| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,638,290 | T→G | intergenic (‑181/+165) | ynfN ← / ← cspI | Qin prophage; cold shock‑induced protein/Qin prophage; cold shock protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,638,290 | 0 | T | G | 98.3% | 202.7 / ‑3.1 | 59 | intergenic (‑181/+165) | ynfN/cspI | Qin prophage; cold shock‑induced protein/Qin prophage; cold shock protein |
| Reads supporting (aligned to +/- strand): ref base T (0/1); new base G (27/30); total (27/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.71e-01 | |||||||||||
GCTGGTTCAGAGTTAAAAAAACTATGATTTTTTTCATGATGTTACCGTAGTATGTGAGTATCCATCGAATAGACACCAAGCAAAAAAGCTCCCGAAGGAGCCTTCATTTTCACTTTTTTAAATCCAACGACAGACGGCTGGCATTTAAGTATTGTGAAATATTATCAAATGTAATCATCATTGATTTACAAAAGATACATTTTGCCCCGAAAGGATTCATGTCAGAAACATCAAAAGATGATGTTCTATACTGGGAACCATGACAACACGGGCATCTAAAGTGAATATGGT > NC_000913/1638147‑1638437 | gctgGTTCAGAGTTAAAAAAACTATGATTTTTTTCATGATGTTACCGTAGTATGTGAGTATCCATCGAATAGACACCAAGCAAAAAAGCTCCCGAAGGAGCCTTCATTTTCACTTTTTTAAATCCAACGACAGACGGCTGGCAGTTAAGt < 1:375314/150‑1 (MQ=255) ggTTCAGAGTTAAAAAAACTATGATTTTTTTCATGATGTTACCGTAGTATGTGAGTATCCATCGAATAGACACCAAGCAAAAAAGCTCCCGAAGGAGCCTTCATTTTCACTTTTTTAAATCCAACGACAGACGGCTGGCAGTTAAGTAtt < 1:444775/150‑1 (MQ=255) ggTTCAGAGTTAAAAAAACTATGATTTTTTTCATGATGTTACCGTAGTATGTGAGTATCCATCGAATAGACACCAAGCAAAAAAGCTCCCGAAGGAGCCTTCATTTTCACTTTTTTAAATCCAACGACAGACGGCTGGCAGTTAAGTATtg < 2:934958/151‑1 (MQ=255) ggTTCAGAGTTAAAAAAACTATGATTTTTTTCATGATGTTACCGTAGTATGTGAGTATCCATCGAATAGACACCAAGCAAAAAAGCTCCCGAAGGAGCCTTCATTTTCACTTTTTTAAATCCAACGACAGACGGCTGGCAGTTAAGTATtg < 1:689638/151‑1 (MQ=255) tCAGAGTTAAAAAAACTATGATTTTTTTCATGATGTTACCGTAGTATGTGAGTATCCATCGAATAGACACCAAGCAAAAAAGCTCCCGAAGGAGCCTTCATTTTCACTTTTTTAAATCCAACGACAGACGGCTGGCAGTTAAGTATtgtg < 2:362729/150‑1 (MQ=255) tCAGAGTTAAAAAAACTATGATTTTTTTCATGATGTTACCGTAGTATGTGAGTATCCATCGAATAGACACCAAGCAAAAAAGCTCCCGAAGGAGCCTTCATTTTCACTTTTTTAAATCCAACGACAGACGGCTGGCAGTTAAGTATtgtg < 2:654068/150‑1 (MQ=255) agagTTAAAAAAACTATGATTTTTTTCATGATGTTACCGTAGTATGTGAGTATCCATCGAATAGACACCAAGCAAAAAAGCTCCCGAAGGAGCCTTCATTTTCACTTTTTTAAATCCAACGACAGACGGCTGGCAGTTAAGTATTGTGaaa < 2:332547/151‑1 (MQ=255) agagTTAAAAAAACTATGATTTTTTTCATGATGTTACCGTAGTATGTGAGTATCCATCGAATAGACACCAAGCAAAAAAGCTCCCGAAGGAGCCTTCATTTTCACTTTTTTAAATCCAACGACAGACGGCTGGCAGTTAAGTATTGTGaa > 1:794762/1‑150 (MQ=255) agTTAAAAAAACTATGATTTTTTTCATGATGTTACCGTAGTATGTGAGTATCCATCGAATAGACACCAAGCAAAAAAGCTCCCGAAGGAGCCTTCATTTTCACTTTTTTAAATCCAACGACAGACGGCTGGCAGTTAAGTATTGTGAAata < 2:903578/151‑1 (MQ=255) ttAAAAAAACTATGATTTTTTTCATGATGTTACCGTAGTATGTGAGTATCCATCGAATAGACACCAAGCAAAAAAGCTCCCGAAGGAGCCTTCATTTTCACTTTTTTAAATCCAACGACAGACGGCTGGCAGTTAAGTATTGTGAAAtatt > 2:4004/1‑151 (MQ=255) ttAAAAAAACTATGATTTTTTTCATGATGTTACCGTAGTATGTGAGTATCCATCGAATAGACACCAAGCAAAAAAGCTCCCGAAGGAGCCTTCATTTTCACTTTTTTAAATCCAACGACAGACGGCTGGCAGTTAAGTATTGTGAAAtatt > 2:282864/1‑151 (MQ=255) tAAAAAAACTATGATTTTTTTCATGATGTTACCGTAGTATGTGAGTATCCATCGAATAGACACCAAGCAAAAAAGCTCCCGAAGGAGCCTTCATTTTCACTTTTTTAAATCCAACGACAGACGGCTGGCAGTTAAGTATTGTGAAAtatt < 1:148341/150‑1 (MQ=255) aaaaaCTATGATTTTTTTCATGATGTTACCGTAGTATGTGAGTATCCATCGAATAGACACCAAGCAAAAAAGCTCCCGAAGGAGCCTTCATTTTCACTTTTTTAAATCCAACGACAGACGGCTGGCAGTTAAGTATTGTGAAATATTATc > 1:598935/1‑150 (MQ=255) aaaaaCTATGATTTTTTTCATGATGTTACCGTAGTATGTGAGTATCCATCGAATAGACACCAAGCAAAAAAGCTCCCGAAGGAGCCTTCATTTTCACTTTTTTAAATCCAACGACAGACGGCTGGCAGTTAAGTATTGTGAAATATTATCa < 2:599103/151‑1 (MQ=255) aCTATGATTTTTTTCATGATGTTACCGTAGTATGTGAGTATCCATCGAATAGACACCAAGCAAAAAAGCTCCCGAAGGAGCCTTCATTTTCACTTTTTTAAATCCAACGACAGACGGCTGGCAGTTAAGTATTGTGAAATATTATCAAATg < 2:844770/151‑1 (MQ=255) tATGATTTTTTTCATGATGTTACCGTAGTATGTGAGTATCCATCGAATAGACACCAAGCAAAAAAGCTCCCGAAGGAGCCTTCATTTTCACTTTTTTAAATCCAACGACAGACGGCTGGCAGTTAAGTATTGTGAAATATTATCAAATGt > 1:398621/1‑150 (MQ=255) aTGATTTTTTTCATGATGTTACCGTAGTATGTGAGTATCCATCGAATAGACACCAAGCAAAAAAGCTCCCGAAGGAGCCTTCATTTTCACTTTTTTAAATCCAACGACAGACGGCTGGCAGTTAAGTATTGTGAAATATTATCAAATGTaa < 2:698499/151‑1 (MQ=255) aTGATTTTTTTCATGATGTTACCGTAGTATGTGAGTATCCATCGAATAGACACCAAGCAAAAAAGCTCCCGAAGGAGCCTTCATTTTCACTTTTTTAAATCCAACGACAGACGGCTGGCAGTTAAGTATTGTGAAATATTATCAAATGTa < 2:475244/150‑1 (MQ=255) aTTTTTTTCATGATGTTACCGTAGTATGTGAGTATCCATCGAATAGACACCAAGCAAAAAAGCTCCCGAAGGAGCCTTCATTTTCACTTTTTTAAATCCAACGACAGACGGCTGGCAGTTAAGTATTGTGAAATATTATCAAATGTAatc > 2:125617/1‑150 (MQ=255) tttCATGATGTTACCGTAGTATGTGAGTATCCATCGAATAGACACCAAGCAAAAAAGCTCCCGAAGGAGCCTTCATTTTCACTTTTTTAAATCCAACGACAGACGGCTGGCAGTTAAGTATTGTGAAATATTATCAAATGTAATCATCAtt > 1:784422/1‑151 (MQ=255) ttCATGATGTTACCGTAGTATGTGAGTATCCATCGAATAGACACCAAGCAAAAAAGCTCCCGAAGGAGCCTTCATTTTCACTTTTTTAAATCCAACGACAGACGGCTGGCAGTTAAGTATTGTGAAATATTATCAAATGTAATCATCAtt < 2:785838/150‑1 (MQ=255) tCATGATGTTACCGTAGTATGTGAGTATCCATCGAATAGACACCAAGCAAAAAAGCTCCCGAAGGAGCCTTCATTTTCACTTTTTTAAATCCAACGACAGACGGCTGGCAGTTAAGTATTGTGAAATATTATCAAATGTAATCATCATTg > 1:488317/1‑150 (MQ=255) cATGATGTTACCGTAGTATGTGAGTATCCATCGAATAGACACCAAGCAAAAAAGCTCCCGAAGGAGCCTTCATTTTCACTTTTTTAAATCCAACGACAGACGGCTGGCAGTTAAGTATTGTGAAATATTATCAAATGTAATCATCATTGAt < 2:708618/151‑1 (MQ=255) gatgTTACCGTAGTATGTGAGTATCCATCGAATAGACACCAAGCAAAAAAGCTCCCGAAGGAGCCTTCATTTTCACTTTTTTAAATCCAACGACAGACGGCTGGCAGTTAAGTATTGTGAAATATTATCAAATGTAATCATCATTGATTTa < 1:659167/151‑1 (MQ=255) gatgTTACCGTAGTATGTGAGTAGCCATCGAATAGACACCAAGCAAAAAAGCTCCCGAAGGAGCCTTCATTTTCACTTTTTTAAATCCAACGACAGACGGCTGCCAGTTAAGTATTGTGAAATATTATCAAATGTAATCATCATTGAttt < 2:229544/150‑1 (MQ=255) tACCGTAGTATGTGAGTATCCATCGAATAGACACCAAGCAAAAAAGCTCCCGAAGGAGCCTTCATTTTCACTTTTTTAAATCCAACGACAGACGGCTGGCAGTTAAGTATTGTGAAATATTATCAAATGTAATCATCATTGATTTACaaa < 1:791891/150‑1 (MQ=255) aTGTGAGTATCCATCGAATAGACACCAAGCAAAAAAGCTCCCGAAGGAGCCTTCATTTTCACTTTTTTAAATCCAACGACAGACGGCTGGCAGTTAAGTATTGTGAAATATTATCAAATGTAATCATCATTGATTTACAAAAGATACAttt > 2:511619/1‑151 (MQ=255) aTGTGAGTATCCATCGAATAGACACCAAGCAAAAAAGCTCCCGAAGGAGCCTTCATTTTCACTTTTTTAAATCCAACGACAGACGGCTGGCAGTTAAGTATTGTGAAATATTATCAAATGTAATCATCATTGATTTACAAAAGATACAtt < 1:500372/150‑1 (MQ=255) gtgAGTATCCATCGAATAGACACCAAGCAAAAAAGCTCCCGAAGGAGCCTTCATTTTCACTTTTTTAAATCCAACGACAGACGGCTGGCAGTTAAGTATTGTGAAATATTATCAAATGTAATCATCATTGATTTACAAAAGATACAtttt < 1:686983/150‑1 (MQ=255) gAGTATCCATCGAATAGACACCAAGCAAAAAAGCTCCCGAAGGAGCCTTCATTTTCACTTTTTTAAATCCAACGACAGACGGCTGGCAGTTAAGTATTGTGAAATATTATCAAATGTAATCATCATTGATTTACAAAAGATACATTTTGcc > 1:917919/1‑151 (MQ=255) aTCCATCGAATAGACACCAAGCAAAAAAGCTCCCGAAGGGGCCTTCATTTTCACTTTTTTAAATCCAACGACAGACGGTTGGCAGTTAAGTATTGTGAATTATTATCAAATGTAATCATCATTGATTTACAAAAGATACATTTTGCCCCg > 2:418209/1‑150 (MQ=255) ccATCGAATAGACACCAAGCAAAAAAGCTCCCGAAGGAGCCTTCATTTTCACTTTTTTAAATCCAACGACAGACGGCTGGCAGTTAAGTATTGTGAAATATTATCAAATGTAATCATCATTGATTTACAAAAGATACATTTTGCCCCGaa < 2:574667/150‑1 (MQ=255) tCGAATAGACACCAAGCAAAAAAGCTCCCGAAGGAGCCTTCATTTTCACTTTTTTAAATCCAACGACAGACGGCTGGCAGTTAAGTATTGTGAAATATTATCAAATGTAATCATCATTGATTTACAAAAGATACATTTTGCCCCGAAAgg > 1:850389/1‑150 (MQ=255) cGAATAGACACCAAGCAAAAAAGCTCCCGAAGGAGCCTTCATTTTCACTTTTTTAAATCCAACGACAGACGGCTGGCAGTTAAGTATTGTGAAATATTATCAAATGTAATCATCATTGATTTACAAAAGATACATTTTACCCCGAAAGGAt > 1:203801/1‑151 (MQ=255) aGACACCAAGCAAAAAAGCTCCCGAAGGAGCCTTCATTTTCACTTTTTTAAATCCAACGACAGACGGCTGGCAGTTAAGTATTGTGAAATATTATCAAATGTAATCATCATTGATTTACAAAAGATACATTTTGCCCCGAAAGGATTCAt < 2:662448/150‑1 (MQ=255) aGACACCAAGCAAAAAAGCTCCCGAAGGAGCCTTCATTTTCACTTTTTTAAATCCAACGACAGACGGCTGGCAGTTAAGTATTGTGAAATATTATCAAATGTAATCATCATTGATTTACAAAAGATACATTTTGCCCCGAAAGGATTCAt < 2:356799/150‑1 (MQ=255) ccAAGCAAAAAAGCTCCCGAAGGAGCCTTCATTTTCACTTTTTTAAATCCAACGACAGACGGCTGGCAGTTAAGTATTGTGAAATATTATCAAATGTAATCATCATTGATTTACAAAAGATACATTTTGCCCCGAAAGGATTCATGTc > 2:678179/1‑148 (MQ=255) aGCAAAAAAGCTCCCGAAGGAGCCTTCATTTTCACTTTTTTAAATCCAACGACAGACGGCTGGCAGTTAAGTATTGTGAAATATTATCAAATGTAATCATCATTGATTTACAAAAGATACATTTTGCCCCGAAAGGATTCATGTCAGAAAc < 2:361047/151‑1 (MQ=255) ataaaaTCTCCCGGAGGAGCCTTCATTTTCACTTTTTTAAATCCTACGACAGACGGCTGGCAATTAAGTATTGTGAAATATTAGCCAATGTAATCAGCATTGATTTACAAAAGATACATTTTGCCCTGAAAGGATTCATGTCAGAAACa < 1:77677/147‑1 (MQ=255) cccGAAGGAGCCTTCATTTTCACTTTTTTAAATCCAACGACAGACGGCTGGCAGTTAAGTATTGTGAAATATTATCAAATGTAATCATCATTGATTTACAAAAGATACATTTTGCCCCGAAAGGATTCATGTCAGAAACATCAAAAgatg > 1:777836/1‑150 (MQ=255) ccGAAGGAGCCTTCATTTTCACTTTTTTAAATCCAACGACAGACGGCTGGCAGTTAAGTATTGTGAAATATTATCAAATGTAATCATCATTGATTTACAAAAGATACATTTTGCCCCGAAAGGATTCATGTCAGAAACATCAAAAgatgat > 2:408344/1‑151 (MQ=255) aaGGAGCCTTCATTTTCACTTTTTTAAATCCAACGACAGACGGCTGGCAGTTAAGTATTGTGAAATATTATCAAATGTAATCATCATTGATTTACAAAAGATACATTTTGCCCCGAAAGGATTCATGTCAGAAACATCAAAAGATGATGtt > 2:544931/1‑151 (MQ=255) aaGGAGCATTCATTTTCACTTTTTTAAATCCAACGACAGACGGCTGGCAGTTAAGTATTGTGAAATATTATCAAATGTAATCATCATTGATTTACAAAAGATACATTTTGCCCCGAAAGGATTCATGTCAGAAACATCAAAAGATGATGtt > 1:761266/1‑151 (MQ=255) ggAGCCTTCATTTTCACTTTTTTAAATCCAACGACAGACGGCTGGCAGTTAAGTATTGTGAAATATTATCAAATGTAATCATCATTGATTTACAAAAGATACATTTTGCCCCGAAAGGATTCATGTCAGAAACATCAAAAGATGATGTTCt > 1:508785/1‑151 (MQ=255) cTTCATTTTCACTTTTTTAAATCCAACGACAGACGGCTGGCAGTTAAGTATTGTGAAATATTATCAAATGTAATCATCATTGATTTACAAAAGATACATTTTGCCCCGAAAGGATTCATGTCAGAAACATCAAAAGATGATGTTCTATACt > 1:615228/1‑151 (MQ=255) ttCATTTTCACTTTTTTAAATCCAACGACAGACGGCTGGCAGTTAAGTATTGTGAAATATTATCATATGTAATCATCATTGATTTACAAAAGATACATTTTGCCCCGAAAGGATTCATGTCAGAAACATCAAAAGATGATGTTCTATACTg > 2:90665/1‑151 (MQ=255) tCATTTTCACTTTTTTAAATCCAACGACAGACGGCTGGCAGTTAAGTATTGTGAAATATTATCAAATGTAATCATCATTGATTTACAAAAGATACATTTTGCCCCGAAAGGATTCATGTCAGAAACATCAAAAGATGATGTTCTATACTg < 2:33293/150‑1 (MQ=255) cATTTTCACTTTTTTAAATCCAACGACAGACGGCTGGCAGTTAAGTATTGTGAAATATTATCAAATGTAATCATCATTGATTTACAAAAGATACATTTTGCCCCGAAAGGATTCATGTCAGAAACATCAAAAGATGATGTTCTATACTgg < 1:381131/150‑1 (MQ=255) aCTTTTTTAAATCCAACGACAGACGGCTGGCAGTTAAGTATTGTGAAATATTATCAAATGTAATCATCATTGATTTACAAAAGATACATTTTGCCCCGAAAGGATTCATGTCAGAAACATCAAAAGATGATGTTCTATACTGGGAACCATg < 1:221838/151‑1 (MQ=255) ttttAAATCCAACGACAGACGGCTGGCAGTTAAGTATTGTGAAATATTATCAAATGTAATCATCATTGATTTACAAAAGATACATTTTGCCCCGAAAGGATTCATGTCAGAAACATCAAAAGATGATGTTCTATACTGGGAACCATGaca < 1:773936/150‑1 (MQ=255) tttAAATCCAACGACAGACGGCTGGCATTTAAGTATTGTGAAATATTATCAAATGTAATCATCATTGATTTACAAAAGATACATTTTGCCCCGAAAGGATTCATGTCAGAAACATCAAAAGATGATGTTCTATACTGGGAACCATGacaa < 2:790759/150‑1 (MQ=255) tAAATCCAACGACAGACGGCTGGCAGTTAAGCATTGTGAAAAAGTATCAACTGTAATCATCATTGATTTACAAAAGATACATTTTGCCCCGAAAGGATTCATGTCAGAAACATCAAAAGATGATGTTCTATACTGGGAACCATGACAacac > 1:746242/1‑151 (MQ=255) tCCAACGACAGACGGCTGGCAGTTAAGTATTGTGAAATATTATCAAATGTAATCATCATTGATTTACAAAAGATACATTTTGCCCCGAAAGGATTCATGTCAGAAACATCAAAAGATGATGTTCTATACTGGGAACCATGACAACACGGGc > 1:175700/1‑151 (MQ=255) ccAACGACAGACGGCTGGCAGTTAAGTATTGTGAAATATTATCAAATGTAATCATCATTGATTTACAAAAGATACATTTTGCCCCGAAAGGATTCATGTCAGAAACATCAAAAGATGATGTTCTATACTGGGAACCATGACAACACGGGCa < 2:258632/151‑1 (MQ=255) aaCGACAGACGGCTGGCAGTTAAGTATTGTGAAATATTATCAAATGTAATCATCATTGATTTACAAAAGATACATTTTGCCCCGAAAGGATTCATGTCAGAAACATCAAAAGATGATGTTCTATACTGGGAACCATGACAACACGGGCATc > 1:640048/1‑151 (MQ=255) aGACGGCTGGCAGTTAAGTATTGTGAAATATTATCAAATGTAATCAACATTGATTTACAAAAGATACATTTTGCCCCGAAAGGATTCATGTCAGAAACATCAAAAGATGATGTTCTATACTGGGAACCATGACAACACGGGCATCTAAAGt > 2:465686/1‑151 (MQ=255) cGGCTGGCAGTTAAGTATTGTGAAATATTATCAAATGTAATCATCATTGATTTACAAAAGATACATTTTGCCCCGAAAGGATTCATGTCAGAAACATCAAAAGATGATGTTCTATACTGGGAACCATGACAACACGGGCATCTAAAGTGa > 2:930232/1‑150 (MQ=255) cTGGCAGTTAAGTATTGTGAAATATTATCAAATGTAATCATCATTGATTTACAAAAGATACATTTTGCCCCGAAAGGATTCATGTCAGAAACATCAAAAGATGATGTTCTATACTGGGAACCATGACAACACGGGCATCTAAAGTGAata > 1:933638/1‑150 (MQ=255) gCAGTTAAGTATTGTGAAATATTATCAAATGTAATCATCATTGATTTACAAAAGATACATTTTGCCCCGAAAGGATTCATGTCAGAAACATCAAAAGATGATGTTCTATACTGGGAACCATGACAACACGGGCATCTAAAGTGAATATGGt < 1:708732/151‑1 (MQ=255) | GCTGGTTCAGAGTTAAAAAAACTATGATTTTTTTCATGATGTTACCGTAGTATGTGAGTATCCATCGAATAGACACCAAGCAAAAAAGCTCCCGAAGGAGCCTTCATTTTCACTTTTTTAAATCCAACGACAGACGGCTGGCATTTAAGTATTGTGAAATATTATCAAATGTAATCATCATTGATTTACAAAAGATACATTTTGCCCCGAAAGGATTCATGTCAGAAACATCAAAAGATGATGTTCTATACTGGGAACCATGACAACACGGGCATCTAAAGTGAATATGGT > NC_000913/1638147‑1638437 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |