Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,334,673 T→G G273G (GGT→GGG)  cysB → N‑acetylserine‑responsive cysteine regulon transcriptional activator; autorepressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,334,6730TG97.9% 170.2 / ‑3.6 48G273G (GGT→GGG) cysBN‑acetylserine‑responsive cysteine regulon transcriptional activator; autorepressor
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base G (27/20);  total (28/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.15e-01

CAACGGATGCTGACGTCATTAAAACTTACGTCCGGTTAGGGCTGGGGGTAGGGGTCATTGCCAGCATGGCGGTGGATCCGGTCGCCGATCCCGACCTTGTGCGTGTTGATGCTCACGATATCTTCAGCCACAGTACAACCAAAATTGGTTTTCGCCGTAGTACTTTCTTGCGCAGTTATATGTATGATTTCATTCAGCGTTTTGCACCGCATTTAACGCGTGATGTCGTTGATGCGGCTGTCGCATTGCGCTCTAATGAAGAAATTGAGGTCATGTTTAAAGATATAAAACTG  >  NC_000913/1334525‑1334817
                                                                                                                                                    |                                                                                                                                                
cAACGGATGCTGACGTCATTAAAACTTACGTCCGGTTAGGGCTGGGGGTAGGGGTCATTGCCAGCATGGCGGTGGATCCGGTCGCCGATCCCGACCTTGTGCGTGTTGATGCTCACGATATCTTCAGCCACAGTACAACCAAAATTGGgtt                                                                                                                                                <  2:599364/151‑1 (MQ=255)
  aCGGATGCTGACGTCATTAAAACTTACGTCCGGTTAGGGCTGGGGGTAGGGGTCATTGCCAGCATGGCGGTGGATCCGGTCGCCGATCCCGACCTTGTGCGTGTTGATGCTCACGATATCTTCAGCCACAGTACAACCAAAATTGGGTTTc                                                                                                                                              >  1:153483/1‑151 (MQ=255)
    ggATGCTGACGTCATTAAAACTTACGTCCGGTTAGGGCTGGGGGTAGGGGTCATTGCCAGCATGGCGGTGGATCCGGTCGCCGATCCCGACCTTGTGCGTGTTGATGCTCACGATATCTTCAGCCACAGTACAACCAAAATTGGGTTTCGc                                                                                                                                            >  1:497912/1‑151 (MQ=255)
        gCTGACGTCATTAAAACTTACGTCCGGTTAGGGCTGGGGGTAGGGGTCATTGCCAGCATGGCGGTGGATCCGGTCGCCGATCCCGACCTTGTGCGTGTTGATGCTCACGATATCTTCAGCCACAGTACAACCAAAATTGGGTTTCGCCgta                                                                                                                                        <  2:428131/151‑1 (MQ=255)
         cTGACGTCATTAAAACTTACGTCCGGTTAGGGCTGGGGGTAGGGGTCATTGCCAGCATGGCGGTGGATCCGGTCGCCGATCCCGACCTTGTGCGTGTTGATGCTCACGATATCTTCAGCCACAGTACAACCAAAATTGGGTTTCGCCgt                                                                                                                                         >  1:468389/1‑149 (MQ=255)
             cGTCATTAAAACTTACGTCCGGTTAGGGCTGGGGGTAGGGGTCATTGCCAGCATGGCGGTGGATCCGGTCGCCGATCCCGACCTTGTGCGTGTTGATGCTCACGATATCTTCAGCCACAGTACAACCAAAATTGGGTTTCGCCGTAGTACt                                                                                                                                   >  2:553326/1‑151 (MQ=255)
                     aaaCTTACGTCCGGTTAGGGCTGGTGGTAGGGGTCATTGCCAGCATGGCCGTGGATCCGGTCGGCGATCCTGACCTTGTGCGTGTTGATGCTCACGATATCTTCAGCCACAGTACAACCAAAAGTGGGTTTCGCCGTAGGACTTTCTTgcg                                                                                                                           <  1:629870/151‑1 (MQ=255)
                      aaCTTACGTCCGGTTAGGGCTGGGGGTAGGGGTCATTGCCAGCATGGCGGTGGATCCGGTCGCCGATCCCGACCTTGTGCGTGTTGATGCTCACGATATCTTCAGCCACAGTACAACCAAAATTGGGTTTCGCCGTAGTACTTTCTTgcgc                                                                                                                          >  2:149049/1‑151 (MQ=255)
                            cGTCCGGTTAGGGCTGGGGGTAGGGGTCATTGCCAGCATGGCGGTGGATCCGGTCGCCGATCCCGACCTTGTGCGTGTTGATGCTCACGATATCTTCAGCCACAGTACAACCAAAATTGGGTTTCGCCGTAGTACTTTCTTGCGCAGTtat                                                                                                                    >  1:202168/1‑151 (MQ=255)
                             gTCCGGTTAGGGCTGGGGGTAGGGGTCATTGCCAGCATGGCGGTGGATCCGGTCGCCGATCCCGACCTTGTGCGTGTTGATGCTCACGATATCTTCAGCCACAGTACAACCAAAATTGGGTTTCGCCGTAGTACTTTCTTGCGCAGTtat                                                                                                                    >  2:646297/1‑150 (MQ=255)
                                            ggggTAGGGGTCATTGCCAGCATGGCGGTGGATCCGGTCGCCGATCCCGACCTTGTGCGTGTTGATGCTCACGATATCTTCAGCCACAGTACAACCAAAATTGGGTTTCGCCGTAGTACTTTCTTGCGCAGTTATATGTATGATttcattc                                                                                                    <  2:651237/151‑1 (MQ=255)
                                              ggTAGGGGTCATTGCCAGCATGGCGGTGGATCCGGTCGCCGATCCCGACCTTGTGCGTGTTGATGCTCACGATATCTTCAGCCACAGTACAACCAAAATTGGGTTTCGCCGTAGTACTTTCTTGCGCAGTTATATGTATGATttcattc                                                                                                    >  1:695581/1‑149 (MQ=255)
                                               gTAGGGGTCATTGCCAGCATGGCGGTGGATCCGGTCGCCGATCCCGACCTTGTGCGTGTTGATGCTCACGATATCTTCAGCCACAGTACAACCAAAATTGGGTTTCGCCGTAGTACTTTCTTGCGCAGTTATATGTATGATTTCATTCAGc                                                                                                 <  1:546315/151‑1 (MQ=255)
                                               gTAGGGGTCATTGCCAGCATGGCGGTGGATCCGGTCGCCGATCCCGACCTTGTGCGTGTTGATGCTCACGATATCTTCAGCCACAGTACAACCAAAATTGGGTTTCGCCGTAGTACTTTCTTGCGCAGTTATATGTATGATTTCATTCAGc                                                                                                 <  1:848509/151‑1 (MQ=255)
                                                        aTTGCCAGCATGGCGGTGGATCCGGTCGCCGATCCCGACCTTGTGCGTGTTGATGCTCACGATATCTTCAGCCACAGTACAACCAAAATTGGGTTTCGCCGTAGTACTTTCTTGCGCAGTTATATGTATGATTTCATTCAGCGTTTTGCAc                                                                                        >  2:160759/1‑151 (MQ=255)
                                                          tGCCAGCATGGCGGTGGATCCGGTCGCCGATCCCGACCTTGTGCGTGTTGATGCTCACGATATCTTCAGCCACAGTACAACCAAAATTGGGTTTCGCCGTAGTACTTTCTTGCGCAGTTATATGTATGATTTCATTCAGCGTTTTGCAcc                                                                                       <  1:57230/150‑1 (MQ=255)
                                                            ccAGCATGGCGGTGGATCCGGTCGCCGATCCCGACCTTGTGCGTGTTTATGCTCACGATATCTTCAGCCACAGTACAACCAAAATTGGGTTTCGCCGTAGTACTTTCTTGCGCAGTTATATGTATGATTTCATTCAGCGTTTTGCACCGc                                                                                     >  2:565431/1‑150 (MQ=255)
                                                                 aTGGCGGTGGATCCGGTCGCCGATCCCGACCTTGTGCGTGTTGATGCTCACGATATCTTCAGCCACAGTACAACCAAAATTGGGTTTCGCCGTAGTACTTTCTTGCGCAGTTATATGTATGATTTCATTCAGCGTTTTGCACCGCATTTaa                                                                               <  2:724767/151‑1 (MQ=255)
                                                                    gCGGTGGATCCGGTCGCCGATCCCGACCTTGTGCGTGTTGATGCTCACGATATCTTCAGCCACAGTACAACCAAAATTGGGTTTCGCCGTAGTACTTTCTTGCGCAGTTATATGTATGATTTCATTCAGCGTTTTGCACCGCATTTAAcgc                                                                            <  2:377532/151‑1 (MQ=255)
                                                                          gATCCGGTCGCCGATCCCGACCTTGTGCGTGTTGATGCTCACGATATCTTCAGCCACAGTACAACCAAAATTGGGTTTCGCCGTAGTACTTTCTTGCGCAGTTATATGTATGATTTCATTCAGCGTTTTGCACCGCATTTAACGCGTGATg                                                                      <  1:246232/151‑1 (MQ=255)
                                                                             ccGGTCGCCGATCCCGACCTTGTGCGTGTTGATGCTCACGATATCTTCAGCCACAGTACAACCAAAATTGGGTTTCGCCGTAGTACTTTCTTGCGCAGTTATATGTATGATTTCATTCAGCGTTTTGCACCGCATTTAACGCGTGATGTCg                                                                   <  1:553308/151‑1 (MQ=255)
                                                                                  cGCCGATCCCGACCTTGTGCGTGTTGATGCTCACGATATCTTCAGCCACAGTACAACCAAAATTGGGTTTCGCCGTAGTACTTTCTTGCGCAGTTATATGTATGATTTCATTCAGCGTTTTGCACCGCATTTAACGCGTGATGTCGTTGAt                                                              >  1:322292/1‑151 (MQ=255)
                                                                                  cGCCGATCCCGACCTTGTGCGTGTTGATGCTCACGATATCTTCAGCCACAGTACAACCAAAATTGGGTTTCGCCGTAGTACTTTCTTGCGCAGTTATATGTATGATTTCATTCAGCGTTTTGCACCGCATTTAACGCGTGATGTCGTTGAt                                                              <  2:863628/151‑1 (MQ=255)
                                                                                      gATCCCGACCTTGTGCGTGTTGATGCTCACGATATCTTCAGCCACAGTACAACCAAAATTGGGTTTCGCCGTAGTACTTTCTTGCGCAGTTATATGTATGATTTCATTCAGCGTTTTGCACCGCATTTAACGCGTGATGTCGTTGATGCgg                                                          >  1:235586/1‑151 (MQ=255)
                                                                                           cGACCTTGTGCGTGTTGATGCTCACGATATCTTCAGCCACAGTACAACCAAAATTGGGTTTCGCCGTAGTACTTTCTTGCGCAGTTATATGTATGATTTCATTCAGCGTTTTGCACCGCATTTAACGCGTGATGTCGTTGATGCGGCTGTc                                                     >  2:264313/1‑151 (MQ=255)
                                                                                                 tgtgCGTGTTGATGCTCACGATATCTTCAGCCACAGTACAACCAAAATTGGGTTTCGCCGTAGTACTTTCTTGCGCAGTTATATGTATGATTTCATTCAGCGTTTTGCACCGCATTTAACGCGTGATGTCGTTGATGCGGCTGTCGCATTg                                               >  2:80031/1‑151 (MQ=255)
                                                                                                   tgCGTGTTGATGCTCACGATATCTTCAGCCACAGTACAACCAAAATTGGGTTTCGCCGTAGTACTTTCTTGCGCAGTTATATGTATGATTTCATTCAGCGTTTTGCACCGCATTTAACGCGTGATGTCGTTGATGCGGCTGTCGCATTgcg                                             >  2:234506/1‑151 (MQ=255)
                                                                                                              gCTCACGATATCTTCAGCCACAGTACAACCAAAATTGGGTTTCGCCGTAGTACTTTCTTGCGCAGTTATATGTATGATTTCATTCAGCGTTTTGCACCGCATTTAACGCGTGATGTCGTTGATGCGGCTGTCGCATTGCGCTCTAATgaa                                   <  1:828117/150‑1 (MQ=255)
                                                                                                               cTCACGATATCTTCAACCACAGTACAACCAAAATTGGGTTTCGCCGTAGTACTTTCTTGCGCAGTTATATGTATGATTTCATTCAGCGTTTTGCACCGCATTTAACGCGTGATGTCGTTGATGCGGCTGTCGCATTGCGCTCTAATga                                    >  2:556204/1‑148 (MQ=255)
                                                                                                                tCACGATATCTTCAGCCACAGTACAACCAAAATTGGGTTTCGCCGTAGTACTTTCTTGCGCAGTTATATGTATGATTTCATTCAGCGTTTTGCACCGCATTTAACGCGTGATGTCGTTGATGCGGCTGTCGCATTGCGCTCTAATgaaga                                 >  2:900199/1‑150 (MQ=255)
                                                                                                                tCACGATATCTTCAGCCACAGTACAACCAAAATTGGGTTTCGCCGTAGTACTTTCTTGCGCAGTTATATGTATGATTTCATTCAGCGTTTTGCACCGCATTTAACGCGTGATGTCGTTGATGCGGCTGTCGCATTGCGCTCTAATgaag                                  >  1:314307/1‑149 (MQ=255)
                                                                                                                 cACGATATCTTCAGCCACAGTACAACCAAAATTGGGTTTCGCCGTAGTACTTTCTTGCGCAGTTATATGTATGATTTCATTCAGCGTTTTGCACCGCATTTAACGCGTGATGTCGTTGATGCGGCTGTCGCATTGCGCTCTAATgaagaa                                >  2:514059/1‑150 (MQ=255)
                                                                                                                 cACGATATCTTCAGCCACAGTACAACCAAAATTGGGTTTCGCCGTAGTACTTTCTTGCGCAGTTATATGTATGATTTCATTCAGCGTTTTGCACCGCATTTAACGCGTGATGTCGTTGATGCGGCTGTCGCATTGCGCTCTAATGAAGaaa                               <  2:34449/151‑1 (MQ=255)
                                                                                                                     atatCTTCAGCCACAGTACAACCAAAATTGGGTTTCGCCGTAGTACTTTCTTGCGCAGTTATATGTATGATTTCATTCAGCGTTTTGCACCGCATTTAACGCGTGATGTCGTTGATGCGGCTGTCGCATTGCGCTCTAATGAAGAAATTGa                           <  1:770035/151‑1 (MQ=255)
                                                                                                                         cTTCAGCCACAGTACAACCAAAATTGGGTTTCGCCGTAGTACTTTCTTGCGCAGTTATATGTATGATTTCATTCAGCGTTTTGCACCGCATTTAACGCGTGATGTCGTTGATGCGGCTGTCGCATTGCGCTCTAATGAAGAAATTGAGGTc                       >  2:561638/1‑151 (MQ=255)
                                                                                                                          ttCAGCCACAGTACAACCAAAATTGGGCTTCGCCGTAGTACTTTCTTGCGCAGTTATATGTATGATTTCATTCAGCGTTTTGCACCGCATTTAACGCGTGATGCCGTTGATGCGGCTGTCGCATTGCGCTCTAATGAAGAAATTGAg                          >  2:275316/1‑147 (MQ=255)
                                                                                                                           tCAGCCACAGTACAACCAAAATTGGGTTTCGCCGTAGTACTTTCTTGCGCAGTTATATGTATGATTTCATTCAGCGTTTTGCACCGCATTTAACGCGTGATGTCGTTGATGCGGCTGTCGCATTGCGCTCTAATGAAGAAATTGAGGt                        <  2:207604/148‑1 (MQ=255)
                                                                                                                           tCAGCCACAGTACAACCAAAATTGGGTTTCGCCGTAGTACTTTCTTGCGCAGTTATATGTATGATTTCATTCAGCGTTTTGCACCGCATTTAACGCGTGATGTCGTTGATGCGGCTGTCGCATTGCGCTCTAATGAAGAAATTGAGGTCa                      <  2:580758/150‑1 (MQ=255)
                                                                                                                             aGCCACAGTACAACCAAAATTGGGTTTCGCCGTAGTACTTTCTTGCGCAGTTATATGTATGATTTCATTCAGCGTTTTGCACCGCATTTAACGCGTGATGTCGTTGATGCGGCTGTCGCATTGCGCTCTAATGAAGAAATTGAGGTCATGt                   >  1:915271/1‑151 (MQ=255)
                                                                                                                               ccACAGTACAACCAAAATTGGGTTTCGCCGTAGTACTTTCTTGCGCAGTTATATGTATGATTTCATTCAGCGTTTTGCACCGCATTTAACGCGTGATGTCGTTGATGCGGCTGTCGCATTGCGCTCTAATGAAGAAATTGAGGTCATGttt                 >  2:590686/1‑151 (MQ=255)
                                                                                                                                 acaGTACAACCAAAATTGGGTTTCGCCGTAGTACTTTCTTGCGCAGTTATATGTATGATTTCATTCAGCGTTTTGCACCGCATTTAACGCGTGATGTCGTTGATGCGGCTGTCGCATTGCGCTCTAATGAAGAAATTGAGGTCATGTTTaa               <  2:668517/151‑1 (MQ=255)
                                                                                                                                 acaGTACAACCAAAATTGGGTTTCGCCGTAGTACTTTCTTGCGCAGTTATATGTATGATTTCATTCAGCGTTTTGCACCGCATTTAACGCGTGATGTCGTTGATGCGGCTGTCGCATTGCGCTCTAATGAAGAAATTGAGGTCATGTTTa                >  1:67299/1‑150 (MQ=255)
                                                                                                                                 acaGTACAACCAAAATTGGGTTTCGCCGTAGTACTTTCTTGCGCAGTTATATGTATGATTTCATTCAGCGTTTTGCACCGCATTTAACGCGTGATGTCGTTGATGCGGCTGTCGCATTGCGCTCTAATGAAGAAATTGAGGTCATGTTTa                >  2:536940/1‑150 (MQ=255)
                                                                                                                                  caGTACAACCAAAATTGGGTTTCGCCGTAGTACTTTCTTGCGCAGTTATATGTATGATTTCATTCAGCGTTTTGCACCGCATTTAACGCGTGATGTCGTTGATGCGGCTGTCGCATTGCGCTCTAATGAAGAAATTGAGGTCATGTTTaaa              <  1:564052/151‑1 (MQ=255)
                                                                                                                                           cAAAATTGGTTTTCGCCGTAGTACTTTCTTGCGCAGTTATATGTATGATTTCATTCAGCGTTTTGCACCGCATTTAACGCGTGATGTCGTTGATGCGGCTGTCGCATTGCGCTCTAATGAAGAAATTGAGGTCATGTTTAAAGATATaaa      >  1:41397/1‑150 (MQ=255)
                                                                                                                                           cAAAATTGGGTTTCGCCGTAGTACTTTCTTGCGCAGTTATATGTATGATTTCATTCAGCGTTTTGCACCGCATTTAACGCGTGATGTCGTTGATGCGGCTGTCGCATTGCGCTCTAATGAAGAAATTGAGGTCATGTTTAAAGATATaaaa     <  2:836525/151‑1 (MQ=255)
                                                                                                                                             aaaTTGGGTTTCGCCGTAGTACTTTCTTGCGCAGTTATATGTATGATTTCATTCAGCGTTTTGCACCGCATTTAACGCGTGATGTCGTTGATGCGGCTGTCGCATTGCGCTCTAATGAAGAAATTGAGGTCATGTTTAAAGATATAAAACt   <  2:202172/151‑1 (MQ=255)
                                                                                                                                             aaaTTGGGTTTCGCCGTAGTACTTTCTTGCGCAGTTATATGTATGATTTCATTCAGCGTTTTGCACCGCATTTAACGCGTGATGTCGTTGATGCGGCTGTCGCATTGCGCTCTAATGAAGAAATTGAGGTCATGTTTAAAGATATAAAACt   >  1:236135/1‑151 (MQ=255)
                                                                                                                                              aaTTGGGTTTCGCCGTAGTACTTTCTTGCGCAGTTATATGTATGATTTCATTCAGCGTTTTGCACCGCATTTAACGCGTGATGTCGTTGATGCGGCTGTCGCATTGCGCTCTAATGAAGAAATTGAGGTCATGTTTAAAGATATAAAACTg  >  2:570634/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                
CAACGGATGCTGACGTCATTAAAACTTACGTCCGGTTAGGGCTGGGGGTAGGGGTCATTGCCAGCATGGCGGTGGATCCGGTCGCCGATCCCGACCTTGTGCGTGTTGATGCTCACGATATCTTCAGCCACAGTACAACCAAAATTGGTTTTCGCCGTAGTACTTTCTTGCGCAGTTATATGTATGATTTCATTCAGCGTTTTGCACCGCATTTAACGCGTGATGTCGTTGATGCGGCTGTCGCATTGCGCTCTAATGAAGAAATTGAGGTCATGTTTAAAGATATAAAACTG  >  NC_000913/1334525‑1334817

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑