Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	353,745	A→C	E347A (GAA→GCA)	prpE →	propionate‑‑CoA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	353,745	0	A	C	100.0%	201.3 / NA	55	E347A (GAA→GCA)	prpE	propionate‑‑CoA ligase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (31/24);  total (31/24)

GGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCCGGACTGCGGCGTGTGGTGGACAATCGTCGAGAAATATCAGGTTAGCCGGATGTTCTCAGCGCCGACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGACCGCCAGTTGGGTGAGCAATACGCTGGATGTGCCGGTCATCGACAACTACTGGCAGACCGAATCCGGC  >  NC_000913/353604‑353881                                                                                                                                              |                                                                                                                                         ggATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCCGGACTGCGGCGTGTGGTGGACAATCGTCGAGAAATATCAGGTTAGCCGGATGTTCTCAGCGCCGACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGCAATTCGCa                                                                                                                                  >  2:328843/1‑150 (MQ=255) ggATGGCGACTATCGTTTACGAAGGATTGCCGACCTGCCCGGACTGCGGCGTGTGGTGGACAAGCGTCGAGAAATATAAGGTTAGCCGGATGTTCCCAGCGCCGACCGCCATTCGCGTGCTGAAAAAATTCCCTCCCGCTGCAATTCGCaa                                                                                                                                 <  1:375099/151‑1 (MQ=255)     ggCGACTATCGTTTACGAAGGATTGCCGACCTGGCCGGACTGCGGCGTGTGGTGGACAATCGTCGAGAAATATCAGGTTAGCCGGATGTTCTCAGCGCCGACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGCAATTCGCAAacac                                                                                                                             >  2:261628/1‑151 (MQ=255)            aTCGTTTACGAAGGATTGCCGACCTGGCCGGACTGCGGCGTGTGGTGGACAATCGTCGAGAAATATCAGGTTAGCCGGATGTTCTCAGCGCCGACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGCAATTCGCAAACACGAtctct                                                                                                                      <  2:152680/151‑1 (MQ=255)            aTCGTTTACGAAGGATTGCCGACCTGGCCGGACTGCGGCGTGTGGTGGACAATCGTCGAGAAATATCAGGTTAGCCGGATGTTCTCAGCGCCGACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGCAATTCGCAAACACGAtctct                                                                                                                      >  1:162445/1‑151 (MQ=255)              cGTTTACGAAGGATTGCCGACCTGGCCGGACTGCGGCGTGTGGTGGACAATCGTCGAGAAATATCAGGTTAGCCGGATGTTCTCAGCGCCGACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGCAATTCGCAAACACGAtctctc                                                                                                                     <  2:679982/150‑1 (MQ=255)                 ttACGAAGGATTGCCGACCTGGCCGGACTGCGGCGTGTGGTGGACAATCGTCGAGAAATATCAGGTTAGCCGGATGTTCTCAGCGCCGACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGCAATTCGCAAACACGATCTCtcgtc                                                                                                                  <  1:803968/150‑1 (MQ=255)                  tACGAAGGATTGCCGACCTGGCCGGACTGCGGCGTGTGGTGGACAATCGTCGAGAAATATCAGGTTAGCCGGATGTTCTCAGCGCCGACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGCAATTCGCAAACACGATCTCTCGTCGc                                                                                                                >  1:302628/1‑151 (MQ=255)                     gAAGGATTGCCGACCTGGCCGGACTGCGGCGTGTGGTGGACAATCGTCGAGAAATATCAGGTTAGCCGGATGTTCTCAGCGCCGACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGCAATTCGCAAACACGATCTCTCGTCGCTg                                                                                                              <  1:484031/150‑1 (MQ=255)                           ttGCCGACCTGGCCGGACTGCGGCGTGTGGTGGACAATCGTCGAGAAATATCAGGTTAGCCGGATGTTCTCAGCGCCGACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGCAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGc                                                                                                       >  2:379419/1‑151 (MQ=255)                                     ggCCGGACTGCGGCGTGTGGTGGACAATCGTCGAGAAATATCAGGTTAGCCGGATGTTCTCAGCGCCGACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGCAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATctgg                                                                                              <  2:756645/150‑1 (MQ=255)                                     ggCCGGACTGCGGCGTGTGGTGGACAATCGTCGAGAAATATCAGGTTAGCCGGATGTTCTCAGCGCCGACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGCAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATctg                                                                                               <  1:399611/149‑1 (MQ=255)                                      gCCGGACTGCGGCGTGTGGTGGACAATCGTCGAGAAATATCAGGTTAGCCGGATGTTCTCAGCGCCGACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGCAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATctggct                                                                                            <  2:903002/151‑1 (MQ=255)                                         ggACTGCGGCGTGTGGTGGACAATCGTCGAGAAATATCAGGTTAGCCGGATGTTCTCAGCGCCGACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGCAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATctggctgg                                                                                          >  1:685873/1‑150 (MQ=255)                                             tGCGGCGTGTGGTGGACAATCGTCGAGAAATATCAGGTTAGCCGGATGTTCTCAGCGCCGACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGCAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAAc                                                                                     >  2:331456/1‑151 (MQ=255)                                                ggcgTGTGGTGGACAATCGTCGAGAAATATCAGGTTAGCCGGATGTTCTCAGCGCCGACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGCAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGc                                                                                  >  1:16215/1‑151 (MQ=255)                                                ggcgTGTGGTGGACAATCGTCGAGAAATATCAGGTTAGCCGGATGTTCTCAGCGCCGACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGCAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGc                                                                                  >  1:11424/1‑151 (MQ=255)                                                   gtgtGGTGGACAATCGTCGAGAAATATCAGGTTAGCCGGATGTTCTCAGCGCCGACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGCAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTg                                                                                <  2:841933/150‑1 (MQ=255)                                                       ggtggACAATCGTCGAGAAATATCAGGTTAGCCGGATGTTCTCAGCGCCGACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGCAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACg                                                                            <  1:911069/150‑1 (MQ=255)                                                        gtggACAATCGTCGAGAAATATCAGGTTAGCCGGATGTTCTCAGCGCCGACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGCAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACg                                                                            >  1:387589/1‑149 (MQ=255)                                                         tggACAATCGTCGAGAAATATCAGGTTAGCCGGATGTTCTCAGCGCCGACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGCAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAg                                                                          >  1:67362/1‑150 (MQ=255)                                                          ggACAATCGTCGAGAAATATCAGGTTAGCCGGATGTTCTCAGCGCCGACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGCAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGcc                                                                        >  1:901217/1‑151 (MQ=255)                                                              aaTCGTCGAGAAATATCAGGTTAGCCGGATGTTCTCAGCGCCGACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGCAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGAcc                                                                    <  1:573167/151‑1 (MQ=255)                                                                 cgtcgAGAAATATCAGGTTAGCCGGATGTTCTCAGCGCCGACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGCAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGACCGc                                                                  <  1:764832/150‑1 (MQ=255)                                                                  gtcgAGAAATATCAGGTTAGCCGGATGTTCTCAGCGCCGACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGCAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGACCGcc                                                                 >  1:478124/1‑150 (MQ=255)                                                                   tcgAGAAATATCAGGTTAGCCGGATGTTCTCAGCGCCGACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGCAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGACCGcc                                                                 >  2:335732/1‑149 (MQ=255)                                                                      aaaaaTAGCAGGTTGGCAGGTTGTCCCCGGCGCCGCCCGCCATTCGCGTGCTGAAAAAATCCCCTACCGCTGCAATTCGCAACCACGTTCTCTCGCCGCTGAAAGTGCTCTATCTGGCTGGAGACCCGCTGGACGAGCAGACCGCCAGt                                                              <  1:262826/147‑1 (MQ=255)                                                                       gaAATATCAGGTTAGCCGGATGTTCTCAGCGCCGACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGCAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGACCGCCAGTTgg                                                           >  2:55875/1‑151 (MQ=255)                                                                       gaAATATCAGGTTAGCCGGATGTTCTCAGCGCCGACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGCAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGACCGCCAGTTgg                                                           >  1:901521/1‑151 (MQ=255)                                                                         aaTATCAGGTTAGCCGGATGTTCTCAGCGCCGACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGCAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGACCGCCAGTTGGGt                                                         >  2:698013/1‑151 (MQ=255)                                                                           tatCAGGTTAGCCGGATGTTCTCAGCGCCGACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGCAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGACCGCCAGTTGGGTg                                                        >  2:309795/1‑150 (MQ=255)                                                                            atCAGGTTGGCCGAATGTCCCCAGCGCCGGCCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGCAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGACCGCCAGTTGGGTGa                                                       <  1:599288/150‑1 (MQ=255)                                                                                    aGCCGGATGTTCTCAGCGCCGACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGCAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGACCGCCAGTTGGGTGAGCAATACg                                               <  2:710457/150‑1 (MQ=255)                                                                                     gCCGGATGTTCTCAGCGCCGACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGCAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGACCGCCAGTTGGGTGAGCAATACg                                               <  1:377879/149‑1 (MQ=255)                                                                                     gCCGGATGTTCTCAGCGCCGACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGCAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGACCGCCAGTTGGGTGAGCAATACGCt                                             >  1:528443/1‑151 (MQ=255)                                                                                      ccGGATGTTCTCAGCGCCGACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGCAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGACCGCCAGTTGGGTGAGCAATACGCTg                                            >  2:181615/1‑151 (MQ=255)                                                                                         gATGTTCTCAGCGCCGACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGCAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGACCGCCAGTTGGGTGAGCAATACGCTGGAt                                         <  2:162448/151‑1 (MQ=255)                                                                                                  aGCGCCGACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGCAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGACCGCCAGTTGGGTGAGCAATACGCTGGATGTGCCGGTc                                >  1:126302/1‑151 (MQ=255)                                                                                                     gcCGACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGCAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGACCGCCAGTTGGGTGAGCAATACGCTGGATGTGCCGGTCATc                             >  2:195617/1‑151 (MQ=255)                                                                                                      ccGGCCGCCATTCGCGTGCTGAAAAAATCCCCTACCGCTGCAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGACCGCCAGTTGGGTGAGCAATACGCTGGATGTGCCGGTCATCg                            <  1:861652/151‑1 (MQ=255)                                                                                                      ccGACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGCAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGACCGCCAGTTGGGTGAGCAATACGCTGGATGTGCCGGTCATCg                            >  2:89685/1‑151 (MQ=255)                                                                                                      ccGACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGCAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGACCGCCAGTTGGGTGAGCAATACGCTGGATGTGCCGGTCATCg                            <  2:65715/151‑1 (MQ=255)                                                                                                          ccGCCATGCGCTTGCTGAAAAAATTCCCTGCCGCTGCAATTCGCAACCCCGGTCGCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGACCGCCAGTTGGGTGAGCAATACGCTGGATGTGCCGGTCATCGACaa                        <  1:257213/151‑1 (MQ=255)                                                                                                           cGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGCAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGACCGCCAGTTGGGTGAGCAATACGCTGGATGTGCCGGTCATCGACAac                       >  1:463626/1‑151 (MQ=255)                                                                                                              cATTCGCGTGCTGAAAAAATTCCCTACCGCTGCAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACAGCTGGACGAGCCGACCGCCAGTTGGGTGAGCAATACGCTGGATGTGCCGGTCATCGACAactac                    >  2:13584/1‑151 (MQ=255)                                                                                                               aTTCGCGTGCTGAAAAAATTCCCTACCGCTGCAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGACCGCCAGTTGGGTGAGCAATACGCTGGATGTGCCGGTCATCGACAactact                   >  2:115975/1‑151 (MQ=255)                                                                                                                   gcgTGCTGAAAAAATTCCCTACCGCTGCAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGACCGCCAGTTGGGTGAGCAATACGCTGGATGTGCCGGTCATCGACAACTACTGGCa               <  2:348877/151‑1 (MQ=255)                                                                                                                    cgTGCTGAAAAAATTCCCTACCGCTGCAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGACCGCCAGTTGGGTGAGCAATACGCTGGATGTGCCGGTCATCGACAACTACTGGCAg              <  1:266193/151‑1 (MQ=255)                                                                                                                          gAAAAAATTCCCTACCGCTGCAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGACCGCCAGTTGGGTGAGCAATACGCTGGATGTGCCGGTCATCGACAACTACTGGCAGACCg          <  2:308904/149‑1 (MQ=255)                                                                                                                           aaaaaaTTCCCTACCGCTGCAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGACCGCCAGTTGGGTGAGCAATACGCTGGATGTGCCGGTCATCGACAACTACTGGCAGACCGAAt       >  2:172662/1‑151 (MQ=255)                                                                                                                             aaaaTTCCCTACCGCTGCAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGACCGCCAGTTGGGTGAGCAATACGCTGGATGTGCCGGTCATCGACAACTACTGGCAGACCGAATc      >  1:827516/1‑150 (MQ=255)                                                                                                                             aaaaTTCCCTACCGCTGCAATTCGCAAACACGATCTCCCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGACCGCCAGTTGGGTGAGCAATACGCTGGATGTGCCGGTCATCGACAACTACTGGCAGACCGAATcc     <  1:856633/151‑1 (MQ=255)                                                                                                                              aaaTTCCCTACCGCTGCAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGACCGCCAGTTGGGTGAGCAATACGCTGGATGTGCCGGTCATCGACAACTACTGGCAGACCGAATCCg    >  2:61721/1‑151 (MQ=255)                                                                                                                               aaTTCCCTACCGCTGCAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGACCGCCAGTTGGGTGAGCAATACGCTGGATGTGCCGGTCATCGACAACTACTGGCAGACCGAATCCgg   >  1:626985/1‑151 (MQ=255)                                                                                                                                aTTCCCTACCGCTGCAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGACCGCCAGTTGGGTGAGCAATACGCTGGATGTGCCGGTCATCGACAACTACTGGCAGACCGAATCCGGc  >  2:805350/1‑151 (MQ=255)                                                                                                                                              |                                                                                                                                         GGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCCGGACTGCGGCGTGTGGTGGACAATCGTCGAGAAATATCAGGTTAGCCGGATGTTCTCAGCGCCGACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGACCGCCAGTTGGGTGAGCAATACGCTGGATGTGCCGGTCATCGACAACTACTGGCAGACCGAATCCGGC  >  NC_000913/353604‑353881

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑