Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	838,474	A→C	intergenic (‑18/+56)	ybiI ← / ← ybiX	DksA‑type zinc finger protein/Fe(II)‑dependent oxygenase superfamily protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	838,474	0	A	C	100.0%	212.5 / NA	55	intergenic (‑18/+56)	ybiI/ybiX	DksA‑type zinc finger protein/Fe(II)‑dependent oxygenase superfamily protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (23/32);  total (23/32)

GCACCGCACTCTTCACATTCATCCAGGCTTTCGCCGCGCGGAATTTCACCCCGAGCGCGGGCAATCGCATCTTCAATTGTACTGTTGATCTGTTCGTTGACGGCGTCATCGTTAGCCCAACCGGATGCCATATCGACCTCCCCATATCAATACTTGTACAGTTAAGTGTAGCTAATCCAGGGACGAACTCGGGCAGTTCAAGCATCAGATCTCCGACCATTCCCGCAGCAGATTATGATAAAGATTAAGCAGCGACAGGATCTCTTCACTTTCGCCGTAGCGGCTTTTCAGCGACT  >  NC_000913/838326‑838621                                                                                                                                                     |                                                                                                                                                    gCACCGCACTCTTCACATTCATCCAGGCTTTCGCCGCGCGGAATTTCACCCCGAGCGCGGGCAATCGCATCTTCAATTGTACTGTTGATCTGTTCGTTGACGGCGTCATCGTTAGCCCAACCGGATGCCATATCGACCTCCCCATATCCAt                                                                                                                                                   <  2:2662/151‑1 (MQ=255)  cACCGCACTCTTCACATTCATCCAGGCTTTCGCCGCGCGGAATTTCACCCCGAGCGCGGGCAATCGCATCTTCAATTGTACTGTTGATCTGTTCGTTGACGGCGTCATCGTTAGCCCAACCGGATGCCATATCGACCTCCCCATATCCAt                                                                                                                                                   >  2:591803/1‑150 (MQ=255)   aCCGCACTCTTCACATTCATCCAGGCTTTCGCCGCGCGGAATTTCACCCCGAGCGCGGGCAATCGCATCTTCAATTGTACTGTTGATCTGTTCGTTGACGGCGTCATCGTTAGCCCAACCGGATGCCATATCGACCTCCCCATATCCATAc                                                                                                                                                 <  1:124568/151‑1 (MQ=255)         ctctTCACATTCATCCAGGCTTTCGCCGCGCGGAATTTCACCCCGAGCGCGGGCAATCGCATCTTCAATTGTACTGTTGATCTGTTCGTTGACGGCGTCATCGTTAGCCCAACCGGATGCCATATCGACCTCCCCATATCCATACTTGTAc                                                                                                                                           >  2:930514/1‑151 (MQ=255)          tctTCACATTCATCCAGGCTTTCGCCGCGCGGAATTTCACCCCGAGCGCGGGCAATCGCATCTTCAATTGTACTGTTGATCTGTTCGTTGACGGCGTCATCGTTAGCCCAACCGGATGCCATATCGACCTCCCCATATCCATACTTGTAc                                                                                                                                           >  1:315257/1‑150 (MQ=255)             tCACATTCATCCAGGCTTTCGCCGCGCGGAATTTCACCCCGAGCGCGGGCAATCGCATCTTCAATTGTACTGTTGATCTGTTCGTTGACGGCGTCATCGTTAGCCCAACCGGATGCCATATCGACCTCCCCATATCCATACTTGTACa                                                                                                                                          <  2:224420/148‑1 (MQ=255)             tCACATTCATCCAGGCTTTCGCCGCGCGGAATTTCACCCCGAGCGCGGGCAATCGCATCTTCAATTGTACTGTTGATCTGTTCGTTGACGGCGTCATCGTTAGCCCAACCGGATGCCATATCGACCTCCCCATATCCATACTTGTACa                                                                                                                                          <  2:182464/148‑1 (MQ=255)                              ttCGCCGCGCGGAATTTCACCCCGAGCGCGGGCAATCGCATCTTCAATTGTACTGTTGATCTGTTCGTTGACGGCGTCATCGTTAGCCCAACCGGATGCCATATCGACCTCCCCATATCCATACTTGTACAGTTAAGTGTAGCTAATcc                                                                                                                        <  2:786333/149‑1 (MQ=255)                              ttCGCCGCGCGGAATTTCACCCCGAGCGCGGGCAATCGCATCTTCAATTGTACTGTTGATCTGTTCGTTGACGGCGTCATCGTTAGCCCAACCGGATGCCATATCGACCTCCCCATATCCATACTTGTACAGTTAAGTGTAGCTAATcc                                                                                                                        <  1:366013/149‑1 (MQ=255)                               tCGCCGCGCGGAATTTCACCCCGAGCGCGGGCAATCGCATCTTCAATTGTACTGTTGATCTGTTCGTTGACGGCGTCATCGTTAGCCCAACCGGATGCCATATCGACCTCCCCATATCCATACTTGTACAGTTAAGTGTAGCTAATCCAg                                                                                                                      <  2:184005/150‑1 (MQ=255)                                cgccgcGCGGAATTTCACCCCGAGCGCGGGCAATCGCATCTTCAATTGTACTGTTGATCTGTTCGTTGACGGCGTCATCGTTAGCCCAACCGGATGCCATATCGACCTCCCCATATCCATACTTGTACAGTTAAGTGTAGCTAATCCAggg                                                                                                                    <  2:550493/151‑1 (MQ=255)                                cgccgcGCGGAATTTCACCCCGAGCGCGGGCAATCGCATCTTCAATTGTACTGTTGATCTGTTCGTTGACGGCGTCATCGTTAGCCCAACCGGATGCCATATCGACCTCCCCATATCCATACTTGTACAGTTAAGTGTAGCTAATCCAggg                                                                                                                    >  1:725568/1‑151 (MQ=255)                                  ccgcGCGGAATTTCACCCCGAGCGCGGGCAATCGCATCTTCAATTGTACTGTTGATCTGTTCGTTGACGGCGTCATCGTTAGCCCAACCGGATGCCATATCGACCTCCCCATATCCATACTTGTACAGTTAAGTGTAGCTAATCCAGGGAc                                                                                                                  <  2:696279/151‑1 (MQ=255)                                  ccgcGCGGAATTTCACCCCGAGCGCGGGCAATCGCATCTTCAATTGTACTGTTGATCTGTTCGTTGACGGCGTCATCGTTAGCCCAACCGGATGCCATATCGACCTCCCCATATCCATACTTGTACAGTTAAGTGTAGCTAATCCAGGGAc                                                                                                                  >  2:665085/1‑151 (MQ=255)                                  ccgcGCGGAATTTCACCCCGAGCGCGGGCAATCGCATCTTCAATTGTACTGTTGATCTGTTCGTTGACGGCGTCATCGTTAGCCCAACCGGATGCCATATCGACCTCCCCATATCCATACTTGTACAGTTAAGTGTAGCTAATCCAGGGAc                                                                                                                  <  1:789414/151‑1 (MQ=255)                                    gcgcgGAATTTCACCCCGAGCGCGGGCAATCGCATCTTCAATTGTACTGTTGATCTGTTCGTTGACGGCGTCATCGTTAGCCCAACCGGATGCCATATCGACCTCCCCATATCCATACTTGTACAGTTAAGTGTAGCTAATCCAGGGACg                                                                                                                 >  1:126355/1‑150 (MQ=255)                                    gcgcgGAATTTCACCCCGAGCGCGGGCAATCGCATCTTCAATTGTACTGTTGATCTGTTCGTTGACGGCGTCATCGTTAGCCCAACCGGATGCCATATCGACCTCCCCATATCCATACTTGTACAGTTAAGTGTAGCTAATCCAGGGACGa                                                                                                                <  2:637153/151‑1 (MQ=255)                                      gcgGAATTTCACCCCGAGCGCGGGCAATCGCATCTTCAATTGTACTGTTGATCTGTTCGTTGACGGCGTCATCGTTAGCCCAACCGGATGCCATATCGACCTCCCCATATCCATACTTGTACAGTTAAGTGTAGCTAATCCAGGGACGAAc                                                                                                              >  2:322721/1‑151 (MQ=255)                                                aCCCCGAGCGCGGGCAATCGCATCTTCAATTGTACTGTTGATCTGTTCGTTGACGGCGTCATCGTTAGCCCAACCGGATGCCATATCGACCTCCCCATATCCATACTTGTACAGTTAAGTGTAGCTAATCCAGGGACGAACTCGGGCAGtt                                                                                                    <  1:914577/151‑1 (MQ=255)                                                     gAGCGCGGGCAATCGCATCTTCAATTGTACTGTTGATCTGTTCGTTGACGGCGTCATCGTTAGCCCAACCGGATGCCATATCGACCTCCCCATATCCATACTTGTACAGTTAAGAGTAGCTAATCCAGGGACGAACTCGGGCAGTTCAAg                                                                                                >  1:353528/1‑150 (MQ=255)                                                         gcgGGCAATCGCATCTTCAATTGTACTGTTGATCTGTTCGTTGACGGCGTCATCGTTAGCCCAACCGGATGCCATATCGACCTCCCCATATCCATACTTGTACAGTTAAGTGTAGCTAATCCAGGGACGAACTCGGGCAGTTCAAGCATCa                                                                                           <  1:28941/151‑1 (MQ=255)                                                                aTCGCATCTTCAATTGTACTGTTGATCTGTTCGTTGACGGCGTCATCGTTAGCCCAACCGGATGCCATATCGACCTCCCCATATCCATACTTGTACAGTTAAGTGTAGCTAATCCAGGGACGAACTCGGGCAGTTCAAGCATCAGATCTcc                                                                                    <  2:182577/151‑1 (MQ=255)                                                                  cGCATCTTCAATTGTACTGTTGATCTGTTCGTTGACGGCGTCATCGTTAGCCCAACCGGATGCCATATCGACCTCCCCATATCCATACTTGTACAGTTAAGTGTAGCTAATCCAGGGACGAACTCGGGCAGTTCAAGCATCAGATCTCCg                                                                                   <  1:490957/150‑1 (MQ=255)                                                                         tCAATTGTACTGTTGATCTGTTCGTTGACGGCGTCATCGTTAGCCCAACCGGATGCCATATCGACCTCCCCATATCCATACTTGTACAGTTAAGTGTAGCTAATCCAGGGACGAACTCGGGCAGTTCAAGCATCAGATCTCCGACCAt                                                                              <  1:715109/148‑1 (MQ=255)                                                                          cAATTGTACTGTTGATCTGTTCGTTGACGGCGTCATCGTTAGCCCAACCGGATGCCATATCGACCTCCCCATATCCATACTTGTACAGTTAAGTGTAGCTAATCCAGGGACGAACTCGGGCAGTTCAAGCATCAGATCTCCGACCATTccc                                                                          <  2:465821/151‑1 (MQ=255)                                                                          cAATTGTACTGTTGATCTGTTCGTTGACGGCGTCATCGTTAGCCCAACCGGATGCCATATCGACCTCCCCATATCCATACTTGTACAGTTAAGTGTAGCTAATCCAGGGACGAACTCGGGCAGTTCAAGCATCAGATCTCCGACCATTcc                                                                           <  1:442710/150‑1 (MQ=255)                                                                             ttGTACTGTTGATCTGTTCGTTGACGGCGTCATCGTTAGCCCAACCGGATGCCATATCGACCTCCCCATATCCATACTTGTACAGTTAAGTGTAGCTAATCCAGGGACGAACTCGGGCAGTTCAAGCATCAGATCTCCGACCATTCCCgc                                                                        >  1:922642/1‑150 (MQ=255)                                                                               gTACTGTTGATCTGTTCGTTGACGGCGTCATCGTTAGCCCAACCGGATGCCATATCGACCTCCCCATATCCATACTTGTACAGTTAAGTGTAGCTAATCCAGGGACGAACTCGGGCAGTTCAAGCATCAGATCTCCGACCATTCCCgcagc                                                                     <  2:614989/151‑1 (MQ=255)                                                                                 aCTGTTGATCTGTTCGTTGACGGCGTCATCGTTAGCCCAACCGGATGCCATATCGACCTCCCCATATCCATACTTGTACAGTTAAGTGTAGCTAATCCAGGGACGAACTCGGGCAGTTCAAGCATCAGATCTCCGACCATTCCCgcagc                                                                     >  2:781104/1‑149 (MQ=255)                                                                                 aCTGTTGATCTGTTCGTTGACGGCGTCATCGTTAGCCCAACCGGATGCCATATCGACCTCCCCATATCCATACTTGTACAGTTAAGTGTAGCTAATCCAGGGACGAACTCGGGCAGTTCAAGCATCAGATCTCCGACCATTCCCGcagcag                                                                   <  2:16887/151‑1 (MQ=255)                                                                                 aCTGTTGATCTGTTCGTTGACGGCGTCATCGTTAGCCCAACCGGATGCCATATCGACCTCCCCATATCCATACTTGTACAGTTAAGTGTAGCTAATCCAGGGACGAACTCGGGCAGTTCAAGCATCAGATCTCCGACCATTCCCGcagcag                                                                   <  1:906814/151‑1 (MQ=255)                                                                                    gTTGATCTGTTCGTTGACGGCGTCATCGTTAGCCCAACCGGATGCCATATCGACCTCCCCATATCCATACTTGTACAGTTAAGTGTAGCTAATCCAGGGACGAACTCGGGCAGTTCAAGCATCAGATCTCCGACCATTCCCGCAGCAGa                                                                  <  1:929381/149‑1 (MQ=255)                                                                                    gTTGATCTGTTCGTTGACGGCGTCATCGTTAGCCCAACCGGATGCCATATCGACCTCCCCATATCCATACTTGTACAGTTAAGTGTAGCTAATCCAGGGACGAACTCGGGCAGTTCAAGCATCAGATCTCCGACCATTCCCGCAGCAGAt                                                                 <  2:407513/150‑1 (MQ=255)                                                                                      tGATCTGTTCGTTGACGGCGTCATCGTTAGCCCAACCGGATGCCATATCGACCTCCCCATATCCATACTTGTACAGTTAAGTGTAGCTAATCCAGGGACGAACTCGGGCAGTTCAAGCATCAGATCTCCGACCATTCCCGCAGCAGATTAt                                                              >  1:517134/1‑151 (MQ=255)                                                                                           tGTTCGTTGACGGCGTCATCGTTAGCCCAACCGGATGCCATATCGACCTCCCCATATCCATACTTGTACAGTTAAGTGTAGCTAATCCAGGGACGAACTCGGGCAGTTCAAGCATCAGATCTCCGACCATTCCCGCAGCAGATTATGATa                                                          >  1:18784/1‑150 (MQ=255)                                                                                              tCGTTGACGGCGTCATCGTTAGCCCAACCGGATGCCATATCGACCTCCCCATATCCATACTTGTACAGTTAAGTGTAGCTAATCCAGGGACGAACTCGGGCAGTTCAAGCATCAGATCTCCGACCATTCCCGCAGCAGATTATGATAAAg                                                       <  2:629911/150‑1 (MQ=255)                                                                                                gTTGACGGCGTCATCGTTAGCCCAACCGGATGCCATATCGACCTCCCCATATCCATACTTGTACAGTTAAGTGTAGCTAATCCAGGGACGAACTCGGGCAGTTCAAGCATCAGATCTCCGACCATTCCCGCAGCAGATTATGATAAAGAtt                                                    <  1:569529/151‑1 (MQ=255)                                                                                                gTTGACGGCGTCATCGTTAGCCCAACCGGATGCCATATCGACCTCCCCATATCCATACTTGTACAGTTAAGTGTAGCTAATCCAGGGACGAACTCGGGCAGTTCAAGCATCAGATCTCCGACCATTCCCGCAGCAGATTATGATAAAGAtt                                                    >  1:671989/1‑151 (MQ=255)                                                                                                   gACGGCGTCATCGTTAGCCCAACCGGATGCCATATCGACCTCCCCATATCCATACTTGTACAGTTAAGTGTAGCTAATCCAGGGACGAACTCGGGCAGTTCAAGCATCAGATCTCCGACCATTCCCGCAGCAGATTATGATAAAGATTaa                                                  <  1:591784/150‑1 (MQ=255)                                                                                                     cGGCGTCATCGTTAGCCCAACCGGATGCCATATCGACCTCCCCATATCCATACTTGTACAGTTAAGTGTAGCTAATCCAGGGACGAACTCGGGCAGTTCAAGCATCAGATCTCCGACCATTCCCGCAGCAGATTATGATAAAGATTAagc                                                >  2:674272/1‑150 (MQ=255)                                                                                                     cGGCGTCATCGTTAGCCCAACCGGATGCCATATCGACCTCCCCATATCCATACTTGTACAGTTAAGTGTAGCTAATCCAGGGACGAACTCGGGCAGTTCAAGCATCAGATCTCCGACCATTCCCGCAGCAGATTATGATAAAGATTAagc                                                >  2:225957/1‑150 (MQ=255)                                                                                                                  aGCCCAACCGGATGCCATATCGACCTCCCCATATCCATACTTGTACAGTTAAGTGTAGCTAATCCAGGGACGAACTCGGGCAGTTCAAGCATCAGATCTCCGACCATTCCCGCAGCAGATTATGATAAAGATTAAGCAGCGACAGGAtct                                   <  2:408972/150‑1 (MQ=255)                                                                                                                     ccAACCGGATGCCATATCGACCTCCCCATATCCATACTTGTACAGTTAAGTGTAGCTAATCCAGGGACGAACTCGGGCAGTTCAAGCATCAGATCTCCGACCATTCCCGCAGCAGATTATGATAAAGATTAAGCAGCGACAGGATCTCTTc                               <  2:357403/151‑1 (MQ=255)                                                                                                                          cGGATGCCATATCGACCTCCCCATATCCATACTTGTACAGTTAAGTGTAGCTAATCCAGGGACGAACTCGGGCAGTTCAAGCATCAGATCTCCGACCATTCCCGCAGCAGATTATGATAAAGATTAAGCAGCGACAGGATCTCTTCACttt                          <  2:910985/151‑1 (MQ=255)                                                                                                                           ggATGCCATATCGACCTCCCCATATCCATACTTGTACAGTTAAGTGTAGCTAATCCAGGGACGAACTCGGGCAGTTCAAGCATCAGATCTCCGACCATTCCCGCAGCAGATTATGATAAAGATTAAGCAGCGACAGGATCTCTTCACTTTc                         <  1:134026/151‑1 (MQ=255)                                                                                                                            gATGCCATATCGACCTCCCCATATCCATACTTGTACAGTTAAGTGTAGCTAATCCAGGGACGAACTCGGGCAGTTCAAGCATCAGATCTCCGACCATTCCCGCAGCAGATTATGATAAAGATTAAGCAGCGACAGGATCTCTTCACTTTCg                        <  1:607944/151‑1 (MQ=255)                                                                                                                              tGCCATATCGACCTCCCCATATCCATACTTGTACAGTTAAGTGTAGCTAATCCAGGGACGAACTCGGGCAGTTCAAGCATCAGATCTCCGACCATTCCCGCAGCAGATTATGATAAAGATTAAGCAGCGACAGGATCTCTTCACTTTCGcc                      >  2:26998/1‑151 (MQ=255)                                                                                                                               gCCATATCGACCTCCCCATATCCATACTTGTACAGTTAAGTGTAGCTAATCCAGGGACGAACTCGGGCAGTTCAAGCATCAGATCTCCGACCATTCCCGCAGCAGATTATGATAAAGATTAAGCAGCGACAGGATCTCTTCACTTTCGcc                      <  2:162588/150‑1 (MQ=255)                                                                                                                                      cGACCTCCCCATATCCATACTTGTACAGTTAAGTGTAGCTAATCCAGGGACGAACTCGGGCAGTTCAAGCATCAGATCTCCGACCATTCCCGCAGCAGATTATGATAAAGATTAAGCAGCGACAGGATCTCTTCACTTTCGCCGTAGCGGc              >  1:371596/1‑151 (MQ=255)                                                                                                                                       gACCTCCCCATATCCATACTTGTACAGTTAAGTGTAGCTAATCCAGGGACGAACTCGGGCAGTTCAAGCATCAGATCTCCGACCATTCCCGCAGCAGATTATGATAAAGATTAAGCAGCGACAGGATCTCTTCACTTTCGCCGTAGCGGCt             >  2:301010/1‑151 (MQ=255)                                                                                                                                           tCCCCATATCCATACTTGTACAGTTAAGTGTAGCTAATCCAGGGACGAACTCGGGCAGTTCAAGCATCAGATCTCCGACCATTCCCGCAGCAGATTATGATAAAGATTAAGCAGCGACAGGATCTCTTCACTTTCGCCGTAGCGGCTTTTc         >  2:664268/1‑151 (MQ=255)                                                                                                                                             cccATATCCATACTTGTACAGTTAAGTGTAGCTAATCCAGGGACGAACTCGGGCAGTTCAAGCATCAGATCTCCGACCATTCCCGCAGCAGATTATGATAAAGATTAAGCAGCGACAGGATCTCTTCACTTTCGCCGTAGCGGCTTTTc         >  1:711170/1‑149 (MQ=255)                                                                                                                                               cATATCCATACTTGTACAGTTAAGTGTAGCTAATCCAGGGACGAACTCGGGCAGTTCAAGCATCAGATCTCCGACCATTCCCGCAGCAGATTATGATAAAGATTAAGCAGCGACAGGATCTCTTCACTTTCGCCGTAGCGGCTTTTCAGCg     >  2:513185/1‑151 (MQ=255)                                                                                                                                                atatCCATACTTGTACAGTTAAGTGTAGCTAATCCAGGGACGAACTCGGGCAGTTCAAGCATCAGATCTCCGACCATTCCCGCAGCAGATTATGATAAAGATTAAGCAGCGACAGGATCTCTTCACTTTCGCCGTAGCGGCTTTTCAGCg     >  1:347374/1‑150 (MQ=255)                                                                                                                                                  atCCATACTTGTACAGTTAAGTGTAGCTAATCCAGGGACGAACTCGGGCAGTTCAAGCATCAGATCTCCGACCATTCCCGCAGCAGATTATGATAAAGATTAAGCAGCGACAGGATCTCTTCACTTTCGCCGTAGCGGCTTTTCAGCGACt  >  1:403935/1‑151 (MQ=255)                                                                                                                                                     |                                                                                                                                                    GCACCGCACTCTTCACATTCATCCAGGCTTTCGCCGCGCGGAATTTCACCCCGAGCGCGGGCAATCGCATCTTCAATTGTACTGTTGATCTGTTCGTTGACGGCGTCATCGTTAGCCCAACCGGATGCCATATCGACCTCCCCATATCAATACTTGTACAGTTAAGTGTAGCTAATCCAGGGACGAACTCGGGCAGTTCAAGCATCAGATCTCCGACCATTCCCGCAGCAGATTATGATAAAGATTAAGCAGCGACAGGATCTCTTCACTTTCGCCGTAGCGGCTTTTCAGCGACT  >  NC_000913/838326‑838621

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑