Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,736,170	A→C	F374C (TTT→TGT)	ydhP ←	putative MFS transporter, inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,736,170	0	A	C	100.0%	182.6 / NA	50	F374C (TTT→TGT)	ydhP	putative MFS transporter, inner membrane protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (30/20);  total (30/20)

GTAGGTCGGATAAGGCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTCTGGCTGACATAAACACCAGCAATAATGCCAGTCCCGCGACAATCGCTCCCATCACCGGCACAAAGCTGTATCCCAGCCCAGCGGAAATTACCGCACCACCAGCAGCTGCTCCCAGCGCATTTCCAAGATTAAAGGCACCAATATTGACTGATGAA  >  NC_000913/1736025‑1736313                                                                                                                                                  |                                                                                                                                                gTAGGTCGGATAAGGCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTCTGGCTGACATacacac                                                                                                                                             >  2:26964/1‑150 (MQ=255)         gATAAGGCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTCTGGCTGACATACACACCAGCaataa                                                                                                                                    <  2:472423/151‑1 (MQ=255)            aaGGCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTCTGGCTGACATACACACCAGCAATAATGc                                                                                                                                 <  2:329592/151‑1 (MQ=255)             aGGCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTCTGGCTGACATACACACCAGCAATAATGcc                                                                                                                                >  1:307662/1‑151 (MQ=255)                cACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTCTGGCTGACATACACACCAGCAATAATGCCAGt                                                                                                                             >  2:247713/1‑151 (MQ=255)                 aCTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTCTGGCTGACATACACACCAGCAATAATGCCAGTc                                                                                                                            >  2:634740/1‑151 (MQ=255)                    cgccgcATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTCTGGCTGACATACACACCAGCAATAATGCCAGTcc                                                                                                                           <  2:712779/149‑1 (MQ=255)                        gcATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTCTGGCTGACATACACACCAGCAATAATGCCAGTCCCGCGAc                                                                                                                     >  1:568695/1‑151 (MQ=255)                         cATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTCTGGCTGACATACACACCAGCAATAATGCCAGTCCCGCGAc                                                                                                                     >  2:154009/1‑150 (MQ=255)                          aTCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTCTGGCTGACATACACACCAGCAATAATGCCAGTCCCGCGACaa                                                                                                                   <  1:662339/151‑1 (MQ=255)                            ccGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTCTGGCTGACATACACACCAGCAATAATGCCAGTCCCGCGACAATc                                                                                                                 <  1:4600/151‑1 (MQ=255)                                    aTAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTCTGGCTGACATACACACCAGCAATAATGCCAGTCCCGCGACAATCGCTCCCAt                                                                                                         >  2:189395/1‑151 (MQ=255)                                    aTAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTCTGGCTGACATACACACCAGCAATAATGCCAGTCCCGCGACAATCGCTCCCAt                                                                                                         >  1:439466/1‑151 (MQ=255)                                       aaTCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTCTGGCTGACATACACACCAGCAATAATGCCAGTCCCGCGACAATCGCTCCCATCAc                                                                                                      <  2:921101/151‑1 (MQ=255)                                        aTCGCACGTTGTGAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTCTGGCTGACATACACACCAGCAATAATGCCAGTCCCGCGACAATCGCTCCCATCAcc                                                                                                     >  1:759444/1‑151 (MQ=255)                                            cACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTCTGGCTGACATACACACCAGCAATAATGCCAGTCCCGCGACAATCGCTCCCATCACCGGca                                                                                                 <  2:221127/151‑1 (MQ=255)                                            cACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTCTGGCTGACATACACACCAGCAATAATGCCAGTCCCGCGACAATCGCTCCCATCACCGGc                                                                                                  >  2:789790/1‑150 (MQ=255)                                             aCGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTCTGGCTGACATACACACCAGCAATAATGCCAGTCCCGCGACAATCGCTCCCATCACCGGcac                                                                                                >  1:426172/1‑151 (MQ=255)                                                 tGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTCTGGCTGACATACACACCAGCAATAATGCCAGTCCCGCGACAATCGCTCCCATCACCGGCACaaa                                                                                             <  1:68295/150‑1 (MQ=255)                                                      gCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTCTGGCTGACATACACACCAGCAATAATGCCAGTCCCGCGACAATCGCTCCCATCACCGGCACAAAGCTGt                                                                                        >  2:393201/1‑150 (MQ=255)                                                       cAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTCTGGCTGACATACACACCAGCAATAATGCCAGTCCCGCGACAATCGCTCCCATCACCGGCACAAAGCTGTAt                                                                                      >  2:506145/1‑151 (MQ=255)                                                          cTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTCTGGCTGACATACACACCAGCAATAATGCCAGTCCCGCGACAATCGCTCCCATCACCGGCACAAAGCTGTATccc                                                                                   >  2:448581/1‑151 (MQ=255)                                                            gTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTCTGGCTGACATACACACCAGCAATAATGCCAGTCCCGCGACAATCGCTCCCATCACCGGCACAAAGCTGTATcc                                                                                    <  1:224773/148‑1 (MQ=255)                                                                 gCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTCTGGCTGACATACACACCAGCAATAATGCCAGTCCCGCGACAATCGCTCCCATCACCGGCACAAAGCTGTATcccagccc                                                                              >  2:340685/1‑149 (MQ=255)                                                                      aGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTCTGGCTGACATACACACCAGCAATAATGCCAGTCCCGCGACAATCGCTCCCATCACCGGCACAAAGCTGTATCCCAGCCCAGCGGa                                                                        >  1:729517/1‑150 (MQ=255)                                                                        gTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTCTGGCTGACATACACACCAGCAATAATGCCAGTCCCGCGACAATCGCTCCCATCACCGGCACAAAGCTGTa                                                                                       >  1:882898/1‑133 (MQ=255)                                                                        gTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTCTGGCTGACATACACACCAGCAATAATGCCAGTCCCGCGACAATCGCTCCCATCACCGGCACAAAGCTGTa                                                                                       <  2:882918/133‑1 (MQ=255)                                                                        gTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTCTGGCTGACATACACACCAGCAATAATGCCAGTCCCGCGACAATCGCTCCCATCACCGGCACAAAGCTGTATCCCAGCCCAGCGGAAAt                                                                     >  2:676501/1‑151 (MQ=255)                                                                         ttATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTCTGGCTGACATACACACCAGCAATAATGCCAGTCCCGCGACAATCGCTCCCATCACCGGCACAAAGCTGTATCCCAGCCCAGCGGAAAtt                                                                    >  1:814342/1‑151 (MQ=255)                                                                          tATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTCTGGCTGACATACACACCAGCAATAATGCCAGTCCCGCGACAATCGCTCCCATCACCGGCACAAAGCTGTATCCCAGCCCAGCGGAAAtt                                                                    <  1:924980/150‑1 (MQ=255)                                                                          tATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTCTGGCTGACATACACACCAGCAATAATGCCAGTCCCGCGACAATCGCTCCCATCACCGGCACAAAGCTGTATCCCAGCCCAGCGGAAAtt                                                                    >  2:40615/1‑150 (MQ=255)                                                                                tCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTCTGGCTGACATACACACCAGCAATAATGCCAGTCCCGCGACAATCGCTCCCATCACCGGCACAAAGCTGTATCCCAGCCCAGCGGAAATTACCGca                                                              <  2:497409/150‑1 (MQ=255)                                                                                         ttGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTCTGGCTGACATACACACCAGCAATAATGCCAGTCCCGCGACAATCGCTCCCATCACCGGCACAAAGCTGTATCCCAGCCCAGCGGAAATTACCGCACCACcagca                                                     <  1:157183/150‑1 (MQ=255)                                                                                             ttAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTCTGGCTGACATACACACCAGCAATAATGCCAGTCCCGCGACAATCGCTCCCATCACCGGCACAAAGCTGTATCCCAGCCCAGCGGAAATTACCGCACCACCAGCAgctg                                                 >  2:606744/1‑150 (MQ=255)                                                                                               aaTTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTCTGGCTGACATACACACCAGCAATAATGCCAGTCCCGCGACAATCGCTCCCATCACCGGCACAAAGCTGTATCCCAGCCCAGCGGAAATTACCGCACCACCAGCAgctgct                                               <  1:332665/150‑1 (MQ=255)                                                                                                aTTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTCTGGCTGACATACACACCAGCAATAATGCCAGTCCCGCGACAATCGCTCCCATCACCGGCACAAAGCTGTATCCCAGCCCAGCGGAAATTACCGCACCACCAGCAGCTGCTcc                                             >  1:855582/1‑151 (MQ=255)                                                                                                aTTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTCTGGCTGACATACACACCAGCAATAATGCCAGTCCCGCGACAATCGCTCCCATCACCGGCACAAAGCTGTATCCCAGCCCAGCGGAAATTACCGCACCACCAGCAGCTGCTcc                                             <  1:399391/151‑1 (MQ=255)                                                                                                   aGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTCTGGCTGACATACACACCAGCAATAATGCCAGTCCCGCGACAATCGCTCCCATCACCGGCACAAAGCTGTATCCCAGCCCAGCGGAAATTACCGCACCACCAGCAGCTGCTccc                                            >  1:311004/1‑149 (MQ=255)                                                                                                      tGTTAGCAACGCAAACTGTTTCAGGTTGTTTTCTGGCTGACATACACACCAGCAATAATGCCAGTCCCGCGACAATCGCTCCCATCACCGGCACAAAGCTGTATCCCAGCCCAGCGGAAATTACCGCACCACCAGCAGCTGCTCCCa                                           <  2:600078/147‑1 (MQ=255)                                                                                                      tGTTAGCAACGCAAACTGTTTCAGGTTGTTTTCTGGCTGACATACACACCAGCAATAATGCCAGTCCCGCGACAATCGCTCCCATCACCGGCACAAAGCTGTATCCCAGCCCAGCGGAAATTACCGCACCACCAGCAGCTGCTCCCAgcgc                                       >  1:730663/1‑151 (MQ=255)                                                                                                       gTTAGCAACGCAAACTGTTTCAGGTTGTTTTCTGGCTGACATACACACCAGCAATAATGCCAGTCCCGCGACAATCGCTCCCATCACCGGCACAAAGCTGTATCCCAGCCCAGCGGAAATTACCGCACCACCAGCAGCTGCTCCCAgcg                                        <  2:792830/149‑1 (MQ=255)                                                                                                        ttAGCAACGCAAACTGTTTCAGGTTGTTTTCTGGCTGACATACACACCAGCAATAATGCCAGTCCCGCGACAATCGCTCCCATCACCGGCACAAAGCTGTATCCCAGCCCAGCGGAAATTACCGCACCACCAGCAGCTGCTCCCAGCGCAt                                     >  1:253297/1‑151 (MQ=255)                                                                                                          aGCAACGCAAACTGTTTCAGGTTGTTTTCTGGCTGACATACACACCAGCAATAATGCCAGTCCCGCGACAATCGCTCCCATCACCGGCACAAAGCTGTATCCCAGCCCAGCGGAAATTACCGCACCACCAGCAGCTGCTCCCAGCGCAttt                                   >  2:465449/1‑151 (MQ=255)                                                                                                                  aaaCTGTTTCAGGTTGTTTTCTGGCTGACATACACACCAGCAATAATGCCAGTCCCGCGACAATCGCTCCCATCACCGGCACAAAGCTGTATCCCAGCCCAGCGGAAATTACCGCACCACCAGCAGCTGCTCCCAGCGCATTTCCAAGAtt                           >  2:562786/1‑151 (MQ=255)                                                                                                                   aaCTGTTTCAGGTTGTTTTCTGGCTGACATACACACCAGCAATAATGCCAGTCCCGCGACAATCGCTCCCATCACCGGCACAAAGCTGTATCCCAGCCCAGCGGAAATTACCGCACCACCAGCAGCTGCTCCCAGCGCATTTCCAAGAtt                           >  1:713768/1‑150 (MQ=255)                                                                                                                      tGTTTCAGGTTGTTTTCTGGCTGACATACACACCAGCAATAATGCCAGTCCCGCGACAATCGCTCCCATCACCGGCACAAAGCTGTATCCCAGCCCAGCGGAAATTACCGCACCACCAGCAGCTGCTCCCAGCGCATTTCCAAGATTaa                         >  2:396594/1‑149 (MQ=255)                                                                                                                       gTTTCAGGTTGTTTTCTGGCTGACATACACACCAGCAATAATGCCAGTCCCGCGACAATCGCTCCCATCACCGGCACAAAGCTGTATCCCAGCCCAGCGGAAATTACCGCACCACCAGCAGCTGCTCCCAGCGCATTTCCAAGATTAAAgg                      >  2:309197/1‑151 (MQ=255)                                                                                                                       gTTTCAGGTTGTTTTCTGGCTGACATACACACCAGCAATAATGCCAGTCCCGCGACAATCGCTCCCATCACCGGCACAAAGCTGTATCCCAGCCCAGCGGAAATTACCGCACCACCAGCAGCTGCTCCCAGCGCATTTCCAAGATTAAAg                       <  2:439481/150‑1 (MQ=255)                                                                                                                                 gttTTCTGGCTGACATACACACCAGCAATAATGCCAGTCCCGCGACAATCGCTCCCATCACCGGCACAAAGCTGTATCCCAGCCCAGCGGAAATTACCGCACCACCAGCAGCTGCTCCCAGCGCATTTCCAAGATTAAAGGCACCAATAtt            <  1:902714/151‑1 (MQ=255)                                                                                                                                       tGGCTGACATACACACCAGCAATAATGCCAGTCCCGCGACAATCGCTCCCATCACCGGCACAAAGCTGTATCCCAGCCCAGCGGAAATTACCGCACCACCAGCAGCTGCTCCCAGCGCATTTCCAAGATTAAAGGCACCAATATTGACtg       >  1:31561/1‑150 (MQ=255)                                                                                                                                            gACATACACACCAGCAATAATGCCAGTCCCGCGACAATCGCTCCCATCACCGGCACAAAGCTGTATCCCAGCCCAGCGGAAATTACCGCACCACCAGCAGCTGCTCCCAGCGCATTTCCAAGATTAAAGGCACCAATATTGACTGATGaa  <  1:189391/150‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                                GTAGGTCGGATAAGGCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTCTGGCTGACATAAACACCAGCAATAATGCCAGTCCCGCGACAATCGCTCCCATCACCGGCACAAAGCTGTATCCCAGCCCAGCGGAAATTACCGCACCACCAGCAGCTGCTCCCAGCGCATTTCCAAGATTAAAGGCACCAATATTGACTGATGAA  >  NC_000913/1736025‑1736313

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑