGAGGGGGTCGGACAGGGTATGGACACCACAGAAACTATTTTTCATTTCTGCAAAAGCCAGAGGCAGCGGGGGTTACAGGCCTTCCCTCGgtgagtg > 2:445534/1‑150 (MQ=255) gagaCGTTCTCCGTGAAAGTTCACTGACTGAAAATGATGTGATTACTTTAAAGAGGGGGTCGGACAGGGTATGGACACCACAGAAACTATTTTTCATTTCTGCAAAAGCCAGAGGCAGCGGGGGTTACAGGCCTTCCCTCGGTGAGTGAgg < 1:229492/151‑1 (MQ=255) agaCGTTCTCCGTGAAAGTTCACTGACTGAAAATGATGTGATTACTTTAAAGAGGGGGTCGGACAGGGTATGGACACCACAGAAACTATTTTTCATTTCTGCAAAAGCCAGAGGCAGCGGGGGTTACAGGCCTTCCCTCGGTGAGTGAGGa < 2:154730/151‑1 (MQ=255) aCGTTCTCCGTGAAAGTTCACTGACTGAAAATGATGTGATTACTTTAAAGAGGGGGTCGGACAGGGTATGGACACCACAGAAACTATTTTTCATTTCTGCAAAAGCCAGAGGCAGCGGGGGTTACAGGCCTTCCCTCGGTGAGTGAGGAcc < 1:440062/151‑1 (MQ=255) gTTCTCCGTGAAAGTTCACTGACTGAAAATGATGTGATTACTTTAAAGAGGGGGTCGGACAGGGTATGGACACCACAGAAACTATTTTTCATTTCTGCAAAAGCCAGAGGCAGCGGGGGTTACAGGCCTTCCCTCGGTGAGTGAGGACCGt > 1:650954/1‑151 (MQ=255) ttCTCCGTGAAAGTTCACTGACTGAAAATGATGTGATTACTTTAAAGAGGGGGTCGGACAGGGTATGGACACCACAGAAACTATTTTTCATTTCTGCAAAAGCCAGAGGCAGCGGGGGTTACAGGCCTTCCCTCGGTGAGTGAGGACCGTc > 2:282398/1‑151 (MQ=255) ccGTGAAAGTTCACTGACTGAAAATGATGTGATTACTTTAAAGAGGGGGTCGGACAGGGTATGGACAACACAGAAACTATTTTTCATTTCTGCAAAAGCCAGAGGCAGCGGGGGTTACAGGCCTTCCCTCGGTGAGTGAGGACCGTCa < 1:33824/148‑1 (MQ=255) gTGAAAGTTCACTGACTGAAAATGATGTGATTACTTTAAAGAGGGGGTCGGACAGGGTATGGACACCACAGAAACTATTTTTCATTTCTGCAAAAGCCAGAGGCAGCGGGGGTTACAGGCCTTCCCTCGGTGAGTGAGGACCGTCAGCACg > 1:771263/1‑151 (MQ=255) aaaGTTCACTGACTGAAAATGATGTGATTACTTTAAAGAGGGGGTCGGACAGGGTATGGACACCACAGAAACTATTTTTCATTTCTGCAAAAGCCAGAGGCAGCGGGGGTTACAGGCCTTCCCTCGGTGAGTGAGGACCGTCAGCACGGTg > 2:563474/1‑151 (MQ=255) | CGCACCGACACACCAAACTCAGCGGCAAGCTTACGCACACTCAGCGTTTCCCCTGCCACCAGACGGCTGATTATCAGTGACAGCCTGACAGCCAGCCGGTCATGGCGGCGTTCTGCCTGTGTCATGAGACGTTCTCCGTGAAAGTTAACTGACTGAAAATGATGTGATTACTTTAAAGAGGGGGTCGGACAGGGTATGGACACCACAGAAACTATTTTTCATTTCTGCAAAAGCCAGAGGCAGCGGGGGTTACAGGCCTTCCCTCGGTGAGTGAGGACCGTCAGCACGGTG > NC_000913/266843‑267133 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |