Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,277,396	T→G	K75Q (AAA→CAA)	narX ←	sensory histidine kinase in two‑component regulatory system with NarL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,277,396	0	T	G	100.0%	195.7 / NA	52	K75Q (AAA→CAA)	narX	sensory histidine kinase in two‑component regulatory system with NarL
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (29/23);  total (29/23)

ACCGTTTCTCGGTTTTGTGCACGCATCAGCGCAGGGATCAGTTCATTACGCCAGTAATCTTGTAAACCCTGTAATTGCGCCAGTTGTCCGTCTCGTTCTGCTGCTCGAGTCAACTCGGCGCTAAATGCCGTTTGTTCCATCTCTTTAATTAAGGGCTTGTCTTTCTCGCTTAATGGCACTGCCGCCAACAGACGGTAACTTTGCATGCGCAGCGATCCCGCTTTGTTGATCGCATGGGCGCTGCCCTGAACGCCTTGCACCAGCCAGCCAGAAACCGCCATCC  >  NC_000913/1277251‑1277533                                                                                                                                                  |                                                                                                                                          acCGTTTCTCGGTTTTGTGCACGCATCAGCGCAGGGATCAGTTCATTACGCCAGTAATCTTGTAAACCCTGTAATTGCGCCAGTTGTCCGTCTCGTTCTGCTGCTCGAGTCAACTCGGCGCTAAATGCCGTTTGTTCCATCTCTtgaatta                                                                                                                                      <  2:127195/151‑1 (MQ=255)   cGTTTCTCGGTTTTGTGCACGCATCAGCGCAGGTATCAGTTCATTACGCCAGTAATCTTGTAAACCCTGTAATTGCGCCAGTTGTCCGTCTCGTTCTGCTGCTCGAGTCAACTCGGCGCTAAATGCCGTTTGTTCCATCTCTTGAATTAAg                                                                                                                                    <  2:104361/151‑1 (MQ=255)   cGTTTCTCGGTTTTGTGCACGCATCAGCGCAGGGATCAGTTCATTACGCCAGTAATCTTGTAAACCCTGTAATTGCGCCAGTTGTCCGTCTCGTTCTGCTGCTCGAGTCAACTCGGCGCTAAATGCCGTTTGTTCCATCTCTtgaatta                                                                                                                                      >  2:169162/1‑149 (MQ=255)    gTTTCTCGGTTTTGTGCACGCATCAGCGCAGGGATCAGTTCATTACGCCAGTAATCTTGTAAACCCTGTAATTGCGCCAGTTGTCCGTCTCGTTCTGCTGCTCGAGTCAACTCGGCGCTAAATGCCGTTTGTTCCATCTCTTGAATTAAgg                                                                                                                                   <  1:900130/151‑1 (MQ=255)       tctcGGTTTTGTGCACGCATCAGCGCAGGGATCAGTTCATTACGCCAGTAATCTTGTAAACCCTGTAATTGCGCCAGTTGTCCGTCTCGTTCTGCTGCTCGAGTCAACTCGGCGCTAAATGCCGTTTGTTCCATCTCTTGAATTAAGGGc                                                                                                                                 <  1:221302/150‑1 (MQ=255)          cGGTTTTGTGCACGCATCAGCGCAGGGATCAGTTCATTACGCCAGTAATCTTGTAAACCCTGTAATTGCGCCAGTTGTCCGTCTCGTTCTGCTGCTCGAGTCAACTCGGCGCTAAATGCCGTTTGTTCCATCTCTTGAATTAAGGGCTTg                                                                                                                              <  1:585104/150‑1 (MQ=255)               ttGTGCACGCATCAGCGCAGGGATCAGTTCATTACGCCAGTAATCTTGTAAACCCTGTAATTGCGCCAGTTGTCCGTCTCGTTCTGCTGCTCGAGTCAACTCGGCGCTAAATGCCGTTTGTTCCATCTCTTGAATTAAGGGCTTGTCTTtc                                                                                                                        >  2:404938/1‑151 (MQ=255)                 gtgCACGCATCAGCGCAGGGATCAGTTCATTACGCCAGTAATCTTGTAAACCCTGTAATTGCGCCAGTTGTCCGTCTCGTTCTGCTGCTCGAGTCAACTCGGCGCTAAATGCCGTTTGTTCCATCTCTTGAATTAAGGGCTTGTCTTtctc                                                                                                                      <  2:904299/151‑1 (MQ=255)                   gCACGCATCAGCGCAGGGATCAGTTCATTACGCCAGTAATCTTGTAAACCCTGTAATTGCGCCAGTTGTCCGTCTCGTTCTGCTGCTCGAGTCAACTCGGCGCTAAATGCCGTTTGTTCCATCTCTTGAATTAAGGGCTTGTCTTTCTCGc                                                                                                                    >  1:680054/1‑151 (MQ=255)                           cAGCGCAGGGATCAGTTCATTACGCCAGTAATCTTGTAAACCCTGTAATTGCGCCAGTTGTCCGTCTCGTTCTGCTGCTCGAGTCAACTCGGCGCTAAATGCCGTTTGTTCCATCTCTTGAATTAAGGGCTTGTCTTTCTCGCTTAATgg                                                                                                             <  2:877748/150‑1 (MQ=255)                                  gggATCAGTTCATTACGCCAGTAATCTTGTAAACCCTGTAATTGCGCCAGTTGTCCGTCTCGTTCTGCTGCTCGAGTCAACTCGGCGCTAAATGCCGTTTGTTCCATCTCTTGAATTAAGGGCTTGTCTTTCTCGCTTAATGGCACTgccg                                                                                                     >  1:919105/1‑151 (MQ=255)                                     aTCAGTTCATTACGCCAGTAATCTTGTAAACCCTGTAATTGCGCCAGTTGTCCGTCTCGTTCTGCTGCTCGAGTCAACTCGGCGCTAAATGCCGTTTGTTCCATCTCTTGAATTAAGGGCTTGTCTTTCTCGCTTAATGGCACTGCCGCCa                                                                                                  <  1:197851/151‑1 (MQ=255)                                          ttCATTACGCCAGTAATCTTGTAAACCCTGTAATTGCGCCAGTTGTCCGTCTCGTTCTGCTGCTCGAGTCAACTCGGCGCTAAATGCCGTTTGTTCCATCTCTTGAATTAAGGGCTTGTCTTTCTCGCTTAATGGCACTGCCGCCAAc                                                                                                >  2:619747/1‑148 (MQ=255)                                            cATTACGCCAGTAATCTTGTAAACCCTGTAATTGCGCCAGTTGTCCGTCTCGTTCTGCTGCTCGAGTCAACTCGGCGCTAAATGCCGTTTGTTCCATCTCTTGAATTAAGGGCTTGTCTTTCTCGCTTAATGGCACTGCCGCCAACAGAc                                                                                            <  2:236190/150‑1 (MQ=255)                                            cATTACGCCAGTAATCTTGTAAACCCTGTAATTGCGCCAGTTGTCCGTCTCGTTCTGCTGCTCGAGTCAACTCGGCGCTAAATGCCGTTTGTTCCATCTCTTGAATTAAGGGCTTGTCTTTCTCGCTTAATGGCACTGCCGCCAACAGACg                                                                                           <  2:672571/151‑1 (MQ=255)                                             aTTACGCCAGTAATCTTGTAAACCCTGTAATTGCGCCAGTTGTCCGTCTCGTTCTGCTGCTCGAGTCAACTCGGCGCTAAATGCCGTTTGTTCCATCTCTTGAATTAAGGGCTTGTCTTTCTCGCTTAATGGCACTGCCGCCAACAGACgg                                                                                          >  2:787417/1‑151 (MQ=255)                                                aCGCCAGTAATCTTGTAAACCCTGTAATTGCGCCAGTTGTCCGTCTCGTTCTGCTGCTCGAGTCAACTCGGCGCTAAATGCCGTTTGTTCCATCTCTTGAATTAAGGGCTTGTCTTTCTCGCTTAATGGCACTGCCGCCAACAGACGGTaa                                                                                       <  2:44640/151‑1 (MQ=255)                                                   ccAGCAATCTTGTAACCCCTGTAATTGCGACAGTTGTCCGTCTCGTTGTGCTGCTCGAGTCAACTCGGCGCTAAATGCCGTCTGTTTCGTCTCTTGAATTAAGGGCTTGTCTTTCTCGCTTAATGGCACTACCGCCAACAGACGGTTACtt                                                                                    >  1:862251/1‑151 (MQ=255)                                                    cAGTAATCTTGTAAACCCTGTAATTGCGCCAGTTGTCCGTCTCGTTCTGCTGCTCGAGTCAACTCGGCGCTAAATGCCGTTTGTTCCATCTCTTGAATTAAGGGCTTGTCTTTCTCGCTTAATGGCACTGCCGCCAACAGACGGTAACttt                                                                                   >  1:721421/1‑151 (MQ=255)                                                     aGTAATCTTGTAAACCCTGTAATTGCGCCAGTTGTCCGTCTCGTTCTGCTGCTCGAGTCAACTCGGCGCTAAATGCCGTTTGTTCCATCTCTTGAATTAAGGGCTTGTCTTTCTCGCTTAATGGCACTGCCGCCAACAGACGGTAACTTTg                                                                                  >  2:485063/1‑151 (MQ=255)                                                      gTAATCTTGTAAACCCTGTAATTGCGCCAGTTGTCCGTCTCGTTCTGCTGCTCGAGTCAACTCGGCGCTAAATGCCGTTTGTTCCATCTCTTGAATTAAGGGCTTGTCTTTCTCGCTTAATGGCACTGCCGCCAACAGACGGTAACTTTGc                                                                                 <  2:606416/151‑1 (MQ=255)                                                        aaTCTTGTAAACCCTGTAATTGCGCCAGTTGTCCGTCTCGTTCTGCTGCTCGAGTCAACTCGGCGCTAAATGCCGTTTGTTCCATCTCTTGAATTAAGGGCTTGTCTTTCTCGCTTAATGGCACTGCCGCCAACAGACGGTAACTTTGCa                                                                                <  2:368160/150‑1 (MQ=255)                                                         aTCTTGTAAACCCTGTAATTGCGCCAGTTGTCCGTCTCGTTCTGCTGCTCGAGTCAACTCGGCGCTAAATGCCGTTTGTTCCATCTCTTGAATTAAGGGCTTGTCTTTCTCGCTTAATGGCACTGCCGCCAACAGACGGTAACTTTGCATg                                                                              >  2:168587/1‑151 (MQ=255)                                                              gTAAACCCTGTAATTGCGCCAGTTGTCCGTCTCGTTCTGCTGCTCGAGTCAACTCGGCGCTAAATGCCGTTTGTTCCATCTCTTGAATTAAGGGCTTGTCTTTCTCGCTTAATGGCACTGCCGCCAACAGACGGTAACTTTGCATGCGCa                                                                          <  1:102013/150‑1 (MQ=255)                                                                    ccTGTAATTGCGCCAGTTGTCCGTCTCGTTCTGCTGCTCGAGTCAACTCGGCGCTAAATGCCGTTTGTTCCATCTCTTGAATTAAGGGCTTGTCTTTCTCGCTTAATGGCACTGCCGCCAACAGACGGTAACTTTGCATGCGCAGCGATcc                                                                   >  2:923587/1‑151 (MQ=255)                                                                    ccTGTAATTGCGCCAGTTGTCCGTCTCGTTCTGCTGCTCGAGTCAACTCGGCGCTAAATGCCGTTTGTTCCATCTCTTGAATTAAGGGCTTGTCTTTCTCGCTTAATGGCACTGCCGCCAACAGACGGTAACTTTGCATGCGCAGCGATcc                                                                   >  1:596727/1‑151 (MQ=255)                                                                      tGTAATTGCGCCAGTTGTCCGTCTCGTTCTGCTGCTCGAGTCAACTCGGCGCTAAATGCCGTTTGTTCCATCTCTTGAATTAAGGGCTTGTCTTTCTCGCTTAATGGCACTGCCGCCAACAGACGGTAACTTTGCATGCGCAGCGATCCCg                                                                 >  2:116760/1‑151 (MQ=255)                                                                        tAATTGCGCCAGTTGTCCGTCTCGTTCTGCTGCTCGAGTCAACTCGGCGCTAAATGCCGTTTGTTCCATCTCTTGAATTAAGGGCTTGTCTTTCTCGCTTAATGGCACTGCCGCCAACAGACGGTAACTTTGCATGCGCAGCGATCCCGCt                                                               >  2:289330/1‑151 (MQ=255)                                                                         aaTTGCGCCAGTTGTCCGTCTCGTTCTGCTGCTCGAGTCAACTCGGCGCTAAATGCCGTTTGTTCCATCTCTTGAATTAAGGGCTTGTCTTTCTCGCTTAATGGCACTGCCGCCAACAGACGGTAACTTTGCATGCGCAGCGATCCCGCtt                                                              >  2:683284/1‑151 (MQ=255)                                                                         aaTTGCGCCAGTTGTCCGTCTCGTTCTGCTGCTCGAGTCAACTCGGCGCTAAATGCCGTTTGTTCCATCTCTTGAATTAAGGGCTTGTCTTTCTCGCTTAATGGCACTGCCGCCAACAGACGGTAACTTTGCATGCGCAGCGATCCCGCt                                                               <  2:560365/150‑1 (MQ=255)                                                                          aTTGCGCCAGTTGTCCGTCTCGTTCTGCTGCTCGAGTCAACTCGGCGCTAAATGCCGTTTGTTCCATCTCTTGAATTAAGGGCTTGTCTTTCTCGCTTAATGGCACTGCCGCCAACAGACGGTAACTTTGCATGCGCAGCGATCCCGCttt                                                             <  2:610025/151‑1 (MQ=255)                                                                             gcgcCAGTTGTCCGTCTCGTTCTGCTGCTCGAGTCAACTCGGCGCTAAATGCCGTTTGTTCCATCTCTTGAATTAAGGGCTTGTCTTTCTCGCTTAATGGCACTGCCGCCAACAGACGGTAACTTTGCATGCGCAGCGATCCCGCTttgtt                                                          >  2:711175/1‑151 (MQ=255)                                                                               gcCAGTTGTCCGTCTCGTTCTGCTGCTCGAGTCAACTCGGCGCTAAATGCCGTTTGTTCCATCTCTTGAATTAAGGGCTTGTCTTTCTCGCTTAATGGCACTGCCGCCAACAGACGGTAACTTTGCATGCGCAGCGATCCCGCTttgttg                                                         >  1:370951/1‑150 (MQ=255)                                                                                ccAGTTGTCCGTCTCGTTCTGCTGCTCGAGTCAACTCGGCGCTAAATGCCGTTTGTTCCATCTCTTGAATTAAGGGCTTGTCTTTCTCGCTTAATGGCACTGCCGCCAACAGACGGTAACTTTGCATGCGCAGCGATCCCGCTTTGTTGAt                                                       <  2:664902/151‑1 (MQ=255)                                                                                 cAGTTGTCCGTCTCGTTCTGCTGCTCGAGTCAACTCGGCGCTAAATGCCGTTTGTTCCATCTCTTGAATTAAGGGCTTGTCTTTCTCGCTTAATGGCACTGCCGCCAACAGACGGTAACTTTGCATGCGCAGCGATCCCGCTTTGTTGATc                                                      >  1:905614/1‑151 (MQ=255)                                                                                    ttGTCCGTCTCGTTCTGCTGCTCGAGTCAACTCGGCGCTAAATGCCGTTTGTTCCATCTCTTGAATTAAGGGCTTGTCTTTCTCGCTTAATGGCACTGCCGCCAACAGACGGTAACTTTGCATGCGCAGCGATCCCGCTTTGTTGATCGc                                                    >  1:126787/1‑150 (MQ=255)                                                                                          gTCTCGTTCTGCTGCTCGAGTCAACTCGGCGCTAAATGCCGTTTGTTCCATCTCTTGAATTAAGGGCTTGTCTTTCTCGCTTAATGGCACTGCCGCCAACAGACGGTAACTTTGCATGCGCAGCGATCCCGCTTTGTTGATCGCATGGgcg                                             >  2:326487/1‑151 (MQ=255)                                                                                          gTCTCGTTCTGCTGCTCGAGTCAACTCGGCGCTAAATGCCGTTTGTTCCATCTCTTGAATTAAGGGCTTGTCTTTCTCGCTTAATGGCACTGCCGCCAACAGACGGTAACTTTGCATGCGCAGCGATCCCGCTTTGTTGATCGCATGGgc                                              >  2:93970/1‑150 (MQ=255)                                                                                           tctcGTTCTGCTGCTCGAGTCAACTCGGCGCTAAATGCCGTTTGTTCCATCTCTTGAATTAAGGGCTTGTCTTTCTCGCTTAATGGCACTGCCGCCAACAGACGGTAACTTTGCATGCGCAGCGATCCCGCTTTGTTGATCGCATGGgcgc                                            >  1:917763/1‑151 (MQ=255)                                                                                              cGTTCTGCTGCTCGAGTCAACTCGGCGCTAAATGCCGTTTGTTCCATCTCTTGAATTAAGGGCTTGTCTTTCTCGCTTAATGGCACTGCCGCCAACAGACGGTAACTTTGCATGCGCAGCGATCCCGCTTTGTTGATCGCATGGGCGCt                                           <  2:112578/149‑1 (MQ=255)                                                                                              cGTTCTGCTGCTCGAGTCAACTCGGCGCTAAATGCCGTTTGTTCCATCTCTTGAATTAAGGGCTTGTCTTTCTCGCTTAATGGCACTGCCGCCAACAGACGGTAACTTTGCATGCGCAGCGATCCCGCTTTGTTGATCGCATGGGCGCTGc                                         >  1:698481/1‑151 (MQ=255)                                                                                              cGTTCTGCTGCTCGAGTCAACTCGGCGCTAAATGCCGTTTGTTCCATCTCTTGAATTAAGGGCTTGTCTTTCTCGCTTAATGGCACTGCCGCCAACAGACGGTAACTTTGCATGCGCAGCGATCCCGCTTTGTTGATCGCATGGGCGCTGc                                         >  1:745124/1‑151 (MQ=255)                                                                                                    gctgctCGAGTCAACTCGGCGCTAAATGCCGTTTGTTCCATCTCTTGAATTAAGGGCTTGTCTTTCTCGCTTAATGGCACTGCCGCCAACAGACGGTAACTTTGCATGCGCAGCGATCCCGCTTTGTTGATCGCATGGGCGCTGCCCt                                      <  2:711102/148‑1 (MQ=255)                                                                                                    gctgctCGAGTCAACTCGGCGCTAAATGCCGTTTGTTCCATCTCTTGAATTAAGGGCTTGTCTTTCTCGCTTAATGGCACTGCCGCCAACAGACGGTAACTTTGCATGCGCAGCGATCCCGCTTTGTTGATCGCATGGGCGCTGCCCTGaa                                   <  1:919525/151‑1 (MQ=255)                                                                                                          cGAGTCAACTCGGCGCTAAATGCCGTTTGTTCCATCTCTTGAATTAAGGGCTTGTCTTTCTCGCTTAATGGCACTGCCGCCAACAGACGGTAACTTTGCATGCGCAGCGATCCCGCTTTGTTGATCGCATGGGCGCTGCCCTGAACGCCt                              <  2:544358/150‑1 (MQ=255)                                                                                                           gAGTCAACTCGGCGCTAAATGCCGTTTGTTCCATCTCTTGAATTAAGGGCTTGTCTTTCTCGCTTAATGGCACTGCCGCCAACAGACGGTAACTTTGCATGCGCAGCGATCCCGCTTTGTTGATCGCATGGGCGCTGCCCTGAACGCCTTg                            <  2:719579/151‑1 (MQ=255)                                                                                                            aGTCAACTCGGCGCTAAATGCCGTTTGTTCCATCTCTTGAATTAAGGGCTTGTCTTTCTCGCTTAATGGCACTGCCGCCAACAGACGGTAACTTTGCATGCGCAGCGATCCCGCTTTGTTGATCGCATGGGCGCTGCCCTGAACGCCTTGc                           >  2:22147/1‑151 (MQ=255)                                                                                                              tCAACTCGGCGCTAAATGCCGTTTGTTCCATCTCTTGAATTAAGGGCTTGTCTTTCTCGCTTAATGGCACTGCCGCCAACAGACGGTAACTTTGCATGCGCAGCGATCCCGCTTTGTTGATCGCATGGGCGCTGCCCTGAACGCCTTGCAc                         >  2:606137/1‑151 (MQ=255)                                                                                                                           aaaTGCCGTTTGTTCCATCTCTTGAATTAAGGGCTTGTCTTTCTCGCTTAATGGCACTGCCGCCAACAGACGGTAACTTTGCATGCGCAGCGATCCCGCTTTGTTGATCGCATGGGCGCTGCCCTGAACGCCTTGCAccagccagccag              <  2:28097/149‑1 (MQ=255)                                                                                                                           aaaTGCCGTTTGTTCCATCTCTTGAATTAAGGGCTTGTCTTTCTCGCTTAATGGCACTGCCGCCAACAGACGGTAACTTTGCATGCGCAGCGATCCCGCTTTGTTGATCGCATGGGCGCTGCCCTGAACGCCTTGCACCAGCCAGCCAGa             >  2:696595/1‑150 (MQ=255)                                                                                                                           aaaTGCCGTTTGTTCCATCTCTTGAATTAAGGGCTTGTCTTTCTCGCTTAATGGCACTGCCGCCAACAGACGGTAACTTTGCATGCGCAGCGATCCCGCTTTGTTGATCGCATGGGCGCTGCCCTGAACGCCTTGCACCAGCCAGCCAGa             <  2:656900/150‑1 (MQ=255)                                                                                                                                ccGTTTGTTCCATCTCTTGAATTAAGGGCTTGTCTTTCTCGCTTAATGGCACTGCCGCCAACAGACGGTAACTTTGCATGCGCAGCGATCCCGCTTTGTTGATCGCATGGGCGCTGCCCTGAACGCCTTGCACCAGCCAGCCAGAAACCGc       >  2:631258/1‑151 (MQ=255)                                                                                                                                    ttGTTCCATCTCTTGAATTAAGGGCTTGTCTTTCTCGCTTAATGGCACTGCCGCCAACAGACGGTAACTTTGCATGCGCAGCGATCCCGCTTTGTTGATCGCATGGGCGCTGCCCTGAACGCCTTGCACCAGCCAGCCAGAAACCGCCATc   >  2:357634/1‑151 (MQ=255)                                                                                                                                       ttCCATCTCTTGAATTAAGGCCTTGTCTTTCTCGCTTAATGGCACTGCCGCCAACAGCCGGTAACTTTGTATGCGCAGCGATCCCGCTTTGTTGATCGCATGGGCGCTGCCCTGAACGCGTTGCACCAGCCAGCCAGAAACCGCCATcc  >  1:777716/1‑149 (MQ=255)                                                                                                                                                  |                                                                                                                                          ACCGTTTCTCGGTTTTGTGCACGCATCAGCGCAGGGATCAGTTCATTACGCCAGTAATCTTGTAAACCCTGTAATTGCGCCAGTTGTCCGTCTCGTTCTGCTGCTCGAGTCAACTCGGCGCTAAATGCCGTTTGTTCCATCTCTTTAATTAAGGGCTTGTCTTTCTCGCTTAATGGCACTGCCGCCAACAGACGGTAACTTTGCATGCGCAGCGATCCCGCTTTGTTGATCGCATGGGCGCTGCCCTGAACGCCTTGCACCAGCCAGCCAGAAACCGCCATCC  >  NC_000913/1277251‑1277533

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑