Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	46,879	A→C	Y358S (TAT→TCT)	yaaU →	putative MFS sugar transporter; membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	46,879	0	A	C	100.0%	130.4 / NA	38	Y358S (TAT→TCT)	yaaU	putative MFS sugar transporter; membrane protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (17/21);  total (17/21)

CCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGGTTTTGGGGCTAATCCCGGATATGGGGATCTGGCTGGTAGTGATGGCCTTTGCGGTGTATGCCTTTTTCTCTGGCGGGCCGGGTAATTTGCAGTGGCTCTATCCTAATGAACTCTTCCCGACAGATATCCGCGCCTCTGCCGTGGGCGTGATTATGTCCTTAAGTCGTATTGGCACCATTGTTTCGACCTGGGCACTACCGATCTTTATCAATAATTACGGTATCAGTAACACGATGC  >  NC_000913/46734‑47016                                                                                                                                                  |                                                                                                                                          ccATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGGTTTTGGGGCTAATCCCGGATATGGGGATCTGGCTGGTAGTGATGGCCTTTGCGGGGTATGCCTTTTTCTCTGGCGGGCCGGGGAATTTGCAGTGGCTCTCTcc                                                                                                                                        >  1:554239/1‑149 (MQ=255)      gttgATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGGTTTTGGGGCTAATCCCGGATATGGGGATCTGGCTGGTAGTGATGGCCTTTGCGGTGTATGCCTTTTTCTCTGGCGGGCCGGGTAATTTGCAGTGGCTCTCTCCTAATGa                                                                                                                                  <  2:881548/150‑1 (MQ=255)        tgATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGGTTTTGGGGCTAATCCCGGATATGGGGATCTGGCTGGTAGTGATGGCCTTTGCGGTGTATGCCTTTTTCTCTGGCGGGCCGGGTAATTTGCAGTGGCTCTCTCCTAATGAAc                                                                                                                                <  1:818157/150‑1 (MQ=255)          aTTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGGTTTTGGGGCTAATCCCGGATATGGGGATCTGGCTGGTAGTGATGGCCTTTGCGGTGTATGCCTTTTTCTCTGGCGGGCCGGGTAATTTGCAGTGGCTCTCTCCTAATGAActct                                                                                                                             <  2:723361/151‑1 (MQ=255)            tGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGGTTTTGGGGCTAATGCCGGATATTGGGATCTGGCTGGGAGTGATGGGCTTTGCGGCGGATGCCTTTTTCTCTGGCGGGCCGGGGAATTTGCAGTGGCTCTCTCCTAATTAACTCtt                                                                                                                            >  1:78067/1‑150 (MQ=255)             ggCAGCTTTGCCATGATGACGCTGGCGCTGGCGGTTTTGGGGCTAATCCCGGATATGGGGATCTGGCTGGTAGTGATGGCCTTTGCGGTGTATGCCTTTTTCTCTGGCGGGCCGGGTAATTTGCAGTGGCTCTCTCCTAATGAACTCTTcc                                                                                                                          <  2:842004/151‑1 (MQ=255)               cAGCTTTGCCATGATGACGCTGGCGCTGGCGGTTTTGGGGCTAATCCCGGATATGGGGATCTGGCTGGTAGTGATGGCCTTTGCGGGGTATGCCTTTTTCTCTGGCGGGCCGGGTAATTTGCAGTGGCTCTCTCCTAATGAACTCTTccc                                                                                                                         >  1:834432/1‑150 (MQ=255)                     tGCCATGATGACGCTGGCGCTGGCGGTTTTGGGGCTAATCCCGGATATGGGGATCTGGCTGGTAGTGATGGCCTTTGCGGTGTATGCCTTTTTCTCTGGCGGGCCGGGTAATTTGCAGTGGCTCTCTCCTAATGAACTCTTCCCggc                                                                                                                      >  2:500884/1‑145 (MQ=255)                         atgatgACGCTGGCGCTGGCGGTTTTGGGGCTAATCCCGGATATGGGGATCTGGCTGGTAGTGATGGCCTTTGCGGTGTATGCCTTTTTCTCTGGCGGGCCGGGTAATTTGCAGTGGCTCTCTCCTAATGAACTCTTCCCGACAGATATcc                                                                                                              <  1:93037/151‑1 (MQ=255)                          tgatgaCGCTGGCGCTGGCGGTTTTGGGGCTAATCCCGGATATGGGGATCTGGCTGGTAGTGATGGCCTTTGCGGTGTATGCCTTTTTCTCTGGCGGGCCGGGTAATTTGCAGTGGCTCTCTCCTAATGAACTCTTCCCGACAGATATCcg                                                                                                             >  2:176436/1‑151 (MQ=255)                                 gctggcgctggcGGTTTTGGGGCTAATCCCGGATATGGGGATCTGGCTGGTAGTGATGGCCTTTGCGGTGTATGCCTTTTTCTCTGGCGGGCCGGGTAATTTGCAGTGGCTCTCTCCTAATGAACTCTTCCCGACAGATATCCGCGCctct                                                                                                      <  2:242613/151‑1 (MQ=255)                                      cgctggcgGTTTTGGGGCTAATCCCGGATATGGGGATCTGGCTGGTAGTGATGGCCTTTGCGGTGTATGCCTTTTTCTCTGGCGGGCCGGGTAATTTGCAGTGGCTCTCTCCTAATGAACTCTTCCCGACAGATATCCGCGCCTCTGCCGt                                                                                                 <  2:761403/151‑1 (MQ=255)                                          ggcgGTTTTGGGGCTAATCCCGGATATGGGGATCTGGCTGGTAGTGATGGCCTTTGCGGTGTATGCCTTTTTCTCTGGCGGGCCGGGTAATTTGCAGTGGCTCTCTCCTAATGAACTCTTCCCGACAGATATCCGCGCCTCTGCCGTgg                                                                                               <  2:831034/149‑1 (MQ=255)                                          ggcgGTTTTGGGGCTAATCCCGGATATGGGGATCTGGCTGGTAGTGATGGCCTTTGCGGTGTATGCCTTTTTCTCTGGCGGGCCGGGTAATTTGCAGTGGCTCTCTCCTAATGAACTCTTCCCGACAGATATCCGCGCCTCTGCCGTGGGc                                                                                             <  1:512381/151‑1 (MQ=255)                                              gTTTTGGGGCTAATCCCGGATATGGGGATCTGGCTGGTAGTGATGGCCTTTGCGGTGTATGCCTTTTTCTCTGGCGGGCCGGGTAATTTGCAGTGGCTCTCTCCTAATGAACTCTTCCCGACAGATATCCGCGCCTCTGCCGTGGGCGTg                                                                                          >  2:470848/1‑150 (MQ=255)                                              gTTTTGGGGCTAATCCCGGATATGGGGATCTGGCTGGTAGTGATGGCCTTTGCGGTGTATGCCTTTTTCTCTGGCGGGCCGGGTAATTTGCAGTGACTCTCTCCTAATGAACTCTTCCCGACAGATATCCGCGCCTCTGCCGTGGGCGTg                                                                                          >  1:556832/1‑150 (MQ=255)                                                  tGGGGCTAATCCCGGATATGGGGATCTGGCTGGTAGTGATGGCCTTTGCGGTGTATGCCTTTTTCTCTGGCGGGCCGGGTAATTTGCAGTGGCTCTCTCCTAATGAACTCTTCCCGACAGATATCCGCGCCTCTGCCGTGGGCGTGATTAt                                                                                     >  2:230017/1‑151 (MQ=255)                                                    gggctatggcaggATAGGGGGATCTGGCTGGTAGTGATGGCCTTTGCGGTGTATGCATTTTCCTCTGGCGGGCCGGGTAATTTGCAGTGGCTGTCACCGAATGATCTCTTCCCGACAGATATCCGCGCCTCTGCCGTGGGCGTGATTa                                                                                      <  1:55623/137‑1 (MQ=255)                                                            cccGGCTATGGGGATCTGGCTGGTTGTGATGACCTTTGCGGTGTATGCCTTTTTATCTGGCGGGCCGGGAAATTTGCGGTGGCCCTCCCCTAATGAACTCTTACCAACAGATATCCGCGCCTCTGCCGTGGGCGGGATTATGTcc                                                                                 >  1:641686/1‑145 (MQ=255)                                                                     ggggATCTGGCTGGTAGTGATGGCCTTTGCGGTGTATGCCTTTTTCTCTGGCGGGCCGGGTAATTTGCAGTGGCTCTCTCCTAATGAACTCTTCCCGACAGATATCCGCGCCTCTGCCGTGGGCGTGATTATGTCCTTAAGTCGTATTgg                                                                   >  2:924572/1‑150 (MQ=255)                                                                       ggATCTGGCTGGTAGTGATGGCCTTTGCGGTGTATGCCTTTTTCTCTGGCGGGCCGGGTAATTTGCAGTGGCTCTCTCCTAATGAACTCTTCCCGACAGATATCCGCGCCTCTGCCGTGGGCGTGATTATGTCCTTAAGTCGTATTGGCAc                                                                <  1:399437/151‑1 (MQ=255)                                                                                tggTAGTGATGGCCTTTGCGGTGTATGCCTTTTTCTCTGGCGGGCCGGGTAATTTGCAGTGGCTCTCTCCTAATGAACTCTTCCCGACAGATATCCGCGCCTCTGCCGTGGGCGTGATTATGTCCTTAAGTCGTATTGGCACCATTGTTTc                                                       >  2:512095/1‑151 (MQ=255)                                                                                 ggTAGTGATGGCCTTTGCGGTGTATGCCTTTTTCTCTGGCGGGCCGGGTAATTTGCAGTGGCTCTCTCCTAATGAACTCTTCCCGACAGATATCCGCGCCTCTGCCGTGGGCGTGATTATGTCCTTAAGTCGTATTGGCACCATTGTTTCg                                                      <  2:212786/151‑1 (MQ=255)                                                                                    aGTGATGGCCTTTGCGGTGTATGCCTTTTTCTCTGGCGGGCCGGGTAATTTGCAGTGGCTCTCTCCTAATGAACTCTTCCCGACAGATATCCGCGCCTCTGCCGTGGGCGTGATTATGTCCTTAAGTCGTATTGGCACCATTGTTTCGAcc                                                   <  1:496190/151‑1 (MQ=255)                                                                                         tGGCCTTTGCGGTGTATGCCTTTTTCTCTGGCGGGCCGGGTAATTTGCAGTGGCTCTCTCCTAATGAACTCTTCCCGACAGATATCCGCGCCTCTGCCGTGGGCGTGATTATGTCCTTAAGTCGTATTGGCACCATTGTTTCGACCTGGGc                                              >  2:88268/1‑151 (MQ=255)                                                                                                     tgtATGCCTTTTTCTCTGGCGGGCCGGGTAATTTGCAGTGGCTCTCTCCTAATGAACTCTTCCCGACAGATATCCGCGCCTCTGCCGTGGGCGTGATTATGTCCTTAAGTCGTATTGGCACCATTGTTTCGACCTGGGCACTACCGATCtt                                  >  1:922694/1‑151 (MQ=255)                                                                                                      gtATGCCTTTTTCTCTGGCGGGCCGGGTAATTTGCAGTGGCTCTCTCCTAATGAACTCTTCCCGACAGATATCCGCGCCTCTGCCGTGGGCGTGATTATGTCCTTAAGTCGTATTGGCACCATTGTTTCGACCTGGGCACTACCGATCttt                                 <  2:126578/151‑1 (MQ=255)                                                                                                      gtATGCCTTTTTCTCTGGCGGGCCGGGTAATTTGCAGTGGCTCTCTCCTAATGAACTCTTCCCGACAGATATCCGCGCCTCTGCCGTGGGCGTGATTATGTCCTTAAGTCGTATTGGCACCATTGTTTCGACCTGGGCACTACCGATCtt                                  <  2:146815/150‑1 (MQ=255)                                                                                                           ccTTTTTCTCTGGCGGGCCGGGTAATTTGCAGTGGCTCTCTCCTAATGAACTCTTCCCGACAGATATCCGCGCCTCTGCCGTGGGCGTGATTATGTCCTTAAGTCGTATTGGCACCATTGTTTCGACCTGGGCACTACCGATCTTTATCaa                            <  1:65562/151‑1 (MQ=255)                                                                                                           ccTTTTTCTCTGGCGGGCCGGGTAATTTGCAGTGGCTCTCTCCTAATGAACTCTTCCCGACAGATATCCGCGCCTCTGCCGTGGGCGTGATTATGTCCTTAAGTCGTATTGGCACCATTGTTTCGACCTGGGCACTACCGATCTTTATCa                             <  2:283284/150‑1 (MQ=255)                                                                                                              ttttCTCTGGCGGGCCGGGTAATTTGCAGTGGCTCTCTCCTAATGAACTCTTCCCGACAGATATCCGCGCCTCTGCCGTGGGCGTGATTATGTCCTTAAGTCGTATTGGCACCATTGTTTCGACCTGGGCACTACCGATCTTTATCaat                           <  1:459755/149‑1 (MQ=255)                                                                                                               tttCTCTGGCGGGCCGGGTAATTTGCAGTGGCTCTCTCCTAATGAACTCTTCCCGACAGATATCCGCGCCTCTGCCGTGGGCGTGATTATGTCCTTAAGTCGTATTGGCACCATTGTTTCGACCTGGGCACTACCGATCTTTATCaataat                        >  2:614926/1‑151 (MQ=255)                                                                                                                 tctcTGGCGGGCCGGGCAATTTGCAGTGGCTCTCTCCTAATGAACTCTTCCCGACAGATATCCGCGCCTCTGCCGTGGGCGTGATTATGTCCTTAAGTCGTATTGGCACCATTGTTTCGACCTGGGCACTACCGATCTTTATCAATAAtt                       >  2:333935/1‑150 (MQ=255)                                                                                                                    ctGGCGGGCCGGGTAATTTGCAGTGGCTCTCTCCTAATGAACTCTTCCCGACAGATATCCGCGCCTCTGCCGTGGGCGTGATTATGTCCTTAAGTCGTATTGGCACCATTGTTTCGACCTGGGCACTACCGATCTTTATCAATAATTACgg                   >  1:645319/1‑151 (MQ=255)                                                                                                                           gCCGGGTAATTTGCAGTGGCTCTCTCCTAATGAACTCTTCCCGACAGATATCCGCGCCTCTGCCGTGGGCGTGATTATGTCCTTAAGTCGTATTGGCACCATTGTTTCGACCTGGGCACTACCGATCTTTATCAATAATTACGGTATCAGt            <  2:683650/151‑1 (MQ=255)                                                                                                                                  aaTTTGCAGTGGCTCTCTCCTAATGAACTCTTCCCGACAGATATCCGCGCCTCTGCCGTGGGCGTGATTATGTCCTTAAGTCGTATTGGCACCATTGTTTCGACCTGGGCACTACCGATCTTTATCAATAATTACGGTATCAGTAACACGa     <  2:891428/151‑1 (MQ=255)                                                                                                                                   aTTTGCAGTGGCTCTCTCCTAATGAACTCTTCCCGACAGATATCCGCGCATCTGCCGTGGGCGTGATTATGCCCTTAAGTCGTATTGGCACCATTGTTTCGACCTGGGCACTACCGATCTTTATCAATAATTACGGTATCAGTAACACg      >  1:776423/1‑149 (MQ=255)                                                                                                                                      tGCAGTGGCTCTCTCCTAATGAACTCTTCCCGACAGATATCCGCGCCTCTGCCGTGGGCGTGATTATGTCCTTAAGTCGTATTGGCACCATTGTTTCGACCTGGGCACTACCGATCTTTATCAATAATTACGGTATCAGTAACACGATGc  <  2:835179/150‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                          CCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGGTTTTGGGGCTAATCCCGGATATGGGGATCTGGCTGGTAGTGATGGCCTTTGCGGTGTATGCCTTTTTCTCTGGCGGGCCGGGTAATTTGCAGTGGCTCTATCCTAATGAACTCTTCCCGACAGATATCCGCGCCTCTGCCGTGGGCGTGATTATGTCCTTAAGTCGTATTGGCACCATTGTTTCGACCTGGGCACTACCGATCTTTATCAATAATTACGGTATCAGTAACACGATGC  >  NC_000913/46734‑47016

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑