Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 936,254 A→C K1011Q (AAA→CAA)  ftsK → DNA translocase at septal ring sorting daughter chromsomes

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913936,2540AC100.0% 161.9 / NA 42K1011Q (AAA→CAA) ftsKDNA translocase at septal ring sorting daughter chromsomes
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (18/24);  total (18/24)

GTCGCCATTAACCGTGGTGCTGGGTAAAGATATCGCCGGTGAGCCGGTGGTTGCCGATCTGGCGAAAATGCCGCACTTGTTGGTTGCGGGGACTACCGGTTCCGGTAAATCTGTCGGTGTGAACGCGATGATCCTGAGCATGCTTTATAAAGCACAGCCAGAAGATGTGCGTTTCATCATGATCGACCCGAAAATGCTGGAGCTTTCGGTTTATGAAGGCATTCCGCATCTGTTAACGGAAGTCGTTACTGATATGAAAGATGCCGCCAACGCGCTGCGCTGGTGTGTTAAC  >  NC_000913/936106‑936397
                                                                                                                                                    |                                                                                                                                               
gTCGCCATTAACCGTGGTGCTGGGTAAAGATATCGCCGGTGAGCCGGTGGTTGCCGATCTGGCGAAAATGCCGCACTTGTTGGTTGCGGGGACTACCGGTTCCGGTAAATCTGTCGGTGTGAACGCGATGATCCTGAGCATGCTTTATcaa                                                                                                                                               <  2:3361/151‑1 (MQ=255)
  cGCCATTAACCGTGGTGCTGGGTAAAGATATCGCCGGTGAGCCGGTGGTTGCCGATCTGGCGAAAATGCCGCACTTGTTGGTTGCGGGGACTACCGGTTCCGGTAAATCTGTCGGTGTGAACGCGATGATCCTGAGCATGCTTTATCAAGc                                                                                                                                             <  2:494464/151‑1 (MQ=255)
    ccATTAACCGTGGTGCTGGGTAAAGATATCGCCGGTGAGCCGGTGGTTGCCGATCTGGCGAAAATGCCGCACTTGTTGGTTGCGGGGACTACCGGTTCCGGTAAATCTGTCGGTGTGAACGCGATGATCCTGAGCATGCTTTATCAAGcac                                                                                                                                           <  1:897116/151‑1 (MQ=255)
        tAACCGTGGTGCTGGGTAAAGATATCGCCGGTGAGCCGGTGGTTGCCGATCTGGCGAAAATGCCGCACTTGTTGGTTGCGGGGACTACCGGTTCCGGTAAATCTGTCGGTGTGAACGCGATGATCCTGAGCATGCTTTATCAAGCACAGc                                                                                                                                        <  2:166862/150‑1 (MQ=255)
        tAACCGTGGTGCTGGGTAAAGATATCGCCGGTGAGCCGGTGGTTGCCGATCTGGCGAAAATGCCGCACTTGTTGGTTGCGGGGACTACCGGTTCCGGTAAATCTGTCGGTGTGAACGCGATGATCCTGAGCATGCTTTATCAAGCACAGc                                                                                                                                        <  1:916867/150‑1 (MQ=255)
         aaCCGTGGTGCTGGGTAAAGATATCGCCGGTGAGCCGGTGGTTGCCGATCTGGCGAAAATGCCGCACTTGTTGGTTGCGGGGACTACCGGTTCCGGTAAATCTGTCGGTGTGAACGCGATGATCCTGAGCATGCTTTATCAAGCACAGcc                                                                                                                                       <  1:209896/150‑1 (MQ=255)
                             atatCGCCGGTGAGCCGGTGGTTGCCGATCTGGCGAAAATGCCGCACTTGTTGGTTGCGGGGACTACCGGTTCCGGTAAATCTGTCGGTGTGAACGCGATGATCCTGAGCATGCTTTATCAAGCACAGCCAGAAGATGTGCGTTTcatcat                                                                                                                  <  1:237658/151‑1 (MQ=255)
                               atCGCCGGTGAGCCGGTGGTTGCCGATCTGGCGAAAATGCCGCACTTGTTGGTTGCGGGGACTACCGGTTCCGGTAAATCTGTCGGTGTGAACGCGATGATCCTGAGCATGCTTTATCAAGCACAGCCAGAAGATGTGCGTTTCATCATGa                                                                                                                <  2:700026/151‑1 (MQ=255)
                                  gCCGGTGAGCCGGTGGTTGCCGATCTGGCGAAAATGCCGCACTTGTTGGTTGCGGGGACTACCGGTTCCGGTAAATCTGTCGGTGTGAACGCGATGATCCTGAGCATGCTTTATCAAGCACAGCCAGAAGATGTGCGTTTCATCATGATCg                                                                                                             >  2:904057/1‑151 (MQ=255)
                                    cGGTGAGCCGGTGGTTGCCGATCTGGCGAAAATGCCGCACTTGTTGGTTGCGGGGACTACCGGTTCCGGTAAATCTGTCGGTGTGAACGCGATGATCCTGAGCATGCTTTATCAAGCACAGCCAGAAGATGTGCGTTTCATCATGATCGAc                                                                                                           <  2:430435/151‑1 (MQ=255)
                                     ggTGAGCCGGTGGTTGCCGATCTGGCGAAAATGCCGCACTTGTTGGTTGCGGGGACTACCGGTTCCGGTAAATCTGTCGGTGTGAACGCGATGATCCTGAGCATGCTTTATCAAGCACAGCCAGAAGATGTGCGTTTCATCATGATCGAcc                                                                                                          <  2:658855/151‑1 (MQ=255)
                                     ggTGAGCCGGTGGTTGCCGATCTGGCGAAAATGCCGCACTTGTTGGTTGCGGGGACTACCGGTTCCGGTAAATCTGTCGGTGTGAACGCGATGATCCTGAGCATGCTTTATCAAGCACAGCCAGAAGATGTGCGTTTCATCATGATCGAcc                                                                                                          >  2:380446/1‑151 (MQ=255)
                                     ggTGAGCCGGTGGTTGCCGATCTGGCGAAAATGCCGCACTTGTTGGTTGCGGGGACTACCGGTTCCGGTAAATCTGTCGGTGTGAACGCGATGATCCTGAGCATGCTTTATCAAGCACAGCCAGAAGATGTGCGTTTCATCATGATCGAcc                                                                                                          <  2:263998/151‑1 (MQ=255)
                                           ccGGTGGTTGCCGATCTGGCGAAAATGCCGCACTTGTTGGTTGCGGGGACTACCGGTTCCGGTAAATCTGTCGGTGTGAACGCGATGATCCTGAGCATGCTTTATCAAGCACAGCCAGAAGATGTGCGTTTCATCATGATCGACCCGaaa                                                                                                     >  2:732708/1‑150 (MQ=255)
                                                   tGCCGATCTGGCGAAAATGCCGCACTTGTTGGTTGCGGGGACTACCGGTTCCGGTAAATCTGTCGGTGTGAACGCGATGATCCTGAGCATGCTTTATCAAGCACAGCCAGAAGATGTGCGTTTCATCATGATCGACCCGAAAATGCTgg                                                                                              >  2:170926/1‑149 (MQ=255)
                                                    gCCGATCTGGCGAAAATGCCGCACTTGTTGGTTGCGGGGACTACCGGTTCCGGTAAATCTGTCGGTGTGAACGCGATGATCCTGAGCATGCTTTATCAAGCACAGCCAGAAGATGTGCGTTTCATCATGATCGACCCGAAAATGCTGGa                                                                                             >  2:323015/1‑149 (MQ=255)
                                                    gCCGATCTGGCGAAAATGCCGCACTTGTTGGTTGCGGGGACTACCGGTTCCGGTAAATCTGTCGGTGTGAACGCGATGATCCTGAGCATGCTTTATCAAGCACAGCCAGAAGATGTGCGTTTCATCATGATCGACCCGAAAATGCTGGAg                                                                                            <  2:896340/150‑1 (MQ=255)
                                                    gCCGATCTGGCGAAAATGCCGCACTTGTTGGTTGCGGGGACTACCGGTTCCGGTAAATCTGTCGGTGTGAACGCGATGATCCTGAGCATGCTTTATCAAGCACAGCCAGAAGATGTGCGTTTCATCATGATCGACCCGAAAATGCTGGAGc                                                                                           <  2:701427/151‑1 (MQ=255)
                                                        aTCTGGCGAAAATGCCGCACTTGTTGGTTGCGGGGACTACCGGTTCCGGTAAATCTGTCGGTGTGAACGCGATGATCCTGAGCATGCTTTATCAAGCACAGCCAGAAGATGTGCGTTTCATCATGATCGACCCGAAAATGCTGGAGCTTTc                                                                                       >  2:648609/1‑151 (MQ=255)
                                                        aTCTGGCGAAAATGCCGCACTTGTTGGTTGCGGGGACTACCGGTTCCGGTAAATCTGTCGGTGTGAACGCGATGATCCTGAGCATGCTTTATCAAGCACAGCCAGAAGATGTGCGTTTCATCATGATCGACCCGAAAATGCTGGAGCTTTc                                                                                       >  1:606690/1‑151 (MQ=255)
                                                           tGGCGAAAATGCCGCACTTGTTGGTTGCGGGGACTACCGGTTCCGGTAAATCTGTCGGTGTGAACGCGATGATCCTGAGCATGCTTTATCAAGCACAGCCAGAAGATGTGCGTTTCATCATGATCGACCCGAAAATGCTGGAGCTTTCGGt                                                                                    >  1:413367/1‑151 (MQ=255)
                                                            ggCGAAAATGCCGCACTTGTTGGTTGCGGGGACTACCGGTTCCGGTAAATCTGTCGGTGTGAACGCGATGATCCTGAGCATGCTTTATCAAGCACAGCCAGAAGATGTGCGTTTCATCATGATCGACCCGAAAATGCTGGAGCttt                                                                                        <  1:729478/146‑1 (MQ=255)
                                                            ggCGAAAATGCCGCACTTGTTGGTTGCGGGGACTACCGGTTCCGGTAAATCTGTCGGTGTGAACGCGATGATCCTGAGCATGCTTTATCAAGCACAGCCAGAAGATGTGCGTTTCATCATGATCGACCCGAAAATGCTGGAGCTTTc                                                                                       <  2:279818/147‑1 (MQ=255)
                                                                             tgttgGTTGCGGGGACTACCGGTTCCGGTAAATCTGTCGGTGTGAACGCGATGATCCTGAGCATGCTTTATCAAGCACAGCCAGAAGATGTGCGTTTCATCATGATCGACCCGAAAATGCTGGAGCTTTCGGTTTATGAAGGCATTCCg                                                                    <  2:476965/149‑1 (MQ=255)
                                                                              gttggttgCGGGGACTACCGGTTCCGGTAAATCTGTCGGTGTGAACGCGATGATCCTGAGCATGCTTTATCAAGCACAGCCAGAAGATGTGCGTTTCATCATGATCGACCCGAAAATGCTGGAGCTTTCGGTTTATGAAGGCATTCCGCAt                                                                 <  1:505270/151‑1 (MQ=255)
                                                                                tggttgCGGGGACTACCGGTTCCGGTAAATCTGTCGGTGTGAACGCGATGATCCTGAGCATGCTTTATCAAGCACAGCCAGAAGATGTGCGTTTCATCATGATCGACCCGAAAATGCTGGAGCTTTCGGTTTATGAAGGCATTCCGCATc                                                                <  2:306788/150‑1 (MQ=255)
                                                                                             tACCGGTTCCGGTAAATCTGTCGGTGTGAACGCGATGATCCTGAGCATGCTTTATCAAGCACAGCCAGAAGATGTGCGTTTCATCATGATCGACCCGAAAATGCTGGAGCTTTCGGTTTATGAAGGCATTCCGCATCTGTTAACGGAAGTc                                                  >  1:25080/1‑151 (MQ=255)
                                                                                                     ccGGTAAATCTGTCGGTGTGAACGCGATGATCCTGAGCATGCTTTATCAAGCACAGCCAGAAGATGTGCGTTTCATCATGATCGACCCGAAAATGCTGGAGCTTTCGGTTTATGAAGGCATTCCGCATCTGTTAACGGAAGTCGTTACt                                            <  2:410803/149‑1 (MQ=255)
                                                                                                     ccGGTAAATCTGTCGGTGTGAACGCGATGATCCTGAGCATGCTTTATCAAGCACAGCCAGAAGATGTGCGTTTCATCATGATCGACCCGAAAATGCTGGAGCTTTCGGTTTATGAAGGCATTCCGCATCTGTTAACGGAAGTCGTTACTg                                           >  1:754968/1‑150 (MQ=255)
                                                                                                          aaaTCTGTCGGTGTGAACGCGATGATCCTGAGCATGCTTTATCAAGCACAGCCAGAAGATGTGCGTTTCATCATGATCGACCCGAAAATGCTGGAGCTTTCGGTTTATGAAGGCATTCCGCATCTGTTAACGGAAGTCGTTACTGATATg                                      >  1:430840/1‑150 (MQ=255)
                                                                                                           aaTCTGTCGGTGTGAACGCGATGATCCTGAGCATGCTTTATCAAGCACAGCCAGAAGATGTGCGTTTCATCATGATCGACCCGAAAATGCTGGAGCTTTCGGTTTATGAAGGCATTCCGCATCTGTTAACGGAAGTCGTTACTGATATGa                                     >  1:916605/1‑150 (MQ=255)
                                                                                                                gTCGGTGTGAACGCGATGATCCTGAGCATGCTTTATCAAGCACAGCCAGAGGATGTGCGTTTCATCATGATCGACCCGAAAATGCTGGAGCTTTCGGTTTATGAAGGCATTCCGCATCTGTTAACGGAAGTCGTTACTGATATGaaa                                   <  1:788888/147‑1 (MQ=255)
                                                                                                                   ggTGTGAACGCGATGATCCTGAGCATGCTTTATCAAGCACAGCCAGAAGATGTGCGTTTCATCATGATCGACCCGAAAATGCTGGAGCTTTCGGTTTATGAAGGCATTCCGCATCTGTTAACGGAAGTCGTTACTGATATGAAAGATgccg                            >  2:372189/1‑151 (MQ=255)
                                                                                                                   ggTGTGAACGCGATGATCCTGAGCATGCTTTATCAAGCACAGCCAGAAGATGTGCGTTTCATCATGATCGACCCGAAAATGCTGGAGCTTTCGGTTTATGAAGGCATTCCGCATCTGTTAACGGAAGTCGTTACTGATATGAAAGATgccg                            >  2:256548/1‑151 (MQ=255)
                                                                                                                     tgtgAACGCGATGATCCTGAGCATGCTTTATCAAGCACAGCCAGAAGATGTGCGTTTCATCATGATCGACCCGAAAATGCTGGAGCTTTCGGTTTATGAAGGCATTCCGCATCTGTTAACGGAAGTCGTTACTGATATGAAAGATgccgcc                          >  1:770326/1‑151 (MQ=255)
                                                                                                                        gAACGCGATGATCCTGAGCATGCTTTATCAAGCACAGCCAGAAGATGTGCGTTTCATCATGATCGACCCGAAAATGCTGGAGCTTTCGGTTTATGAAGGCATTCCGCATCTGTTAACGGAAGTCGTTACTGATATGAAAGATGCCGCCaa                        <  2:778915/150‑1 (MQ=255)
                                                                                                                             cgATGATCCTGAGCATGCTTTATCAAGCACAGCCAGAAGATGTGCGTTTCATCATGATCGACCCGAAAATGCTGGAGCTTTCGGTTTATGAAGGCATTCCGCATCTGTTAACGGAAGTCGTTACTGATATGAAAGATGCCGCCAACgcgct                  >  2:434425/1‑151 (MQ=255)
                                                                                                                             cgATGATCCTGAGCATGCTTTATCAAGCACAGCCAGAAGATGTGCGTTTCATCATGATCGACCCGAAAATGCTGGAGCTTTCGGTTTATGAAGGCATTCCGCATCTGTTAACGGAAGTCGTTACTGATATGAAAGATGCCGCCAACgcgct                  <  2:890312/151‑1 (MQ=255)
                                                                                                                              gatgatCCTGAGCATGCTTTATCAAGCACAGCCAGAAGATGTGCGTTTCATCATGATCGACCCGAAAATGCTGGAGCTTTCGGTTTATGAAGGCATTCCGCATCTGTTAACGGAAGTCGTTACTGATATGAAAGATGCCGCCAACgcgctg                 >  1:902538/1‑151 (MQ=255)
                                                                                                                                       gAGCATGCTTTATCAAGCACAGCCAGAAGATGTGCGTTTCATCATGATCGACCCGAAAATGCTGGAGCTTTCGGTTTATGAAGGCATTCCGCATCTGTTAACGGAAGTCGTTACTGATATGAAAGATGCCGCCAACGCGCTGCGCTGgtgt        <  2:65919/151‑1 (MQ=255)
                                                                                                                                           aTGCTTTATCAAGCACAGCCAGAAGATGTGCGTTTCATCATGATCGACCCGAAAATGCTGGAGCTTTCGGTTTATGAAGGCATTCCGCATCTGTTAACGGAAGTCGTTACTGATATGAAAGATGCCGCCAACGCGCTGCGCTGGTGTGtt     >  1:202536/1‑150 (MQ=255)
                                                                                                                                             gCTTTATCAAGCACAGCCAGAAGATGTGCGTTTCATCATGATCGACCCGAAAATGCTGGAGCTTTCGGTTTATGAAGGCATTCCGCATCTGTTAACGGAAGTCGTTACTGATATGAAAGATGCCGCCAACGCGCTGCGCTGGTGTGTTAAc  <  1:788292/151‑1 (MQ=255)
                                                                                                                                             gCTTTATCAAGCACAGCCAGAAGATGTGCGTTTCATCATGATCGACCCGAAAATGCTGGAGCTTTCGGTTTATGAAGGCATTCCGCATCTGTTAACGGAAGTCGTTACTGATATGAAAGATGCCGCCAACGCGCTGCGCTGGTGTGTTAAc  <  1:781618/151‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                               
GTCGCCATTAACCGTGGTGCTGGGTAAAGATATCGCCGGTGAGCCGGTGGTTGCCGATCTGGCGAAAATGCCGCACTTGTTGGTTGCGGGGACTACCGGTTCCGGTAAATCTGTCGGTGTGAACGCGATGATCCTGAGCATGCTTTATAAAGCACAGCCAGAAGATGTGCGTTTCATCATGATCGACCCGAAAATGCTGGAGCTTTCGGTTTATGAAGGCATTCCGCATCTGTTAACGGAAGTCGTTACTGATATGAAAGATGCCGCCAACGCGCTGCGCTGGTGTGTTAAC  >  NC_000913/936106‑936397

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑