Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	729,193	T→G	V20V (GTT→GTG)	ybfA →	DUF2517 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	729,193	0	T	G	100.0%	289.9 / NA	78	V20V (GTT→GTG)	ybfA	DUF2517 family protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (45/33);  total (45/33)

ACACAAAAGGCGGTACTATTTTCATCAGATAAACAAATTCATTTTTCCGGTGCCATTCACCGGGTAGATACAAAAGGGGGAGAAAAGTATGGAACTCTACAGAGAATATCCTGCATGGCTTATCTTTTTACGCCGTACTTATGCGGTTGCAGCGGGCGTTCTGGCGCTGCCTTTCATGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGA  >  NC_000913/729046‑729340                                                                                                                                                    |                                                                                                                                                    acacAAAAGGCGGTACTATTTTCATCAGATAAACAAATTCATTTTTCCGGTGCCATTCACCGGGTAGATACAAAAGGGGGAGAAAAGTATGGAACTCTACAGAGAATATCCTGCATGGCTTATCTTTTTACGCCGTACTTATGCGGTGGc                                                                                                                                                   <  1:781730/150‑1 (MQ=255) acacAAAAGGCGGTACTATTTTCATCAGATAAACAAATTCATTTTTCCGGTGCCATTCACCGGGTAGATACAAAAGGGGGAGAAAAGTATGGAACTCTACAGAGAATATCCTGCATGGCTTATCTTTTTACGCCGTACTTATGCGGTGGCa                                                                                                                                                  <  1:784576/151‑1 (MQ=255)    caAAAGGCGGTACTATTTTCATCAGATAAACAAATTCATTTTTCCGGTGCCATTCACCGGGTAGATACAAAAGGGGGAGAAAAGTATGGAACTCTACAGAGAATATCCTGCATGGCTTATCTTTTTACGCCGTACTTATGCGGTGGCAGCg                                                                                                                                               >  1:194762/1‑151 (MQ=255)      aaaGGCGGTACTATTTTCATCAGATAAACAAATTCATTTTTCCGGTGCCATTCACCGGGTAGATACAAAAGGGGGAGAAAAGTATGGAACTCTACAGAGAATATCCTGCATGGCTTATCTTTTTACGCCGTACTTATGCGGTGGCAGCggg                                                                                                                                             <  1:79154/151‑1 (MQ=255)      aaaGGCGGTACTATTTTCATCAGATAAACAAATTCATTTTTCCGGTGCCATTCACCGGGTAGATACAAAAGGGGGAGAAAAGTATGGAACTCTACAGAGAATATCCTGCATGGCTTATCTTTTTACGCCGTACTTATGCGGAGGCAGCggg                                                                                                                                             <  1:52275/151‑1 (MQ=255)       aaGGCGGTACTATTTTCATCAGATAAACAAATTCATTTTTCCGGTGCCATTCACCGGGTAGATACAAAAGGGGGAGAAAAGTATGGAACTCTACAGAGAATATCCTGCATGGCTTATCTTTTTACGCCGTACTTATGCGGTGGCAGCGGGc                                                                                                                                            >  2:739862/1‑151 (MQ=255)        aGGCGGTACTATTTTCATCAGATAAACAAATTCATTTTTCCGGTGCCATTCACCGGGTAGATACAAAAGGGGGAGAAAAGTATGGAACTCTACAGAGAATATCCTGCATGGCTTATCTTTTTACGCCGTACTTATGCGGTGGCAGCGGGCg                                                                                                                                           <  2:548333/151‑1 (MQ=255)          gCGGTACTATTTTCATCAGATAAACAAATTCATTTTTCCGGTGCCATTCACCGGGTAGATACAAAAGGGGGAGAAAAGTATGGAACTCTACAGAGAATATCCTGCATGGCTTATCTTTTTACGCCGTACTTATGCGGTGGCAGCGGGCGtt                                                                                                                                         >  2:358968/1‑151 (MQ=255)           cGGTACTATTTTCATTAGATAAACAAATTCATTTTTCCGGTGCCATTCACCGGGTAGATACAAAAGGGGGAGAAAAGTATGGAACTCTACAGAGAATATCCTGCATGGCTTATCTTTTTACGCCGTACTTATGCGGTGGCAGCGGGCGTTc                                                                                                                                        >  1:580394/1‑151 (MQ=255)           cGGTACTATTTTCATCAGATAAACAAATTCATTTTTCCGGTGCCATTCACCGGGTAGATACAAAAGGGGGAGAAAAGTATGGAACTCTACAGAGAATATCCTGCATGGCTTATCTTTTTACGCCGTACTTATGCGGTGGCAGCGGGCGTTc                                                                                                                                        >  1:18065/1‑151 (MQ=255)           cGGTACTATTTTCATCAGATAAACAAATTCATTTTTCCGGTGCCATTCACCGGGTAGATACAAAAGGGGGAGAAAAGTATGGAACTCTACAGAGAATATCCTGCATGGCTTATCTTTTTACGCCGTACTTATGCGGTGGCAGCGGGCGTTc                                                                                                                                        >  2:615516/1‑151 (MQ=255)             gTACTATTTTCATCAGATAAACAAATTCATTTTTCCGGTGCCATTCACCGGGTAGATACAAAAGGGGGAGAAAAGTATGGAACTCTACAGAGAATATCCTGCATGGCTTATCTTTTTACGCCGTACTTATGCGGTGGCAGCGGGCGTTCTg                                                                                                                                      <  1:548037/151‑1 (MQ=255)               aCTATTTTCATCAGATAAACAAATTCATTTTTCCGGTGCCATTCACCGGGTAGATACAAAAGGGGGAGAAAAGTATGGAACTCTACAGAGAATATCCTGCATGGCTTATCTTTTTACGCCGTACTTATGCGGTGGCAGCGGGCGTTCTgg                                                                                                                                     <  2:198462/150‑1 (MQ=255)               aCTATTTTCATCAGATAAACAAATTCATTTTTCCGGTGCCATTCACCGGGTAGATACAAAAGGGGGAGAAAAGTATGGAACTCTACAGAGAATATCCTGCATGGCTTATCTTTTTACGCCGTACTTATGCGGTGGCAGCGGGCGTTCTGgc                                                                                                                                    >  2:590522/1‑151 (MQ=255)                 tATTTTCATCAGATAAACAAATTCATTTTTCCGGTGCCATTCACCGGGTAGATACAAAAGGGGGAGAAAAGTATGGAACTCTACAGAGAATATCCTGCATGGCTTATCTTTTTACGCCGTACTTATGCGGTGGCAGCGGGCGTTCTGgcgc                                                                                                                                  >  1:308267/1‑151 (MQ=255)                 tATTTTCATCAGATAAACAAATTCATTTTTCCGGTGCCATTCACCGGGTAGATACAAAAGGGGGAGAAAAGTATGGAACTCTACAGAGAATATCCTGCATGGCTTATCTTTTTACGCCGTACTTATGCGGTGGCAGCGGGCGTTCTGgcgc                                                                                                                                  >  1:48688/1‑151 (MQ=255)                  aTTTTCATCAGATAAACAAATTCATTTTTCCGGTGCCATTCACCGGGTAGATACAAAAGGGGGAGAAAAGTATGGAACTCTACAGAGAATATCCTGCATGGCTTATCTTTTTACGCCGTACTTATGCGGTGGCAGCGGGCGTTCTGGCGCt                                                                                                                                 <  2:132019/151‑1 (MQ=255)                  aTTTTCATCAGATAAACAAATTCATTTTTCCGGTGCCATTCACCGGGTAGATACAAAAGGGGGAGAAAAGTATGGAACTCTACAGAGAATATCCTGCATGGCTTATCTTTTTACGCCGTACTTATGCGGTGGCAGCGGGCGTTCTGGCGCt                                                                                                                                 >  2:655280/1‑151 (MQ=255)                    tttCATCAGATAAACAAATTCATTTTTCCGGTGCCATTCACCGGGAAGATACAAAAGGGGGAGAAAAGTATGGAACTCTACAGAGAATATCCTGCATGGCTTATCTTTTTACGCCGTACTTATGCGGTGGCAGCGGGCGTTCTGGCGCTGc                                                                                                                               >  2:786742/1‑151 (MQ=255)                        atcaGATAAACAAATTCATTTTTCCGGTGCCATTCACCGGGTAGATACAAAAGGGGGAGAAAAGTATGGAACTCTACAGAGAATATCCTGCATGGCTTATCTTTTTACGCCGTACTTATGCGGTGGCAGCGGGCGTTCTGGCGCTGCCttt                                                                                                                           >  1:192506/1‑151 (MQ=255)                          caGATAAACAAATTCATTTTTCCGGTGCCATTCACCGGGTAGATACAAAAGGGGGAGAAAAGTATGGAACTCTACAGAGAATATCCTGCATGGCTTATCTTTTTACGCCGTACTTATGCGGTGGCAGCGGGCGTTCTGGCGCTGCCTTTc                                                                                                                          >  1:259477/1‑150 (MQ=255)                          caGATAAACAAATTCATTTTTCCGGTGCCATTCACCGGGTAGATACAAAAGGGGGAGAAAAGTATGGAACTCTACAGAGAATATCCTGCATGGCTTATCTTTTTACGCCGTACTTATGCGGTGGCAGCGGGCGTTCTGGCGCTGCCTTTc                                                                                                                          >  1:665742/1‑150 (MQ=255)                               aaaCAAATTCATTTTTCCGGTGCCATTCACCGGGTAGATACAAAAGGGGGAGAAAAGTATGGAACTCTACAGAGAATATCCTGCATGGCTTATCTTTTTACGCCGTACTTATGCGGTGGCAGCGGGCGTTCTGGCGCTGCCTTTCATGCtt                                                                                                                    >  1:610615/1‑151 (MQ=255)                               aaaCAAATTCATTTTTCCGGTGCCATTCACCGGGTAGATACAAAAGGGGGAGAAAAGTATGGAACTCTACAGAGAATATCCTGCATGGCTTATCTTTTTACGCCGTACTTATGCGGTGGCAGCGGGCGTTCTGGCGCTGCCTTTCATGCt                                                                                                                     <  2:175660/150‑1 (MQ=255)                                aaCAAATTCATTTTTCCGGTGCCATTCACCGGGTAGATACAAAAGGGGGAGAAAAGTATGGAACTCTACAGAGAATATCCTGCATGGCTTATCTTTTTACGCCGTACTTATGCGGTGGCAGCGGGCGTTCTGGCGCTGCCTTTCATGCttt                                                                                                                   <  1:48178/151‑1 (MQ=255)                                 aCAAATTCATTTTTCCGGTGCCATTCACCGGGTAGATACAAAAGGGGGAGAAAAGTATGGAACTCTACAGAGAATATCCTGCATGGCTTATCTTTTTACGCCGTACTTATGCGGTGGCAGCGGGCGTTCTGGCGCTGCCTTTCATGCtttt                                                                                                                  >  2:345623/1‑151 (MQ=255)                                   aaaTTCATTTTTCCGGTGCCATTCACCGGGTAGATACAAAAGGGGGAGAAAAGTATGGAACTCTACAGAGAATATCCTGCATGGCTTATCTTTTTACGCCGTACTTATGCGGTGGCAGCGGGCGTTCTGGCGCTGCCTTTCATGCTTTTCt                                                                                                                >  1:302547/1‑151 (MQ=255)                                     aTTCATTTTTCCGGTGCCATTCACCGGGTAGATACAAAAGGGGGAGAAAAGTATGGAACTCTACAGAGAATATCCTGCATGGCTTATCTTTTTACGCCGTACTTATGTGGTGGCAGCGGGCGTTCTGGCGCTGCCTTTCATGCTTTTCTgg                                                                                                              >  1:9953/1‑151 (MQ=255)                                     aTTCATTTTTCCGGTGCCATTCACCGGGTAGATACAAAAGGGGGAGAAAAGTATGGAACTCTACAGAGAATATCCTGCATGGCTTATCTTTTTACGCCGTACTTATGCGGTGGCAGCGGGCGTTCTGGCGCTGCCTTTCATGCTTTTCTgg                                                                                                              <  1:841964/151‑1 (MQ=255)                                       tCATTTTTCCGGTGCCATTCACCGGGTAGATACAAAAGGGGGAGAAAAGTATGGAACTCTACAGAGAATATCCTGCATGGCTTATCTTTTTACGCCGTACTTATGCGGTGGCAGCGGGCGTTCTGGCGCTGCCTTTCATGCTTTTCTGGa                                                                                                             >  1:221825/1‑150 (MQ=255)                                         aTTTTTCCGGTGCCATTCACCGGGTAGATACAAAAGGGGGAGAAAAGTATGGAACTCTACAGAGAATATCCTGCATGGCTTATCTTTTTACGCCGTACTTATGCGGTGGCAGCGGGCGTTCTGGCGCTGCCTTTCATGCTTTTCTGGaa                                                                                                            >  1:35186/1‑149 (MQ=255)                                          tttttCCGGTGCCATTCACCGGGTAGATACAAAAGGGGGAGAAAAGTATGGAACTCTACAGAGAATATCCTGCATGGCTTATCTTTTTACGCCGTACTTATGCGGTGGCAGCGGGCGTTCTGGCGCTGCCTTTCATGCTTTTCTGGaa                                                                                                            >  2:929966/1‑148 (MQ=255)                                           ttttCCGGTGCCATTCACCGGGTAGATACAAAAGGGGGAGAAAAGTATGGAACTCTACAGAGAATATCCTGCATGGCTTATCTTTTTACGCCGTACTTATGCGGTGGCAGCGGGCGTTCTGGCGCTGCCTTTCATGCTTTTCTGGAAAGAc                                                                                                        >  2:10308/1‑151 (MQ=255)                                            tttCCGGTGCCATTCACCGGGTAGATACAAAAGGGGGAGAAAAGTATGGAACTCTACAGAGAATATCCTGCATGGCTTATCTTTTTACGCCGTACTTATGCGGTGGCAGCGGGCGTTCTGGCGCTGCCTTTCATGCTTTTCTGGAAAGAcc                                                                                                       >  1:727591/1‑151 (MQ=255)                                            tttCCGGTGCCATTCACCGGGTAGATACAAAAGGGGGAGAAAAGTATGGAACTCTACAGAGAATATCCTGCATGGCTTATCTTTTTACGCCGTACTTATGCGGTGGCAGCGGGCGTTCTGGCGCTGCCTTTCATGCTTTTCTGGAAAGAc                                                                                                        <  2:85057/150‑1 (MQ=255)                                              tCCGGTGCCATTCACCGGGTAGATACAAAAGGGGGAGAAAAGTATGGAACTCTACAGAGAATATCCTGCATGGCTTATCTTTTTACGCCGTACTTATGCGGTGGCAGCGGGCGTTCTGGCGCTGCCTTTCATGCTTTTCTGGAAAGACcgc                                                                                                     >  2:766632/1‑151 (MQ=255)                                               ccGGTGCCATTCACCGGGTAGATACAAAAGGGGGAGAAAAGTATGGAACTCTACAGAGAATATCCTGCATGGCTTATCTTTTTACGCCGTACTTATGCGGTGGCAGCGGGCGTTCTGGCGCTGCCTTTCATGCTTTTCTGGAAAGACcgcg                                                                                                    <  1:873821/151‑1 (MQ=255)                                               ccGGTGCCATTCACCGGGTAGATACAAAAGGGGGAGAAAAGTATGGAACTCTACAGAGAATATCCTGCATGGCTTATCTTTTTACGCCGTACTTATGCGGTGGCAGCGGGCGTTCTGGCGCTGCCTTTCATGCTTTTCTGGAAAGACcgc                                                                                                     <  1:79566/150‑1 (MQ=255)                                                cGGTGCCATTCACCGGGTAGATACAAAAGGGGGAGAAAAGTATGGAACTCTACAGAGAATATCCTGCATGGCTTATCTTTTTACGCCGTACTTATGCGGTGGCAGCGGGCGTTCTGGCGCTGCCTTTCATGCTTTTCTGGAAAGACCgcgc                                                                                                   >  2:850818/1‑151 (MQ=255)                                                 ggTGCCATTCACCGGGTAGATACAAAAGGGGGAGAAAAGTATGGAACTCTACAGAGAATATCCTGCATGGCTTATCTTTTTACGCCGTACTTATGCGGTGGCAGCGGGCGTTCTGGCGCTGCCTTTCATGCTTTTCTGGAAAGACCGCGcc                                                                                                  >  2:926242/1‑151 (MQ=255)                                                     ccATTCACCGGGTAGATACAAAAGGGGGAGAAAAGTATGGAACTCTACAGAGAATATCCTGCATGGCTTATCTTTTTACGCCGTACTTATGCGGTGGCAGCGGGCGTTCTGGCGCTGCCTTTCATGCTTTTCTGGAAAGACCGCGCCCGCt                                                                                              <  1:714520/151‑1 (MQ=255)                                                      cATTCACCGGGTAGATACAAAAGGGGGAGAAAAGTATGGAACTCTACAGAGAATATCCTGCATGGCTTATCTTTTTACGCCGTACTTATGCGGTGGCAGCGGGCGTTCTGGCGCTGCCTTTCATGCTTTTCTGGAAAGACCGCGCCCGCtt                                                                                             <  2:356038/151‑1 (MQ=255)                                                       aTTCACCGGGTAGATACAAAAGGGGGAGAAAAGTATGGAACTCTACAGAGAATATCCTGCATGGCTTATCTTTTTACGCCGTACTTATGCGGTGGCAGCGGGCGTTCTGGCGCTGCCTTTCATGCTTTTCTGGAAAGACCGCGCCCGCttt                                                                                            <  1:256185/151‑1 (MQ=255)                                                        ttCACCGGGTAGATACAAAAGGGGGAGAAAAGTATGGAACTCTACAGAGAATATCCTGCATGGCTTATCTTTTTACGCCGTACTTATGCGGTGGCAGCGGGCGTTCTGGCGCTGCCTTTCATGCTTTTCTGGAAAGACCGCGCCCGCtttt                                                                                           >  2:405681/1‑151 (MQ=255)                                                             cGGGTAGATACAAAAGGGGGAGAAAAGTATGGAACTCTACAGAGAATATCCTGCATGGCTTATCTTTTTACGCCGTACTTATGCGGTGGCAGCGGGCGTTCTGGCGCTGCCTTTCATGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGc                                                                                      >  1:894896/1‑151 (MQ=255)                                                                       cAAAAGGGGGAGAAAAGTATGGAACTCTACAGAGAATATCCTGCATGGCTTATCTTTTTACGCCGTACTTATGCGGTGGCAGCGGGCGTTCTGGCGCTGCCTTTCATGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATc                                                                            <  2:263649/151‑1 (MQ=255)                                                                         aaaGGGGGAGAAAAGTATGGAACTCTACAGAGAATATCCTGCATGGCTTATCTTTTTACGCCGTACTTATGCGGTGGCAGCGGGCGTTCTGGCGCTGCCTTTCATGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATcgc                                                                          <  1:132112/151‑1 (MQ=255)                                                                              gggAGAAAAGTATGGAACTCTACAGAGAATATCCTGCATGGCTTATCTTTTTACGCCGTACTTATGCGGTGGCAGCGGGCGTTCTGGCGCTGCCTTTCATGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTg                                                                     <  1:428186/151‑1 (MQ=255)                                                                              gggAGAAAAGTATGGAACTCTACAGAGAATATCCTGCATGGCTTATCTTTTTACGCCGTACTTATGCGGTGGCAGCGGGCGTTCTGGCGCTGCCTTTCATGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTg                                                                     <  2:640439/151‑1 (MQ=255)                                                                                 agaAAAGTATGGAACTCTACAGAGAATATCCTGCATGGCTTATCTTTTTACGCCGTACTTATGCGGTGGCAGCGGGCGTTCTGGCGCTGCCTTTCATGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTc                                                                  <  2:481677/151‑1 (MQ=255)                                                                                    aaaGTATGGAACTCTACAGAGAATATCCTGCATGGCTTATCTTTTTACGCCGTACTTATGCGGTGGCAGCGGGCGTTCTGGCGCTGCCTTTCATGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGa                                                                >  2:240009/1‑150 (MQ=255)                                                                                          tGGAACTCTACAGAGAATATCCTGCATGGCTTATCTTTTTACGCCGTACTTATGCGGTGGCAGCGGGCGTTCTGGCGCTGCCTTTCATGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAAcc                                                           >  2:379192/1‑149 (MQ=255)                                                                                          tGGAACTCTACAGAGAATATCCTGCATGGCTTATCTTTTTACGCCGTACTTATGCGGTGGCAGCGGGCGTTCTGGCGCTGCCTTTCATGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAAcc                                                           >  1:772536/1‑149 (MQ=255)                                                                                             aaCTCTACAGAGAATATCCTGCATGGCTTATCTTTTTACGCCGTACTTATGCGGTGGCAGCGGGCGTTCTGGCGCTGCCTTTCATGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCg                                                       >  2:474328/1‑150 (MQ=255)                                                                                               ctctACAGAGAATATCCTGCATGGCTTATCTTTTTACGCCGTACTTATGCGGTGGCAGCGGGCGTTCTGGCGCTGCCTTTCATGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGAt                                                     >  2:299539/1‑150 (MQ=255)                                                                                                tctACAGAGAATATCCTGCATGGCTTATCTTTTTACGCCGTACTTATGCGGTGGCAGCGGGCGTTCTGGCGCTGCCTTTCATGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATa                                                    >  2:106865/1‑150 (MQ=255)                                                                                                tctACAGAGAATATCCTGCATGGCTTATCTTTTTACGCCGTACTTATGCGGTGGCAGCGGGCGTTCTGGCGCTGCCTTTCATGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATa                                                    >  1:51669/1‑150 (MQ=255)                                                                                                 ctACAGAGAATATCCTGCATGGCTTATCTTTTTACGCCGTACTTATGCGGTGGCAGCGGGCGTTCTGGCGCTGCCTTTCATGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATaa                                                   >  2:584340/1‑150 (MQ=255)                                                                                                 ctACAGAGAATATCCTGCATGGCTTATCTTTTTACGCCGTACTTATGCGGTGGCAGCGGGCGTTCTGGCGCTGCCTTTCATGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATaa                                                   >  2:832421/1‑150 (MQ=255)                                                                                                   aCAGAGAATATCCTGCATGGCTTATCTTTTTACGCCGTACTTATGCGGTGGCAGCGGGCGTTCTGGCGCTGCCTTTCATGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAAcc                                                >  2:358510/1‑151 (MQ=255)                                                                                                   aCAGAGAATATCCTGCATGGCTTATCTTTTTACGCCGTACTTATGCGGTGGCAGCGGGCGTTCTGGCGCTGCCTTTCATGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAAcc                                                <  1:615496/151‑1 (MQ=255)                                                                                                   aCAGAGAATATCCTGCATGGCTTATCTTTTTACGCCGTACTTATGCGGTGGCAGCGGGCGTTCTGGCGCTGCCTTTCATGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAAcc                                                >  2:679780/1‑151 (MQ=255)                                                                                                            atCCTGCATGGCTTATCTTTTTACGCCGTACTTATGCGGTGGCAGCGGGCGTTCTGGCGCTGCCTTTCATGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGtggat                                       <  2:853646/151‑1 (MQ=255)                                                                                                                 gCATGGCTTATCTTTTTACGCCGTACTTATGCGGTGGCAGCGGGCGTTCTGGCGCTGCCTTTCATGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTggatggat                                   <  2:115910/150‑1 (MQ=255)                                                                                                                  cATGGCTTATCTTTTTACGCCGTACTTATGCGGTGGCAGCGGGCGTTCTGGCGCTGCCTTTCATGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATc                                  >  1:749432/1‑150 (MQ=255)                                                                                                                    tGGCTTATCTTTTTACGCCGTACTTATGCGGTGGCAGCGGGCGTTCTGGCGCTGCCTTTCATGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAgg                               >  1:765050/1‑151 (MQ=255)                                                                                                                        ttATCTTTTTACGCCGTACTTATGCGGTGGCAGCGGGCGTTCTGGCGCTGCCTTTCATGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCAGTGTGGATGGATCAGGCCg                            >  1:448419/1‑150 (MQ=255)                                                                                                                         tATCTTTTTACGCCGTACTTATGCGGTGGCAGCGGGCGTTCTGGCGCTGCCTTTCATGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCg                            <  1:368858/149‑1 (MQ=255)                                                                                                                          aTCTTTTTACGCCGTACTTATGCGGTGGCAGCGGGCGTTCTGGCGCTGCCTTTCATGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGaa                          <  2:762620/150‑1 (MQ=255)                                                                                                                          aTCTTTTTACGCCGTACTTATGCGGTGGCAGCGGGCGTTCTGGCGCTGCCTTTCATGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGaa                          <  1:379178/150‑1 (MQ=255)                                                                                                                              ttttACGCCGTACTTATGCGGTGGCAGCGGGCGTTCTGGCGCTGCCTTTCATGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGaaaa                        <  2:203685/148‑1 (MQ=255)                                                                                                                                ttACGCCGTACTTATGCGGTGGCAGCGGGCGTTCTGGCGCTGCCTTTCATGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCa                    <  2:518496/150‑1 (MQ=255)                                                                                                                                      cGTACTTATGCGGTGGCAGCGGGCGTTCTGGCGCTGCCTTTCATGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTg             >  2:364095/1‑151 (MQ=255)                                                                                                                                         aCTTATGCGGTGGCAGCGGGCGTTCTGGCGCTGCCTTTCATGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGAt           <  2:95342/150‑1 (MQ=255)                                                                                                                                          cTTATGCGGTGGCAGCGGGCGTTCTGGCGCTGCCTTTCATGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGAttt         >  2:417876/1‑151 (MQ=255)                                                                                                                                            tATGCGGTGGCAGCGGGCGTTCTGGCGCTGCCTTTCATGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGAtttt        <  2:877588/150‑1 (MQ=255)                                                                                                                                              tGCGGTGGCAGCGGGCGCTCTGGCGCTGCCTTTCATGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTat     >  1:684858/1‑151 (MQ=255)                                                                                                                                                 ggTGGCAGCGGGCGTTCTGGCGCTGCCTTTCATGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGTAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTattga  <  2:808861/151‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                    ACACAAAAGGCGGTACTATTTTCATCAGATAAACAAATTCATTTTTCCGGTGCCATTCACCGGGTAGATACAAAAGGGGGAGAAAAGTATGGAACTCTACAGAGAATATCCTGCATGGCTTATCTTTTTACGCCGTACTTATGCGGTTGCAGCGGGCGTTCTGGCGCTGCCTTTCATGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGA  >  NC_000913/729046‑729340

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑