Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,399,602	T→G	intergenic (‑76/+119)	fnr ← / ← ogt	oxygen‑sensing anaerobic growth regulon transcriptional regulator FNR; autorepressor/O‑6‑alkylguanine‑DNA:cysteine‑protein methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,399,602	0	T	G	100.0%	178.6 / NA	48	intergenic (‑76/+119)	fnr/ogt	oxygen‑sensing anaerobic growth regulon transcriptional regulator FNR; autorepressor/O‑6‑alkylguanine‑DNA:cysteine‑protein methyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (23/25);  total (23/25)

GCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATAGGTCTGCTCAAGCCGTAATTGATATTTGTCAATTTTAACATCTTTTTAGGGAGCAAGTAAGTCTAAGCAAACCTTAACAGCAGAGAATTCCGATATTAGATGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGTTAAGGGTAACTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCAAAAGATAACCTTCATGGCGCA  >  NC_000913/1399455‑1399749                                                                                                                                                    |                                                                                                                                                    gctgcAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATAGGTCTGCTCAAGCCGTAATTGATATTTGTCAATTTTAACATCTTTTTAGGGAGCAAGTAAGTCTAAGCAAACCTGAAc                                                                                                                                                  >  2:714683/1‑151 (MQ=255)   tgcAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATAGGTCTGCTCAAGCCGTAATTGATATTTGTCAATTTTAACATCTTTTTAGGGAGCAAGTAAGTCTAAGCAAACCTGAAc                                                                                                                                                  >  2:43254/1‑149 (MQ=255)            ggCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATAGGTCTGCTCAAGCCGTAATTGATATTTGTCAATTTTAACATCTTTTTAGGGAGCAAGTAAGTCTAAGCAAACCTGAACAGCAGAGAAtt                                                                                                                                       <  1:186822/151‑1 (MQ=255)               aagggAGAGCACACCCGCCAGACTGAATGCGCCGTATAATTCGCTTTACCGGGATCATAGGTCTGCTCAAGCCGTAATTGATATTTGTCAATTTTAACATCTTTTTAGGGAGCAAGTAAGTCTAAGCAAACCTGAACAGCAGAGAATTcc                                                                                                                                     <  1:845668/147‑1 (MQ=255)               aaTGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATAGGTCTGCTCAAGCCGTAATTGATATTTGTCAATTTTAACATCTTTTTAGGGAGCAAGTAAGTCTAAGCAAACCTGAACAGCAGAGAATTcc                                                                                                                                     <  1:318983/150‑1 (MQ=255)               aaTGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATAGGTCTGCTCAAGCCGTAATTGATATTTGTCAATTTTAACATCTTTTTAGGGAGCAAGTAAGTCTAAGCAAACCTGAACAGCAGAGAATTCCg                                                                                                                                    >  1:827219/1‑151 (MQ=255)                  ggATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATAGGTCTGCTCAAGCCGTAATTGATATTTGTCAATTTTAACATCTTTTTAGGGAGCAAGTAAGTCTAAGCAAACCTGAACAGCAGAGAATTCCGata                                                                                                                                 <  2:421270/151‑1 (MQ=255)                        cacaACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATAGGTCTGCTCAAGCCGTAATTGATATTTGTCAATTTTAACATCTTTTTAGGGAGCAAGTAAGTCTAAGCAAACCTGAACAGCAGAGAATTCCGATATTAGAt                                                                                                                           <  1:696529/151‑1 (MQ=255)                         acaACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATAGGTCTGCTCAAGCCGTAATTGATATTTGTCAATTTTAACATCTTTTTAGGGAGCAAGTAAGTCTAAGCAAACCTGAACAGCAGAGAATTCCGATATTAGATg                                                                                                                          >  1:99056/1‑151 (MQ=255)                           aaCCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATAGGTCTGCTCAAGCCGTAATTGATATTTGTCAATTTTAACATCTTTTTAGGGAGCAAGTAAGTCTAAGCAAACCTGAACAGCAGAGAATTCCGATATTAGATGTa                                                                                                                        <  2:623517/151‑1 (MQ=255)                             ccGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATAGGTCTGCTCAAGCCGTAATTGATATTTGTCAATTTTAACATCTTTTTAGGGAGCAAGTAAGTCTAAGCAAACCTGAACAGCAGAGAATTCCGATATTAGATGTaa                                                                                                                       <  2:720536/150‑1 (MQ=255)                              cGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATAGGTCTGCTCAAGCCGTAATTGATATTTGTCAATTTTAACATCTTTTTAGGGAGCAAGTAAGTCTAAGCAAACCTGAACAGCAGAGAATTCCGATATTAGATGTAAat                                                                                                                     <  1:351185/151‑1 (MQ=255)                                    aCTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATAGGTCTGCTCAAGCCGTAATTGATATTTGTCAATTTTAACATCTTTTTAGGGAGCAAGTAAGTCTAAGCAAACCTGAACAGCAGAGAATTCCGATATTAGATGTAAATATATGt                                                                                                               >  2:258564/1‑151 (MQ=255)                                        aaTGCGCCGTATAATTCGCTTTTCCGGGATCATAGGTCTGCTCAAGCCGTAATTGATATTTGTCAATTTTAACATCTTTTTAGGGAGCAAGTAAGTCTAAGCAAACCTGAACAGCAGAGAATTCCGATATTAGATGTAAATATATGtctat                                                                                                           <  2:861279/151‑1 (MQ=255)                                         aTGCGCCGTATAATTCGCTTTTCCGGGATCATAGGTCTGCTCAAGCCGTAATTGATATTTGTCAATTTTAACATCTTTTTAGGGAGCAAGTAAGTCTAAGCAAACCTGAACAGCAGAGAATTCCGATATTAGATGTAAATATATGtctatc                                                                                                          >  1:192476/1‑151 (MQ=255)                                           gcgcCGTATAATTCGCTTTTCCGGGATCATAGGTCTGCTCAAGCCGTAATTGATATTTGTCAATTTTAACATCTTTTTAGGGAGCAAGTAAGTCTAAGCAAACCTGAACAGCAGAGAATTCCGATATTAGATGTAAATATATGtctatct                                                                                                         >  2:773360/1‑150 (MQ=255)                                            cgcCGTATAATTCGCTTTTCCGGGATCATAGGTCTGCTCAAGCCGTAATTGATATTTGTCAATTTTAACATCTTTTTAGGGAGCAAGTAAGTCTAAGCAAACCTGAACAGCAGAGAATTCCGATATTAGATGTAAATATATGTctatctat                                                                                                       >  2:95345/1‑151 (MQ=255)                                                     aTTCGCTTTTCCGGGATCATAGGTCTGCTCAAGCCGTAATTGATATTTGTCAATTTTAACATCTTTTTAGGGAGCAAGTAAGTCTAAGCAAACCTGAACAGCAGAGAATTCCGATATTAGATGTAAATATATGTCTATCTATTTGAAAAcc                                                                                              <  2:777364/151‑1 (MQ=255)                                                       tCGCTTTTCCGGGATCATAGGTCTGCTCAAGCCGTAATTGATATTTGTCAATTTTAACATCTTTTTAGGGAGCAAGTAAGTCTAAGCAAACCTGAACAGCAGAGAATTCCGATATTAGATGTAAATATATGTCTATCTATTTGAAAACCCt                                                                                            >  2:861548/1‑151 (MQ=255)                                                         gCTTTTCCGGGATCATAGGTCTGCTCAAGCCGTAATTGATATTTGTCAATTTTAACATCTTTTTAGGGAGCAAGTAAGTCTAAGCAAACCTGAACAGCAGAGAATTCCGATATTAGATGTAAATATATGTCTATCTATTTGAAAACCCtt                                                                                           >  1:29557/1‑150 (MQ=255)                                                         gCTTTTCCGGGATCATAGGTCTGCTCAAGCCGTAATTGATATTTGTCAATTTTAACATCTTTTTAGGGAGCAAGTAAGTCTAAGCAAACCTGAACAGCAGAGAATTCCGATATTAGATGTAAATATATGTCTATCTATTTGAAAACCCTTa                                                                                          <  2:897634/151‑1 (MQ=255)                                                          cTTTTCCGGGATCATAGGTCTGCTCAAGCCGTAATTGATATTTGTCAATTTTAACATCTTTTTAGGGAGCAAGTAAGTCTAAGCAAACCTGAACAGCAGAGAATTCCGATATTAGATGTAAATATATGTCTATCTATTTGAAAACCCTTa                                                                                          >  1:450331/1‑150 (MQ=255)                                                              tCCGGGATCATAGGTCTGCTCAAGCCGTAATTGATATTTGTCAATTTTAACATCTTTTTAGGGAGCAAGTAAGTCTAAGCAAACCTGAACAGCAGAGAATTCCGATATTAGATGTAAATATATGTCTATCTATTTGAAAACCCTTAAgtt                                                                                      <  2:883353/150‑1 (MQ=255)                                                                cGGGATCATAGGTCTGCTCAAGCCGTAATTGATATTTGTCAATTTTAACATCTTTTTAGGGAGCAAGTAAGTCTAAGCAAACCTGAACAGCAGAGAATTCCGATATTAGATGTAAATATATGTCTATCTATTTGAAAACCCTTAAgttgtt                                                                                   <  1:438179/151‑1 (MQ=255)                                                                  ggATCATAGGTCTGCTCAAGCCGTAATTGATATTTGTCAATTTTAACATCTTTTTAGGGAGCAAGTAAGTCTAAGCAAACCTGAACAGCAGAGAATTCCGATATTAGATGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGTTa                                                                                  >  2:512628/1‑150 (MQ=255)                                                                    aTCATAGGTCTGCTCAAGCCGTTATTGATATTTGTCAATTTTAACATCTTTTTAGGGAGCAAGTAAGTCTAAGCAAACCTGAACAGCAGAGAATTCCGATATTAGATGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGTTAAgg                                                                               <  1:350542/151‑1 (MQ=255)                                                                        tAGGTCTGCTCAAGCCGTAATTGATATTTGTCAATTTTAACATCTTTTTAGGGAGCAAGTAAGTCTAAGCAAACCTGAACAGCAGAGAATTCCGATATTAGATGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGTTAAGGGTa                                                                            >  1:817595/1‑150 (MQ=255)                                                                              tGCTCAAGCCGTAATTGATATTTGTCAATTTTAACATCTTTTTAGGGAGCAAGTAAGTCTAAGCAAACCTGAACAGCAGAGAATTCCGATATTAGATGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGTTAAGGGTAACTTTAc                                                                     >  2:628618/1‑151 (MQ=255)                                                                                 tCAAGCCGTAATTGATATTTGTCAATTTTAACATCTTTTTAGGGAGCAAGTAAGTCTAAGCAAACCTGAACAGCAGAGAATTCCGATATTAGATGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGTTAAGGGTAACTTTACAt                                                                   >  1:336822/1‑150 (MQ=255)                                                                                          aaTTGATATTTGTCAATTTTAACATCTTTTTAGGGAGCAAGTAAGTCTAAGCAAACCTGAACAGCAGAGAATTCCGATATTAGATGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGTTAAGGGTAACTTTACATAAAAGTGTGa                                                         <  1:603050/151‑1 (MQ=255)                                                                                              gATATTTGTCAATTTTAACATCTTTTTAGGGAGCAAGTAAGTCTAAGCAAACCTGAACAGCAGAGAATTCCGATATTAGATGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGTTAAGGGTAACTTTACATAAAAGTGTGAACaa                                                     <  2:386494/151‑1 (MQ=255)                                                                                               atatTTGTCAATTTTAACATCTTTTTAGGGAGCAAGTAAGTCTAAGCAAACCTGAACAGCAGAGAATTCCGATATTAGATGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGTTAAGGGTAACTTTACATAAAAGTGTGAACa                                                      >  2:606875/1‑149 (MQ=255)                                                                                                   ttGTCAATTTTAACATCTTTTTAGGGAGCAAGTAAGTCTAAGCAAACCTGAACAGCAGAGAATTCCGATATTAGATGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGTTAAGGGTAACTTTACATAAAAGTGTGAACAAGCTgg                                                >  1:864512/1‑151 (MQ=255)                                                                                                   ttGTCAATTTTAACATCTTTTTAGGGAGCAAGTAAGTCTAAGCAAACCTGAACAGCAGAGAATTCCGATATTAGATGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGTTAAGGGTAACTTTACATAAAAGTGTGAACAAGCTg                                                 <  1:365000/150‑1 (MQ=255)                                                                                                                cATCTTTTTAGGGAGCAAGTAAGTCTAAGCAAACCTGAACAGCAGAGAATTCCGATATTAGATGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGTTAAGGGTAACTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGttt                                    >  1:377738/1‑150 (MQ=255)                                                                                                                   cTTTTTAGGGAGCAAGTAAGTCTAAGCAAACCTGAACAGCAGAGAATTCCGATATTAGATGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGTTAAGGGTAACTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATg                                >  2:655340/1‑151 (MQ=255)                                                                                                                     ttttAGGGAGCAAGTAAGTCTAAGCAAACCTGAACAGCAGAGAATTCCGATATTAGATGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGTTAAGGGTAACTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGt                               <  1:43252/150‑1 (MQ=255)                                                                                                                      tttAGGGAGCAAGTAAGTCTAAGCAAACCTGAACAGCAGAGAATTCCGATATTAGATGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGTTAAGGGTAACTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGttt                             >  1:28639/1‑151 (MQ=255)                                                                                                                            gAGCAAGTAAGTCTAAGCAAACCTGAACAGCAGAGAATTCCGATATTAGATGTAAATATATGTCTATCTATTTGAAAGCCCTTAAGTTGTTAAGGGTAACTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCa                       <  2:757131/151‑1 (MQ=255)                                                                                                                              gCAAGTAAGTCTAAGCAAACCTGAACAGCAGAGAATTCCGATATTAGATGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGTTAAGGGTAACTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCaa                      <  1:544484/150‑1 (MQ=255)                                                                                                                               cAAGTAAGTCTAAGCAAACCTGAACAGCAGAGAATTCCGATATTAGATGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGTTAAGGGTAACTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCaaaa                    <  2:661846/151‑1 (MQ=255)                                                                                                                                      gtatAAGCAAACCTGAACCGCAGAGAATTCCGATATTAGATGTAAATATATGTCTATCTATTTAAAAACCCTTAAGTTGTTAAGGGTAATTTTACATAAAAGTGTGACCAAGCTGGCAAAAATTGTTTAATGATTACAGCAAAAGATaa               >  1:378527/4‑149 (MQ=255)                                                                                                                                        cTAAGCAAACCTGAACAGCAGAGAATTCCGATATTAGATGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGTTAAGGGTAACTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCAAAAGATAACCtt           >  1:495550/1‑151 (MQ=255)                                                                                                                                          aaGCAAACCTGAACAGCAGAGAATTCCGATATTAGATGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGTTAAGGGTAACTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCAAAAGATAACCTTc          <  1:880815/150‑1 (MQ=255)                                                                                                                                          aaGCAAACCTGAACAGCAGAGAATTCCGATATTAGATGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGTTAAGGGTAACTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCAAAAGATAACCTTc          >  2:407991/1‑150 (MQ=255)                                                                                                                                          aaGCAAACCTGAACAGCAGAGAATTCCGATATTAGATGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGTTAAGGGTAACTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCAAAAGATAACCTTc          <  2:548133/150‑1 (MQ=255)                                                                                                                                             cAAACCTGAACAGCAGAGAATTCCGATATTAGATGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGTTAAGGGTAACTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCAAAAGATAACCTTCATgg      <  2:381494/151‑1 (MQ=255)                                                                                                                                                 ccTGAACAGCAGAGAATTCCGATATTAGATGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGTTAAGGGTAACTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCAAAAGATAACCTTCATGGCGCa  <  2:892835/151‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                    GCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATAGGTCTGCTCAAGCCGTAATTGATATTTGTCAATTTTAACATCTTTTTAGGGAGCAAGTAAGTCTAAGCAAACCTTAACAGCAGAGAATTCCGATATTAGATGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGTTAAGGGTAACTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCAAAAGATAACCTTCATGGCGCA  >  NC_000913/1399455‑1399749

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑