Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,880,386	A→C	L125R (CTG→CGG)	leuE ←	leucine efflux protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,880,386	0	A	C	100.0%	196.8 / NA	54	L125R (CTG→CGG)	leuE	leucine efflux protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (26/28);  total (26/28)

ATCAGGAAGCTCAAATAGCAGAAACTCACCAGTTCCAGCGTCGCCGCCAGAATAAAGAATGAAATTCCCGTATGTGGGGCATTAACATCGATAAACTGTACGAAAAACGACACATAGAACAAAATGGCTTTCGGATTAGTCAGGCTCAAAATTAACGCGCGTTTAAAAATAGCACCGTATTGGGGCTCATCGGATTTGGCCTCGCTATTTTTACCCTTCAGGGTCGCGTAAAGAATTTTACTCCCCAGATAGAGCAAATAAAACGCACCAAGATAACGTACAATGTT  >  NC_000913/1880245‑1880531                                                                                                                                              |                                                                                                                                                  aTCAGGAAGCTCAAATAGCAGAAACTCACCAGTTCCAGCGTCGCCGCCAGAATAAAGAATGAAATTCCTGTATGTGGGGCATTAACATCGATAAACTGTACGAAAAACGACACATAGAACAAAATGGCTTTCGGATTAGTCCGGCTCaaa                                                                                                                                           <  2:737274/150‑1 (MQ=255)   cAGGAAGCTCAAATAGCAGAAACTCACCAGTTCCAGCGTCGCCGCCAGAATAAAGAATGAAATTCCCGTATGTGGGGCATTAACATCGATAAACTGTACGAAAAACGACACATAGAACAAAATGGCTTTCGGATTAGTCCGGCTCAAAAt                                                                                                                                         <  2:62740/150‑1 (MQ=255)     ggAAGCTCAAATAGCAGAAACTCACCAGTTCCAGCGTCGCCGCCAGAATAAAGAATGAAATTCCCGTATGTGGGGCATTAACATCGATAAACTGTACGAAAAACGACACATAGAACAAAATGGCTTTCGGATTAGTCCGGCTCAAAATTa                                                                                                                                       >  2:496643/1‑150 (MQ=255)       aaGCTCAAATAGCAGAAACTCACCAGTTCCAGCGTCGCCGCCAGAATAAAGAATGAAATTCCCGTATGTGGGGCATTAACATCGATAAACTGTACGAAAAACGACACATAGAACAAAATGGCTTTCGGATTAGTCCGGCTCAAAATTAAcg                                                                                                                                    >  1:881690/1‑151 (MQ=255)             aaaTAGCAGAAACTCACCAGTTCCAGCGTCGCCGCCAGAATAAAGAATGAAATTCCCGTATGTGGGGCATTAACATCGATAAACTGTACGAAAAACGACACATAGAACAAAATGGCTTTCGGATTAGTCCGGCTCAAAATTAACGCGCGtt                                                                                                                              >  2:397282/1‑151 (MQ=255)             aaaTAGCAGAAACTCACCAGTTCCAGCGTCGCCGCCAGAATAAAGAATGAAATTCCCGTATGTGGGGCATTAACATCGATAAACTGTACGAAAAACGACACATAGAACAAAATGGCTTTCGGATTAGTCCGGCTCAAAATTAACGCGCGtt                                                                                                                              <  2:491586/151‑1 (MQ=255)                 aGCAGAAACTCACCAGTTCCAGCGTCGCCGCCAGAATAAAGAATGAAATTCCCGTATGTGGGGCATTAACATCGATAAACTGTACGAAAAACGACACATAGAACAAAATGGCTTTCGGATTAGTCCGGCTCAAAATTAACGCGCGTTTaa                                                                                                                           <  1:696755/150‑1 (MQ=255)                 aGCAGAAACTCACCAGTTCCAGCGTCGCCGCCAGAATAAAGAATGAAATTCCCGTATGTGGGGCATTAACATCGATAAACTGTACGAAAAACGACACATAGAACAAAATGGCTTTCGGATTAGTCCGGCTCAAAATTAACGCGCGTTTaa                                                                                                                           <  2:793883/150‑1 (MQ=255)                       aaCTCACCAGTTCCAGCGTCGCCGCCAGAATAAAGAATGAAATTCCCGTATGTGGGGCATTAACATCGATAAACTGTACGAAAAACGACACATAGAACAAAATGGCTTTCGGATTAGTCCGGCTCAAAATTAACGCGCGTTTAAAAATAGc                                                                                                                    >  2:893638/1‑151 (MQ=255)                            aCCAGTTCCAGCGTCGCCGCCAGAATAAAGAATGAAATTCCCGTATGTGGGGCATTAACATCGATAAACTGTACGAAAAACGACACATAGAACAAAATGGCTTTCGGATTAGTCCGGCTCAAAATTAACGCGCGTTTAAAAATAGCACCGt                                                                                                               <  1:481757/151‑1 (MQ=255)                             ccAGTTCCAGCGTCGCCGCCAGAATAAAGAATGAAATTCCCGTATGTGGGGCATTAACATCGATAAACTGTACGAAAAACGACACATAGAACAAAATGGCTTTCGGATTAGTCCGGCTCAAAATTAACGCGCGTTTAAAAATAGCACCGTa                                                                                                              <  1:666395/151‑1 (MQ=255)                                gTTCCAGCGTCGCCGCCAGAATAAAGAATGAAATTCCCGTATGTGGGGCATTAACATCGATAAACTGTACGAAAAACGACACATAGAACAAAATGGCTTTCGGATTAGTCCGGCTCAAAATTAACGCGCGTTTAAAAATAGCACCGTATTg                                                                                                           <  2:8373/151‑1 (MQ=255)                                  tCCAGCGTCGCCGCCAGAATAAAGAATGAAATTCCCGTATGTGGGGCATTAACATCGATAAACTGTACGAAAAACGACACATAGAACAAAATGGCTTTCGGATTAGTCCGGCTCAAAATTAACGCGCGTTTAAAAATAGCACCGTATTgg                                                                                                          <  1:308555/150‑1 (MQ=255)                                    cAGCGTCGCCGCCAGAATAAAGAATGAAATTCCCGTATGTGGGGCATTAACATCGATAAACTGTACGAAAAACGACACATAGAACAAAATGGCTTTCGGATTAGTCCGGCTCAAAATTAACGCGCGTTTAAAAATAGCACCGTATTGGGGc                                                                                                       >  1:588416/1‑151 (MQ=255)                                      gCGTCGCCGCCAGAATAAAGAATGAAATTCCCGTATGTGGGGCATTAACATCGATAAACTGTACGAAAAACGACACATAGAACAAAATGGCTTTCGGATTAGTCCGGCTCAAAATTAACGCGCGTTTAAAAATAGCACCGTATTGGGGCTc                                                                                                     >  2:318199/1‑151 (MQ=255)                                             cgccAGAATAAAGAATGAAATTCCCGTATGTGGGGCATTAACATCGATAAACTGTACGAAAAACGACACATAGAACAAAATGGCTTTCGGATTAGTCCGGCTCAAAATTAACGCGCGTTTAAAAATAGCACCGTATTGGGGCTCATCGGAt                                                                                              <  1:743183/151‑1 (MQ=255)                                              gccAGAATAAAGAATGAAATTCCCGTATGTGGGGCATTAACATCGATAAACTGTACGAAAAACGACACATAGAACAAAATGGCTTTCGGATTAGTCCGGCTCAAAATTAACGCGCGTTTAAAAATAGCACCGTATTGGGGCTCATCGGAtt                                                                                             >  1:448806/1‑151 (MQ=255)                                                 aGAATAAAGAATGAAATTCCCGTATGTGGGGCATTAACATCGATAAACTGTACGAAAAACGACACATAGAACAAAATGGCTTTCGGATTAGTCCGGCTCAAAATTAACGCGCGTTTAAAAATAGCACCGAATTGGGGCTCATCGGAttt                                                                                            >  1:785762/1‑149 (MQ=255)                                                    aTAAAGAATGAAATTCCCGTATGTGGGGCATTAACATCGATAAACTGTACGAAAAACGACACATAGAACAAAATGGCTTTCGGATTAGTCCGGCTCAAAATTAACGCGCGTTTAAAAATAGCACCGTATTGGGGCTCCTCGGATTTGGcc                                                                                        <  1:673489/150‑1 (MQ=255)                                                      aaaGAATGAAATTCCCGTATGTGGGGCATTAACATCGATAAACTGTACGAAAAACGACACATAGAACAAAATGGCTTTCGGATTAGTCCGGCTCAAAATTAACGCGCGTTTAAAAATAGCACCGTATTGGGGCTCATCGGATTTGGCCTc                                                                                      <  1:17533/150‑1 (MQ=255)                                                      aaaGAATGAAATTCCCGTATGTGGGGCATTAACATCGATAAACTGTACGAAAAACGACACATAGAACAAAATGGCTTTCGGATTAGTCCGGCTCAAAATTAACGCGCGTTTAAAAATAGCACCGTATTGGGGCTCATCGGATTTGGCCTCg                                                                                     >  2:23320/1‑151 (MQ=255)                                                       aaGAATGAAATTCCCGTATGTGGGGCATTAACATCGATAAACTGTACGAAAAACGACACATAGAACAAAATGGCTTTCGGATTAGTCCGGCTCAAAATTAACGCGCGTTTAAAAATAGCACCGTATTGGGGCTCATCGGATTTGGCCTc                                                                                      >  1:448165/1‑149 (MQ=255)                                                            tGAAATTCCCGTATGTGGGGCATTAACATCGATAAACTGTACGAAAAACGACACATAGAACAAAATGGCTTTCGGATTAGTCCGGCTCAAAATTAACGCGCGTTTAAAAATAGCACCGTATTGGGGCTCATCGGATTTGGCCTCGCTAtt                                                                                >  2:98449/1‑150 (MQ=255)                                                             gAAATTCCCGTATGTGGGGCATTAACATCGATAAACTGTACGAAAAACGACACATAGAACAAAATGGCTTTCGGATTAGTCCGGCTCAAAATTAACGCGCGTTTAAAAATAGCACCGTATTGGGGCTCATCGGATTTGGCCTCGCTAtttt                                                                              <  2:167037/151‑1 (MQ=255)                                                                          gtgGGGCATTAACATCGATAAACTGTACGAAAAACGACACATAGAACAAAATGGCTTTCGGATTAGTCCGGCTCAAAATTAACGCGCGTTTAAAAATAGCACCGTATTGGGGCTCATCGGATTTGGCCTCGCTATTTTTACCCTTCAGGGt                                                                 >  1:867668/1‑151 (MQ=255)                                                                            ggggCATTAACATCGATAAACTGTACGAAAAACGACACATAGAACAAAATGGCTTTCGGATTAGTCCGGCTCAAAATTAACGCGCGTTTAAAAATAGCACCGTATTGGGGCTCATCGGATTTGGCCTCGCTATTTTTACCCTTCAGGGTcg                                                               >  2:632041/1‑151 (MQ=255)                                                                                cATTAACATCGATAAACTGTACGAAAAACGACACATAGAACAAAATGGCTTTCGGATTAGTCCGGCTCAAAATTAACGCGCGTTTAAAAATAGCACCGTATTGGGGCTCATCGGATTTGGCCTCGCTATTTTTACCCTTCAGTGTCGCGTa                                                           <  2:889755/151‑1 (MQ=255)                                                                                 aTTAACATCGATAAACTGTACGAAAAACGACACATAGAACAAAATGGCTTTCGGATTAGTCCGGCTCAAAATTAACGCGCGTTTAAAAATAGCACCGTATTGGGGCTCATCGGATTTGGCCTCGCTATTTTTACCCTTCAGGGTCGCGTaa                                                          <  2:533993/151‑1 (MQ=255)                                                                                     aCATCGATAAACTGTACGAAAAACGACACATAGAACAAAATGGCTTTCGGATTAGTCCGGCTCAAAATTAACGCGCGTTTAAAAATAGCACCGTATTGGGGCTCATCGGATTTGGCCTCGCTATTTTTACCCTTCAGGGTCGCGTAAAGa                                                       >  1:338564/1‑150 (MQ=255)                                                                                         cGATAAACTGTACGAAAAACGACACATAGAACAAAATGGCTTTCGGATTAGTCCGGCTCAAAATTAACGCGCGTTTAAAAATAGCACCGTATTGGGGCTCATCGGATTTGGCCTCGCTATTTTTACCCTTCAGGGTCGCGTAAAGAAtttt                                                  >  1:777048/1‑151 (MQ=255)                                                                                           aTAAACTGTACGAAAAACGACACATAGAACAAAATGGCTTTCGGATTAGTCCGGCTCAAAATTAACGCGCGTTTAAAAATAGCACCGTATTGGGGCTCATCGGATTTGGCCTCGCTATTTTTACCCTTCAGGGTCGCGTAAAGAATTTTAc                                                <  2:546557/151‑1 (MQ=255)                                                                                              aaCTGTACGAAAAACGACACATAGAACAAAATGGCTTTCGGATTAGTCCGGCTCAAAATTAACGCGCGTTTAAAAATAGCACCGTATTGGGGCTCATCGGATTTGGCCTCGCTATTTTTACCCTTCAGGGTCGCGTAAAGAATTTTACTcc                                             >  1:709660/1‑151 (MQ=255)                                                                                                  gTACGAAAAACGACACATAGAACAAAATGGCTTTCGGATTAGTCCGGCTCAAAATTAACGCGCGTTTAAAAATAGCACCGTATTGGGGCTCATCGGATTTGGCCTCGCTATTTTTACCCTTCAGGGTCGCGTAAAGAATTTTACTCCCCAg                                         <  1:311014/151‑1 (MQ=255)                                                                                                    aCGAAAAACGACACATAGAACAAAATGGCTTTCGGATTAGTCCGGCTCAAAATTAACGCGCGTTTAAAAATAGCACCGTATTGGGGCTCATCGGATTTGGCCTCGCTATTTTTACCCTTCAGGGTCGCGTAAAGAATTTTACTcccc                                           >  1:928706/1‑147 (MQ=255)                                                                                                       aaaaaCGACACATAGAACAAAATGGCTTTCGGATTAGTCCGGCTCAAAATTAACGCGCGTTTAAAAATAGCACCGTATTGGGGCTCATCGGATTTGGCCTCGCTATTTTTACCCTTCAGGGTCGCGTAAAGAATTTTACTCCCCAGATaga                                    <  2:916801/151‑1 (MQ=255)                                                                                                        aaaaCGACACATAGAACAAAATGGCTTTCGGATTAGTCCGGCTCAAAATTAACGCGCGTTTAAAAATAGCACCGTATTGGGGCTCATCGGATTTGGCCTCGCTATTTTTACCCTTCAGGGTCGCGTAAAGAATTTTACTCCCCAGATagag                                   <  2:814276/151‑1 (MQ=255)                                                                                                           aCGACACATAGAACAAAATGGCTTTCGGATTAGTCCGGCTCAAAATTAACGCGCGTTTAAAAATAGCACCGTATTGGGGCTCATCGGATTTGGCCTCGCTATTTTTACCCTTCAGGGTCGCGTAAAGAATTTTACTCCCCAGATAGAGCa                                 >  2:477452/1‑150 (MQ=255)                                                                                                           aCGACACATAGAACAAAATGGCTTTCGGATTAGTCCGGCTCAAAATTAACGCGCGTTTAAAAATAGCACCGTATTGGGGCTCATCGGATTTGGCCTCGCTATTTTTACCCTTCAGGGTCGCGTAAAGAATTTTACTCCCCAGATAGAGCa                                 >  1:428147/1‑150 (MQ=255)                                                                                                              acacATAGAACAAAATGGCTTTCGGATTAGTCCGGCTCAAAATTAACGCGCGTTTAAAAATAGCACCGTATTGGGGCTCATCGGATTTGGCCTCGCTATTTTTACCCTTCAGGGTCGCGTAAAGAATTTTACTCCCCAGATAGAGCAAATa                             <  1:757280/151‑1 (MQ=255)                                                                                                                acaTAGAACAAAATGGCTTTCGGATTAGTCCGGCTCAAAATTAACGCGCGTTTAAAAATAGCACCGTATTGGGGCTCATCGGATTTGGCCTCGCTATTTTTACCCTTCAGGGTCGCGTAAAGAATTTTACTCCCCAGATAGAGCAAATaaa                           <  2:449179/151‑1 (MQ=255)                                                                                                                    aGAACAAAATGGCTTTCGGATTAGTCCGGCTCAAAATTAACGCGCGTTTAAAAATAGCACCGTATTGGGGCTCATCGGATTTGGCCTCGCTATTTTTACCCTTCAGGGTCGCGTAAAGAATTTTACTCCCCAGATAGAGCAAATAAAACGc                       <  1:384560/151‑1 (MQ=255)                                                                                                                    aGAACAAAATGGCTTTCGGATTAGTCCGGCTCAAAATTAACGCGCGTTTAAAAATAGCACCGTATTGGGGCTCATCGGATTTGGCCTCGCTATTTTTACCCTTCAGGGTCGCGTAAAGAATTTTACTCCCCAGATAGAGCAAATAAAACGc                       >  2:315474/1‑151 (MQ=255)                                                                                                                     gAACAAAATGGCTTTCGGATTAGTCCGGCTCAAAATTAACGCGCGTTTAAAAATAGCACCGTATTGGGGCTCATCGGATTTGGCCTCGCTATTTTTACCCTTCAGGGTCGCGTAAAGAATTTTACTCCCCAGATAGAGCAAATAAAACGCa                      <  1:412306/151‑1 (MQ=255)                                                                                                                        cAAAATGGCTTTCGGATTAGTCCGGCTCAAAATTAACGCGCGTTTAAAAATAGCACCGTATTGGGGCTCATCGGATTTGGCCTCGCTATTTTTACCCTTCAGGGTCGCGTAAAGAATTTTACTCCCCAGATAGAGCAAATAAAACGCACCa                   <  2:638424/151‑1 (MQ=255)                                                                                                                            aTGGCTTTCGGATTAGTCCGGCTCAAAATTAACGCGCGTTTAAAAATAGCGCCGTATTGGGGCTCATCGGATTTGGCCTCGCTATTTTTACCCTGCAAGGTCGCGTAAAGAATTTTACTCCCCAGATAGAGCAAATAAAACGCACCAAGAt               <  2:785782/151‑1 (MQ=255)                                                                                                                              ggCTTTCGGATTAGTCCGGCTCAAAATTAACGCGCGTTTAAAAATAGCACCGTATTGGGGCTCAACGGATTTGGCCTCGCTATTTTTTCCCGTCAGGGTCGCGTAAAGAATTTTACTCCCCAGATAGAGCAAATAAAACGCACCAAGATaa             <  2:763557/151‑1 (MQ=255)                                                                                                                               gCTTTCGGATTAGTCCGGCTCAAAATTAACGCGCGTTTAAAAATAGCACCGTATTGGGGCTCATCGGATTTGGCCTCGCTATTTTTACCCTTCAGGGTCGCGTAAAGAATTTTACTCCCCAGATAGAGCAAATAAAACGCACCAAGATAAc            <  2:530269/151‑1 (MQ=255)                                                                                                                               gCTTTCGGATTAGTCCGGCTCAAAATTAACGCGCGTTTAAAAATAGCACCGTATTGGGGCTCATCGGATTTGGCCTCGCTATTTTTACCCTTCAGGGTCGCGTAAAGAATTTTACTCCCCAGATAGAGCAAATAAAACGCACCAAGATAAc            <  1:460585/151‑1 (MQ=255)                                                                                                                               gCTTTCGGATTAGTCCGGCTCAAAATTAACGCGCGTTTAAAAATAGCACCGTATTGGGGCTCATCGGATTTGGCCTCGCTATTTTTACCCTTCAGGGTCGCGTAAAGAATTTTACTCCCCAGATAGAGCAAATAAAACGCACCAAGATAAc            >  2:381076/1‑151 (MQ=255)                                                                                                                                cTTTCGGATTAGTCCGGCTCAAAATTAACGCGCGTTTAAAAATAGCACCGTATTGGGGCTCATCGGATTTGGCCTCGCTATTTTTACCCTTCAGGGTCGCGTAAAGAATTTTACTCCCCAGATAGAGCAAATAAAACGCACCAAGATAACg           >  2:366301/1‑151 (MQ=255)                                                                                                                                 tttCGGATTAGTCCGGCTCAAAATTAACGCGCGTTTAAAAATAGCACCGTATTGGGGCTCATCGGATTTGGCCTCGCTATTTTTACCCTTCAGGGTCGCGTAAAGAATTTTACTCCCCAGATAGAGCAAATAAAACGCACCAAGATAACGt          >  1:590748/1‑151 (MQ=255)                                                                                                                                       aTTAGTCCGGCTCAAAATTAACGCGCGTTTAAAAATAGCACCGTATTGGGGCTCATCGGATTTGGCCTCGCTATTTTTACCCTTCAGGGTCGCGTAAAGAATTTTACTCCCCAGATAGAGCAAATAAAACGCACCAAGATAACGTACAATg    >  2:438469/1‑151 (MQ=255)                                                                                                                                        ttAGTCCGGCTCAAAATTAACGCGCGTTTAAAAATAGCACCGTATTGGGGCTCATCGGATTTGGCCTCGCTATTTTTACCCTTCAGGGTCGCGTAAAGAATTTTACTCCCCAGATAGAGCAAATAAAACGCACCAAGATAACGTACAATGt   >  2:346236/1‑151 (MQ=255)                                                                                                                                         tAGTCCGGCTCAAAATTAACGCGCGTTTAAAAATAGCACCGTATTGGGGCTCATCGGATTTGGCCTCGCTATTTTTACCCTTCAGGGTCGCGTAAAGAATTTTACTCCCCAGATAGAGCAAATAAAACGCACCAAGATAACGTACAATGtt  >  1:114994/1‑151 (MQ=255)                                                                                                                                              |                                                                                                                                                  ATCAGGAAGCTCAAATAGCAGAAACTCACCAGTTCCAGCGTCGCCGCCAGAATAAAGAATGAAATTCCCGTATGTGGGGCATTAACATCGATAAACTGTACGAAAAACGACACATAGAACAAAATGGCTTTCGGATTAGTCAGGCTCAAAATTAACGCGCGTTTAAAAATAGCACCGTATTGGGGCTCATCGGATTTGGCCTCGCTATTTTTACCCTTCAGGGTCGCGTAAAGAATTTTACTCCCCAGATAGAGCAAATAAAACGCACCAAGATAACGTACAATGTT  >  NC_000913/1880245‑1880531

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑