Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	99,341	G→T	V313V (GTG→GTT)	ftsW →	putative lipid II flippase; integral membrane protein; FtsZ ring stabilizer

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	99,341	0	G	T	100.0%	233.7 / NA	61	V313V (GTG→GTT)	ftsW	putative lipid II flippase; integral membrane protein; FtsZ ring stabilizer
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (26/35);  total (26/35)

TAACGCAATCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTGCCGGAAGCGCACACTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTGCTGGCACTTTTAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTAAAGCATTAGAAATTGACCACCGTTTTTCCGGTTTTCTCGCCTGTTCTATTGGCATCTGGTTTAGCTTCCAGGCGCTGGTTAACGTAGGCGCG  >  NC_000913/99193‑99488                                                                                                                                                     |                                                                                                                                                    tAACGCAATCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTGCCGGAAGCGCACACTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTTCt                                                                                                                                                   >  2:92166/1‑151 (MQ=255) tAACGCAATCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTGCCGGAAGCGCACACTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTTCt                                                                                                                                                   >  1:299978/1‑151 (MQ=255)  aaCGCAATCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTGCCGGAAGCGCACACTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTTCt                                                                                                                                                   <  2:656772/150‑1 (MQ=255)       aaTCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTGCCGGAAGCGCACACTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTTCTGGCACt                                                                                                                                             >  2:498650/1‑151 (MQ=255)       aaTCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTGCCGGAAGCGCACACTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTTCTGGCACt                                                                                                                                             >  2:891672/1‑151 (MQ=255)             tgctGGCGTTTGGCCGCGGCGAACTTTGGGGGCAAGGTTTAGATAACTCGGTACAAAAACTGGAGTATCTGCCGGAAGCGCACACTGACTTTATTTTCGCCATTATCGGCGAAGAAATGGGGTATGTCGGTGTGGATCTGGCACTTTTaa                                                                                                                                        >  1:825856/4‑150 (MQ=255)              gATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTGCCGGAAGCGCACACTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTTCTGGCACTTTTAAt                                                                                                                                       <  1:898054/150‑1 (MQ=255)              gATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTGCCGGAAGCGCACACTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTTCTGGCACTTTTAATg                                                                                                                                      <  1:498287/151‑1 (MQ=255)               aTGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTGCCGGAAGCGCACACTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTTCTGGCACTTTTAATgg                                                                                                                                     <  1:523846/151‑1 (MQ=255)                  gCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTGCCGGAAGCGCACACTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTTCTGGCACTTTTAATGGTAt                                                                                                                                  <  2:293434/151‑1 (MQ=255)                     tttGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTGCCGGAAGCGCACACTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTTCTGGCACTTTTAATGGTAttct                                                                                                                               >  2:925778/1‑151 (MQ=255)                      ttGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTGCCGGAAGCGCACACTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTTCTGGCACTTTTAATGGTAttct                                                                                                                               <  1:103480/150‑1 (MQ=255)                        ggTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTGCCGGAAGCGCACACTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTTCTGGCACTTTTAATGGTAttcttc                                                                                                                             <  2:834850/150‑1 (MQ=255)                            gcggcgAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTGCCGGAAGCGCACACTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTTCTGGCACTTTTAATGGTATTCTTCGTCGc                                                                                                                        <  1:392415/151‑1 (MQ=255)                             aggcgAACTTTGGGGGCAAGGTTTAGGAAACTCGGTACAAAACCTGGAGTATCTGCCGGAAGCGCACACTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTTCTGGCACTTTTAATGGTATTCTTCGTCGCt                                                                                                                       >  1:669828/2‑151 (MQ=255)                               gcgAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTGCCGGAAGCGCACACTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTTCTGGCACTTTTAATGGTATTCTTCGTCGCttt                                                                                                                     <  2:173281/151‑1 (MQ=255)                                 gAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTGCCGGAAGCGCACACTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTTCTGGCACTTTTAATGGTATTCTTCGTCGCTTTTc                                                                                                                   <  2:864414/151‑1 (MQ=255)                                   aCTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTGCCGGAAGCGCACACTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTTCTGGCACTTTTAATGGTATTCTTCGTCGCTTTTcgc                                                                                                                 >  1:580123/1‑151 (MQ=255)                                      ttGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTGCCGGAAGCGCACACTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTTCTGGCACTTTTAATGGTATTCTTCGTCGCTTTTcgcg                                                                                                                <  1:651356/149‑1 (MQ=255)                                        gggggCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTGCCGGAAGCGCACACTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTTCTGGCACTTTTAATGGTATTCTTCGTCGCTTTTCGCGCGAt                                                                                                            <  1:876249/151‑1 (MQ=255)                                            gCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTGCCGGAAGCGCACACTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTTCTGGCACTTTTAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCg                                                                                                        >  2:478616/1‑151 (MQ=255)                                                  tttAGGTAACTCGGTACAAAAACTGGAGTATCTGCCGGAAGCGCACACTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTTCTGGCACTTTTAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGc                                                                                                  >  2:134993/1‑151 (MQ=255)                                                       gTAACTCGGTACAAAAACTGGAGTATCTGCCGGAAGCGCACACTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTTCTGGCACTTTTAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTaa                                                                                             <  1:654449/151‑1 (MQ=255)                                                         aaCTCGGTACAAAAACTGGAGTATCTGCCGGAAGCGCACACTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTTCTGGCACTTTTAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTaaa                                                                                            <  2:807709/150‑1 (MQ=255)                                                          aCTCGGTACAAAAACTGGAGTATCTGCCGGAAGCGCACACTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTTCTGGCACTTTTAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTAAAGc                                                                                          <  1:42822/151‑1 (MQ=255)                                                          aCTCGGTACAAAAACTGGAGTATCTGCCGGAAGCGCACACTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTTCTGGCACTTTTAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTAAAGc                                                                                          >  2:503688/1‑151 (MQ=255)                                                            tCGGTACAAAAACTGGAGTATCTGCCGGAAGCGCACACTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTTCTGGCACTTTTAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTAAAGCAt                                                                                        >  2:181375/1‑151 (MQ=255)                                                             cGGTACAAAAACTGGAGTATCTGCCGGAAGCGCACACTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTTCTGGCACTTTTAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTAAAGCAtt                                                                                       >  2:898559/1‑151 (MQ=255)                                                                 aCAAAAACTGGAGTATCTGCCGGAAGCGCACACTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTTCTGGCACTTTTAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTAAAGCATTAGaa                                                                                   <  2:622073/151‑1 (MQ=255)                                                                 aCAAAAACTGGAGTATCTGCCGGAAGCGCACACTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTTCTGGCACTTTTAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTAAAGCATTAGa                                                                                    <  2:619564/150‑1 (MQ=255)                                                                  cAAAAACTGGAGTATCTGCCGGAAGCGCACACTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTTCTGGCACTTTTAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTAAAGCATTAGaa                                                                                   <  2:731743/150‑1 (MQ=255)                                                                         tGGAGTATCTGCCGGAAGCGCACACTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTTCTGGCACTTTTAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTAAAGCATTAGAAATTGacc                                                                            >  2:727487/1‑150 (MQ=255)                                                                          ggAGTATCTGCCGGAAGCGCACACTGACTTTATTTTCGCCATTATCGGCGAAGAGCTGGGGTATGTCGGTGTGGTTCTGGCACTTTTAATGGTAGTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTAAAGCATTAGAAATTGacca                                                                           <  1:272484/150‑1 (MQ=255)                                                                          ggAGTATCTGCCGGAAGCGCACACTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTTCTGGCACTTTTAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTAAAGCATTAGAAATTGaccac                                                                          <  1:595228/151‑1 (MQ=255)                                                                          ggAGTATCTGCCGGAAGCGCACACTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTTCTGGCACTTTTAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTAAAGCATTAGAAATTGacca                                                                           <  2:257004/150‑1 (MQ=255)                                                                          ggAGTATCTGCCGGAAGCGCACACTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTTCTGGCACTTTTAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTAAAGCATTAGAAATTGacc                                                                            <  1:27907/149‑1 (MQ=255)                                                                               aTCTGCCGGAAGCGCACACTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTTCTGGCACTTTTAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTAAAGCATTAGAAATTGACCACCGttt                                                                     >  1:792559/1‑151 (MQ=255)                                                                                   gCCGGAAGCGCACACTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTTCTGGCACTTTTAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTAAAGCATTAGAAATTGACCACCGTTTTTcc                                                                 <  2:823128/151‑1 (MQ=255)                                                                                           cgcACACTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTTCTGGCACTTTTAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTAAAGCATTAGAAATTGACCACCGTTTTTCCGGTTTtct                                                         <  2:247421/151‑1 (MQ=255)                                                                                            gctcacTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTTCTGGCACTTTTAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTAAAGCATTAGAAATTGACCACCGTTTTTCCGGTTTtctc                                                        <  1:41810/148‑1 (MQ=255)                                                                                             cacaCTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTTCTGGCACTTTTAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTAAAGCATTAGAAATTGACCACCGTTTTTCCGGTTTTCTCg                                                       >  2:187896/1‑151 (MQ=255)                                                                                             cacaCTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTTCTGGCACTTTTAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTAAAGCATTAGAAATTGACCACCGTTTTTCCGGTTTTCTCg                                                       <  1:896645/151‑1 (MQ=255)                                                                                               cacTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTTCTGGCACTTTTAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTAAAGCATTAGAAATTGACCACCGTTTTTCCGGTTTTCTCGcc                                                     >  2:166561/1‑151 (MQ=255)                                                                                                 cTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTTCTGGCACTTTTAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTAAAGCATTAGAAATTGACCACCGTTTTTCCGGTTTTCTCGCCTg                                                   >  1:424759/1‑151 (MQ=255)                                                                                                    aCTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTTCTGGCACTTTTAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTAAAGCATTAGAAATTGACCACCGTTTTTCCGGTTTTCTCGCCTGTTc                                                <  2:833514/151‑1 (MQ=255)                                                                                                       ttATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTTCTGGCACTTTTAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTAAAGCATTAGAAATTGACCACCGTTTTTCCGGTTTTCTCGCCTGTTCTa                                              <  2:199076/150‑1 (MQ=255)                                                                                                           tttCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTTCTGGCACTTTTAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTAAAGCATTAGAAATTGACCACCGTTTTTCCGGTTTTCTCGCCTGTTCTATTGGc                                         >  1:473144/1‑151 (MQ=255)                                                                                                              cGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTTCTGGCACTTTTAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTAAAGCATTAGAAATTGACCACCGTTTTTCCGGTTTTCTCGCCTGTTCTATTGGCAt                                       <  2:700576/150‑1 (MQ=255)                                                                                                               gCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTTCTGGCACTTTTAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTAAAGCATTAGAAATTGACCACCGTTTTTCCGGTTTTCTCGCCTGTTCTATTGGCATCt                                     >  2:181913/1‑151 (MQ=255)                                                                                                               gCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTTCTGGCACTTTTAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTAAAGCATTAGAAATTGACCACCGTTTTTCCGGTTTTCTCGCCTGTTCTATTGGCATCt                                     <  1:255178/151‑1 (MQ=255)                                                                                                                     aTCGGCGAAGAACTGGGGTATGTCGGTGTGGTTCTGGCACTTTTAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTAAAGCATTAGAAATTGACCACCGTTTTTCCGGTTTTCTCGCCTGTTCTATTGGCATCTGGTTTa                               <  2:119448/151‑1 (MQ=255)                                                                                                                       cGGCGAAGAACTGGGGTATGTCGGTGTGGTTCTGGCACTTTTAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTAAAGCATTAGAAATTGACCACCGTTTTTCCGGTTTTCTCGCCTGTTCTATTGGCATCTGGTTTAGc                             <  1:349391/151‑1 (MQ=255)                                                                                                                       cGGCGAAGAACTGGGGTATGTCGGTGTGGTTCTGGCACTTTTAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTAAAGCATTAGAAATTGACCACCGTTTTTCCGGTTTTCTCGCCTGTTCTATTGGCATCTGGTTTAGc                             >  1:360223/1‑151 (MQ=255)                                                                                                                           gaagaaCTGGGGTATGTCGGTGTGGTTCTGGCACTTTTAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTAAAGCATTAGAAATTGACCACCGTTTTTCCGGTTTTCTCGCCTGTTCTATTGGCATCTGGTTTAGCTTcc                         >  2:365401/1‑151 (MQ=255)                                                                                                                              gaaCTGGGGTATGTCGGTGTGGTTCTGGCACTTTTAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTAAAGCATTAGAAATTGACCACCGTTTTTCCGGTTTTCTCGCCTGTTCTATTGGCATCTGGTTTAGCTTCCAgg                      <  2:241895/151‑1 (MQ=255)                                                                                                                                 cTGGGGTATGTCGGTGTGGTTCTGGCACTTTTAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTAAAGCATTAGAAATTGACCACCGTTTTTCCGGTTTTCTCGCCTGTTCTATTGGCATCTGGTTTAGCTTCCAGgcgc                   >  2:52844/1‑151 (MQ=255)                                                                                                                                   ggggTATGTCGGTGTGGTTCTGGCACTTTTAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTAAAGCATTAGAAATTGACCACCGTTTTTCCGGTTTTCTCGCCTGTTCTATTGGCATCTGGTTTAGCTTCCAGgcgc                   <  2:552337/149‑1 (MQ=255)                                                                                                                                   ggggTATGTCGGTGTGGTTCTGGCACTTTTAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTAAAGCATTAGAAATTGACCACCGTTTTTCCGGTTTTCTCGCCTGTTCTATTGGCATCTGGTTTAGCTTCCAGGCGCTg                 >  2:669598/1‑151 (MQ=255)                                                                                                                                     ggTATGTCGGTGTGGTTCTGGCACTTTTAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTAAAGCATTAGAAATTGACCACCGTTTTTCCGGTTTTCTCGCCTGTTCTATTGGCATCTGGTTTAGCTTCCAGGCGCTGGt               <  1:488853/151‑1 (MQ=255)                                                                                                                                             ggTGTGGTTCTGGCACTTTTAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTAAAGCATTAGAAATTGACCACCGTTTTTCCGGTTTTCTCGCCTGTTCTATTGGCATCTGGTTTAGCTTCCAGGCGCTGGTTAACGt         >  1:62573/1‑149 (MQ=255)                                                                                                                                               tgtgGTTCTGGCACTTTTAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTAAAGCATTAGAAATTGACCACCGTTTTTCCGGTTTTCTCGCCTGTTCTATTGGCATCTGGTTTAGCTTCCAGGCGCTGGTTAACGTAgg      >  1:233386/1‑150 (MQ=255)                                                                                                                                                gtggttCTGGCACTTTTAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTAAAGCATTAGAAATTGACCACCGTTTTTCCGGTTTTCTCGCCTGTTCTATTGGCATCTGGTTTAGCTTCCAGGCGCTGGTTAACGTAGgc     <  1:925757/150‑1 (MQ=255)                                                                                                                                                  ggttCTGGCACTTTTAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTAAAGCATTAGAAATTGACCACCGTTTTTCCGGTTTTCTCGCCTGTTCTATTGGCATCTGGTTTAGCTTCCAGGCGCTGGTTAACGTAGGcgcg  >  1:269657/1‑151 (MQ=255)                                                                                                                                                     |                                                                                                                                                    TAACGCAATCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTGCCGGAAGCGCACACTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTGCTGGCACTTTTAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTAAAGCATTAGAAATTGACCACCGTTTTTCCGGTTTTCTCGCCTGTTCTATTGGCATCTGGTTTAGCTTCCAGGCGCTGGTTAACGTAGGCGCG  >  NC_000913/99193‑99488

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑