Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	435,380	A→C	K82Q (AAA→CAA)	nusB →	transcription antitermination protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	435,380	0	A	C	100.0%	239.9 / NA	64	K82Q (AAA→CAA)	nusB	transcription antitermination protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (39/25);  total (39/25)

GTTCCTGGCTGAACAGGATGTAAAAGACGTTGACGTCCTGTACTTCCGTGAGCTGCTGGCCGGGGTGGCGACTAATACCGCATACCTCGACGGACTGATGAAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAAAAAGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGTAGCGATGTGCCATACAAAGTGGCCATTAACGAAGCGATCGAACTGGCGAAATCGTTCGGCGCAGAAGACAGCCATAAGTTCGTCAACGGCGTACTCGATA  >  NC_000913/435235‑435521                                                                                                                                                  |                                                                                                                                              gTTCCTGGCTGAACAGGATGTAAAAGACGTTGACGTCCTGTACTTCCGTGAGCTGCTGGCCGGGGTGGCGACTAATACCGCATACCTCGACGGACTGATGAAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAACAAGc                                                                                                                                           >  2:12026/1‑150 (MQ=255) gTTCCTGGCTGAACAGGATGTAAAAGACGTTGACGTCCTGTACTTCCGTGAGCTGCTGGCCGGGGTGGCGACTAATACCGCATACCTCGACGGACTGATGAAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAACAAGCa                                                                                                                                          <  2:120257/151‑1 (MQ=255)   tCCTGGCTGAACAGGATGTAAAAGACGTTGACGTCCTGTACTTCCGTGAGCTGCTGGCCGGGGTGGCGACTAATACCGCATACCTCGACGGACTGATGAAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAACAAGCAg                                                                                                                                         <  2:224172/150‑1 (MQ=255)      tGGCTGAACAGGATGTAAAAGACGTTGACGTCCTGTACTTCCGTGAGCTGCTGGCCGGGGTGGCGACTAATACCGCATACCTCGACGGACTGATGAAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAACAAGCAGTACt                                                                                                                                     >  1:598088/1‑151 (MQ=255)      tGGCTGAACAGGATGTAAAAGACGTTGACGTCCTGTACTTCCGTGAGCTGCTGGCCGGGGTGGCGACTAATACCGCATACCTCGACGGACTGATGAAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAACAAGCAGTACt                                                                                                                                     >  2:162872/1‑151 (MQ=255)      tGGCTGAACAGGATGTAAAAGACGTTGACGTCCTGTACTTCCGTGAGCTGCTGGCCGGGGTGGCGACTAATACCGCATACCTCGACGGACTGATGAAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAACAAGCAGTACt                                                                                                                                     >  1:401620/1‑151 (MQ=255)         cTGAACAGGATGTAAAAGACGTTGACGTCCTGTACTTCCGTGAGCTGCTGGCCGGGGTGGCGACTAATACCGCATACCTCGACGGACTGATGAAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAACAAGCAGTACTgcg                                                                                                                                  >  1:832957/1‑151 (MQ=255)              cAGGATGTAAAAGACGTTGACGTCCTGTACTTCCGTGAGCTGCTGGCCGGGGTGGCGACTAATACCGCATACCTCGACGGACTGATGAAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAACAAGCAGTACTGCGCATTg                                                                                                                             >  2:37399/1‑151 (MQ=255)               aGGATGTAAAAGACGTTGACGACCTGTACTTCCGTGAGCTGCTGGCCGGGGTGGCGATTAATACCGCACGCCTCCGCGGTGTAATGAAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAACAAGCAGTACTGCGCATTgc                                                                                                                            <  2:365792/151‑1 (MQ=255)                ggATGTAAAAGACGTTGACGTCCTGTCCTTCCGTGAGCTGCTGGCCGGGGTGGCGACTAATACCGCATCCCTCGACGGACTGATGAAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAACAAGCAGTACTGCGCATTgcg                                                                                                                           <  2:107340/151‑1 (MQ=255)                ggATGTAAAAGACGTTGACGTCCTGTACTTCCGTGAGCTGCTGGCCGGGGTGGCGACTAATACCGCATACCTCGACGGACTGATGAAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAACAAGCAGTACTGCGCATTgcg                                                                                                                           >  1:854828/1‑151 (MQ=255)                ggATGTAAAAGACGTTGACGTCCTGTACTTCCGTGAGCTGCTGGCCGGGGTGGCGACTAATACCGCATACCTCGACGGACTGATGAAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAACAAGCAGTACTGCGCATTgcg                                                                                                                           >  2:723903/1‑151 (MQ=255)                   tGTAAAAGACGTTGACGTCCTGTACTTCCGTGAGCTGCTGGCCGGGGTGGCGACTAATACCGCATACCTCGACGGACTGATGAAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAACAAGCAGTACTGCGCATTGCGCTg                                                                                                                        >  1:897107/1‑151 (MQ=255)                      aaaaGACGTTGACGTCCTGTACTTCCGTGAGCTGCTGGCCGGGGTGGCGACTAATACCGCATACCTCGACGGACTGATGAAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAACAAGCAGTACTGCGCATTGCGCTGTa                                                                                                                      <  1:5053/150‑1 (MQ=255)                       aaaGACGTTGACGTCCTGTACTTCCGTGAGCTGCTGGCCGGGGTGGCGACTAATACCGCATACCTCGACGGACTGATGAAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAACAAGCAGTACTGCGCATTGCGCTGTACg                                                                                                                    >  1:823147/1‑151 (MQ=255)                        aaGACGTTGACGTCCTGTACTTCCGTGAGCTGCTGGCCGGGGTGGCGACTAATCCCGCATACCTCGACGGACTGATGAAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAACAAGCAGTACTGCGCATTGCGCTGTACGa                                                                                                                   <  1:434647/151‑1 (MQ=255)                           aCGTTGACGTCCTGTACTTCCGTGAGCTGCTGGCCGGGGTGGCGACTAATACCGCATACCTCGACGGACTGATGAAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAACAAGCAGTACTGCGCATTGCGCTGTACGAACt                                                                                                                >  1:145543/1‑151 (MQ=255)                              ttGACGTCCTGTACTTCCGTGAGCTGCTGGCCGGGGTGGCGACTAATACCGCATCCCTCGACGGACTGATGAAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAACAAGCAGTACTGCGCATTGCGCTGTACGAACt                                                                                                                <  1:94660/148‑1 (MQ=255)                              ttGACGTCCTGTACTTCCGTGAGCTGCTGGCCGGGGTGGCGACTAATACCGCATACCTCGACGGACTGATGAAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAACAAGCAGTACTGCGCATTGCGCTGTACGAACTGTc                                                                                                             >  2:449447/1‑151 (MQ=255)                                gACGTCCTGTACTTCCGTGAGCTGCTGGCCGGGGTGGCGACTAATACCGCATACCTCGACGGACTGATGAAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAACAAGCAGTACTGCGCATTGCGCTGTACGAACTGTCt                                                                                                            >  1:179657/1‑150 (MQ=255)                                  cGTCCTGTACTTCCGTGAGCTGCTGGCCGGGGTGGCGACTAATACCGCATACCTCGACGGACTGATGAAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAACAAGCAGTACTGCGCATTGCGCTGTACGAACTGTCTaa                                                                                                          >  2:670858/1‑150 (MQ=255)                                  cGTCCTGTACTTCCGTGAGCTGCTGGCCGGGGTGGCGACTAATACCGCATACCTCGACGGACTGATGAAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAACAAGCAGTACTGCGCATTGCGCTGTACGAACTGTCTaa                                                                                                          >  1:693184/1‑150 (MQ=255)                                   gTCCTGTACTTCCGTGAGCTGCTGGCCGGGGTGGCGACTAATACCGCATACCTCGACGGACTGATGAAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAACAAGCAGTACTGCGCATTGCGCTGTACGAACTGTCTaaa                                                                                                         >  1:861697/1‑150 (MQ=255)                                   gTCCTGTACTTCCGTGAGCTGCTGGCCGGGGTGGCGACTAATACCGCATACCTCGACGGACTGATGAAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAACAAGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAAc                                                                                                        >  1:771946/1‑151 (MQ=255)                                       tGTACTTCCGTGAGCTGCTGGCCGGGGTGGCGACTAATACCGCATACCTCGACGGACTGATGAAGCCATCCCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAACAAGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACg                                                                                                       <  2:377931/148‑1 (MQ=255)                                       tGTACTTCCGTGAGCTGCTGGCCGGGGTGGCGACTAATACCGCATACCTCGACGGACTGATGAAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAACAAGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGt                                                                                                      >  1:11348/1‑149 (MQ=255)                                        gTACTTCCGTGAGCTGCTGGCCGGGGTGGCGACTAATACCGCATACCTCGACGGACTGATGAAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAACAAGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGTa                                                                                                     <  2:359363/149‑1 (MQ=255)                                           cTTCCGTGAGCTGCTGGCCGGGGTGGCGACTAATACCGCATACCTCGACGGACTGATGAAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTATAACAAGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGTAGCGAt                                                                                                >  1:870215/1‑151 (MQ=255)                                           cTTCCGTGAGCTGCTGGCCGGGGTGGCGACTAATACCGCATACCTCGACGGACTGATGAAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAACAAGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGTAGCGAt                                                                                                >  2:28706/1‑151 (MQ=255)                                            ttCCGTGAGCTGCTGGCCGGGGTGGCGACTAATACCGCATACCTCGACGGACTGATGAAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAACAAGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGTAGCGAtg                                                                                               >  1:609239/1‑151 (MQ=255)                                            ttCCGTGAGCTGCTGGCCGGGGTGGCGACTAATACCGCATACCTCGACGGACTGATGAAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAACAAGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGTAGCGAtg                                                                                               >  2:643930/1‑151 (MQ=255)                                                gTGAGCTGCTGGCCGGGGTGGCGACTAATACCGCATACCTCGACGGACTGATGAAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAACAAGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGTAGCGATGTGcc                                                                                           >  2:169418/1‑151 (MQ=255)                                                gTGAGCTGCTGGCCGGGGTGGCGACTAATACCGCATACCTCGACGGACTGATGAAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAACAAGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGTAGCGATGTGcc                                                                                           >  1:247487/1‑151 (MQ=255)                                                     ctgctgGCCGGGGTGGCGACTAATACCGCATACCTCGACGGACTGATGAAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAACAAGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGTAGCGATGTGCCATAc                                                                                       >  1:595442/1‑150 (MQ=255)                                                            ccGGGGTGGCGACTAATACCGCATACCTCGACGGACTGATGAAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAACAAGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGTAGCGATGTGCCATACAAAGTgg                                                                                >  1:129414/1‑150 (MQ=255)                                                              ggggTGGCGACTAATACCGCATACCTCGACGGACTGATGAAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAACAAGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGTAGCGATGTGCCATACAAAGTGGcc                                                                              >  2:518492/1‑150 (MQ=255)                                                              ggggTGGCGACTAATACCGCATACCTCGACGGACTGATGAAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAACAAGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGTAGCGATGTGCCATACAAAGTGGCCa                                                                             <  2:355817/151‑1 (MQ=255)                                                                   ggCGACTAATACCGCATACCTCGACGGACTGATGAAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAACAAGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGTAGCGATGTGCCATACAAAGTGGCCATTaa                                                                         <  2:332528/150‑1 (MQ=255)                                                                    gCGACTAATACCGCATACCTCGACGGACTGATGAAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAACAAGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGTAGCGATGTGCCATACAAAGTGGCCATTAACg                                                                       <  2:612340/151‑1 (MQ=255)                                                                      gACTAATACCGCATACCTCGACGGACTGATGAAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAACAAGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGTAGCGATGTGCCATACAAAGTGGCCATTAACGa                                                                      <  2:35319/150‑1 (MQ=255)                                                                         tAATACCGCAAACCCCGACGGACTGATGAAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAACAAGCAGTACTGCTCATTGCGCTGTACGAACTGTCTAAACGTAGCGATGTGCCATACAAAGTGGCCATTAACGaa                                                                     <  1:610706/148‑1 (MQ=255)                                                                          aaTACCGCATACCTCGACGGACTGATGAAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAACAAGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGTAGCGATGTGCCATACAAAGTGGCCATTAACGAAGCg                                                                  >  1:802430/1‑150 (MQ=255)                                                                             aCCGCATACCTCGACGGACTGATGAAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAACAAGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGTAGCGATGTGCCATACAAAGTGGCCATTAACGAAGCGATCg                                                              >  2:819961/1‑151 (MQ=255)                                                                             aCCGCATACCTCGACGGACTGATGAAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAACAAGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGTAGCGATGTGCCATACAAAGTGGCCATTAACGAAGCGATCg                                                              >  2:391305/1‑151 (MQ=255)                                                                             aCCGCATACCTCGACGGACTGATGAAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAACAAGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGTAGCGATGTGCCATACAAAGTGGCCATTAACGAAGCGATCg                                                              >  1:306/1‑151 (MQ=255)                                                                              ccGCATCCCTCGACGGACTGAGGAAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAACAAGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGTAGCGATGTGCCATACAAAGTGGCCATTAACGAAGCGATCg                                                              <  2:398881/150‑1 (MQ=255)                                                                                 cATACCTCGACGGACTGATGAAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAACAAGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGTAGCGATGTGCCATACAAAGTGGCCATTAACGAAGCGATCGAACt                                                          >  1:523315/1‑151 (MQ=255)                                                                                  aTACCTCGACGGACTGATGAAGCCATCCCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAACAAGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGTAGCGATGTGCCATACAAAGTGGCCATTAACGAAGCGATCGAACTg                                                         <  1:321340/151‑1 (MQ=255)                                                                                   tACCTCGACGGACTGATGAAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAACAAGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGTAGCGATGTGCCATACAAAGTGGCCATTAACGAAGCGATCGAACTgg                                                        >  2:239109/1‑151 (MQ=255)                                                                                     ccTCGACGGACTGATGAAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAACAAGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGTAGCGATGTGCCATACAAAGTGGCCATTAACGAAGCGATCGAACTGGCg                                                      <  2:302979/151‑1 (MQ=255)                                                                                            ggACTGATGAAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAACAAGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGTAGCGATGTGCCATACAAAGTGGCCATTAACGAAGCGATCGAACTGGCGAAATCGt                                               >  2:807649/1‑151 (MQ=255)                                                                                                 gatgaGGCCATGCCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAACAAGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGTAGCGATGTGCCATACAAAGTGGCCATTAACGAAGCGATCGAACTGGCGAAATCGTTCGgc                                          <  2:911955/151‑1 (MQ=255)                                                                                                       gCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAACAAGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGTAGCGATGTGCCATACAAAGTGGCCATTAACGAAGCGATCGAACTGGAGAAATCGTTCGGCGCa                                       >  2:47735/1‑148 (MQ=255)                                                                                                                   ccGCCTGCTGGAAGAACTGGGACAGGTAGAACAAGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGTAGCGATGTGCCATACAAAGTGGCCATTAACGAAGCGATCGAACTGGCGAAATCGTTCGGCGCAGAAGACAGCCATa                          >  1:547560/1‑149 (MQ=255)                                                                                                                           tgGAAGAACTGGGACAGGTAGAACAAGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGTAGCGATGTGCCATACAAAGTGGCCATTAACGAAGCGATCGAACTGGCGAAATCGTTCGGCGCAGAAGACAGCCATAAGTTCGTCa                 >  2:26162/1‑150 (MQ=255)                                                                                                                            ggAAGAACTGGGACAGGTAGAACAAGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGTAGCGATGTGCCATACAAAGTGGCCATTAACGAAGCGATCGAACTGGCGAAATCGTTCGGCGCAGAAGACAGCCATAAGTTCGt                   <  1:219204/147‑1 (MQ=255)                                                                                                                            ggAAGAACTGGGACAGGTAGAACAAGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGTAGCGATGTGCCATACAAAGTGGCCATTAACGAAGCGATCGAACTGGCGAAATCGTTCGGCGCAGAAGACAGCCATAAGTTCGTCAAc               <  1:902764/151‑1 (MQ=255)                                                                                                                                 aaCTGGGACAGGTAGAACAAGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGTAGCGATGTGCCATACAAAGTGGCCATTAACGAAGCGATCGAACTGGCGAAATCGTTCGGCGCAGAAGACAGCCATAAGTTCGTCAACGGCGt          <  1:284056/151‑1 (MQ=255)                                                                                                                                   cTGGGACAGGTAGAACAAGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGTAGCGATGTGCCATACAAAGTGGCCATTAACGAAGCGATCGAACTGGCGAAATCGTTCGGCGCAGAAGACAGCCATAAGTTCGTCAACGGCGTAc        >  2:432030/1‑151 (MQ=255)                                                                                                                                       gACAGGTAGAACAAGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGTAGCGATGTGCCATACAAAGTGGCCATTAACGAAGCGATCGAACTGGCGAAATCGTTCGGCGCAGAAGACAGCCATAAGTTCGTCAACGGCGTACTCGa    <  2:148023/151‑1 (MQ=255)                                                                                                                                        aCAGGTAGAACAAGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGTAGCGATGTGCCATACAAAGTGGCCATTAACGAAGCGATCGAACTGGCGAAATCGTTCGGCGCAGAAGACAGCCATAAGTTCGTCAACGGCGTACTCGAt   >  1:218431/1‑151 (MQ=255)                                                                                                                                        aCAGGTAGAACAAGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGTAGCGATGTGCCATACAAAGTGGCCATTAACGAAGCGATCGAACTGGCGAAATCGTTCGGCGCAGAAGACAGCCATAAGTTCGTCAACGGCGTACTCGAt   <  1:171617/151‑1 (MQ=255)                                                                                                                                        aCAGGTAGAACAAGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGTAGCGATGTGCCATACAAAGTGGCCATTAACGAAGCGATCGAACTGGCGAAATCGTTCGGCGCAGAAGACAGCCATAAGTTCGTCAACGGCGTACTCGAt   <  1:713653/151‑1 (MQ=255)                                                                                                                                         cAGGTAGAACAAGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGTAGCGATGTGCCATACAAAGTGGCCATTAACGAAGCGATCGAACTGGCGAAATCGTTCGGCGCAGAAGACAGCCATAAGTTCGTCAACGGCGTACTCGATa  <  2:282718/151‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                              GTTCCTGGCTGAACAGGATGTAAAAGACGTTGACGTCCTGTACTTCCGTGAGCTGCTGGCCGGGGTGGCGACTAATACCGCATACCTCGACGGACTGATGAAGCCATACCTGTCCCGCCTGCTGGAAGAACTGGGACAGGTAGAAAAAGCAGTACTGCGCATTGCGCTGTACGAACTGTCTAAACGTAGCGATGTGCCATACAAAGTGGCCATTAACGAAGCGATCGAACTGGCGAAATCGTTCGGCGCAGAAGACAGCCATAAGTTCGTCAACGGCGTACTCGATA  >  NC_000913/435235‑435521

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑