| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,194,916 | T→G | Q12H (CAA→CAC) | rluE ← | 23S rRNA pseudouridine(2457) synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,194,916 | 0 | T | G | 97.6% | 153.2 / ‑6.2 | 42 | Q12H (CAA→CAC) | rluE | 23S rRNA pseudouridine(2457) synthase |
| Reads supporting (aligned to +/- strand): ref base T (1/0); new base G (17/24); total (18/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.29e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTTGCGTCCGGCTTCATCGGTGAACTGCGGAAGAACATCGTAGGGTTTATTGAACAGGATCACACGCGTGGGCTGGTTTTCAGGTTTACGCCTGGTAGAACGTTGCGAGCTGAATC‑GCTTAACCTGGTGATTTCTAAAAGAAGTTTTTTGCATGGTATTTTCAGAGATTATGAATTGCCGCATTATAGCCTAATAACGCGCATCTTTCATGACGGCAAACAATAGGGTAGTATTGACAAGCCAATTACAAATCATTAACAAAAAATTGCTCTAAAGCATCCGTATCGCAGG > NC_000913/1194769‑1195059 | tttGCGTCCGGCTTCATCGGTGAACTGCGGAAGAACATCGTAGGGTTTATTGAACAGGATCACACGCGTGGGCTGGTTTTCAGGTTTACGCCTGGTAGAACGTTGCGAGCTGAATC‑GCTTAACCTGGTGATTTCTAAAAGAAGTTTTGTGc > 1:446052/1‑151 (MQ=255) tGCGTCCGGCTTCATCGGTGAACTGCGGAAGAACATCGTAGGGTTTATTGAACAGGATCACACGCGTGGGCTGGTTTTCAGGTTTACGCCTGGTAGAACGTTGCGAGCTGAATC‑GCTTAACCTGGTGATTTCTAAAAGAAGTTTTGTGCAt > 2:647707/1‑151 (MQ=255) cGTCCGGCTTCATCGGTGAACTGCGGAAGAACATCGTAGGGTTTATTGAACAGGATCACACGCGTGGGCTGGTTTTCAGGTTTACGCCTGGTAGAACGTTGCGAGCTGAATC‑GCTTAACCTGGTGATTTCTAAAAGAAGTTTTGTGCATgg < 1:749521/151‑1 (MQ=255) tCATCGGTGAACTGCGGAAGAACATCGTAGGGTTTATTGAACAGGATCACACGCGTGGGCTGGTTTTCAGGTTTACGCCTGGTAGAACGTTGCGAGCTGAATC‑GCTTAACCTGGTGATTTCTAAAAGAAGTTTTGTGCATGGTATTTTCa < 2:61984/150‑1 (MQ=255) cATCGGTGAACTGCGGAAGAACATCGTAGGGTTTATTGAACAGGATCACACGCGTGGGCTGGTTTTCAGGTTTACGCCTGGTAGAACGTTGCGAGCTGAATC‑GCTTAACCTGGTGATTTCTAAAAGAAGTTTTGTGCATGGTATTTTc > 2:28809/1‑148 (MQ=255) aTCGGTGAACTGCGGAAGAACATCGTAGGGTTTATTGAACAGGATCACACGCGTGGGCTGGTTTTCAGG‑TTACGCCTGGGAAGACGGTGCCAGCTGAATCGGCTTAA‑CTTGTGGTTTTTAAAAGAAGGTTTTGGGATGGGTTTTTCa > 1:891782/1‑147 (MQ=255) cGGTGAACTGCGGAAGAACATCGTAGGGTTTATTGAACAGGATCACACGCGTGGGCTGGTTTTCAGGTTTACGCCTGGTAGAACGTTGCGAGCTGAATC‑GCTTAACCTGGTGATTTCTAAAAGAAGTTTTGTGCATGGTATTTTCAGAGAt < 1:452251/151‑1 (MQ=255) gTGAACTGCGGAAGAACATCGTAGGGTTTATTGAACAGGATCACACGCGTGGGCTGGTTTTCAGGTTTACGCCTGGTAGAACGTTGCGAGCTGAATC‑GCTTAACCTGGTGATTTCTAAAAGAAGTTTTGTGCATGGTATTTTCAGAGATTa < 1:221618/151‑1 (MQ=255) gAACTGCGGAAGAACATCGTAGGGTTTATTGAACAGGATCACACGCGTGGGCTGGTTTTCAGGTTTACGCCTGGTAGAACGTTGCGAGCTGAATC‑GCTTAACCTGGTGATTTCTAAAAGAAGTTTTGTGCATGGTATTTTCAGAGATTAt < 2:760964/150‑1 (MQ=255) gAACTGCGGAAGAACATCGTAGGGTTTATTGAACAGGATCACACGCGTGGGCTGGTTTTCAGGTTTACGCCTGGTAGAACGTTGCGAGCTGAATC‑GCTTAACCTGGTGATTTCTAAAAGAAGTTTTGTGCATGGTATTTTCAGAGATTATg > 2:209428/1‑151 (MQ=255) cGGAAGAACATCGTAGGGTTTATTGAACAGGATCACACGCGTGGGCTGGTTTTCAGGTTTACGCCTGGTAGAACGTTGCGAGCTGAATC‑GCTTAACCTGGTGATTTCTAAAAGAAGTTTTGTGCATGGTATTTTCAGAGATTATGAAtt < 2:680328/149‑1 (MQ=255) gaagaaCATCGTAGGGTTTATTGAACAGGATCACACGCGTGGGCTGGTTTTCAGGTTTACGCCTGGTAGAACGTTGCGAGCTGAATC‑GCTTAACCTGGTGATTTCTAAAAGAAGTTTTGTGCATGGTATTTTCAGAGATTATGAATTGCCg > 2:634874/1‑151 (MQ=255) agaaCATCGTAGGGTTTATTGAACAGGATCACACGCGTGGGCTGGTTTTCAGGTTTACGCCTGGTAGAACGTTGCGAGCTGAATC‑GCTTAACCTGGTGATTTCTAAAAGAAGTTTTGTGCATGGTATTTTCAGAGATTATGAATTGCCGc > 2:769095/1‑150 (MQ=255) cATCGTAGGGTTTATTGAACAGGATCACACGCGTGGGCTGGTTTTCAGGTTTACGCCTGGTAGAACGTTGCGAGCTGAATC‑GCTTAACCTGGTGATTTCTAAAAGAAGTTTTGTGCATGGTATTTTCAGAGATTATGAATTGCCGCATtat > 1:316158/1‑151 (MQ=255) aTCGTAGGGTTTATTGAACAGGATCACACGCGTGGGCTGGTTTTCAGGTTTACGCCTGGTAGAACGTTGCGAGCTGAATC‑GCTTAACCTGGTGATTTCTAAAAGAAGTTTTGTGCATGGTATTTTCAGAGATTATGAATTGCCGCATtata < 2:107699/151‑1 (MQ=255) aGGGTTTATTGAACAGGATCACACGCGTGGGCTGGTTTTCAGGTTTACGCCTGGTAGAACGTTGCGAGCTGAATC‑GCTTAACCTGGTGATTTCTAAAAGAAGTTTTGTGCATGGTATTTTCAGAGATTATGAATTGCCGCATTATAGCCt < 1:465422/150‑1 (MQ=255) aTTGAACAGGATCACACGCGTGGGCTGGTTTTCAGGTTTACGCCTGGTAGAACGTTGCGAGCTGAATC‑GCTTAACCTGGTGATTTCTAAAAGAAGTTTTGTGCATGGTATTTTCAGAGATTATGAATTGCCGCATTATAGCCTAATAAcgc > 1:322589/1‑151 (MQ=255) cAGGATCACACGCGTGGGCTGGTTTTCAGGGTTACGCCTGGTAGAACGTTGCGAGCTGAATC‑GCTTAACCTGGTGATTTCTAAAAGAAGTTTTGTGCATGGTATTTTCAGAGATTATGAATTGCCGCATTATAGCCTAATAACGCGTAt < 1:745353/149‑1 (MQ=255) aGGATCACACGCGTGGGCTGGTTTTCAGGTTTACGCCTGGTAGAACGTTGCGAGCTGAATC‑GCTTAACCTGGTGATTTCTAAAAGAAGTTTTGTGCATGGTATTTTCAGAGATTATGAATTGCCGCATTATAGCCTAATAACGCGCATCtt < 2:754510/151‑1 (MQ=255) ggATCACACGCGTGGGCTGGTTTTCAGGTTTACGCCTGGTAGAACGTTGCGAGCTGAATC‑GCTTAACCTGGTGATTTCTAAATGAAGTTTTGTGCATGGTATTTTCAGAGATTCTGAATTGCCGCATTATAGCCTAATAACGCGCATCttt > 1:361188/1‑151 (MQ=255) ggATCACACGCGTGGGCTGGTTTTCAGGTTTACGCCTGGTAGAACGTTGCGAGCTGAATC‑GCTTAACCTGGTGATTTCTAAAAGAAGTTTTGTGCATGGTATTTTCAGAGATTATGAATTGCCGCATTATAGCCTAATAACGCGCATCttt > 2:639474/1‑151 (MQ=255) gCTGGTTTTCAGGTTTACGCCTGGTAGAACGTTGCGAGCTGAATC‑GCTTAACCTGGTGATTTCTAAAAGAAGTTTTGTGCATGGTATTTTCAGAGATTATGAATTGCCGCATTATAGCCTAATAACGCGCATCTTTCATGACGGCAAACaa < 2:486986/151‑1 (MQ=255) cAGGTTTACGCCTGGTAGAACGTTGCGAGCTGAATC‑GCTTAACCTGGTGATTTCTAAAAGAAGTTTTGTGCATGGTATTTTCAGAGATTATGAATTGCCGCATTATAGCCTAATAACGCGCATCTTTCATGACGGCAAACAATAGGgtagt < 2:571652/151‑1 (MQ=255) ggTTTACGCCTGGTAGAACGTTGCGAGCTGAATC‑GCTTAACCTGGTGATTTCTAAAAGAAGTTTTGTGCATGGTATTTTCAGAGATTATGAATTGCCGCATTATAGCCTAATAACGCGCATCTTTCATGACGGCAAACAATAGGGTAGTAt > 2:802/1‑151 (MQ=255) gTTTACGCCTGGTAGAACGTTGCGAGCTGAATC‑GCTTAACCTGGTGATTTCTAAAAGAAGTTTTGTGCATGGTATTTTCAGAGATTATGAATTGCCGCATTATAGCCTAATAACGCGCATCTTTCATGACGGCAAACAATAGGGTAGTAtt > 2:236116/1‑151 (MQ=255) gTTTACGCCTGGTAGAACGTTGCGAGCTGAATC‑GCTTAACCTGGTGATTTCTAAAAGAAGTTTTGTGCATGGTATTTTCAGAGATTATGAATTGCCGCATTATAGCCTAATAACGCGCATCTTTCATGACGGCAAACAATAGGGTAGTAtt > 2:374063/1‑151 (MQ=255) cGCCTGGTAGAACGTTGCGAGCTGAATC‑GCTTAACCTGGTGATTTCTAAAAGAAGTTTTGTGCATGGTATTTTCAGAGATTATGAATTGCCGCATTATAGCCTAATAACGCGCATCTTTCATGACGGCAAACAATAGGGTAGTATTGACa > 2:787746/1‑150 (MQ=255) cTGGTAGAACGTTGCGAGCTGAATC‑GCTTAACCTGGTGATTTCTAAAAGAAGTTTTGTGCATGGTATTTTCAGAGATTATGAATTGCCGCATTATAGCCTAATAACGCGCATCTTTCATGACGGCAAACAATAGGGTAGTATTGACAAGcc > 1:19245/1‑151 (MQ=255) ggTAGAACGTTGCGAGCTGAATC‑GCTTAACCTGGTGATTTCTAAAAGAAGTTTTGTGCATGGTATTTTCAGAGATTATGAATTGCCGCATTATAGCCTAATAACGCGCATCTTTCATGACGGCAAACAATAGGGTAGTATTGACAAGCCaa < 1:120382/151‑1 (MQ=255) ggTAGAACGTTGCGAGCTGAATC‑GCTTAACCTGGTGATTTCTAAAAGAAGTTTTGTGCATGGTATTTTCAGAGATTATGAATTGCCGCATTATAGCCTAATAACGCGCATCTTTCATGACGGCAAACAATAGGGTAGTATTGACAAGCCaa < 1:285888/151‑1 (MQ=255) aGAACGTTGCGAGCTGAATC‑GCTTAACCTGGTGATTTCTAAAAGAAGTTTTGTGCATGGTATTTTCAGAGATTATGAATTGCCGCATTATAGCCTAATAACGCGCATCTTTCATGACGGCAAACAATAGGGTAGTATTGACAAGCCAATTa < 2:529667/151‑1 (MQ=255) aaCGTTGCGAGCTGAATC‑GCTTAACCTGGTGATTTCTAAAAGAAGTTTTGTGCATGGTATTTTCAGAGATTATGAATTGCCGCATTATAGCCTAATAACGCGCATCTTTCATGACGGCAAACAATAGGGTAGTATTGACAAGCCAATTACa < 1:128299/151‑1 (MQ=255) gTTGCGAGCTGAATC‑GCTTAACCTGGTGATTTCTAAAAGAAGTTTTGTGCATGGTATTTTCAGAGATTATGAATTGCCGCATTATAGCCTAATAACGCGCATCTTTCATGACGGCAAACAATAGGGTAGTATTGACAAGCCAATTACAAAt < 2:520920/151‑1 (MQ=255) aaTC‑GCTTAACCTGGTGATTTCTAAAAGAAGTTTTGTGCATGGTATTTTCAGAGATTATGAATTGCCGCATTATAGCCTAATAACGCGCATCTTTCATGACGGCAAACAATAGGGTAGTATTGACAAGCCAATTACAAATCATTAACaaaa < 2:372580/151‑1 (MQ=255) c‑GCTTAACCTGGTGATTTCTAAAAGAAGTTTTGTGCATGGTATTTTCAGAGATTATGAATTGCCGCATTATAGCCTAATAACGCGCATCTTTCATGACGGCAAACAATAGGGTAGTATTGACAAGCCAATTACAAATCATTAACAAAAAAt < 1:797026/151‑1 (MQ=255) c‑GCTTAACCTGGTGATTTCTAAAAGAAGTTTTGTGCATGGTATTTTCAGAGATTATGAATTGCCGCATTATAGCCTAATAACGCGCATCTTTCATGACGGCAAACAATAGGGTAGTATTGACAAGCCAATTACAAATCATTAACAAAAAAt < 1:700982/151‑1 (MQ=255) cTTAACCTGGTGATTTCTAAAAGAAGTTTTGTGCATGGTATTTTCAGAGATTATGAATTGCCGCATTATAGCCTAATAACGCGCATCTTTCATGACGGCAAACAATAGGGTAGTATTGACAAGCCAATTACAAATCATTAACAAAAAATTg > 1:11597/1‑151 (MQ=255) tAACCTGGTGATTTCTAAAAGAAGTTTTGTGCATGGTATTTTCAGAGATTATGAATTGCCGCATTATAGCCTAATAACGCGCATCTTTCATGACGGCAAACAATAGGGTAGTATTGACAAGCCAATTACAAATCATTAACAAAAAATTg < 1:791725/149‑1 (MQ=255) tAACCTGGTGATTTCTAAAAGAAGTTTTGTGCATGGTATTTTCAGAGATTATGAATTGCCGCATTATAGCCTAATAACGCGCATCTTTCATGACGGCAAACAATAGGGTAGTATTGACAAGCCAATTACAAATCATTAACAAAAAATTGc < 2:601383/150‑1 (MQ=255) aaCCTGGTGATTTCTAAAAGAAGTTTTGTGCATGGTATTTTCAGAGATTATGAATTGCCGCATTATAGCCTAATAACGCGCATCTTTCATGACGGCAAACAATAGGGTAGTATTGACAAGCCAATTACAAATCATTAACAAAAAATTGctc < 1:420309/151‑1 (MQ=255) gaagTTTTGTGCATGGTATTTTCAGAGATTATGAATTGCCGCATTATAGCCTAATAACGCGCATCTTTCATGACGGCAAACAATAGGGTAGTATTGACAAGCCAATTACAAATCATTAACAAAAAATTGCTCTAAAGCATCCGTATCGCAg < 2:633702/151‑1 (MQ=255) agTTTTGTGCATGGTATTTTCAGAGATTATGAATTGCCGCATTATAGCCTAATAACGCGCATCTTTCATGACGGCAAACAATAGGGTAGTATTGACAAGCCAATTACAAATCATTAACAAAAAATTGCTCTAAAGCATCCGTATCGCAgg > 2:533997/1‑150 (MQ=255) | TTTGCGTCCGGCTTCATCGGTGAACTGCGGAAGAACATCGTAGGGTTTATTGAACAGGATCACACGCGTGGGCTGGTTTTCAGGTTTACGCCTGGTAGAACGTTGCGAGCTGAATC‑GCTTAACCTGGTGATTTCTAAAAGAAGTTTTTTGCATGGTATTTTCAGAGATTATGAATTGCCGCATTATAGCCTAATAACGCGCATCTTTCATGACGGCAAACAATAGGGTAGTATTGACAAGCCAATTACAAATCATTAACAAAAAATTGCTCTAAAGCATCCGTATCGCAGG > NC_000913/1194769‑1195059 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |