Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,822,544	T→G	F29L (TTT→TTG)	nadE →	NAD synthetase, NH3/glutamine‑dependent

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,822,544	0	T	G	100.0%	238.7 / NA	62	F29L (TTT→TTG)	nadE	NAD synthetase, NH3/glutamine‑dependent
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (35/27);  total (35/27)

GTTTCAGTAGCAACGGGTTAGCTTTAAGGAAGTTTTGTCTTTTCTGTCTGGAGGGGTTCAATGACATTGCAACAACAAATAATAAAGGCGCTGGGCGCAAAACCGCAGATTAATGCTGAAGAGGAAATTCGTCGTAGTGTCGATTTTCTGAAAAGCTACCTGCAAACTTATCCGTTCATTAAATCACTGGTGCTCGGGATCAGCGGCGGTCAGGACTCCACGCTTGCCGGAAAGCTGTGCCAGATGGCGATTAATGAGCTGCGCCTGGAAACCGGCAACGAATCACTGCA  >  NC_000913/1822398‑1822687                                                                                                                                                   |                                                                                                                                                gTTTCAGTAGCAACGGGTTAGCTTTAAGGAAGTTTTGTCTTTTCTGTCTGGCGGGGTTCAATGACATTGCAACAACAAATAATAACGGCGCTGGGCGCAAAACCGCAGATTAATGCTGAAGAGGAAATTCGTCGTAGTGTCGATTTGCTg                                                                                                                                              >  2:199749/1‑150 (MQ=255)     cAGTAGCAACGGGTTAGCTTTAAGGAAGTTTTGTCTTTTCTGTCTGGAGGGGTTCAATGACATTGCAACAACAAATAATAAAGGCGCTGGGCGCAAAACCGCAGATTAATGCTGAAGAGGAAATTCGTCGTAGTGTCGATTTGCTGaaa                                                                                                                                           >  2:77128/1‑149 (MQ=255)      aggaGCAACGGGTTAGCTTTAAGGAAGTTTTGTCTTTTCTGTCTGGAGGGGTTCAATGACATTGCAACAACAAATAATAAAGGCGCTGGGCGCAAAACCGCAGATTAATGCTGAAGAGGAAATTCGTCGTAGTGTCGATTTGCTGaaaa                                                                                                                                          <  1:287036/146‑1 (MQ=255)      aGTAGCAACGGGTTAGCTTTAAGGAAGTTTTGTCTTTTCTGTCTGGAGGGGTTCAATGACATTGCAACAACAAATAATAAAGGCGCTGGGCGCAAAACCGCAGATTAATGCTGAAGAGGAAATTCGTCGTAGTGTCGATTTGCTGAAAAGc                                                                                                                                        >  2:306447/1‑151 (MQ=255)       gTAGCAACGGGTTAGCTTTAAGGAAGTTTTGTCTTTTCTGTCTGGAGGGGTTCAATGACATTGCAACAACAAATAATAAAGGCGCTGGGCGCAAAACCGCAGATTAATGCTGAAGAGGAAATTCGTCGTAGTGTCGATTTGCTGAAAAGCt                                                                                                                                       <  1:378759/151‑1 (MQ=255)            aaCGGGTTAGCTTTAAGGAAGTTTTGTCTTTTCTGTCTGGAGGGGTTCAATGACATTGCAACAACAAATAATAAAGGCGCTGGGCGCAAAACCGCAGATTAATGCTGAAGAGGAAATTCGTCGTAGTGTCGATTTGCTGAAAAGCTACCTg                                                                                                                                  <  1:510886/151‑1 (MQ=255)              cGGGTTAGCTTTAAGGAAGTTTTGTCTTTTCTGTCTGGAGGGGTTCAATGACATTGCAACAACAAATAATAAAGGCGCTGGGCGCAAAACCGCAGATTAATGCTGAAGAGGAAATTCGTCGTAGTGTCGATTTGCTGAAAAGCTACCTGc                                                                                                                                 >  2:349615/1‑150 (MQ=255)                ggTTAGCTTTAAGGAAGTTTTGTCTTTTCTGTCTGGAGGGGTTCAATGACATTGCAACAACAAATAATAAAGGCGCTGGGCGCAAAACCGCAGATTAATGCTGAAGAGGAAATTCGTCGTAGTGTCGATTTGCTGAAAAGCTACCTGCaaa                                                                                                                              <  1:698449/151‑1 (MQ=255)                    aGCTTTAAGGAAGTTTTGTCTTTTCTGTCTGGAGGGGTTCAATGACATTGCAACAACAAATAATAAAGGCGCTGGGCGCAAAACCGCAGATTAATGCTGAAGAGGAAATTCGTCGTAGTGTCGATTTGCTGAAAAGCTACCTGCAAACtt                                                                                                                           <  1:227749/150‑1 (MQ=255)                     gCTTTAAGGAAGTTTTGTCTTTTCTGTCTGGAGGGGTTCAATGACATTGCAACAACAAATAATAAAGGCGCTGGGCGCAAAACCGCAGATTAATGCTGAAGAGGAAATTCGTCGTAGTGTCGATTTGCTGAAAAGCTACCTGCAAACTTAt                                                                                                                         >  1:384789/1‑151 (MQ=255)                       tttAAGGAAGTTTTGTCTTTTCTGTCTGGAGGGGTTCAATGACATTGCAACAACAAATAATAAAGGCGCTGGGCGCAAAACCGCAGATTAATGCTGAAGAGGAAATTCGTCGTAGTGTCGATTTGCTGAAAAGCTACCTGCAAACTTATcc                                                                                                                       >  1:368533/1‑151 (MQ=255)                        ttAAGGAAGTTTTGTCTTTTCTGTCTGGAGGGGTTCAATGACATTGCAACAACAAATAATAAAGGCGCTGGGCGCAAAACCGCAGATTAATGCTGAAGAGGAAATTCGTCGTAGTGTCGATTTGCTGAAAAGCTACCTGCAAACTTATCCg                                                                                                                      >  2:443559/1‑151 (MQ=255)                          aaGGAAGTTTTGTCTTTTCTGTCTGGAGGGGTTCAATGACATTGCAACAACAAATAATAAAGGCGCTGGGCGCAAAACCGCAGATTAATGCTGAAGAGGAAATTCGTCGTAGTGTCGATTTGCTGAAAAGCTACCTGCAAACTTATCCGtt                                                                                                                    >  2:208211/1‑151 (MQ=255)                              gaGTTTTGTCTTTTCTGTCTGGAGGGGTTCAATGACATTGCAACAACAAATAATAAAGGCGCTGGGCGCAAAACCGCAGATTAATGCTGAAGAGGAAATTCGTCGTAGTGTCGATTTGCTGAAAAGCTACCTGCAAACTTATCCGTTCAtt                                                                                                                >  1:12044/2‑151 (MQ=255)                              aaGTTTTGTCTTTTCTGTCTGGAGGGGTTCAATGACATTGCAACAACAAATAATAAAGGCGCTGGGCGCAAAACCGCAGATTAATGCTGAAGAGGAAATTCGTCGTAGTGTCGATTTGCTGAAAAGCTACCTGCAAACTTATCCGTTCAtt                                                                                                                <  2:794956/151‑1 (MQ=255)                               aGTTTTGTCTTTTCTGTCTGGAGGGGTTCAATGACATTGCAACAACAAATAATAAAGGCGCTGGGCGCAAAACCGCAGATTAATGCTGAAGAGGAAATTCGTCGTAGTGTCGATTTGCTGAAAAGCTACCTGCAAACTTATCCGTTCATTa                                                                                                               <  1:780895/151‑1 (MQ=255)                                    tGTCTTTTCTGTCTGGAGGGGTTCAATGACATTGCAACAACAAATAATAAAGGCGCTGGGCGCAAAACCGCAGATTAATGCTGAAGAGGAAATTCGTCGTAGTGTCGATTTGCTGAAAAGCTACCTGCAAACTTATCCGTTCATTAAATc                                                                                                           <  1:447604/150‑1 (MQ=255)                                    tGTCTTTTCTGTCTGGAGGGGTTCAATGACATTGCAACAACAAATAATAAAGGCGCTGGGCGCAAAACCGCAGATTAATGCTGAAGAGGAAATTCGTCGTAGTGTCGATTTGCTGAAAAGCTACCTGCAAACTTATCCGTTCATTAAATc                                                                                                           <  2:472841/150‑1 (MQ=255)                                     gTCTTTTCTGTCTGGAGGGGTTCAATGACATTGCAACAACAAATAATAAAGGCGCTGGGCGCAAAACCGCAGATTAATACTGAAGAGGAAATTCGTCGTAGTGTCGATTTGCTGAAAAGCTACCTGCAAACTTATCCGTTCATTAAATCa                                                                                                          <  2:317580/150‑1 (MQ=255)                                       cTTTTCTGTCTGGAGGGGATCAATGACATTGCAACAACAAATAATAAAGGCGCTGGGCGCAAAACCGCAGATTAATGCTGAAGAGGAAATTCGTCGTAGTGTCGATTTGCTGAAAAGCTAACTGCAAACTTATCCGTTCATTAAATCACTg                                                                                                       >  1:347871/1‑151 (MQ=255)                                         tttCTGTCTGGAGGGGTTCAATGACATTGCAACAACAAATAATAAAGGCGCTGGGCGCAAAACCGCAGATTAATGCTGAAGAGGAAATTCGTCGTAGTGTCGATTTGCTGAAAAGCTACCTGCAAACTTATCCGTTCATTAAATCACt                                                                                                        <  2:770044/148‑1 (MQ=255)                                         tttCTGTCTGGAGGGGTTCAATGACATTGCAACAACAAATAATAAAGGCGCTGGGCGCAAAACCGCAGATTAATGCTGAAGAGGAAATTCGTCGTAGTGTCGATTTGCTGAAAAGCTACCTGCAAACTTATCCGTTCATTAAATCACTgg                                                                                                      <  1:54071/150‑1 (MQ=255)                                           tctgtctgGAGGGGTTCAATGACATTGCAACAACAAATAATAAAGGCGCTGGGCGCAAAACCGCAGATTAATGCTGAAGAGGAAATTCGTCGTAGTGTCGATTTGCTGAAAAGCTACCTGCAAACTTATCCGTTCATTAAATCACTGGTg                                                                                                    <  1:921705/150‑1 (MQ=255)                                            ctgtctgGAGGGGTTCAATGACATTGCAACAACAAATAATAAAGGCGCTGGGCGCAAAACCGCAGATTAATGCTGAAGAGGAAATTCGTCGTAGTGTCGATTTGCTGAAAAGCTACCTGCAAACTTATCCGTTCATTAAATCACTGGTGCt                                                                                                  >  2:30425/1‑151 (MQ=255)                                            ctgtctgGAGGGGTTCAATGACATTGCAACAACAAATAATAAAGGCGCTGGGCGCAAAACCGCAGATTAATGCTGAAGAGGAAATTCGTCGTAGTGTCGATTTGCTGAAAAGCTACCTGCAAACTTATCCGTTCATTAAATCACTGGTGCt                                                                                                  >  1:922233/1‑151 (MQ=255)                                              gtctgGAGGGGTTCAATGACATTGCAACAACAAATAATAAAGGCGCTGGGCGCAAAACCGCAGATTAATGCTGAAGAGGAAATTCGTCGTAGTGTCGATTTGCTGAAAAGCTACCTGCAAACTTATCCGTTCATTAAATCACTGGTGCTCg                                                                                                >  1:749232/1‑151 (MQ=255)                                                   gAGGGGTTCAATGACATTGCAACAACAAATAATAAAGGCGCTGGGCGCAAAACCGCAGATTAATGCTGAAGAGGAAATTCGTCGTAGTGTCGATTTGCTGAAAAGCTACCTGCAAACTTATCCGTTCATTAAATCACTGGTGCTCGGGATc                                                                                           >  2:562400/1‑151 (MQ=255)                                                        gTTCAATGACATTGCAACAACAAATAATAAAGGCGCTGGGCGCAAAACCGCAGATTAATGCTGAAGAGGAAATTCGTCGTAGTGTCGATTTGCTGAAAAGCTACCTGCAAACTTATCCGTTCATTAAATCACTGGTGCTCGGGATCAgcgg                                                                                      >  1:48805/1‑151 (MQ=255)                                                          tCAATGACATTGCAACAACAAATAATAAAGGCGCTGGGCGCAAAACCGCAGATTAATGCTGAAGAGGAAATTCGTCGTAGTGTCGATTTGCTGAAAAGCTACCTGCAAACTTATCCGTTCATTAAATCACTGGTGCTCGGGATCAgcggc                                                                                     <  1:311152/150‑1 (MQ=255)                                                             aTGACATTGCAACAACAAATAATAAAGGCGCTGGGCGCAAAACCGCAGATTAATGCTGAAGAGGAAATTCGTCGTAGTGTCGATTTGCTGAAAAGCTACCTGCAAACTTATCCGTTCATTAAATCACTGGTGCTCGGGATCAGCGGCGGTc                                                                                 <  2:631893/151‑1 (MQ=255)                                                                 cATTGCAACAACAAATAATAAAGGCGCTGGGCGCAAAACCGCAGATTAATGCTGAAGAGGAAATTCGTCGTAGTGTCGATTTGCTGAAAAGCTACCTGCAAACTTATCCGTTCATTAAATCACTGGTGCTCGGGATCAGCGGCGGTCAgg                                                                              >  2:143139/1‑150 (MQ=255)                                                                   ttGGAACAAAAAATAATAAAGGCGCTGGGCGCAAAACCGCAGATTAATGCTGAAGAGGAAATTCGTCGTAGTGTCGATTTGCTGAAAAGCTACCTGCAAACTTATCCGATCATTAAATCACTGGTGCTCGGGATAAGCGGCGGCCa                                                                                >  1:751794/1‑146 (MQ=255)                                                                       aacaacaaATAATAAAGGCGCTGGGCGCAAAACCGCAGATTAATGCTGAAGAGGAAATTCGTCGTAGTGTCGATTTGCTGAAAAGCTACCTGCAAACTTATCCGTTCATTAAATCACTGGTGCTCGGGATCAGCGGCGGTCAGGACTCCAc                                                                       <  1:425473/151‑1 (MQ=255)                                                                            caaATAATAAAGGCGCTGGGCGCAAAACCGCAGATTAATGCTGAAGAGGAAATTCGTCGTAGTGTCGATTTGCTGAAAAGCTACCTGCAAACTTATCCGTTCATTAAATCACTGGTGCTCGGGATCAGCGGCGGTCAGGACTCCACGCtt                                                                   >  2:105817/1‑150 (MQ=255)                                                                             aaaTAATAAAGGCGCTGGGCGCAAAACCGCAGATTAATGCTGAAGAGGAAATTCGTCGTAGTGTCGATTTGCTGAAAAGCTACCTGCAAACTTATCCGTTCATTAAATCACTGGTGCTCGGGATCAGCGGAGGTCAGGACTCCACGCTTGc                                                                 >  2:542049/1‑151 (MQ=255)                                                                              aataatAAAGGCGCTGGGCGCAAAACCGCAGATTAATGCTGAAGAGGAAATTCGTCGTAGTGTCGATTTGCTGAAAAGCTACCTGCAAACTTATCCGTTCATTAAATCACTGGTGCTCGGGATCAGCGGCGGTAAGGTCTCCACGCTTGcc                                                                >  1:134441/1‑151 (MQ=255)                                                                                   taaAGGCGCTGGGCGCAAAACCGCAGATTAATGCTGAAGAGGAAATTCGTCGTAGTGTCGATTTGCTGAAAAGCTACCTGCAAACTTATCCGTTCATTAAATCACTGGTGCTCGGGATCAGCGGCGGTCAGGACTCCACGCTTGCCGGaa                                                            >  1:128662/1‑150 (MQ=255)                                                                                       ggCGCTGGGCGCAAAACCGCAGATTAATGCTGAAGAGGAAATTCGTCGTAGTGTCGATTTGCTGAAAAGCTACCTGCAAACTTATCCGTTCATTAAATCACTGGTGCTCGGGATCAGCGGCGGTCAGGACTCCACGCTTGCCGGAAAGCtg                                                       <  2:739515/151‑1 (MQ=255)                                                                                         cgcTGGGCGCAAAACCGCAGATTAATGCTGAAGAGGAAATTCGTCGTAGTGTCGATTTGCTGAAAAGCTACCTGCAAACTTATCCGTTCATTAAATCACTGGTGCTCGGGATCAGCGGCGGTCAGGACTCCACGCTTGCCGGAAAGCtgtg                                                     >  2:916340/1‑151 (MQ=255)                                                                                           cTGGGCGCAAAACCGCAGATTAATGCTGAAGAGGAAATTCGTCGTAGTGTCGATTTGCTGAAAAGCTACCTGCAAACTTATCCGTTCATTAAATCACTGGTGCTCGGGATCAGCGGCGGTCAGGACTCCACGCTTGCCGGAAAGCTGTGcc                                                   >  2:436977/1‑151 (MQ=255)                                                                                                 gcAAAACCGCAGATTAATGCTGAAGAGGAAATTCGTCGTAGTGTCGATTTGCTGAAAAGCTGCCTGCAAACTTATCCGTTCATTAAATCACTGGTGCTCGGGATCAGCGGCGGTCAGGACTCCACGCTTGCCGGAAAGCTGTGCCAGATgg                                             <  2:459246/151‑1 (MQ=255)                                                                                                 gcAAAACCGCAGATTAATGCTGAAGAGGAAATTCGTCGTAGTGTCGATTTGCTGAAAAGCTACCTGCAAACTTATCCGTTCATTAAATCACTGGTGCTCGGGATCAGCGGCGGTCAGGACTCCACGCTTGCCGGAAAGCTGTGCCAGATgg                                             >  1:18290/1‑151 (MQ=255)                                                                                                  cAAAACCGCAGATTAATGCTGAAGAGGAAATTCGTCGTAGTGTCGATTTGCTGAAAAGCTACCTGCAAACTTATCCGTTCATTAAATCACTGGTGCTCGGGATCAGCGGCGGTCAGGACTCCACGCTTGCCGGAAAGCTGTGCCAGATgg                                             >  1:723450/1‑150 (MQ=255)                                                                                                  cAAAACCGCAGATTAATGCTGAAGAGGAAATTCGTCGTAGTGTCGATTTGCTGAAAAGCTACCTGCAAACTTATCCGTTCATTAAATCACTGGTGCTCGGGATCAGCGGCGGTCAGGACTCCACGCTTGCCGGAAAGCTGTGCCAGATGGc                                            <  2:784950/151‑1 (MQ=255)                                                                                                      aCCGCAGATTAATGCTGAAGAGGAAATTCGTCGTAGTGTCGATTTGCTGAAAAGCTACCTGCAAACTTATCCGTTCATTAAATCACTGGTGCTCGGGATCAGCGGCGGTCAGGACTCCACGCTTGCCGGAAAGCTGTGCCAGATGGCGAtt                                        <  1:921421/151‑1 (MQ=255)                                                                                                            gATTAATGCTGAAGAGGAAATTCGTCGTAGTGTCGATTTGCTGAAAAGCTACCTGCAAACTTATCCGTTCATTAAATCACTGGTGCTCGGGATCAGCGGCGGTCAGGACCCCACGCTTGCCGGAAAGCTGTGCCAGAGGGCGAATAATgtg                                  >  1:56514/1‑149 (MQ=255)                                                                                                             aTTAATGCTGAAGAGGAAATTCGTCGTAGTGTCGATTTGCTGAAAAGCTACCTGCAAACTTATCCGTTCATTAAATCACTGGTGCTCGGGATCAGCGGCGGTCAGGACTCCACGCTTGCCGGAAAGCTGTGCCAGATGGCGATTAATGAGc                                 >  2:557117/1‑151 (MQ=255)                                                                                                              ttAATGCTGAAGAGGAAATTCGTCGTAGTGTCGATTTGCTGAAAAGCTACCTGCAAACTTATCCGTTCATTAAATCACTGGTGCTCGGGATCAGCGGCGGTCAGGACTCCACGCTTGCCGGAAAGCTGTGCCAGATGGCGATTAATGAGCt                                >  1:692162/1‑151 (MQ=255)                                                                                                                aaTGCTGAAGAGGAAATTCGTCGTAGTGTCGATTTGCTGAAAAGCTACCTGCAAACTTATCCGTTCATTAAATCACTGGTGCTCGGGATCAGCGGCGGTCAGGACTCCACGCTTGCCGGAAAGCTGTGCCAGATGGCGATTAATGAGCTg                               <  2:86321/150‑1 (MQ=255)                                                                                                                    cTGAAGAGGAAATTCGTCGTAGTGTCGATTTGCTGAAAAGCTACCTGCAAACTTATCCGTTCATTAAATCACTGGTGCTCGGGATCAGCGGCGGTCAGGACTCCACGCTTGCCGGAAAGCTGTGCCAGATGGCGATTAATGAGCTGCGcc                           >  2:529919/1‑150 (MQ=255)                                                                                                                     tGAAGAGGAAATTCGTCGTAGTGTCGATTTGCTGAAAAGCTACCTGCAAACTTATCCGTTCATTAAATCACTGGTGCTCGGGATCAGCGGCGGTCAGGACTCCACGCTTGCCGGAAAGCTGTGCCAGATGGCGATTAATGAGCTGCGCCTg                         >  2:793806/1‑151 (MQ=255)                                                                                                                          agGAAATTCGTCGTAGTGTCGATTTGCTGAAAAGCTACCTGCAAACTTATCCGTTCATTAAATCACTGGTGCTCGGGATCAGCGGCGGTCAGGACTCCACGCTTGCCGGAAAGCTGTGCCAGATGGCGATTAATGAGCTGCGCCTGGAAAc                    <  1:578773/151‑1 (MQ=255)                                                                                                                            gAAATTCGTCGTAGTGTCGATTTGCTGAAAAGCTACCTGCAAACTTATCCGTTCATTAAATCACTGGTGCTCGGGATCAGCGGCGGTCAGGACTCCACGCTTGCCGGAAAGCTGTGCCAGATGGCGATTAATGAGCTGCGCCTGGAAACCg                  <  1:49911/151‑1 (MQ=255)                                                                                                                              aaTTCGTCGTAGTGTCGATTTGCTGAAAAGCTACCTGCAAACTTATCCGTTCATTAAATCACTGGTGCTCGGGATCAGCGGCGGTCAGGACTCCACGCTTGCCGGAAAGCTGTGCCAGATGGCGATTAATGAGCTGCGCCTGGAAACCGGc                <  1:332982/151‑1 (MQ=255)                                                                                                                                ttCGTCGTAGTGTCGATTTGCTGAAAAGCTACCTGCAAACTTATCCGTTCATTAAATCACTGGTGCTCGGGATCAGCGGCGGTCAGGACTCCACGCTTGCCGGAAAGCTGTGCCAGATGGCGATTAATGAGCTGCGCCTGGAAACCGGCa               >  1:242157/1‑150 (MQ=255)                                                                                                                                  cgtcgtAGTGTCGATTTGCTGAAAAGCTACCTGCAAACTTATCCGTTCATTAAATCACTGGTGCTCGGGATCAGCGGCGGTCAGGACTCCACGCTTGCCGGAAAGCTGTGCCAGATGGCGATTAATGAGCTGCGCCTGGAAACCGGCAAc             <  2:267633/150‑1 (MQ=255)                                                                                                                                    tcgtAGTGTCGATCTGCTGAAAAGCTACCTGCAAACTTATCCGTTCATTAAATCACTGGTGCTCGGGATCAGCGGCGGTCAGGACTCCACGCTTGCCGGAAAGCTGTGCCAGATGGCGATTAATGAGCTGCGCCTGGAAACCGGCAACGa           >  1:418666/1‑150 (MQ=255)                                                                                                                                       tAGTGTCGATTTGCTGAAAAGCTACCTGCAAACTTATCCGTTCATTAAATCACTGGTGCTCGGGATCAGCGGCGGTCAGGACTCCACGCTTGCCGGAAAGCTGTGCCAGATGGCGATTAATGAGCTGCGCCTGGAAACCGGCAACGAATc        >  2:2966/1‑150 (MQ=255)                                                                                                                                        aGTGTCGATTTGCTGAAAAGCTACCTGCAAACTTATCCGTTCATTAAATCACTGGTGCTCGGGATCAGCGGCGGTCAGGACTCCACGCTTGCCGGAAAGCTGTGCCAGATGGCGATTAATGAGCTGCGCCTGGAAACCGGCAACGAATCa       <  1:182628/150‑1 (MQ=255)                                                                                                                                        aGTGTCGATTTGCTGAAAAGCTACCTGCAAACTTATCCGTTCATTAAATCACTGGTGCTCGGGATCAGCGGCGGTCAGGACTCCACGCTTGCCGGAAAGCTGTGCCAGATGGCGATTAATGAGCTGCGCCTGGAAACCGGCAACGAATCAc      >  2:74812/1‑151 (MQ=255)                                                                                                                                             cGATTTGCTGAAAAGCTACCTGCAAACTTATCCGTTCATTAAATCACTGGTGCTCGGGATCAGCGGCGGTCAGGACTCCACGCTTGCCGGAAAGCTGTGCCAGATGGCGATTAATGAGCTGCGCCTGGAAACCGGCAACGAATCACTGCa  <  1:30423/150‑1 (MQ=255)                                                                                                                                             cGATTTGCTGAAAAGCTACCTGCAAACTTATCCGTTCATTAAATCACTGGTGCTCGGGATCAGCGGCGGTCAGGACTCCACGCTTGCCGGAAAGCTGTGCCAGATGGCGATTAATGAGCTGCGCCTGGAAACCGGCAACGAATCACTGCa  >  1:857841/1‑150 (MQ=255)                                                                                                                                                   |                                                                                                                                                GTTTCAGTAGCAACGGGTTAGCTTTAAGGAAGTTTTGTCTTTTCTGTCTGGAGGGGTTCAATGACATTGCAACAACAAATAATAAAGGCGCTGGGCGCAAAACCGCAGATTAATGCTGAAGAGGAAATTCGTCGTAGTGTCGATTTTCTGAAAAGCTACCTGCAAACTTATCCGTTCATTAAATCACTGGTGCTCGGGATCAGCGGCGGTCAGGACTCCACGCTTGCCGGAAAGCTGTGCCAGATGGCGATTAATGAGCTGCGCCTGGAAACCGGCAACGAATCACTGCA  >  NC_000913/1822398‑1822687

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑