Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,924,236	A→C	L119R (CTG→CGG)	yebZ ←	inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,924,236	0	A	C	100.0%	208.0 / NA	57	L119R (CTG→CGG)	yebZ	inner membrane protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (27/30);  total (27/30)

CACCGGCAGTAATCCCCCAAACCACGCCGCCGCACAAATCAGGTGAATCGCGTGATTAGTCTGATGGATTTTCGCTGTTACCCCTTCATTCAGCGTCGCATGCCCCACTCCCGCCAGCAGAATAAATTGCGCGGTAGTGAGCATAAACAGCAAACGTGGCATATTCCGCGGTTGCATAAGGGCGACGATCAACGTTACGAGGGCGAGAACAATTTGCCATAACCAGATGCCACCAAACTGCGTTTGCAAAACAGCCTGCCAAATGTTTGGCGAAAATACATCTGTCCATCCCGT  >  NC_000913/1924088‑1924381                                                                                                                                                     |                                                                                                                                                  cACCGGCAGTAATCCCCCAAACCACGCCGCCGCACAAATCAGGTGAATCGCGTGATTAGTCTGATGGATTTTCGCTGTTACCCCTTCATTCAGCGTCGCATGCCCCACTCCCGCCAGCAGAATAAATTGCGCGGTAGTGAGCATAAACCGc                                                                                                                                                 <  1:724637/151‑1 (MQ=255)   ccGGCAGTAATCCCCCAAACCACGCCGCCGCACAAATCAGGTGAATCGCGTGATTAGTCTGATGGATTTTCGCTGTTACCCCTTCATTCAGCGTCGCATGCCCCACTCCCGCCAGCAGAATAAATTGCGCGGTAGTGAGCATAAACCGCa                                                                                                                                                <  1:371894/150‑1 (MQ=255)         gTAATCCCCCAAACCACGCCGCCGCACAAATCAGGTGAATCGCGTGATTAGTCTGATGGATTTTCGCTGTTACCCCTTCATTCAGCGTCGCATGCCCCACTCCCGCCAGCAGAATAAATTGCGCGGTAGTGAGCATAAACCGCAAACGTgg                                                                                                                                         <  1:863556/151‑1 (MQ=255)                              ccgcACAAATCAGGTGAATCGCGTGATTAGTCTGATGGATTTTCGCTGTTACCCCTTCGTTCAGCGTCGCATGCCCCACTCCCGCCAGCAGAATAAATTGCGCGGTAGTGAGCATAAACCGCAAACGTGGCATATTCCGCGGTTGCATAAg                                                                                                                    <  2:651792/151‑1 (MQ=255)                              ccgcACAAATCAGGTGAATCGCGTGATTAGTCTGATGGATTTTCGCTGTTACCCCTTCATTCAGCGTCGCATGCCCCCCTCCCGCCAGCAGAATAAATTGCGCGGTAGTGAGCATAAACCGCAAACGTGGCATATTCCGCGGTTGCATaa                                                                                                                     <  2:752854/150‑1 (MQ=255)                                  acaAATCAGGTGAATCGCGTGATTAGTCTGATGGATTTTCGCTGTTACCCCTTCATTCAGCGTCGCATGCCCCACTCCCGCCAGCAGAATAAATTGCGCGGTAGTGAGCATAAACCGCAAACGTGGCATATTCCGCGGTTGCATAAGGGc                                                                                                                 <  2:581822/150‑1 (MQ=255)                                      aTCAGGTGAATCGCGTGATTAGTCTGATGGATTTTCGCTGTTACCCCTTCATTCAGCGTCGCATGCCCCACTCCCGCCAGCAGAATAAATTGCGCGGTAGTGAGCATAAACCGCAAACGTGGCATATTCCGCGGTTGCATAAGGGcgacg                                                                                                             >  1:45829/1‑150 (MQ=255)                                        cAGGTGAATCGCGTGATTAGTCTGATGGATTTTCGCTGTTACCCCTTCATTCAGCGTCGCATGCCCCACTCCCGCCAGCAGAATAAATTGCGCGGTAGTGAGCATAAACCGCAAACGTGGCATATTCCGCGGTTGCATAAGGGCGACGATc                                                                                                          >  2:220658/1‑151 (MQ=255)                                         aGGTGAATCGCGTGATTAGTCTGATGGATTTTCGCTGTTACCCCTTAATTCAGCGTCGCATGCCCCACTCCCGCCAGCAGAATAAATTGCGCGGTAGTGAGCATAAACCGCAAACGTGGCATATTCCGCGGTTGCATAAGGGCGACGATc                                                                                                          >  1:481740/1‑150 (MQ=255)                                                    gTGATTAGTCTGATGGATTTTCGCTGTTACCCCTTCATTCAGCGTCGCATGCCCCACTCCCGCCAGCAGAATAAATTGCGCGGTAGTGAGCATAAACCGCAAACGTGGCATATTCCGCGGTTGCATAAGGGCGACGATCAACGTTACGAgg                                                                                              <  1:550658/151‑1 (MQ=255)                                                     tGATTAGTCTGATGGATTTTCGCTGTTACCCCTTCATTCAGCGTCGCATGCCCCACTCCCGCCAGCAGAATAAATTGCGCGGTAGTGAGCATAAACCGCAAACGTGGCATATTCCGCGGTTGCATAAGGGCGACGATCAACGTTACGAgg                                                                                              <  2:248116/150‑1 (MQ=255)                                                                       ttCGCTGTTACCCCTTCATTCAGCGTCGCATGCCCCACTCCCGCCAGCAGAATAAATTGCGCGGTAGTGAGCATAAACCGCAAACGTGGCATATTCCGCGGTTGCATAAGGGCGACGATCAACGTTACGAGGGCGAGAACAATTTGCCAt                                                                            >  2:178685/1‑150 (MQ=255)                                                                         cGCTGTTACCCCTTCATTCAGCGTCGCATGCCCCACTCCCGCCAGCAGAATAAATTGCGCGGTAGTGAGCATAAACCGCAAACGTGGCATATTCCGCGGTTGCATAAGGGCGACGATCAACGTTACGAGGGCGAGAACAATTTGCCATAAc                                                                         >  1:643375/1‑151 (MQ=255)                                                                          gttGTTCCCCCTCCATTCAGCCTCGCTCGCCCCACTCCCGCCAGCAGAAAAAATTGCGCGGTAGTGAGCATAAACCGCAAACGTGGCATATTCCGCGGTTGCATAAGGGCGACGATCAACGTTACGAGGGCGAGAACAATTTGCCAt                                                                            <  1:579256/145‑1 (MQ=255)                                                                          gCTGTTACCCCTTCATTCAGCGTCGCATGCCCCACTCCCGCCAGCAGAATAAATTGCGCGGTAGTGAGCATAAACCGCAAACGTGGCATATTCCGCGGTTGCATAAGGGCGACGATCAACGTTACGAGGGCGAGAACAATTTGCCATAAcc                                                                        >  1:800971/1‑151 (MQ=255)                                                                             gTTACCCCTTCATTCAGCGTCGCATGCCCCACTCCCACCAGCAGAATAAATTGCGCGGTAGTGAGCATAAACCGCAAACGTGGCATATTCCGCGGTTGCATAAGGGCGACGATCAACGTTACGAGGGCGAGAACAATTTGCCATAACCAGa                                                                     <  2:601714/151‑1 (MQ=255)                                                                               tACCCCTTCATTCAGCGTCGCATGCCCCACTCCCGCCAGCAGAATAAATTGCGCGGTAGTGAGCATAAACCGCAAACGTGGCATATTCCGCGGTTGCATAAGGGCGACGATCAACGTTACGAGGGCGAGAACAATTTGCCATAACCAGATg                                                                   >  1:406007/1‑151 (MQ=255)                                                                               tACCCCTTCATTCAGCGTCGCATGCCCCACTCCCGCCAGCAGAATAAATTGCGCGGTAGTGAGCATAAACCGCAAACGTGGCATATTCCGCGGTTGCATAAGGGCGACGATCAACGTTACGAGGGCGAGAACAATTTGCCATAACCAGATg                                                                   >  2:162090/1‑151 (MQ=255)                                                                                 ccccTTCATCCAGCGTCGCATGCCCCACTCCCGCCAGCAGAATAAATTGCGCGGTAGTGAGCATAAACCGCAAACGTGGCATATTCCGCGGTTGCATAAGGGCGACGATCAACGTTACGAGGGCGAGAACAATTTGCCATAACCAGATg                                                                   <  2:247028/149‑1 (MQ=255)                                                                                  cccTTCATTCAGCGTCGCATGCCCCACTCCCGCCAGCAGAATAAATTGCGCGGTAGTGAGCATAAACCGCAAACGTGGCATATTCCGCGGTTGCATAAGGGCGACGATCAACGTTACGAGGGCGAGAACAATTTGCCATAACCAGATGcc                                                                 >  2:560898/1‑150 (MQ=255)                                                                                       cattcaGCGTCGCATGCCCCACTCCCGCCAGCAGAATAAATTGCGCGGTAGTGAGCATAAACCGCAAACGTGGCATATTCCGCGGTTGCATAAGGGCGACGATCAACGTTACGAGGGCGAGAACAATTTGCCATAACCAGATGCCACCaa                                                            >  2:855484/1‑150 (MQ=255)                                                                                       cattcaGCGTCGCATGCCCCACTCCCGCCAGCAGAATAAATTGCGCGGTAGTGAGCATAAACCGCAAACGTGGCATATTCCGCGGTTGCATAAGGGCGACGATCAACGTTACGAGGGCGAGAACAATTTGCCATAACCAGATGCCACCaa                                                            <  1:408525/150‑1 (MQ=255)                                                                                       cattcaGCGTCGCATGCCCCACTCCCGCCAGCAGAATAAATTGCGCGGCAGTGAGCATAAACCGCAAACGTGGCATATTCCGCGGTTGCATAAGGGCGACGATCAACGTTACGAGGGCGAGAACAATTTGCCATAACCAGATGCCACCaa                                                            >  2:339819/1‑150 (MQ=255)                                                                                        attcaGCGTCGCATGCCCCACTCCCGCCAGCAGAATAAATTGCGCGGTAGTGAGCATAAACCGCAAACGTGGCATATTCCGCGGTTGCATAAGGGCGACGATCAACGTTACGAGGGCGAGAACAATTTGCCATAACCAGATGccacca                                                             <  1:696963/148‑1 (MQ=255)                                                                                            aGCGTCGCATGCCCCACTCCCGCCAGCAGAATAAATTGCGCGGTAGTGAGCATAAACCGCAAACGTGGCATATTCCGCGGTTGCATAAGGGCGACGATCAACGTTACGAGGGCGAGAACAATTTGCCATAACCAGATGCCACCAAACTGCg                                                      >  2:872206/1‑151 (MQ=255)                                                                                            aGCGTCGCATGCCCCACCCCCGCCAGCAGAATAAATTGCGCGGTAGTGAGCATAAACCGCAAACGTGGCATATTCCGCGGTTGCATAAGGGCGACGATCAACGTTACGAGGGCGAGAACAATTTGCCATAACCAGATGCCACCAAACt                                                         <  2:48978/148‑1 (MQ=255)                                                                                              cGTCGCATGCCCCACTCCCGCCAGCAGAATAAATTGCGCGGTAGTGAGCATAAACCGCAAACGTGGCATATTCCGCGGTTGCATAAGGGCGACGATCAACGTTACGAGGGCGAGAACAATTTGCCATAACCAGATGCCACCAAACTGCg                                                      <  1:516725/149‑1 (MQ=255)                                                                                               gTCGCATGCCCCACTCCCGCCAGCAGAATAAATTGCGCGGTAGTGAGCATAAACCGCAAACGTGGCATATTCCGCGGTTGCATAAGGGCGACGATCAACGTTACGAGGGCGAGAACAATTTGCCATAACCAGATGCCACCAAACTGCGttt                                                   >  1:139981/1‑151 (MQ=255)                                                                                                   cATGCCCCACTCCCGCCAGCAGAATAAATTGCGCGGTAGTGAGCATAAACCGCAAACGTGGCATATTCCGCGGTTGCATAAGGGCGACGATCAACGTTACGAGGGCGAGAACAATTTGCCATAACCAGATGCCACCAAACTGCGTTTGCaa                                               <  1:560604/151‑1 (MQ=255)                                                                                                   cATGCCCCACTCCCGCCAGCAGAATAAATTGCGCGGTAGTGAGCATAAACCGCAAACGTGGCATATTCCGCGGTTGCATAAGGGCGACGATCAACGTTACGAGGGCGAGAACAATTTGCCATAACCAGATGCCACCAAACTGCGTTTGCa                                                >  2:412108/1‑150 (MQ=255)                                                                                                     tGCCCCACTCCCGCCAGCAGAATAAATTGCGCGGTAGTGAGCATAAACCGCAAACGTGGCATATTCCGCGGTTGCATAAGGGCGACGATCAACGTTACGAGGGCGAGAACAATTTGCCATAACCAGATGCCACCAAACTGCGTTTGCaaa                                              >  2:317587/1‑150 (MQ=255)                                                                                                       ccccACTCCCGCCAGCAGAATAAATTGCGCGGTAGTGAGCATAAACCGCAAACGTGGCATATTCCGCGGTTGCATAAGGGCGACGATCAACGTTACGAGGGCGAGAACAATTTGCCATAACCAGATGCCACCAAACTGCGTTTGCaaaa                                             <  1:166835/149‑1 (MQ=255)                                                                                                            cTCCCGCCAGCAGAATAAATTGCGCGGTAGTGAGCATAAACCGCAAACGTGGCATATTCCGCGGTTGCATAAGGGCGACGATCAACGTTACGAGGGCGAGAACAATTTGCCATAACCAGATGCCACCAAACTGCGTTTGCAAAACAGCCt                                       <  1:461568/150‑1 (MQ=255)                                                                                                            cTCCCGCCAGCAGAATAAATTGCGCGGTAGTGAGCATAAACCGCAAACGTGGCATATTCCGCGGTTGCATAAGGGCGACGATCAACGTTACGAGGGCGAGAACAATTTGCCATAACCAGATGCCACCAAACTGCGTTTGCAAAACAGCCTg                                      >  1:484035/1‑151 (MQ=255)                                                                                                             tCCCGCCAGCAGAATAAATTGCGCGGTAGTGAGCATAAACCGCAAACGTGGCATATTCCGCGGTTGCATAAGGGCGACGATCAACGTTACGAGGGCGAGAACAATTTGCCATAACCAGATGCCACCAAACTGCGTTTGCAAAACAGCCTGc                                     >  2:732077/1‑151 (MQ=255)                                                                                                             tCCCGCCAGCAGAATAAATTGCGCGGTAGTGAGCATAAACCGCAAACGTGGCATATTCCGCGGTTGCATAAGGGCGACGATCAACGTTACGAGGGCGAGAACAATTTGCCATAACCAGATGCCACCAAACTGCGTTTGCAAAACAGCCTGc                                     >  1:537039/1‑151 (MQ=255)                                                                                                                cGCCAGCAGAATAAATTGCGCGGTAGTGAGCATAAACCGCAAACGTGGCATATTCCGCGGTTGCATAAGGGCGACGATCAACGTTACGAGGGCGAGAACAATTTGCCATAACCAGATGCCACCAAACTGCGTTTGCAAAACAGCCTGCCaa                                  <  2:654653/151‑1 (MQ=255)                                                                                                                 gCCAGCAGAATAAATTGCGCGGTAGTGAGCATAAACCGCAAACGTGGCATATTCCGCGGTTGCATAAGGGCGACGATCAACGTTACGAGGGCGAGAACAATTTGCCATAACCAGATGCCACCAAACTGCGTTTGCAAAACAGCCTGCCaaa                                 <  2:618/151‑1 (MQ=255)                                                                                                                  ccAGCAGAATAAATTGCGCGGTAGTGAGCATAAACCGCAAACGTGGCATATTCCGCGGTTGCATAAGGGCGACGATCAACGTTACGAGGGCGAGAACAATTTGCCATAACCAGATGCCACCAAACTGCGTTTGCAAAACAGCCTGCCAAAt                                <  2:825422/151‑1 (MQ=255)                                                                                                                   cagcagAATAAATTGCGCGGTAGTGAGCATAAACCGCAAACGTGGCATATTCCGCGGTTGCATAAGGGCGACGATCAACGTTACGAGGGCGAGAACAATTTGCCATAACCAGATGCCACCAAACTGCGTTTGCAAAACAGCCTGCCAAATg                               >  1:818962/1‑151 (MQ=255)                                                                                                                      cagAATAAATTGCGCGGTAGTGAGCATAAACCGCAAACGTGGCATATTCCGCGGTTGCATAAGGGCGACGATCAACGTTACGAGGGCGAGAACAATTTGCCATAACCAGATGCCGCCAAACTGCGTTTTCAAAACCGCCTGCCAAATGt                              <  1:29017/149‑1 (MQ=255)                                                                                                                      cagAATAAATTGCGCGGTAGTGAGCATAAACCGCAAACGTGGCATATTCCGCGGTTGCATAAGGGCGACGATCAACGTTACGAGGGCGAGAACAATTTGCCATAACCAGATGCCACCAAACTGCGTTTGCAAAACAGCCTGCCAAATGttt                            <  2:881625/151‑1 (MQ=255)                                                                                                                      cagAATAAATTGCGCGGTAGTGAGCATAAACCGCAAACGTGGCATATTCCGCGGTTGCATAAGGGCGACGATCAACGTTACGAGGGCGAGAACAATTTGCCATAACCAGATGCCACCAAACTGCGTTTGCAAAACAGCCTGCCAAATGttt                            <  1:124943/151‑1 (MQ=255)                                                                                                                       agAATAAATTGCGCGGTAGTGAGCATAAACCGCAAACGTGGCATATTCCGCGGTTGCATAAGGGCGACGATCAACGTTACGAGGGCGAGAACAATTTGCCATAACCAGATGCCACCAAACTGCGTTTGCAAAACAGCCTGCCAAATGTTTg                           >  2:714428/1‑151 (MQ=255)                                                                                                                       agAATAAATTGCGCGGTAGTGAGCATAAACCGCAAACGTGGCATATTCCGCGGTTGCATAAGGGCGACGATCAACGTTACGAGGGCGAGAACAATTTGCCATAACCAGATGCCACCAAACTGCGTTTGCAAAACAGCCTGCCAAATGTTTg                           <  1:878560/151‑1 (MQ=255)                                                                                                                        gAATAAATTGCGCGGTAGTGAGCATAAACCGCAAACGTGGCATATTCCGCGGTTGCATAAGGGCGACGATCAACGTTACGAGGGCGAGAACAATTTGCCATAACCAGATGCCACCAAACTGCGTTTGCAAAACAGCCTGCCAAATGTTTg                           <  2:387437/150‑1 (MQ=255)                                                                                                                             aaTTGCGCGGTAGTGATCATAAACCGCAAACGTGGCATATTCCGCGGTTGCATAAGGGCGACGATCAACGTTACGAGGGCGAGAACAATTTGCCATAACCAGATGCCACCAAACTGCGTTTGCAAAACAGCCTGCCAAATGTTTGGCGaaa                     <  1:51947/151‑1 (MQ=255)                                                                                                                                tGCGCGGTAGTGAGCATAAACCGCAAACGTGGCATATTCCGCGGTTGCATAAGGGCGACGATCAACGTTACGAGGGCGAGAACAATTTGCCATAACCAGATGCCACCAAACTGCGTTTGCAAAACAGCCTGCCAAATGTTTGGCGAAAAt                   <  2:525730/150‑1 (MQ=255)                                                                                                                                tGCGCGGTAGTGAGCATAAACCGCAAACGTGGCATATTCCGCGGTTGCATAAGGGCGACGATCAACGTTACGAGGGCGAGAACAATTTGCCATAACCAGATGCCACCAAACTGCGTTTGCAAAACAGCCTGCCAAATGTTTGGCGAAAAt                   <  2:277327/150‑1 (MQ=255)                                                                                                                                tGCGCGGTAGTGAGCATAAACCGCAAACGTGGCATATTCCGCGGTTGCATAAGGGCGACGATCAACGTTACGAGGGCGAGAACAATTTGCCATAACCAGATGCCACCAAACTGCGTTTGCAAAACAGCCTGCCAAATGTTTGGCGAAAAt                   <  1:655115/150‑1 (MQ=255)                                                                                                                                  cgcgGTAGTGAGCATAAACCGCAAACGTGGCATATTCCGCGGTTGCATAAGGGCGACGATCAACGTTACGAGGGCGAGAACAATTTGCCATAACCAGATGCCACCAAACTGCGTTTGCAAAACAGCCTGCCAAATGTTTGGCGAAAATAc                 >  2:497476/1‑150 (MQ=255)                                                                                                                                    cgGTAGTGAGCATAAACCGCAAACGTGGCATATTCCGCGGTTGCATAAGGGCGACGATCAACGTTACGAGGGCGAGAACAATTTGCCATAACCAGATGCCACCAAACTGCGTTTGCAAAACAGCCTGCCAAATGTTTGGCGAAAATACATc              >  2:859692/1‑151 (MQ=255)                                                                                                                                     ggTAGTGAGCATAAACCGCAAACGTGGCATATTCCGCGGTTGCATAAGGGCGACGATCAACGTTACGAGGGCGAGAACAATTTGCCATAACCAGATGCCACCAAACTGCGTTTGCAAAACAGCCTGCCAAATGTTTGGCGAAAATACATCt             >  1:728149/1‑151 (MQ=255)                                                                                                                                     ggTAGTGAGCATAAACCGCAAACGTGGCATATTCCGCGGTTGCATAAGGGCGACGATCAACGTTACGAGGGCGAGAACAATTTGCCATAACCAGATGCCACCAAACTGCGTTTGCAAAACAGCCTGCCAAATGTTTGGCGAAAATACATCt             >  1:807572/1‑151 (MQ=255)                                                                                                                                       tAGTGAGCATAAACCGCAAACGTGGCATATTCCGCGGTTGCATAAGGGCGACGATCAACGTTACGAGGGCGAGAACAATTTGCCATAACCAGATGCCACCAAACTGCGTTTGCAAAACAGCCTGCCAAATGTTTGGCGAAAATACATCTGt           >  1:186499/1‑151 (MQ=255)                                                                                                                                          tGAGCATAAACCGCAAACGTGGCATATTCCGCGGTTGCATAAGGGCGACGATCAACGTTACGAGGGCGAGAACAATTTGCCATAACCAGATGCCACCAAACTGCGTTTGCAAAACAGCCTGCCAAATGTTTGGCGAAAATACATCTGTcc         >  2:396380/1‑150 (MQ=255)                                                                                                                                                tAAACCGCAAACGTGGCATATTCCGCGGTTGCATAAGGGCGACGATCAACGTTACGAGGGCGAGAACAATTTGCCATAACCAGATGCCACCAAACTGCGTTTGCAAAACAGCCTGCCAAATGTTTGGCGAAAATACATCTGTCCATCCCGt  >  2:493951/1‑151 (MQ=255)                                                                                                                                                     |                                                                                                                                                  CACCGGCAGTAATCCCCCAAACCACGCCGCCGCACAAATCAGGTGAATCGCGTGATTAGTCTGATGGATTTTCGCTGTTACCCCTTCATTCAGCGTCGCATGCCCCACTCCCGCCAGCAGAATAAATTGCGCGGTAGTGAGCATAAACAGCAAACGTGGCATATTCCGCGGTTGCATAAGGGCGACGATCAACGTTACGAGGGCGAGAACAATTTGCCATAACCAGATGCCACCAAACTGCGTTTGCAAAACAGCCTGCCAAATGTTTGGCGAAAATACATCTGTCCATCCCGT  >  NC_000913/1924088‑1924381

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑