Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,837,673	A→C	V154V (GTA→GTC)	ynjC →	putative ABC transporter permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,837,673	0	A	C	100.0%	193.3 / NA	52	V154V (GTA→GTC)	ynjC	putative ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (36/16);  total (36/16)

CAGCGCCCTTCTGCTCTTTGCTGACGGAGGGCTGCTTTATGACTATTTCCCGTATTTCACTCCGCCAATGGACCGATTTGGCATCGGTCTGGGCCTCACCCTTGCAGTGAAAGAAAGCGCATTTCTGCTGTGGATCTTAGCAGCAGTATTGAGCGAAAAATGGCTGTTGCAGCAGGTCATTGTGCTGGATTCGCTGGGCTACAGCCGCTGGCAATGCCTGAACTGGCTGCTGTTGCCCTCCGTCGCGCCTGCGCTGGCAATGGCGATGCTGGCGATTGTTGCCTGGTCGCTGTC  >  NC_000913/1837526‑1837819                                                                                                                                                    |                                                                                                                                                   caGCGCCCTTCTGCTCTTTGCTGACGGAGGGCTGCTTTATGACTATTTCCCGTATTTCACTCCGCCAATGGACCGATTTGGCATCGGTCTGGGCCTCACCCTTGCAGTGAAAGAAAGCGCATTTCTGCTGTGGATCTTAGCAGCAGTCTTg                                                                                                                                                 >  2:898070/1‑151 (MQ=255)        cTTCTGCTCTTTGCTGACGGAGGGCTGCTTTATGACTATTTCCCGTATTTCACTCCGCCAATGGACCGATTTGGCATCGGTCTGGGCCTCACCCTTGCAGTGAAAGAAAGCGCATTTCTGCTGTGGATCTTAGCAGCAGTCTTGAGCGaa                                                                                                                                           >  2:704254/1‑150 (MQ=255)         ttCTGCTCTTTGCTGACGGAGGGCTGCTTTATGACTATTTCCCGTATTTCACTCCGCCAATGGACCGATTTGGCATCGGTCTGGGCCTCACCCTTGCAGTGAAAGAAAGCGCATTTCTGCTGTGGATCTTAGCAGCAGTCTTGAGCGaaa                                                                                                                                          >  2:417967/1‑150 (MQ=255)          tCTGCTCTTTGCTGACGGAGGGCTGCTTTATGACTATTTCCCGTATTTCACTCCGCCAATGGACCGATTTGGCATCGGTCTGGGCCTCACCCTTGCAGTGAAAGAAAGCGCATTTCTGCTGTGGATCTTAGCAGCAGTCTTGAGCGaaaa                                                                                                                                         >  1:409816/1‑150 (MQ=255)             gCTCTTTGCTGACGGAGGGCTGCTTTATGACTATTTCCCGTATTTCACTCCGCCAATGGACCGATTTGGCATCGGTCTGGGCCTCACCCTTGCAGTGAAAGAAAGCGCATTTCTGCTGTGGATCTTAGCAGCAGTCTTGAGCGAAAAATg                                                                                                                                      <  1:738976/150‑1 (MQ=255)              ctctTTGCTGACGGAGGGCTGCTTTATGACTATTTCCCGTATTTCACTCCGCCAATGGACCGATTTGGCATCGGTCTGGGCCTCACCCTTGCAGTGAAAGAAAGCGCATTTCTGCTGTGGATCTTAGCAGCAGTCTTGAGCGAAAAATgg                                                                                                                                     >  2:867786/1‑150 (MQ=255)                ctTTGCTGACGGAGGGCTGCTTTATGACTATTTCCCGTATTTCACTCCGCCAATGGACCGATTTGGCATCGGTCTGGGCCTCACCCTTGCAGTGAAAGAAAGCGCATTTCTGCTGTGGATCTTAGCAGCAGTCTTGAGCGAAAAATGGCTg                                                                                                                                  >  2:409144/1‑151 (MQ=255)                   tGCTGACGGAGGGCTGCTTTATGACTATTTCCCGTATTTCACTCCGCCAATGGACCGATTTGGCATCGGTCTGGGCCTCACCCTTGCAGTGAAAGAAAGCGCATTTCTGCTGTGGATCTTAGCAGCAGTCTTGAGCGAAAAATGGCTGtt                                                                                                                                <  2:671641/150‑1 (MQ=255)                    gCTGACGGAGGGCTGCTTTATGACTATTTCCCGTATTTCACTCCGCCAATGGACCGATTTGGCATCGGTCTGGGCCTCACCCTTGCAGTGAAAGAAAGCGCATTTCTGCTGTGGATCTTAGCAGCAGTCTTGAGCGAAAAATGGCTGTTgc                                                                                                                              >  1:921373/1‑151 (MQ=255)                    gCTGACGGAGGGCTGCTTTATGACTATTTCCCGTATTTCACTCCGCCAATGGACCGATTTGGCATCGGTCTGGGCCTCACCCTTGCAGTGAAAGAAAGCGCATTTCTGCTGTGGATCTTAGCAGCAGTCTTGAGCGAAAAATGGCTGTTgc                                                                                                                              <  1:767633/151‑1 (MQ=255)                       gACGGAGGGCTGCTTTATGACTATTTCCCGTATTTCACTCCGCCAATGGACCGATTTGGCATCGGTCTGGGCCTCACCCTTGCAGTGAAAGAAAGCGCATTTCTGCTGTGGATCTTAGCAGCAGTCTTGAGCGAAAAATGGCTGTTgcagc                                                                                                                           >  2:692477/1‑151 (MQ=255)                       gACGGAGGGCTGCTTTATGACTATTTCCCGTATTTCACTCCGCCAATGGACCGATTTGGCATCGGTCTGGGCCTCACCCTTGCAGTGAAAGAAAGCGCATTTCTGCTGTGGATCTTAGCAGCAGTCTTGAGCGAAAAATGGCTGTTgca                                                                                                                             <  2:811058/149‑1 (MQ=255)                       gACGGAGGGCTGCTTTATGACTATTTCCCGTATTTCACTCCGCCAATGGACCGATTTGGCATCGGTCTGGGCCTCACCCTTGCAGTGAAAGAAAGCGCATTTCTGCTGTGGATCTTAGCAGCAGTCTTGAGCGAAAAATGGCTGTTgca                                                                                                                             <  1:645094/149‑1 (MQ=255)                        aCGGAGGGCTGCTTTATGACTATTTCCCGTATTTCACTCCGCCAATGGACCGATTTGGCATCGGTCTGGGCCTCACCCTTGCAGTGAAAGAAAGCGCATTTCTGCTGTGGATCTTAGCAGCAGTCTTGAGCGAAAAATGGCTGTTgcagca                                                                                                                          <  2:727957/151‑1 (MQ=255)                         cGGAGGGCTGCTTTATGACTATTTCCCGTATTTCACTCCGCCAATGGACCGATTTGGCATCGGTCTGGGCCTCACCCTTGCAGTGAAAGAAAGCGCATTTCTGCTGTGGATCTTAGCAGCAGTCTTGAGCGAAAAATGGCTGTTgcagc                                                                                                                           >  2:726688/1‑149 (MQ=255)                          ggAGGGCTGCTTTATGACTATTTCCCGTATTTCACTCCGCCAATGGACCGATTTGGCATCGGTCTGGGCCTCACCCTTGCAGTGAAAGAAAGCGCATTTCTGCTGTGGATCTTAGCAGCAGTCTTGAGCGAAAAATGGCTGTTGCAGCAgg                                                                                                                        >  2:912787/1‑151 (MQ=255)                          ggAGGGCTGCTTTATGACTATTTCCCGTATTTCACTCCGCCAATGGACCGATTTGGCATCGGTCTGGGCCTCACCCTTGCAGTGAAAGAAAGCGCATTTCTGCTGTGGATCTTAGCAGCAGTCTTGAGCGAAAAATGGCTGTTGCAGCAgg                                                                                                                        >  2:523473/1‑151 (MQ=255)                            aGGGCTGCTTTATGACTATTTCCCGTATTTCACTCCGCCAATGGACCGATTTGGCATCGGTCTGGGCCTCACCCTTGCAGTGAAAGAAAGCGCATTTCTGCTGTGGATCTTAGCAGCAGTCTTGAGCGAAAAATGGCTGTTGCAGCAGGTc                                                                                                                      <  2:213940/151‑1 (MQ=255)                            aGGGCTGCTTTATGACTATTTCCCGTATTTCACTCCGCCAATGGACCGATTTGGCATCGGTCTGGGCCTCACCCTTGCAGTGAAAGAAAGCGCATTTCTGCTGTGGATCTTAGCAGCAGTCTTGAGCGAAAAATGGCTGTTGCAGCAGGTc                                                                                                                      <  1:464297/151‑1 (MQ=255)                                   ctTTATGACTATTTCCCGTATTTCACTCCGCCAATGGACCGATTTGGCATCGGTCTGGGCCTCACCCTTGCAGTGAAAGAAAGCGCATTTCTGCTGTGGATCTTAGCAGCAGTCTTGAGCGAAAAATGGCTGTTGCAGCAGGTCATTGTGc                                                                                                               >  1:365001/1‑151 (MQ=255)                                    tttATGACTATTTCCCGTATTTCACTCCGCCAATGGACCGATTTGGCATCGGTCTGGGCCTCACCCTTGCAGTGAAAGAAAGCGCATTTCTGCTGTGGATCTTAGCAGCAGTCTTGAGCGAAAAATGGCTGTTGCAGCAGGTCATTGTGCt                                                                                                              >  2:483630/1‑151 (MQ=255)                                     ttATGACTATTTCCCGTATTTCACTCCGCCAATGGACCGATTTGGCATCGGTCTGGGCCTCACCCTTGCAGTGAAAGAAAGCGCATTTCTGCTGTGGATCTTAGCAGCAGTCTTGAGCGAAAAATGGCTGTTGCAGCAGGTCATTGTGCTg                                                                                                             >  1:868097/1‑151 (MQ=255)                                       aTGACTATTTCCCGTATTTCACTCCGCCAATGGACCGATTTGGCATCGGTCTGGGCCTCACCCTTGCAGTGAAAGAAAGCGCATTTCTGCTGTGGATCTTAGCAGCAGTCTTGAGCGAAAAATGGCTGTTGCAGCAGGTCATTGTGCTgg                                                                                                            >  2:314806/1‑150 (MQ=255)                                            tATTTCCCGTATTTCACTCCGCCAATGGACCGATTTGGCATCGGTCTGGGCCTCACCCTTGCAGTGAAAGAAAGCGCATTTCTGCTGTGGATCTTAGCAGCAGTCTTGAGCGAAAAATGGCTGTTGCAGCAGGTCATTGTGCTGGATTCGc                                                                                                      >  1:480694/1‑151 (MQ=255)                                                 cccGTATTTCACTCCGCCAATGGACCGATTTGGCATCGGTCTGGGCCTCACCCTTGCAGTGAAAGAAAGCGCATTTCTGCTGTGGATCTTAGCAGCAGTCTTGAGCGAAAAATGGCTGTTGCAGCAGGTCATTGTGCTGGATTCGCTg                                                                                                    <  1:295382/148‑1 (MQ=255)                                                     tATTTCACTCCGCCAATGGACCGATTTGGCATCGGTCTGGGCCTCACCCTTGCAGTGAAAGAAAGCGCATTTCTGCTGTGGATCTTAGCAGCAGTCTTGAGCGAAAAATGGCTGTTGCAGCAGGTCATTGTGCTGGATTCGCTGGGCTAc                                                                                              >  2:575045/1‑150 (MQ=255)                                                             tCCGCCAATGGACCGATTTGGCATCGGTCTGGGCCTCACCCTTGCAGTGAAAGAAAGCGCATTTCTGCTGTGGATCTTAGCAGCAGTCTTGAGCGAAAAATGGCTGTTGCAGCAGGTCATTGTGCTGGATTCGCTGGGCTACAGCCGCTgg                                                                                     >  1:312733/1‑151 (MQ=255)                                                               cGCCAATGGACCGATTTGGCATCGGTCTGGGCCTCACCCTTGCAGTGAAAGAAAGCGCATTTCTGCTGTGGATCTTAGCAGCAGTCTTGAGCGAAAAATGGCTGTTGCAGCAGGTCATTGTGCTGGATTCGCTGGGCTACAGCCGCTGGCa                                                                                   <  1:769711/151‑1 (MQ=255)                                                                 ccAATGGACCGATTTGGCATCGGTCTGGGCCTCACCCTTGCAGTGAAAGAAAGCGCATTTCTGCTGTGGATCTTAGCAGCAGTCTTGAGCGAAAAATGGCTGTTGCAGCAGGTCATTGTGCTGGATTCGCTGGGCTACAGCCGCTGGCAAt                                                                                 >  2:823887/1‑151 (MQ=255)                                                                  cAATGGACCGATTTGGCATCGGTCTGGGCCTCACCCTTGCAGTGAAAGAAAGCGCATTTCTGCTGTGGATCTTAGCAGCAGTCTTGAGCGAAAAATGGCTGTTGCAGCAGGTCATTGTGCTGGATTCGCTGGGCTACAGCCGCTGGCAAt                                                                                 >  2:389020/1‑150 (MQ=255)                                                                     tGGACCGATTTGGCATCGGTCTGGGCCTCACCCTTGCAGTGAAAGAAAGCGCATTTCTGCTGTGGATCTTAGCAGCAGTCTTGAGCGAAAAATGGCTGTTGCAGCAGGTCATTGTGCTGGATTCGCTGGGCTACAGCCGCTGGCAATGCCt                                                                             >  2:663467/1‑151 (MQ=255)                                                                      ggACCGATTTGGCATCGGTCTGGGCCTCACCCTTGCAGTGAAAGAAAGCGCATTTCTGCTGTGGATCTTAGCAGCAGTCTTGAGCGAAAAATGGCTGTTGCAGCAGGTCATTGTGCTGGATTCGCTGGGCTACAGCCGCTGGCAATGCCTg                                                                            <  2:87876/151‑1 (MQ=255)                                                                       gACCGATTTGGCATCGGTCTGGGCCTCACCCTTGCAGTGAAAGAAAGCGCATTTCTGCTGTGGATCTTAGCAGCAGTCTTGAGCGAAAAATGGCTGTTGCAGCAGGTCATTGTGCTGGATTCGCTGGGCTACAGCCGCTGGCAATGCCTGa                                                                           <  2:534013/151‑1 (MQ=255)                                                                            aTTTGGCATCGGTCTGGGCCTCACCCTTGCAGTGAAAGAAAGCGCATTTCTGCTGTGGATCTTAGCAGCAGTCTTGAGCGAAAAATGGCTGTTGCAGCAGGTCATTGTGCTGGATTCGCTGGGCTACAGCCGCTGGCAATGCCTGAACTgg                                                                      <  1:723207/151‑1 (MQ=255)                                                                                        tCTGGGCCTCACCCTTGCAGTGAAAGAAAGCGCATTTCTGCTGTGGATCTTAGCAGCAGTCTTGAGCGAAAAATGGCTGTTGCAGCAGGTCATTGTGCTGGATTCGCTGGGCTACAGCCGCTGGCAATGCCTGAACTGGCTGCTGTTGccc                                                          >  2:795686/1‑151 (MQ=255)                                                                                            ggCCTCACCCTTGCAGTGAAAGAAAGCGCATTTCTGCTGTGGATCTTAGCAGCAGTCTTGAGCGAAAAATGGCTGTTGCAGCAGGTCATTGTGCTGGATTCGCTGGGCTACAGCCGCTGGCAATGCCTGAACTGGCTGCTGTTGCCCTCCg                                                      >  1:839995/1‑151 (MQ=255)                                                                                               cTCACCCTTGCAGTGAAAGAAAGCGCATTTCTGCTGTGGATCTTAGCAGCAGTCTTGAGCGAAAAATGGCTGTTGCAGCAGGTCATTGTGCTGGATTCGCTGGGCTACAGCCGCTGGCAATGCCTGAACTGGCTGCTGTTGCCCTCCGTcg                                                   >  2:446777/1‑151 (MQ=255)                                                                                                          aGTGAAAGAAAGCGCATTTCTGCTGTGGATCTTAGCAGCAGTCTTGAGCGAAAAATGGCTGTTGCAGCAGGTCATTGTGCTGGATTCGCTGGGCTACAGCCGCTGGCAATGCCTGAACTGGCTGCTGTTGCCCTCCGTCGCGCCTGCGCTg                                        >  2:281397/1‑151 (MQ=255)                                                                                                            tGAAAGAAAGCGCATTTCTGCTGTGGATCTTAGCAGCAGTCTTGAGCGAAAAATGGCTGTTGCAGCAGGTCATTGTGCTGGATTCGCTGGGCTACAGCCGCTGGCAATGCCTGAACTGGCTGCTGTTGCCCTCCGTCGCGCCTGCGCTGGc                                      >  1:667831/1‑151 (MQ=255)                                                                                                            cgaaagaaaGCGCATTTCTGCTGTGGATCTTAGCAGCAGACTTGAGCGAAAAATGGCTGTTGCAGCAGGTCATTGTGCTGGATTCGCTGGGCTACAGCCGCTGGCAATGCCTGAACTGGCTGCTGTTGCCCTCCGTCGCGCCTGCGCTGGc                                      >  1:43713/2‑151 (MQ=255)                                                                                                              aaagaaagCGCATTTCTGCTGTGGATCTTAGCAGCAGTCTTGAGCGAAAAATGGCTGTTGCAGCAGGTCATTGTGCTGGATTCGCTGGGCTACAGCCGCTGGCAATGCCTGAACTGGCTGCTGTTGCCCTCCGTCGCGCCTGCGCTGGCa                                     >  1:769723/1‑150 (MQ=255)                                                                                                                         aTTTCTGCTGTGGATCTTAGCAGCAGTCTTGAGCGAAAAATGGCTGTTGCAGCAGGTCATTGTGCTGGATTCGCTGGGCTACAGCCGCTGGCAATGCCTGAACTGGCTGCTGTTGCCCTCCGTCGCGCCTGCGCTGGCAATGGCGATGCTg                         >  2:151784/1‑151 (MQ=255)                                                                                                                             ctgctgTGGATCTTAGCAGCAGTCTTGAGCGAAAAATGGCTGTTGCAGCAGGTCATTGTGCTGGATTCGCTGGGCTACAGCCGCTGGCAATGCCTGAACTGGCTGCTGTTGCCCTCCGTCGCGCCTGCGCTGGCAATGGCGATGCTGGCg                      >  2:562076/1‑150 (MQ=255)                                                                                                                              tgctgTGGATCTTAGCAGCAGTCTTGAGCGAAAAATGGCTGTTGCAGCAGGTCATTGTGCTGGATTCGCTGGGCTACAGCCGCTGGCAATGCCTGAACTGGCTGCTGTTGCCCTCCGTCGCGCCTGCGCTGGCAATGGCGATGCTGGCGAt                    >  2:324467/1‑151 (MQ=255)                                                                                                                               gctgTGGATCTTAGCAGCAGTCTTGAGCGAAAAATGGCTGTTGCAGCAGGTCATTGTGCTGGATTCGCTGGGCTACAGCCGCTGGCAATGCCTGAACTGGCTGCTGTTGCCCTCCGTCGCGCCTGCGCTGGCAATGGCGATGCTGGCg                      <  2:724954/148‑1 (MQ=255)                                                                                                                                      aTCTTAGCAGCAGTCTTGAGCGAAAAATGGCTGTTGCAGCAGGTCATTGTGCTGGATTCGCTGGGCTACAGCCGCTGGCAATGCCTGAACTGGCTGCTGTTGCCCTCCGTCGCGCCTGCGCTGGCAATGGCGATGCTGGCGATTGTTGCCt            >  1:84532/1‑151 (MQ=255)                                                                                                                                       tCTTAGCAGCAGTCTTGAGCGAAAAATGGCTGTTGCAGCAGGTCATTGTGCTGGATTCGCTGGGCTACAGCCGCTGGCAATGCCTGAACTGGCTGCTGTTGCCCTCCGTCGCGCCTGCGCTGGCAATGGCGATGCTGGCGATTGTTGcc             >  1:588749/1‑149 (MQ=255)                                                                                                                                          tAGCAGCAGTCTTGAGCGAAAAATGACTGTTGCAGCAGGTCATTGTGCTGGATTCGCGGGGCTACAGCCTCTGGCAATGCCTGAACTGGCTGCTGTTGCCCTCCGTCGAGCCTGCGCTGGCATTGGCGATGCTGGCGATTGTTGCCTgttg        >  1:68084/1‑148 (MQ=255)                                                                                                                                           agcagcAGTCTTGAGCGAAAAATGGCTGTTGCAGCAGGTCATTGTGCTGGATTCGCTGGGCTACAGCCGCTGGCAATGCCTGAACTGGCTGCTGTTGCCCTCCGTCGCGCCTGCGCTGGCAATGGCGATGCTGGCGATTGTTGCCTGGTc        <  1:896259/150‑1 (MQ=255)                                                                                                                                           agcagcAGTCTTGAGCGAAAAATGGCTGTTGCAGCAGGTCATTGTGCTGGATTCGCTGGGCTACAGCCGCTGGCAATGCCTGAACTGGCTGCTGTTGCCCTCCGTCGCGCCTGCGCTGGCAATGGCGATGCTGGCGATTGTTGCCTGGTCg       >  2:307953/1‑151 (MQ=255)                                                                                                                                           agcagcAGTCTTGAGCGAAAAATGGCTGTTGCAGCAGGTCATTGTGCTGGATTCGCTGGGCTACAGCCGCTGGCAATGCCTGAACTGGCTGCTGTTGCCCTCCGTCGCGCCTGCGCTGGCAATGGCGATGCTGGCGATTGTTGCCTGGTCg       <  1:692457/151‑1 (MQ=255)                                                                                                                                                cagTCTTGAGCGAAAAATGGCTGTTGCAGCAGGTCATTGTGCTGGATTCGCTGGGCTACAGCCGCTGGCAATGCCTGAACTGGCTGCTGTTGCCCTCCGTCGCGCCTGCGCTGGCAATGGCGATGCTGGCGATTGTTGCCTGGTCGCTgtc  >  1:756959/1‑151 (MQ=255)                                                                                                                                                    |                                                                                                                                                   CAGCGCCCTTCTGCTCTTTGCTGACGGAGGGCTGCTTTATGACTATTTCCCGTATTTCACTCCGCCAATGGACCGATTTGGCATCGGTCTGGGCCTCACCCTTGCAGTGAAAGAAAGCGCATTTCTGCTGTGGATCTTAGCAGCAGTATTGAGCGAAAAATGGCTGTTGCAGCAGGTCATTGTGCTGGATTCGCTGGGCTACAGCCGCTGGCAATGCCTGAACTGGCTGCTGTTGCCCTCCGTCGCGCCTGCGCTGGCAATGGCGATGCTGGCGATTGTTGCCTGGTCGCTGTC  >  NC_000913/1837526‑1837819

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑