Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,323,663	T→G	L148L (CTT→CTG)	yciV →	PHP domain protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,323,663	0	T	G	100.0%	165.5 / NA	45	L148L (CTT→CTG)	yciV	PHP domain protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (21/24);  total (21/24)

CCTCGCGCAGCAGACAGAACGGCGCAATCAGCGGGCGCAGCTGATTGCCGAAAGACTTGAAAAAGCGCAAATACCTGGGGCGCTGGAAGGCGCACAACGACTGGCGCAGGGCGGCGCAGTGACGCGCGGTCATTTTGCGCGTTTCCTTGTTGAGTGCGGCAAAGCCAGTTCAATGGCGGATGTCTTTAAAAAGTATCTGGCGCGCGGGAAAACCGGATACGTTCCGCCACAGTGGTGTACAATAGAACAAGCTATTGATGTCATTCATCATTCTGGCGGTAAGGCGGTGCTA  >  NC_000913/1323516‑1323807                                                                                                                                                    |                                                                                                                                                 ccTCGCGCAGCAGACAGAACGGCGCAATCAGCGGGCGCAGCTGATTGCCGAAAGACTTGAAAAAGCGCAAATACCTGGGGCGCTGGAAGGCGCACAACGACTGGCGCAGGGCGGCGCAGTGACGCGCGGTCATTTTGCGCGTTTCCtggtt                                                                                                                                               >  2:531967/1‑151 (MQ=255) ccTCGCGCAGCAGACAGAACGGCGCAATCAGCGGGCGCAGCTGATTGCCGAAAGACTTGAAAAAGCGCAAATACCTGGGGCGCTGGAAGGCGCACAACGACTGGCGCAGGGCGGCGCAGTGACGCGCGGTCATTTTGCGCGTTTCCtggt                                                                                                                                                <  2:351779/150‑1 (MQ=255)   tCGCGCAGCAGACAGAACGGCGCAATCAGCGGGCGCAGCTGATTGCCGAAAGACTTGAAAAAGCGCAAATACCTGGGGCGCTGGAAGGCGCACAACGACTGGCGCAGGGCGGCGCAGTGACGCGCGGTCATTTTGCGCGTTTCCtggttg                                                                                                                                              >  2:614349/1‑150 (MQ=255)    cgcgCAGCAGACAGAACGGCGCAATCAGCGGGCGCAGCTGATTGCCGAAAGACTTGAAAAAGCGCAAATACCTGGGGCGCTGGAAGGCGCACAACGACTGGCGCAGGGCGGCGCAGTGACGCGCGGTCATTTTGCGCGTTTCCTGGTTGAg                                                                                                                                            <  1:222551/151‑1 (MQ=255)      cgcAGCAGACAGAACGGCGCAATCAGCGGGCGCAGCTGATTGCCGAAAGACTTGAAAAAGCGCAAATACCTGGGGCGCTGGAAGGCGCACAACGACTGGCGCAGGGCGGCGCAGTGACGCGCGGTCATTTTGCGCGTTTCCTGGTTGAGTg                                                                                                                                          >  2:298590/1‑151 (MQ=255)               cagaACGGCGCAATCAGCGGGCGCAGCTGATTGCCGAAAGACTTGAAAAAGCGCAAATACCTGGGGCGCTGGAAGGCGCACAACGACTGGCGCAGGGCGGCGCAGTGACGCGCGGTCATTTTGCGCGTTTCCTGGTTGAGTGCGGCaa                                                                                                                                    <  2:304701/148‑1 (MQ=255)                   aCGGCGCAATCAGCGGGCGCAGCTGATTGCCGAACGACTTGAAAAAGCGCAAATACCTGGGGCGCTGGAAGGCGCACAACGACTGGCGCAGGGCGGCGCAGTGACGCGCGGTCATTTTGCGCGTTTCCTGGTTGAGTGCGGCAAAGCCAg                                                                                                                              >  1:703812/1‑150 (MQ=255)                    cGGCGCAATCAGCGGGCGCAGCTGATTGCCGAAAGACTTGAAAAAGCGCAAATACCTGGGGCGCTGGAAGGCGCACAACGACTGGCGCAGGGCGGCGCAGTGACGCGCGGTCATTTTGCGCGTTTCCTGGTTGAGTGCGGCAAAGCCAGtt                                                                                                                            <  1:774039/151‑1 (MQ=255)                       cgcAATCAGCGGGCGCAGCTGATTGCCGAAAGACTTGAAAAAGCGCAAATACCTGGGGCGCTGGAAGGCGCACAACGACTGGCGCAGGGCGGCGCAGTGACGCGCGGTCATTTTGCGCGTTTCCTGGTTGAGTGCGGCAAAGCCAGTTCa                                                                                                                          >  1:279940/1‑150 (MQ=255)                        gcAATCAGCGGGCGCAGCTGATTGCCGAAAGACTTGAAAAAGCGCAAATACCTGGGGCGCTGGAAGGCGCACAACGACTGGCGCAGGGCGGCGCAGTGACGCGCGGTCATTTTGCGCGTTTCCTGGTTGAGTGCGGCAAAGCCAGTTCAAt                                                                                                                        <  1:637869/151‑1 (MQ=255)                          aaTCAGCGGGCGCAGCTGATTGCCGAAAGACTTGAAAAAGCGCAAATACCTGGGGCGCTGGAAGGCGCACAACGACTGGCGCAGGGCGGCGCAGTGACGCGCGGTCATTTTGCGCGTTTCCTGGTTGAGTGCGGCAAAGCCAGTTCAATgg                                                                                                                      <  2:725682/151‑1 (MQ=255)                          aaTCAGCGGGCGCAGCTGATTGCCGAAAGACTTGAAAAAGCGCAAATACCTGGGGCGCTGGAAGGCGCACAACGACTGGCGCAGGGCGGCGCAGTGACGCGCGGTCATTTTGCGCGTTTCCTGGTTGAGTGCGGCAAAGCCAGTTCAATgg                                                                                                                      >  2:246319/1‑151 (MQ=255)                                cGGGCGCAGCTGATTGCCGAAAGACTTGAAAAAGCGCAAATACCTGGGGCGCTGGAAGGCGCACAACGACTGGCGCAGGGCGGCGCAGTGACGCGCGGTCATTTTGCGCGTTTCCTGGTTGAGTGCGGCAAAGCCAGTTCAATGGCGGATg                                                                                                                <  1:15763/151‑1 (MQ=255)                                   gcgcAGCTGATTGCCGAAAGACTTGAAAAAGCGCAAATACCTGGGGCGCTGGAAGGCGCACAACGACTGGCGCAGGGCGGCGCAGTGACGCGCGGTCATTTTGCGCGTTTCCTGGTTGAGTGCGGCAAAGCCAGTTCAATGGCGGATGTCt                                                                                                             <  2:313637/151‑1 (MQ=255)                                     gcAGCTGATTGCCGAAAGACTTGAAAAAGCGCAAATACCTGGGGCGCTGGAAGGCGCACAACGACTGGCGCAGGGCGGCGCAGTGACGCGCGGTCATTTTGCGCGTTTCCTGGTTGAGTGCGGCAAAGCCAGTTCAATGGCGGATGTCttt                                                                                                           >  2:538510/1‑151 (MQ=255)                                     gcAGCTGATTGCCGAAAGACTTGAAAAAGCGCAAATACCTGGGGCGCTGGAAGGCGCACAACGACTGGCGCAGGGCGGCGCAGTGACGCGCGGTCATTTTGCGCGTTTCCTGGTTGAGTGCGGCAAAGCCAGTTCAATGGCGGATGTCttt                                                                                                           <  1:423092/151‑1 (MQ=255)                                               gCCGAAAGACTTGAAAAAGCGCAAATACCTGGGGCGCTGGAAGGCGCACAACGACTGGCGCAGGGCGGCGCAGTGACGCGCGGTCATTTTGCGCGTTTCCTGGTTGAGTGCGGCAAAGCCAGTTCAATGGCGGATGTCTTTAAAAAGTATc                                                                                                 <  2:627684/151‑1 (MQ=255)                                                    aaGACTTGAAAAAGCGCAAATACCTGGGGCGCTGGAAGGCGCACAACGACTGGCGCAGGGCGGCGCAGTGACGCGCGGTCATTTTGCGCGTTTCCTGGTTGAGTGCGGCAAAGCCAGTTCAATGGCGGATGTCTTTAAAAAGTATCTGgcg                                                                                            >  1:217156/1‑151 (MQ=255)                                                     aGACTTGAAAAAGCGCAAATACCTGGGGCGCTGGAAGGCGCACAACGACTGGCGCAGGGCGGCGCAGTGACGCGCGGTCATTTTGCGCGTTTCCTGGTTGAGTGCGGCAAAGCCAGTTCAATGGCGGATGTCTTTAAAAAGTATCTGgcgc                                                                                           >  2:469305/1‑151 (MQ=255)                                                          tGAAAAAGCGCAAATACCTGGGGCGCTGGAAGGCGCACAACGACTGGCGCAGGGCGGCGCAGTGACGCGCGGTCATTTTGCGCGTTTCCTGGTTGAGTGCGGCAAAGCCAGTTCAATGGCGGATGTCTTTAAAAAGTATCTGGCGCGCggg                                                                                      >  1:104637/1‑151 (MQ=255)                                                                      aaTACCTGGGGCGCTGGAAGGCGCACAACGACTGGCGCAGGGCGGCGCAGTGACGCGCGGTCATTTTGCGCGTTTCCTGGTTGAGTGCGGCAAAGCCAGTTCAATGGCGGATGTCTTTAAAAAGTATCTGGCGCGCGGGAAAACCGGATAc                                                                          >  1:193967/1‑151 (MQ=255)                                                                      aaTACCTGGGGCGCTGGAAGGCGCACAACGACTGGCGCAGGGCGGCGCAGTGACGCGCGGTCATTTTGCGCGTTTCCTGGTTGAGTGCGGCAAAGCCAGTTCAATGGCGGATGTCTTTAAAAAGTATCTGGCGCGCGGGAAAACCGGATAc                                                                          >  1:902722/1‑151 (MQ=255)                                                                            tGGGGCGCTGGAAGGCGCACAACGACTGGCGCAGGGCGGCGCAGTGACGCGCGGTCATTTTGCGCGTTTCCTGGTTGAGTGCGGCAAAGCCAGTTCAATGGCGGATGTCTTTAAAAAGTATCTGGCGCGCGGGAAAACCGGATACGTTCCg                                                                    >  2:335570/1‑151 (MQ=255)                                                                                        aGGCGCACAACGACTGGCGCAGGGCGGCGCAGTGACGCGCGGTCATTTTGCGCGTTTCCTGGTTGAGTGCGGCAAAGCCAGTTCAATGGCGGATGTCTTTAAAAAGTATCTGGCGCGCGGGAAAACCGGATACGTTCCGCCACAGTGgtgt                                                        <  1:12213/151‑1 (MQ=255)                                                                                             cacaACGACTGGCGCAGGGCGGCGCAGTGACGCGCGGTCATTTTGCGCGTTTCCTGGTTGAGTGCGGCAAAGCCAGTTCAATGGCGGATGTCTTTAAAAAGTATCTGGCGCGCGGGAAAACCGGATACGTTCCGCCACAGTGGTGTACAAt                                                   >  2:212148/1‑151 (MQ=255)                                                                                             cacaACGACTGGCGCAGGGCGGCGCAGTGACGCGCGGTCATTTTGCGCGTTTCCTGGTTGAGTGCGGCAAAGCCAGTTCAATGGCGGATGTCTTTAAAAAGTATCTGGCGCGCGGGAAAACCGGATACGTTCCGCCACAGTGGTGTACAAt                                                   <  1:298581/151‑1 (MQ=255)                                                                                               caACGACTGGCGCAGGGCGGCGCAGTGACGCGCGGTCATTTTGCGCGTTTCCTGGTTGAGTGCGGCAAAGCCAGTTCAATGGCGGATGTCTTTAAAAAGTATCTGGCGCGCGGGAAAACCGGATACGTTCCGCCACAGTGGTGTACAAt                                                   >  1:96553/1‑149 (MQ=255)                                                                                                 aCGACTGGCGCAGGGCGGCGCAGTGACGCGCGGTCATTTTGCGCGTTTCCTGGTTGAGTGCGGCAAAGCCAGTTCAATGGCGGATGTCTTTAAAAAGTATCTGGCGCGCGGGAAAACCGGATACGTTCCGCCACAGTGGTGTACAATAGaa                                               <  1:283485/151‑1 (MQ=255)                                                                                                   gACTGGCGCAGGGCGGCGCAGTGCCGCGCGGTCATTTTGCGCGTTTCCTGGTTGAGTGCGGCAAAGCCAGTTCAATGGCGGATGTCTTTAAAAAGTATCTGGCGCGCGGGAAAACCGGATACGTTCCGCCACAGCGGTGAACAATAGaa                                               >  1:869557/1‑149 (MQ=255)                                                                                                     cTGGCGCAGGGCGGCGCAGTGACGCGCGGTCATTTTGCGCGTTTCCTGGTTGAGTGCGGCAAAGCCAGTTCAATGGCGGATGTCTTTAAAAAGTATCTGGCGCGCGGGAAAACCGGATACGTTCCGCCACAGTGGTGTACAATAGAACa                                             >  1:496112/1‑149 (MQ=255)                                                                                                      tGGCGCAGGGCGGCGCAGTGACGCGCGGTCATTTTGCGCGTTTCCTGGTTGAGTGCGGCAAAGCCAGTTCAATGGCGGATGTCTTTAAAAAGTATCTGGCGCGCGGGAAAACCGGATACGTTCCGCCACAGTGGTGTACAATAGAACaa                                            >  1:510114/1‑149 (MQ=255)                                                                                                       ggCGCAGGGCGGCGCAGTGACGCGCGGTCATTTTGCGCGTTTCCTGGTTGAGTGCGGCAAAGCCAGTTCAATGGCGGATGTCTTTAAAAAGTATCTGGCGCGCGGGAAAACCGGATACGTTCCGCCACAGTGGTGTACAATAGAACAAg                                           <  1:115398/149‑1 (MQ=255)                                                                                                       ggCGCAGGGCGGCGCAGTGACGCGCGGTCATTTTGCGCGTTTCCTGGTTGAGTGCGGCAAAGCCAGTTCAATGGCGGATGTCTTTAAAAAGTATCTGGCGCGCGGGAAAACCGGATACGTTCCGCCACAGTGGTGTACAATAGAACAAGCt                                         >  2:633986/1‑151 (MQ=255)                                                                                                       ggCGCAGGGCGGCGCAGTGACGCGCGGTCATTTTGCGCGTTTCCTGGTTGAGTGCGGCAAAGCCAGTTCAATGGCGGATGTCTTTAAAAAGTATCTGGCGCGCGGGAAAACCGGATACGTTCCGCCACAGTGGTGTACAATAGAACAAGCt                                         <  1:41284/151‑1 (MQ=255)                                                                                                             gggCGGCGCAGTGACGCGCGGTCATTTTGCGCGTTTCCTGGTTGAGTGCGGCAAAGCCAGTTCAATGGCGGATGTCTTTAAAAAGTATCTGGCGCGCGGGAAAACCGGATACGTTCCGCCACAGTGGTGTACAATAGAACAAGCTATTGAt                                   <  1:545361/151‑1 (MQ=255)                                                                                                               gcggcgCAGTGACGCGCGGTCATTTTGCGCGTTTCCTGGTTGAGTGCGGCAAAGCCAGTTCAATGGCGGATGTCTTTAAAAAGTATCTGGCGCGCGGGAAAACCGGATACGTTCCGCCACAGTGGTGTACAATAGAACAAGCTATTGATGt                                 >  2:692145/1‑151 (MQ=255)                                                                                                                    gcAGTGACGCGCGGTCATTTTGCGCGTTTCCTGGTTGAGTGCGGCAAAGCCAGTTCAATGGCGGATGTCTTTAAAAAGTATCTGGCGCGCGGGAAAACCGGATACGTTCCGCCACAGTGGTGTACAATAGAACAAGCTATTGATGtcatt                             <  1:388365/150‑1 (MQ=255)                                                                                                                      aGTGACGCGCGGTCATTTTGCGCGTTTCCTGGTTGAGTGCGGCAAAGCCAGTTCAATGGCGGATGTCTTTAAAAAGTATCTGGCGCGCGGGAAAACCGGATACGTTCCGCCACAGTGGTGTACAATAGAACAAGCTATTGATGtcattcat                          >  2:464805/1‑151 (MQ=255)                                                                                                                         gACGCGCGGTCATTTTGCGCGTTTCCTGGTTGAGTGCGGCAAAGCCAGTTCAATGGCGGATGTCTTTAAAAAGTATCTGGCGCGCGGGAAAACCGGATACGTTCCGCCACAGTGGTGTACAATAGAACAAGCTATTGATGTCATTcatcat                       <  1:593002/151‑1 (MQ=255)                                                                                                                         gACGCGCGGTCATTTTGCGCGTTTCCTGGTTGAGTGCGGCAAAGCCAGTTCAATGGCGGATGTCTTTAAAAAGTATCTGGCGCGCGGGAAAACCGGATACGTTCCGCCACAGTGGTGTACAATAGAACAAGCTATTGATGTCATTcatcat                       >  2:646377/1‑151 (MQ=255)                                                                                                                          aCGCGCGGTCATTTTGCGCGTTTCCTGGTTGAGTGCGGCAAAGCCAGTTCAATGGCGGATGTCTTTAAAAAGTATCTGGCGCGCGGGAAAACCGGATACGTTCCGCCACAGTGGTGTACAATAGAACAAGCTATTGATGTCATTCATCAtt                      <  2:37694/151‑1 (MQ=255)                                                                                                                           cgcgcgGTCATTTTGCGCGTTTCCTGGTTGAGTGCGGCAAAGCCAGTTCAATGGCGGATGTCTTTAAAAAGTATCTGGCGCGCGGGAAAACCGGATACGTTCCGCCACAGTGGTGTACAATAGAACAAGCTATTGATGTCATTCATCATTc                     <  1:260786/151‑1 (MQ=255)                                                                                                                            gcgcgGTCATTTTGCGCGTTTCCTGGTTGAGTGCGGCAAAGCCAGTTCAATGGCGGATGTCTTTAAAAAGTATCTGGCGCGCGGGAAAACCGGATACGTTCCGCCACAGTGGTGTACAATAGAACAAGCTATTGATGTCATTCATCATTCt                    <  1:255201/151‑1 (MQ=255)                                                                                                                              gcgGTCATTTTGCGCGTTTCCTGGTTGAGTGCGGCAAAGCCAGTTCAATGGCGGATGTCTTTAAAAAGTATCTGGCGCGCGGGAAAACCGGATACGTTCCGCCACAGTGGGGTACAATAGAACAAGCTATTGATGTCATTCATCATTCTgg                  >  1:172919/1‑151 (MQ=255)                                                                                                                               cgGTCATTTTGCGCGGTTCCTGGTTGAGTGCGGCAAAGCCAGTTCAAGGGCGGATGTCTTTAAAAAGTATCGGGCGCGCGGGAAAACCGGATACGTTCCGCCACAGGGGTGTACAATAGAACAAGCTATTGATGTCATTCATCATTCTg                   <  1:471494/149‑1 (MQ=255)                                                                                                                                       ttGCGCGTTTCCTGGTTGAGTGCGGCAAAGCCAGTTCAATGGCGGATGTCTTTAAAAAGTATCTGGCGCGCGGGAAAACCGGATACGTTCCGCCACAGTGGTGTACAATAGAACAAGCTATTGATGTCATTCATCATTCTGGCGGTAAgg          <  1:604417/150‑1 (MQ=255)                                                                                                                                        tGCGCGTTTCCTGGTTGAGTGCGGCAAAGCCAGTTCAATGGCGGATGTCTTTAAAAAGTATCTGGCGCGCGGGAAAACCGGATACGTTCCGCCACAGTGGTGTACAATAGAACAAGCTATTGATGTCATTCATCATTCTGGCGGTAAGGc         <  1:855033/150‑1 (MQ=255)                                                                                                                                               ttCCTGGTTGAGTGCGGAAAAGCCAGTTCAATGGCGGATGTCTTTAAAAAGTATCTGGCGCGCGGGAAAACCGGATACGTTCCGCCACAGTGGTGTACAATAGAACAAGCTATTGATGTCATTCATCATTCTGGCGGTAAGGCGGTGCTa  <  1:142434/150‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                 CCTCGCGCAGCAGACAGAACGGCGCAATCAGCGGGCGCAGCTGATTGCCGAAAGACTTGAAAAAGCGCAAATACCTGGGGCGCTGGAAGGCGCACAACGACTGGCGCAGGGCGGCGCAGTGACGCGCGGTCATTTTGCGCGTTTCCTTGTTGAGTGCGGCAAAGCCAGTTCAATGGCGGATGTCTTTAAAAAGTATCTGGCGCGCGGGAAAACCGGATACGTTCCGCCACAGTGGTGTACAATAGAACAAGCTATTGATGTCATTCATCATTCTGGCGGTAAGGCGGTGCTA  >  NC_000913/1323516‑1323807

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑