Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 3,947,831 | 0 | T | G | 45.5% | 83.1 / 15.1 | 57 | N46T (AAC→ACC) | hdfR | flhDC operon transcriptional repressor |
Reads supporting (aligned to +/- strand): ref base T (5/25); new base G (25/0); total (32/25) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 8.87e-11 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
CACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGCGGGTGAAAAGGTTCACACCCAGTTGATTTTCCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGGTCAGATAGAGCGATTCAGCCGCTCGACCAAAGTGACGCGTTCGGCTAACTTCCAGGAAAGTTTTTAACAATTCCGTATCCACAGTGCTCTCCGC > NC_000913/3947685‑3947979 | cACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGCGGGTGAAAAGGTTcac < 1:754004/151‑1 (MQ=255) gTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGCGGGTGAAAAGGTTCACACCCAGTTGATTTTcc < 1:315617/151‑1 (MQ=255) tATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGCGGGGGAAAAGGGTCACACCCCGTTGAttttt > 2:664679/1‑147 (MQ=255) tATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGCGGGGGAAAAAGGTTACACCCAGTTGAtttt > 2:707501/1‑147 (MQ=255) gTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGCGGGTGAAAAGGTTCACACCCAGTTGATTTTCCAGTTg < 1:314101/151‑1 (MQ=255) ggTATGCGCCGCCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGCGGGTGAAAAGGTTCACACCCAGTTGATTTTCCAGTTGTCTGAtt < 1:449288/151‑1 (MQ=255) aTGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGCGGGTGAAAAGGATCCCACCCCGTTGATTTTTCAGTTGTCTg > 1:100230/1‑145 (MQ=255) gcgcCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGCGGGTGAAAAGGTTCACACCCAGTTGATTTTCCAGTTGTCTGATTCGaaa < 1:435785/151‑1 (MQ=255) gcgcCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGCGGGTGAAAAGGTTCACACCCAGTTGATTTTCCAGTTGTCTGATTCGaaa < 2:701070/151‑1 (MQ=255) gcgcCACCTCCTTACGGGCGGCCTGCCACGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTTCTTCTGTCGCGGGTGAAAAGGTTCACACCCAGTTGATTTTCCAGTTGTCTGATTCGaa < 2:755628/150‑1 (MQ=255) cgcCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGCGGGGGAAAAAGGTTACACCCCGTTGATTTTTCAGTTGTTTTATTTGaaa > 2:602383/1‑150 (MQ=255) cctcctTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGCGGGGGAAAAGGGTCACACCCCGTTGATTTTTCCGTTGTCTGATTTGAAAACTCAc > 1:489198/1‑151 (MQ=255) tcctTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGCGGGGGTAAAAGGTCACAACCCGTTGATTTTTCCGTTGTTTGATTTTAAAACTCAc > 1:6023/1‑149 (MQ=255) gggCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGCGGGTGAAAAAGGTTACACCCCGGTGATTTTTCTGTTGTTTGGTTTGAAAAGCCACTGCTGACTg > 2:491105/1‑151 (MQ=255) ggcggcCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGCGGGCGAAAAGGTGCACAACCAGGTGGTTTTTCAGTTGTCTGGTTCGAAAGCGCACTGCCGGCTgg > 1:334589/1‑151 (MQ=255) gcggcCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGCGGGTGAAAAGGTTCACACCCAGTTGATTTTCCAGTTGTCTGATTCGAAAGCTCACTGCTGACTggg < 2:417913/151‑1 (MQ=255) ggcCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGCGGGTGAAAAAGGTTACACCCCGTTGATTTTTCCGTTTTCTGATTCTAAAACTCCCTGCTGGCTGGGt > 2:445793/1‑150 (MQ=255) ccAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGCGGGGGGAAAAGGTCACACCCCAGTGATTTTTCAGTTGTCTGGTTTGAAAAGTCACTGCTGGCTGGGTGAg > 2:320749/1‑147 (MQ=255) cTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGCGGGGGAAAAGGGTCACACCCCGTTGATTTTTCCGTTGTCTGATTTGAAAACTCACTGCTGACTGGGTCAGATAAAGCGGtt > 1:467611/1‑151 (MQ=255) cGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGCGGGTGAAAAAGGTTACACCCCGTTGATTTTTCCCTTGTCTGATTTGAAAGCTCACTGCTGACTGGGTCAGATAGAGCGATTCAGcc > 1:496720/1‑148 (MQ=255) gTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGCGGGTGAAAAAGGTTACACCCAGGTGATTTTTCAGTTTTTTGATTTGAAAGCCCACTGCTGACCGGGGCAGATAGAGGGATTTAGcccccc > 1:904654/1‑147 (MQ=255) cTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGCGGGGGAAAAGGGTCACACCCCGGTGTTTTTTCAGCTGTCTGATTCGAAAACTCACTGCTGGCTGGGGCAGATAGAGCGATTTAGCCGCCCGAc > 1:713657/1‑150 (MQ=255) tGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGCGGGGGGAAAGGTTCCCACCCCGTTGATTTTTTCGTTGTCTTATTCGTAAACTCACTGCTGACCGGGTCAGATAGAGGGGTTTAGGCGCTccgcc > 2:337288/1‑146 (MQ=255) cATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGCGGGGGAAAAGGGTCACACCCAGTTGATTTTTCCGTTGTCTGATTCGAAAACTCACTGCTGACTGGGGCAGATAGAGGGATTCAGCCGCCCGACCaa > 1:238725/1‑150 (MQ=255) aaGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGCGGGGGAAAAAGGTCACACCCCGTTGGTTTTTCAGTTGTTTTGTTTGAAAACTCACTGCTGACTGGGGCAGATAAAGGGATTTAGCCCCTCCACCcaa > 2:474640/1‑148 (MQ=255) ggCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGCGGGGTGAAAGGGTCACACCCAGTTGATTTTTCCGTTTTCTGTTTATAAAACTCACTGCTGACTGGGTCATGAAGAGCGATTTAGCCGCACGACCCAAATg > 1:543308/1‑149 (MQ=255) gCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGCGGGGGGAAAAGATCCCCCCCAGTTGTTTTTTCCGTTTTCTGATTCCAAAACCCACAGCTGCCTGGGTCGGAGAGCACGATTCAGCCCCTCGaacca > 1:251266/1‑140 (MQ=255) agtTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGCGGGTGAAAAGGTTCACACCCAGTTGATTTTCCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGGTCAGATAGAGCGATTCAGCCGCTCGACCAAAGTGACGCGTTc < 2:176431/151‑1 (MQ=255) gtTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGCGGGTGAAAAGGTTCACACCCAGTTGATTTTCCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGGTCAGATAGAGCGATTCAGCCGCTCGACCAAAGTGACGCGTTCg < 1:212730/151‑1 (MQ=255) tttCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGCGGGTGAAAAGGTTCACACCCAGTTGATTTTCCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGGTCAGATAGAGCGATTCAGCCGCTCGACCAAAGTGACGCGTTCGGc < 1:467881/150‑1 (MQ=255) ttCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGCGGGTGAAAAGGTTCACACCCAGTTGATTTTCCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGGTCAGATAGAGCGATTCAGCCGCTCGACCAAAGTGACGCGTTCGGCt < 2:671421/150‑1 (MQ=255) ttCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGCGGGTGAAAAGGTTCACACCCAGTTGATTTTCCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGGTCAGATAGAGCGATTCAGCCGCTCGACCAAAGTGACGCGTTCGGCt < 2:861152/150‑1 (MQ=255) ttCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGCGGGTGAAAAGGGTTACACCCCGTTTATTTTTCCGTTGTCTGATTTGAAAGCTCACTGCTGGCTGGGGCAGATAGAGCGATTCAGCCGCTCCACCCAAAAGAcgcggt > 1:482696/1‑143 (MQ=255) aCCGGCAGCGGTTAAACGGATATTGTTTCTGTGGCGGGTGAAAAGGGTCACACCCAGGTGATTTTTCAGTTGTCTGGTTCGAAAACTCACTGGTGACTGGGTCAGATAGAGCGATTCAGCCGCTCGCCCAAAAAGAAGCGGTCGGCCaa > 2:47390/1‑149 (MQ=255) aCCGGCAGCGGTTAAACGGATATTGTTTCTGTGGCGGGTGAAAAAGGTCACACCCAGTTGATTTTTCAGTTGTCTGATTCGAAAACTCACTGCTGACTGGGGCAGATAGAGCGATTCAGCCGCCCGACCAAAGTGACGCGTTCGGCTAAc > 1:669320/1‑150 (MQ=255) aCCGGCAGCGGTTAAACGGATATTGTTTCTGTGGCGGGGGAAAAAGGTCACACCCCGTTGATTTTTCCGTTGTTTGGTTTGAAAACTCCCTGCTGGCTGGGGGAGATAGAGCGGTTTAGCCGCCCGGCCCAAGTGACCCGGTCGGGTaa > 2:156740/1‑149 (MQ=255) ggTTAAACGGATATTGTTTCTGTGGCGGGGGAAAAAGGTCACACCCAGTTGATTTTCCAGTTGTCTGATTCGAAAACTCACTGCTGACTGGGGCAGATAGAGCGATTCAGCCGCCCGACCAAAGTGACGCGTTCGGCTAACTTCCAGGaa > 1:108578/1‑150 (MQ=255) gTTAAACGGATATTGTTTCTGTGGCGGGTGAAAAGGGTCACACCCAGTTGATTTTTCCGTTGTCTGATTCGAAAGCTCACTGCTGACTGGGGCAGATAGAGCGATTCAGCCGCTCGACCAAAGTGACGCGGTTGGCTAACTTCCAGGaa > 1:820841/1‑149 (MQ=255) ttAAACGGATATTGTTTCTGTGGCGGGGGAAAAAGGTCCCACCCCGGTGATTTTTTACTTGTTTGGTTTGAAAAATCACTGCTGAATGGGGCAGATAGAGGGGTTTAGCCGCTCGACCAAAAAGACGGGTTCGGCTAACTTCCAGGaa > 2:66988/1‑148 (MQ=255) aaaCGGATATTGTTTCTGTGGCGGGTGAAAAGGTTCACACCCAGTTGATTTTCCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGGTCAGATAGAGCGATTCAGCCGCTCGACCAAAGTGACGCGTTCGGCTAACTTCCAGGAAAGttt < 1:841299/151‑1 (MQ=255) aaaCGGATATTGTTTCTGTGGCGGGTGAAAAGGTTCACACCCAGTTGATTTTCCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGGTCAGATAGAGCGATTCAGCCGCTCGACCAAAGTGACGCGTTCGGCTAACTTCCAGGAAAGtt < 1:469502/150‑1 (MQ=255) aCGGATATTGTTTCTGTGGCGGGTGAAAAGGTTCACACCCAGTTGATTTTCCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGGTCAGATAGAGCGATTCAGCCGCTCGACCAAAGTGACGCGTTCGGCTAACTTCCAGGAAAGttttt < 2:866492/151‑1 (MQ=255) aCGGATATTGTTTCTGTGGCGGGGGAAAAAGGTCCCACCCAGTTGATTTTTCAGTTTTTTGGTTTGAAAACTCACTGCTGACTGGGGCAGATAGAGGGATTTAGCCGCCCGACCAAAGTGACGCGGTTGGGTAACTTCCAGGGAAGatttt > 2:326320/1‑151 (MQ=255) cGGATATTGTTTCTGTGGCGGGTGAAAAGGTTCACACCCAGTTGATTTTCCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGGTCAGATAGAGCGATTCAGCCGCTCGACCAAAGTGACGCGTTCGGCTAACTTCCAGGAAAGTTTTTa < 1:493303/151‑1 (MQ=255) cGGATATTGTTTCTGTGGCGGGTGAAAAAGGTTACACCCAGGTGATTTTTCAGTTGTCTGGTTTGGAAAATCACTGCTGGCTGGGGCAGATAGAGCGATTTAGCCGCCCGACCAAAAGGAAGCGGTCGGCTTACTTCCAGGAAAGgtttt > 2:458300/1‑150 (MQ=255) atTGTTTCTGTGGCGGGTGAAAAGGTTCACACCCAGTTGATTTTCCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGGTCAGATAGAGCGATTCAGCCGCTCGACCAAAGTGACGCGTTCGGCTAACTTCCAGGAAAGTTTTTAACaa < 1:352031/150‑1 (MQ=255) tGTTTCTGTGGCGGGTGAAAAGGTTCACACCCAGTTGATTTTCCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGGTCAGATAGAGCGATTCAGCCGCTCGACCAAAGTGACGCGTTCGGCTAACTTCCAGGAAAGTTTTTAACAAtt < 2:24096/150‑1 (MQ=255) ttCTGTGGCGGGTGAAAAGGTTCACACCCAGTTGATTTTCCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGGTCAGATAGAGCGATTCAGCCGCTCGACCAAAGTGACGCGTTCGGCTAACTTCCAGGAAAGTTTTTAACAATTCCg < 1:99821/150‑1 (MQ=255) tCTGTGGCGGGTGAAAAGGTTCACACCCAGTTGATTTTCCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGGTCAGATAGAGCGATTCAGCCGCTCGACCAAAGTGACGCGTTCGGCTAACTTCCAGGAAAGTTTTTAACAATTCCGt < 2:810913/150‑1 (MQ=255) gtgGCGGGTGAAAAGGTTCACACCCAGTTGATTTTTCAGTTGTCTGGTTCGCAAACTCACTGCCGACTGGGTCAGATAGAGCGATTTAGACGCTCGACCAAAGTGACGCGTTCGGCGAACATCCAGGAAAGTTTTTAAAAAtttc > 2:686650/1‑143 (MQ=255) gtgGCGGGTGAAAAGGTTCACACCCAGTTGATTTTCCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGGTCAGATAGAGCGATTCAGCCGCTCGACCAAAGTGACGCGTTCGGCTAACTTCCAGGAAAGTTTTTAACAATTCCGTATc < 2:221213/150‑1 (MQ=255) gtgGCGGGTGAAAAAGGTCACACCCAGGTGGTTTTTCCGTTGTTTGATTCGAAAACTCACTGCTGACTGGGTCAGATAGAGCGATTCAGCCGCTCGACCAAAAGGACGCGGTCGGCTAACTTCCAGGAAAGTTTTTAACAATTTCGTATcc > 2:567565/1‑151 (MQ=255) ggCGGGTGAAAAGGTTCACACCCAGTTGATTTTCCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGGTCAGATAGAGCGATTCAGCCGCTCGACCAAAGTGACGCGTTCGGCTAACTTCCAGGAAAGTTTTTAACAATTCCGTATCcac > 2:516730/1‑151 (MQ=255) gCGGGTGAAAAGGTTCACACCCAGTTGATTTTCCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGGTCAGATAGAGCGATTCAGCCGCTCGACCAAAGTGACGCGTTCGGCTAACTTCCAGGAAAGTTTTTAACAATTCCGTATCcaca < 2:632603/151‑1 (MQ=255) aaaGGTTCACACCCAGTTGATTTTCCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGGTCAGATAGAGCGATTCAGCCGCTCGACCAAAGTGACGCGTTCGGCTAACTTCCAGGAAAGTTTTTAACAATTCCGTATCCACAGTGCtctc < 1:241370/151‑1 (MQ=255) aGGTTCACACCCAGTTGATTTTCCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGGTCAGATAGAGCGATTCAGCCGCTCGACCAAAGTGACGCGTTCGGCTAACTTCCAGGAAAGTTTTTAACAATTCCGTATCCACAGTGCTCTcca < 1:248658/151‑2 (MQ=255) ggTTCACACCCAGTTGATTTTCCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGGTCAGATAGAGCGATTCAGCCGCTCGACCAAAGTGACGCGTTCGGCTAACTTCCAGGAAAGTTTTTAACAATTCCGTATCCACAGTGCTCTCCGc > 2:317372/1‑151 (MQ=255) | CACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTTAAACGGATATTGTTTCTGTGGCGGGTGAAAAGGTTCACACCCAGTTGATTTTCCAGTTGTCTGATTCGAAAGCTCACTGCTGACTGGGTCAGATAGAGCGATTCAGCCGCTCGACCAAAGTGACGCGTTCGGCTAACTTCCAGGAAAGTTTTTAACAATTCCGTATCCACAGTGCTCTCCGC > NC_000913/3947685‑3947979 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |