Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,704,474	T→G	intergenic (‑166/‑77)	ydgJ ← / → blr	putative oxidoreductase/beta‑lactam resistance membrane protein; divisome‑associated protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,704,474	0	T	G	100.0%	174.5 / NA	48	intergenic (‑166/‑77)	ydgJ/blr	putative oxidoreductase/beta‑lactam resistance membrane protein; divisome‑associated protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (26/22);  total (26/22)

GTATGATGGCTATTTGACACTATCCTTTACCCACGCTCAACAGTTTAATAACCTGCCAGCAATAAGGGATGTTGTTTAACTTAAGTCAAAAAAATAGCGAATTTTCCAACGACAAAAGCTAAATATCGCAAAAACCTCAGTAAAAATCTTGCTGGAGCTATTATTGCTAAGTAACATTTACCCCCTGAAGTTAATGGATCAATCAAGAGAGATGTGGGCTGTAATGAATCGTCTTATTGAATTAACAGGTTGGATCGTTCTTGTCGTTTCAGTCATTCTTCTTGGCGTGGCG  >  NC_000913/1704328‑1704619                                                                                                                                                   |                                                                                                                                                  gTATGATGGCTATTTGACACTATCCTTTACCCACGCTCAACAGTTTAATAACCTGCCAGCAATAAGGGATGTTGTTTAACTTAAGTCAAAAAAATAGCGAATTTTCCAACGACAAAAGCTAAATATCGCAAAAACCTCAGTAAAAAGCTTg                                                                                                                                               <  1:188184/151‑1 (MQ=255)  tATGATGGCTATTTGACACTATCCTTTACCCACGCTCAACAGTTTAATAACCTGCCAGCAATAAGGGATGTTGTTTAACTTAAGTCAAAAAAATAGCGAATTTTCCAACGACAAAAGCTAAATATCGCAAAAACCTCAGTAAAAAGCTTg                                                                                                                                               <  1:478569/150‑1 (MQ=255)   atgatgGCTATTTGACACTATCCTTTACCCACGCTCAACAGTTTAATAACCTGCCAGCAATAAGGGATGTTGTTTAACTTAAGTCAAAAAAATAGCGAATTTTCCAACGACAAAAGCTAAATATCGCAAAAACCTCAGTAAAAAGCTTGCt                                                                                                                                             >  1:878210/1‑151 (MQ=255)   atgatgGCTATTTGACACTATCCTTTACCCACGCTCAACAGTTTAATAACCTGCCAGCAATAAGGGATGTTGTTTAACTTAAGTCAAAAAAATAGCGAATTTTCCAACGACAAAAGCTAAATATCGCAAAAACCTCAGTAAAAAGCTTGCt                                                                                                                                             >  2:33974/1‑151 (MQ=255)    tgatgGCTATTTGACACTATCCTTTACCCACGCTCAACAGTTTAATAACCTGCCAGCAATAAGGGATGTTGTTTAACTTAAGTCAAAAAAATAGCGAATTTTCCAACGACAAAAGCTAAATATCGCAAAAACCTCAGTAAAAAGCTTGCt                                                                                                                                             >  1:724608/1‑150 (MQ=255)         gCTATTTGACACTATCCTTTACCCACGCTCAACAGTTTAATAACCTGCCAGCAATAAGGGATGTTGTTTAACTTAAGTCAAAAAAATAGCGAATTTTCCAACGACAAAAGCTAAATATCGCAAAAACCTCAGTAAAAAGCTTGCTGGAGCt                                                                                                                                       >  1:599590/1‑151 (MQ=255)                gACACTATCCTTTACCCACGCTCAACAGTTTAATAACCTGCCAGCAATAAGGGATGTTGTTTAACTTAAGTCAAAAAAATAGCGAATTTTCCAACGACAAAAGCTAAATATCGCAAAAACCTCAGTAAAAAGCTTGCTGGAGCTATTATTg                                                                                                                                <  2:158699/151‑1 (MQ=255)                     tATCCTTTACCCACGCTCAACAGTTTAATAACCTGCCAGCAATAAGGGATGTTGTTTAACTTAAGTCAAAAAAATAGCGAATTTTCCAACGACAAAAGCTAAATATCGCAAAAACCTCAGTAAAAAGCTTGCTGGAGCTATTATTGCTa                                                                                                                             >  1:270771/1‑149 (MQ=255)                     tATCCTTTACCCACGCTCAACAGTTTAATAACCTGCCAGCAATAAGGGATGTTGTTTAACTTAAGTCAAAAAAATAGCGAATTTTCCAACGACAAAAGCTAAATATCGCAAAAACCTCAGTAAAAAGCTTGCTGGAGCTATTATTGCTa                                                                                                                             >  1:895409/1‑149 (MQ=255)                      aTCCTTTACCCACGCTCAACAGTTTAATAACCTGCCAGCAATAAGGGATGTTGTTTAACTTAAGTCAAAAAAATAGCGAATTTTCCAACGACAAAAGCTAAATATCGCAAAAACCTCAGTAAAAAGCTTGCTGGAGCTATTATTGCTaa                                                                                                                            <  1:715311/149‑1 (MQ=255)                      aTCCTTTACCCACGCTCAACAGTTTAATAACCTGCCAGCAATAAGGGATGTTGTTTAACTTAAGTCAAAAAAATAGCGAATTTTCCAACGACAAAAGCTAAATATCGCAAAAACCTCAGTAAAAAGCTTGCTGGAGCTATTATTGCTAAg                                                                                                                           >  1:314137/1‑150 (MQ=255)                          tttACCCACGCTCAACAGTTTAATAACCTGCCAGCAATAAGGGATGTTGTTTAACTTAAGTCAAAAAAATAGCGAATTTTCCAACGACAAAAGCTAAATATCGCAAAAACCTCAGTAAAAAGCTTGCTGGAGCTATTATTGCTAAGTAAc                                                                                                                       >  2:559271/1‑150 (MQ=255)                           ttACCCACGCTCAACAGTTTAATAACCTGCCAGCAATAAGGGATGTTGTTTAACTTAAGTCAAAAAAATAGCGAATTTTCCAACGACAAAAGCTAAATATCGCAAAAACCTCAGTAAAAAGCTTGCTGGAGCTATTATTGCTAAGTAAc                                                                                                                       <  1:549261/149‑1 (MQ=255)                             aCCCACGCTCAACAGTTTAATAACCTGCCAGCAATAAGGGATGTTGTTTAACTTAAGTCAAAAAAATAGCGAATTTTCCAACGACAAAAGCTAAATATCGCAAAAACCTCAGTAAAAAGCTTGCTGGAGCTATTATTGCTAAGTAACAttt                                                                                                                   <  2:62595/151‑1 (MQ=255)                                      cAACAGTTTAATAACCTGCCAGCAATAAGGGATGTTGTTTAACTTAAGTCAAAAAAATAGCGAATTTTCCAACGACAAAAGCTAAATATCGCAAAAACCTCAGTAAAAAGCTTGCTGGAGCTATTATTGCTAAGTAACATTTACCCCCTGa                                                                                                          <  1:687406/151‑1 (MQ=255)                                         cAGTTTAATAACCTGCCAGCAATAAGGGATGTTGTTTAACTTAAGTCAAAAAAATAGCGAATTTTCCAACGACAAAAGCTAAATATCGCAAAAACCTCAGTAAAAAGCTTGCTGGAGCTATTATTGCTAAGTAACATTTACCCCCTGAAGt                                                                                                       <  1:36851/151‑1 (MQ=255)                                           gTTTAATAACCTGCCAGCAATAAGGGATGTTGTTTAACTTAAGTCAAAAAAATAGCGAATTTTCCAACGACAAAAGCTAAATATCGCAAAAACCTCAGTAAAAAGCTTGCTGGAGCTATTATTGCTAAGTAACATTTACCCCCTGAAGTTa                                                                                                     <  2:51221/151‑1 (MQ=255)                                           gTTTAATAACCTGCCAGCAATAAGGGATGTTGTTTAACTTAAGTCAAAAAAATAGCGAATTTTCCAACGACAAAAGCTAAATATCGCAAAAACCTCAGTAAAAAGCTTGCTGGAGCTATTATTGCTAAGTAACATTTACCCCCTGAAGTTa                                                                                                     <  1:846181/151‑1 (MQ=255)                                            tttAATAACCTGCCAGCAATAAGGGATGTTGTTTAACTTAAGTCAAAAAAATAGCGAATTTTCCAACGACAAAAGCTAAATATCGCAAAAACCTCAGTAAAAAGCTTGCTGGAGCTATTATTGCTAAGTAACATTTACCCCCTGAAGTTa                                                                                                     >  2:181173/1‑150 (MQ=255)                                                 taaCCTGCCAGCAATAAGGGATGTTGTTTAACTTAAGTCAAAAAAATAGCGAATTTTCCAACGACAAAAGCTAAATATCGCAAAAACCTCAGTAAAAAGCTTGCTGGAGCTATTATTGCTAAGTAACATTTACCCCCTGAAGTTAATGGat                                                                                               >  2:245211/1‑151 (MQ=255)                                                  aaCCTGCCAGCAATAAGGGATGTTGTTTAACTTAAGTCAAAAAAATAGCGAATTTTCCAACGACAAAAGCTAAATATCGCAAAAACCTCAGTAAAAAGCTTGCTGGAGCTATTATTGCTAAGTAACATTTACCCCCTGAAGTTAATGGat                                                                                               <  1:739906/150‑1 (MQ=255)                                                   aCCTGCCAGCAATAAGGGATGTTGTTTAACTTAAGTCAAAAAAATAGCGAATTTTCCAACGACAAAAGCTAAATATCGCAAAAACCTCAGTAAAAAGCTTGCTGGAGCTATTATTGCTAAGTAACATTTACCCCCTGAAGTTAATGGatc                                                                                              >  1:568709/1‑150 (MQ=255)                                                       gCCAGCAATAAGGGATGTTGTTTAACTTAAGTCAAAAAAATAGCGAATTTTCCAACGACAAAAGCTAAATATCGCAAAAACCTCAGTAAAAAGCTTGCTGGAGCTATTATTGCTAAGTAACATTTACCCCCTGAAGTTAATGGatcaatca                                                                                         <  2:113599/151‑1 (MQ=255)                                                       gCCAGCAATAAGGGATGTTGTTTAACTTAAGTCAAAAAAATAGCGAATTTTCCAACGACAAAAGCTAAATATCGCAAAAACCTCAGTAAAAAGCTTGCTGGAGCTATTATTGCTAAGTAACATTTACCCCCTGAAGTTAATGGatcaatc                                                                                          <  1:887463/150‑1 (MQ=255)                                                           gCAATAAGGGATGTTGTTTAACTTAAGTCAAAAAAATAGCGAATTTTCCAACGACAAAAGCTAAATATCGCAAAAACCTCAGTAAAAAGCTTGCTGGAGCTATTATTGCTAAGTAACATTTACCCCCTGAAGTTAATGGATCAATCAag                                                                                       >  2:927127/1‑149 (MQ=255)                                                             aaTAAGGGATGTTGTTTAACTTAAGTCAAAAAAATAGCGAATTTTCCAACGACAAAAGCTAAATATCGCAAAAACCTCAGTAAAAAGCTTGCTGGAGCTATTATTGCTAAGTAACATTTACCCCCTGAAGTTAATGGATCAATCAagag                                                                                     >  2:523360/1‑149 (MQ=255)                                                                aaGGGATGTTGTTTAACTTAAGTCAAAAAAATAGCGAATTTTCCAACGACAAAAGCTAAATATCGCAAAAACCTCAGTAAAAAGCTTGCTGGAGCTATTATTGCTAAGTAACATTTACCCCCTGAAGTTAATGGATCAATCAAGAGAGAtg                                                                                >  1:792614/1‑151 (MQ=255)                                                                  gggATGTTGTTTAACTTAAGTCAAAAAAATAGCGAATTTTCCAACGACAAAAGCTAAATATCGCAAAAACCTCAGTAAAAAGCTTGCTGGAGCTATTATTGCTAAGTAACATTTACCCCCTGAAGTTAATGGATCAATCAAGAGAGAtgtg                                                                              >  1:919912/1‑151 (MQ=255)                                                                    gATGTTGTTTAACTTAAGTCAAAAAAATAGCGAATTTTCCAACGACAAAAGCTAAATATCGCAAAAACCTCAGTAAAAAGCTTGCTGGAGCTATTATTGCTAAGTAACATTTACCCCCTGAAGTTAATGGATCAATCAAGAGAGATGTggg                                                                            <  1:4493/151‑1 (MQ=255)                                                                       gttgttTAACTTAAGTCAAAAAAATAGCGAATTTTCCAACGACAAAAGCTAAATATCGCAAAAACCTCAGTAAAAAGCTTGCTGGAGCTATTATTGCTAAGTAACATTTACCCCCTGAAGTTAATGGATCAATCAAGAGAGATGTGGGCt                                                                          <  2:173444/150‑1 (MQ=255)                                                                        ttgttTAACTTAAGTCAAAAAAATAGCGAATTTTCCAACGACAAAAGCTAAATATCGCAAAAACCTCAGTAAAAAGCTTGCTGGAGCTATTATTGCTAAGTAACATTTACCCCCTGAAGTTAATGGATCAATCAAGAGAGATGTGGGCTGt                                                                        >  2:678376/1‑151 (MQ=255)                                                                         tgttTAACTTAAGTCAAAAAAATAGCGAATTTTCCAACGACAAAAGCTAAATATCGCAAAAACCTCAGTAAAAAGCTTGCTGGAGCTATTATTGCTAAGTAACATTTACCCCCTGAAGTTAATGGATCAATCAAGAGAGATGTGGGCTGt                                                                        >  2:16852/1‑150 (MQ=255)                                                                         tgttTAACTTAAGTCAAAAAAATAGCGAATTTTCCAACGACAAAAGCTAAATATCGCAAAAACCTCAGTAAAAAGCTTGCTGGAGCTATTATTGCTAAGTAACATTTACCCCCTGAAGTTAATGGATCAATCAAGAGAGATGTGGGCTGt                                                                        >  1:565651/1‑150 (MQ=255)                                                                                  tAAGTCAAAAAAATAGCGAATTTTCCAACGACAAAAGCTAAATATCGCAAAAACCTCAGTAAAAAGCTTGCTGGAGCTATTATTGCTAAGTAACATTTACCCCCTGAAGTTAATGGATCAATCAAGAGAGATGTGGGCTGTAATGAATc                                                                <  1:537570/149‑1 (MQ=255)                                                                                        aaaaaaaTAGCGAATTTTCCAACGACAAAAGCTAAATATCGCAAAAACCTCAGTAAAAAGCTTGCTGGAGCTATTATTGCTAAGTAACATTTACCCCCTGAAGTTAATGGATCAATCAAGAGAGATGTGGGCTGTAATGAATCGTCTTAtt                                                        <  2:272851/151‑1 (MQ=255)                                                                                                   gAATTTTCCAACGACAAAAGCTAAATATCGCAAAAACCTCAGTAAAAAGCTTGCTGGAGCTATTATTGCTAAGTAACATTTACCCCCTGAAGTTAATGGATCAATCAAGAGAGATGTGGGCTGTAATGAATCGTCTTATTGAATTAACAgg                                             <  1:128751/151‑1 (MQ=255)                                                                                                         tCCAACGACAAAAGCTAAATATCGCAAAAACCTCAGTAAAAAGCTTGCTGGAGCTATTATTGCTAAGTAACATTTACCCCCTGAAGTTAATGGATCAATCAAGAGAGATGTGGGCTGTAATGAATCGTCTTATTGAATTAACAGGTTGGa                                        <  2:733782/150‑1 (MQ=255)                                                                                                               gACAAAAGCTAAATATCGCAAAAACCTCAGTAAAAAGCTTGCTGGAGCTATTATTGCTAAGTAACATTTACCCCCTGAAGTTAATGGATCAATCAAGAGAGATGTGGGCTGTAATGAATCGTCTTATTGAATTAACAGGTTGGATCGTTCt                                 <  1:928076/151‑1 (MQ=255)                                                                                                                 cAAAAGCTAAATATCGCAAAAACCTCAGTAAAAAGCTTGCTGGAGCTATTATTGCTAAGTAACATTTACCCCCTGAAGTTAATGGATCAATCAAGAGAGATGTGGGCTGTAATGAATCGTCTTATTGAATTAACAGGTTGGATCGTTCTTg                               >  1:733551/1‑151 (MQ=255)                                                                                                                   aaaGCTAAATATCGCAAAAACCTCAGTAAAAAGCTTGCTGGAGCTATTATTGCTAAGTAACATTTACCCCCTGAAGTTAATGGATCAATCAAGAGAGATGTGGGCTGTAATGAATCGTCTTATTGAATTAACAGGTTGGATCGTTCTTGTc                             >  2:27579/1‑151 (MQ=255)                                                                                                                     aGCTAAATATCGCAAAAACCTCAGTAAAAAGCTTGCTGGAGCTATTATTGCTAAGTAACATTTACCCCCTGAAGTTAATGGATCAATCAAGAGAGATGTGGGCTGTAATGAATCGTCTTATTGAATTAACAGGTTGGATCGTTCTTGTCGt                           <  2:270779/151‑1 (MQ=255)                                                                                                                         aaaTATCGCAAAAACCTCAGTAAAAAGCTTGCTGGAGCTATTATTGCTAAGTAACATTTACCCCCTGAAGTTAATGGATCAATCAAGAGAGATGTGGGCTGTAATGAATCGTCTTATTGAATTAACAGGTTGGATCGTTCTTGTCGTTTc                        >  2:181361/1‑150 (MQ=255)                                                                                                                                 cAAAAACCTCAGTAAAAAGCTTGCTGGAGCTATTATTGCTAAGTAACATTTACCCCCTGAAGTTAATGGATCAATCAAGAGAGATGTGGGCTGTAATGAATCGTCTTATTGAATTAACAGGTTGGATCGTTCTTGTCGTTTCAGTCAttct               >  2:266193/1‑151 (MQ=255)                                                                                                                                 cAAAAACCTCAGTAAAAAGCTTGCTGGAGCTATTATTGCTAAGTAACATTTACCCCCTGAAGTTAATGGATCAATCAAGAGAGATGTGGGCTGTAATGAATCGTCTTATTGAATTAACAGGTTGGATCGTTCTTGTCGTTTCAGTCAttct               >  1:127041/1‑151 (MQ=255)                                                                                                                                   aaaaCCTCAGTAAAAAGCTTGCTGGAGCTATTATTGCTAAGTAACATTTACCCCCTGAAGTTAATGGATCAATCAAGAGAGATGTGGGCTGTAATGAATCGTCTTATTGAATTAACAGGTTGGATCGTTCTTGTCGTTTCAGTCAttcttc             >  1:34449/1‑151 (MQ=255)                                                                                                                                        cTCAGTAAAAAGCTTGCTGGAGCTATTATTGCTAAGTAACATTTACCCCCTGAAGTTAATGGATCAATCAAGAGAGATGTGGGCTGTAATGAATCGTCTTATTGAATTAACAGGTTGGATCGTTCTTGTCGTTTCAGTCATTCTTCTtggc        >  2:283647/1‑151 (MQ=255)                                                                                                                                            gTAAAAAGCTTGCTGGAGCTATTATTGCTAAGTAACATTTACCCCCTGAAGTTAATGGATCAATCAAGAGAGATGTGGGCTGTAATGAATCGTCTTATTGAATTAACAGGTTGGATCGTTCTTGTCGTTTCAGTCATTCTTCTtggcgtgg    <  2:819861/151‑1 (MQ=255)                                                                                                                                               aaaaGCTTGCTGGAGCTATTATTGCTAAGTAACATTTACCCCCTGAAGTTAATGGATCAATCAAGAGAGATGTGGGCTGTAATGAATCGTCTTATTGAATTAACAGGTTGGATCGTTCTTGTCGTTTCAGTCATTCTTCTtggcgtggcg  >  2:28901/1‑150 (MQ=255)                                                                                                                                                   |                                                                                                                                                  GTATGATGGCTATTTGACACTATCCTTTACCCACGCTCAACAGTTTAATAACCTGCCAGCAATAAGGGATGTTGTTTAACTTAAGTCAAAAAAATAGCGAATTTTCCAACGACAAAAGCTAAATATCGCAAAAACCTCAGTAAAAATCTTGCTGGAGCTATTATTGCTAAGTAACATTTACCCCCTGAAGTTAATGGATCAATCAAGAGAGATGTGGGCTGTAATGAATCGTCTTATTGAATTAACAGGTTGGATCGTTCTTGTCGTTTCAGTCATTCTTCTTGGCGTGGCG  >  NC_000913/1704328‑1704619

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑