Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,404,625	A→C	intergenic (‑60/‑116)	abgA ← / → abgR	p‑aminobenzoyl‑glutamate hydrolase, A subunit/putative DNA‑binding transcriptional regulator of abgABT operon

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,404,625	0	A	C	100.0%	198.8 / NA	53	intergenic (‑60/‑116)	abgA/abgR	p‑aminobenzoyl‑glutamate hydrolase, A subunit/putative DNA‑binding transcriptional regulator of abgABT operon
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (28/25);  total (28/25)

CAGCCAGACTCTGCATAGTGATGAAAATCACGTCGCCAGTGCGATAATTTTGGGGCAAGCGAATTAACAAATTGATTCAAAGACTCCATAACCTTTCCCGTCATCAGTAAAAAGTGTGACCCGGTTCACGTAGCGATAGTTTTTACTTATCACTAACTGATTTTTCACAGTTTTAACCGTTCATAAATTACCCTGACACAATCATCTGCATTAAAGTAGATGCCAGTTTCTTTGGTCTGATAAATAACGGTTATCGGTGGCGTCATGGCTTTTCAGGTAAAAATTCATCAAATTC  >  NC_000913/1404477‑1404771                                                                                                                                                     |                                                                                                                                                   cagccagACTCTGCATAGTGATGAAAATCACGTCGCCAGTGCGATAATTTTGGGGCAAGCGAATTAACAAATTGATTCAAAGACTCCATAACCTTTCCCGTCATCAGTAAAAAGTGTGACCCGGTTCACGTAGCGATAGTTTTTACTtctc                                                                                                                                                  <  1:923069/151‑1 (MQ=255)     cagACTCTGCATAGTGATGAAAATCACGTCGCCAGTGCGATAATTTTGGGGCAAGCGAATTAACAAATTGATTCAAAGACTCCATAACCTTTCCCGTCATCAGTAAAAAGTGTGACCCGGTTCACGTAGCGATAGTTTTTACTTCTCACTa                                                                                                                                              <  1:168493/151‑1 (MQ=255)     cagACTCTGCATAGTGATGAAAATCACGTCGCCAGTGCGATAATTTTGGGGCAAGCGAATTAACAAATTGATTCAAAGACTCCATAACCTTTCCCGTCATCAGTAAAAAGTGTGACCCGGTTCACGTAGCGATAGTTTTTACTTCTCACTa                                                                                                                                              <  1:749724/151‑1 (MQ=255)        aCTCTGCATAGTGATGAAAATCACGTCGCCAGTGCGATAATTTTGGGGCAAGCGAATTAACAAATTGATTCAAAGACTCCATAACCTTTCCCGTCATCAGTAAAAAGTGTGACCCGGTTCACGTAGCGATAGTTTTTACTTCTCACTAACt                                                                                                                                           >  1:399306/1‑151 (MQ=255)         ctctGCATAGTGATGAAAATCACGTCGCCAGTGCGATAATTTTGGGGCAAGCGAATTAACAAATTGATTCAAAGACTCCATAACCTTTCCCGTCATCAGTAAAAAGTGTGACCCGGTTCACGTAGCGATAGTTTTTACTTCTCACTAACTg                                                                                                                                          >  1:417314/1‑151 (MQ=255)               aTAGTGATGAAAATCACGTCGCCAGTGCGATAATTTTGGGGCAAGCGAATTAACAAATTGATTCAAAGACTCCATAACCTTTCCCGTCATCAGTAAAAAGTGTGACCCGGTTCACGTAGCGATAGTTTTTACTTCTCACTAACTGAttttt                                                                                                                                    >  2:928211/1‑151 (MQ=255)                 aGTGATGAAAATCACGTCGCCAGTGCGATAATTTTGGGGCAAGCGAATTAACAAATTGATTCAAAGACTCCATAACCTTTCCCGTCATCAGTAAAAAGTGTGACCCGGTTCACGTAGCGATAGTTTTTACTTCTCACTAACTGATTTTTca                                                                                                                                  <  1:468478/151‑1 (MQ=255)                   tgatgaAAATCACGTCGCCAGTGCGATAATTTTGGGGCAAGCGAATTAACAAATTGATTCAAAGACTCCATAACCTTTCCCGTCATCAGTAAAAAGTGTGACCCGGTTCACGTAGCGATAGTTTTTACTTCTCACTAACTGATTTTTcac                                                                                                                                 >  2:926200/1‑150 (MQ=255)                    gatgaAAATCACGTCGCCAGTGCGATAATTTTGGGGCAAGCGAATTAACAAATTGATTCAAAGACTCCATAACCTTTCCCGTCATCAGTAAAAAGTGTGACCCGGTTCACGTAGCGATAGTTTTTACTTCTCACTAACTGATTTTTcaca                                                                                                                                <  2:861661/150‑1 (MQ=255)                             cACGTCGCCAGTGCGATAATTTTGGTGCAAGCGAATTAACAAATTGATTCAAAGACTCCATAACCTTTCCCGTCATCAGTAAAAAGTGTGACCCGGTTCACGTAGCGATAGTTTTTACTTCTCACTAACTGATTTTTCACAGTTTTAACCg                                                                                                                      <  1:925922/151‑1 (MQ=255)                              aCGTCGCCAGTGCGATAATTTTGGGGCAAGCGAATTAACAAATTGATTCAAAGACTCCATAACCTTTCCCGTCATCAGTAAAAAGTGTGACCCGGTTCACGTAGCGATAGTTTTTACTTCTCACTAACTGATTTTTCACAGTTTTAACCGt                                                                                                                     <  1:161188/151‑1 (MQ=255)                                  cGCCAGTGCGATAATTTTGGGGCAAGCGAATTAACAAATTGATTCAAAGACTCCATAACCTTTCCCGTCATCAGTAAAAAGTGTGACCCGGTTCACGTAGCGATAGTTTTTACTTCTCACTAACTGATTTTTCACAGTTTTAACCGTTCa                                                                                                                  <  1:458203/150‑1 (MQ=255)                                   gCCAGTGCGATAATTTTGGGGCAAGCGAATTAACAAATTGATTCAAAGACTCCATAACCTTTCCCGTCATCAGTAAAAAGTGTGACCCGGTTCACGTAGCGATAGTTTTTACTTCTCACTAACTGATTTTTCACAGTTTTAACCGTTCATa                                                                                                                <  1:277642/151‑1 (MQ=255)                                     cAGTGCGATAATTTTGGGGCAAGCGAATTAACAAATTGATTCAAAGACTCCATAACCTTTCCCGTCATCAGTAAAAAGTGTGACCCGGTTCACGTAGCGATAGTTTTTACTTCTCACTAACTGATTTTTCACAGTTTTAACCGTTCATaa                                                                                                               <  1:551969/150‑1 (MQ=255)                                       gTGCGATAATTTTGGGGCAAGCGAATTAACAAATTGATTCAAAGACTCCATAACCTTTCCCGTCATCAGTAAAAAGTGTGACCCGGTTCACGTAGCGATAGTTTTTACTTCTCACTAACTGATTTTTCACAGTTTTAACCGTTCATAAAtt                                                                                                            <  2:893157/151‑1 (MQ=255)                                          cGATAATTTTGGGGCAAGCGAATTAACAAATTGATTCAAAGACTCCATAACCTTTCCCGTCATCAGTAAAAAGTGTGACCCGGTTCACGTAGCGATAGTTTTTACTTCTCACTAACTGATTTTTCACAGTTTTAACCGTTCATAAATTAcc                                                                                                         >  2:519894/1‑151 (MQ=255)                                           gATAATTTTGGGGCAAGCGAATTAACAAATTGATTCAAAGACTCCATAACCTTTCCCGTCATCAGTAAAAAGTGTGACCCGGTTCACGTAGCGATAGTTTTTACTTCTCACTAACTGATTTTTCACAGTTTTAACCGTTCATAAATTAccc                                                                                                        >  1:671494/1‑151 (MQ=255)                                           gATAATTTTGGGGCAAGCGAATTAACAAATTGATTCAAAGACTCCATAACCTTTCCCGTCATCAGTAAAAAGTGTGACCCGGTTCACGTAGCGATAGTTTTTACTTCTCACTAACTGATTTTTCACAGTTTTAACCGTTCATAAATTAccc                                                                                                        >  1:284894/1‑151 (MQ=255)                                              aaTTTTGGGGCAAGCGAATTAACAAATTGATTCAAAGACTCCATAACCTTTCCCGTCATCAGTAAAAAGTGTGACCCGGTTCACGTAGCGATAGTTTTTACTTCTCACTAACTGATTTTTCACAGTTTTAACCGTTCATAAATTACCCTGa                                                                                                     <  2:929038/151‑1 (MQ=255)                                                     gggCAAGCGAATTAACAAATTGATTCAAAGACTCCATAACCTTTCCCGTCATCAGTAAAAAGTGTGACCCGGTTCACGTAGCGATAGTTTTTACTTCTCACTAACTGATTTTTCACAGTTTTAACCGTTCATAAATTACCCTGACACAatc                                                                                              >  1:906769/1‑151 (MQ=255)                                                         aaGCGAATTAACAAATTGATTCAAAGACTCCATAACCTTTCCCGTCATCAGTAAAAAGTGTGACCCGGTTCACGTAGCGATAGTTTTTACTTCTCACTAACTGATTTTTCACAGTTTTAACCGTTCATAAATTACCCTGACACAATCATCt                                                                                          >  1:29898/1‑151 (MQ=255)                                                          aGCGAATTAACAAATTGATTCAAAGACTCCATAACCTTTCCCGTCATCAGTAAAAAGTGTGACCCGGTTCACGTAGCGATAGTTTTTACTTCTCACTAACTGATTTTTCACAGTTTTAACCGTTCATAAATTACCCTGACACAATCATCt                                                                                          >  2:613628/1‑150 (MQ=255)                                                          aGCGAATTAACAAATTGATTCAAAGACTCCATAACCTTTCCCGTCATCAGTAAAAAGTGTGACCCGGTTCACGTAGCGATAGTTTTTACTTCTCACTAACTGATTTTTCACAGTTTTAACCGTTCATAAATTACCCTGACACAATCATCTg                                                                                         <  1:659775/151‑1 (MQ=255)                                                             gAATTAACAAATTGATTCAAAGACTCCATAACCTTTCCCGTCATCAGTAAAAAGTGTGACCCGGTTCACGTAGCGATAGTTTTTACTTCTCACTAACTGATTTTTCACAGTTTTAACCGTTCATAAATTACCCTGACACAATCATCTGCAt                                                                                      <  2:479014/151‑1 (MQ=255)                                                              aaTTAACAAATTGATTCAAAGACTCCATAACCTTTCCCGTCATCAGTAAAAAGTGTGACCCGGTTCACGTAGCGATAGTTTTTACTTCTCACTAACTGATTTTTCACAGTTTTAACCGTTCATAAATTACCCTGACACAATCATCTGCAtt                                                                                     >  1:657599/1‑151 (MQ=255)                                                              aaTTAACAAATTGATTCAAAGACTCCATAACCTTTCCCGTCATCAGTAAAAAGTGTGACCCGGTTCACGTAGCGATAGTTTTTACTTCTCACTAACTGATTTTTCACAGTTTTAACCGTTCATAAATTACCCTGACACAATCATCTGCAt                                                                                      >  2:54608/1‑150 (MQ=255)                                                               aTTAACAAATTGATTCAAAGACTCCATAACCTTTCCCGTCATCAGTAAAAAGTGTGACCCGGTTCACGTAGCGATAGTTTTTACTTCTCACTAACTGATTTTTCACAGTTTTAACCGTTCATAAATTACCCTGACACAATCATCTGCATTa                                                                                    <  2:14862/151‑1 (MQ=255)                                                                  aaCAAATTGATTCAAAGACTCCATAACCTTTCCCGTCATCAGTAAAAAGTGTGACCCGGTTCACGTAGCGATAGTTTTTACTTCTCACTAACTGATTTTTCACAGTTTTAACCGTTCATAAATTACCCTGACACAATCATCTGCATTAAAg                                                                                 <  1:100346/151‑1 (MQ=255)                                                                             tCAAAGACTCCATAACCTTTCCCGTCATCAGTAAAAAGTGTGACCCGGTTCACGTAGCGATAGTTTTTACTTCTCACTAACTGATTTTTCACAGTTTTAACCGTTCATAAATTACCCTGACACAATCATCTGCATTAAAGTAGATGCCAGt                                                                      >  2:268250/1‑151 (MQ=255)                                                                              cAAAGACTCCATAACCTTTCCCGTCATCAGTAAAAAGTGTGACCCGGTTCACGTAGCGATAGTTTTTACTTCTCACTAACTGATTTTTCACAGTTTTAACCGTTCATAAATTACCCTGACACAATCATCTGCATTAAAGTAGATGCCAGtt                                                                     <  2:341646/151‑1 (MQ=255)                                                                               aaaGACTCCATAACCTTTCCCGTCATCAGTAAAAAGTGTGACCCGGTTCACGTAGCGATAGTTTTTACTTCTCACTAACTGATTTTTCACAGTTTTAACCGTTCATAAATTACCCTGACACAATCATCTGCATTAAAGTAGATGCCAGttt                                                                    >  2:335553/1‑151 (MQ=255)                                                                                      ccATAACCTTTCCCGTCATCAGTAAAAAGTGTGACCCGGTTCACGTAGCGATAGTTTTTACTTCTCACTAACTGATTTTTCACAGTTTTAACCGTTCATAAATTACCCTGACACAATCATCTGCATTAAAGTAGATGCCAGTTTCTTTg                                                               <  1:887089/149‑1 (MQ=255)                                                                                      ccATAACCTTTCCCGTCATCAGTAAAAAGTGTGACCCGGTTCACGTAGCGATAGTTTTTACTTCTCACTAACTGATTTTTCACAGTTTTAACCGTTCATAAATTACCCTGACACAATCATCTGCATTAAAGTAGATGCCAGTTTCTTTGGt                                                             >  1:393023/1‑151 (MQ=255)                                                                                           aCCTTTCCCGTCATCAGTAAAAAGTGTGACCCGGTTCACGTAGCGATAGTTTTTACTTCTCACTAACTGATTTTTCACAGTTTTAACCGTTCATAAATTACCCTGACACAATCATCTGCATTAAAGTAGATGCCAGTTTCTTTGGTCTGat                                                        >  2:287528/1‑151 (MQ=255)                                                                                            ccTTTCCCGTCATCAGTAAAAAGTGTGACCCGGTTCACGTAGCGATAGTTTTTACTTCTCACTAACTGATTTTTCACAGTTTTAACCGTTCATAAATTACCCTGACACAATCATCTGCATTAAAGTAGATGCCAGTTTCTTTGGTCTGat                                                        >  2:60863/1‑150 (MQ=255)                                                                                            ccTTTCCCGTCATCAGTAAAAAGTGTGACCCGGTTCACGTAGCGATAGTTTTTACTTCTCACTAACTGATTTTTCACAGTTTTAACCGTTCATAAATTACCCTGACACAATCATCTGCATTAAAGTAGATGCCAGTTTCTTTGGTCTGat                                                        >  2:145456/1‑150 (MQ=255)                                                                                               ttCCCGTCATCAGTAAAAAGTGTGACCCGGTTCACGTAGCGATAGTTTTTACTTCTCACTAACTGATTTTTCACAGTTTTAACCGTTCATAAATTACCCTGACACAATCATCTGCATTAAAGTAGATGCCAGTTTCTTTGGTCTGataaat                                                    >  2:298146/1‑151 (MQ=255)                                                                                                tCCCGTCATCAGTAAAAAGTGTGACCCGGTTCACGTAGCGATAGTTTTTACTTCTCACTAACTGATTTTTCACAGTTTTAACCGTTCATAAATTACCCTGACACAATCATCTGCATTAAAGTAGATGCCAGTTTCTTTGGTCTGataaat                                                    <  1:395317/150‑1 (MQ=255)                                                                                                      catcaGTAAAAAGTGTGACCCGGTTCACGTAGCGATAGTTTTTACTTCTCACTAACTGATTTTTCACAGTTTTAACCGTTCATAAATTTCCCTTACACAATCATCTGCATTAAAGTAGACGCCAGTTTCTTTGGTCTGATAAATGACg                                                <  1:38083/148‑1 (MQ=255)                                                                                                        tcaGTAAAAAGTGTGACCCGGTTCACGTAGCGATAGTTTTTACTTCTCACTAACTGATTTTTCACAGTTTTAACCGTTCATAAATTACCCTGACACAATCATCTGCATTAAAGTAGATGCCAGTTTCTTTGGTCTGATAAATAACGGTTAt                                           >  2:218372/1‑151 (MQ=255)                                                                                                           gTAAAAAGTGTGACCCGGTTCACGTAGCGATAGTTTTTACTTCTCACTAACTGATTTTTCACAGTTTTAACCGTTCATAAATTACCCTGACACAATCATCTGCATTAAAGTAGATGCCAGTTTCTTTGGTCTGATAAATAACGGTTATCgg                                        >  1:9415/1‑151 (MQ=255)                                                                                                            tAAAAAGTGTGACCCGGTTCACGTAGCGATAGTTTTTACTTCTCACTAACTGATTTTTCACAGTTTTAACCGTTCATAAATTACCCTGACACAATCATCTGCATTAAAGTAGATGCCAGTTTCTTTGGTCTGATAAATAACGGTTATCGGt                                       >  1:393216/1‑151 (MQ=255)                                                                                                                aaGTGTGACCCGGTTCACGTAGCGATAGTTTTTACTTCTCACTAACTGATTTTTCACAGTTTTAACCGTTCATAAATTACCCTGACACAATCATCTGCATTAAAGTAGATGCCAGTTTCTTTGGTCTGATAAATAGCGGTTATCGGTGGCg                                   >  1:266294/1‑151 (MQ=255)                                                                                                                     tgACCCGGTTCACGTAGCGATAGTTTTTACTTCTCACTAACTGATTTTTCACAGTTTTAACCGTTCATAAATTACCCTGACACAATCATCTGCATTAAAGTAGATGCCAGTTTCTTTGGTCTGATAAATAACGGTTATCGGTGGCGTCATg                              >  1:252929/1‑151 (MQ=255)                                                                                                                           ggTTCACGTAGCGATAGTTTTTACTTCTCACTAACTGATTTTTCACAGTTTTAACCGTTCATAAATTACCCTGACACAATCATCTGCATTAAAGTAGATGCCAGTTTCTTTGGTCTGATAAATAACGGTTATCGGTGGCGTCATGGCtttt                        <  1:109284/151‑1 (MQ=255)                                                                                                                           ggTTCACGTAGCGATAGTTTTTACTTCTCACTAACTGATTTTTCACAGTTTTAACCGTTCATAAATTACCCTGACACAATCATCTGCATTAAAGTAGATGCCAGTTTCTTTGGTCTGATAAATAACGGTTATCGGTGGCGTCATGGCttt                         <  1:719730/150‑1 (MQ=255)                                                                                                                               cACGTAGCGATAGTTTTTACTTCTCACTAACTGATTTTTCACAGTTTTAACCGTTCATAAATTACCCTGACACAATCATCTGCATTAAAGTAGATGCCAGTTTCTTTGGTCTGATAAATAACGGTTATCGGTGGCGTCATGGCTTTTCAg                     <  1:888953/150‑1 (MQ=255)                                                                                                                                aCGTAGCGATAGTTTTTACTTCTCACTAACTGATTTTTCACAGTTTTAACCGTTCATAAATTACCCTGACACAATCATCTGCATTAAAGTAGATGCCAGTTTCTTTGGTCTGATAAATAACGGTTATCGGTGGCGTCATGGCTTTTCAGGt                   >  2:821763/1‑151 (MQ=255)                                                                                                                                    aGCGATAGTTTTTACTTCTCACTAACTGATTTTTCACAGTTTTAACCGTTCATAAATTACCCTGACACAATCATCTGCATTAAAGTAGATGCCAGTTTCTTTGGTCTGATAAATAACGGTTATCGGTGGCGTCATGGCTTTTCAGGTaaa                >  1:791900/1‑150 (MQ=255)                                                                                                                                    aGCGATAGTTTTTACTTCTCACTAACTGATTTTTCACAGTTTTAACCGTTCATAAATTACCCTGACACAATCATCTGCATTAAAGTAGATGCCAGTTTCTTTGGTCTGATAAATAACGGTTATCGGTGGCGTCATGGCTTTTCAGGTaaa                <  1:502802/150‑1 (MQ=255)                                                                                                                                        aTAGTTTTTACTTCTCACTAACTGATTTTTCACAGTTTTAACCGTTCATAAATTACCCTGACACAATCATCTGCATTAAAGTAGATGCCAGTTTCTTTGGTCTGATAAATAACGGTTATCGGTGGCGTCATGGCTTTTCAGGTAAAAATtc           >  1:347703/1‑151 (MQ=255)                                                                                                                                        aTAGTTTTTACTTCTCACTAACTGATTTTTCACAGTTTTAACCGTTCATAAATTACCCTGACACAATCATCTGCATTAAAGTAGATGCCAGTTTCTTTGGTCTGATAAATAACGGTTATCGGTGGCGTCATGGCTTTTCAGGTAAAAATtc           >  1:223979/1‑151 (MQ=255)                                                                                                                                              tttACTTCTCACTAACTGATTTTTCACAGTTTTAACCGTTCATAAATTACCCTGACACAATCATCTGCATTAAAGTAGATGCCAGTTTCTTTGGTCTGATAAATAACGGTTATCGGTGGCGTCATGGCTTTTCAGGTAAAAATTCATCaa      <  2:346638/150‑1 (MQ=255)                                                                                                                                                 aCTTCTCACTAACTGATTTTTCACAGTTTTAACCGTTCATAAATTACCCTGACACAATCATCTGCATTAAAGTAGATGCCAGTTTCTTTGGTCTGATAAATAACGGTTATCGGTGGCGTCATGGCTTTTCAGGTAAAAATTCATCAAATTc  <  1:870211/151‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                   CAGCCAGACTCTGCATAGTGATGAAAATCACGTCGCCAGTGCGATAATTTTGGGGCAAGCGAATTAACAAATTGATTCAAAGACTCCATAACCTTTCCCGTCATCAGTAAAAAGTGTGACCCGGTTCACGTAGCGATAGTTTTTACTTATCACTAACTGATTTTTCACAGTTTTAACCGTTCATAAATTACCCTGACACAATCATCTGCATTAAAGTAGATGCCAGTTTCTTTGGTCTGATAAATAACGGTTATCGGTGGCGTCATGGCTTTTCAGGTAAAAATTCATCAAATTC  >  NC_000913/1404477‑1404771

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑