Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	431,697	A→C	F106C (TTC→TGC)	tsx ←	nucleoside channel, receptor of phage T6 and colicin K

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	431,697	0	A	C	100.0%	192.9 / NA	52	F106C (TTC→TGC)	tsx	nucleoside channel, receptor of phage T6 and colicin K
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (23/29);  total (23/29)

GATATCGGTACCCAGACCCATGTACCAGGTGCTCTGGCGACCATCTTTATTACGACCCATGTCGTAAATGTAGTTGTTCGCGAAGTACCACTCTTTGAACGGACCGAAGCTAAGGTCAGTATTGGTCAGCTTGTCGATGGAGAAACGTGGTTCGATTTCCATAAACAGCGGAGAACCGTGGTTCCAGATACCTTTAGCATCGGAGTTACCGCCGAAGAATACCGGCGCATCCGCATAACCATAGAAGTCGAACCAGTCTTTTTTAGCGAATGCTTCGTACTCAAGGTAGG  >  NC_000913/431555‑431844                                                                                                                                               |                                                                                                                                                    gATATCGGTACCCAGACCCATGTACCAGGTGCTCTGGCGACCATCTTTATTACGACCCATGTCGTAAATGTAGTTGTTCGCGAAGTACCACTCTTTGAACGGACCGAAGCTAAGGTCAGTATTGGTCAGCTTGTCGATGGAGCAACGTGGt                                                                                                                                             >  1:931261/1‑151 (MQ=255)    atCGGTACCCAGACCCATGTACCAGGTGCTCTGGCGACCATCTTTATTACGACCCATGTCGTAAATGTAGTTGTTCGCGAAGTACCACTCTTTGAACGGACCGAAGCTAAGGTCAGTATTGGTCAGCTTGTCGATGGAGCAACGTGGTTc                                                                                                                                           <  2:565016/150‑1 (MQ=255)          aCCCAGACCCATGTACCAGGTGCTCTGGCGACCATCTTTATTACGACCCATGTCGTAAATGTAGTTGTTCGCGAAGTACCACTCTTTGAACGGACCGAAGCTAAGGTCAGTATTGGTCAGCTTGTCGATGGAGCAACGTGGTTCGATTTc                                                                                                                                     <  2:589682/150‑1 (MQ=255)            ccAGACCCATGTACCAGGTGCTCTGGCGACCATCTTTATTACGACCCATGTCGTAAATGTAGTTGTTCGCGAAGTACCACTCTTTGAACGGACCGAAGCTAAGGTCAGTATTGGTCAGCTTGTCGATGGAGCAACGTGGTTCGATTTCCAt                                                                                                                                  <  1:531868/151‑1 (MQ=255)              aGACCCATGTACCAGGTGCTCTGGCGACCATCTTTATTACGACCCATGTCGTAAATGTAGTTGTTCGCGAAGTACCACTCTTTGAACGGACCGAAGCTAAGGTCAGTATTGGTCAGCTTGTCGATGGAGCAACGTGGTTCGATTTCCATaa                                                                                                                                <  2:425525/151‑1 (MQ=255)              aGACCCATGTACCAGGTGCTCTGGCGACCATCTTTATTACGACCCATGTCGTAAATGTAGTTGTTCGCGAAGTACCACTCTTTGAACGGACCGAAGCTAAGGTCAGTATTGGTCAGCTTGTCGATGGAGCAACGTGGTTCGATTTCCATa                                                                                                                                 >  2:631484/1‑150 (MQ=255)                  ccATGTACCAGGTGCTCTGGCGACCATCTTTATTACGACCCATGTCGTAAATGTAGTTGTTCGCGAAGTACCACTCTTTGAACGGACCGAAGCTAAGGTCAGTATTGGTCAGCTTGTCGATGGAGCAACGTGGTTCGATTTCCATAAACAg                                                                                                                            <  1:72972/151‑1 (MQ=255)                    aTGTATCAGGTGCTCTGGCGACCATCTTTATTACGACCCATGTCGTAAATGTAGTTGTTCGCGAAGTACCACTCTTTGAACGGACCGAAGCTAAGGTCAGTATTGGTCAGCTTGTCGATGGAGCAACGTGGTTCGATTTCCATAAACAGCg                                                                                                                          >  1:379039/1‑151 (MQ=255)                          cAGGTGCTCTGGCGACCATCTTTATTACGACCCATGTCGTAAATGTAGTTGTTCGCGAAGTACCACTCTTTGAACGGACCGAAGCTAAGGTCAGTATTGGTCAGCTTGTCGATGGAGCAACGTGGTTCGATTTCCATAAACAGCGGAGAAc                                                                                                                    >  1:55388/1‑151 (MQ=255)                           aGGTGCTCTGGCGACCATCTTTATTACGACCCATGTCGTAAATGTAGTTGTTCGCGAAGTACCACTCTTTGAACGGACCGAAGCTAAGGTCAGTATTGGTCAGCTTGTCGATGGAGCAACGTGGTTCGATTTCCATAAACAGCGGAGAAcc                                                                                                                   <  1:179592/151‑1 (MQ=255)                            ggTGCTCTGGCGACCATCTTTATTACGACCCATGTCGTAAATGTAGTTGTTCGCGAAGTACCACTCTTTGAACGGACCGAAGCTAAGGTCAGTATTGGTCAGCTTGTCGATGGAGCAACGTGGTTCGATTTCCATAAACAGCGGAGAACCg                                                                                                                  <  1:651312/151‑1 (MQ=255)                              tGCTCTGGCGACCATCTTTATTACGACCCATGTCGTAAATGTAGTTGTTCGCGAAGTACCACTCTTTGAACGGACCGAAGCTAAGGTCAGTATTGGTCAGCTTGTCGATGGAGCAACGTGGTTCGATTTCCATAAACAGCGGAGAACCGt                                                                                                                 >  2:590829/1‑150 (MQ=255)                              tGCTCTGGCGACCATCTTTATTACGACCCATGTCGTAAATGTAGTTGTTCGCGAAGTACCACTCTTTGAACGGACCGAAGCTAAGGTCAGTATTGGTCAGCTTGTCGATGGAGCAACGTGGTTCGATTTCCATAAACAGCGGAGAACCGTg                                                                                                                >  1:824176/1‑151 (MQ=255)                                         ccATCTTTATTACGACCCATGTCGTAAATGTAGTTGTTCGCGAAGTACCACTCTTTGAACGGACCGAAGCTAAGGTCAGTATTGGTCAGCTTGTCGATGGAGCAACGTGGTTCGATTTCCATAAACAGCGGAGAACCGTGGTTCCAGATAc                                                                                                     >  1:2424/1‑151 (MQ=255)                                               ttattaCGACCCATGTCGTAAATGTAGTTGTTCGCGAAGTACCACTCTTTGAACGGACCGAAGCTAAGGTCAGTATTGGTCAGCTTGTCGATGGAGCAACGTGGTTCGATTTCCATAAACAGCGGAGAACCGTGGTTCCAGATACCTTTa                                                                                                <  2:129660/150‑1 (MQ=255)                                                tattaCTACCCATGTCGTAAATGTAGTTGTTCGCGAAGTACCACTCGTTGAACGGACCGAAGCTAAGGTCAGTATTGGTCAGCTTGTCGATGGAGCAACGTGGTTCGATTTCCATAACCAGCGGAGAACCGTGGTTCCAGATTCCt                                                                                                   <  1:442586/146‑1 (MQ=255)                                                   taCGACCCATGTCGTAAATGTAGTTGTTCGCGAAGTACCACTCTTTGAACGGACCGAAGCTAAGGTCAGTATTGGTCAGCTTGTCGATGGAGCAACGTGGTTCGATTTCCATAAACAGCGGAGAACCGTGGTTCCAGATACCTTTAGCAt                                                                                            <  2:877979/150‑1 (MQ=255)                                                   taCGACCCATGTCGTAAATGTAGTTGTTCGCGAAGTACCACTCTTTGAACGGACCGAAGCTAAGGTCAGTATTGGTCAGCTTGTCGATGGAGCAACGTGGTTCGATTTCCATAAACAGCGGAGAACCGTGGTTCCAGATACCTTTAGCAt                                                                                            <  1:222669/150‑1 (MQ=255)                                                    aCGACCCATGTCGTAAATGTAGTTGTTCGCGAAGTACCACTCTTTGAACGGACCGAAGCTAAGGTCAGTATTGGTCAGCTTGTCGATGGAGCAACGTGGTTCGATTTCCATAAACAGCGGAGAACCGTGGTTCCAGATACCTTTAGCATCg                                                                                          <  2:206198/151‑1 (MQ=255)                                                     cGACCCATGTCGTAAATGTAGTTGTTCGCGAAGTACCACTCTTTGAACGGACCGAAGCTAAGGTCAGTATTGGTCAGCTTGTCGATGGAGCAACGTGGTTCGATTTCCATAAACAGCGGAGAACCGTGGTTCCAGATACCTTTAGCATCgg                                                                                         <  2:90886/151‑1 (MQ=255)                                                     cGACCCATGTCGTAAATGTAGTTGTTCGCGAAGTACCACTCTTTGAACGGACCGAAGCTAAGGTCAGTATTGGTCAGCTTGTCGATGGAGCAACGTGGTTCGATTTCCATAAACAGCGGAGAACCGTGGTTCCAGATACCTTTAGCATCgg                                                                                         <  1:191589/151‑1 (MQ=255)                                                           aTGTCGTAAATGTAGTTGTTCGCGAAGTACCACTCTTTGAACGGACCGAAGCTAAGGTCAGTATTGGTCAGCTTGTCGATGGAGCAACGTGGTTCGATTTCCATAAACAGCGGAGAACCGTGGTTCCAGATACCTTTAGCATCGGAGTTAc                                                                                   >  1:806026/1‑151 (MQ=255)                                                             gTCGTAAATGTAGTTGTTCGCGAAGTACCACTCTTTGAACGGACCGAAGCTAAGGTCAGTATTGGTCAGCTTGTCGATGGAGCAACGTGGTTCGATTTCCATAAACAGCGGAGAACCGTGGTTCCAGATACCTTTAGCATCGGAGTTAcc                                                                                  <  2:38837/150‑1 (MQ=255)                                                             gTCGTAAATGTAGTTGTTCGCGAAGTACCACTCTTTGAACGGACCGAAGCTAAGGTCAGTATTGGTCAGCTTGTCGATGGAGCAACGTGGTTCGATTTCCATAAACAGCGGAGAACCGTGGTTCCAGATACCTTTAGCATCGGAGTTAcc                                                                                  <  2:273111/150‑1 (MQ=255)                                                               cGTAAATGTAGTTGTTCGCGAAGTACCACTCTTTGAACGGACCGAAGCTAAGGTCAGTATTGGTCAGCTTGTCGATGGAGCAACGTGGTTCGATTTCCATAAACAGCGGAGAACCGTGGTTCCAGATACCTTTAGCATCGGAGTTAccgcc                                                                               <  1:502050/151‑1 (MQ=255)                                                               cGTAAATGTAGTTGTTCGCGAAGTACCACTCTTTGAACGGACCGAAGCTAAGGTCAGTATTGGTCAGCTTGTCGATGGAGCAACGTGGTTCGATTTCCATAAACAGCGGAGAACCGTGGTTCCAGATACCTTTAGCATCGGAGTTAccgcc                                                                               >  1:142306/1‑151 (MQ=255)                                                                gTAAATGTAGTTGTTCGCGAAGTACCACTCTTTGAACGGACCGAAGCTAAGGTCAGTATTGGTCAGCTTGTCGATGGAGCAACGTGGTTCGATTTCCATAAACAGCGGAGAACCGTGGTTCCAGATACCTTTAGCATCGGAGTTAccgccg                                                                              >  2:832162/1‑151 (MQ=255)                                                                  aaaTGTAGTTGTTCGCGAAGTACCACTCTTTGAACGGACCGAAGCTAAGGTCAGTATTGGTCAGCTTGTCGATGGAGCAACGTGGTTCGATTTCCATAAACAGCGGAGAACCGTGGTTCCAGATACCTTTAGCATCGGAGTTACCGCCgaa                                                                            <  2:43086/151‑1 (MQ=255)                                                                     tGTAGTTGTTCGCGAAGTACCACTCTTTGAACGGACCGAAGCTAAGGTCAGTATTGGTCAGCTTGTCGATGGAGCAACGTGGTTCGATTTCCATAAACAGCGGAGAACCGTGGTTCCAGATACCTTTAGCATCGGAGTTACCGCCgaag                                                                           >  2:631016/1‑149 (MQ=255)                                                                        aGTTGTTCGCGAAGTAGCACTCTTTGAACGGACCGAAGCTAAGGTCAGTATTGGTCAGCTTGTCGATGGAGCAACGTGGTTCGATTTCCATAAACAGCGGAGAACCGTGGTTCCAGATACCTTTAGCATCGGAGTTACCGCCGAAGAATAc                                                                      <  2:208220/151‑1 (MQ=255)                                                                           tgttCGCGAAGTACCACTCTTTGAACGGACCGAAGCTAAGGTCAGTATTGGTCAGCTTGTCGATGGAGCAACGTGGTTCGATTTCCATAAACAGCGGAGAACCGTGGTTCCAGATACCTTTAGCATCGGAGTTACCGCCGAAGAATACCgg                                                                   >  2:155373/1‑151 (MQ=255)                                                                            gttCGCGAAGTACCACTCTTTGAACGGACCGAAGCTAAGGTCAGTATTGGTCAGCTTGTCGATGGAGCAACGTGGTTCGATTTCCATAAACAGCGGAGAACCGTGGTTCCAGATACCTTTAGCATCGGAGTTACCGCCGAAGAATACCGgc                                                                  >  1:303548/1‑151 (MQ=255)                                                                            gttCGCGAAGTACCACTCTTTGAACGGACCGAAGCTAAGGTCAGTATTGGTCAGCTTGTCGATGGAGCAACGTGGTTCGATTTCCATAAACAGCGGAGAACCGTGGTTCCAGATACCTTTAGCATCGGAGTTACCGCCGAAGAATACCGgc                                                                  <  2:408825/151‑1 (MQ=255)                                                                                 cgAAGTACCACTCTTTGAACGGACCGAAGCTAAGGTCAGTATTGGTCAGCTTGTCGATGGAGCAACGTGGTTCGATTTCCATAAACAGCGGAGAACCGTGGTTCCAGATACCTTTAGCATCGGAGTTACCGCCGAAGAATACCGGCGCATc                                                             >  1:745711/1‑151 (MQ=255)                                                                                      tACCACTCTTTGAGCGGACCGAAGCTAAGGTCAGTATTGGTCAGCTTGTCGATGGAGCAACGTGGTTCGATTTACATAAACAGCGGAGAACCGTGGTTCCAGATACCTTTAGCATCGGAGTTACCGCCGAAGAATACCGGCGCATCCGCa                                                         <  2:158005/150‑1 (MQ=255)                                                                                               ttGAACGGACCGAAGCTAAGGTCAGTATTGGTCAGCTTGTCGATGGAGCAACGTGGTTCGATTTCCATAAACAGCGGAGAACCGTGGTTCCAGATACCTTTAGCATCGGAGTTACCGCCGAAGAATACCGGCGCATCCGCATAACCATAg                                                <  2:637454/150‑1 (MQ=255)                                                                                                    cGGACCGAAGCTAAGGTCAGTATTGGTCAGCTTGTCGATGGAGCAACGTGGTTCGATTTCCATAAACAGCGGAGAACCGTGGTTCCAGATACCTTTAGCATCGGAGTTACCGCCGAAGAATACCGGCGCATCCGCATAACCATAGAAGTc                                           <  1:761729/150‑1 (MQ=255)                                                                                                    cGGACCGAAGCTAAGGTCAGTATTGGTCAGCTTGTCGATGGAGCAACGTGGTTCGATTTCCATAAACAGCGGAGAACCGTGGTTCCAGATACCTTTAGCATCGGAGTTACCGCCGAAGAATACCGGCGCATCCGCATAACCATAGAAGTCg                                          <  2:402493/151‑1 (MQ=255)                                                                                                     ggACCGAAGCTAAGGTCAGTATTGGTCAGCTTGTCGATGGAGCAACGTGGTTCGATTTCCATAAACAGCGGAGAACCGTGGTTCCAGATACCTTTAGCATCGGAGTTACCGCCGAAGAATACCGGCGCATCCGCATAACCATAGAAGTCg                                          <  1:391054/150‑1 (MQ=255)                                                                                                         cGAAGCTAAGGTCAGTATTGGTCAGCTTGTCGATGGAGCAACGTGGTTCGATTTCCATAAACAGCGGAGAACCGTGGTTCCAGATACCTTTAGCATCGGAGTTACCGCCGAAGAATACCGGCGCATCCGCATAACCATAGAAGTCGAAcc                                      >  2:835987/1‑150 (MQ=255)                                                                                                         cGAAGCTAAGGTCAGTATTGGTCAGCTTGTCGATGGAGCAACGTGGTTCGATTTCCATAAACAGCGGAGAACCGTGGTTCCAGATACCTTTAGCATCGGAGTTACCGCCGAAGAATACCGGCGCATCCGCATAACCATAGAAGTCGAAcc                                      >  1:551488/1‑150 (MQ=255)                                                                                                         cGAAGCTAAGGTCAGTATTGGTCAGCTTGTCGATGGAGCAACGTGGTTCGATTTCCATAAACAGCGGAGAACCGTGGTTCCAGATACCTTTAGCATCGGAGTTACCGCCGAAGAATACCGGCGCATCCGCATAACCATAGAAGTCGAACCa                                     <  1:712374/151‑1 (MQ=255)                                                                                                           aaGCTAAGGTCAGTATTGGTCAGCTTGTCGATGGAGCAACGTGGTTCGATTTCCATAAACAGCGGAGAACCGTGGTTCCAGATACCTTTAGCATCGGAGTTACCGCCGAAGAATACCGGCGCATCCGCATAACCATAGAAGTCGAACCAGt                                   >  2:350538/1‑151 (MQ=255)                                                                                                            aGCTAAGGTCAGTATTGGTCAGCTTGTCGATGGAGCAACGTGGTTCGATTTCCATAAACAGCGGAGAACCGTGGTTCCAGATACCTTTAGCATCGGAGTTACCGCCGAAGAATACCGGCGCATCCGCATAACCATAGAAGTCGAACCAGTc                                  <  1:500003/151‑1 (MQ=255)                                                                                                               tAAGGTCAGTATTGGTCAGCTTGTCGATGGAGCAACGTGGTTCGATTTCCATAAACAGCGGAGAACCGTGGTTCCAGATACCTTTAGCATCGGAGTTACCGCCGAAGAATACCGGCGCATCCGCATAACCATAGAAGTCGAACCAGTCttt                               >  2:685673/1‑151 (MQ=255)                                                                                                               tAAGGTCAGTATTGGTCAGCTTGTCGATGGAGCAACGTGGTTCGATTTCCATAAACAGCGGAGAACCGTGGTTCCAGATACCTTTAGCATCGGAGTTACCGCCGAAGAATACCGGCGCATCCGCATAACCATAGAAGTCGAACCAGTCttt                               >  1:802727/1‑151 (MQ=255)                                                                                                                aaGGTCAGTATTGGTCAGCTTGTCGATGGAGCAACGTGGTTCGATTTCCATAAACAGCGGAGAACCGTGGTTCCAGATACCTTTAGCATCGGAGTTACCGCCGAAGAATACCGGCGCATCCGCATAACCATAGAAGTCGAACCAGTCtttt                              >  1:330468/1‑151 (MQ=255)                                                                                                                aaGGTCAGTATTGGTCAGCTTGTCGATGGAGCAACGTGGTTCGATTTCCATAAACAGCGGAGAACCGTGGTTCCAGATACCTTTAGCATCGGAGTTACCGCCGAAGAATACCGGCGCATCCGCATAACCATAGAAGTCGAACCAGTCtttt                              >  1:518159/1‑151 (MQ=255)                                                                                                                  ggTCAGTATTGGTCAGCTTGTCGATGGAGCAACGTGGTTCGATTTCCATAAACAGCGGAGAACCGTGGTTCCAGATACCTTTAGCATCGGAGTTACCGCCGAAGAATACCGGCGCATCCGCATAACCATAGAAGTCGAACCAGTCtttttt                            <  1:369727/151‑1 (MQ=255)                                                                                                                     cAGTATTGGTCAGCTTGTCGATGGAGCAACGTGGTTCGATTTCCATAAACAGCGGAGAACCGTGTTTCCAGATACCGTTAGCATCGGAGTTACCGCCGAAGAATACCGGCGCATCCGCATAACCATAGAAGTCGAACCAGTCTTTTTTAg                          <  1:739166/150‑1 (MQ=255)                                                                                                                           tGGTCAGCTTGTCGATGGAGCAACGTGGTTCGATTTCCATAAACAGCGGAGAACCGTGGTTCCAGATACCTTTAGCATCGGAGTTACCGCCGAAGAATACCGGCGCATCCGCATAACCATAGAAGTCGAACCAGTCTTTTTTAGCGAATGc                   >  1:558624/1‑151 (MQ=255)                                                                                                                               cAGCTTGTCGATGGAGCAACGTGGTTCGATTTCCATAAACAGCGGAGAACCGTGGTTCCAGATACCTTTAGCATCGGAGTTACCGCCGAAGAATACCGGCGCATCCGCATAACCATAGAAGTCGAACCAGTCTTTTTTAGCGAATGCTTCg               >  2:414596/1‑151 (MQ=255)                                                                                                                                            gagcAACGTGGTTCGATTTCCATAAACAGCGGAGAACCGTGGTTCCAGATACCTTTAGCATCGGAGTTACCGCCGAAGAATACCGGCGCATCCGCATAACCATAGAAGTCGAACCAGTCTTTTTTAGCGAATGCTTCGTACTCAaggtagg  >  1:886272/1‑151 (MQ=255)                                                                                                                                               |                                                                                                                                                    GATATCGGTACCCAGACCCATGTACCAGGTGCTCTGGCGACCATCTTTATTACGACCCATGTCGTAAATGTAGTTGTTCGCGAAGTACCACTCTTTGAACGGACCGAAGCTAAGGTCAGTATTGGTCAGCTTGTCGATGGAGAAACGTGGTTCGATTTCCATAAACAGCGGAGAACCGTGGTTCCAGATACCTTTAGCATCGGAGTTACCGCCGAAGAATACCGGCGCATCCGCATAACCATAGAAGTCGAACCAGTCTTTTTTAGCGAATGCTTCGTACTCAAGGTAGG  >  NC_000913/431555‑431844

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑