Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,052,447	T→G	K306Q (AAA→CAA)	yccM ←	putative 4Fe‑4S membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,052,447	0	T	G	100.0%	194.5 / NA	53	K306Q (AAA→CAA)	yccM	putative 4Fe‑4S membrane protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (26/27);  total (26/27)

ATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGGCACCGGGCTTTGCCATACTCCCGCATACATTGCGTAACCAATCACCGCAAACATAATCCCCAGAACCAGTAGCGTCATTAACCAGCCAGACAACGCAAAGGCTTTTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGGCTACCGGACATGACTCTACGCAAGTCATACAGCCGGTACATTCCACTGTTCGTACCTGAATTAATTTATCGACCGGGATCCGTGATGGGCAATTTTTTGCGC  >  NC_000913/1052299‑1052593                                                                                                                                                     |                                                                                                                                                   aTCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGGCACCGGGCTTTGCCATACTCCCGCATACATTGCGTAACCAATCACCGCAAACATAATCCCCAGAACCAGTAGCGTCATTAACCAGCCAGACAACGCAAAGGCttgtt                                                                                                                                                  <  2:457723/151‑1 (MQ=255)   cATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGGCACCGGGCTTTGCCATACTCCCGCATACATTGCGTAACCAATCACCGCAAACATAATCCCCAGAACCAGTAGCGTCATTATCCAGCCAGACAACGCAAAGGCttgttt                                                                                                                                                 <  2:848222/150‑1 (MQ=255)   cATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGGCACCGGGCTTTGCCATACTCCCGCATACATTGCGTAACCAATCACCGCAAACATAATCCCCAGAACCAGTAGCGTCATTAACCAGCCAGACAACGCAAAGGCttgttt                                                                                                                                                 <  1:381304/150‑1 (MQ=255)       ggCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGGCACCGGGCTTTGCCATACTCCCGCATACATTGCGTAACCAATCACCGCAAACATAATCCCCAGAACCAGTAGCGTCATTAACCAGCCAGACAACGCAAAGGCTTGTTTATTTg                                                                                                                                            >  1:200108/1‑151 (MQ=255)           cTTGTGGAATTAAGCGTCGGTACAATTCCTCCGGCACCGGGCTTTGCCATACTCCCGCATACATTGCGTAACCAATCACCGCAAACATAATCCCCAGAACCAGTAGCGTCATTAACCAGCCAGACAACGCAAAGGCTTGTTTATTTGCCGc                                                                                                                                        >  1:266534/1‑151 (MQ=255)             tgtgGAATTAAGCGTCGGTACAATTCCTCCGGCACCGGGCTTTGCCATACTCCCGCATACATTGCGTAACCAATCACCGCAAACATAATCCCCAGAACCAGTAGCGTCATTAACCAGCCAGACAACGCAAAGGCTTGTTTATTTGCCGc                                                                                                                                        >  2:383071/1‑149 (MQ=255)                 gAATTAAGCGTCGGTACAATTCCTCCGGCACCGGGCTTTGCCATACTCCCGCATACATTGCGTAACCAATCACCGCAAACATAATCCCCAGAACCAGTAGCGTCATTAACCAGCCAGACAACGCAAAGGCTTGTTTATTTGCCGCAGGttt                                                                                                                                  <  2:175487/151‑1 (MQ=255)                   aTTAAGCGTCGGTACAATTCCTCCGGCACCGGGCTTTGCCATACTCCCGCATACATTGCGTAACCAATCACCGCAAACATAATCCCCAGAACCAGTAGCGTCATTAACCAGCCAGACAACGCAAAGGCTTGTTTATTTGCCGCAGGttttt                                                                                                                                >  1:196943/1‑151 (MQ=255)                           tCGGTACAATTCCTCCGGCACCGGGCTTTGCCATACTCCCGCATACATTGCGTAACCAATCACCGCAAACATAATCCCCAGAACCAGTAGCGTCATTAACCAGCCAGACAACGCAAAGGCTTGTTTATTTGCCGCAGGTTTTTGCAGTGa                                                                                                                         >  1:573817/1‑150 (MQ=255)                                     tcctccGGCACCGGGCTTTGCCATACTCCCGCATACATTGCGTAACCAATCACCGCAAACATAATCCCCAGAACCAGTAGCGTCATTAACCAGCCAGACAACGCAAAGGCTTGTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATg                                                                                                              >  1:739848/1‑151 (MQ=255)                                         ccGGCACCGGGCTTTGCCATACTCCCGCATACATTGCGTAACCAATCACCGCAAACATAATCCCCAGAACCAGTAGCGTCATTAACCAGCCAGACAACGCAAAGGCTTGTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGttgattgct                                                                                                           <  1:274716/150‑9 (MQ=255)                                         ccGGCACCGGGCTTTGCCATACTCCCGCATACATTGCGTAACCAATCACCGCAAACATAATCCCCAGAACCAGTAGCGTCATTAACCAGCCAGACAACGCAAAGGCTTGTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTg                                                                                                           >  2:426876/1‑150 (MQ=255)                                          cGGCACCGGGCTTTGCCATACTCCCGCATACATTGCGTAACCAATCACCGCAAACATAATCCCCAGAACCAGTAGCGTCATTAACCAGCCAGACAACGCAAAGGCTTGTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAg                                                                                                         <  1:682361/151‑1 (MQ=255)                                                       tGCCATACTCCCGCATACATTGCGTAACCAATCACCGCAAACATAATCCCCAGAACCAGTAGCGTCATTAACCAGCCAGACAACGCAAAGGCTTGTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGGCTACCGGACAt                                                                                             <  2:440695/150‑1 (MQ=255)                                                             aCTCCCGCATACATTGCGTAACCAATCACCGCAAACATAATCCCCAGAACCAGTAGCGTCATTAACCAGCCAGACAACGCAAAGGCTTGTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGGCTACCGGACATGACTCTa                                                                                      <  1:744167/151‑1 (MQ=255)                                                                    cATACATTGCGTAACCAATCACCGCAAACATAATCCCCAGAACCAGTAGCGTCATTAACCAGCCAGACAACGCAAAGGCTTGTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGGCTACCGGACATGACTCTACGCAAGt                                                                               <  1:414524/151‑1 (MQ=255)                                                                       aCATTGCGTAACCAATCACCGCAAACATAATCCCCAGAACCAGTAGCGTCATTAACCAGCCAGACAACGCAAAGGCTTGTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGGCTACCGGACATGACTCTACGCAAGTCAt                                                                            >  1:548903/1‑151 (MQ=255)                                                                            gCGTAACCAATCACCGCAAACATAATCCCCAGAACCAGTAGCGTCATTAACCAGCCAGACAACGCAAAGGCTTGTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGGCTACCGGACATGACTCTACGCAAGTCATACAGc                                                                       <  1:122531/151‑1 (MQ=255)                                                                                 aCCAATCACCGCAAACATAATCCCCAGAACCAGTAGCGTCATTAACCAGCCAGACAACGCAAAGGCTTGTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGCCAATGTTGATGCTACCGGACATGACTCTACGCAAGTCATACAGCCGGt                                                                   >  1:187309/1‑150 (MQ=255)                                                                                         ccGCAAACATAATCCCCAGAACCAGTAGCGTCATTAACCAGCCAGACAACGCAAAGGCTTGTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGGCTACCGGACATGACTCTACGCAAGTCATACAGCCGGTACATTCCa                                                           <  1:903312/150‑1 (MQ=255)                                                                                          cGCAAACATAATCCCCAGAACCAGTAGCGTCATTAACCAGCCAGACAACGCAAAGGCTTGTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGGCTACCGGACATGACTCTACGCAAGTCATACAGCCGGTACATTCCACt                                                         <  2:734635/151‑1 (MQ=255)                                                                                          cGCAAACATAATCCCCAGAACCAGTAGCGTCATTAACCAGCCAGACAACGCAAAGGCTTGTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGGCTACCGGACATGACTCTACGCAAGTCATACAGCCGGTACATTCCACt                                                         >  1:452279/1‑151 (MQ=255)                                                                                            cAAACATAATCCCCAGAACCAGTAGCGTCATTAACCAGCCAGACAACGCAAAGGCTTGTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGGCTACCGGACATGACTCTACGCAAGTCATACAGCCGGTACATTCCACTGt                                                       >  2:484200/1‑151 (MQ=255)                                                                                            cAAACATAATCCCCAGAACCAGTAGCGTCATTAACCAGCCAGACAACGCAAAGGCTTGTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGGCTACCGGACATGACTCTACGCAAGTCATACAGCCGGTACATTCCACTGt                                                       <  1:186914/151‑1 (MQ=255)                                                                                            cAAACATAATCCCCAGAACCAGTAGCGTCATTAACCAGCCAGACAACGCAAAGGCTTGTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGGCTACCGGACATGACTCTACGCAAGTCATACAGCCGGTACATTCCACTGt                                                       <  2:195125/151‑1 (MQ=255)                                                                                              aaCATAATCCCCAGAACCAGTAGCGTCATTAACCAGCCAGACAACGCAAAGGCTTGTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGGCTACCGGACATGACTCTACGCAAGTCATACAGCCGGTACATTCCACTGTTc                                                     <  2:269606/151‑1 (MQ=255)                                                                                               aCATAATCCCCAGAACCAGTAGCGTCATTAACCAGCCAGACAACGCAAAGGCTTGTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGGCTACCGGACATGACTCTACGCAAGTCATACAGCCGGTACATTCCACTGTTCg                                                    >  2:8939/1‑151 (MQ=255)                                                                                                cATAATCCCCAGAACCAGTAGCGTCATTAACCAGCCAGACAACGCAAAGGCTTGTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGGCTACCGGACATGACTCTACGCAAGTCATACAGCCGGTACATTCCACTGTTCGt                                                   >  2:367726/1‑151 (MQ=255)                                                                                                  tAATCCCCAGAACCAGTAGCGTCATTAACCAGCCAGACAACGCAAAGGCTTGTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGGCTACCGGACATGACTCTACGCAAGTCATACAGCCGGTACATTCCACTGTTCGTAc                                                 >  2:766951/1‑151 (MQ=255)                                                                                                     tCCCCAGAACCAGTAGCGTCATTAACCAGCCAGACAACGCAAAGGCTTGTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGGCTACCGGACATGACTCTACGCAAGTCATACAGCCGGTACATTCCACTGTTCGTACCTg                                              >  1:760154/1‑151 (MQ=255)                                                                                                      ccccAGAACCAGTAGCGTCATTAACCAGCCAGACAACGCAAAGGCTTGTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGGCTACCGGACATGACTCTACGCAAGTCATACAGCCGGTACATTCCACTGTTCGTACCTg                                              >  2:936000/1‑150 (MQ=255)                                                                                                       cccAGAACCAGTAGCGTCATTAACCAGCCAGACAACGCAAAGGCTTGTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGGCTACCGGACATGACTCTACGCAAGTCATACAGCCGGTACATTCCACTGTTCGTACCTGaa                                            <  2:673160/151‑1 (MQ=255)                                                                                                       cccAGAACCAGTAGCGTCATTAACCAGCCAGACAACGCAAAGGCTTGTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGGCTACCGGACATGACTCTACGCAAGTCATACAGCCGGTACATTCCACTGTTCGTACCTGa                                             >  1:353084/1‑150 (MQ=255)                                                                                                          aGAACCAGTAGCGTCATTAACCAGCCAGACAACGCAAAGGCTTGTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGGCTACCGGACATGACTCTACGCAAGTCATACAGCCGGTACATTCCACTGTTCGTACCTGaatta                                         <  2:71427/151‑1 (MQ=255)                                                                                                            aaCCAGTAGCGTCATTAACCAGCCAGACAACGCAAAGGCTTGTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGGCTACCGGACATGACTCTACGCAAGTCATACAGCCGGTACATTCCACTGTTCGTACCTGaattaat                                       >  1:896503/1‑151 (MQ=255)                                                                                                             aCCAGTAGCGTCATTAACCAGCCAGACAACGCAAAGGCTTGTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGGCTACCGGACATGACTCTACGCAAGTCATACAGCCGGTACATTCCACTGTTCGTACCTGaattaat                                       <  2:642852/150‑1 (MQ=255)                                                                                                                     cGTCATTAACCAGCCAGACAACGCAAAGGCTTGTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGGCTACCGGACATGACTCTACGCAAGTCATACAGCCGGTACATTCCACTGTTCGTACCTGAATTAATTTATCGAcc                              <  2:644748/151‑1 (MQ=255)                                                                                                                       tCATTAACCAGCCAGACAACGCAAAGGCTTGTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGGCTACCGGACATGACTCTACGCAAGTCATACAGCCGGTACATTCCACTGTTCGTACCTGAATTAATTTATCGACCgg                            >  2:66390/1‑151 (MQ=255)                                                                                                                        cATTAACCAGCCAGACAACGCAAAGGCTTGTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGGCTACCGGACATGACTCTACGCAAGTCATACAGCCGGTACATTCCACTGTTCGTACCTGAATTAATTTATCGACCggg                           <  1:128323/151‑1 (MQ=255)                                                                                                                           tAACCAGCCAGACAACGCAAAGGCTTGTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGGCTACCGGACATGACTCTACGCAAGTCATACAGCCGGTACATTCCACTGTTCGTACCTGAATTAATTTATCGACCGGGAt                         <  1:61545/150‑1 (MQ=255)                                                                                                                           tAACCAGCCAGACAACGCAAAGGCTTGTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGGCTACCGGACATGACTCTACGCAAGTCATACAGCCGGTACATTCCACTGTTCGTACCTGAATTAATTTATCGACCGGGATc                        >  2:892614/1‑151 (MQ=255)                                                                                                                            aaCCAGCCAGACAACGCAAAGGCTTGTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGGCTACCGGACATGACTCTACGCAAGTCATACAGCCGGTACATTCCACTGTTCGTACCTGAATTAATTTATCGACCGGGATcc                       <  2:794194/151‑1 (MQ=255)                                                                                                                            aaCCAGCCAGACAACGCAAAGGCTTGTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGGCTACCGGACATGACTCTACGCAAGTCATACAGCCGGTACATTCCACTGTTCGTACCTGAATTAATTTATCGACCGGGATcc                       <  1:73955/151‑1 (MQ=255)                                                                                                                            aaCCAGCCAGACAACGCAAAGGCTTGTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGGCTACCGGACATGACTCTACGCAAGTCATACAGCCGGTACATTCCACTGTTCGTACCTGAATTAATTTATCGACCGGGATc                        <  2:624583/150‑1 (MQ=255)                                                                                                                                agccagACAACGCAAAGGCTTGTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGGGGCTACCGGACATGACTCTACGCAAGTCATACAGCCGGTACATTCCACTGTTCGTACCTGAATTAATTTATCGACCGGGATCCGTg                    >  2:767454/1‑150 (MQ=255)                                                                                                                                agccagACAACGCAAAGGCTTGTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGGCTACCGGACATGACTCTACGCAAGTCATACAGCCGGTACATTCCACTGTTCGTACCTGAATTAATTTATCGACCGGGATCCGTg                    >  2:653322/1‑150 (MQ=255)                                                                                                                                agccagACAACGCAAAGGCTTGTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGGCTACCGGACATGACTCTACGCAAGTCATACAGCCGGTACATTCCACTGTTCGTACCTGAATTAATTTATCGACCGGGATCCGTGa                   <  1:66452/151‑1 (MQ=255)                                                                                                                                agccagACAACGCAAAGGCTTGTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGGCTACCGGACATGACTCTACGCAAGTCATACAGCCGGTACATTCCACTGTTCGTACCTGAATTAATTTATCGACCGGGATCCGTGa                   <  2:522400/151‑1 (MQ=255)                                                                                                                                 gccagACAACGCAAAGGCTTGTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGGCTACCGGACATGACTCTACGCAAGTCATACAGCCGGTACATTCCACTGTTCGTACCTGAATTAATTTATCGACCGGGATCCGTGAt                  >  1:142571/1‑151 (MQ=255)                                                                                                                                    agACAACGCAAAGGCTTGTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGGCTACCGGACATGACTCTACGCAAGTCATACAGCCGGTACATTCCACTGTTCGTACCTGAATTAATTTATCGACCGGGATCCGTGATggg               >  2:318091/1‑151 (MQ=255)                                                                                                                                       cAACGCAAAGGCTTGTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGGCTACCGGACATGACTCTACGCAAGTCATACAGCCGGTACATTCCACTGTTCGTACCTGAATTAATTTATCGACCGGGATCCGTGATGGGCa             >  2:223470/1‑150 (MQ=255)                                                                                                                                             aaaGGCTTGTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGGCTACCGGACATGACTCTACGCAAGTCATACAGCCGGTACATTCCACTGTTCGTACCTGAATTAATTTATCGACCGGGATCCGTGATGGGCAAtttttt      <  1:459373/151‑1 (MQ=255)                                                                                                                                              aaGGCTTGTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGGCTACCGGACATGACTCTACGCAAGTCATACAGCCGGTACATTCCACTGTTCGTACCTGAATTAATTTATCGACCGGGATCCGTGATGGGCAATTTTTTg     <  2:492173/151‑1 (MQ=255)                                                                                                                                               aGGCTTGTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGGCTACCGGACATGACTCTACGCAAGTCATACAGCCGGTACATTCCACTGTTCGTACCTGAATTAATTTATCGACCGGGATCCGTGATGGGCAATTTTTTgc    <  2:266541/151‑1 (MQ=255)                                                                                                                                               aGGCTTGGTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGGCTACCGGACATGACTCTACGCAAGTCATACAGCCGGTACATTCCACTGTTCGTACCTGAATTAATTTATCGACCGGGATCCGTGATGGGCAATTTTTTgc    <  2:886032/151‑1 (MQ=255)                                                                                                                                                  cTTGTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGGCTACCGGACATGACTCTACGCAAGTCATACAGCCGGTACATTCCACTGTTCGTACCTGAATTAATTTATCGACCGGGATCCGTGATGGGCAATTTTTTgcgc  >  2:144920/1‑150 (MQ=255)                                                                                                                                                     |                                                                                                                                                   ATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGGCACCGGGCTTTGCCATACTCCCGCATACATTGCGTAACCAATCACCGCAAACATAATCCCCAGAACCAGTAGCGTCATTAACCAGCCAGACAACGCAAAGGCTTTTTTATTTGCCGCAGGTTTTTGCAGTGAAAAGGTCAATGTTGAGGCTACCGGACATGACTCTACGCAAGTCATACAGCCGGTACATTCCACTGTTCGTACCTGAATTAATTTATCGACCGGGATCCGTGATGGGCAATTTTTTGCGC  >  NC_000913/1052299‑1052593

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑