Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,265,930	A→C	N307H (AAC→CAC)	prfA →	peptide chain release factor RF‑1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,265,930	0	A	C	100.0%	166.0 / NA	48	N307H (AAC→CAC)	prfA	peptide chain release factor RF‑1
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (27/21);  total (27/21)

CGAACGTTCACAACATAAAAACAAAGCTAAAGCACTTTCTGTTCTCGGTGCTCGCATCCACGCTGCTGAAATGGCAAAACGCCAACAGGCCGAAGCGTCTACCCGTCGTAACCTGCTGGGGAGTGGCGATCGCAGCGACCGTAACCGTACTTACAACTTCCCGCAGGGGCGCGTTACCGATCACCGCATCAACCTGACGCTCTACCGCCTGGATGAAGTGATGGAAGGTAAGCTGGATATGCTGATTGAACCGATTATCCAGGAACATCAGGCCGACCAACTGGCGGCG  >  NC_000913/1265788‑1266076                                                                                                                                               |                                                                                                                                                   cGAACGTTCACAACATAAAAACAAAGCTAAAGCACTTTCTGTTCTCGGTGCTCGCATCCACGCTGCTGAAATGGCAAAACGCCAACAGGCCGAAGCGTCTACCCGTCGTAACCTGCTGGGGAGTGGCGATCGCAGCGACCGTCACCGTACt                                                                                                                                            >  1:217602/1‑151 (MQ=255)  gAACGTTCACAACATAAAAACAAAGCTAAAGCACTTTCTGTTCTCGGTGCTCGCATCCACGCTGCTGAAATGGCAAAACGCCAACAGGCCGAAGCGTCTACCCGTCGTAACCTGCTGGGGAGTGGCGATCGCAGCGACCGTCACCGTACtt                                                                                                                                           >  2:326379/1‑151 (MQ=255)  gAACGTTCACAACATAAAAACAAAGCTAAAGCACTTTCTGTTCTCGGTGCTCGCATCCACGCTGCTGAAATGGCAAAACGCCAACAGGCCGAAGCGTCTACCCGTCGTAACCTGCTGGGGAGTGGCGATCGCAGCGACCGTCACCGTACtt                                                                                                                                           <  2:117056/151‑1 (MQ=255)   aaCGTTCACAACATAAAAACAAAGCTAAAGCACTTTCTGTTCTCGGTGCTCGCATCCACGCTGCTGAAATGGCAAAACGCCAACAGGCCGAAGCGTCTACCCGTCGTAACCTGCTGGGGAGTGGCGATCGCAGCGACCGTCACCGTACtt                                                                                                                                           >  2:825372/1‑150 (MQ=255)      gTTCACAACATAAAAACAAAGCTAAAGCACTTTCTGTTCTCGGTGCTCGCATCCACGCTGCTGAAATGGCAAAACGCCAACAGGCCGAAGCGTCTACCCGTCGTAACCTGCTGGGGAGTGGCGATCGCAGCGACCGTCACCGTACTTAc                                                                                                                                         >  1:117503/1‑149 (MQ=255)             acaTAAAAACAAAGCTAAAGCACTTTCTGTTCTCGGTGCTCGCATCCACGCTGCTGAAATGGCAAAACGCCAACAGGCCGAAGCGTCTACCCGTCGTAACCTGCTGGGGAGTGGCGATCGCAGCGACCGTCACCGTACTTACAACTTcc                                                                                                                                  <  1:443503/149‑1 (MQ=255)                tAAAAACAAAGCTAAAGCACTTTCTGTTCTCGGTGCTCGCATCCACGCTGCTGAAATGGCAAAACGCCAACAGGCCGAAGCGTCTACCCGTCGTAACCTGCTGGGGAGTGGCGATCGCAGCGACCGTCACCGTACTTACAACTTCCCGc                                                                                                                               >  1:20338/1‑149 (MQ=255)                 aaaaaCAAAGCTAAAGCACTTTCTGTTCTCGGTGCTCGCATCCACGCTGCTGAAATGGCAAAACGCCAACAGGCCGAAGCGTCTACCCGTCGTAACCTGCTGGGGAGTGGCGATCGCAGCGACCGTCACCGTACTTACAACTTCCCGCAgg                                                                                                                            >  1:344406/1‑151 (MQ=255)                 aaaaaCAAAGCTAAAGCACTTTCTGTTCTCGGTGCTCGCATCCACGCTGCTGAAATGGCAAAACGCCAACAGGCCGAAGCGTCTACCCGTCGTAACCTGCTGGGGAGTGGCGATCGCAGCGACCGTCACCGTACTTACAACTTCCCGCAgg                                                                                                                            >  1:62022/1‑151 (MQ=255)                  aaaaCAAAGCTAAAGCACTTTCTGTTCTCGGTGCTCGCATCCACGCTGCTGAAATGGCAAAACGCCAACAGGCCGAAGCGTCTACCCGTCGTAACCTGCTGGGGAGTGGCGATCGCAGCGACCGTCACCGTACTTACAACTTCCCGCAggg                                                                                                                           <  2:506473/151‑1 (MQ=255)                    aaCAAAGCTAAAGCACTTTCTGTTCTCGGTGCTCGCATCCACGCTGCTGAAATGGCAAAACGCCAACAGGCCGAAGCGTCTACCCGTCGTAACCTGCTGGGGAGTGGCGATCGCAGCGACCGTCACCGTACTTACAACTTCCCGCAGGGgc                                                                                                                         <  2:836115/151‑1 (MQ=255)                    aaCAAAGCTAAAGCACTTTCTGTTCTCGGTGCTCGCATCCACGCTGCTGAAATGGCAAAACGCCAACAGGCCGAAGCGTCTACCCGTCGTAACCTGCTGGGGAGTGGCGATCGCAGCGACCGTCACCGTACTTACAACTTCCCGCAGGGgc                                                                                                                         >  1:846420/1‑151 (MQ=255)                        aaGCTAAAGCACTTTCTGTTCTCGGTGCTCGCATCCACGCTGCTGAAATGGCAAAACGCCAACAGGCCGAAGCGTCTACCCGTCGTAACCTGCTGGGGAGTGGCGATCGCAGCGACCGTCACCGTACTTACAACTTCCCGCAGGGGCGCGt                                                                                                                     >  2:17313/1‑151 (MQ=255)                            tAAAGCACTTTCTGTTCTCGGTGCTCGCATCCACGCTGCTGAAATGGCAAAACGCCAACAGGCCGAAGCGTCTACCCGTCGTAACCTGCTGGGGAGTGGCGATCGCAGCGACCGTCACCGTACTTACAACTTCCCGCAGGGGCGCGTTAcc                                                                                                                 >  2:746386/1‑151 (MQ=255)                            tAAAGCACTTTCTGTTCTCGGTGCTCGCATCCACGCTGCTGAAATGACAAAACGCCAACAGGCCGAAGCGTCTACCCGTCGTAACCTGCTGGGGAGTGGCGATCGCAGCGACCGTCACCGTACTTACAACTTCCCGCAAGGGCGCGTTAcc                                                                                                                 >  2:589130/1‑151 (MQ=255)                               aGCACTTTCTGTTCTCGGTGCTCGCATCCACGCTGCTGAAATGGCAAAACGCCAACAGGCCGAAGCGTCTACCCGTCGTAACCTGCTGGGGAGTGGCGATCGCAGCGACCGTCACCGTACTTACAACTTCCCGCAGGGGCGCGTTACCGAt                                                                                                              <  2:896149/151‑1 (MQ=255)                                   cTTTCTGTTCTCGGTGCTCGCATCCACGCTGCTGAAATGGCAAAACGCCAACAGGCCGAAGCGTCTACCCGTCGTAACCTGCTGGGGAGTGGCGATCGCAGCGACCGTCACCGTACTTACAACTTCCCGCAGGGGCGCGTTACCGATCAcc                                                                                                          >  2:84799/1‑151 (MQ=255)                                      tCTGTTCTCGGTGCTCGCATCCACGCTGCTGAAATGGCAAAACGCCAACAGGCCGAAGCGTCTACCCGTCGTAACCTGCTGGGGAGTGGCGATCGCAGCGACCGTCACCGTACTTACAACTTCCCGCAGGGGCGCGTTACCGATCACCGc                                                                                                        >  1:39227/1‑150 (MQ=255)                                           tctcGGTGCTCGCATCCACGCTGCTGAAATGGCAAAACGCCAACAGGCCGAAGCGTCTACCCGTCGTAACCTGCTGGGGAGTGGCGATCGCAGCGACCGTCACCGTACTTACAACTTCCCGCAGGGGCGCGTTACCGATCACCGCATCAAc                                                                                                  >  2:132645/1‑151 (MQ=255)                                           tctcGGTGCTCGCATCCACGCTGCTGAAATGGCAAAACGCCAACAGGCCGAAGCGTCTACCCGTCGTAACCTGCTGGGGAGTGGCGATCGCAGCGACCGTCACCGTACTTACAACTTCCCGCAGGGGCGCGTTACCGATCACCGCATCAAc                                                                                                  >  2:372835/1‑151 (MQ=255)                                              cGGTGCTCGCATCCACGCTGCTGAAATGGCAAAACGCCAACAGGCCGAAGCGTCTACCCGTCGTAACCTGCTGGGGAGTGGCGATCGCAGCGACCGTCACCGTACTTACAACTTCCCGCAGGGGCGCGTTACCGATCACCGCATCAACCt                                                                                                <  1:616307/150‑1 (MQ=255)                                              cGGTGCTCGCATCCACGCTGCTGAAATGGCAAAACGCCAACAGGCCGAAGCGTCTACCCGTCGTAACCTGCTGGGGAGTGGCGATCGCAGCGACCGTCACCGTACTTACAACTTCCCGCAGGGGCGCGTTACCGATCACCGCATCAACCTg                                                                                               >  1:569335/1‑151 (MQ=255)                                                gTGCTCGCATCCACGCTGCTGAAATGGCAAAACGCCAACAGGCCGAAGCGTCTACCCGTCGTAACCTGCTGGGGAGTGGCGATCGCAGCGACCGTCACCGTACTTACAACTTCCCGCAGGGGCGCGTTACCGATCACCGCATCAACCTGAc                                                                                             <  1:221111/151‑1 (MQ=255)                                                 tGCTCGCATCCACGCTGCTGAAATGGCAAAACGCCAACAGGCCGAAGCGTCTACCCGTCGTAACCTGCTGGGGAGTGGCGATCGCAGCGACCGTCACCGTACTTACAACTTCCCGCAGGGGCGCGTTACCGATCACCGCATCAACCTGAc                                                                                             >  1:827414/1‑150 (MQ=255)                                                   cTCGCATCCACGCTGCTGAAATGGCAAAACGCCAACAGGCCGAAGCGTCTACCCGTCGTAACCTGCTGGGGAGTGGCGATCGCAGCGACCGTCACCGTACTTACAACTTCCCGCAGGGGCGCGTTACCGATCACCGCATCAACCTGACGc                                                                                           >  1:814273/1‑150 (MQ=255)                                                     cGCATCCACGCTGCTGAAATGGCAAAACGCCAACAGGCCGAAGCGTCTACCCGTCGTAACCTGCTGGGGAGTGGCGATCGCAGCGACCGTCACCGTACTTACAACTTCCCGCAGGGGCGCGTTACCGATCACCGCATCAACCTGACGctc                                                                                         <  1:468950/150‑1 (MQ=255)                                                        aTCCACGCTGCTGAAATGGCAAAACGCCAACAGGCCGAAGCGTCTACCCGTCGTAACCTGCTGGGGAGTGGCGATCGCAGCGACCGTCACCGTACTTACAACTTCCCGCAGGGGCGCGTTACCGATCACCGCATCAACCTGACGCTCTAcc                                                                                     <  2:61195/151‑1 (MQ=255)                                                             cGCTGCTGAAATGGCAAAACGCCAACAGGCCGAAGCGTCTACCCGTCGTAACCTGCTGGGGAGTGGCGATCGCAGCGACCGTCACCGTACTTACAACTTCCCGCAGGGGCGCGTTACCGATCACCGCATCAACCTGACGCTCTACCGCCTg                                                                                <  1:596398/151‑1 (MQ=255)                                                                   tgAAATGGCAAAACGCCAACAGGCCGAAGCGTCTACCCGTCGTAACCTGCTGGGGAGTGGCGATCGCAGCGACCGTCACCGTACTTACAACTTCCCGCAGGGGCGCGTTACCGATCACCGCATCAACCTGACGCTCTACCGCCTGGATGa                                                                           >  2:403831/1‑150 (MQ=255)                                                                     aaaTGGCAAAACGCCAACAGGCCGAAGCGTCTACCCGTCGTAACCTGCTGGGGAGTGGCGATCGCAGCGACCGTCACCGTACTTACAACTTCCCGCAGGGGCGCGTTACCGATCACCGCATCAACCTGACGCTCTACCGCCTGGATGAAGt                                                                        <  2:445458/151‑1 (MQ=255)                                                                      aaTGGCAAAACGCCAACAGGCCGAAGCGTCTACCCGTCGTAACCTGCTGGGGAGTGGCGATCGCAGCGACCGTCACCGTACTTACAACTTCCCGCAGGGGCGCGTTACCGATCACCGCATCAACCTGACGCTCTACCGCCTGGATGAAGTg                                                                       <  1:439256/151‑1 (MQ=255)                                                                            aaaaCGCCAACAGGCCGAAGCGTCTCCCCGTCGTAACCTGCTGGGGAGTGGCGATCGCAGCGACCGTCACCGTACTTACAACTTCCCGCAGGGGCGCGTTACCGATCACCGCATCAACCTGACGCTCTACCGCCTGGATGAAGTGATGGaa                                                                 <  2:117505/151‑1 (MQ=255)                                                                              aaCGCCAACAGGCCGAAGCGTCTACCCGTCGTAACCTGCTGGGGAGTGGCGATCGCAGCGACCGTCACCGTACTTACAACTTCCCGCAGGGGCGCGTTACCGATCACCGCATCAACCTGACGCTCTACCGCCTGGATGAAGTGATGGAAgg                                                               >  1:698821/1‑151 (MQ=255)                                                                                       aGGCCGAAGCGTCTACCCGTCGTAACCTGCTGGGGAGTGGCGATCGCAGCGACCGTCACCGTACTTACAACTTCCCGCAGGGGCGCGTTACCGATCACCGCATCAACCTGACGCTCTACCGCCTGGATGAAGTGATGGAAGGTAAGCTGGa                                                      <  2:39229/151‑1 (MQ=255)                                                                                           cGAAGCGTCTACCCGTCGTAACCTGCTGGGGAGTGGCGATCGCAGCGACCGTCACCGTACTTACAACTTCCCGCAGGGGCGCGTTACCGATCACCGCATCAACCTGACGCTCTACCGCCTGGATGAAGTGATGGAAGGTAAGCTGGATATg                                                  >  1:688858/1‑151 (MQ=255)                                                                                                cGTCTCCCCGTCGTAACCTGCTGGGGAGTGGCGATCGCAGCGTCCGTCACCGTACTTACAACTTCCCGCAGGGGCGCGTTACCGATCACCGCATCAACCTGACGCTCTACCGCCTGGATGAAGTGATGGAAGGTAAGCTGGATATGCTGAt                                             <  2:310982/151‑1 (MQ=255)                                                                                                 gTCTACCCGTCGTAACCTGCTGGGGAGTGGCGATCGCAGCGACCGTCACCGTACTTACAACTTCCCGCAGGGGCGCGTTACCGATCACCGCATCAACCTGACGCTCTACCGCCTGGATGAAGTGATGGAAGGTAAGCTGGATATGCTGAtt                                            >  2:610218/1‑151 (MQ=255)                                                                                                    tACCCGTCGTAACCTGCTGGGGAGTGGCGATCGCAGCGACCGTCACCGTACTTACAACTTCCCGCAGGGGCGCGTTACCGATCACCGCATCAACCTGACGCTCTACCGCCTGGATGAAGTGATGGAAGGTAAGCTGGATATGCTGATTGa                                          >  1:794635/1‑150 (MQ=255)                                                                                                      cccGTCGTAACCTGCTGGGGAGTGGCGATCGCAGCGACCGTCACCGTACTTACAACTTCCCGCAGGGGCGCGTTACCGATCACCGCATCAACCTGACGCTCTACCGCCTGGATGAAGTGATGGAAGGTAAGCTGGATATGCTGATTGAAcc                                       <  2:890788/151‑1 (MQ=255)                                                                                                                  tgctgGGGAGTGGCGATCGCAGCGACCGTCACCGTACTTACAACTTCCCGCAGGGGCGCGTTACCGATCACCGCATCAACCTGACGCTCTACCGCCTGGATGAAGTGATGGAAGGTAAGCTGGATATGCTGATTGAACCGATTATCCAgg                            >  1:406391/1‑150 (MQ=255)                                                                                                                   gctgGGGAGTGGCGATCGCAGCGACCGTCACCGTACTTACAACTTCCCGCAGGGGCGCGTTACCGATCACCGCATCAACCTGACGCTCTACCGCCTGGATGAAGTGATGGAAGGTAAGCTGGATATGCTGATTGAACCGATTATCCAGGaa                          <  1:328214/151‑1 (MQ=255)                                                                                                                       gggAGTGGCGATCGCAGCGACCGTCACCGTACTTACAACTTCCCGCAGGGGCGCGTTACCGATCACCGCATCAACCTGACGCTCTACCGCCTGGATGAAGTGATGGAAGGTAAGCTGGATATGCTGATTGAACCGATTATCCAGGAAc                         <  1:41873/148‑1 (MQ=255)                                                                                                                              gCGATCGCAGCGACCGTCACCGTACTTACAACTTCCCGCAGGGGCGCGTTACCGATCACCGCATCAACCTGACGCTCTACCGCCTGGATGAAGTGATGGAAGGTAAGCTGGATATGCTGATTGAACCGATTATCCAGGAACATCAGGCCGa               <  1:768030/151‑1 (MQ=255)                                                                                                                              gCGATCGCAGCGACCGTCACCGTACTTACAACTTCCCGCAGGGGCGCGTTACCGATCACCGCATCAACCTGACGCTCTACCGCCTGGATGAAGTGATGGAAGGTAAGCTGGATATGCTGATTGAACCGATTATCCAGGAACATCAGGCCGa               <  1:200527/151‑1 (MQ=255)                                                                                                                               cGATCGCAGCGACCGTCACCGTACTTACAACTTCCCGCAGGGGCGCGTTACCGATCACCGCATCAACCTGACGCTCTACCGCCTGGATGAAGTGATGGAAGGTAAGCTGGATATGCTGATTGAACCGATTATCCAGGAACATCAGGCCGAc              >  2:655931/1‑151 (MQ=255)                                                                                                                                 aTCGCAGCGACCGTCACCGTACTTACAACTTCCCGCAGGGGCGCGTTACCGATCACCGCATCAACCTGACGCTCTACCGCCTGGATGAAGTGATGGAAGGTAAGCTGGATATGCTGATTGAACCGATTATCCAGGAACATCAGGCCGACCa            <  2:62024/151‑1 (MQ=255)                                                                                                                                    gCAGCGACCGTCACCGTACTTACAACTTCCCGCAGGGGCGCGTTACCGATCACCGCATCAACCTGACGCTCTACCGCCTGGATGAAGTGATGGAAGGTAAGCTGGATATGCTGATTGAACCGATTATCCAGGAACATCAGGCCGACCAACt         >  1:721129/1‑151 (MQ=255)                                                                                                                                       gCGACCGTCACCGTACTTACAACTTCCCGCAGGGGCGCGTTACCGATCACCGCATCAACCTGACGCTCTACCGCCTGGATGAAGTGATGGAAGGTAAGCTGGATATGCTGATTGAACCGATTATCCAGGAACATCAGGCCGACCAACTggc      >  2:908152/1‑151 (MQ=255)                                                                                                                                          accgtcaccgtaCTTACAACTTCCCGCAGGGGCGCGTTACCGATCACCGGATCAACCAGACGCTCTACCGCCTGGATGAAGTGATGGAAGGTAAGCTGGATATGCTGATTGAACCGATTATCCAGGAACATCAGGCCGACCAACTggcgg    >  1:402589/1‑150 (MQ=255)                                                                                                                                          accgtcaccgtaCTTACAACTTCCCGCAGGGGCGCGTTACCGATCACCGCATCAACCTGACGCTCTACCGCCTGGATGAAGTGATGGAAGGTAAGCTGGATATGCTGATTGAACCGATTATCCAGGAACATCAGGCCGACCAACTggcggc   >  1:195180/1‑151 (MQ=255)                                                                                                                                          accgtcaccgtaCTTACAACTTCCCGCAGGGGCGCGTTACCGATCACCGCATCAACCTGACGCTCTACCGCCTGGATGAAGTGATGGAAGGTAAGCTGGATATGCTGATTGAACCGATTATCCAGGAACATCAGGCCGACCAACTggcgg    >  1:350595/1‑150 (MQ=255)                                                                                                                                           ccgtcaccgtaCTTACAACTTCCCGCAGGGGCGCGTTACCGATCACCGCATCAACCTGACGCTCTACCGCCTGGATGAAGTGATGGAAGGTAAGCTGGATATGCTGATTGAACCGATTATCCAGGAACATCAGGCCGACCAACTggcggct  <  1:589111/151‑2 (MQ=255)                                                                                                                                               |                                                                                                                                                   CGAACGTTCACAACATAAAAACAAAGCTAAAGCACTTTCTGTTCTCGGTGCTCGCATCCACGCTGCTGAAATGGCAAAACGCCAACAGGCCGAAGCGTCTACCCGTCGTAACCTGCTGGGGAGTGGCGATCGCAGCGACCGTAACCGTACTTACAACTTCCCGCAGGGGCGCGTTACCGATCACCGCATCAACCTGACGCTCTACCGCCTGGATGAAGTGATGGAAGGTAAGCTGGATATGCTGATTGAACCGATTATCCAGGAACATCAGGCCGACCAACTGGCGGCG  >  NC_000913/1265788‑1266076

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑