Predicted mutation | ||||||
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evidence | seq id | position | mutation | annotation | gene | description |
RA | NC_000913 | 1,498,172 | A→C | E439D (GAA→GAC) | opgD → | OPG biosynthetic periplasmic protein |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 1,498,172 | 0 | A | C | 98.2% | 195.4 / ‑6.8 | 57 | E439D (GAA→GAC) | opgD | OPG biosynthetic periplasmic protein |
Reads supporting (aligned to +/- strand): ref base A (0/0); major base C (36/20); minor base T (1/0); total (37/20) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 |
TGTCTGCTTCTGGCAGCCAGAAAAAGCTGTAAAAGCAGGTGATGAGTTTGCATTCCAGTATCGTCTGTACTGGAGTGCGCAACCGCCTGTTCATTGCCCATTAGCGCGCGTTATGGCGACGCGTACCGGCATGGGCGGTTTCTCGGAAGGTTGGGCGCCAGGTGAACACTATCCCGAAAAATGGGCGCGTCGTTTTGCCGTCGATTTCGTTGGTGGTGATCTGAAAGCTGCCGCGCCAAAAGGCATTGAGCCGGTGATTACGCTTTCCAGTGGGGAAGCGAAGCAAATCGAAATT > NC_000913/1498025‑1498319 | tGTCTGCTTCTGGCAGCCAGAAAAAGCTGTAAAAGCAGGTGATGAGTTTGCATTCCAGTATCGTCTGTACTGGAGTGCGCAACCGCCTGTTCATTGCCCATTAGCGCGCGTTATGGCGACGCGTACCGGCATGGGCGGTTTCTCGGACgg > 1:726919/1‑150 (MQ=255) ttCTGGCAGCCAGAAAAAGCTGTAAAAGCAGGTGTTGATTTTGCATTCCAGTATCGTTTGTCCTGGAGTGCGCAACCGCCTGTTCATTGCCCATTAGCGCGCGTTATGGCGACGCGTACCGGCATGGGCGGTTTCTCGGACGGtt < 2:408804/145‑1 (MQ=255) ttCTGGCAGCCAGAAAAAGCTGTAAAAGCAGGTGATGAGTTTGCATTCCAGTATCGTCTGTACTGGAGTGCGCAACCGCCTGTTCATTGCCCATTAGCGCGCGTTATGGCGACGCGTACCGGCATGGGCGGTTTCTCGGACGGTTGGgc < 1:709491/149‑1 (MQ=255) tCTGGCAGCCAGAAAAAGCTGTAAAAGCGGGTGATGAGTTTGCATCCCAGTATCGTCTGTACTGGGGTGCGCAACCGCCTGTTCATTGCCCATTAGCGCGCGTTATGGCGACGCGTACCGGCATGGGCGGTTTCTCGGACGGTTGGgcgc < 1:140035/150‑1 (MQ=255) tCTGGCAGCCAGAAAAAGCTGTAAAAGCAGGTGATGAGTTTGCATTCCAGTATCGTCTGTACTGGAGTGCGCAACCGCCTGTTCATTGCCCATTAGCGCGCGTTATGGCGACGCGTACCGGCATGGGCGGTTTCTCGGACGGTTGGGCGcc > 2:408629/1‑151 (MQ=255) cTGGCAGCCAGAAAAAGCTGTAAAAGCAGGTGATGAGTTTGCATTCCAGTATCGTCTGTACTGGAGTGCGCAACCGCCTGTTCATTGCCCATTAGCGCGCGTTATGGCGACGCGTACCGGCATGGGCGGTTTCTCGGACGGTTGGGCGcc > 2:459842/1‑150 (MQ=255) tGGCAGCCAGAAAAAGCTGTAAAAGCAGGTGATGAGTTTGCATTCCAGTATCGTCTGTACTGGAGTGCGCAACCGCCTGTTCATTGCCCATTAGCGCGCGTTATGGCGACGCGTACCGGCATGGGCGGTTTCTCGGACGGTTGGGCGcc > 1:662110/1‑149 (MQ=255) ggCAGCCAGAAAAAGCTATAAAAGCGGGTGATGAGTTTGCATTCCAGTATCTTCTGTACTGGAGTGCGCAACCGCCTGTTCATTGCCCATTAGCGCGCGTTATGGCGACGCGTACCGGCATGGGCGGTTTCTCGGACGGTTGGGCGcc < 2:442376/148‑1 (MQ=255) gCCAGAAAAAGCTGTAAAAGCAGGTGATGAGTTTGCATTCCAGTATCGTCTGTACTGGAGTGCGCAACCGCCTGTTCATTGCCCATTAGCGCGCGTTATGGCGACGCGTACCGGCATGGGCGGTTTCTCGGACGGTTGGGCGCCAGGTGa > 1:714913/1‑150 (MQ=255) aGAAAAAGCTGTAAAAGCAGGTGATGATTTTGCATTCCAGTATCGTCTGTGCTGGAGTGCGCAACCGCCTGTTCATTGCCCATTAGCGCGCGTTATGGCGACGCGTACCGGCATGGGCGGTTTCTCGGACGGTTGGGCGCCAGGTGAaca < 1:689026/150‑1 (MQ=255) gAAAAAGCTGTAAAAGCAGGTGATGAGTTTGCATTCCAGTATCGTCTGTACTGGAGTGCGCAACCGCCTGTTCATTGCCCATTAGCGCGCGTTATGGCGACGCGTACCGGCATGGGCGGTTTCTCGGACGGTTGGGCGCCAGGTGAACACt > 1:135920/1‑151 (MQ=255) aaaaGCTGTAAAAGCAGGTGATGAGTTTGCATTCCAGTATCGTCTGTACTGGAGTGCGCAACCGCCTGTTCATTGCCCATTAGCGCGCGTTATGGCGACGCGTACCGGCATGGGCGGATTCTCGGACGGTTGGGCGCCAGGTGAACACTAt > 1:38771/1‑151 (MQ=255) aaGCTGTAAAAGCAGGTGATGAGTTTGCATTCCAGTATCGTCTGTACTGGAGTGCGCAACCGCCTGTTCATTGCCCATTAGCGCGCGTTATGGCGACGCGTACCGGCATGGGCGGTTTCTCGGACGGTTGGGCGCCAGGTGAACACTATcc > 1:1874/1‑151 (MQ=255) tGTAAAAGCAGGTGATGAGTTTGCATTCCAGTATCGTCTGTACTGGAGTGCGCAACCGCCTGTTCATTGCCCATTAGCGCGCGTTATGGCGACGCGTACCGGCATGGGCGGTTTCTCGGACGGTTGGGCGCCAGGTGAACACTATCCCGa > 1:140887/1‑150 (MQ=255) gTAAAAGCAGGTGATGAGTTTGCATTCCAGTATCGTCTGTACTGGAGTGCGCAACCGCCTGTTCATTGCCCATTAGCGCGCGTTATGGCGACGCGTACCGGCATGGGCGGTTTCTCGGACGGTTGGGCGGCAGGTGAACACTATCCCGa > 1:610669/1‑149 (MQ=255) gCAGGTGATGAGTTTGCATTCCAGTATCGTCTGTACTGGAGTGCGCAACCGCCTGTTCATTGCCCATTAGCGCGCGTTATGGCGACGCGTACCGGCATGGGCGGTTTCTCGGACGGTTGGGCGCCAGGTGAACACTATCCCGAAAAATgg < 2:909344/150‑1 (MQ=255) ggTGATGAGTTTGCATTCCAGTATCGTCTGTACTGGAGTGCGCAACCGCCTGTTCATTGCCCATTAGCGCGCGTTATGGCGACGCGTACCGGCATGGGCGGTTTCTCGGACGGTTGGGCGCCAGGTGAACACTATCCCGAAAAATGGgcg < 1:142392/150‑1 (MQ=255) gatgaGTTTGCATTCCAGTATCGTCTGTACTGGAGTGCGCAACCGCCTGTTCATTGCCCATTAGCGCGCGTTATGGCGACGCGTACCGGCATGGGCGGTTTCTCGGACGGTTGGGCGCCAGGTGAACACTATCCCGAAAAATGGGCGcgtc > 1:887233/1‑151 (MQ=255) tgaGTTTGCATTCCAGTATCGTCTGTACTGGAGTGCGCAACCGCCTGTTCATTGCCCATTAGCGCGCGTTATGGCGACGCGTACCGGCATGGGCGGTTTCTCGGACGGTTGGGCGCCAGGCGAACACTATCCCGAAAAATGGGCGcgtcgt > 2:847966/1‑151 (MQ=255) aGTTTGCATTCCAGTATCGTCTGTACTGGAGTGCGCAACCGCCTGTTCATTGCCCATTAGCGCGCGTTATGGCGACGCGTACCGGCATGGGCGGTTTCTCGGACGGTTGGGCGCCAGGTGAACACTATCCCGAAAAATGGGCGCGTCGttt > 2:828295/1‑151 (MQ=255) gTTTGCATTCCAGTATCGTCTGTACTGGAGTGCGCAACCGCCTGTTCATTGCCCATTAGCGCGCGTTATGGCGACGCGTACCGGCATGGGCGGTTTCTCGGACGGTTGGGCGCCAGGTGAACACTATCCCGAAAAATGGGCGCGTCGtttt > 1:915708/1‑151 (MQ=255) tttGCATTCCAGTATCTTCTGTACTGGAGTGCGCAACCGCCTGTTCATTGCCCATTAGCGCGCGTTATGGCGACGCGTACCGGCATGGGCGGTTTCTCGGACGGTTGGGCGCCAGGTGAACACTATCCCGAAAAATGGGCGCGTCGtttt < 2:847891/150‑1 (MQ=255) cATTCCAGTATCGTCTGTACTGGAGTGCGCAACCGCCTGTTCATTGCCCATTAGCGCGCGTTATGGCGACGCGTACCGGCATGGGCGGTTTCTCGGACGGTTGGGCGCCAGGTGAACACTATCCCGAAAAATGGGCGCGTCGTTTTGCCGt > 2:563262/1‑151 (MQ=255) cATTCCAGTATCGTCTGTACTGGAGTGCGCAACCGCCTGTTCATTGCCCATTAGCGCGCGTTATGGCGACGCGTACCGGCATGGGCGGTTTCTCGGACGGTTGGGCGCCAGGTGAACACTATCCCGAAAAATGGGCGCGTCGTTTTGCCGt < 1:95176/151‑1 (MQ=255) aTTCCAGTATCGTCTGTACTGGAGTGCGCAACCGCCTGTTCATTGCCCATTAGCGCGCGTTATGGCGACGCGTACCGGCATGGGCGGTTTCTCGGACGGTTGGGCGCCAGGTGAACACTATCCCGAAAAATGGGCGCGTCGTTTTGCCGTc > 1:567287/1‑151 (MQ=255) ccAGTATCGTCTGTACTGGAGTGCGCAACCGCCTGTTCATTGCCCATTAGCGCGCGTTATGGCGACGCGTACCGGCATGGGCGGTTTCTCGGACGGTTGGGCGCCAGGTGAACACTATCCCGAAAAATGGGCGCGTCGTTTTGCCGTCGa < 2:918158/150‑1 (MQ=255) aGTATCGTCTGTACTGGAGTGCGCAACCGCCTGTTCATTGCCCATTAGCGCGCGTTATGGCGACGCGTACCGGCATGGGCGGTTTCTCGGACGGTTGGGCGCCAGGTGAACACTATCCCGAAAAATGGGCGCGTCGTTTTGCCGTCGAttt > 2:856979/1‑151 (MQ=255) gTCTGTACTGGAGTGCGCAACCGCCTGTTCATTGCCCATTAGCGCGCGTTATGGCGACGCGTACCGGCATGGGCGGTTTCTCGGACGGTTGGGCGCCAGGTGAACACTATCCCGAAAAATGGGCGCGTCGTTTTGCCGTCGATTTCGTtgg > 2:253103/1‑151 (MQ=255) cTGGAGTGCGCAACCGCCTGTTCATTGCCCATTAGCGCGCGTTATGGCGACGCGTACCGGCATGGGCGGTTTCTCGGACGGTTGGGCGCCAGGTGAACACTATCCCGAAAAATGGGCGCGTCGTTTTGCCGTCGATTTCGTTGGTGGTGAt > 2:80030/1‑151 (MQ=255) ggAGTGCGCAACCGCCTGTTCATTGCCCATTAGCGCGCGTTATGGCGACGCGTACCGGCATGGGCGGTTTCTCGGACGGTTGGGCGCCAGGTGAACACTATCCCGAAAAATGGGCGCGTCGTTTTGCCGTCGATTTCGTTGGTGGTGATCt > 2:27948/1‑151 (MQ=255) gAGTGCGCAACCGCCTGTTCATTGCCCATTAGCGCGCGTTATGGCGACGCGTACCGGCATGGGCGGTTTCTCGGACGGTTGGGCGCCAGGTGAACACTATCCCGAAAAATGGGCGCGTCGTTTTGCCGTCGATTTCGTTGGTGGTGATCTg < 2:115393/151‑1 (MQ=255) aaCCGCCTGTTCATTGCCCATTAGCGCGCGTTATGGCGACGCGTACCGGCATGGGCGGTTTCTCGGACGGTTGGGCGCCAGGTGAACACTATCCCGAAAAATGGGCGCGTCGTTTTGCCGTCGATTTCGTTGGTGGTGATCTGAAAGCTGc < 2:501883/151‑1 (MQ=255) aaCCGCCTGTTCATTGCCCATTAGCGCGCGTTATGGCGACGCGTACCGGCATGGGCGGTTTCTCGGACGGTTGGGCGCCAGGTGAACACTATCCCGAAAAATGGGCGCGTCGTTTTGCCGTCGATTTCGTTGGTGGTGATCTGAAAGCTGc > 2:422203/1‑151 (MQ=255) cGCCTGTTCATTGCCCATTAGCGCGCGTTATGGCGACGCGTACCGGCATGGGCGGTTTCTCGGACGGTTGGGCGCCAGGTGAACACTATCCCGAAAAATGGGCGCGTCGTTTTGCCGTCGATTTCGTTGGTGGTGATCTGAAAGCTGCcgc > 1:574076/1‑151 (MQ=255) ccTGTTCATTGCCCATTAGCGCGCGTTATGGCGACGCGTACCGGCATGGGCGGTTTCTCGGACGGTTGGGCGCCAGGTGAACACTATCCCGAAAAATGGGCGCGTCGTTTTGCCGTCGATTTCGTTGGTGGTGATCTGAAAGCTGCCgcgc > 2:147032/1‑151 (MQ=255) gTTCATTGCCCATTAGCGCGCGTTATGGCGACGCGTACCGGCATGGGCGGTTTCTCGGACGGTTGGGCGCCAGGTGAACACTATCCCGAAAAATGGGCGCGTCGTTTTGCCGTCGATTTCGTTGGTGGTGATCTGAAAGCTGCCGCGCCa > 1:353866/1‑150 (MQ=255) aTTGCCCATTAGCGCGCGTTATGGCGACGCGTACCGGCATGGGCGGTTTCTCGGACGGTTGGGCGCCAGGTGAACACTATCCCGAAAAATGGGCGCGTCGTTTTGCCGTCGATTTCGTTGGTGGTGATCTGAAAGCTGCCGCGCCAAAAgg > 1:334132/1‑151 (MQ=255) gCCCATTAGCGCGCGTTATGGCGACGCGTACCGGCATGGGCGGTTTCTCGGACGGTTGGGCGCCAGGTGAACACTATCCCGAAAAATGGGCGCGTCGTTTTGCCGTCGATTTCGTTGGTGGTGATCTGAAAGCTGCCGCGCCAAAAGGCAt > 1:288841/1‑151 (MQ=255) aTTAGCGCGCGTTATGGCGACGCGTACCGGCATGGGCGGTTTCTCGGACGGTTGGGCGCCAGGTGAACACTATCCCGAAAAATGGGCGCGTCGTTTTGCCGTCGATTTCGTTGGTGGTGATCTGAAAGCTGCCGCGCCAAAAGGCATTg > 1:535815/1‑149 (MQ=255) aGCGCGCGTTATGGCGACGCGTACCGGCATGGGCGGTTTCTCGGACGGTTGGGCGCCAGGTGAACACTATCCCGAAAAATGGGCGCGTCGTTTTGCCGTCGATTTCGTTGGTGGTGATCTGAAAGCTGCCGCGCCAAAAGGCATTGAGCCg > 2:898140/1‑151 (MQ=255) cgcgcgTTATGGCGACGCGTACCGGCATGGGCGGTTTCTCGGACGGTTGGGCGCCAGGTGAACACTATCCCGAAAAATGGGCGCGTCGTTTTGCCGTCGATTTCGTTGGTGGTGATCTGAAAGCTGCCGCGCCAAAAGGCATTGAGCCgg > 2:761274/1‑150 (MQ=255) cgTTATGGCGACGCGTACCGGCATGGGCGGTTTCTCGGACGGTTGGGCGCCAGGTGAACACTATCCCGAAAAATGGGCGCGTCGTTTTGCCGTCGATTTCGTTGGTGGTGATCTGAAAGCTGCCGCGCCAAAAGGCATTGAGCCGGTGAtt > 1:704722/1‑151 (MQ=255) gTTATGGCGACGCGTACCGGCATGGGCGGTTTCTCGGATGGTTGGGCGACAGGTGAACACTATCCCGAAAAATGGGCGCGTCGTTTTGCCGTCGCTTTTGTTGGTGGTGATCTGAAAACTGCCGCGCCAAAAGGCATTGAGCCGGTGAt > 2:642618/1‑149 (MQ=255) ggCGACGCGTACCGGCATGGGCGGTTTCTCGGACGGTTGGGCGCCAGGTGAACACTATCCCGAAAAATGGGCGCGTCGTTTTGCCGTCGATTTCGTTGGTGGTGATCTGAAAGCTGCCGCGCCAAAAGGCATTGAGCCGGTGATTACg < 1:168229/148‑1 (MQ=255) ggCGACGCGTACCGGCATGGGCGGTTTCTCGGACGGTTGGGCGCCAGGTGAACACTATCCCGAAAAATGGGCGCGTCGTTTTGCCGTCGATTTCGTTGGTGGTGATCTGAAAGCTGCCGCGCCAAAAGGCATTGAGCCGGTGATTACGCtt < 1:879980/151‑1 (MQ=255) cgTACCGGCATGGGCGGATTCTCGGACGGGTGGGCGCCAGGTGAACACTATCCCGAAAAATGCGCGCGGCGTTTTGCCGGCGATTTCGTTGGGGGCGATCTGAAAGCTGCCGCGCCAAAAGGCATTGAGCCGGTGATTACGCTTTCCAgcg > 1:35870/1‑149 (MQ=255) gTACCGGCATGGGCGGTTTCTCGGACGGTTGGGCGCCAGGTGAACACTATCCCGAAAAATGGGCGCGTCGTTTTGCCGTCGATTTCGTTGGTGGTGATCTGAAAGCTGCCGCGCCAAAAGGCATTGAGCCGGTGATTACGCTTTCCAGTgg > 2:271924/1‑151 (MQ=255) aCCGGCATGGGCGGTTTCTCGGACGGTTGGGCGCCAGGTGAACACTATCCCGAAAAATGGGCGCGTCGTTTTGCCGTCGATTTCGTTGGTGGTGATCTGAAAGCTGCCGCGCCAAAAGGCATTGAGCCGGTGATTACGCTTTCCAGTgggg > 1:887570/1‑151 (MQ=255) gCATGGGCGGTTTCTCGGACGGTTGGGCGCCAGGTGAACACTATCCCGAAAAATGGGCGCGTCGTTTTGCCGTCGATTTCGTTGGTGGTGATCTGAAAGCTGCCGCGCCAAAAGGCATTGAGCCGGTGATTACGCTTTCCAGTGGGgaagc < 2:166510/151‑1 (MQ=255) gggCGGTTTCTCGGACGGTTGGGCGCCAGGTGAACACTATCCCGAAAAATGGGCGCGTCGTTTTGCCGTCGATTTCGTTGGTGGTGATCTGAAAGCTGCCGCGCCAAAAGGCATTGAGCCGGTGATTACGCTTTCCAGTGGGgaagcgaa < 1:296823/150‑1 (MQ=255) ggCGGTTTCTCGGACGGTTGGGCGCCAGGTGAACACTATCCCGAAAAATGGGCGCGTCGTTTTGCCGTCGATTTCGTTGGTGGTGATCTGAAAGCTGCCGCGCCAAAAGGCATTGAGCCGGTGATTACGCTTTCCAGTGGGgaagcgaagc < 1:141817/151‑1 (MQ=255) gTTTCTCGGACGGTTGGGCGCCAGGTGAACACTATCCCGAAAAATGGGCGCGTCGTTTTGCCGTCGATTTCGTTGGTGGTGATCTGAAAGCTGCCGCGCCAAAAGGCATTGAGCCGGTGATTACGCTTTCCAGTGGGGAAGCGAAGCAAAt < 2:261853/151‑1 (MQ=255) ttCTCGGACGGTTGGGCGCCAGGTGAACACTATCCCGAAAAATGGGCGCGTCGTTTTGCCGTCGATTTCGTTGGTGGTGATCTGAAAGCTGCCGCGCCAAAAGGCATTGAGCCGGTGATTACGCTTTCCAGTGGGGAAGCGAAGCAAATCg > 1:902495/1‑151 (MQ=255) cGGACGGTTGGGCGCCAGGTGAACACTATCCCGAAAAATGGGCGCGTCGTTTTGCCGTCGATTTCGTTGGTGGTGATCTGAAAGCTGCCGCGCCAAAAGGCATTGAGCCGGTGATTACGCTTTCCAGTGGGGAAGCGAAGCAAATCGaa < 2:662130/149‑1 (MQ=255) cGGACGGTTGGGCGCCAGGTGAACACTATCCCGAAAAATGGGCGCGTCGTTTTGCCGTCGATTTCGTTGGTGGTGATCTGAAAGCTGCCGCGCCAAAAGGCATTGAGCCGGTGATTACGCTTTCCAGTGGGGAAGCGAAGCAAATCGAAAt > 1:73551/1‑151 (MQ=255) ggACGGTTGGGCGCCAGGTGAACACTATCCCGAAAAATGGGCGCGTCGTTTTGCCGTCGATTTCGTTGGTGGTGATCTGAAAGCTGCCGCGCCAAAAGGCATTGAGCCGGTGATTACGCTTTCCAGTGGGGAAGCGAAGCAAATCGAAAtt < 1:895053/151‑1 (MQ=255) ggACGGTTGGGCGCCAGGTGAACACTATCCCGAAAAATGGGCGCGTCGTTTTGCCGTCGATTTCGTTGGTGGTGATCTGAAAGCTGCCGCGCCAAAAGGCATTGAGCCGGTGATTACGCTTTCCAGTGGGGAAGCGAAGCAAATCGAAAt > 2:151483/1‑150 (MQ=255) | TGTCTGCTTCTGGCAGCCAGAAAAAGCTGTAAAAGCAGGTGATGAGTTTGCATTCCAGTATCGTCTGTACTGGAGTGCGCAACCGCCTGTTCATTGCCCATTAGCGCGCGTTATGGCGACGCGTACCGGCATGGGCGGTTTCTCGGAAGGTTGGGCGCCAGGTGAACACTATCCCGAAAAATGGGCGCGTCGTTTTGCCGTCGATTTCGTTGGTGGTGATCTGAAAGCTGCCGCGCCAAAAGGCATTGAGCCGGTGATTACGCTTTCCAGTGGGGAAGCGAAGCAAATCGAAATT > NC_000913/1498025‑1498319 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |