Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,880,672	A→C	F30V (TTT→GTT)	leuE ←	leucine efflux protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,880,672	0	A	C	100.0%	204.4 / NA	54	F30V (TTT→GTT)	leuE	leucine efflux protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (23/31);  total (23/31)

ATGTTGAATAATATCGGGGTGGTCTTAATTAATGTCGCCACTCCAGCCCATGCCAGAAACATCAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGTACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATC  >  NC_000913/1880527‑1880819                                                                                                                                                  |                                                                                                                                                    aTGTTGAATAATATCGGGGTGGTCTTAATTAATGTCGCCACTCCAGCCCATGCCAGAAACATCAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGTACAACCAggg                                                                                                                                                >  2:356655/1‑151 (MQ=255) aTGTTGAATAATATCGGGGTGGTCTTAATTAATGTCGCCACTCCAGCCCATGCCAGAAACATCAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGTACAACCAggg                                                                                                                                                >  1:297181/1‑151 (MQ=255)     tGAATAATATCGGGGTGGTCTTAATTAATGTCGCCACTCCAGCCCATGCCAGAAACATCAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGTACAACCAGGGTAt                                                                                                                                             <  2:448821/150‑1 (MQ=255)      gAATAATATCGGGGTGGTCTTAATTAATGTCGCCACTCCAGCCCATGCCAGAAACATCAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGTACAACCAGGGTAttt                                                                                                                                           >  1:820806/1‑151 (MQ=255)        ataataTCGGGGTGGTCTTAATTAATGTCGCCACTCCAGCCCATGCCAGAAACATCAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGTACAACCAGGGTATTTgg                                                                                                                                         <  2:777067/151‑1 (MQ=255)         taataTCGGGGTGGTCTTAATTAATGTCGCCACTCCAGCCCATGCCAGAAACATCAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGTACAACCAGGGTATTTGGc                                                                                                                                        >  2:802707/1‑151 (MQ=255)               cGGGGTGGTCTTAATTAATGTCGCCACTCCAGCCCATGCCAGAAACATCAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGTACAACCAGGGTATTTGGCCCTGGc                                                                                                                                  <  1:632021/151‑1 (MQ=255)                ggggTGGTCTTAATTAATGTCGCCACTCCAGCCCATGCCAGAAACATCAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGTACAACCAGGGTATTTGGCCCTGGCa                                                                                                                                 <  2:277245/151‑1 (MQ=255)                     ggtCTTAATTAATGTCGCCACTCCAGCCCATGCCAGAAACATCAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGTACAACCAGGGTATTTGGCCCTGGCACCAac                                                                                                                            >  1:436879/1‑151 (MQ=255)                      gtCTTAATTAATGTCGCCACTCCAGCCCATGCCAGAAACATCAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGTACAACCAGGGTATTTGGCCCTGGCACCAaca                                                                                                                           <  2:928727/151‑1 (MQ=255)                      gtCTTAATTAATGTCGCCACTCCAGCCCATGCCAGAAACATCAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGTACAACCAGGGTATTTGGCCCTGGCACCAac                                                                                                                            >  2:349437/1‑150 (MQ=255)                         ttaattaaTGTCGCCACTCCAGCCCATGCCAGAAACATCAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGTACAACCAGGGTATTTGGCCCTGGCACCAAcaca                                                                                                                         >  1:406605/1‑150 (MQ=255)                              taatGTCGCCACTCCAGCCCATGCCAGAAACATCAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGTACAACCAGGGTATTTGGCCCTGGCACCAACACAATaaa                                                                                                                    <  1:859683/150‑1 (MQ=255)                               aatGTCGCCACTCCAGCCCATGCCAGAAACATCAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGTACAACCAGGGTATTTGGCCCTGGCACCAACACAATaaaa                                                                                                                   <  1:366287/150‑1 (MQ=255)                               aacgTCGCCACCCACGCCCATGCAAGAAACATCACTACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGTACAACCAGGGTATTTGGCCCTGGCACCAACACAAt                                                                                                                       >  1:437346/4‑146 (MQ=255)                                  gTCGCCACTCCAGCCCATGCCAGAAACATCAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGTACAACCAGGGTATTTGGCCCTGGCACCAACACAATAAAAATg                                                                                                                <  1:315464/150‑1 (MQ=255)                                              gCCCATGCCAGAAACATCAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGTACAACCAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAAcc                                                                                                       >  2:895630/1‑147 (MQ=255)                                                  aTGCCAGAAACATCAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGTACAACCAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGt                                                                                               >  1:282420/1‑151 (MQ=255)                                                     ccAGAAACATCAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGTACAACCAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCa                                                                                            <  2:114996/151‑1 (MQ=255)                                                        gAAACATCAATCCCGCATCGCCAATAAATCCACCGCAGGCCGCAAGATAACCGCCTTTCATCCCGCTACTGACGCTATTTTTGAGTACACCCAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCATTa                                                                                         <  1:98447/151‑1 (MQ=255)                                                         aaaCATCAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGTACAACCAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTa                                                                                         >  2:872107/1‑150 (MQ=255)                                                               cAATCCCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGTACAACCAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAg                                                                                   <  1:496627/150‑1 (MQ=255)                                                               cAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGTACAACCAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAg                                                                                   <  2:752591/150‑1 (MQ=255)                                                                   aCCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGTACAACCAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAAcc                                                                               <  1:812748/150‑1 (MQ=255)                                                                        aTCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGTACAACCAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTAt                                                                         <  2:338575/151‑1 (MQ=255)                                                                          cGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGTACAACCAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTAtt                                                                        <  1:24495/150‑1 (MQ=255)                                                                          cGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGTACAACCAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTc                                                                       >  1:376646/1‑151 (MQ=255)                                                                           gCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGTACAACCAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCa                                                                      <  2:376280/151‑1 (MQ=255)                                                                                taaataCACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGTACAACCAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGa                                                                  >  2:690040/1‑150 (MQ=255)                                                                                   ataCACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGTACAACCAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAacac                                                              >  1:912812/1‑151 (MQ=255)                                                                                          gCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGTACAACCAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAAcc                                                       >  2:829493/1‑151 (MQ=255)                                                                                            aGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGTACAACCAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACctc                                                     >  1:150080/1‑151 (MQ=255)                                                                                             ggCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGTACAACCAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACctct                                                    >  1:690689/1‑151 (MQ=255)                                                                                                    aGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGTACAACCAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTa                                             <  2:311916/151‑1 (MQ=255)                                                                                                    aGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGTACAACCAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTa                                             <  1:356642/151‑1 (MQ=255)                                                                                                        aaCCGCCTTTCATACCGCTACTGACGCTATTTTTGAGTACAACCAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCtt                                         >  2:299717/1‑151 (MQ=255)                                                                                                        aaCCGCCTTTCATACCGCTACTGACGCTATTTTTGAGTACAACCAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCtt                                         <  1:663818/151‑1 (MQ=255)                                                                                                          ccGCCTTTCATACCGCTACTGACGCTATTTTTGAGTACAACCAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTc                                       <  2:867688/151‑1 (MQ=255)                                                                                                           cGCCTTTCATACCGCTACTGACGCTATTTTTGAGTACAACCAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCg                                      <  1:23319/151‑1 (MQ=255)                                                                                                                ttCATACCGCTACTGACGCTATTTTTGAGTACAACCAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGATCa                                  <  1:759477/150‑1 (MQ=255)                                                                                                                 tCATACCGCTACTGACGCTATTTTTGAGTACAACCAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGATCATg                                >  1:700994/1‑151 (MQ=255)                                                                                                                    tACCGCTACTGACGCTATTTTTGAGTACAACCTGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTACAGTAATTCAGAACCCCGTATTCAGCGAACACATTACCCTCTTTAATTATCTTTCGATCAt                                 <  1:477437/147‑1 (MQ=255)                                                                                                                     aCCGCTACTGACGCTATTTTTGAGTACAACCAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGATCATGcgcg                            <  1:854500/151‑1 (MQ=255)                                                                                                                      ccGCTACTGACGCTATTTTTGAGTACAACCAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGATCATGcgcg                            >  2:280228/1‑150 (MQ=255)                                                                                                                          tACTGACGCTATTTTTGAGTACAACCAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGATCATGCGCGATTa                        >  2:810104/1‑150 (MQ=255)                                                                                                                           aCTGACGCTATTTTTGAGTACAACCAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGATCATGCGCGATTaa                       <  1:17726/150‑1 (MQ=255)                                                                                                                             tGACGCTATTTTTGAGTACAACCAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGATCATGCGCGATTAAAgg                    >  1:415824/1‑151 (MQ=255)                                                                                                                              gACGCTATTTTTGAGTACAACCAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGATCATGCGCGATTAAAgg                    <  1:438454/150‑1 (MQ=255)                                                                                                                              gACGCTATTTTTGAGTACAACCAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGATCATGCGCGATTAAAg                     <  1:299708/149‑1 (MQ=255)                                                                                                                                cGCTATTTTTGAGTACAACCAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGATCATGCGCGATTAAAGGTg                  <  2:626636/150‑1 (MQ=255)                                                                                                                                    aTTTTTGAGTACAACCAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGATCATGCGCGATTAAAGGTGAatat             <  2:428162/151‑1 (MQ=255)                                                                                                                                           aGTACAACCAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGATCATGCGCGATTAAAGGTGAATATGCTAAcc      <  2:935111/151‑1 (MQ=255)                                                                                                                                               cAACCAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATc  <  2:50277/151‑1 (MQ=255)                                                                                                                                               cAACCAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATc  >  1:920683/1‑151 (MQ=255)                                                                                                                                                  |                                                                                                                                                    ATGTTGAATAATATCGGGGTGGTCTTAATTAATGTCGCCACTCCAGCCCATGCCAGAAACATCAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGTACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATC  >  NC_000913/1880527‑1880819

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑