Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,317,2490AC50.0% 3.6 / 67.8 44D389E (GAT→GAG) trpBtryptophan synthase, beta subunit
Reads supporting (aligned to +/- strand):  ref base A (13/9);  new base C (8/14);  total (21/23)
Fisher's exact test for biased strand distribution p-value = 2.27e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCGTATCGATAATTTTCAATGACTGCTCAATGCCCGGATCACCGAGCGTGACGAAAGGAACGAATGCGCCTTCTTTGCGCTCCTTCAACTGGGCAAACAGAGATTCGTAGCGTTCCATCAGATTTCCCCTCGTGCTTTCAAAATATCGTGAACGGTGAAGATGTCTTTATCGCCGCGACCGGAAAGGTTAACCACCAGTAGCTGCTCTTTATCCGGGTTTTCGCGCATCATTTTCAACGCATGGGCCAGGGCGTGGGAGGATTCCAGCGCCGGGATGATCCCTTCGT  >  NC_000913/1317105‑1317391
                                                                                                                                                |                                                                                                                                              
gCGTATCGATAATTTTCAATGACTGCTCAATGCCCGGATCACCGAGCGTGACGAAAGGAACGAATGCGCCTTCTTTGCGCTCCTTCAACTGGGCAAACAGAGATTCGTAGCGTTCCATCAGATTTCCCCTCGTGCTTTCAAAATCTCGTGa                                                                                                                                          <  1:25827/151‑1 (MQ=255)
    aTCGATAATTTTCAATGACTGCTCAATGCCCGGATCACCGAGCGTGACGAAAGGAACGAATGCGCCTTCTTTGCGCTCCTTCAACTGGGCAAACAGAGATTCGTAGCGTTCCATCAGATTTCCCCTCGTGCTTTCAAAATCTCGTGAACg                                                                                                                                       <  1:97496/150‑1 (MQ=255)
      cGATAATTTTCAATGACTGCTCAATGCCCGGATCACCGAGCGTGACGAAAGGAACGAATGCGCCTTCTTTGCGCTCCTTCAACTGGGCAAACAGAGATTCGTAGCGTTCCATCAGATTTCCCCTCGTGCTTTCAAAATATCGTGAACGGt                                                                                                                                     <  1:75337/150‑1 (MQ=255)
        aTAATTTTCAATGACTGCTCAATGCCCGGATCACCGAGCGTGACGAAAGGAACGAATGCGCCTTCTTTGCGCTCCTTCAACTGGGCAAACAGAGATTCGTAGCGTTCCATCAGATTTCCCCTCGTGCTTTCAAAATCTCGTGAACGGTGa                                                                                                                                   >  1:451454/1‑150 (MQ=255)
         tAATTTTCAATGACTGCTCAATGCCCGGATCACCGAGCGTGACGAAAGGAACGAATGCGCCTTCTTTGCGCTCCTTCAACTGGGCAAACAGAGATTCGTAGCGTTCCATCAGATTTCCCCTCGTGCTTTCAAAATATCGTGAACGGTGa                                                                                                                                   >  2:921624/1‑149 (MQ=255)
          aaTTTTCAATGACTGCTCAATGCCCGGATCACCGAGCGTGACGAAAGGAACGAATGCGCCTTCTTTGCGCTCCTTCAACTGGGCAAACAGAGATTCGTAGCGTTCCATCAGATTTCCCCTCGTGCTTTCAAAATCTCGTGAACGGTGAAg                                                                                                                                 <  2:775003/150‑1 (MQ=255)
          aaTTTTCAATGACTGCTCAATGCCCGGATCACCGAGCGTGACGAAAGGAACGAATGCGCCTTCTTTGCGCTCCTTCAACTGGGCAAACAGAGATTCGTAGCGTTCCATCAGATTTCCCCTCGTGCTTTCAAAATATCGTGAACGGTGa                                                                                                                                   >  1:574384/1‑148 (MQ=255)
               tCAATGACTGCTCAATGCCCGGATCACCGAGCGTGACGAAAGGAACGAATGCGCCTTCTTTGCGCTCCTTCAACTGGGCAAACAGAGATTCGTAGCGTTCCATCAGATTTCCCCTCGTGCTTTCAAAATATCGTGAACGGTGAAGATGTc                                                                                                                            <  1:282324/150‑1 (MQ=255)
                  aTGACTGCTCAATGCCCGGATCACCGAGCGTGACGAAAGGAACGAATGCGCCTTCTTTGCGCTCCTTCAACTGGGCAAACAGAGATTCGTAGCGTTCCATCAGATTTCCCCTCGTGCTTTCAAAATATCGTGAACGGTGAAGATGTCttt                                                                                                                         >  2:772870/1‑150 (MQ=255)
                        gCTCAATGCCCGGATCACCGAGCGTGACGAAAGGAACGAATGCGCCTTCTTTGCGCTCCTTCAACTGGGCAAACAGAGATTCGTAGCGTTCCATCAGATTTCCCCTCGTGCTTTCAAAATATCGTGAACGGTGAAGATGTCTTTATcgc                                                                                                                    <  1:111060/149‑1 (MQ=255)
                          tCAATGCCCGGATCACCGAGCGTGACGAAAGGAACGAATGCGCCTTCTTTGCGCTCCTTCAACTGGGCAAACAGAGATTCGTAGCGTTCCATCAGATTTCCCCTCGTGCTTTCAAAATATCGTGAACGGTGAAGATGTCTTTATCGCcgcg                                                                                                                >  1:498436/1‑151 (MQ=255)
                                       cACCGAGCGTGACGAAAGGAACGAATGCGCCTTCTTTGCGCTCCTTCAACTGGGCAAACAGAGATTCGTAGCGTTCCATCAGATTTCCCCTCGTGCTTTCAAAATCTCGTGAACGGTGAAGATGTCTTTATCGCCGCGACCGGAAAGGtt                                                                                                    >  1:180226/1‑150 (MQ=255)
                                       cACCGAGCGTGACGAAAGGAACGAATGCGCCTTCTTTGCGCTCCTTCAACTGGGCAAACAGAGATTCGTAGCGTTCCATCAGATTTCCCCTCGTGCTTTCAAAATCTCGTGAACGGTGAAGATGTCTTTATCGCCGCGACCGGAAAGGTTa                                                                                                   <  1:152694/151‑1 (MQ=255)
                                         ccGAGCGTGACGAAAGGAACGAATGCGCCTTCTTTGCGCTCCTTCAACTGGGCAAACAGAGATTCGTAGCGTTCCATCAGATTTCCCCTCGTGCTTTCAAAATCTCGTGAACGGTGAAGATGTCTTTATCGCCGCGACCGGAAAGGTTAac                                                                                                 >  1:321681/1‑151 (MQ=255)
                                           gAGCGTGACGAAAGGAACGAATGCGCCTTCTTTGCGCTCCTTCAACTGGGCAAACAGAGATTCGTAGCGTTCCATCAGATTTCCCCTCGTGCTTTCAAAATATCGTGAACGGTGAAGATGTCTTTATCGCCGCGACCGGAAAGGTTAacc                                                                                                >  1:638766/1‑150 (MQ=255)
                                                 gACGAAAGGAACGAATGCGCCTTCTTTGCGCTCCTTCAACTGGGCAAACAGAGATTCGTAGCGTTCCATCAGATTTCCCCTCGTGCTTTCAAAATCTCGTGAACGGTGAAGATGTCTTTATCGCCGCGACCGGAAAGGTTAACCACCAGTa                                                                                         <  2:529441/151‑1 (MQ=255)
                                                 gACGAAAGGAACGAATGCGCCTTCTTTGCGCTCCTTCAACTGGGCAAACAGAGATTCGTAGCGTTCCATCAGATTTCCCCTCGTGCTTTCAAAATATCGTGAACGCTGAAGATGTCTTTATCGCCGCGACCGAAAAGGTTAACCACCAg                                                                                           >  2:406067/1‑149 (MQ=255)
                                                  aCGAAAGGAACGAATGCGCCTTCTTTGCGCTCCTTCAACTGGGCAAACAGAGATTCGTAGCGTTCCATCAGATTTCCCCTCGTGCTTTCAAAATATCGTGAACGGTGAAGATGTCTTTATCGCCGCGACCGGAAAGGTTAACCACCAGt                                                                                          >  1:231036/1‑149 (MQ=255)
                                                    gAAAGGAACGAATGCGCCTTCTTTGCGCTCCTTCAACTGGGCAAACAGAGATTCGTAGCGTTCCATCAGATTTCCCCTCGTGCTTTCAAAATCTCGTGAACGGTGAAGATGTCTTTATCGCCGCGACCGGAAAGGTTAACCACCAGTAgct                                                                                      <  2:842999/151‑1 (MQ=255)
                                                          aaCGAATGCGCCTTCTTTGCGCTCCTTCAACTGGGCAAACAGAGATTCGTAGCGTTCCATCAGATTTCCCCTCGTGCTTTCAAAATCTCGTGAACGGTGAAGATGTCTTTATCGCCGCGACCGGAAAGGTTAACCACCAGTAGCTGCTCtt                                                                                <  2:252368/151‑1 (MQ=255)
                                                              aaTGCGCCTTCTTTGCGCTCCTTCAACTGGGCAAACAGAGATTCGTAGCGTTCCATCAGATTTCCCCTCGTGCTTTCAAAATATCGTGAACGGTGAAGATGTCTTTATCGCCGCGACCGGAAAGGTTAACCACCAGTAGCTGCTCTTtctc                                                                            >  2:34035/1‑151 (MQ=255)
                                                                  cgcCTTCTTTGCGCTCCTTCAACTGGGCAAACAGAGATTCGTAGCGTTCCATCAGATTTCCCCTCGTGCTTTCAAAATCTCGTGAACGGTGAAGATGTCTTTATCGCCGCGACCGGAAAGGTTAACCACCAGTAGCTGCTCTTTCTCCggg                                                                        <  1:338977/151‑1 (MQ=255)
                                                                     cttcttTGCGCTCCTTCAACTGGGCAAACAGAGATTCGTAGCGTTCCATCAGATTTCCCCTCGTGCTTTCAAAATCTCGTGAACGGTGAAGATGTCTTTATCGCCGCGACCGGAAAGGTTAACCACCAGTAGCTGCTCTTTCTCCGGGttt                                                                     >  1:290050/1‑151 (MQ=255)
                                                                        cttTGCGCTCCTTCAACTGGGCAAACAGAGATTCGTAGCGTTCCATCAGATTTCCCCTCGTGCTTTCAAAATCTCGTGAACGGTGAAGATGTCTTTATCGCCGCGACCGGAAAGGTTAACCACCAGTAGCTGCTCTTTCTCCGGGTTTTcg                                                                  >  1:723360/1‑151 (MQ=255)
                                                                            gcgcTCCTTCAACTGGGCAAACAGAGATTCGTAGCGTTCCATCAGATTTCCCCTCGTGCTTTCAAAATATCGTGAACGGTGAAGATGTCTTTATCGCCGCGACCGGAAAGGTTAACCACCAGTAGCTGCTCTTTCTCCGGGTTTTCgcgc                                                               <  1:29408/150‑1 (MQ=255)
                                                                                tCCTTCAACTGGGCAAACAGAGATTCGTAGCGTTCCATCAGATTTCCCCTCGTGCTTTCAAAATATCGTGAACGGTGAAGATGTCTTTATCGCCGCGACCGGAAAGGTTAACCACCAGTAGCTGCTCTTTCTCCGGGTTTTCGCGcatcat                                                          >  2:203664/1‑151 (MQ=255)
                                                                                  cTTCAACTGGGCAAACAGAGATTCGTAGCGTTCCATCAGATTTCCCCTCGTGCTTTCAAAATCTCGTGAACGGTGAAGATGTCTTTATCGCCGCGACCGGAAAGGTTAACCACCAGTAGCTGCTCTTTCTCCGGGTTTTCGCGCATCAttt                                                        >  1:387576/1‑151 (MQ=255)
                                                                                     cAACTGGGCAAACAGAGATTCGTAGCGTTCCATCAGATTTCCCCTCGTGCTTTCAAAATATCGTGAACGGTGAAGATGTCTTTATCGCCGCGACCGGAAAGGTTAACCACCAGTAGCTGCTCTTTCTCCGGGTTTTCGCGCATCATTTTc                                                      <  2:160361/150‑1 (MQ=255)
                                                                                     cAACTGGGCAAACAGAGATTCGTAGCGTTCCATCAGATTTCCCCTCGTGCTTTCAAAATATCGTGAACGGTGAAGATGTCTTTATCGCCGCGACCGGAAAGGTTAACCACCAGTAGCTGCTCTTTCTCCGGGTTTTCGCGCATCATTTTCa                                                     <  2:900962/151‑1 (MQ=255)
                                                                                       aCTGGGCAAACAGAGATTCGTAGCGTTCCATCAGATTTCCCCTCGTGCTTTCAAAATATCGTGAACGGTGAAGATGTCTTTATCGCCGCGACCGGAAAGGTTAACCACCAGTAGCTGCTCTTTCTCCGGGTTTTCGCGCATCATTTTCAAc                                                   >  1:781901/1‑151 (MQ=255)
                                                                                         tGGGCAAACAGAGATTCGTAGCGTTCCATCAGATTTCCCCTCGTGCTTTCAAAATCTCGTGAACGGTGAAGATGTCTTTATCGCCGCGACCGGAAAGGTTAACCACCAGTAGCTGCTCTTTCTCCGGGTTTTCGCGCATCATTTTCAACGc                                                 >  2:281603/1‑151 (MQ=255)
                                                                                          gggCAAACAGAGATTCGTAGCGTTCCATCAGATTTCCCCTCGTGCTTTCAAAATCTCGTGAACGGTGAAGATGTCTTTATCGCCGCGACCGGAAAGGTTAACCACCAGTAGCTGCTCTTTCTCCGGGTTTTCGCGCATCATTTTCAACg                                                  <  1:592550/149‑1 (MQ=255)
                                                                                              aaaCAGAGATTCGTAGCGTTCCATCAGATTTCCCCTCGTGCTTTCAAAATCTCGTGAACGGTGAAGATGTCTTTATCGCCGCGACCGGAAAGGTTAACCACCAGTAGCTGCTCTTTCTCCGGGTTTTCGCGCATCATTTTCAACGCATgg                                             >  1:135455/1‑150 (MQ=255)
                                                                                                 cAGAGATTCGTAGCGTTCCATCAGATTTCCCCTCGTGCTTTCAAAATCTCGTGAACGGTGAAGATGTCTTTATCGCCGCGACCGGAAAGGTTAACCACCAGTAGCTGCTCTTTCTCCGGGTTTTCGCGCATCATTTTCAACGCATGGGCCa                                         <  1:912545/151‑1 (MQ=255)
                                                                                                 cAGAGATTCGTAGCGTTCCATCAGATTTCCCCTCGTGCTTTCAAAATATCGTGAACGGTGAAGATGTCTTTATCGCCGCGACCGGAAAGGTTAACCACCAGTAGCTGCTCTTTCTCCGGGTTTTCGCGCATCATTTTCAACGCATGGGCCa                                         <  1:547658/151‑1 (MQ=255)
                                                                                                   gagaTTCGTAGCGTTCCATCAGATTTCCCCTCGTGCTTTCAAAATCTCGTGAACGGTGAAGATGTCTTTATCGCCGCGACCGGAAAGGTTAACCACCAGTAGCTGCTCTTTCTCCGGGTTTTCGCGCATCATTTTCAACGCATGGGCCAgg                                       <  2:69028/151‑1 (MQ=255)
                                                                                                     gaTTCGTAGCGTTCCATCAGATTTCCCCTCGTGCTTTCAAAATCTCGTGAACGGTGAAGATGTCTTTATCGCCGCGACCGGAAAGGTTAACCACCAGTAGCTGCTCTTTCTCCGGGTTTTCGCGCATCATTTTCAACGCATGGGCCAGGGc                                     <  2:431033/151‑1 (MQ=255)
                                                                                                          gTAGCGTTCCATCAGATTTCCCCTCGTGCTTTCAAAATATCGTGAACGGTGAAGATGTCTTTATCGCCGCGACCGGAAAGGTTAACCACCAGTAGCTGCTCTTTCTCCGGGTTTTCGCGCATCATTTTCAACGCATGGGCCAGGGCGTggg                                <  1:518363/151‑1 (MQ=255)
                                                                                                             gCGTTCCATCAGATTTCCCCTCGTGCTTTCAAAATATCGTGAACGGTGAAGATGTCTTTATCGCCGCGACCGGAAAGGTTAACCACCAGTAGCTGCTCTTTCTCCGGGTTTTCGCGCATCATTTTCAACGCATGGGCCAGGGCGTGggagg                             >  1:382868/1‑151 (MQ=255)
                                                                                                               gTTCCATCAGATTTCCCCTCGTGCTTTCAAAATATCGTGAACGGTGAAGATGTCTTTATCGCCGCGACCGGAAAGGTTAACCACCAGTAGCTGCTCTTTCTCCGGGTTTTCGCGCATCATTTTCAACGCATGGGCCAGGGCGTGGGAGGAt                           <  1:899241/151‑1 (MQ=255)
                                                                                                                       aGATTTCCCCTCGTGCTTTCAAAATCTCGTGAACGGTGAAGATGTCTTTATCGCCGCGACCGGAAAGGTTAACCACCAGTAGCTGCTCTTTCTCCGGGTTTTCGCGCATCATTTTCAACGCATGGGCCAGGGCGTGGGAGGATTCCAgcgc                   <  2:174192/151‑1 (MQ=255)
                                                                                                                       aGATTTCCCCTCGTGCTTTCAAAATCTCGTGAACGGTGAAGATGTCTTTATCGCCGCGACCGGAAAGGTTAACCACCAGTAGCTGCTCTTTCTCCGGGTTTTCGCGCATCATTTTCAACGCATGGGCCAGGGCGTGGGAGGATTCCAgcgc                   <  2:899006/151‑1 (MQ=255)
                                                                                                                          tttCCCCTCGTGCTTTCAAAATATCGTGAACGGTGAAGATGTCTTTATCGCCGCGACCGGAAAGGTTAACCACCAGTAGCTGCTCTTTCTCCGGGTTTTCGCGCATCATTTTCAACGCATGGGCCAGGGCGTGGGAGGATTCCAGCGCCgg                >  2:474882/1‑151 (MQ=255)
                                                                                                                                        ttCAAAATATCGTGAACGGTGAAGATGTCTTTATCGCCGCGACCGGAAAGGTTAACCACCAGTAGCTGCTCTTTCTCCGGGTTTTCGCGCATCATTTTCAACGCATGGGCCAGGGCGTGGGAGGATTCCAGCGCCGGGATGATCCCTTCGt  >  1:676184/1‑151 (MQ=255)
                                                                                                                                                |                                                                                                                                              
GCGTATCGATAATTTTCAATGACTGCTCAATGCCCGGATCACCGAGCGTGACGAAAGGAACGAATGCGCCTTCTTTGCGCTCCTTCAACTGGGCAAACAGAGATTCGTAGCGTTCCATCAGATTTCCCCTCGTGCTTTCAAAATATCGTGAACGGTGAAGATGTCTTTATCGCCGCGACCGGAAAGGTTAACCACCAGTAGCTGCTCTTTATCCGGGTTTTCGCGCATCATTTTCAACGCATGGGCCAGGGCGTGGGAGGATTCCAGCGCCGGGATGATCCCTTCGT  >  NC_000913/1317105‑1317391

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑