Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,476,087	A→C	E315A (GAG→GCG)	ydbD →	PF10971 family putative periplasmic methylglyoxal resistance protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,476,087	0	A	C	100.0%	194.8 / NA	53	E315A (GAG→GCG)	ydbD	PF10971 family putative periplasmic methylglyoxal resistance protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (30/23);  total (30/23)

ACATTGCGACATTTGATCTTGCCGTAATGCGAACTCTCCCAAGGCTTAAAGGAATTTCAGCTAACCTACGCAAACAACTTATTAATAGCAATGATGAACAAACGATTGAAAGTATGGCGAGATATATGCCTGACAATGAGATTCTGGAGTTGACCGATCAGCAATTGGGTTACCAGCCTGTTGTTCTGGGGTTGCTCGACCGTGAACCGCTCTCTGTCGAAATAATGACGCGAATGAGTCGTCTACCTGATGGTGTCGGTCCGTTGAATCTTGCGCTACGTGAAAATCTC  >  NC_000913/1475940‑1476229                                                                                                                                                    |                                                                                                                                               aCATTGCGACATTTGATCTTGCCGTAATGCGAACTCTCCCAAGGCTTAAAGGAATTTCAGCTAACCTACGCAAACAACTTATTAATAGCAATGATGAACAAACGATTGAAAGTATGGCGAGATATATGCCTGACAATGAGATTCTGGCGtt                                                                                                                                             <  1:182505/151‑1 (MQ=255)   aTTGCGACATTTGATCTTGCCGTAATGCGAACTCTCCCAAGGCTTAAAGGAATTTCAGCTAACCTACGCAAACAACTTATTAATAGCAATGATGAACAAACGATTGAAAGTATGGCGAGATATATGCCTGACAATGAGATTCTGGCGTTg                                                                                                                                            >  1:135637/1‑150 (MQ=255)   aTTGCGACATTTGATCTTGCCGTAATGCGAACTCTCCCAAGGCTTAAAGGAATTTCAGCTAACCTACGCAAACAACTTATTAATAGCAATGATGAACAAACGATTGAAAGTATGGCGAGATATATGCCTGACAATGAGATTCTGGCGTTGa                                                                                                                                           <  2:522559/151‑1 (MQ=255)          cATTTGATCTTGCCGTAATGCGAACTCTCCCAAGGCTTAAAGGAATTTCAGCTAACCTACGCAAACAACTTATTAATAGCAATGATGAACAAACGATTGAAAGTATGGCGAGATATATGCCTGACAATGAGATTCTGGCGTTGACCGATc                                                                                                                                     >  2:9873/1‑150 (MQ=255)          cATTTGATCTTGCCGTAATGCGAACTCTCCCAAGGCTTAAAGGAATTTCAGCTAACCTACGCAAACAACTTATTAATAGCAATGATGAACAAACGATTGAAAGTATGGCGAGATATATGCCTGACAATGAGATTCTGGCGTTGACCGATCa                                                                                                                                    <  1:893334/151‑1 (MQ=255)          cATTTGATCTTGCCGTAATGCGAACTCTCCCAAGGCTTAAAGGAATTTCAGCTAACCTACGCAAACAACTTATTAATAGCAATGATGAACAAACGATTGAAAGTATGGCGAGATATATGCCTGACAATGAGATTCTGGCGTTGACCGATCa                                                                                                                                    <  2:195778/151‑1 (MQ=255)               gATCTTGCCGTAATGCGAACTCTCCCAAGGCTTAAAGGAATTTCAGCTAACCTACGCAAACAACTTATTAATAGCAATGATGAACAAACGATTGAAAGTATGGCGAGATATATGCCTGACAATGAGATTCTGGCGTTGACCGATCAGCaa                                                                                                                                <  2:216513/150‑1 (MQ=255)                    tGCCGTAATGCGAACTCTCCCAAGGCTTAAAGGAATTTCAGCTAACCTACGCAAACAACTTATTAATAGCAATGATGAACAAACGATTGAAAGTATGGCGAGATATATGCCTGACAATGAGATTCTGGCGTTGACCGATCAGCAAt                                                                                                                               <  2:248454/146‑1 (MQ=255)                    tGCCGTAATGCGAACTCTCCCAAGGCTTAAAGGAATTTCAGCTAACCTACGCAAACAACTTATTAATAGCAATGATGAACAAACGATTGAAAGTATGGCGAGATATATGCCTGACAATGAGATTCTGGCGTTGACCGATCAGCAATTg                                                                                                                             <  2:113977/148‑1 (MQ=255)                     gCCGTAATGCGAACTCTCCCAAGGCTTAAAGGAATTTCAGCTAACCTACGCAAACAACTTATTAATAGCAATGATGAACAAACGATTGAAAGTATGGCGAGATATATGCCTGACAATGAGATTCTGGCGTTGACCGATCAGCAATTGGGtt                                                                                                                         >  2:575544/1‑151 (MQ=255)                     gCCGTAATGCGAACTCTCCCAAGGCTTAAAGGAATTTCAGCTAACCTACGCAAACAACTTATTAATAGCAATGATGAACAAACGATTGAAAGTATGGCGAGATATATGCCTGACAATGAGATTCTGGCGTTGACCGATCAGCAATTGGGtt                                                                                                                         >  2:203782/1‑151 (MQ=255)                          aaTGCGAACTCTCCCAAGGCTTAAAGGAATTTCAGCTAACCTACGCAAACAACTTATTAATAGCAATGATGAACAAACGATTGAAAGTATGGCGAGATATATGCCTGACAATGAGATTCTGGCGTTGACCGATCAGCAATTGGGTTAcc                                                                                                                      >  2:298645/1‑149 (MQ=255)                            tGCGAACTCTCCCAAGGCTTAAAGGAATTTCAGCTAACCTACGCAAACAACTTATTAATAGCAATGATGAACAAACGATTGAAAGTATGGCGAGATATATGCCTGACAATGAGATTCTGGCGTTGACCGATCAGCAATTGGGTTACCAGcc                                                                                                                  >  2:904079/1‑151 (MQ=255)                               gAACTCTCCCAAGGCTTAAAGGAATTTCAGCTAACCTACGCAAACAACTTATTAATAGCAATGATGAACAAACGATTGAAAGTATGGCGAGATATATGCCTGACAATGAGATTCTGGCGTTGACCGATCAGCAATTGGGTTACCAGCCtgt                                                                                                               >  1:923343/1‑151 (MQ=255)                                  ctctCCCAAGGCTTAAAGGAATTTCAGCTAACCTACGCAAACAACTTATTAATAGCAATGATGAACAAACGATTGAAAGTATGGCGAGATATATGCCTGACAATGAGATTCTGGCGTTGACCGATCAGCAATTGGGTTACCAGCCtgttgt                                                                                                            >  2:653696/1‑151 (MQ=255)                                    ctcCCAAGGCTTAAAGGAATTTCAGCTAACCTACGCAAACAACTTATTAATAGCAATGATGAACAAACGATTGAAAGTATGGCGAGATATATGCCTGACAATGAGATTCTGGCGTTGACCGATCAGCAATTGGGTTACCAGCCtgttgt                                                                                                            <  2:337354/149‑1 (MQ=255)                                      cccAAGGCTTAAAGGAATTTCAGCTAACCTACGCAAACAACTTATTAATAGCAATGATGAACAAACGATTGAAAGTATGGCGAGATATATGCCTGACAATGAGATTCTGGCGTTGACCGATCAGCAATTGGGTTACCAGCCTgttgtt                                                                                                           <  2:443862/148‑1 (MQ=255)                                      cccAAGGCTTAAAGGAATTTCAGCTAACCTACGCAAACAACTTATTAATAGCAATGATGAACAAACGATTGAAAGTATGGCGAGATATATGCCTGACAATGAGATTCTGGCGTTGACCGATCAGCAATTGGGTTACCAGCCTGTTGTTCTg                                                                                                        <  2:407672/151‑1 (MQ=255)                                        cAAGGCTTAAAGGAATTTCAGCTAACCTACGCAAACAACTTATTAATAGCAATGATGAACAAACGATTGAAAGTATGGCGAGATATATGCCTGACAATGAGATTCTGGCGTTGACCGATCAGCAATTGGGTTACCAGCCTGTTGTTCTggg                                                                                                      >  1:94216/1‑151 (MQ=255)                                         aaGGCTTAAAGGAATTTCAGCTAACCTACGCAAACAACTTATTAATAGCAATGATGAACAAACGATTGAAAGTATGGCGAGATATATGCCTGACAATGAGATTCTGGCGTTGACCGATCAGCAATTGGGTTACCAGCCTGTTGTTCTgggg                                                                                                     >  1:777777/1‑151 (MQ=255)                                          aGGCTTAAAGGAATTTCAGCTAACCTACGCAAACAACTTATTAATAGCAATGATGAACAAACGATTGAAAGTATGGCGAGATATATGCCTGACAATGAGATTCTGGCGTTGACCGATCAGCAATTGGGTTACCAGCCTGTTGTTCTGGGGt                                                                                                    >  1:49711/1‑151 (MQ=255)                                           ggCTTAAAGGAATTTCAGCAAACCTACGCCAACAACTTATTAATAGCAATGATGAACAAACGATTGAAAGTATGGCGAGATATATGCCTGACAATGAGATTCTGGCGTTGACCGATCAGCAATTGGGTTACCAGCCTGTTGTTCTgggg                                                                                                     >  1:103727/1‑149 (MQ=255)                                             cTTAAAGGAATTTCAGCTAACCTACGCAAACAACTTATTAATAGCAATGATGAACAAACGATTGAAAGTATGGCGAGATATATGCCTGACAATGAGATTCTGGCGTTGACCGATCAGCAATTGGGTTACCAGCCTGTTGTTCTGGGGTTGc                                                                                                 >  2:299759/1‑151 (MQ=255)                                                aaaGGAATTTCAGCTAACCTACGCAAACAACTTATTAATAGCAATGATGAACAAACGATTGAAAGTATGGCGAGATATATGCCTGACAATGAGATTCTGGCGTTGACCGATCAGCAATTGGGTTACCAGCCTGTTGTTCTGGGGTTGCTCg                                                                                              >  1:866738/1‑151 (MQ=255)                                                aaaGGAATTTCAGCTAACCTACGCAAACAACTTATTAATAGCAATGATGAACAAACGATTGAAAGAATGGCGAGATATATGCCTGACAATGAGATTCTGGCGTTGACCGATCAGCAATTGGGTTACCAGCCTGTTGTTCTGGGGTTGCTCg                                                                                              >  1:637651/1‑151 (MQ=255)                                                      aTTTCAGCTAACCTACGCAAACAACTTATTAATAGCAATGATGAACAAACGATTGAAAGTATGGCGAGATATATGCCTGACAATGAGATTCTGGCGTTGACCGATCAGCAATTGGGTTACCAGCCTGTTGTTCTGGGGTTGCTCGACCGTg                                                                                        >  1:146307/1‑151 (MQ=255)                                                              tAACCTACGCAAACAACTTATTAATAGCAATGATGAACAAACGATTGAAAGTATGGCGAGATATATGCCTGACAATGAGATTCTGGCGTTGACCGATCAGCAATTGGGTTACCAGCCTGTTGTTCTGGGGTTGCTCGACCGTGAACCGctc                                                                                >  2:737344/1‑151 (MQ=255)                                                                 ccTACGCAAACAACTTATTAATAGCAATGATGAACAAACGATTGAAAGTATGGCGAGATATATGCCTGACAATGAGATTCTGGCGTTGACCGATCAGCAATTGGGTTACCAGCCTGTTGTTCTGGGGTTGCTCGACCGTGAACCGctctct                                                                             <  1:573670/151‑1 (MQ=255)                                                                   tACGCAAACAACTTATTAATAGCAATGATGAACAAACGATTGAAAGTATGGCGAGATATATGCCTGACAATGAGATTCTGGCGTTGACCGATCAGCAATTGGGTTACCAGCCTGTTGTTCTGGGGTTGCTCGACCGTGAACCGCTCTCTGt                                                                           >  1:125053/1‑151 (MQ=255)                                                                    aCGCAAACAACTTATTAATAGCAATGATGAACAAACGATTGAAAGTATGGCGAGATATATGCCTGACAATGAGATTCTGGCGTTGACCGATCAGCAATTGGGTTACCAGCCTGTTGTTCTGGGGTTGCTCGACCGTGAACCGCTCTCTGt                                                                           <  1:254473/150‑1 (MQ=255)                                                                    aCGCAAACAACTTATTAATAGCAATGATGAACAAACGATTGAAAGTATGGCGAGATATATGCCTGACAATGAGATTCTGGCGTTGACCGATCAGCAATTGGGTTACCAGCCTGTTGTTCTGGGGTTGCTCGACCGTGAACCGCTCTCTGTc                                                                          >  2:814512/1‑151 (MQ=255)                                                                       cAAACAACTTATTAATAGCAATGATGAACAAACGATTGAAAGTATGGCGAGATATATGCCTGACAATGAGATTCTGGCGTTGACCGATCAGCAATTGGGTTACCAGCCTGTTGTTCTGGGGTTGCTCGACCGTGAACCGCTCTCTGTCGa                                                                        >  2:709596/1‑150 (MQ=255)                                                                        aaaCAACTTATTAATAGCAATGATGAACAAACGATTGAAAGTATGGCGAGATATATGCCTGACAATGAGATTCTGGCGTTGACCGATCAGCAATTGGGTTACCAGCCTGTTGTTCTGGGGTTGCTCGACCGTGAACCGCTCTCTGTCGaaa                                                                      <  1:823586/151‑1 (MQ=255)                                                                        aaaCAACTTATTAATAGCAATGATGAACAAACGATTGAAAGTATGGCGAGATATATGCCTGACAATGAGATTCTGGCGTTGACCGATCAGCAATTGGGTTACCAGCCTGTTGTTCTGGGGTTGCTCGACCGTGAACCGCTCTCTGTCGaa                                                                       >  2:410866/1‑150 (MQ=255)                                                                          acaacTTATTAATAGCAATGATGAACAAACGATTGAAAGTATGGCGAGATATATGCCTGACAATGAGATTCTGGCGTTGACCGATCAGCAATTGGGTTACCAGCCTGTTGTTCTGGGGTTGCTCGACCGTGAACCGCTCTCTGTCGAaat                                                                     >  2:261911/1‑150 (MQ=255)                                                                                tattaATAGCAATGATGAACAAACGATTGAAAGTATGGCGAGATATATGCCTGACAATGAGATTCTGGCGTTGACCGATCAGCAATTGGGTTACCAGCCTGTTGTTCTGGGGTTGCTCGACCGTGAACCGCTCTCTGTCGAAATAATGAc                                                               <  2:240429/150‑1 (MQ=255)                                                                                tattaATAGCAATGATGAACAAACGATTGAAAGTATGGCGAGATATATGCCTGACAATGAGATTCTGGCGTTGACCGATCAGCAATTGGGTTACCAGCCTGTTGTTCTGGGGTTGCTCGACCGTGAACCGCTCTCTGTCGAAATAATGAc                                                               <  1:629539/150‑1 (MQ=255)                                                                                  ttaATAGCAATGATGAACAAACGATTGAAAGTATGGCGAGATATATGCCTGACAATGAGATTCTGGCGTTGACCGATCAGCAATTGGGTTACCAGCCTGTTGTTCTGGGGTTGCTCGACCGTGAACCGCTCTCTGTCGAAATAATGAcgcg                                                            >  2:348544/1‑151 (MQ=255)                                                                                       aGCAATGATGAACAAACGATTGAAAGTATGGCGAGATATATGCCTGACAATGAGATTCTGGCGTTGACCGATCAGCAATTGGGTTACCAGCCTGTTGTTCTGGGGTTGCTCGACCGTGAACCGCTCTCTGTCGAAATAATGACGCGAATg                                                        >  1:269902/1‑150 (MQ=255)                                                                                            tgatgaACAAACGATTGAAAGTATGGCGAGATATATGCCTGACAATGAGATTCTGGCGTTGACCGATCAGCAATTGGGTTACCAGCCTGTTGTTCTGGGGTTGCTCGACCGTGAACCGCTCTCTGTCGAAATAATGACGCGAATGAgtcgt                                                  >  1:862572/1‑151 (MQ=255)                                                                                             gatgaACAAACGATTGAAAGTATGGCGAGATATATGCCTGACAATGAGATTCTGGCGTTGACCGATCAGCAATTGGGTTACCAGCCTGTTGTTCTGGGGTTGCTCGACCGTGAACCGCTCTCTGTCGAAATAATGACGCGAATGAgtcgt                                                  <  1:510308/150‑1 (MQ=255)                                                                                              atgaACAAACGATTGAAAGTATGGCGAGATATATGCCTGACAATGAGATTCTGGCGTTGACCGATCAGCAATTGGGTTACCAGCCTGTTGTTCTGGGGTTGCTCGACCGTGAACCGCTCTCTGTCGAAATAATGACGCGAATGAgtcgtc                                                 <  1:522251/150‑1 (MQ=255)                                                                                                         aTTGAAAGTATGGCGAGATATATGCCTGACAATGAGATTCTGGCGTTGACCGATCAGCAATTGGGTTACCAGCCTGTTGTTCTGGGGTTGCTCGACCGTGAACCGCTCTCTGTCGAAATAATGACGCGAATGAGTCGTCTACCTGATGgtg                                     <  1:611875/151‑1 (MQ=255)                                                                                                          ttGAAAGTATGGCGAGATATATGCCTGACAATGAGATTCTGGCGTTGACCGATCAGCAATTGGGTTACCAGCCTGTTGTTCTGGGGTTGCTCGACCGTGAACCGCTCTCTGTCGAAATAATGACGCGAATGAGTCGTCTACCTGATGgtgt                                    >  2:924566/1‑151 (MQ=255)                                                                                                                   tGGCGAGATATATGCCTGACAATGAGATTCTGGCGTTGACCGATCAGCAATTGGGTTACCAGCCTGTTGTTCTGGGGTTGCTCGACCGTGAACCGCTCTCTGTCGAAATAATGACGCGAATGAGTCGTCTACCTGATGGTGTCGGTCCGtt                           >  2:328697/1‑151 (MQ=255)                                                                                                                    ggCGAGATATATGCCTGACAATGAGATTCTGGCGTTGACCGATCAGCAATTGGGTTACCAGCCTGTTGTTCTGGGGTTGCTCGACCGTGAACCGCTCTCTGTCGAAATAATGACGCGAATGAGTCGTCTACCTGATGGTGTCGGTCCGtt                           <  2:205579/150‑1 (MQ=255)                                                                                                                    ggCGAGATATATGCCTGACAATGAGATTCTGGCGTTGACCGATCAGCAATTGGGTTACCAGCCTGTTGTTCTGGGGTTGCTCGACCGTGAACCGCTCTCTGTCGAAATAATGACGCGAATGAGTCGTCTACCTGATGGTGTCGGTCCGTTg                          <  1:7131/151‑1 (MQ=255)                                                                                                                       gagaTATATGCCTGACAATGAGATTCTGGCGTTGACCGATCAGCAATTGGGTTACCAGCCTGTTGTTCTGGGGTTGCTCGACCGTGAACCGCTCTCTGTCGAAATAATGACGCGAATGAGTCGTCTACCTGATGGTGTCGGTCCGTTGAAt                       >  2:390828/1‑151 (MQ=255)                                                                                                                              atGCCTGACAATGAGATTCTGGCGTTGACCGATCAGCAATTGGGTTACCAGCCTGTTGTTCTGGGGTTGCTCGACCGTGAACCGCTCTCTGTCGAAATAATGACGCGAATGAGTCGTCTACCTGATGGTGTCGGTCCGTTGAATCTTgcgc                >  1:161047/1‑151 (MQ=255)                                                                                                                              atGCCTGACAATGAGATTCTGGCGTTGACCGATCAGCAATTGGGTTACCAGCCTGTTGTTCTGGGGTTGCTCGACCGTGAACCGCTCTCTGTCGAAATAATGACGCGAATGAGTCGTCTACCTGATGGTGTCGGTCCGTTGAATCTTgcgc                <  1:22817/151‑1 (MQ=255)                                                                                                                                 ccTGACAATGAGATTCTGTCGTTGACCGATCAGCAATTGGGTTACCAGCCTGTTGTTCTGGGGTTGCTCGACCGTGAACCGCTCTCTGTCGAAATAATGACGCGAATGAGTCGTCTACCTGATGGTATCGGTCCGTTGAATCTTGCGCTAc             >  1:378613/1‑151 (MQ=255)                                                                                                                                    gACAATGAGATTCTGGCGTTGACCGATCAGCAATTGGGTTACCAGCCTGTTGTTCTGGGGTTGCTCGACCGTGAACCGCTCTCTGTCGAAATAATGACGCGAATGAGTCGTCTACCTGATGGTGTCGGTCCGTTGAATCTTGCGCTACGt           <  1:404327/150‑1 (MQ=255)                                                                                                                                            gaTTCTGGCGTTGACCGATCAGCAATTGGGTTACCAGCCTGTTGTTCTGGGGTTGCTCGACCGTGAACCGCTCTCTGTCGAAATAATGACGCGAATGAGTCGTCTACCTGATGGTGTCGGTCCGTTGAATCTTGCGCTACGTGAAAAtctc  <  1:436632/151‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                               ACATTGCGACATTTGATCTTGCCGTAATGCGAACTCTCCCAAGGCTTAAAGGAATTTCAGCTAACCTACGCAAACAACTTATTAATAGCAATGATGAACAAACGATTGAAAGTATGGCGAGATATATGCCTGACAATGAGATTCTGGAGTTGACCGATCAGCAATTGGGTTACCAGCCTGTTGTTCTGGGGTTGCTCGACCGTGAACCGCTCTCTGTCGAAATAATGACGCGAATGAGTCGTCTACCTGATGGTGTCGGTCCGTTGAATCTTGCGCTACGTGAAAATCTC  >  NC_000913/1475940‑1476229

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑