Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,486,129	T→G	G1023G (GGT→GGG)	hrpA →	putative ATP‑dependent helicase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,486,129	0	T	G	100.0%	156.5 / NA	44	G1023G (GGT→GGG)	hrpA	putative ATP‑dependent helicase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (26/18);  total (26/18)

TGGTGGATGACAAAAACAAGAAGCTAAAAGAAGGGCGCTCGCTACAAGATCTGAAAGATGCGCTGAAAGGCAAAGTGCAGGAAACGCTATCTGCGGTGGCGGATGACGGTATCGAGCAGAGCGGCTTACATATCTGGAGTTTTGGTCAGCTGCCGGAAAGCTACGAACAGAAGCGTGGCAACTACAAAGTGAAGGCGTGGCCGGCGCTGGTGGATGAGCGCGACAGTGTGGCGATCAAACTGTTTGATAACCCGCTGGAGCAAAAGCAGGCAATGTGGAACGGTCTTCGC  >  NC_000913/1485984‑1486273                                                                                                                                                  |                                                                                                                                                 tggtggATGACAAAAACAAGAAGCTAAAAGAAGGGCGCTCGCTACAAGATCTGAAAGATGCGCTGAAAGGCAAAGTGCAGGAAACGCTATCTGCGGTGGCGGATGACGGTATCGAGCAGAGCGGCTTACATATCTGGAGTTTTGGGCAGCt                                                                                                                                             >  2:754157/1‑151 (MQ=255)         gACAAAAACAAGAAGCTAAAAGAAGGGCGCTCGCTACAAGATCTGAAAGATGCGCTGAAAGGCAAAGTGCAGGAAACGCTATCTGCGGTGGCGGATGACGGTATCGAGCAGAGCGGCTTACATATCTGGAGTTTTGGGCAGCTGCCGGaaa                                                                                                                                     <  2:84634/151‑1 (MQ=255)                aCAAGAAGCTAAAAGAAGGGCGCTCGCTACAAGATCTGAAAGATGCGCTGAAAGGCAAAGTGCAGGAAACGCTATCTGCGGTGGCGGATGACGGTATCGAGCAGAGCGGCTTACATATCTGGAGTTTTGGGCAGCTGCCGGAAAGCTACg                                                                                                                               >  1:414988/1‑150 (MQ=255)                    gaagCTAAAAGAAGGGCGCTCGCTACAAGATCTGAAAGATGCGCTGAAAGGCAAAGTGCAGGAAACGCTATCTGCGGTGGCGGATGACGGTATCGAGCAGAGCGGCTTACATATCTGGAGTTTTGGGCAGCTGCCGGAAAGCTACGAAc                                                                                                                            >  1:407197/1‑149 (MQ=255)                        cTAAAAGAAGGGCGCTCGCTACAAGATCTGAAAGATGCGCTGAAAGGCAAAGTGCAGGAAACGCTATCTGCGGTGGCGGATGACGGTATCGAGCAGAGCGGCTTACATATCTGGAGTTTTGGGCAGCTGCCGGAAAGCTACGAACAGAAGc                                                                                                                      >  2:33981/1‑151 (MQ=255)                                 gggCGCTCGCTACAAGATCTGAAAGATGCGCTGAAAGGCAAAGTGCAGGAAACGCTATCTGCGGTGGCGGATGACGGTATCGAGCAGAGCGGCTTACATATCTGGAGTTTTGGGCAGCTGCCGGAAAGCTACGAACAGAAGCGTGGCAACt                                                                                                             >  1:845242/1‑151 (MQ=255)                                            aCAAGATCTGAAAGATGCGCTGAAAGGCAAAGTGCAGGAAACGCTATCTGCGGTGGCGGATGACGGTATCGAGCAGAGCGGCTTACATATCTGGAGTTTTGGGCAGCTGCCGGAAAGCTACGAACAGAAGCGTGGCAACTACAAAGTGa                                                                                                    >  1:881973/1‑149 (MQ=255)                                              aaGATCTGAAAGATGCGCTGAAAGGCAAAGTGCAGGAAACGCTATCTGCGGTGGCGGATGACGGTATCTAGCAGAGCGGCTTACATATCTGGAGTTCTGGGCAGCTGCCGGAAAGCTACGAACAGAAGCGTGGCATCTACAAAGTGAAgg                                                                                                 <  1:669843/150‑1 (MQ=255)                                                   cTGAAAGATGCGCTGAAAGGCAAAGTGCAGGAAACGCTATCTGCGGTGGCGGATGACGGTATCGAGCAGAGCGGCTTACATATCTGGAGTTTTGGGCAGCTGCCGGAAAGCTACGAACAGAAGCGTGGCAACTACAAAGTGAAGGCGTGGc                                                                                           >  1:441409/1‑151 (MQ=255)                                                     gAAAGATGCGCTGAAAGGCAAAGTGCAGGAAACGCTATCTGCGGTGGCGGATGACGGTATCGAGCAGAGCGGCTTACATATCTGGAGTTTTGGGCAGCTGCCGGAAAGCTACGAACAGAAGCGTGGCAACTACAAAGTGAAGGCGTGGcc                                                                                          <  1:825010/150‑1 (MQ=255)                                                     gAAAGATGCGCTGAAAGGCAAAGTGCAGGAAACGCTATCTGCGGTGGCGGATGACGGTATCGAGCAGAGCGGCTTACATATCTGGAGTTTTGGGCAGCTGCCGGAAAGCTACGAACAGAAGCGTGGCAACTACAAAGTGAAGGCGTGGCCg                                                                                         <  2:529384/151‑1 (MQ=255)                                                      aaaGATGCGCTGAAAGGCAAAGTGCAGGAAACGCTATCTGCGGTGGCGGATGACGGTATCGAGCAGAGCGGCTTACATATCTGGAGTTTTGGGCAGCTGCCGGAAAGCTACGAACAGAAGCGTGGCAACTACAAAGTGAAGGCGTGGCCgg                                                                                        >  2:305921/1‑151 (MQ=255)                                                               cTGAAAGGCAAAGTGCAGGAAACGCTATCTGCGGTGGCGGATGACGGTATCGAGCAGAGCGGCTTACATATCTGGAGTTTTGGGCAGCTGCCGGAAAGCTACGAACAGAAGCGTGGCAACTACAAAGTGAAGGCGTGGCCGGCGCtgggg                                                                                >  2:215285/1‑148 (MQ=255)                                                                      gCAAAGTGCAGGAAACGCTATCTGCGGTGGCGGATGACGGTATCGAGCAGAGCGGCTTACATATCTGGAGTTTTGGGCAGCTGCCGGAAAGCTACGAACAGAAGCGTGGCAACTACAAAGTGAAGGCGTGGCCGGCGCTGGTGGATGAgcg                                                                        <  1:30973/151‑1 (MQ=255)                                                                       cAAAGTGCAGGAAACGCTATCTGCGGTGGCGGATGACGGTATCGAGCAGAGCGGCTTACATATCTGGAGTTTTGGGCAGCTGCCGGAAAGCTACGAACAGAAGCGTGGCAACTACAAAGTGAAGGCGTGGCCGGCGCTGGTGGATGAgcgc                                                                       <  2:919969/151‑1 (MQ=255)                                                                                ggAAACGCTATCTGCGGTGGCGGATGACGGTATCGAGCAGAGCGGCTTACATATCTGGAGTTTTGGGCAGCTGCCGGAAAGCTACGAACAGAAGCGTGGCAACTACAAAGTGAAGGCGTGGCCGGCGCTGGTGGATGAGCGCGACAGtgtg                                                              >  1:858976/1‑151 (MQ=255)                                                                                ggAAACGCTATCTGCGGTGGCGGATGACGGTATCGAGCAGAGCGGCTTACATATCTGGAGTTTTGGGCAGCTGCCGGAAAGCTACGAACAGAAGCGTGGCAACTACAAAGTGAAGGCGTGGCCGGCGCTGGTGGATGAGCGCGACAGtgtg                                                              <  2:568888/151‑1 (MQ=255)                                                                                 gAAACGCTATCTGCGGTGGCGGATGACGGTATCGAGCAGAGCGGCTTACATATCTGGAGTTTTGGGCAGCTGCCGGAAAGCTACGAACAGAAGCGTGGCAACTACAAAGTGAAGGCGTGGCCGGCGCTGGTGGATGAGCGCGACAGTGTgg                                                             >  1:674576/1‑151 (MQ=255)                                                                                    aCGCTATCTGCGGTGGCGGATGACGGTATCGAGCAGAGCGGCTTACATATCTGGAGTTTTGGGCAGCTGCCGGAAAGCTACGAACAGAAGCGTGGCAACTACAAAGTGAAGGCGTGGCCGGCGCTGGTGGATGAGCGCGACAGTGTGGCGa                                                          <  1:836655/151‑1 (MQ=255)                                                                                      gCTATCTGCGGTGGCGGATGACGGTATCGAGCAGAGCGGCTTACATATCTGGAGTTTTGGGCAGCTGCCGGAAAGCTACGAACAGAAGCGTGGCAACTACAAAGTGAAGGCGTGGCCGGCGCTGGTGGATGAGCGCGACAGTGTGGCGATc                                                        >  2:415163/1‑151 (MQ=255)                                                                                               ggTGGCGGATGACGGTATCGAGCAGCGCGGCTTACATATCTGGAGTTTTGGGCAGCTGCCGGAAAGCTACGAACAGAAGCGCGGCAACTACAACGTGAAGGCGTGGCCGGCGCTGGTGGATGAGCGCGACAGTGTGGCGATCAAACTGttt                                               >  1:784925/1‑151 (MQ=255)                                                                                               ggTGGCGGAGGACGGTATCGAGCAGAGCGGCTTACATATCTGTGGATTTGGGCAGCTTCCGGAAAGCTACGAACAGAATGGTGGCAACGACAACGTTAAGGCGGGGCCGGCGCGGGGGTATGAGCGCGACAGTGTGGCGAtaagac                                                    >  1:806443/1‑141 (MQ=255)                                                                                                   gCGGATGACGGTATCGAGCAGAGCGGCTTACATATCTGGAGTTTTGGGCAGCTGCCGGAAAGCTACGAACAGAAGCGTGGCAACTACAAAGTGAAGGCGTGGCCGGCGCTGGTGGATGAGCGCGACAGTGTGGCGATCAAACTGTTTGATa                                           <  1:422402/151‑1 (MQ=255)                                                                                                    cGGATGACGGTATCGAGCAGAGCGGCTTACATATCTGGAGTTTTGGGCAGCTGCCGGAAAGCTACGAACAGAAGCGTGGCAACTACAAAGTGAAGGCGTGGCCGGCGCTGGTGGATGAGCGCGACAGTGTGGCGATCAAACTGTTTGATa                                           >  2:84643/1‑150 (MQ=255)                                                                                                      gATGACGGTATCGAGCAGAGCGGCTTACATATCTGGAGTTTTGGGCAGCTGCCGGAAAGCTACGAACAGAAGCGTGGCAACTACAAAGTGAAGGCGTGGCCGGCGCTGGTGGATGAGCGCGACAGTGTGGCGATCAAACTGTTTGATAAcc                                        <  2:599154/151‑1 (MQ=255)                                                                                                      gATGACGGTATCGAGCAGAGCGGCTTACATATCTGGAGTTTTGGGCAGCTGCCCGAAAGCTACGAACAGAAGCGTGGCAACTACAAAGTGAAGGCGTGGCCGGCGCTGGTGGATGAGCGCGACAGTGTGGCGATCAAACTGTTTGATAAcc                                        >  2:96876/1‑151 (MQ=255)                                                                                                       aTGACGGTATCGAGCAGAGCGGCTTACATATCTGGAGTTTTGGGCAGCTGCCGGAAAGCTACGAACAGAAGCGTGGCAACTACAAAGTGAAGGCGTGGCCGGCGCTGGTGGATGAGCGCGACAGTGTGGCGATCAAACTGTTTGATAAccc                                       <  1:374587/151‑1 (MQ=255)                                                                                                       aTGACGGTATCGAGCAGAGCGGCTTACATATCTGGAGTTTTGGGCAGCTGCCGGAAAGCTACGAACAGAAGCGTGGCAACTACAAAGTGAAGGCGTGGCCGGCGCTGGTGGATGAGCGCGACAGTGTGGCGATCAAACTGTTTGATAAccc                                       <  1:488214/151‑1 (MQ=255)                                                                                                             gTATCGAGCAGAGCGGCTTACATATCTGGAGTTTTGGGCAGCTGCCGGAAAGCTACGAACAGAAGCGTGGCAACTACAAAGTGAAGGCGTGGCCGGCGCTGGTGGATGAGCGCGACAGTGTGGCGATCAAACTGTTTGATAACCCGCTgg                                  >  2:709535/1‑150 (MQ=255)                                                                                                              tATCGAGCAGAGCGGCTTACATATCTGGAGTTTTGGGCAGCTGCCGGAAAGCTACGAACAGAAGCGTGGCAACTACAAAGTGAAGGCGTGGCCGGCGCTGGTGGATGAGCGCGACAGTGTGGCGATCAAACTGTTTGATAACCCGCTgg                                  >  2:593648/1‑149 (MQ=255)                                                                                                                tCGAGCAGAGCGGCTTACATATCTGGAGGTTTGGGCAGCTGCCGGAAAGCTACGAACAGAAGCGTGGCAACTACAAAGTGAAGGCGTGGCCGGCGCTGGTGGATGAGCGGGACAGTGTGGCGATCAAACTGTTTGATAACCCGCTGGAGc                               >  2:290517/1‑150 (MQ=255)                                                                                                                    gCAGAGCGGCTTACATATCTGGAGTTTTGGGCAGCTGCCGGAAAGCTACGAACAGAAGCGTGGCAACTACAAAGTGAAGGCGTGGCCGGCGCTGGTGGATGAGCGCGACAGTGTGGCGATCAAACTGTTTGATAACCCGCTGGAGCAAAAg                          <  1:794633/151‑1 (MQ=255)                                                                                                                         gCGGCTTACATATCTGGAGTTTTGGGCAGCTGCCGGAAAGCTACGAACAGAAGCGTGGCAACTACAAAGTGAAGGCGTGGCCGGCGCTGGTGGATGAGCGCGACAGTGTGGCGATCAAACTGTTTGATAACCCGCTGGAGCAAAAGCAGGc                     >  1:603189/1‑151 (MQ=255)                                                                                                                          cGGCTTACATATCTGGAGTTTTGGGCAGCTGCCGGAAAGCTACGAACAGAAGCGTGGCAACTACAAAGTGAAGGCGTGGCCGGCGCTGGTGGATGAGCGCGACAGTGTGGCGATCAAACTGTTTGATAACCCGCTGGAGCAAAAGCAGGCa                    <  2:683879/151‑1 (MQ=255)                                                                                                                             cTTACATATCTGGAGTTTTGGGCAGCTGCCGGAAAGCTACGAACAGAAGCGTGGCAACTACAAAGTGAAGGCGTGGCCGGCGCTGGTGGATGAGCGCGACAGTGTGGCGATCAAACTGTTTGATAACCCGCTGGAGCAAAAGCAGGCAAtg                 >  1:864436/1‑151 (MQ=255)                                                                                                                                aCATATCTGGAGTTTTGGGCAGCTGCCGGAAAGCTACGAACAGAAGCGTGGCAACTACAAAGTGAAGGCGTGGCCGGCGCTGGTGGATGAGCGCGACAGTGTGGCGATCAAACTGTTTGATAACCCGCTGGAGCAAAAGCAGGCAATGTgg              >  2:496845/1‑151 (MQ=255)                                                                                                                                aCATATCTGGAGTTTTGGGCAGCTGCCGGAAAGCTACGAACAGAAGCGTGGCAACTACAAAGTGAAGGCGTGGCCGGCGCTGGTGGATGAGCGCGACAGTGTGGCGATCAAACTGTTTGATAACCCGCTGGAGCAAAAGCAGGCAATGTgg              >  2:418204/1‑151 (MQ=255)                                                                                                                                   tatCTGGAGTTTTGGGCAGCTGCCGGAAAGCTACGAACAGAAGCGTGGCAACTACAAAGTGAAGGCGTGGCCGGCGCTGGTGGATGAGCGCGACAGTGTGGCGATCAAACTGTTTGATAACCCGCTGGAGCAAAAGCAGGCAATGTGGAAc           >  2:514721/1‑151 (MQ=255)                                                                                                                                    atCTGGAGTTTTGGGCAGCTGCCGGAAAGCTACGAACAGAAGCGTGGCAACTACAAAGTGAAGGCGTGGCCGGCGCTGGTGGATGAGCGCGACAGTGTGGCGATCAAACTGTTTGATAACCCGCTGGAGCAAAAGCAGGCAATGTGGAACg          >  2:742174/1‑151 (MQ=255)                                                                                                                                      cTGGAGTTTTGGGCAGCTGCCGGAAAGCTACGAACAGAAGCGTGGCAACTACAAAGTGAAGGCGTGGCCGGCGCTGGTGGATGAGCGCGACAGTGTGGCGATCAAACTGTTTGATAACCCGCTGGAGCAAAAGCAGGCAATGTGGAACGGt        >  2:173426/1‑151 (MQ=255)                                                                                                                                        ggAGTTTTGGGCAGCTGCCGGAAAGCTACGAACAGAAGCGTGGCAACTACAAAGTGAAGGCGTGGCCGGCGCTGGTGGATGAGCGCGACAGTGTGGCGATCAAACTGTTTGATAACCCGCTGGAGCAAAAGCAGGCAATGTGGAACg          <  1:4304/147‑1 (MQ=255)                                                                                                                                         gAGTTTTGGGCAGCTGCCGGAAAGCTACGAACAGAAGCGTGGCAACTACAAAGTGAAGGCGTGGCCGGCGCTGGTGGATGAGCGCGACAGTGTGGCGATCAAACTGTTTGATAACCCGCTGGAGCAAAAGCAGGCAATGTGGAACGGTCtt     <  1:609202/151‑1 (MQ=255)                                                                                                                                           gTTTTGGGCAGCTGCCGGAAAGCTACGAACAGAAGCGTGGCAACTACAAAGTGAAGGCGTGGCCGGCGCTGGTGGATGAGCGCGACAGTGTGGCGATCAAACTGTTTGATAACCCGCTGGAGCAAAAGCAGGCAATGTGGAACGGTCTTCg   <  2:485107/151‑1 (MQ=255)                                                                                                                                             tttGGGCAGCTGCCGGAAAGCTACGAACAGAAGCGTGGCAACTACAAAGTGAAGGCGTGGCCGGCGCTGGTGGATGAGCGCGACAGTGTGGCGATCAAACTGTTTGATAACCCGCTGGAGCAAAAGCAGGCAATGTGGAACGGTCTTCGc  <  2:80461/150‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                                 TGGTGGATGACAAAAACAAGAAGCTAAAAGAAGGGCGCTCGCTACAAGATCTGAAAGATGCGCTGAAAGGCAAAGTGCAGGAAACGCTATCTGCGGTGGCGGATGACGGTATCGAGCAGAGCGGCTTACATATCTGGAGTTTTGGTCAGCTGCCGGAAAGCTACGAACAGAAGCGTGGCAACTACAAAGTGAAGGCGTGGCCGGCGCTGGTGGATGAGCGCGACAGTGTGGCGATCAAACTGTTTGATAACCCGCTGGAGCAAAAGCAGGCAATGTGGAACGGTCTTCGC  >  NC_000913/1485984‑1486273

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑