Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,217,877	A→C	T184P (ACG→CCG)	ycgG →	putative membrane‑anchored cyclic‑di‑GMP phosphodiesterase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,217,877	0	A	C	100.0%	212.3 / NA	59	T184P (ACG→CCG)	ycgG	putative membrane‑anchored cyclic‑di‑GMP phosphodiesterase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (34/25);  total (34/25)

ATCAGAACCAATTTGCAGATACGCGCATTTTGGTTACGATAAGCGATCAGCATATTCGTGGGGCACTTAATGTACCCTTGAAAGGGGTAAGGTATGTATTACGCGTGGCGGATGACATTATTGGACCAACGGGTGATGTGATGACGCTTAATGGACATTATCCCTATACCGAGAAGGTTCACTCCACAAAATATCATTTCACTATTATCTTTAACCCGCCGCCACTCTTTAGCTTCTACAGACTTATCGATAAAGGCTTTGGGATTTTAATATTTATTCTGTTAATCGCC  >  NC_000913/1217734‑1218023                                                                                                                                                |                                                                                                                                                   aTCAGAACCAATTTGCAGATACGCGCATTTTGGTTACGATAAGCGATCAGCATATTCGTGGGGCACTTAATGTACCCTTGAAAGGGGTAAGGTATGTATTACGCGTGGCGGATGACATTATTGGACCAACGGGTGATGTGATGCCGCtt                                                                                                                                               <  2:727551/149‑1 (MQ=255)   cAGAACCAATTTGCAGATACGCGCATTTTGGTTACGATAAGCGATCAGCATATTCGTGGGGCACTTAATGTACCCTTGAAAGGGGTAAGGTATGTATTACGCGTGGCGGATGACATTATTGGACCAACGGGTGATGTGATGCCGCTTAATg                                                                                                                                           >  2:822326/1‑151 (MQ=255)        ccAATTTGCAGATACGCGCATTTTGGTTACGATAAGCGATCAGCATATTCGTGGGGCACTTAATGTACCCTTGAAAGGGGTAAGGTATGTATTACGCGTGGCGGATGACATTATTGGACCAACGGGTGATGTGATGCCGCTTAATGGACAt                                                                                                                                      >  2:566579/1‑151 (MQ=255)        ccAATTTGCAGATACGCGCATTTTGGTTACGATAAGCGATCAGCATATTCGTGGGGCACTTAATGTACCCTTGAAAGGGGTAAGGTATGTATTACGCGTGGCGGATGACATTATTGGACCAACGGGTGATGTGATGCCGCTTAATGGACAt                                                                                                                                      <  1:599529/151‑1 (MQ=255)         cAATTTGCAGATACGCGCATTTTGGTTACGATAAGCGATCAGCATATTCGTGGGGCACTTAATGTACCCTTGAAAGGGGTAAGGTATGTATTACGCGTGGCGGATGACATTATTGGACCAACGGGTGATGTGATGCCGCTTAATGGACAtt                                                                                                                                     >  2:775281/1‑151 (MQ=255)         cAATTTGCAGATACGCGCATTTTGGTTACGATAAGCGATCAGCATATTCGTGGGGCACTTAATGTACCCTTGAAAGGGGTAAGGTATGTATTACGCGTGGCGGATGACATTATTGGACCAACGGGTGATGTGATGCCGCTTAATGGACAtt                                                                                                                                     <  1:882953/151‑1 (MQ=255)                  gATACGCGCATTTTGGTTACGATAAGCGATCAGCATATTCGTGGGGCACTTAATGTACCCTTGAAAGGGGTAAGGTATGTATTACGCGTGGCGGATGACATTATTGGACCAACGGGTGATGTGATGCCGCTTAATGGACATTATCCCtata                                                                                                                            <  1:15445/151‑1 (MQ=255)                       gcgcATTTTGGTTACGATAAGCGATCAGCATATTCGTGGGGCACTTAATGTACCCTTGAAAGGGGTAAGGTATGTATTACGCGTGGCGGATGACATTATTGGACCAACGGGTGATGTGATGCCGCTTAATGGACATTATCCCTATACCg                                                                                                                         >  1:496958/1‑149 (MQ=255)                          cATTTTGGTTACGATAAGCGATCAGCATATTCGTGGGGCACTTAATGTACCCTTGAAAGGGGTAAGGTATGTATTACGCGTGGCGGATGACATTATTGGACCAACGGGTGATGTGATGCCGCTTAATGGACATTATCCCTATACCGAGAAg                                                                                                                    <  1:305699/151‑1 (MQ=255)                          cATTTTGGTTACGATAAGCGATCAGCATATTCGTGGGGCACTTAATGTACCCTTGAAAGGGGTAAGGTATGTATTACGCGTGGCGGATGACATTATTGGACCAACGGGTGATGTGATGCCGCTTAATGGACATTATCCCTATACCGAGAAg                                                                                                                    <  1:906022/151‑1 (MQ=255)                           aTTTTGGTTACGATAAGCGATCAGCATATTCGTGGGGCACTTAATGTACCCTTGAAAGGGGTAAGGTATGTATTACGCGTGGCGGATGACATTATTGGACCAACGGGTGATGTGATGCCGCTTAATGGACATTATCCCTATACCGAGAAgg                                                                                                                   >  1:71318/1‑151 (MQ=255)                                  ttACGATAAGCGATCAGCATATTCGTGGGGCACTTAATGTACCCTTGAAAGGGGTAAGGTATGTATTACGCGTGGCGGATGACATTATTGGACCAACGGGTGATGTGATGCCGCTTAATGGACATTATCCCTATACCGAGAAGGTTCACTc                                                                                                            >  1:439740/1‑151 (MQ=255)                                   tACGATAAGCGATCAGCATATTCGTGGGGCACTTAATGTACCCTTGAAAGGGGTAAGGTATGTATTACGCGTGGCGGATGACATTATTGGACCAACGGGTGATGTGATGCCGCTTAATGGACATTATCCCTATACCGAGAAGGTTCACTcc                                                                                                           >  1:448689/1‑151 (MQ=255)                                       aTAAGCGATCAGCATATTCGTGGGGCACTTAATGTACCCTTGAAAGGGGTAAGGTATGTATTACGCGTGGCGGATGACATTATTGGACCAACGGGTGATGTGATGCCGCTTAATGGACATTATCCCTATACCGAGAAGGTTCACTCcaca                                                                                                        >  2:653198/1‑150 (MQ=255)                                       aTAAGCGATCAGCATATTCGTGGGGCACTTAATGTACCCTTGAAAGGGGTAAGGTATGTATTACGCGTGGCGGATGACATTATTGGACCAACGGGTGATGTGATGCCGCTTAATGGACATTATCCCTATACCGAGAAGGTTCACTCcaca                                                                                                        >  2:618785/1‑150 (MQ=255)                                           gCGATCAGCATATTCGTGGGGCACTTAATGTACCCTTGAAAGGGGTAAGGTATGTATTACGCGTGGCGGATGACATTATTGGACCAACGGGTGATGTGATGCCGCTTAATGGACATTATCCCTATACCGAGAAGGTTCACTCCACAAAata                                                                                                   <  1:346034/151‑1 (MQ=255)                                              aTCAGCATATTCGTGGGGCACTTAATGTACCCTTGAAAGGGGTAAGGTATGTATTACGCGTGGCGGATGACATTATTGGACCAACGGGTGATGTGATGCCGCTTAATGGACATTATCCCTATACCGAGAAGGTTCACTCCACAAAATATc                                                                                                 >  1:12766/1‑150 (MQ=255)                                                cAGCATATTCGTGGGGCACTTAATGTACCCTTGAAAGGGGACAGGTATGTATTACGCGTGGCGGATGACATTATTGGACCAACGGGTGATGTGATGCCGCTTAATGGACATTATCCCTATACCGAGAAGGCTCACTCCACAAAATATCAt                                                                                               >  2:108121/1‑150 (MQ=255)                                                   cATATTCGTGGGGCACTTAATGTACCCTTGAAAGGGGTAAGGTATGTATTACGCGTGGCGGATGACATTATTGGACCAACGGGTGATGTGATGCCGCTTAATGGACATTATCCCTATACCGAGAAGGTTCACTCCACAAAATATCATTTc                                                                                            >  2:603635/1‑150 (MQ=255)                                                      atTCGTGGGGCACTTAATGTACCCTTGAAAGGGGTAAGGTATGTATTACGCGTGGCGGATGACATTATTGGACCAACGGGTGATGTGATGCCGCTTAATGGACATTATCCCTATACCGAGAAGGTTCACTCCACAAAATATCATTTCACt                                                                                         <  2:451282/150‑1 (MQ=255)                                                        tCGTGGGGCACTTAATGTACCCTTGAAAGGGGTAAGGTATGTATTACGCGTGGCGGATGACATTATTGGACCAACGGGTGATGTGATGCCGCTTAATGGACATTATCCCTATACCGAGAAGGTTCACTCCACAAAATATCATTTCACtat                                                                                       <  2:191953/150‑1 (MQ=255)                                                         cGTGGGGCACTTAATGTACCCTTGAAAGGGGTAAGGTATGTATTACGCGTGGCGGATGACATTATTGGACCAACGGGTGATGTGATGCCGCTTAATGGACATTATCCCTATACCGAGAAGGTTCACTCCACAAAATATCATTTCACtatt                                                                                      >  1:374365/1‑150 (MQ=255)                                                            ggggCACTTAATGTACCCTTGAAAGGGGTAAGGTATGTATTACGCGTGGCGGATGACATTATTGGACCAACGGGTGATGTGATGCCGCTTAATGGACATTATCCCTATACCGAGAAGGTTCACTCCACAAAATATCATTTCACTATTATCt                                                                                  >  2:865189/1‑151 (MQ=255)                                                             gggCACTTAATGTACCCTTGAAAGGGGTAAGGTATGTATTACGCGTGGCGGATGACATTATTGGACCAACGGGTGATGTGATGCCGCTTAATGGACATTATCCCTATACCGAGAAGGTTCACTCCACAAAATATCATTTCACTATTATCtt                                                                                 >  2:84208/1‑151 (MQ=255)                                                                cACTTAATGTACCCTTGAAAGGGGTAAGGTATGTATTACGCGTGGCGGATGACATTATTGGACCAACGGGTGATGTGATGCCGCTTAATGGACATTATCCCTATACCGAGAAGGTTCACTCCACAAAATATCATTTCACTATTATCTTTaa                                                                              <  1:657469/151‑1 (MQ=255)                                                                      aTGTACCCTTGAAAGGGGTAAGGTATGTATTACGCGTGGCGGATGACATTATTGGACCAACGGGTGATGTGATGCCGCTTAATGGACATTATCCCTATACCGAGAAGGTTCACTCCACAAAATATCATTTCACTATTATCTTTAACccgcc                                                                        >  2:503340/1‑151 (MQ=255)                                                                          aCCCTTGAAAGGGGTAAGGTATGTATTACGCGTGGCGGATGACATTATTGGACCAACGGGTGATGTGATGCCGCTTAATGGACATTATCCCTATACCGAGAAGGTTCACTCCACAAAATATCATTTCACTATTATCTTTAACCCgccgcc                                                                     >  1:895068/1‑150 (MQ=255)                                                                           cccTTGAAAGGGGTAAGGTATGTATTACGCGTGGCGGATGACATTATTGGACCAACGGGTGATGTGATGCCTCTTAATGGACATTATCCCTATCCCGAGAAGGTCCACTCCACAAAATATCATTTCACTATTATCTTTAACccgccg                                                                       <  1:324439/147‑1 (MQ=255)                                                                            ccTTGAAAGGGGTAAGGTATGTATTACGCGTGGCGGATGACATTATTGGACCAACGGGTGATGTGATGCCGCTTAATGGACATTATCCCTATACCGAGAAGGTTCACTCCACAAAATATCATTTCACTATTATCTTTAACCCGCCGCCAct                                                                  <  2:845194/151‑1 (MQ=255)                                                                            ccTTGAAAGGGGTAAGGTATGTATTACGCGTGGCGGATGACATTATTGGACCAACGGGTGATGTGATGCCGCTTAATGGACATTATCCCTATACCGAGAAGGTTCACTCCACAAAATATCATTTCACTATTATCTTTAACCCGCCGCCAct                                                                  <  2:305542/151‑1 (MQ=255)                                                                              ttGAAAGGGGTAAGGTATGTATTACGCGTGGCGGATGACATTATTGGACCAACGGGTGATGTGATGCCGCTTAATGGACATTATCCCTATACCGAGAAGGTTCACTCCACAAAATATCATTTCACTATTATCTTTAACCCGCCGCCActct                                                                >  1:548482/1‑151 (MQ=255)                                                                               tGAAAGGGGTAAGGTATGTATTACGCGTGGCGGATGACATTATTGGACCAACGGGTGATGTGATGCCGCTTAATGGACATTATCCCTATACCGAGAAGGTTCACTCCACAAAATATCATTTCACTATTATCTTTAACCCGCCGCCACTCtt                                                               >  2:840003/1‑151 (MQ=255)                                                                                gAAAGGGGTAAGGTATGTATTACGCGTGGCGGATGACATTATTGGACCAACGGGTGATGTGATGCCGCTTAATGGACATTATCCCTATACCGAGAAGGTTCACTCCACAAAATATCATTTCACTATTATCTTTAACCCGCCGCCACTCttt                                                              >  2:236843/1‑151 (MQ=255)                                                                                 aaaGGGGTAAGGTATTTATTACGCTTGGCGGATGACATTATTGGACCACCGGGTGATGTGATGCCACTTATTGTTCATTATCCCTATCCCGAGAAGTTTCACTCCACAAAATACCATTTCACTATTATCTTTAACCCGCCGCCACTCTTTa                                                             <  1:159192/151‑1 (MQ=255)                                                                                 aaaGGGGTAAGGTATGTATTACGCGTGGCGGATGACATTATTGGACCAACGGGTGATGTGATGCCGCTTAATGGACATTATCCCTATACCGAGAAGGTTCACTCCACAAAATATCATTTCACTATTATCTTTAACCCGCCGCCACTCttt                                                              >  2:19082/1‑150 (MQ=255)                                                                                  aaGGGGTAAGGTATGTATTACGCGTGGCGGATGACATTATTGGACCAACGGGTGATGTGATGCCGCTTAATGGACATTATCCCTATACCGAGAAGGTTCACTCCACAAAATATCATTTCACTATTATCTTTAACCCGCCGCCACTCttt                                                              >  1:442160/1‑149 (MQ=255)                                                                                     gggTAAGGTATGTATTACGCGTGGCGGATGACATTATTGGACCAACGGGTGATGTGATGCCGCTTAATGGACATTATCCCTATACCGAGAAGGTTCACTCCACAAAATATCATTTCACTATTATCTTTAACCCGCCGCCACTCTTTAGCtt                                                         >  2:213877/1‑151 (MQ=255)                                                                                      gttAAGTTATGTATTCCGCGTGGCGTATGACATTATTGTACCAACGGGTGATGTAATGCCTCTTAATGCCCATTTTCCCTATCCGGTGAGGGTTCCCTCCAAAAAATATCATTTCACTATTACCTTTACCCCGCCGCCACTCTTTAGCTTc                                                        <  1:564633/149‑1 (MQ=255)                                                                                         aaGGTATGTATTACGCGTGGCGGATGACATTATTGGCCCAACGGGTGATTTGATGCCGTTTAATGGACATTATCCCTATCCCGAGAAGGTTCCCCCCACAAAATATCATTTCACTATTATCTTTAACCCGCCGCCACTCTTTAGCTTCTAc                                                     <  1:513075/151‑1 (MQ=255)                                                                                            gtatgtatTACGCGTGGCGGATGACATTATTGGACCAACGGGTGATGTGATGCCGCTTAATGGACATTATCCCTATACCGAGAAGGTTCACTCCACAAAATATCATTTCACTATTATCTTTAACCCGCCGCCACTCTTTAGCTTCTAc                                                     >  2:252151/1‑148 (MQ=255)                                                                                               tgtatTACGCGTGGCGGATGACATTATTGGACCAACGGGTGATGTGATGCCGCTTAATGGACATTATCCCTATACCGAGAAGGTTCACTCCACAAAATATCATTTCACTATTATCTTTAACCCGCCGCCACTCTTTAGCTTCTACAGACtt                                               >  1:921402/1‑151 (MQ=255)                                                                                                    tACGCGTGGCGGATGACATTATTGGACCAACGGGTGATGTGATGCCGCTTAATGGACATTATCCCTATACCGAGAAGGTTCACTCCACAAAATATCATTTCACTATTATCTTTACCCCGCCGCCACTCTTTAGCTTCTACAGACTTATCg                                           <  1:207047/150‑1 (MQ=255)                                                                                                    tACGCGTGGCGGATGACATTATTGGACCAACGGGTGATGTGATGCCGCTTAATGGACATTATCCCTATACCGAGAAGGTTCACTCCACAAAATATCATTTCACTATTATCTTTAACCCGCCGCCACTCTTTAGCTTCTACAGACTTATCg                                           >  2:445715/1‑150 (MQ=255)                                                                                                    tACGCGTGGCGGATGACATTATTGGACCAACGGGTGATGTGATGCCGCTTAATGGACATTATCCCTATACCGAGAAGGTTCACTCCACAAAATATCATTTCACTATTATCTTTAACCCGCCGCCACTCTTTAGCTTCTACAGACTTATCg                                           >  2:397776/1‑150 (MQ=255)                                                                                                          tGGCGGATGACATTATTGGACCAACGGGTGATGTGATGCCGCTTAATGGACATTATCCCTATACCGAGAAGGTTCACTCCACAAAATATCATTTCACTATTATCTTTAACCCGCCGCCACTCTTTAGCTTCTACAGACTTATCGATAAAgg                                    >  2:721877/1‑151 (MQ=255)                                                                                                               gATGACATTATTGGACCAACGGGTGATGTGATGCCGCTTAATGGACATTATCCCTATACCGAGAAGGTTCACTCCACAAAATATCATTTCACTATTATCTTTAACCCGCCGCCACTCTTTAGCTTCTACAGACTTATCGATAAAGGCTTTg                               <  2:903585/151‑1 (MQ=255)                                                                                                                      ttattGGACCAACGGGTGATGTGATGCCGCTTAATGGACATTATCCCTATCCCGAGAAGTTTCACTCCACAAAATATCATTCCACTATTATCTTTACCCCGCCGCCACTCTTTAGCTTCTACAGACTTATCGATAAAGGCTTTGGGAtt                          <  1:838644/149‑1 (MQ=255)                                                                                                                      ttattGGACCAACGGGTGATGTGATGCCGCTTAATGGACATTATCCCTATACCGAGAAGGTTCACTCCACAAAATATCATTTCACTATTATCTTTAACCCGCCGCCACTCTTTAGCTTCTACAGACTTATCGATAAAGGCTTTGGGAtttt                        >  2:722434/1‑151 (MQ=255)                                                                                                                      ttattGGACCAACGGGTGATGTGATGCCGCTTAATGGACATTATCCCTATACCGAGAAGGTTCACTCCACAAAATATCATTTCACTATTATCTTAAACCCGCCGCCACTCTTTAGCTTCTACAGACTTATCGATAAAGGCTTTGGGAttt                         <  1:315155/150‑1 (MQ=255)                                                                                                                       tattGGACCAACGGGTGATGTGATGCCGCTTAATGGACATTATCCCTATACCGAGAAGGTTCACTCCACAAAATATCATTTCACTATTATCTTTAACCCGCCGCCACTCTTTAGCTTCTACAGACTTATCGATAAAGGCTTTGGGAtttt                        <  1:730617/150‑1 (MQ=255)                                                                                                                          tGGACCAACGGGTGATGTGATGCCGCTTAATGGACATTATCCCTATACCGAGAAGGTTCACTCCACAAAATATCATTTCACTATTATCTTTAACCCGCCGCCACTCTTTAGCTTCTACAGACTTATCGATAAAGGCTTTGGGATTTTAat                     >  1:576673/1‑150 (MQ=255)                                                                                                                           ggACCAACGGGTGATGTGATGCCGCTTAATGGACATTATCCCTATACCGAGAAGGTTCACTCCACAAAATATCATTTCACTATTATCTTTAACCCGCCGCCACTCTTTAGCTTCTACAGACTTATCGATAAAGGCTTTGGGATTTTAatat                   >  2:488137/1‑151 (MQ=255)                                                                                                                             aCCAACGGGTGATGTGATGCCGCTTAATGGACATTATCCCTATACCGAGAAGGTTCACTCCACAAAATATCATTTCACTATTATCTTTAACCCGCCGCCACTCTTTAGCTTCTACAGACTTATCGATAAAGGCTTTGGGATTTTAAtattt                 >  1:9840/1‑151 (MQ=255)                                                                                                                              ccAACGGGTGATGTGATGCCGCTTAATGGACATTATCCCTATACCGAGAAGGTTCACTCCACAAAATATCATTTCACTATTATCTTTAACCCGCCGCCACTCTTTAGCTTCTACAGACTTATCGATAAAGGCTTTGGGATTTTAAtattt                 >  2:628027/1‑150 (MQ=255)                                                                                                                                    ggTGAAGTGCTGCTGTTTAATCGCCATTATCCCTCTCCCGTTACCGTTCTCCCCTCAAAATTTCATTGCCCTTTTATCTTTATCGCCGCGCCACTCTTTAGCTTCTACAGACTTATCGATAAAGGCATTGGGATTTTAAtattta                <  1:485610/145‑1 (MQ=255)                                                                                                                                       gatgtgatgCCGCTTAATGGACATTATCCCTATACCGAGAAGGTTCACTCCACAAAATATCATTTCACTATTATCTTTAACCCGCCGCCACTCTTTAGCTTCTACAGACTTATCGATAAAGGCTTTGGGATTTTAATATTTATTCTGTTa        <  2:25584/150‑1 (MQ=255)                                                                                                                                         tgtgatgCCGCTTAATGGACATTATCCCTATACCGAGAAGGTTCACTCCACAAAATATCATTTCACTATTATCTTTAACCCGCCGCCACTCTTTAGCTTCTACAGACTTATCGATAAAGGCTTTGGGATTTTAATATTTATTCTGTTAAt      <  1:642738/150‑1 (MQ=255)                                                                                                                                         tgtgatgCCGCTTAATGGACATTATCCCTATACCGAGAAGGTTCACTCCACAAAATATCATTTCACTATTATCTTTAACCCGCCGCCACTCTTTAGCTTCTACAGACTTATCGATAAAGGCTTTGGGATTTTAATATTTATTCTGTTAATc     >  1:905546/1‑151 (MQ=255)                                                                                                                                          gtgatgCCGCTTAATGGACATTATCCCTATACCGAGAAGTTTCACTCCACAAAATATCATTTCACTATTATCTTTAACCCGCCGCCACTCTTTAGCTTCTACAGACTTATCGATAAAGGCTTTGGGATTTTAATATTTATTCTGTTAAt      <  1:556401/149‑1 (MQ=255)                                                                                                                                            gatgcCGCTTAATGGACATTATCCCTATACCGAGAAGGTTCACTCCACAAAATATCATTTCACTATTATCTTTAACCCGCCGCCACTCTTTAGCTTCTACAGACTTATCGATAAAGGCTTTGGGATTTTAATATTTATTCTGTTAATCGcc  >  2:935298/1‑151 (MQ=255)                                                                                                                                                |                                                                                                                                                   ATCAGAACCAATTTGCAGATACGCGCATTTTGGTTACGATAAGCGATCAGCATATTCGTGGGGCACTTAATGTACCCTTGAAAGGGGTAAGGTATGTATTACGCGTGGCGGATGACATTATTGGACCAACGGGTGATGTGATGACGCTTAATGGACATTATCCCTATACCGAGAAGGTTCACTCCACAAAATATCATTTCACTATTATCTTTAACCCGCCGCCACTCTTTAGCTTCTACAGACTTATCGATAAAGGCTTTGGGATTTTAATATTTATTCTGTTAATCGCC  >  NC_000913/1217734‑1218023

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑