Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	487,353	T→G	V273G (GTC→GGC)	mscK →	mechanosensitive channel protein, intermediate conductance, K+ regulated

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	487,353	0	T	G	100.0%	283.9 / NA	79	V273G (GTC→GGC)	mscK	mechanosensitive channel protein, intermediate conductance, K+ regulated
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (37/42);  total (37/42)

AACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAAGCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCGTCTGATTACCGCGACTGAAAACGGTAATCAGTTGATGCAGCAAAACATTAAAGTCAAAAACTGGC  >  NC_000913/487208‑487499                                                                                                                                                  |                                                                                                                                                   aaCACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAAGCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGGCTccc                                                                                                                                               >  1:235902/1‑151 (MQ=255)  acacCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAAGCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGGCTcccc                                                                                                                                              >  2:843823/1‑151 (MQ=255)  acacCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCA‑CAGTTACAACTGTTGCAAGAAGCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGGCTCCCCg                                                                                                                                             <  1:728830/151‑1 (MQ=255)      cGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAAGCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGGCTCCCCGGAt                                                                                                                                          <  1:748942/151‑1 (MQ=255)      cGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAAGCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGGCTCCCCGGAt                                                                                                                                          <  2:476726/151‑1 (MQ=255)           tGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAAGCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGGCTCCCCGGATGAAg                                                                                                                                      <  1:920451/150‑1 (MQ=255)            gCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAAGCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGGCTCCCCGGATGAAGc                                                                                                                                     <  2:250862/150‑1 (MQ=255)             cAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAAGCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGGCTCCCCGGATGAAGCcg                                                                                                                                   >  2:254790/1‑151 (MQ=255)              aGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAAGCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGGCTCCCCGGATGAAGCcgc                                                                                                                                  <  1:113393/151‑1 (MQ=255)               ggATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAAGCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGGCTCCCCGGATGAAGCcgcg                                                                                                                                 <  1:665570/151‑1 (MQ=255)                   aCCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAAGCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGGCTCCCCGGATGAAGCCGCGCGt                                                                                                                              >  2:5252/1‑150 (MQ=255)                       tGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAAGCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGGCTCCCCGGATGAAGCCGCGCGTAtt                                                                                                                           <  1:547182/149‑1 (MQ=255)                       tGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAAGCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGGCTCCCCGGATGAAGCCGCGCGTAtt                                                                                                                           <  2:903028/149‑1 (MQ=255)                         cAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAAGCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGGCTCCCCGGATGAAGCCGCGCGTATTCAgg                                                                                                                       >  2:626725/1‑151 (MQ=255)                              gCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAAGCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGGCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAAt                                                                                                                  >  2:271190/1‑151 (MQ=255)                                aaCGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAAGCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGGCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATcc                                                                                                                <  2:370508/151‑1 (MQ=255)                                aaCGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAAGCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGGCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATcc                                                                                                                <  2:2668/151‑1 (MQ=255)                                aaCGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAAGCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGGCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATcc                                                                                                                >  1:785626/1‑151 (MQ=255)                                    tGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAAGCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGGCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTg                                                                                                            >  1:731148/1‑151 (MQ=255)                                      aTTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAAGCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGGCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGt                                                                                                          >  2:459834/1‑151 (MQ=255)                                      aTTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAAGCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGGCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGt                                                                                                          <  2:323096/151‑1 (MQ=255)                                       ttACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAAGCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGGCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTg                                                                                                         >  1:40402/1‑151 (MQ=255)                                        tACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAAGCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGGCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTg                                                                                                         <  2:208182/150‑1 (MQ=255)                                         aCGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAAGCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGGCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGa                                                                                                        <  2:183962/150‑1 (MQ=255)                                          cGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAAGCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGGCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGaa                                                                                                       <  2:616743/150‑1 (MQ=255)                                          cGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAAGCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGGCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGa                                                                                                        >  1:90158/1‑149 (MQ=255)                                             gACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAAGCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGGCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCa                                                                                                    <  1:104426/150‑1 (MQ=255)                                              aCGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAAGCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGGCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAgg                                                                                                  >  1:481173/1‑151 (MQ=255)                                                ggCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAAGCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGGCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGaa                                                                                                <  1:516514/151‑1 (MQ=255)                                                   gAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAAGCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGGCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTg                                                                                             >  2:313546/1‑151 (MQ=255)                                                     aCAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAAGCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGGCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGa                                                                                           <  1:165449/151‑1 (MQ=255)                                                          gcTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAAGCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGGCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAAtt                                                                                       >  2:460192/1‑150 (MQ=255)                                                              gTCTGGAGCACCAGTTACAACTGTTGCAAGAAGCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGGCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAAcc                                                                                   >  2:777844/1‑150 (MQ=255)                                                              gTCTGGAGCACCAGTTACAACTGTTGCAAGAAGCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGGCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACca                                                                                  <  2:148362/151‑1 (MQ=255)                                                               tCTGGAGCACCAGTTACAACTGTTGCAAGAAGCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGGCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAAcc                                                                                   >  1:248602/1‑149 (MQ=255)                                                                   gAGCACCAGTTACAACTGTTGCAAGAAGCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGGCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGt                                                                             <  2:667353/151‑1 (MQ=255)                                                                    aGCACCAGTTACAACTGTTGCAAGAAGCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGGCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGtt                                                                            <  2:48209/151‑1 (MQ=255)                                                                         cAGTTACAACTGTTGCAAGAAGCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGGCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTc                                                                       >  2:890189/1‑151 (MQ=255)                                                                             tACAACTGTTGCAAGAAGCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGGCTACCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGc                                                                    <  2:540041/150‑1 (MQ=255)                                                                              aCAACTGTTGCAAGAAGCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGGCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCg                                                                   <  1:393534/150‑1 (MQ=255)                                                                              aCAACTGTTGCAAGAAGCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGGCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCGt                                                                  >  2:182171/1‑151 (MQ=255)                                                                                aaCTGTTGCAAGAAGCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGGCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCGTCt                                                                >  2:357929/1‑151 (MQ=255)                                                                                    gTTGCAAGAAGCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGGCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCGTCTGa                                                              <  1:351930/149‑1 (MQ=255)                                                                                      tGCAAGAAGCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGGCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCGTCTGATTAc                                                          >  1:386009/1‑151 (MQ=255)                                                                                        cAAGAAGCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGGCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCGTCTGATTACcg                                                        <  1:388620/151‑1 (MQ=255)                                                                                        cAAGAAGCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGGCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCGTCTGATTACcg                                                        <  1:245374/151‑1 (MQ=255)                                                                                        cAAGAAGCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGGCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCGTCTGATTACcg                                                        >  2:245024/1‑151 (MQ=255)                                                                                           gaagCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGGCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCGTCTGATTACcgcg                                                      >  2:229782/1‑150 (MQ=255)                                                                                                  tAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGGCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCGTCTGATTACCGCGACTGaaa                                               >  1:520314/1‑150 (MQ=255)                                                                                                   aaaCAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGGCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCGTCTGATTACCGCGACTGAAAAc                                             >  1:19967/1‑151 (MQ=255)                                                                                                    aaCAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGGCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCGTCTGATTACCGCGACTGAAAACg                                            >  1:257571/1‑151 (MQ=255)                                                                                                      cAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGGCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCGTCTGATTACCGCGACTGAAAACGGt                                          <  2:228242/151‑1 (MQ=255)                                                                                                        gCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGGCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCGTCTGATTACCGCGACTGAAAACGGTaa                                        <  2:205708/151‑1 (MQ=255)                                                                                                         cAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGGCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCGTCTGATTACCGCGACTGAAGACGGTAAt                                       <  2:936353/151‑1 (MQ=255)                                                                                                           aGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGGCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCGTCTGATTACCGCGACTGAAAACGGTAATCa                                     <  1:730168/151‑1 (MQ=255)                                                                                                            gcgcCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGGCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCGTCTGATTACCGCGACTGAAAACGGTAATCAg                                    <  1:87978/151‑1 (MQ=255)                                                                                                               ccTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGGCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCGTCTGATTACCGCGACTGAAAACGGTAATCAGTTg                                 >  1:462326/1‑151 (MQ=255)                                                                                                               ccTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGGCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCGTCTGATTACCGCGACTGAAAACGGTAATCAGTTg                                 <  1:928055/151‑1 (MQ=255)                                                                                                                     tttAACCGAAAAAACGGCGCAGGAAGCCGGCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCGTCTGATTACCGCGACTGAAAACGGTAATCAGTTGATgc                             >  2:550159/1‑149 (MQ=255)                                                                                                                       tAACCGAAAAAACGGCGCAGGAAGCCGGCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCGTCTGATTACCGCGACTGAAAACGGTAATCAGTTGATgcagc                          >  2:448795/1‑150 (MQ=255)                                                                                                                       tAACCGAAAAAACGGCGCAGGAAGCCGGCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCGTCTGATTACCGCGACTGAAAACGGTAATCAGTTGATgcagc                          >  1:398083/1‑150 (MQ=255)                                                                                                                        aaCCGAAAAAACGGCGCAGGAAGCCGGCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCGTCTGATTACCGCGACTGAAAACGGTAATCAGTTGATgcagca                         <  2:842385/150‑1 (MQ=255)                                                                                                                        aaCCGAAAAAACGGCGCAGGAAGCCGGCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCGTCTGATTACCGCGACTGAAAACGGTAATCAGTTGATgcagca                         >  1:250605/1‑150 (MQ=255)                                                                                                                             aaaaaaCGGCGCAGGAAGCCGGCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCGTCTGATTACCGCGACTGAAAACGGTAATCAGTTGATGCAGCAAAACAt                   >  1:657365/1‑151 (MQ=255)                                                                                                                              aaaaaCGGCGCAGGAAGCCGGCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCGTCTGATTACCGCGACTGAAAACGGTAATCAGTTGATGCAGCAAAACAtt                  <  1:170572/151‑1 (MQ=255)                                                                                                                              aaaaaCGGCGCAGGAAGCCGGCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCGTCTGATTACCGCGACTGAAAACGGTAATCAGTTGATGCAGCAAAACAtt                  >  2:858178/1‑151 (MQ=255)                                                                                                                               aaaaCGGCGCAGGAAGCCGGCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCGTCTGATTACCGCGACTGAAAACGGTAATCAGTTGATGCAGCAAAACATTa                 <  1:864494/151‑1 (MQ=255)                                                                                                                                 aaCGGCGCAGGAAGCCGGCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCGTCTGATTACCGCGACTGAAAACGGTAATCAGTTGATGCAGCAAAACAtt                  >  1:796310/1‑148 (MQ=255)                                                                                                                                    ggCGCAGGAAGCCGGCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCGTCTGATTACCGCGACTGAAAACGGTAATCAGTTGATGCAGCAAAACATTAAAGTc            <  2:298165/151‑1 (MQ=255)                                                                                                                                          ggAAGCCGGCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCGTCTGATTACCGCGACTGAAAACGGTAATCAGTTGATGCAGCAAAACATTAAAGTCAAAAAc      >  1:524402/1‑151 (MQ=255)                                                                                                                                           gAAGCCGGCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCGTCTGATTACCGCGACTGAAAACGGTAATCAGTTGATGCAGCAAAACATTAAAGTCAAAAAct     <  1:72843/151‑1 (MQ=255)                                                                                                                                           gAAGCCGGCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCGTCTGATTACCGCGACTGAAAACGGTAATCAGTTGATGCAGCAAAACATTAAAGTCAAAAAct     <  1:189476/151‑1 (MQ=255)                                                                                                                                            aaGCCGGCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCGTCTGATTACCGCGACTGAAAACGGTAATCAGTTGATGCAGCAAAACATTAAAGTCAAAAActg    >  1:527029/1‑151 (MQ=255)                                                                                                                                            aaGCCGGCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCGTCTGATTACCGCGACTGAAAACGGTAATCAGTTGATGCAGCAAAACATTAAAGTCAAAAActg    >  1:364674/1‑151 (MQ=255)                                                                                                                                            aaGCCGGCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCGTCTGATTACCGCGACTGAAAACGGTAATCAGTTGATGCAGCAAAACATTAAAGTCAAAAActg    <  1:182167/151‑1 (MQ=255)                                                                                                                                             aGCCGGCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCGTCTGATTACCGCGACTGAAAACGGTAATCAGTTGATGCAGCAAAACATTAAAGTCAAAAActgg   <  1:201308/151‑1 (MQ=255)                                                                                                                                             aGCCGGCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCGTCTGATTACCGCGACTGAAAACGGTAATCAGTTGATGCAGCAAAACATTAAAGTCAAAAActgg   <  1:175706/151‑1 (MQ=255)                                                                                                                                             aGCCGGCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCAGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCGTCTGATTACCGCGACTGAAAACGGTAATCAGTTGATGCAGCAAAACATTAAAGTCAAAAActgg   >  1:547717/1‑151 (MQ=255)                                                                                                                                              gCCGGCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCGTCTGATTACCGCGACTGAAAACGGTAATCAGTTGATGCAGCAAAACATTAAAGTCAAAAActggc  <  1:459819/151‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                                   AACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAAGCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCGTCTGATTACCGCGACTGAAAACGGTAATCAGTTGATGCAGCAAAACATTAAAGTCAAAAACTGGC  >  NC_000913/487208‑487499

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑