Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,880,386 A→C L125R (CTG→CGG)  leuE ← leucine efflux protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,880,3860AC100.0% 196.8 / NA 54L125R (CTG→CGG) leuEleucine efflux protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (26/28);  total (26/28)

ATCAGGAAGCTCAAATAGCAGAAACTCACCAGTTCCAGCGTCGCCGCCAGAATAAAGAATGAAATTCCCGTATGTGGGGCATTAACATCGATAAACTGTACGAAAAACGACACATAGAACAAAATGGCTTTCGGATTAGTCAGGCTCAAAATTAACGCGCGTTTAAAAATAGCACCGTATTGGGGCTCATCGGATTTGGCCTCGCTATTTTTACCCTTCAGGGTCGCGTAAAGAATTTTACTCCCCAGATAGAGCAAATAAAACGCACCAAGATAACGTACAATGTT  >  NC_000913/1880245‑1880531
                                                                                                                                             |                                                                                                                                                 
aTCAGGAAGCTCAAATAGCAGAAACTCACCAGTTCCAGCGTCGCCGCCAGAATAAAGAATGAAATTCCTGTATGTGGGGCATTAACATCGATAAACTGTACGAAAAACGACACATAGAACAAAATGGCTTTCGGATTAGTCCGGCTCaaa                                                                                                                                           <  2:737274/150‑1 (MQ=255)
  cAGGAAGCTCAAATAGCAGAAACTCACCAGTTCCAGCGTCGCCGCCAGAATAAAGAATGAAATTCCCGTATGTGGGGCATTAACATCGATAAACTGTACGAAAAACGACACATAGAACAAAATGGCTTTCGGATTAGTCCGGCTCAAAAt                                                                                                                                         <  2:62740/150‑1 (MQ=255)
    ggAAGCTCAAATAGCAGAAACTCACCAGTTCCAGCGTCGCCGCCAGAATAAAGAATGAAATTCCCGTATGTGGGGCATTAACATCGATAAACTGTACGAAAAACGACACATAGAACAAAATGGCTTTCGGATTAGTCCGGCTCAAAATTa                                                                                                                                       >  2:496643/1‑150 (MQ=255)
      aaGCTCAAATAGCAGAAACTCACCAGTTCCAGCGTCGCCGCCAGAATAAAGAATGAAATTCCCGTATGTGGGGCATTAACATCGATAAACTGTACGAAAAACGACACATAGAACAAAATGGCTTTCGGATTAGTCCGGCTCAAAATTAAcg                                                                                                                                    >  1:881690/1‑151 (MQ=255)
            aaaTAGCAGAAACTCACCAGTTCCAGCGTCGCCGCCAGAATAAAGAATGAAATTCCCGTATGTGGGGCATTAACATCGATAAACTGTACGAAAAACGACACATAGAACAAAATGGCTTTCGGATTAGTCCGGCTCAAAATTAACGCGCGtt                                                                                                                              >  2:397282/1‑151 (MQ=255)
            aaaTAGCAGAAACTCACCAGTTCCAGCGTCGCCGCCAGAATAAAGAATGAAATTCCCGTATGTGGGGCATTAACATCGATAAACTGTACGAAAAACGACACATAGAACAAAATGGCTTTCGGATTAGTCCGGCTCAAAATTAACGCGCGtt                                                                                                                              <  2:491586/151‑1 (MQ=255)
                aGCAGAAACTCACCAGTTCCAGCGTCGCCGCCAGAATAAAGAATGAAATTCCCGTATGTGGGGCATTAACATCGATAAACTGTACGAAAAACGACACATAGAACAAAATGGCTTTCGGATTAGTCCGGCTCAAAATTAACGCGCGTTTaa                                                                                                                           <  1:696755/150‑1 (MQ=255)
                aGCAGAAACTCACCAGTTCCAGCGTCGCCGCCAGAATAAAGAATGAAATTCCCGTATGTGGGGCATTAACATCGATAAACTGTACGAAAAACGACACATAGAACAAAATGGCTTTCGGATTAGTCCGGCTCAAAATTAACGCGCGTTTaa                                                                                                                           <  2:793883/150‑1 (MQ=255)
                      aaCTCACCAGTTCCAGCGTCGCCGCCAGAATAAAGAATGAAATTCCCGTATGTGGGGCATTAACATCGATAAACTGTACGAAAAACGACACATAGAACAAAATGGCTTTCGGATTAGTCCGGCTCAAAATTAACGCGCGTTTAAAAATAGc                                                                                                                    >  2:893638/1‑151 (MQ=255)
                           aCCAGTTCCAGCGTCGCCGCCAGAATAAAGAATGAAATTCCCGTATGTGGGGCATTAACATCGATAAACTGTACGAAAAACGACACATAGAACAAAATGGCTTTCGGATTAGTCCGGCTCAAAATTAACGCGCGTTTAAAAATAGCACCGt                                                                                                               <  1:481757/151‑1 (MQ=255)
                            ccAGTTCCAGCGTCGCCGCCAGAATAAAGAATGAAATTCCCGTATGTGGGGCATTAACATCGATAAACTGTACGAAAAACGACACATAGAACAAAATGGCTTTCGGATTAGTCCGGCTCAAAATTAACGCGCGTTTAAAAATAGCACCGTa                                                                                                              <  1:666395/151‑1 (MQ=255)
                               gTTCCAGCGTCGCCGCCAGAATAAAGAATGAAATTCCCGTATGTGGGGCATTAACATCGATAAACTGTACGAAAAACGACACATAGAACAAAATGGCTTTCGGATTAGTCCGGCTCAAAATTAACGCGCGTTTAAAAATAGCACCGTATTg                                                                                                           <  2:8373/151‑1 (MQ=255)
                                 tCCAGCGTCGCCGCCAGAATAAAGAATGAAATTCCCGTATGTGGGGCATTAACATCGATAAACTGTACGAAAAACGACACATAGAACAAAATGGCTTTCGGATTAGTCCGGCTCAAAATTAACGCGCGTTTAAAAATAGCACCGTATTgg                                                                                                          <  1:308555/150‑1 (MQ=255)
                                   cAGCGTCGCCGCCAGAATAAAGAATGAAATTCCCGTATGTGGGGCATTAACATCGATAAACTGTACGAAAAACGACACATAGAACAAAATGGCTTTCGGATTAGTCCGGCTCAAAATTAACGCGCGTTTAAAAATAGCACCGTATTGGGGc                                                                                                       >  1:588416/1‑151 (MQ=255)
                                     gCGTCGCCGCCAGAATAAAGAATGAAATTCCCGTATGTGGGGCATTAACATCGATAAACTGTACGAAAAACGACACATAGAACAAAATGGCTTTCGGATTAGTCCGGCTCAAAATTAACGCGCGTTTAAAAATAGCACCGTATTGGGGCTc                                                                                                     >  2:318199/1‑151 (MQ=255)
                                            cgccAGAATAAAGAATGAAATTCCCGTATGTGGGGCATTAACATCGATAAACTGTACGAAAAACGACACATAGAACAAAATGGCTTTCGGATTAGTCCGGCTCAAAATTAACGCGCGTTTAAAAATAGCACCGTATTGGGGCTCATCGGAt                                                                                              <  1:743183/151‑1 (MQ=255)
                                             gccAGAATAAAGAATGAAATTCCCGTATGTGGGGCATTAACATCGATAAACTGTACGAAAAACGACACATAGAACAAAATGGCTTTCGGATTAGTCCGGCTCAAAATTAACGCGCGTTTAAAAATAGCACCGTATTGGGGCTCATCGGAtt                                                                                             >  1:448806/1‑151 (MQ=255)
                                                aGAATAAAGAATGAAATTCCCGTATGTGGGGCATTAACATCGATAAACTGTACGAAAAACGACACATAGAACAAAATGGCTTTCGGATTAGTCCGGCTCAAAATTAACGCGCGTTTAAAAATAGCACCGAATTGGGGCTCATCGGAttt                                                                                            >  1:785762/1‑149 (MQ=255)
                                                   aTAAAGAATGAAATTCCCGTATGTGGGGCATTAACATCGATAAACTGTACGAAAAACGACACATAGAACAAAATGGCTTTCGGATTAGTCCGGCTCAAAATTAACGCGCGTTTAAAAATAGCACCGTATTGGGGCTCCTCGGATTTGGcc                                                                                        <  1:673489/150‑1 (MQ=255)
                                                     aaaGAATGAAATTCCCGTATGTGGGGCATTAACATCGATAAACTGTACGAAAAACGACACATAGAACAAAATGGCTTTCGGATTAGTCCGGCTCAAAATTAACGCGCGTTTAAAAATAGCACCGTATTGGGGCTCATCGGATTTGGCCTc                                                                                      <  1:17533/150‑1 (MQ=255)
                                                     aaaGAATGAAATTCCCGTATGTGGGGCATTAACATCGATAAACTGTACGAAAAACGACACATAGAACAAAATGGCTTTCGGATTAGTCCGGCTCAAAATTAACGCGCGTTTAAAAATAGCACCGTATTGGGGCTCATCGGATTTGGCCTCg                                                                                     >  2:23320/1‑151 (MQ=255)
                                                      aaGAATGAAATTCCCGTATGTGGGGCATTAACATCGATAAACTGTACGAAAAACGACACATAGAACAAAATGGCTTTCGGATTAGTCCGGCTCAAAATTAACGCGCGTTTAAAAATAGCACCGTATTGGGGCTCATCGGATTTGGCCTc                                                                                      >  1:448165/1‑149 (MQ=255)
                                                           tGAAATTCCCGTATGTGGGGCATTAACATCGATAAACTGTACGAAAAACGACACATAGAACAAAATGGCTTTCGGATTAGTCCGGCTCAAAATTAACGCGCGTTTAAAAATAGCACCGTATTGGGGCTCATCGGATTTGGCCTCGCTAtt                                                                                >  2:98449/1‑150 (MQ=255)
                                                            gAAATTCCCGTATGTGGGGCATTAACATCGATAAACTGTACGAAAAACGACACATAGAACAAAATGGCTTTCGGATTAGTCCGGCTCAAAATTAACGCGCGTTTAAAAATAGCACCGTATTGGGGCTCATCGGATTTGGCCTCGCTAtttt                                                                              <  2:167037/151‑1 (MQ=255)
                                                                         gtgGGGCATTAACATCGATAAACTGTACGAAAAACGACACATAGAACAAAATGGCTTTCGGATTAGTCCGGCTCAAAATTAACGCGCGTTTAAAAATAGCACCGTATTGGGGCTCATCGGATTTGGCCTCGCTATTTTTACCCTTCAGGGt                                                                 >  1:867668/1‑151 (MQ=255)
                                                                           ggggCATTAACATCGATAAACTGTACGAAAAACGACACATAGAACAAAATGGCTTTCGGATTAGTCCGGCTCAAAATTAACGCGCGTTTAAAAATAGCACCGTATTGGGGCTCATCGGATTTGGCCTCGCTATTTTTACCCTTCAGGGTcg                                                               >  2:632041/1‑151 (MQ=255)
                                                                               cATTAACATCGATAAACTGTACGAAAAACGACACATAGAACAAAATGGCTTTCGGATTAGTCCGGCTCAAAATTAACGCGCGTTTAAAAATAGCACCGTATTGGGGCTCATCGGATTTGGCCTCGCTATTTTTACCCTTCAGTGTCGCGTa                                                           <  2:889755/151‑1 (MQ=255)
                                                                                aTTAACATCGATAAACTGTACGAAAAACGACACATAGAACAAAATGGCTTTCGGATTAGTCCGGCTCAAAATTAACGCGCGTTTAAAAATAGCACCGTATTGGGGCTCATCGGATTTGGCCTCGCTATTTTTACCCTTCAGGGTCGCGTaa                                                          <  2:533993/151‑1 (MQ=255)
                                                                                    aCATCGATAAACTGTACGAAAAACGACACATAGAACAAAATGGCTTTCGGATTAGTCCGGCTCAAAATTAACGCGCGTTTAAAAATAGCACCGTATTGGGGCTCATCGGATTTGGCCTCGCTATTTTTACCCTTCAGGGTCGCGTAAAGa                                                       >  1:338564/1‑150 (MQ=255)
                                                                                        cGATAAACTGTACGAAAAACGACACATAGAACAAAATGGCTTTCGGATTAGTCCGGCTCAAAATTAACGCGCGTTTAAAAATAGCACCGTATTGGGGCTCATCGGATTTGGCCTCGCTATTTTTACCCTTCAGGGTCGCGTAAAGAAtttt                                                  >  1:777048/1‑151 (MQ=255)
                                                                                          aTAAACTGTACGAAAAACGACACATAGAACAAAATGGCTTTCGGATTAGTCCGGCTCAAAATTAACGCGCGTTTAAAAATAGCACCGTATTGGGGCTCATCGGATTTGGCCTCGCTATTTTTACCCTTCAGGGTCGCGTAAAGAATTTTAc                                                <  2:546557/151‑1 (MQ=255)
                                                                                             aaCTGTACGAAAAACGACACATAGAACAAAATGGCTTTCGGATTAGTCCGGCTCAAAATTAACGCGCGTTTAAAAATAGCACCGTATTGGGGCTCATCGGATTTGGCCTCGCTATTTTTACCCTTCAGGGTCGCGTAAAGAATTTTACTcc                                             >  1:709660/1‑151 (MQ=255)
                                                                                                 gTACGAAAAACGACACATAGAACAAAATGGCTTTCGGATTAGTCCGGCTCAAAATTAACGCGCGTTTAAAAATAGCACCGTATTGGGGCTCATCGGATTTGGCCTCGCTATTTTTACCCTTCAGGGTCGCGTAAAGAATTTTACTCCCCAg                                         <  1:311014/151‑1 (MQ=255)
                                                                                                   aCGAAAAACGACACATAGAACAAAATGGCTTTCGGATTAGTCCGGCTCAAAATTAACGCGCGTTTAAAAATAGCACCGTATTGGGGCTCATCGGATTTGGCCTCGCTATTTTTACCCTTCAGGGTCGCGTAAAGAATTTTACTcccc                                           >  1:928706/1‑147 (MQ=255)
                                                                                                      aaaaaCGACACATAGAACAAAATGGCTTTCGGATTAGTCCGGCTCAAAATTAACGCGCGTTTAAAAATAGCACCGTATTGGGGCTCATCGGATTTGGCCTCGCTATTTTTACCCTTCAGGGTCGCGTAAAGAATTTTACTCCCCAGATaga                                    <  2:916801/151‑1 (MQ=255)
                                                                                                       aaaaCGACACATAGAACAAAATGGCTTTCGGATTAGTCCGGCTCAAAATTAACGCGCGTTTAAAAATAGCACCGTATTGGGGCTCATCGGATTTGGCCTCGCTATTTTTACCCTTCAGGGTCGCGTAAAGAATTTTACTCCCCAGATagag                                   <  2:814276/151‑1 (MQ=255)
                                                                                                          aCGACACATAGAACAAAATGGCTTTCGGATTAGTCCGGCTCAAAATTAACGCGCGTTTAAAAATAGCACCGTATTGGGGCTCATCGGATTTGGCCTCGCTATTTTTACCCTTCAGGGTCGCGTAAAGAATTTTACTCCCCAGATAGAGCa                                 >  2:477452/1‑150 (MQ=255)
                                                                                                          aCGACACATAGAACAAAATGGCTTTCGGATTAGTCCGGCTCAAAATTAACGCGCGTTTAAAAATAGCACCGTATTGGGGCTCATCGGATTTGGCCTCGCTATTTTTACCCTTCAGGGTCGCGTAAAGAATTTTACTCCCCAGATAGAGCa                                 >  1:428147/1‑150 (MQ=255)
                                                                                                             acacATAGAACAAAATGGCTTTCGGATTAGTCCGGCTCAAAATTAACGCGCGTTTAAAAATAGCACCGTATTGGGGCTCATCGGATTTGGCCTCGCTATTTTTACCCTTCAGGGTCGCGTAAAGAATTTTACTCCCCAGATAGAGCAAATa                             <  1:757280/151‑1 (MQ=255)
                                                                                                               acaTAGAACAAAATGGCTTTCGGATTAGTCCGGCTCAAAATTAACGCGCGTTTAAAAATAGCACCGTATTGGGGCTCATCGGATTTGGCCTCGCTATTTTTACCCTTCAGGGTCGCGTAAAGAATTTTACTCCCCAGATAGAGCAAATaaa                           <  2:449179/151‑1 (MQ=255)
                                                                                                                   aGAACAAAATGGCTTTCGGATTAGTCCGGCTCAAAATTAACGCGCGTTTAAAAATAGCACCGTATTGGGGCTCATCGGATTTGGCCTCGCTATTTTTACCCTTCAGGGTCGCGTAAAGAATTTTACTCCCCAGATAGAGCAAATAAAACGc                       <  1:384560/151‑1 (MQ=255)
                                                                                                                   aGAACAAAATGGCTTTCGGATTAGTCCGGCTCAAAATTAACGCGCGTTTAAAAATAGCACCGTATTGGGGCTCATCGGATTTGGCCTCGCTATTTTTACCCTTCAGGGTCGCGTAAAGAATTTTACTCCCCAGATAGAGCAAATAAAACGc                       >  2:315474/1‑151 (MQ=255)
                                                                                                                    gAACAAAATGGCTTTCGGATTAGTCCGGCTCAAAATTAACGCGCGTTTAAAAATAGCACCGTATTGGGGCTCATCGGATTTGGCCTCGCTATTTTTACCCTTCAGGGTCGCGTAAAGAATTTTACTCCCCAGATAGAGCAAATAAAACGCa                      <  1:412306/151‑1 (MQ=255)
                                                                                                                       cAAAATGGCTTTCGGATTAGTCCGGCTCAAAATTAACGCGCGTTTAAAAATAGCACCGTATTGGGGCTCATCGGATTTGGCCTCGCTATTTTTACCCTTCAGGGTCGCGTAAAGAATTTTACTCCCCAGATAGAGCAAATAAAACGCACCa                   <  2:638424/151‑1 (MQ=255)
                                                                                                                           aTGGCTTTCGGATTAGTCCGGCTCAAAATTAACGCGCGTTTAAAAATAGCGCCGTATTGGGGCTCATCGGATTTGGCCTCGCTATTTTTACCCTGCAAGGTCGCGTAAAGAATTTTACTCCCCAGATAGAGCAAATAAAACGCACCAAGAt               <  2:785782/151‑1 (MQ=255)
                                                                                                                             ggCTTTCGGATTAGTCCGGCTCAAAATTAACGCGCGTTTAAAAATAGCACCGTATTGGGGCTCAACGGATTTGGCCTCGCTATTTTTTCCCGTCAGGGTCGCGTAAAGAATTTTACTCCCCAGATAGAGCAAATAAAACGCACCAAGATaa             <  2:763557/151‑1 (MQ=255)
                                                                                                                              gCTTTCGGATTAGTCCGGCTCAAAATTAACGCGCGTTTAAAAATAGCACCGTATTGGGGCTCATCGGATTTGGCCTCGCTATTTTTACCCTTCAGGGTCGCGTAAAGAATTTTACTCCCCAGATAGAGCAAATAAAACGCACCAAGATAAc            <  2:530269/151‑1 (MQ=255)
                                                                                                                              gCTTTCGGATTAGTCCGGCTCAAAATTAACGCGCGTTTAAAAATAGCACCGTATTGGGGCTCATCGGATTTGGCCTCGCTATTTTTACCCTTCAGGGTCGCGTAAAGAATTTTACTCCCCAGATAGAGCAAATAAAACGCACCAAGATAAc            <  1:460585/151‑1 (MQ=255)
                                                                                                                              gCTTTCGGATTAGTCCGGCTCAAAATTAACGCGCGTTTAAAAATAGCACCGTATTGGGGCTCATCGGATTTGGCCTCGCTATTTTTACCCTTCAGGGTCGCGTAAAGAATTTTACTCCCCAGATAGAGCAAATAAAACGCACCAAGATAAc            >  2:381076/1‑151 (MQ=255)
                                                                                                                               cTTTCGGATTAGTCCGGCTCAAAATTAACGCGCGTTTAAAAATAGCACCGTATTGGGGCTCATCGGATTTGGCCTCGCTATTTTTACCCTTCAGGGTCGCGTAAAGAATTTTACTCCCCAGATAGAGCAAATAAAACGCACCAAGATAACg           >  2:366301/1‑151 (MQ=255)
                                                                                                                                tttCGGATTAGTCCGGCTCAAAATTAACGCGCGTTTAAAAATAGCACCGTATTGGGGCTCATCGGATTTGGCCTCGCTATTTTTACCCTTCAGGGTCGCGTAAAGAATTTTACTCCCCAGATAGAGCAAATAAAACGCACCAAGATAACGt          >  1:590748/1‑151 (MQ=255)
                                                                                                                                      aTTAGTCCGGCTCAAAATTAACGCGCGTTTAAAAATAGCACCGTATTGGGGCTCATCGGATTTGGCCTCGCTATTTTTACCCTTCAGGGTCGCGTAAAGAATTTTACTCCCCAGATAGAGCAAATAAAACGCACCAAGATAACGTACAATg    >  2:438469/1‑151 (MQ=255)
                                                                                                                                       ttAGTCCGGCTCAAAATTAACGCGCGTTTAAAAATAGCACCGTATTGGGGCTCATCGGATTTGGCCTCGCTATTTTTACCCTTCAGGGTCGCGTAAAGAATTTTACTCCCCAGATAGAGCAAATAAAACGCACCAAGATAACGTACAATGt   >  2:346236/1‑151 (MQ=255)
                                                                                                                                        tAGTCCGGCTCAAAATTAACGCGCGTTTAAAAATAGCACCGTATTGGGGCTCATCGGATTTGGCCTCGCTATTTTTACCCTTCAGGGTCGCGTAAAGAATTTTACTCCCCAGATAGAGCAAATAAAACGCACCAAGATAACGTACAATGtt  >  1:114994/1‑151 (MQ=255)
                                                                                                                                             |                                                                                                                                                 
ATCAGGAAGCTCAAATAGCAGAAACTCACCAGTTCCAGCGTCGCCGCCAGAATAAAGAATGAAATTCCCGTATGTGGGGCATTAACATCGATAAACTGTACGAAAAACGACACATAGAACAAAATGGCTTTCGGATTAGTCAGGCTCAAAATTAACGCGCGTTTAAAAATAGCACCGTATTGGGGCTCATCGGATTTGGCCTCGCTATTTTTACCCTTCAGGGTCGCGTAAAGAATTTTACTCCCCAGATAGAGCAAATAAAACGCACCAAGATAACGTACAATGTT  >  NC_000913/1880245‑1880531

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: