Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,980,663	T→G	E317A (GAA→GCA)	otsA ←	trehalose‑6‑phosphate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,980,663	0	T	G	100.0%	191.5 / NA	50	E317A (GAA→GCA)	otsA	trehalose‑6‑phosphate synthase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (25/25);  total (25/25)

ATCCCGTCACGCAGTGGCGTCACTAAGCCCACGTCAGAGTAGCGGAATATTTTCATCAGTAATTTACGGTCAAAATGCTGATTCAAATAATAAAGCGGCGTCCAGCCTAATTGCCCGTATTTACCATTAATTCGTCCAGCTTCATTTTCGAGCTGATGACGAATATCCTGATAGGCTTGCACATCACCACGCGACGTTGGTGCAATCTGGGTATAACGAATTTTACCATGATGCTGCGGATATTTTTCCAGCAACGCTTCATAGGCGAGAAAACGCTCTGGCAAACC  >  NC_000913/1980522‑1980808                                                                                                                                              |                                                                                                                                                  aTCCCGTCACGCAGTGGCGTCACTAAGCCCACGTCAGAGTAGCGGAATATTTTCATCAGTAATTTACGGTCAAAATGCTGATTCAAATAATAAAGCGGCGTCCAGCCTAATTGCCCGTATTTACCATTAATTCGTCCAGCTGCATTTTCGa                                                                                                                                          <  1:122347/151‑1 (MQ=255)   cccGTCACGCAGTGGCGTCACTAAGCCCACGTCAGAGTAGCGGAATATTTTCATCAGTAATTTACGGTCAAAATGCTGATTCAAATAATAAAGCGGCGTCCAGCCTAATTGCCCGTATTTACCATTAATTCGTCCAGCTGCATTTTCGAg                                                                                                                                         <  2:103409/150‑1 (MQ=255)   cccGTCACGCAGTGGCGTCACTAAGCCCACGTCAGAGTAGCGGAATATTTTCATCAGTAATTTACGGTCAAAATGCTGATTCAAATAATAAAGCGGCGTCCAGCCTAATTGCCCGTATTTACCATTAATTCGTCCAGCTGCATTTTCGAg                                                                                                                                         <  2:96382/150‑1 (MQ=255)           gCAGTGGCGTCACTAAGCCCACGTCAGAGTAGCGGAATATTTTCATCAGTAATTTACGGTCAAAATGCTGATTCAAATAATAAAGCGGCGTCCAGCCTAATTGCCCGTATTTACCATTAATTCGTCCAGCTGCATTTTCGAGCTGATGACg                                                                                                                                >  2:398915/1‑151 (MQ=255)            cAGTGGCGTCACTAAGCCCACGTCAGAGTAGCGGAATATTTTCATCAGTAATTTACGGTCAAAATGCTGATTCAAATAATAAAGCGGCGTCCAGCCTAATTGCCCGTATTTACCATTAATTCGTCCAGCTGCATTTTCGAGCTGATGACGa                                                                                                                               <  2:107923/151‑1 (MQ=255)             aGTGGCGTCACTAAGCCCACGTCAGAGTAGCGGAATATTTTCATCAGTAATTTACGGTCAAAATGCTGATTCAAATAATAAAGCGGCGTCCAGCCTAATTGCCCGTATTTACCATTAATTCGTCCAGCTGCATTTTCGAGCTGATGACGaa                                                                                                                              <  1:135826/151‑1 (MQ=255)             aGTGGCGTCACTAAGCCCACGTCAGAGTAGCGGAATATTTTCATCAGTAATTTACGGTCAAAATGCTGATTCAAATAATAAAGCGGCGTCCAGCCTAATTGCCCGTATTTACCATTAATTCGTCCAGCTGCATTTTCGAGCTGATGACGa                                                                                                                               >  1:766320/1‑150 (MQ=255)              gTGGCGTCACTAAGCCCACGTCAGAGTAGCGGAATATTTTCATCAGTAATTTACGGTCAAAATGCTGATTCAAATAATAAAGCGGCGTCCAGCCTAATTGCCCGTATTTACCATTAATTCGTCCAGCTGCATTTTCGAGCTGATGACGaa                                                                                                                              >  2:27415/1‑150 (MQ=255)                   gTCACTAAGCCCACGTCAGAGTAGCGGAATATTTTCATCAGTAATTTACGGTCAAAATGCTGATTCAAATAATAAAGCGGCGTCCAGCCTAATTGCCCGTATTTACCATTAATTCGTCCAGCTGCATTTTCGAGCTGATGACGAATATCCt                                                                                                                        <  1:522755/151‑1 (MQ=255)                     cACTAAGCCCACGTCAGAGTAGCTGAATATTTTCATCAGTAATTTACGGTCAAAATGCTGATTCAAATAATAAAGCTGCGTCCAGCCTAATTGCCCGTAGTTACCAATAATGCGTCCAGCTGCATTTTCGAGCTGATGACGAATATCCTg                                                                                                                       <  2:921837/150‑1 (MQ=255)                      aCTAAGCCCACGTCAGAGTAGCGGAATATTTTCATCAGTAATTTACGGTCAAAATGCTGATTCAAATAATAAAGCGGCGTCCAGCCTAATTGCCCGTATTTACCATTAATTCGTCCAGCTGCATTTTCGAGCTGATGACGAATATCCTGAt                                                                                                                     >  1:586922/1‑151 (MQ=255)                        tAAGCCCACGTCAGAGTAGCGGAATATTTTCATCAGTAATTTACGGTCAAAATGCTGATTCAAATAATAAAGCGGCGTCCAGCCTAATTGCCCGTATTTACCATTAATTCGTCCAGCTGCATTTTCGAGCTGATGACGAATATCCTGAt                                                                                                                     >  1:415810/1‑149 (MQ=255)                           gCCCACGTCAGAGTAGCGGAATATTTTCATCAGTAATTTACGGTCAAAATGCTGATTCAAATAATAAAGCGGCGTCCAGCCTAATTGCCCGTATTTACCATTAATTCGTCCAGCTGCATTTTCGAGGTGATGACGAATATCCTGATAGGCt                                                                                                                <  2:695421/151‑1 (MQ=255)                            cccACGTCAGAGTAGCGGAATATTTTCATCAGTAATTTACGGTCAAAATGCTGATTCAAATAATAAAGCGGCGTCCAGCCTAATTGCCCGTATTTACCATTAATTCGTCCAGCTGCATTTTCGAGCTGATGACGAATATCCTGATAGGCtt                                                                                                               >  2:703639/1‑151 (MQ=255)                             ccACGTCAGAGTAGCGGAATATTTTCATCAGTAATTTACGGTCAAAATGCTGATTCAAATAATAAAGCGGCGTCCAGCCTAATTGCCCGTATTTACCATTAATTCGTCCAGCTGCATTTTCGAGCTGATGACGAATATCCTGATAGGCTTg                                                                                                              <  1:343812/151‑1 (MQ=255)                                     gagTAGCGGAATATTTTCATCAGTAATTTACGGTCAAAATGCTGATTCAAATAATAAAGCGGCGTCCAGCCTAATTGCCCGTATTTACCATTAATTCGTCCAGCTGCATTTTCGAGCTGATGACGAATATCCTGATAGGCTTGCACATcac                                                                                                      <  2:871313/151‑1 (MQ=255)                                         aGCGGAATATTTTCATCAGTAATTTACGGTCAAAATGCTGATTCAAATAATAAAGCGGCGTCCAGCCTAATTGCCCGTATTTACCATTAATTCGTCCAGCTGCATTTTCGAGCTGATGACGAATATCCTGATAGGCTTGCACATcaccac                                                                                                   <  1:770682/150‑1 (MQ=255)                                         aGCGGAATATTTTCATCAGTAATTTACGGTCAAAATGCTGATTCAAATAATAAAGCGGCGTCCAGCCTAATTGCCCGTATTTACCATTAATTCGTCCAGCTGCATTTTCGAGCTGATGACGAATATCCTGATAGGCTTGCACATCACCAcg                                                                                                  >  1:540676/1‑151 (MQ=255)                                                  ttttCATCAGTAATTTACGGTCAAAATGCTGATTCAAATAATAAAGCGGCGTCCAGCCTAATTGCCCGTATTTACCATTAATTCGTCCAGCTGCATTTTCGAGCTGATGACGAATATCCTGATAGGCTTGCACATCACCACGCGACGTTgg                                                                                         >  2:28650/1‑151 (MQ=255)                                                   tttCATCAGTAATTTACGGTCAAAATGCTGATTCAAATAATAAAGCGGCGTCCAGCCTAATTGCCCGTATTTACCATTAATTCGTCCAGCTGCATTTTCGAGCTGATGACGAATATCCTGATAGGCTTGCACATCACCACGCGACGTTgg                                                                                         >  1:537621/1‑150 (MQ=255)                                                     tcatcaGTAATTTACGGTCAAAATGCTGATTCAAATAATAAAGCGGCGTCCAGCCTAATTGCCCGTATTTACCATTAATTCGTCCAGCTGCATTTTCGAGCTGATGACGAATATCCTGATAGGCTTGCACATCACCACGCGACGTTGGTg                                                                                       <  1:181983/150‑1 (MQ=255)                                                      catcaGTAATTTACGGTCAAAATGCTGATTCAAATAATAAAGCGGCGTCCAGCCTAATTGCCCGTATTTACCATTAATTCGTCCAGCTGCATTTTCGAGCTGATGACGAATATCCTGATAGGCTTGCACATCACCACGCGACGTTGGTGc                                                                                      >  1:33845/1‑150 (MQ=255)                                                             aaTTTACGGTCAAAATGCTGATTCAAATAATAAAGCGGCGTCCAGCCTAATTGCCCGTATTTACCATTAATTCGTCCAGCTGCATTTTCGAGCTGATGACGAATATCCTGATAGGCTTGCACATCACCACGCGACGTTGGTGCAATCTggg                                                                              >  1:182205/1‑151 (MQ=255)                                                             aaTTTACGGTCAAAATGCTGATTCAAATAATAAAGCGGCGTCCAGCCTAATTGCCCGTATTTACCATTAATTCGTCCAGCTGCATTTTCGAGCTGATGACGAATATCCTGATAGGCTTGCACATCACCACGCGACGTTGGTGCAATCTggg                                                                              >  2:610006/1‑151 (MQ=255)                                                             aaTTTACGGTCAAAATGCTGATTCAAATAATAAAGCGGCGTCCAGCCTAATTGCCCGTATTTACCATTAATTCGTCCAGCTGCATTTTCGAGCTGATGACGAATATCCTGATAGGCTTGCACATCACCACGCGACGTTGGTGCAATCTggg                                                                              >  2:788536/1‑151 (MQ=255)                                                                 tACGGTCAAAATGCTGATTCAAATAATAAAGCGGCGTCCAGCCTAATTGCCCGTATTTACCATTAATTCGTCAAGCTGCCTTTTCGAGCTGATGACGAATATCCTGATAGGCTTGCACATCACCACGCGACGTTGGTGCAATCTGGGtat                                                                           >  1:125241/1‑150 (MQ=255)                                                                        aaaaTGCTGATTCAAATAATAAAGCGGCGTCCAGCCTAATTGCCCGTATTTACCATTAATTCGTCCAGCTGCATTTTCGAGCTGATGACGAATATCCTGATAGGCTTGCACATCACCACGCGACGTTGGTGCAATCTGGGTATAACGAAtt                                                                   >  2:919854/1‑151 (MQ=255)                                                                         aaaTGCTGATTCAAATAATAAAGCGGCGTCCAGCCTAATTGCCCGTATTTACCATTAATTCGTCCAGCTGCATTTTCGAGCTGATGACGAATATCCTGATAGGCTTGCACATCACCACGCGACGTTGGTGCAATCTGGGTATAACGAAttt                                                                  <  1:804604/151‑1 (MQ=255)                                                                              cTGATTCAAATAATAAAGCGGCGTCCAGCCTAATTGCCCGTATTTACCATTAATTCGTCCAGCTGCATTTTCGAGCTGATGACGAATATCCTGATAGGCTTGCACATCACCACGCGACGTTGGTGCAATCTGGGTATAACGAATTTTAcc                                                              >  1:533139/1‑150 (MQ=255)                                                                                 aTTCAAATAATAAAGCGGCGTCCAGCCTAATTGCCCGTATTTACCATTAATTCGTCCAGCTGCATTTTCGAGCTGATGACGAATATCCTGATAGGCTTGCACATCACCACGCGACGTTGGTGCAATCTGGGTATAACGAATTTTACCatg                                                           >  1:8538/1‑150 (MQ=255)                                                                                   tCAAATAATAAAGCGGCGTCCAGCCTAATTGCCCGTATTTACCATTAATTCGTCCAGCTGCATTTTCGAGCTGATGACGAATATCCTGATAGGCTTGCACATCACCACGCGACGTTGGTGCAATCTGGGTATAACGAATTTTACCatgat                                                         <  1:325073/150‑1 (MQ=255)                                                                                            aaaGCGGCGTCCAGCCTAATTGCCCGTATTTACCATTAATTCGTCCAGCTGCATTTTCGAGCTGATGACGAATATCCTGATAGGCTTGCACATCACCACGCGACGTTGGTGCAATCTGGGTATAACGAATTTTACCATGATGCTGCGGata                                               <  2:443928/151‑1 (MQ=255)                                                                                                cggcgTCCAGCCTAATTGCCCGTATTTACCATTAATTCGTCCAGCTGCATTTTCGAGCTGATGACGAATATCCTGATAGGCTTGCACATCACCACGCGACGTTGGTGCAATCTGGGTATAACGAATTTTACCATGATGCTGCGGATAtttt                                           >  2:621403/1‑151 (MQ=255)                                                                                                          ccTAATTGCCCGTATTTACCATTAATTCGTCCAGCTGCATTTTCGAGCTGATGACGAATATCCTGATAGGCTTGCACATCACCACGCGACGTTGGTGCAATCTGGGTATAACGAATTTTACCATGATGCTGCGGATATTTTTCCAGCAACg                                 <  1:762483/151‑1 (MQ=255)                                                                                                             aaTTGCCCGTATTTACCATTAATTCGTCCAGCTGCATTTTCGAGCTGATGACGAATATCCTGATAGGCTTGCACATCACCACGCGACGTTGGTGCAATCTGGGTATAACGAATTTTACCATGATGCTGCGGATATTTTTCCAGCAACGc                                <  2:848013/149‑1 (MQ=255)                                                                                                                tGCCCGTATTTACCATTAATTCGTCCAGCTGCATTTTCGAGCTGATGACGAATATCCTGATAGGCTTGCACATCACCACGCGACGTTGGTGCAATCTGGGTATAACGAATTTTACCATGATGCTGCGGATATTTTTCCAGCAACGCTTCa                            <  2:792745/150‑1 (MQ=255)                                                                                                                   ccGTATTTACCATTAATTCGTCCAGCTGCATTTTCGAGCTGATGACGAATATCCTGATAGGCTTGCACATCACCACGCGACGTTGGTGCAATCTGGGTATAACGAATTTTACCATGATGCTGCGGATATTTTTCCAGCAACGCTTCATAgg                        >  2:582298/1‑151 (MQ=255)                                                                                                                      tATTTACCATTAATTCGTCCAGCTGCATTTTCGAGCTGATGACGAATATCCTGATAGGCTTGCACATCACCACGCGACGTTGGTGCAATCTGGGTATAACGAATTTTACCATGATGCTGCGGATATTTTTCCAGCAACGCTTCATAGGCg                      >  2:492651/1‑150 (MQ=255)                                                                                                                          tACCATTAATTCGTCCAGCTGCATTTTCGAGCTGATGACGAATATCCTGATAGGCTTGCACATCACCACGCGACGTTGGTGCAATCTGGGTATAACGAATTTTACCATGATGCTGCGGATATTTTTCCAGCAACGCTTCATAGGCGAGaa                  >  1:72199/1‑150 (MQ=255)                                                                                                                           aCCATTAATTCGTCCAGCTGCATTTTCGAGCTGATGACGAATATCCTGATAGGCTTGCACATCACCACGCGACGTTGGTGCAATCTGGGTATAACGAATTTTACCATGATGCTGCGGATATTTTTCCAGCAACGCTTCATAGGCGAGaaa                 <  1:564233/150‑1 (MQ=255)                                                                                                                             cATTAATTCGTCCAGCTGCATTTTCGAGCTGATGACGAATATCCTGATAGGCTTGCACATCACCACGCGACGTTGGTGCAATCTGGGTATAACGAATTTTACCATGATGCTGCGGATATTTTTCCAGCAACGCTTCATAGGCGAGAAAACg              <  1:472410/151‑1 (MQ=255)                                                                                                                              aTTAATTCGTCCAGCTGCATTTTCGAGCTGATGACGAATATCCTGATAGGCTTGCACATCACCACGCGACGTTGGTGCAATCTGGGTATAACGAATTTTACCATGATGCTGCGGATATTTTTCCAGCAACGCTTCATAGGCGAGAAAACGc             <  1:882073/151‑1 (MQ=255)                                                                                                                              aTTAATTCGACCAGCTGCATTTTCGAGCTGATGACGAATATCCTGATAGGCTTGCACATCACCACGCGACGTTGGTGCAATCTGGGTATAACGAATTTTACCATGATGCTGCGGATATTTTTCCAGCAACGCTTCATAGGCGAGAAAAc               >  1:697281/1‑149 (MQ=255)                                                                                                                                  aTTCGTCCAGCTGCATTTTCGAGCTGATGACGAATATCCTGATAGGCTTGCACATCACCACGCGACGTTGGTGCAATCTGGGTATAACGAATTTTACCATGATGCTGCGGATATTTTTCCAGCAACGCTTCATAGGCGAGAAAACGCTCTg         <  1:829849/151‑1 (MQ=255)                                                                                                                                   ttCGTCCAGCTGCATTTTCGAGCTGATGACGAATATCCTGATAGGCTTGCACATCACCACGCGACGTTGGTGCAATCTGGGTATAACGAATTTTACCATGATGCTGCGGATATTTTTCCAGCAACGCTTCATAGGCGAGAAAACGCTCTgg        >  2:925418/1‑151 (MQ=255)                                                                                                                                     cGTCCAGCTGCATTTTCGAGCTGATGACGAATATCCTGATAGGCTTGCACATCACCACGCGACGTTGGTGCAATCTGGGTATAACGAATTTTACCATGATGCTGCGGATATTTTTCCAGCAACGCTTCATAGGCGAGAAAACGCTCTGGc       >  2:466855/1‑150 (MQ=255)                                                                                                                                      gTCCAGCTGCATTTTCGAGCTGATGACGAATATCCTGATAGGCTTGCACATCACCACGCGACGTTGGTGCAATCTGGGTATAACGAATTTTACCATGATGCTGCGGATATTTTTCCAGCAACGCTTCATAGGCGAGAAAACGCTCTGGCaa     <  2:45915/151‑1 (MQ=255)                                                                                                                                      gTCCAGCTGCATTTTCGAGCTGATGACGAATATCCTGATAGGCTTGCACATCACCACGCGACGTTGGTGCAATCTGGGTATAACGAATTTTACCATGATGCTGCGGATATTTTTCCAGCAACGCTTCATAGGCGAGAAAACGCTCTGGCa      >  1:985/1‑150 (MQ=255)                                                                                                                                        ccAGCTGCATTTTCGAGCTGATGACGAATATCCTGATAGGCTTGCACATCACCACGCGACGTTGGTGCAATCTGGGTATAACGAATTTTACCATGATGCTGCGGATATTTTTCCAGCAACGCTTCATAGGCGAGAAAACGCTCTGGCAAAc   <  2:493928/151‑1 (MQ=255)                                                                                                                                          aGCTGCATTTTCGAGCTGATGACGAATATCCTGATAGGCTTGCACATCACCACGCGACGTTGGTGCAATCTGGGTATAACGAATTTTACCATGATGCTGCGGATATTTTTCCAGCAACGCTTCATAGGCGAGAAAACGCTCTGGCAAAcc  <  1:188007/150‑1 (MQ=255)                                                                                                                                              |                                                                                                                                                  ATCCCGTCACGCAGTGGCGTCACTAAGCCCACGTCAGAGTAGCGGAATATTTTCATCAGTAATTTACGGTCAAAATGCTGATTCAAATAATAAAGCGGCGTCCAGCCTAATTGCCCGTATTTACCATTAATTCGTCCAGCTTCATTTTCGAGCTGATGACGAATATCCTGATAGGCTTGCACATCACCACGCGACGTTGGTGCAATCTGGGTATAACGAATTTTACCATGATGCTGCGGATATTTTTCCAGCAACGCTTCATAGGCGAGAAAACGCTCTGGCAAACC  >  NC_000913/1980522‑1980808

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑