Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	83,522	T→G	Q3P (CAG→CCG)	leuA ←	2‑isopropylmalate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	83,522	0	T	G	100.0%	197.7 / NA	58	Q3P (CAG→CCG)	leuA	2‑isopropylmalate synthase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (29/29);  total (29/29)

AGACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGT  >  NC_000913/83382‑83668                                                                                                                                             |                                                                                                                                                   agaCGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCGGGCTCATGGt                                                                                                                                          >  2:680741/1‑151 (MQ=255)   aCGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCGGGCTCATGGttt                                                                                                                                        <  1:901497/151‑1 (MQ=255)      gggAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCGGGCTCATGGTTTggg                                                                                                                                     <  1:870578/151‑1 (MQ=255)         aaaCCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCGGGCTCATGGTTTGGGTcc                                                                                                                                  >  1:664676/1‑151 (MQ=255)             cGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCGGGCTCATGGTTTGGGTCCTTGt                                                                                                                              <  2:92119/151‑1 (MQ=255)             cGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCGGGCTCATGGTTTGGGTCCTTGt                                                                                                                              <  2:652401/151‑1 (MQ=255)              gACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCGGGCTCATGGTTTGGGTCCTTGtc                                                                                                                             >  2:921900/1‑151 (MQ=255)                   ccATAACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCATGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCGGGCTCATGGTTTGGGACCTTGTCTCttt                                                                                                                        >  2:604118/1‑151 (MQ=255)                    cATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCGGGCTCATGGTTTGGGTCCTTGTCTCtttt                                                                                                                       >  1:498979/1‑151 (MQ=255)                     aTCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCGGGCTCATGGTTTGGGTCCTTGTCTCTTTTa                                                                                                                      <  2:882487/151‑1 (MQ=255)                          gTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCGGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAgcg                                                                                                                 <  2:686522/151‑1 (MQ=255)                             aaCACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCGGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGcc                                                                                                               >  1:731127/1‑150 (MQ=255)                                 cccATACGCTCAAGGGCCAGTGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCGGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCt                                                                                                          <  2:638996/151‑1 (MQ=255)                                 cccATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCGGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCt                                                                                                          <  2:28826/151‑1 (MQ=255)                                  ccATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCGGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCtt                                                                                                         >  2:865330/1‑151 (MQ=255)                                  ccATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCGGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCtt                                                                                                         >  1:856424/1‑151 (MQ=255)                                    aTACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCGGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCg                                                                                                       >  2:300082/1‑151 (MQ=255)                                    aTACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCGGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCg                                                                                                       <  1:381932/151‑1 (MQ=255)                                     tACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCGGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCgg                                                                                                      >  1:138055/1‑151 (MQ=255)                                              gggCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCGGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATaaaaa                                                                                             <  1:233586/151‑1 (MQ=255)                                                     cgcAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCGGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCcgc                                                                                      <  1:402306/151‑1 (MQ=255)                                                     cgcAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCGGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCcgc                                                                                      <  1:651818/151‑1 (MQ=255)                                                      gcAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCGGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCcgcg                                                                                     >  2:541485/1‑151 (MQ=255)                                                        aaTTTGCAGTTTTTCTTTCACACTCAAGCTTGCATGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCGGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCa                                                                                   <  2:728927/151‑1 (MQ=255)                                                         aTTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCGGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCaa                                                                                  <  2:693065/151‑1 (MQ=255)                                                              cAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCGGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGgcg                                                                             <  2:505910/151‑1 (MQ=255)                                                                      ctttcACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCGGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGtt                                                                       <  1:307831/149‑1 (MQ=255)                                                                        ttcACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCGGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGtttttt                                                                   >  2:575204/1‑151 (MQ=255)                                                                           acacTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCGGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTtttgtt                                                                <  2:629235/151‑1 (MQ=255)                                                                            cacTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCGGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTtttgttt                                                               >  2:125401/1‑151 (MQ=255)                                                                              cTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCGGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTtttgtttg                                                              >  2:870060/1‑150 (MQ=255)                                                                                cAAGCTTGCTTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCGGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACt                                                           >  2:860588/1‑151 (MQ=255)                                                                                cAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCGGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACt                                                           >  1:883583/1‑151 (MQ=255)                                                                                cAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCGGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACt                                                           >  1:869666/1‑151 (MQ=255)                                                                                 aaGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCGGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTg                                                          <  2:137534/151‑1 (MQ=255)                                                                                     ttGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCGGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTg                                                      >  1:286643/1‑151 (MQ=255)                                                                                      tGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCGGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTg                                                      <  1:903819/150‑1 (MQ=255)                                                                                            tAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCGGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCtt                                               >  2:106096/1‑151 (MQ=255)                                                                                               cGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCGGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGc                                                 <  1:21968/146‑1 (MQ=255)                                                                                               cGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCGGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAAt                                            <  2:100856/151‑1 (MQ=255)                                                                                                gCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCGGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATg                                           >  2:747188/1‑151 (MQ=255)                                                                                                         ccGTCGCGCAATGTGGTATCGAAAATAATGACTTGCGGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATg                                    <  1:669623/149‑1 (MQ=255)                                                                                                           gTCGCGCAATGTGGTATCGAAAATAATGACTTGCGGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGc                                <  1:398292/151‑1 (MQ=255)                                                                                                               cgcAATGTGGTATCGAAAATAATGACTTGCGGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCAc                            >  2:497017/1‑151 (MQ=255)                                                                                                                gcAATGTGGTATCGAAAATAATGACTTGCGGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACt                           >  1:118342/1‑151 (MQ=255)                                                                                                                 cAATGTGGTATCGAAAATAATGACTTGCGGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTc                          >  1:389127/1‑151 (MQ=255)                                                                                                                   aTGTGGTATCGAAAATAATGACTTGCGGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTc                          <  1:79376/149‑1 (MQ=255)                                                                                                                   aTGTGGTATCGAAAATAATGACTTGCGGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGt                        <  2:685277/151‑1 (MQ=255)                                                                                                                   aTGTGGTATCGAAAATAATGACTTGCGGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGt                        >  1:298486/1‑151 (MQ=255)                                                                                                                   aTGTGGTATCGAAAATAATGACTTGCGGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGt                        >  2:773925/1‑151 (MQ=255)                                                                                                                            cGAAAATAATGACTTGCGGGTTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACcgcg                <  1:201341/150‑1 (MQ=255)                                                                                                                                aataatGACTTGCGGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCaa             >  1:893127/1‑149 (MQ=255)                                                                                                                                   aatGACTTGCGGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGTCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAaagaag        <  1:763451/151‑1 (MQ=255)                                                                                                                                   aatGACTTGCGGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAaagaag        <  2:842060/151‑1 (MQ=255)                                                                                                                                   aatGACTTGCGGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAaaga          >  2:29081/1‑149 (MQ=255)                                                                                                                                      gACTTGCGGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATg     >  2:180267/1‑151 (MQ=255)                                                                                                                                        cTTGCGGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGAt      <  1:67318/148‑1 (MQ=255)                                                                                                                                         ttGCGGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGt  >  1:165377/1‑151 (MQ=255)                                                                                                                                             |                                                                                                                                                   AGACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGT  >  NC_000913/83382‑83668

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑