Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	62,084	T→G	E394A (GAA→GCA)	rapA ←	RNA polymerase remodeling/recycling factor ATPase; RNA polymerase‑associated, ATP‑dependent RNA translocase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	62,084	0	T	G	100.0%	168.0 / NA	45	E394A (GAA→GCA)	rapA	RNA polymerase remodeling/recycling factor ATPase; RNA polymerase‑associated, ATP‑dependent RNA translocase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (21/24);  total (21/24)

GCGTCGGTAGCGGCAGCTTAATGGTGTGCAGCTCGCGTTTCGGGAATCCTTTCACACCGTTACGCGTGTTACGGAACAGCACGCGGCTGGTGCCGTGGCGATCCATCAGCATCGAAACCAGCTCCTGACGGGCGCTCTGGGCATCTTCGCTGTCGCTGTTTGCTGCCTGCAACAGCGGCTCGATATCCTGCTCGCCGATCATCTCGCCGAGCATGTTCAGTTCGTCATTGCTCAGTTTGTTACCTGCCAGCAGCATGGCAACGGCGTCCGCAACCGGACGATAATTTTTCTGC  >  NC_000913/61938‑62230                                                                                                                                                   |                                                                                                                                                   gCGTCGGTAGCGGCAGCTTAATGGTGTGCAGCTCGCGTTTCGGGAATCCTTTCACACCGTTACGCGTGTTACGGAACAGCACGCGGCTGGTGCCGTGGCGATCCATCAGCATCGAAACCAGCTCCTGACGGGCGCTCTGGGCATCTgcgct                                                                                                                                                <  2:786809/151‑1 (MQ=255) gCGTCGGTAGCGGCAGCTTAATGGTGTGCAGCTCGCGTTTCGGGAATCCTTTCACACCGTTACGCGTGTTACGGAACAGCACGCGGCTGGTGCCGTGGCGATCCATCAGCATCGAAACCAGCTCCTGACGGGCGCTCTGGGCATCTgcg                                                                                                                                                  <  1:156512/149‑1 (MQ=255)  cGTCGGTAGCGGCAGCTTAATGGTGTGCAGCTCGCGTTTCGGGAATCCTTTCACACCGTTACGCGTGTTACGGAACAGCACGCGGCTGGTGCCGTGGCGATCCATCAGCATCGAAACCAGCTCCTGACGGGCGCTCTGGGCATCTgcgctg                                                                                                                                               <  2:26781/151‑1 (MQ=255)    tCGGTAGCGGCAGCTTAATGGTGTGCAGCTCGCGTTTCGGGAATCCTTTCACACCGTTACGCGTGTTACGGAACAGCACGCGGCTGGTGCCGTGGCGATCCATCAGCATCGAAACCAGCTCCTGACGGGCGCTCTGGGCATCTgcgctgtc                                                                                                                                             >  2:805129/1‑151 (MQ=255)             gCAGCTTAATGGTGTGCAGCTCGCGTTTCGGGAATCCTTTCACACCGTTACGCGTGTTACGGAACAGCACGCGGCTGGTGCCGTGGCGATCCATCAGCATCGAAACCAGCTCCTGACGGGCGCTCTGGGCATCTGCGCTGTCGCTGTTtgc                                                                                                                                    <  1:49766/151‑1 (MQ=255)              cAGCTTAATGGTGTGCAGCTCGCGTTCCGGGAATCCTTTCACACCGTTACGCGTGTTACGGAACAGCACGCGGCTGGTGCCGTGGCGATCCATCAGCATCGAAACCAGCTCCTGACGGGCGCTCTGGGCATCTGCGCTGTCGCTGTTtgct                                                                                                                                   >  2:436844/1‑151 (MQ=255)               aGCTTAATGGTGTGCAGCTCGCGTTTCGGGAATCCTTTCACACCGTTACGCGTGTTACGGAACAGCACGCGGCTGGTGCCGTGGCGATCCATCAGCATCGAAACCAGCTCCTGACGGGCGCTCTGGGCATCTGCGCTGTCGCTGTTtgctg                                                                                                                                  >  2:548409/1‑151 (MQ=255)                   tAATGGTGTGCAGCTCGCGTTTCGGGAATCCTTTCACACCGTTACGCGTGTTACGGAACAGCACGCGGCTGGTGCCGTGGCGATCCATCAGCATCGAAACCAGCTCCTGACGGGCGCTCTGGGCATCTGCGCTGTCGCTGTTTGctgcctg                                                                                                                              >  2:621236/1‑151 (MQ=255)                    aaTGGTGTGCAGCTCGCGTTTCGGGAATCCTTTCACACCGTTACGCGTGTTACGGAACAGCACGCGGCTGGTGCCGTGGCGATCCATCAGCATCGAAACCAGCTCCTGACGGGCGCTCTGGGCATCTGCGCTGTCGCTGTTTGctgcctg                                                                                                                              <  1:74084/150‑1 (MQ=255)                     aTGGTGTGCAGCTCGCGTTTCGGGAATCCTTTCACACCGTTACGCGTGTTACGGAACAGCACGCGGCTGGTGCCGTGGCGATCCATCAGCATCGAAACCAGCTCCTGACGGGCGCTCTGGGCATCTGCGCTGTCGCTGTTTGctgcctgc                                                                                                                             >  1:393094/1‑150 (MQ=255)                          gtgCAGCTCGCGTTTCGGGAATCCTTTCACACCGTTACGCGTGTTACGGAACAGCACGCGGCTGGTGCCGTGGCGATCCATCAGCATCGAAACCAGCTCCTGACGGGCGCTCTGGGCATCTGCGCTGTCGCTGTTTGCTGCCTGCAACAGc                                                                                                                       <  2:493776/151‑1 (MQ=255)                             cAGCTCGCGTTTCGGGAATCCTTTCACACCGTTACGCGTGTTACGGAACAGCACGCGGCTGGTGCCGTGGCGATCCATCAGCATCGAAGCCAGCTCCTGACGGGCGCTCTGGGCATCTGCGCTGTCGCTGTTTGCTGCCTGCAACAGCGGc                                                                                                                    <  2:252897/151‑1 (MQ=255)                               gCTCGCGTTTCGGGAATCCTTTCACACCGTTACGCGTGTTACGGAACAGCACGCGGCTGGTGCCGTGGCGATCCATCAGCATCGAAACCAGCTCCTGACGGGCGCTCTGGGCATCTGCGCTGTCGCTGTTTGCTGCCTGCAACAGCGGCTc                                                                                                                  <  1:54505/151‑1 (MQ=255)                                cTCGCGTTTCGGGAATCCTTTCACACCGTTACGCGTGTTACGGAACAGCACGCGGCTGGTGCCGTGGCGATCCATCAGCATCGAAACCAGCTCCTGACGGGCGCTCTGGGCATCTGCGCTGTCGCTGTTTGCTGCCTGCAACAGCGGCTc                                                                                                                  >  1:302517/1‑150 (MQ=255)                                 tCGCGTTTCGGGAATCCTTTCACACCGTTACGCGTGTTACGGAACAGCACGCGGCTGGTGCCGTGGCGATCCATCAGCATCGAAACCAGCTCCTGACGGGCGCTCTGGGCATCTGCGCTGTCGCTGTTTGCTGCCTGCAACAGCGGCTCg                                                                                                                 >  1:543420/1‑150 (MQ=255)                                     gTTTCGGGAATCCTTTCACACCGTTACGCGTGTTACGGAACAGCACGCGGCTGGTGCCGTGGCGATCCATCAGCATCGAAACCAGCTCCTGACGGGCGCTCTGGGCATCTGCGCTGTCGCTGTTTGCTGCCTGCAACAGCGGCTCGATATc                                                                                                            <  1:99397/151‑1 (MQ=255)                                        tCGGGAATCCTTTCACACCGTTACGCGTGTTACGGAACAGCACGCGGCTGGTGCCGTGGCGATCCATCAGCATCGAAACCAGCTCCTGACGGGCGCTCTGGGCATCTGCGCTGTCGCTGTTTGCTGCCTGCAACAGCGGCTCGATATCCt                                                                                                          <  1:743098/150‑1 (MQ=255)                                        tCGGGAATCCTTTCACACCGTTACGCGTGTTACGGAACAGCACGCGGCTGGTGCCGTGGCGATCCATCAGCATCGAAACCAGCTCCTGACGGGCGCTCTGGGCATCTGCGCTGTCGCTGTTTGCTGCCTGCAACAGCGGCTCGATATCCt                                                                                                          <  2:874629/150‑1 (MQ=255)                                           ggAATCCTTTCACACCGTTACGCGTGTTACGGAACAGCACGCGGCTGGTGCCGTGGCGATCCATCAGCATCGAAACCAGCTCCTGACGGGCGCTCTGGGCATCTGCGCTGTCGCTGTTTGCTGCCTGCAACAGCGGCTCGATATCCTGCTc                                                                                                      <  1:560107/151‑1 (MQ=255)                                              aTCCTTTCACACCGTTACGCGTGTTACGGAACAGCACGCGGCTGGTGCCGTGGCGATCCATCAGCATCGAAACCAGCTCCTGACGGGCGCTCTGGGCATCTGCGCTGTCGCTGTTTGCTGCCTGCAACAGCGGCTCGATATCCTGCTCGcc                                                                                                   >  1:577592/1‑151 (MQ=255)                                              aTCCTTTCACACCGTTACGCGTGTTACGGAACAGCACGCGGCTGGTGCCGTGGCGATCCATCAGCATCGAAACCAGCTCCTGACGGGCGCTCTGGGCATCTGCGCTGTCGCTGTTTGCTGCCTGCAACAGCGGCTCGATATCCTGCTCGcc                                                                                                   <  2:429699/151‑1 (MQ=255)                                                  tttCACACCGTTACGCGTGTTACGGAACAGCACGCGGCTGGTGCCGTGGCGATCCATCAGCATCGAAACCAGCTCCTGACGGGCGCTCTGGGCATCTGCGCTGTCGCTGTTTGCTGCCTGCAACAGCGGCTCGATATCCTGCTCGCCGatc                                                                                               >  1:17224/1‑151 (MQ=255)                                                   ttCACACCGTTACGCGTGTTACGGAACAGCACGCGGCTGGTGCCGTGGCGATCCATCAGCATCGAAACCAGCTCCTGACGGGCGCTCTGGGCATCTGCGCTGTCGCTGTTTGCTGCCTGCAACAGCGGCTCGATATCCTGCTCGCCGat                                                                                                <  1:472584/149‑1 (MQ=255)                                                    tCACACCGTTACGCGTGTTACGGAACAGCACGCGGCTGGTGCCGTGGCGATCCATCAGCATCGAAACCAGCTCCTGACGGGCGCTCTGGGCATCTGCGCTGTCGCTGTTTGCTGCCTGCAACAGCGGCTCGATATCCTGCTCGCCGatca                                                                                              <  1:704778/150‑1 (MQ=255)                                                      acacCGTTACGCGTGTTACGGAACAGCACGCGGCTGGTGCCGTGGCGATCCATCAGCATCGAAACCAGCTCCTGACGGGCGCTCTGGGCATCTGCGCTGTCGCTGTTTGCTGCCTGCAACAGCGGCTCGATATCCTGCTCGCCGATCAtct                                                                                           >  2:219871/1‑151 (MQ=255)                                                          cGTTACGCGTGTTACGGAACAGCACGCGGCTGGTGCCGTGGCGATCCATCAGCATCGAAACCAGCTCCTGACGGGCGCTCTGGGCATCTGCGCTGTCGCTGTTTGCTGCCTGCAACAGCGGCTCGATATCCTGCTCGCCGATCATCTCGcc                                                                                       >  2:94014/1‑151 (MQ=255)                                                               cgcgTGTTACGGAACAGCACGCGGCTGGTGCCGTGGCGATCCATCAGCATCGAAACCAGCTCCTGACGGGCGCTCTGGGCATCTGCGCTGTCGCTGTTTGCTGCCTGCAACAGCGGCTCGATATCCTGCTCGCCGATCATCTCGCCGAGCa                                                                                  <  2:23928/151‑1 (MQ=255)                                                                gcgTGTTACGGAACAGCCCGCGGCTGGTGCCGTGGCGATCCATCAGCATCGAAACCAGCTCCTGACGGGCGCTCTGGGCATCTGCGCTGTCGCTGTTTGCTGCCTGCAACAGCGGCTCGATATCCTGCTCGCCGATCATCTCGCCGAGCa                                                                                  <  1:750565/150‑1 (MQ=255)                                                                    gtTACGGAACAGCACGCGGCTGGTGCCGTGGCGATCCATCAGCATCGAAACCAGCTCCTGACGGGCGCTCTGGGCATCTGCGCTGTCGCTGTTTGCTGCCTGCAACAGCGGCTCGATATCCTGCTCGCCGATCATCTCGCCGAGCATGTTc                                                                             <  2:798198/151‑1 (MQ=255)                                                                           aaCAGCACGCGGCTGGTGCCGTGGCGATCCATCAGCATCGAAACCAGCTCCTGACGGGCGCTCTGGGCATCTGCGCTGTCGCTGTTTGCTGCCTGCAACAGCGGCTCGATATCCTGCTCGCCGATCATCTCGCCGAGCATGTTCAGTTCGt                                                                      >  1:339271/1‑151 (MQ=255)                                                                            aCAGCACGCGGCTGGTGCCGTGGCGATCCATCAGCATCGAAACCAGCTCCTGACGGGCGCTCTGGGCATCTGCGCTGTCGCTGTTTGCTGCCTGCAACAGCGGCTCGATATCCTGCTCGCCGATCATCTCGCCGAGCATGTTCAGTTCGTc                                                                     >  1:154715/1‑151 (MQ=255)                                                                               gCACGCGGCTGGTGCCGTGGCGATCCATCAGCATCGAAACCAGCTCCTGACGGGCGCTCTGGGCATCTGCGCTGTCGCTGTTTGCTGCCTGCAACAGCGGCTCGATATCCTGCTCGCCGATCATCTCGCCGAGCATGTTCAGTTCGTCAtt                                                                  <  2:864698/151‑1 (MQ=255)                                                                               gCACGCGGCTGGTGCCGTGGCGATCCATCAGCATCGAAACCAGCTCCTGACGGGCGCTCTGGGCATCTGCGCTGTCGCTGTTTGCTGCCTGCAACAGCGGCTCGATATCCTGCTCGCCGATCATCTCGCCGAGCATGTTCAGTTCGTCAtt                                                                  <  2:605840/151‑1 (MQ=255)                                                                                   gcgGCTGGTGCCGTGGCGATCCATCAGCATCGAAACCAGCTCCTGACGGGCGCTCTGGGCATCTGCGCTGTCGCTGTTTGCTGCCTGCAACAGCGGCTCGATATCCTGCTCGCCGATCATCTCGCCGAGCATGTTCAGTTCGTCATTGCTc                                                              >  1:366307/1‑151 (MQ=255)                                                                                            gCCGTGGCGATCCATCAGCATCGAAACCAGCTCCTGACGGGCGCTCTGGGCATCTGCGCTGTCGCTGTTTGCTGCCTGCAACAGCGGCTCGATATCCTGCTCGCCGATCATCTCGCCGAGCATGTTCAGTTCGTCATTGCTCAGTTTGTTa                                                     <  2:638962/151‑1 (MQ=255)                                                                                              cGTGGCGATCCATCAGCATCGAAACCAGCTCCTGACGGGCGCTCTGGGCATCTGCGCTGTCGCTGTTTGCTGCCTGCAACAGCGGCTCGATATCCTGCTCGCCGATCATCTCGCCGAGCATGTTCAGTTCGTCATTGCTCAGTTTGTTAcc                                                   <  2:163829/151‑1 (MQ=255)                                                                                                  gCGATCCATCAGCATCGAAACCAGCTCCTGACGGGCGCTCTGGGCATCTGCGCTGTCGCTGTTTGCTGCCTGCAACAGCGGCTCGATATCCTGCTCGCCGATCATCTCGCCGAGCATGTTCAGTTCGTCATTGCTCAGTTTGTTACCTGcc                                               >  1:17979/1‑151 (MQ=255)                                                                                                       ccATCAGCATCGAAACCAGCTCCTGACGGGCGCTCTGGGCATCTGCGCTGTCGCTGTTTGCTGCCTGCAACAGCGGCTCGATATCCTGCTCGCCGATCATCTCGCCGAGCATGTTCAGTTCGTCATTGCTCAGTTTGTTACCTGCcagc                                            >  1:501256/1‑149 (MQ=255)                                                                                                           cAGCATCGAAACCAGCTCCTGACGGGCGCTCTGGGCATCTGCGCTGTCGCTGTTTGCTGCCTGCAACAGCGGCTCGATATCCTGCTCGCCGATCATCTCGCCGAGCATGTTCAGTTCGTCATTGCTCAGTTTGTTACCTGCCAGCAGCATg                                      <  1:840903/151‑1 (MQ=255)                                                                                                                          cTCCTGACGGGCGCTCTGGGCATCTGCGCTGTCGCTGTTTGCTGCCTGCAACAGCGGCTCGATATCCTGCTCGCCGATCATCTCGCCGAGCATGTTCAGTTCGTCATTGCTCAGTTTGTTACCTGCCAGCAGCATGGCAACGGCGTCCGc                        >  1:388021/1‑150 (MQ=255)                                                                                                                            ccTGACGGGCGCTCTGGGCATCTGCGCTGTCGCTGTTTGCTGCCTGCAACAGCGGCTCGATATCCTGCTCGCCGATCATCTCGCCGAGCATGTTCAGTTCGTCATTGCTCAGTTTGTTACCTGCCAGCAGCATGGCAACGGCGTCCGCaa                      <  2:94478/150‑1 (MQ=255)                                                                                                                            ccTGACGGGCGCTCTGGGCATCTGCGCTGTCGCTGTTTGCTGCCTGCAACAGCGGCTCGATATCCTGCTCGCCGATCATCTCGCCGAGCATGTTCAGTTCGTCATTGCTCAGTTTGTTACCTGCCAGCAGCATGGCAACGGCGTCCGCAAc                     <  2:669394/151‑1 (MQ=255)                                                                                                                               gACGGGCGCTCTGGGCATCTGCGCTGTCGCTGTTTGCTGCCTGCAACAGCGGCTCGATATCCTGCTCGCCGATCATCTCGCCGAGCATGTTCAGTTCGTCATTGCTCAGTTTGTTACCTGCCAGCAGCATGGCAACGGCGTCCGCAAcc                    <  2:375311/149‑1 (MQ=255)                                                                                                                                 cGGGCGCTCTGGGCATCTGCGCTGTCGCTGTTTGCTGCCTGCAACAGCGGCTCGATATCCTGCTCGCCGATCATCTCGCCGAGCATGTTCAGTTCGTCATTGCTCAGTTTGTTACCTGCCAGCAGCATGGCAACGGCGTCCGCAACCGGAc                <  1:875182/151‑1 (MQ=255)                                                                                                                                        tctGGGCATCTGCGCTGTCGCTGTTTGCTGCCTGCAACAGCGGCTCGATATCCTGCTCGCCGATCATCTCGCCGAGCATGTTCAGTTCGTCATTGCTCAGTTTGTTACCTGCCAGCAGCATGGCAACGGCGTCCGCAACCGGACGATAAtt         >  2:776505/1‑151 (MQ=255)                                                                                                                                        tctGGGCATCTGCGCTGTCGCTGTTTGCTGCCTGCAACAGCGGCTCGATATCCTGCTCGCCGATCATCTCGCCGAGCATGTTCAGTTCGTCATTGCTCAGTTTGTTACCTGCCAGCAGCATGGCAACGGCGTCCGCAACCGGACGATAAtt         >  1:876119/1‑151 (MQ=255)                                                                                                                                              cATCTGCGCTGTCGCTGTTTGCTGCCTGCAACAGCGGCTCGATATCCTGCTCGCCGATCATCTCGCCGAGCATGTTCAGTTCGTCATTGCTCAGTTTGTTACCTGCCAGCAGCATGGCAACGGCGTCCGCAACCGGACGATAATTTTTc     >  1:789756/1‑149 (MQ=255)                                                                                                                                               aTCTGCGCTGTCGCTGTTTGCTGCCTGCAACAGCGGCTCGATATCCTGCTCGCCGATCATCTCGCCGAGCATGTTCAGTTCGTCATTGCTCAGTTTGTTACCTGCCAGCAGCATGGCAACGGCGTCCGCAACCGGACGATAATTTTTCTGc  >  2:51823/1‑151 (MQ=255)                                                                                                                                               aTCTGCGCTGTCGCTGTTTGCTGCCTGCAACAGCGGCTCGATATCCTGCTCGCCGATCATCTCGCCGAGCATGTTCAGTTCGTCATTGCTCAGTTTGTTACCTGCCAGCAGCATGGCAACGGCGTCCGCAACCGGACGATAATTTTTCTGc  >  1:101693/1‑151 (MQ=255)                                                                                                                                                   |                                                                                                                                                   GCGTCGGTAGCGGCAGCTTAATGGTGTGCAGCTCGCGTTTCGGGAATCCTTTCACACCGTTACGCGTGTTACGGAACAGCACGCGGCTGGTGCCGTGGCGATCCATCAGCATCGAAACCAGCTCCTGACGGGCGCTCTGGGCATCTTCGCTGTCGCTGTTTGCTGCCTGCAACAGCGGCTCGATATCCTGCTCGCCGATCATCTCGCCGAGCATGTTCAGTTCGTCATTGCTCAGTTTGTTACCTGCCAGCAGCATGGCAACGGCGTCCGCAACCGGACGATAATTTTTCTGC  >  NC_000913/61938‑62230

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑